Composition for improving complications caused by female hormone drop and application

Abstract

The invention belongs to the technical field of medicine preparation, and particularly relates to a composition for improving complications caused by female hormone drop and application. According to the composition, the black rhizoma cimicifugae and the red clover are combined, so that the female in-vivo estrogen level can be effectively adjusted, and discomfort caused by hormone disorder is relieved; the prepared rehmannia root and the white paeony root are beneficial to moistening skin while improving qi and blood; peucedanum praeruptorum and platycodon grandiflorum are beneficial for improving the immunity of the body, calming the nerves and relieving anxiety. Therefore, the composition can be used for improving complications caused by female hormone drop.

Description

Technical Field

[0001] The present invention belongs to the technical field of drug preparation, and particularly relates to a composition and an application for improving complications caused by low female hormones. Background Art

[0002] Female hormones are secreted by the ovaries and are mainly responsible for female development and maintaining the health of the female reproductive system. However, due to different external factors and self-reasons, it may lead to disorders of female hormones secreted by the ovaries, resulting in a lack of hormones in women, and further may lead to a series of health problems and discomfort symptoms, seriously threatening the physical and mental health of patients.

[0003] In the prior art, sodium prasterone sulfate is mostly used to improve female hormones. For example, in Chinese Patent CN104983705 B and Chinese Patent CN 102429912 B, sodium prasterone sulfate is used as the active ingredient to improve female hormones. However, sodium prasterone sulfate is difficult to dissolve in water, so its application range is limited. Therefore, how to provide a drug with good effects and convenient use to improve female hormones will benefit thousands of women. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition and an application for improving complications caused by low female hormones, which can be used to improve complications caused by low female hormones and have the characteristics of convenient use and good effects.

[0005] The present invention provides a composition for improving complications caused by low female hormones, and the composition includes: black cohosh, white peony root, prepared rehmannia root, red clover, platycodon root and purple-flowered hogfennel root.

[0006] Preferably, by mass parts, the composition includes: 20 - 25 parts of black cohosh, 15 - 20 parts of white peony root, 10 - 15 parts of prepared rehmannia root, 8 - 10 parts of red clover, 8 - 10 parts of platycodon root and 7 - 8 parts of purple-flowered hogfennel root.

[0007] Preferably, by mass parts, the composition includes: 25 parts of black cohosh, 15 parts of white peony root, 10 parts of prepared rehmannia root, 8 parts of red clover, 8 parts of platycodon root and 7 parts of purple-flowered hogfennel root.

[0008] The present invention provides a preparation method of the composition according to the above technical solution, including the following steps:

[0009] Cimicifuga racemosa is mixed with ethanol having a volume concentration of 78%-85% and then subjected to first heating extraction to obtain a Cimicifuga racemosa extract; the Cimicifuga racemosa extract, Paeonia lactiflora Pall., Rehmannia glutinosa Libosch. and water are mixed and then subjected to steaming to obtain a first filtrate; the first filtrate is concentrated to obtain a first extract; Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC., Peucedanum praeruptorum Dunn and water are mixed and then successively subjected to second heating extraction and vacuum concentration to obtain a mixed extract; the mixed extract is dissolved with ethanol having a volume concentration of 90%-95%, the precipitate is removed and concentrated to obtain a second extract; the first extract and the second extract are mixed to obtain a composition for improving complications caused by low female hormones.

[0010] Preferably, when performing the first heating, the volume ratio of Cimicifuga racemosa to ethanol is 1:8-10;

[0011] The time for the first heating extraction is 2-3 h; the temperature for the first heating extraction is 80-88 °C.

[0012] Preferably, when performing the steaming, based on the total mass of the Cimicifuga racemosa extract, Paeonia lactiflora Pall. and Rehmannia glutinosa Libosch., the mass ratio of water to the total mass of the Cimicifuga racemosa extract, Paeonia lactiflora Pall. and Rehmannia glutinosa Libosch. is 7-8:1;

[0013] The time for the steaming is 3-4 h.

[0014] Preferably, when performing the second heating extraction, based on the total mass of Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC. and Peucedanum praeruptorum Dunn, the mass ratio of water to the total mass of Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC. and Peucedanum praeruptorum Dunn is 14-16:1;

[0015] The time for the second heating extraction is 2-3 h; the temperature for the second heating extraction is 75-82 °C.

