Compound microbial fertilizer with root-promoting and seedling-strengthening effects and preparation method of compound microbial fertilizer

Abstract

The invention discloses a compound microbial fertilizer with root-promoting and seedling-strengthening effects and a preparation method thereof, and belongs to the technical field of microbial fertilizer preparation. Comprising the following steps: adding phanerochaete chrysosporium, azotobacter, bacillus mucilaginosus and bacillus megatherium into a TSB liquid culture medium, and culturing to obtain a fermented spore suspension; mixing platycodon grandiflorum, plant ash and purified water to obtain a base material; inoculating the fermentation spore suspension into a base material, and fermenting to obtain a base fertilizer; the preparation method comprises the following steps: adding pichia kudriavzevii, bacillus licheniformis and trichoderma harzianum into a TSB liquid culture medium, and culturing to obtain an antibacterial spore suspension; inoculating the antibacterial spore suspension into a base fertilizer, and fermenting to obtain a base fertilizer; mixing a monopotassium phosphate solution, a dipotassium phosphate solution and fetal calf serum to obtain a flora protection solution; mixing and drying the flora protection liquid and the base fertilizer to obtain the compound microbial fertilizer. The number of viable bacteria in the compound microbial fertilizer is increased, and then the root-promoting and seedling-strengthening effects of the compound microbial fertilizer are improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of compound microbial fertilizer preparation, and particularly relates to a compound fertilizer with the function of promoting root growth and strengthening seedlings and its preparation method. Background Art

[0002] Microorganisms are crucial for soil and crops, which can improve the efficiency of soil nutrient conversion. Applying compound microbial fertilizers is one of the main ways for growers to improve soil, effectively increasing soil fertility and being beneficial for plants to resist pests and diseases.

[0003] Compound microbial fertilizer is a kind of fertilizer mainly derived from plants and / or animals, and its main function is to be applied to the soil to provide plant nutrition. This fertilizer is processed from biological substances, animal and plant wastes, and plant residues, eliminating the toxic and harmful substances therein and being rich in a large number of beneficial substances, including various organic acids, peptides, and abundant nutrient elements such as nitrogen, phosphorus, and potassium. Organic compound microbial fertilizer can not only provide comprehensive nutrition for crops, but also has a long fertilizer efficiency, can increase and renew soil organic matter, promote the reproduction of microorganisms, and improve the physical and chemical properties and biological activities of the soil, and is an important nutrient for the production of green foods.

[0004] However, at present, the viable count in compound microbial fertilizers on the market is not stable, and it is easy to continuously decrease with the change of storage environment and the passage of time, thus greatly reducing the effect of compound microbial fertilizers.

[0005] Patent CN 116693351 A2 discloses an organic compound microbial fertilizer and its preparation method. It is characterized in that, by weight, the organic compound microbial fertilizer includes: 120 of walnut pruning branches, 20 of plant ash, 24 of lignocellulose, 3 of soil-inhabiting bacteria, and 900 of water. The selenium-rich nutritional fortifier produced by the present invention can be used to produce various selenium-rich feeds and selenium-rich fertilizers, and has the advantages of convenient use, safety and reliability, good effect, low cost, and easy popularization and application. And it effectively solves the technical problem that the organic compound microbial fertilizer cannot be evaluated. Through the evaluation method for the organic compound microbial fertilizer, it is possible to comprehensively evaluate the organic compound microbial fertilizer depending on multiple indicators in multiple dimensions, and the evaluation result of the organic compound microbial fertilizer has a considerable degree of reliability, providing a strong reference basis for the performance improvement of the organic compound microbial fertilizer.

[0006] Although the above patent has optimized the effects of compound microbial fertilizers in various aspects, including the above patent, the viable count in compound microbial fertilizers on the market is not stable. As one of the most important parameters affecting the effect of compound microbial fertilizers, the viable count is easy to continuously decrease with the change of storage environment and the passage of time, thus greatly reducing the effect of compound microbial fertilizers.