[0016] The present invention provides an application of the composition described in the above technical solution in the preparation of a product for improving complications caused by low female hormones.

[0017] Preferably, the complications include: female vaginal atrophy.

[0018] The present invention provides a drug for improving complications caused by low female hormones, and the drug includes: the composition described in the above technical solution.

[0019] Beneficial effects:

[0020] The present invention provides a composition for improving complications caused by low female hormones, and the composition includes: Actaea racemosa, Paeonia lactiflora Pall., Rehmannia glutinosa Libosch., Trifolium pratense, Platycodon grandiflorus, and Peucedanum praeruptorum Dunn. By combining Actaea racemosa and Trifolium pratense, the present invention can effectively regulate the estrogen level in the female body and relieve discomfort caused by hormonal disorders; Rehmannia glutinosa Libosch. and Paeonia lactiflora Pall. are beneficial to moisturize the skin while improving qi and blood; Peucedanum praeruptorum Dunn and Platycodon grandiflorus are beneficial to improve the immune capacity of the body, soothe the nerves and relieve anxiety. Therefore, the above composition can achieve the effect of comprehensive conditioning.

[0021] Based on the above technical advantages, the present invention provides a preparation method of the composition described in the above technical solution, and its application in products for improving complications caused by low female hormones. By adding the above composition to the corresponding products, it is beneficial to release its efficacy and achieve the effect of improving complications caused by low female hormones. Detailed implementation mode

[0022] To further illustrate the present invention, the following describes in detail the solution provided by the present invention in combination with embodiments, but they cannot be understood as limiting the protection scope of the present invention.

[0023] Embodiment 1

[0024] A preparation method of a composition for improving complications caused by low female hormones, the steps are as follows:

[0025] (1) By mass fraction, the composition includes: 25 parts of Actaea racemosa, 15 parts of Paeonia lactiflora Pall., 10 parts of Rehmannia glutinosa Libosch., 8 parts of Trifolium pratense, 8 parts of Platycodon grandiflorus, and 7 parts of Peucedanum praeruptorum Dunn.

[0026] (2) Mix the crushed Actaea racemosa with ethanol with a volume concentration of 78% in a ratio of 1:10 and then carry out heat extraction (the heat extraction time is 2 h; the heat extraction temperature is 88 °C) to obtain an Actaea racemosa extract;

[0027] Mix the Actaea racemosa extract with the crushed Paeonia lactiflora Pall. and the crushed Rehmannia glutinosa Libosch. to obtain mixture 1; mix mixture 1 with water in a mass ratio of 1:8 and then cook for 3 h, and after filtration, obtain a first filtrate; concentrate the first filtrate to obtain a first extract;

[0028] Mix the crushed Trifolium pratense, the crushed Platycodon grandiflorus, and the crushed Peucedanum praeruptorum Dunn to obtain mixture 2; mix mixture 2 with water in a mass ratio of 1:15 and then carry out heat extraction (the heat extraction time is 3 h; the heat extraction temperature is 75 °C) to obtain an extract; carry out vacuum concentration on the extract to obtain a mixed extract;

[0029] The mixed extract was dissolved with ethanol having a volume concentration of 90%, and after removing the precipitate and concentrating, a second extract was obtained; after mixing the first extract and the second extract, a composition for improving complications caused by low female hormones was obtained.

[0030] Example 2

[0031] A method for preparing a composition for improving complications caused by low female hormones, the steps are as follows:

[0032] (1) By mass, the composition includes: 20 parts of Actaea racemosa, 20 parts of Paeonia lactiflora, 10 parts of Rehmannia glutinosa, 10 parts of Trifolium pratense, 8 parts of Platycodon grandiflorum, and 7 parts of Peucedanum praeruptorum.

[0033] (2) The crushed Actaea racemosa was mixed with ethanol having a volume concentration of 78% at a ratio of 1:10 and then subjected to heat extraction (the heat extraction time was 2 h; the heat extraction temperature was 88 °C) to obtain an Actaea racemosa extract;

[0034] The Actaea racemosa extract was mixed with the crushed Paeonia lactiflora and the crushed Rehmannia glutinosa to obtain mixture 1; mixture 1 was mixed with water at a mass ratio of 1:8 and then steamed for 3 h, and after filtration, a first filtrate was obtained; after concentrating the first filtrate, a first extract was obtained;

[0035] The crushed Trifolium pratense, the crushed Platycodon grandiflorum, and the crushed Peucedanum praeruptorum were mixed to obtain mixture 2; mixture 2 was mixed with water at a mass ratio of 1:15 and then subjected to heat extraction (the heat extraction time was 3 h; the heat extraction temperature was 75 °C) to obtain an extract; the extract was concentrated under reduced pressure to obtain a mixed extract;

[0036] The mixed extract was dissolved with ethanol having a volume concentration of 90%, and after removing the precipitate and concentrating, a second extract was obtained; after mixing the first extract and the second extract, a composition for improving complications caused by low female hormones was obtained.

[0037] Comparative Example 1

[0038] A method for preparing a composition for improving complications caused by low female hormones, the steps are as follows:

[0039] (1) By mass, the composition includes: 50 parts of Actaea racemosa, 15 parts of Paeonia lactiflora, 10 parts of Rehmannia glutinosa, 20 parts of Trifolium pratense, 8 parts of Platycodon grandiflorum, and 7 parts of Peucedanum praeruptorum.

[0040] (2) The crushed Actaea racemosa was mixed with ethanol having a volume concentration of 78% at a ratio of 1:10 and then subjected to heat extraction (the heat extraction time was 2 h; the heat extraction temperature was 88 °C) to obtain an Actaea racemosa extract;

[0041] Mix the black cohosh extract with the pulverized white peony root and the pulverized rehmannia root to obtain Mixture 1; mix Mixture 1 with water at a mass ratio of 1:8 and cook for 3 h, and after filtration, obtain the first filtrate; concentrate the first filtrate to obtain the first extract paste.

[0042] Mix the pulverized red clover, the pulverized platycodon root, and the pulverized common hogfennel root to obtain Mixture 2; mix the said Mixture 2 with water at a mass ratio of 1:15 and perform heat extraction (the heat extraction time is 3 h; the heat extraction temperature is 75 °C) to obtain an extract; perform vacuum concentration on the said extract to obtain a mixed extract.

[0043] Dissolve the mixed extract with ethanol having a volume concentration of 90%, remove the precipitate and concentrate to obtain the second extract paste; mix the first extract paste and the second extract paste to obtain a composition for improving complications caused by low female hormones.

[0044] Comparative Example 2

[0045] A preparation method of a composition for improving complications caused by low female hormones, the steps are as follows:

[0046] (1) By mass, the composition includes: 25 parts of black cohosh, 15 parts of white peony root, 10 parts of rehmannia root, 5 parts of red clover, 8 parts of platycodon root, and 7 parts of common hogfennel root.

[0047] (2) Mix the pulverized black cohosh with ethanol having a volume concentration of 78% at a ratio of 1:10 and perform heat extraction (the heat extraction time is 2 h; the heat extraction temperature is 88 °C) to obtain the black cohosh extract.

[0048] Mix the black cohosh extract with the pulverized white peony root and the pulverized rehmannia root to obtain Mixture 1; mix Mixture 1 with water at a mass ratio of 1:8 and cook for 3 h, and after filtration, obtain the first filtrate; concentrate the first filtrate to obtain the first extract paste.

[0049] Mix the pulverized red clover, the pulverized platycodon root, and the pulverized common hogfennel root to obtain Mixture 2; mix the said Mixture 2 with water at a mass ratio of 1:15 and perform heat extraction (the heat extraction time is 3 h; the heat extraction temperature is 75 °C) to obtain an extract; perform vacuum concentration on the said extract to obtain a mixed extract.

[0050] Dissolve the mixed extract with ethanol having a volume concentration of 90%, remove the precipitate and concentrate to obtain the second extract paste; mix the first extract paste and the second extract paste to obtain a composition for improving complications caused by low female hormones.

[0051] Comparative Example 3

[0052] A preparation method of a composition for improving complications caused by low female hormones, the steps are as follows:

[0053] (1) By mass, the composition includes: 25 parts of black cohosh, 15 parts of white peony root, 10 parts of prepared rehmannia root, 8 parts of red clover, 8 parts of platycodon root, and 7 parts of purple coneflower root.