[0007] Therefore, researching a compound microbial fertilizer with storage resistance and the function of promoting root growth and strengthening seedlings has broad application prospects. Summary of the Invention

[0008] In view of the deficiencies of the prior art, in the present invention, Phanerochaete chrysosporium, Azotobacter, Bacillus mucilaginosus and Bacillus megaterium are added to 50 ml of TSB liquid medium for fermentation, and the petri dish is rinsed with sterile water to obtain a fermented spore suspension; Platycodon grandiflorum, plant ash and purified water are mixed to obtain a base material. The fermented spore suspension is inoculated into the base material for fermentation to obtain base fertilizer. Kudriavzevia pseudotropicalis, Bacillus licheniformis and Trichoderma harzianum are added to 50 ml of TSB liquid medium for fermentation, and the petri dish is rinsed with sterile water to obtain an antibacterial spore suspension. The antibacterial spore suspension is inoculated into the base fertilizer for fermentation to obtain basal fertilizer. A potassium dihydrogen phosphate solution, a dipotassium hydrogen phosphate solution and fetal bovine serum are mixed to obtain a flora protection solution. The flora protection solution and the basal fertilizer are mixed and then dried to obtain the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings, thus solving the technical problems proposed in the background art. Specifically, the technical solution of the present invention includes the following contents: A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings, the preparation method comprising the following steps Phanerochaete chrysosporium, Azotobacter, Bacillus mucilaginosus and Bacillus megaterium are mixed to obtain a fermentation inoculant; The fermentation inoculant is inoculated into 50 ml of TSB liquid medium and pre-cultured for 2 days to obtain a fermented spore suspension; Platycodon grandiflorum, plant ash and purified water are mixed to obtain a base material; The fermented spore suspension is inoculated into the base material for fermentation to obtain base fertilizer; Kudriavzevia pseudotropicalis, Bacillus licheniformis and Trichoderma harzianum are mixed to obtain an antibacterial agent; The antibacterial agent is inoculated into 50 ml of TSB liquid medium and pre-cultured for 1 day to obtain an antibacterial spore suspension; The antibacterial spore suspension is inoculated into the base fertilizer for fermentation to obtain basal fertilizer; A potassium dihydrogen phosphate solution, a dipotassium hydrogen phosphate solution and fetal bovine serum are mixed to obtain a flora protection solution; The flora protection solution and the basal fertilizer are mixed and then dried to obtain a compound microbial fertilizer.

[0009] Furthermore, the mass ratio of Phanerochaete chrysosporium: Azotobacter: Bacillus mucilaginosus: Bacillus megaterium is 3:1:1:1. The functions of using Phanerochaete chrysosporium, Azotobacter, Bacillus mucilaginosus and Bacillus megaterium are as follows: they can effectively decompose lignocellulose and fix elements such as nitrogen, phosphorus and potassium to provide nutrients for plants.

[0010] Further, the process of inoculating the fermentation bacterial agent into 50 ml of TSB liquid medium for pre-cultivation includes inoculating the fermentation bacterial agent into 50 ml of TSB liquid medium and pre-cultivating it for 2 days at a temperature of 30 °C to obtain a fermentation spore suspension.

[0011] Further, the mass ratio of platycodon grandiflorum: plant ash: purified water is 4: 2-3: 14. The functions of using platycodon grandiflorum and plant ash are as follows: the hollow tubular structure of platycodon grandiflorum is conducive to ventilation, enhancing aerobic fermentation of the bacterial agent, and plant ash is rich in a large number of nutrients.

[0012] Further, the process of inoculating the fermentation spore suspension into the base material for fermentation includes mixing the fermentation spore suspension and the base material evenly, spreading them flat on a glass plate with a thickness of 3 cm, and maintaining the water content above 30% in an environment at a temperature of 30 °C, and fermenting for 15 days.

[0013] Further, the mass ratio of Pichia kudriavzevii: Bacillus licheniformis: Trichoderma harzianum is 1: 1: 1. The functions of using Pichia kudriavzevii, Bacillus licheniformis and Trichoderma harzianum are as follows: it can improve the ability of plants to resist diseases and pests.

[0014] Further, the process of inoculating the anti-pathogenic bacterial agent into 50 ml of TSB liquid medium for pre-cultivation includes inoculating the anti-pathogenic bacterial agent into 50 ml of TSB liquid medium and pre-cultivating it for 1 day at a temperature of 28 °C and a humidity of 60% to obtain an anti-disease spore suspension.

[0015] Further, the process of inoculating the anti-disease spore suspension into the base fertilizer for fermentation includes mixing the anti-disease spore suspension and the base fertilizer evenly, spreading them flat on a glass plate again with a thickness of 5 cm, and maintaining the water content above 30% in an environment at 28 °C, and fermenting until the viable bacteria count reaches 1×10 9 cfu / g.