[0054] (2) Mix the crushed black cohosh with ethanol with a volume concentration of 78% at a ratio of 1:10 and then carry out heating extraction (the time of heat extraction is 2 h; the temperature of heating extraction is 88 °C) to obtain a black cohosh extract;

[0055] Mix the black cohosh extract with the crushed white peony root and the crushed prepared rehmannia root to obtain mixture 1; mix mixture 1 with water at a mass ratio of 1:8 and then steam for 3 h, and after filtration, obtain a first filtrate; concentrate the first filtrate to obtain a first extract;

[0056] Mix the crushed red clover, the crushed platycodon root, and the crushed purple coneflower root to obtain mixture 2; mix mixture 2 with water at a mass ratio of 1:15 and then carry out heating extraction (the time of heat extraction is 3 h; the temperature of heating extraction is 75 °C), and after filtration, obtain a second filtrate; concentrate the second filtrate to obtain a second extract; mix the first extract and the second extract to obtain a composition for improving complications caused by low female hormones.

[0057] Comparative Example 4

[0058] A preparation method of a composition for improving complications caused by low female hormones, the steps are as follows:

[0059] (1) By mass, the composition includes: 25 parts of black cohosh, 15 parts of white peony root, 10 parts of prepared rehmannia root, 8 parts of red clover, 8 parts of platycodon root, and 7 parts of purple coneflower root.

[0060] (2) Crush the raw materials in step (1) respectively, mix the crushed black cohosh, white peony root, prepared rehmannia root, red clover, platycodon root, and purple coneflower root to obtain a mixture; mix the mixture with water at a mass ratio of 1:10 and then steam for 3 h, and after filtration, obtain a filtrate; concentrate the filtrate to obtain an extract, which is the composition for improving complications caused by low female hormones.

[0061] Comparative Example 5

[0062] A preparation method of a composition for improving complications caused by low female hormones, the steps are as follows:

[0063] (1) By mass, the composition includes: 25 parts of black cohosh, 15 parts of white peony root, 10 parts of prepared rehmannia root, 8 parts of red clover, 8 parts of platycodon root, and 7 parts of purple coneflower root.

[0064] (2) Mix the crushed black cohosh with ethanol at a volume concentration of 95% in a ratio of 1:10 and then carry out heating extraction (the time of heat extraction is 2 h; the temperature of heating extraction is 88 °C) to obtain a black cohosh extract;

[0065] Mix the black cohosh extract with the crushed white peony root and the crushed rehmannia root to obtain mixture 1; Mix mixture 1 with water in a mass ratio of 1:8 and then cook for 3 h, and after filtration, obtain the first filtrate; Concentrate the first filtrate to obtain the first extract;

[0066] Mix the crushed red clover, the crushed platycodon root and the crushed purple-flowered peucedanum root to obtain mixture 2; Mix the mixture 2 with water in a mass ratio of 1:8 and then carry out heating extraction (the time of heat extraction is 3 h; the temperature of heating extraction is 75 °C) to obtain an extract; Carry out vacuum concentration on the extract to obtain a mixed extract;

[0067] Dissolve the mixed extract with ethanol at a volume concentration of 75%, remove the precipitate and concentrate to obtain the second extract; Mix the first extract and the second extract to obtain a composition for improving the complications caused by low female hormones.

[0068] Comparative Example 6

[0069] A preparation method of a composition for improving the complications caused by low female hormones, the steps are as follows:

[0070] (1) By mass, the composition includes: 25 parts of black cohosh, 10 parts of rehmannia root, 8 parts of red clover, and 7 parts of purple-flowered peucedanum root.

[0071] (2) Mix the crushed black cohosh with ethanol at a volume concentration of 78% in a ratio of 1:10 and then carry out heating extraction (the time of heat extraction is 2 h; the temperature of heating extraction is 88 °C) to obtain a black cohosh extract;

[0072] Mix the black cohosh extract and the crushed rehmannia root to obtain mixture 1; Mix mixture 1 with water in a mass ratio of 1:8 and then cook for 3 h, and after filtration, obtain the first filtrate; Concentrate the first filtrate to obtain the first extract;

[0073] Mix the crushed red clover and the crushed purple-flowered peucedanum root to obtain mixture 2; Mix the mixture 2 with water in a mass ratio of 1:15 and then carry out heating extraction (the time of heat extraction is 3 h; the temperature of heating extraction is 75 °C) to obtain an extract; Carry out vacuum concentration on the extract to obtain a mixed extract;

[0074] Dissolve the mixed extract with ethanol having a volume concentration of 90%, remove the precipitate and concentrate to obtain a second extract; after mixing the first extract and the second extract, obtain a composition for improving complications caused by low female hormones.

[0075] Comparative Example 7

[0076] A preparation method of a composition for improving complications caused by low female hormones, the steps are as follows:

[0077] (1) The composition is combined with black cohosh and red clover in a mass ratio of 1:1.

[0078] (2) Mix the crushed black cohosh with ethanol having a volume concentration of 78% in a ratio of 1:10 and then carry out heating extraction (the time of heat extraction is 2 h; the temperature of heating extraction is 88 °C) to obtain a black cohosh extract; after filtering the black cohosh extract, obtain a first filtrate; after concentrating the first filtrate, obtain a first extract;

[0079] Mix the crushed red clover with water in a mass ratio of 1:15 and then carry out heating extraction (the time of heat extraction is 3 h; the temperature of heating extraction is 75 °C) to obtain an extract; after concentration, obtain a second extract; after mixing the first extract and the second extract, obtain a composition for improving complications caused by low female hormones.

[0080] Application Example 1

[0081] Respectively take 0.5 g of the compositions prepared in Examples 1-2 and Comparative Examples 1-7, and completely dissolve them in water to obtain 0.5% solutions respectively;

[0082] Use healthy female rats with a body weight of 205-215 g as experimental subjects, raise the rats in the same environment, and construct an ovariectomized rat model, that is, remove the bilateral ovaries of the rats, take the liquid in the vagina of the rats, smear the microscopic examination, add 5% methylene blue staining, observe under the microscope, and record the rats with zero keratinized epithelial cells and a cleanliness of II or above in the vaginal smear for ten consecutive days as successful in modeling;

[0083] Select 80 successfully modeled rats, randomly divide them into 8 groups, with 10 rats in each group, and respectively administer the 0.5% solution; the gavage volume is 2 mL / 100 g, once a day, and use the treatment group that is gavaged with the same volume of clear water as the blank control; after 50 days of gavage, take blood, and measure the plasma β-endorphin content according to the method provided by the β-Endorphin (Rat) radioimmunoassay kit, and the results are shown in Table 1.

[0084] Table 1 β-Endorphin concentration in different treatments (unit: pg / mL)

[0085] Treatment β - endorphin concentration Example 1 572.31 Example 2 550.16 Comparative Example 1 420.14 Comparative Example 2 380.25 Comparative Example 3 350.17 Comparative Example 4 110.68 Comparative Example 5 275.85 Comparative Example 6 150.82 Comparative Example 7 130.67 Blank control 64.15

[0086] As can be seen from the data in Table 1, compared with Comparative Examples 1-7 and the blank control, the compositions provided in Examples 1 and 2 for improving the complications caused by low female hormones can significantly increase the content of β-endorphin in plasma, which also indicates that the compositions provided by the present invention can effectively relieve the complications caused by low female hormones.

[0087] Application Example 2

[0088] According to the experimental results of Application Example 1, 0.5 g of the compositions prepared in Example 1 and Comparative Example 1 were taken and completely dissolved in water to obtain 0.5% solutions respectively to verify the effects of different compositions on estrogen levels.

[0089] Thirty rats with successful modeling (the method for constructing the ovariectomized rat model was the same as that in Example 1) were randomly divided into 3 groups, with 10 rats in each group, and each group was intragastrically administered the 0.5% solution; the intragastric administration volume was 2 mL / 100 g, once a day, and the treatment group intragastrically administered the same volume of clear water was used as the blank control; another 10 rats without ovariectomy were used as the positive control. After being raised in the same environment for 3 months, blood was taken, and the content of serum estradiol was measured using a kit. The results are shown in Table 2.