[0016] Further, the potassium dihydrogen phosphate solution is obtained by mixing potassium dihydrogen phosphate and purified water according to a mass ratio of 136: 1000, and the dipotassium hydrogen phosphate solution is obtained by mixing dipotassium hydrogen phosphate and purified water according to a mass ratio of 174: 1000.

[0017] Further, the mass ratio of the potassium dihydrogen phosphate solution: the dipotassium hydrogen phosphate solution: fetal bovine serum is 1: 1: 3-8. The functions of using the potassium dihydrogen phosphate solution, the dipotassium hydrogen phosphate solution and fetal bovine serum are as follows: to provide a buffer barrier for the bacterial community.

[0018] Further, the mass ratio of the bacterial community protection solution: base fertilizer is 1-2: 50.

[0019] A compound microbial fertilizer with the function of promoting root growth and strengthening seedlings prepared by the preparation method of a compound microbial fertilizer.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) In the present invention, a fermentation bacterium agent beneficial to nutrient production is obtained through the combination of Phanerochaete chrysosporium, Azotobacter, Bacillus mucilaginosus, and Bacillus megaterium, and pre-cultured. Then, through the appropriate ratio of platycodon root, plant ash, and purified water, a fermentation base material is obtained. Then, the fermentation bacterium agent is mixed with the base material for fermentation to release the nutrients required by plants to obtain base fertilizer.

[0021] (2) The combination of Pichia kudriavzevii, Bacillus licheniformis, and Trichoderma harzianum is used to obtain an antibacterial agent beneficial for plants to resist diseases and pests. Then, the antibacterial agent is mixed and fermented with the base fertilizer to make the viable count reach 1×10 9 cfu / g or more to obtain base manure, realizing the effect of promoting root growth and strengthening seedlings of the compound bacterium agent.

[0022] (3) A special bacterial community protection solution is obtained by combining potassium dihydrogen phosphate solution, dipotassium hydrogen phosphate solution, and fetal bovine serum. Finally, the bacterial community protection solution and the base manure are mixed and dried to obtain a compound bacterium agent. This bacterial community protection solution provides a buffer barrier for the bacterial community in the compound bacterium agent, thereby reducing the influence of the storage environment and storage time on the activity of the bacterial community. Specific embodiments

[0023] Next, through the examples of the present invention, the technical solutions of the present invention will be clearly and completely described. Obviously, the described examples are only a part of the examples of the present invention, rather than all the examples. Based on the examples in the present invention, all other examples obtained by those skilled in the art without creative efforts belong to the scope of protection of the present invention.

[0024] Unless otherwise specified, the raw materials and reagents used in the present invention below are all commercially available products, or can be prepared by known methods.

[0025] Phanerochaete chrysosporium CICC 40719, Azotobacter CICC 20837, Bacillus mucilaginosus CICC23575, Bacillus megaterium CICC 20605, Pichia kudriavzevii CICC 33192, Trichoderma harzianum CICC13056, and Bacillus licheniformis CICC 10095 were purchased from the China Center for Industrial Culture Collection.

[0026] Potassium dihydrogen phosphate and dipotassium hydrogen phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0027] Fetal bovine serum was purchased from Thermo Fisher Scientific (China) Co., Ltd.

[0028] Preparation Example 1: Preparation of the fermentation spore suspension, including the following process: Mix three tubes of Phanerochaete chrysosporium CICC 40719 with one tube each of Azotobacter CICC 20837, Bacillus mucilaginosus CICC 23575, and Bacillus megaterium CICC 20605 to obtain a fermentation inoculum. Inoculate the fermentation inoculum into a test tube containing 50 ml of TSB liquid medium, and place it in a constant temperature incubator set at 30°C for 2 days to obtain a fermentation spore suspension.

[0029] Preparation Example 2: Preparation of the base material, including the following process: Add 1400 g of purified water, 400 g of Platycodon grandiflorum granules, and 200 g of plant ash to a beaker and stir evenly to obtain the base material.

[0030] Preparation Example 3: Preparation of the disease-resistant spore suspension, including the following process: Mix one tube each of Pichia kudriavzevii CICC 33192, Trichoderma harzianum CICC 13056, and Bacillus licheniformis CICC 10095 to obtain an antibacterial agent. Inoculate the antibacterial agent into a test tube containing 50 ml of TSB liquid medium, and place it in a mold incubator set at 30°C and 60% humidity for 1 day to obtain a disease-resistant spore suspension.

[0031] Preparation Example 4: Preparation of the flora protection solution, including the following process: Add 60 ml of purified water to a volumetric flask, then add 13.6 g of potassium dihydrogen phosphate and stir evenly, and then make up the volume to 100 ml to obtain 100 ml of potassium dihydrogen phosphate solution. Add 60 ml of purified water to another volumetric flask, then add 17.4 g of dipotassium hydrogen phosphate and stir evenly, and then make up the volume to 100 ml to obtain 100 ml of dipotassium hydrogen phosphate solution. Take 2 g of the potassium dihydrogen phosphate solution, 2 g of the dipotassium hydrogen phosphate solution, and 16 g of fetal bovine serum and put them into a beaker and stir evenly to obtain the flora protection solution.

[0032] Preparation Example 5: Preparation of the base material, including the following process: Add 1400 g of purified water, 400 g of Platycodon grandiflorum granules, and 300 g of plant ash to a beaker and stir evenly to obtain the base material.

[0033] Preparation Example 6: Preparation of the flora protection solution, including the following process: Add 60 ml of purified water to a volumetric flask, then add 13.6 g of potassium dihydrogen phosphate. After stirring evenly, make up the volume to 100 ml to obtain a 100 ml potassium dihydrogen phosphate solution. Add 60 ml of purified water to another volumetric flask, then add 17.4 g of dipotassium hydrogen phosphate. After stirring evenly, make up the volume to 100 ml to obtain a 100 ml dipotassium hydrogen phosphate solution. Take 4 g of the potassium dihydrogen phosphate solution, 4 g of the dipotassium hydrogen phosphate solution, and 12 g of fetal bovine serum and put them into a beaker. Stir evenly to obtain a flora protection solution.

[0034] Preparation Example 7: Preparation of the fermented spore suspension, including the following process: Inoculate six tubes of Phanerochaete chrysosporium CICC 40719 into test tubes containing 50 ml of TSB liquid medium, and place them in a constant temperature incubator at a set temperature of 30 °C for 2 days to obtain a fermented spore suspension.

[0035] Preparation Example 8: Preparation of the flora protection solution, including the following process: Add 60 ml of purified water to a volumetric flask, then add 13.6 g of potassium dihydrogen phosphate. After stirring evenly, make up the volume to 100 ml to obtain a 100 ml potassium dihydrogen phosphate solution. Add 60 ml of purified water to another volumetric flask, then add 17.4 g of dipotassium hydrogen phosphate. After stirring evenly, make up the volume to 100 ml to obtain a 100 ml dipotassium hydrogen phosphate solution. Take 10 g of the potassium dihydrogen phosphate solution and 10 g of the dipotassium hydrogen phosphate solution and put them into a beaker. Stir evenly to obtain a flora protection solution.

[0036] Example 1:

[0037] A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings, specifically including the following process: Put the fermented spore suspension of Preparation Example 1 and the base fertilizer of Preparation Example 2 into a beaker, use a stirrer to stir at a speed of 20 r / m for 5 min, and then pour it on a glass plate to form a flat plate with a thickness of 3 cm. Place this flat plate in a constant temperature incubator at a set temperature of 30 °C for fermentation for 15 days. During this period, add water every day to keep the water content above 30% to obtain the base fertilizer. Put the disease-resistant spore suspension of Preparation Example 3 and the base fertilizer into a beaker, use a stirrer to stir at a speed of 20 r / m for 5 min, and then pour it on a glass plate to form a flat plate with a thickness of 5 cm. Place this flat plate in a constant temperature incubator at a set temperature of 28 °C for fermentation. During this period, add water every day to keep the water content above 30% and detect the viable bacteria count until the viable bacteria count reaches 1×10 9 cfu / g to terminate the fermentation to obtain the base fertilizer. Take 1000 g of the base fertilizer and the flora protection solution of Preparation Example 4 and put them into a beaker. Use a stirrer to stir at a speed of 20 r / m for 5 min, and then put it into an oven at a set temperature of 35 °C for baking for 12 h to obtain the compound microbial fertilizer.