[0090] Table 2 Content of serum estradiol in different treatments (unit: pg / mL)

[0091] Treatment Serum estradiol Example 1 118.71 Comparative Example 1 95.35 Positive control 66.56 Blank control 48.32

[0092] As can be seen from the data in Table 2, compared with Comparative Example 1, the blank control, and the positive control, the composition for improving the complications caused by low female hormones in Example 1 can significantly increase the content of serum estradiol, that is, the composition provided by the present invention can effectively regulate female hormone levels.

[0093] In summary, by combining Actaea racemosa, Paeonia lactiflora, Rehmannia glutinosa, Trifolium pratense, Platycodon grandiflorum, and Peucedanum praeruptorum, the composition of the present invention can achieve the purpose of improving female hormones, and further achieve the effect of improving or relieving the complications caused by low female hormones.

[0094] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all embodiments. People can also obtain other embodiments without creative efforts based on these embodiments, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A composition for improving complications caused by low female hormones, characterized in that, The composition comprises: Actaea racemosa, Paeonia lactiflora Pall., Rehmannia glutinosa Libosch., Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC., and Peucedanum praeruptorum Dunn.

2. The composition according to claim 1, wherein By mass parts, the composition comprises: 20 - 25 parts of Actaea racemosa, 15 - 20 parts of Paeonia lactiflora Pall., 10 - 15 parts of Rehmannia glutinosa Libosch., 8 - 10 parts of Trifolium pratense L., 8 - 10 parts of Platycodon grandiflorum (Jacq.) A. DC., and 7 - 8 parts of Peucedanum praeruptorum Dunn.

3. The composition according to claim 1, wherein By mass parts, the composition comprises: 25 parts of Actaea racemosa, 15 parts of Paeonia lactiflora Pall., 10 parts of Rehmannia glutinosa Libosch., 8 parts of Trifolium pratense L., 8 parts of Platycodon grandiflorum (Jacq.) A. DC., and 7 parts of Peucedanum praeruptorum Dunn.

4. A method for preparing the composition according to any one of claims 1-3, characterized in that, It includes the following steps: Mix Actaea racemosa with ethanol having a volume concentration of 78% - 85% and then perform the first heating extraction to obtain an Actaea racemosa extract; Mix the Actaea racemosa extract, Paeonia lactiflora Pall., Rehmannia glutinosa Libosch., and water and then perform cooking to obtain a first filtrate; Concentrate the first filtrate to obtain a first extract; Mix Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC., Peucedanum praeruptorum Dunn, and water and then successively perform the second heating extraction and vacuum concentration to obtain a mixed extract; Dissolve the mixed extract with ethanol having a volume concentration of 90% - 95%, remove the precipitate and concentrate to obtain a second extract; Mix the first extract and the second extract to obtain a composition for improving complications caused by low female hormones.

5. The preparation method according to claim 4, wherein When performing the first heating extraction, the volume ratio of Actaea racemosa to ethanol is 1:8 - 10; The time of the first heating extraction is 2 - 3 h; the temperature of the first heating extraction is 80 - 88 °C.

6. The preparation method according to claim 4, characterized in that, When performing cooking, based on the total mass of the Actaea racemosa extract, Paeonia lactiflora Pall., and Rehmannia glutinosa Libosch., the mass ratio of water to the total mass of the Actaea racemosa extract, Paeonia lactiflora Pall., and Rehmannia glutinosa Libosch. is 7 - 8:1; The time of the cooking is 3 - 4 h.

7. The preparation method according to claim 4, characterized in that, When performing the second heating extraction, based on the total mass of Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC., and Peucedanum praeruptorum Dunn, the mass ratio of water to the total mass of Trifolium pratense L., Platycodon grandiflorum (Jacq.) A. DC., and Peucedanum praeruptorum Dunn is 14 - 16:1; The time of the second heating extraction is 2 - 3 h; the temperature of the second heating extraction is 75 - 82 °C.

8. Use of the composition according to any one of claims 1 - 3 in the preparation of a product for improving complications caused by low female hormones.

9. The application according to claim 8, characterized in that, The complications include: female vaginal atrophy.

10. A drug for improving complications caused by low female hormones, characterized in that, The medicament comprises: the composition according to any one of claims 1 - 3.