[0038] Example 2:

[0039] A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings specifically includes the following process: Put the fermentation spore suspension of Preparation Example 1 and the base fertilizer of Preparation Example 5 into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, and then pour it on a glass plate to form a flat plate with a thickness of 3 cm. Put this flat plate into a constant temperature incubator and set the temperature at 30 °C for fermentation for 15 days. During this period, add water every day to keep the water content above 30% to obtain the base fertilizer. Put the disease-resistant spore suspension of Preparation Example 3 and the base fertilizer into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, and then pour it on a glass plate to form a flat plate with a thickness of 5 cm. Put this flat plate into a constant temperature incubator and set the temperature at 28 °C for fermentation. During this period, add water every day to keep the water content above 30% and detect the viable bacteria count until the viable bacteria count reaches 1×10 9 cfu / g to terminate fermentation to obtain the base fertilizer. Take 1000 g of the base fertilizer and the bacterial community protection liquid of Preparation Example 4 and put them into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, and then put them into an oven and set the temperature at 35 °C for baking for 12 h to obtain the compound microbial fertilizer.

[0040] Example 3:

[0041] A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings specifically includes the following process: Put the fermentation spore suspension of Preparation Example 1 and the base fertilizer of Preparation Example 2 into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, and then pour it on a glass plate to form a flat plate with a thickness of 3 cm. Put this flat plate into a constant temperature incubator and set the temperature at 30 °C for fermentation for 15 days. During this period, add water every day to keep the water content above 30% to obtain the base fertilizer. Put the disease-resistant spore suspension of Preparation Example 3 and the base fertilizer into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, and then pour it on a glass plate to form a flat plate with a thickness of 5 cm. Put this flat plate into a constant temperature incubator and set the temperature at 28 °C for fermentation. During this period, add water every day to keep the water content above 30% and detect the viable bacteria count until the viable bacteria count reaches 1×10 9 cfu / g to terminate fermentation to obtain the base fertilizer. Take 1000 g of the base fertilizer and the bacterial community protection liquid of Preparation Example 6 and put them into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, and then put them into an oven and set the temperature at 35 °C for baking for 12 h to obtain the compound microbial fertilizer.

[0042] Example 4:

[0043] A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings specifically includes the following process: Put the fermented spore suspension of Preparation Example 1 and the base fertilizer of Preparation Example 5 into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, then pour it onto a glass plate and spread it into a plate with a thickness of 3 cm. Put this plate into a constant temperature incubator and set the temperature at 30 °C for fermentation for 15 days. During this period, add water every day to keep the water content above 30% to obtain the base fertilizer. Put the disease-resistant spore suspension of Preparation Example 3 and the base fertilizer into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, then pour it onto a glass plate and spread it into a plate with a thickness of 5 cm. Put this plate into a constant temperature incubator and set the temperature at 28 °C for fermentation. During this period, add water every day to keep the water content above 30% and detect the viable bacteria count until the viable bacteria count reaches 1×10 9 cfu / g to terminate the fermentation and obtain the base fertilizer. Take 1000 g of the base fertilizer and the microbial community protection solution of Preparation Example 6 and put them into a beaker. Stir with a stirrer at a speed of 20 r / m for 5 min, and then put it into an oven and set the temperature at 35 °C for baking for 12 h to obtain the compound microbial fertilizer.

[0044] Example 5:

[0045] A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings specifically includes the following process: Put the fermented spore suspension of Preparation Example 1 and the base fertilizer of Preparation Example 5 into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, then pour it onto a glass plate and spread it into a plate with a thickness of 3 cm. Put this plate into a constant temperature incubator and set the temperature at 30 °C for fermentation for 15 days. During this period, add water every day to keep the water content above 30% to obtain the base fertilizer. Put the disease-resistant spore suspension of Preparation Example 3 and the base fertilizer into a beaker, stir with a stirrer at a speed of 20 r / m for 5 min, then pour it onto a glass plate and spread it into a plate with a thickness of 5 cm. Put this plate into a constant temperature incubator and set the temperature at 28 °C for fermentation. During this period, add water every day to keep the water content above 30% and detect the viable bacteria count until the viable bacteria count reaches 1×10 9 cfu / g to terminate the fermentation and obtain the base fertilizer. Take 500 g of the base fertilizer and the microbial community protection solution of Preparation Example 4 and put them into a beaker. Stir with a stirrer at a speed of 20 r / m for 5 min, and then put it into an oven and set the temperature at 35 °C for baking for 12 h to obtain the compound microbial fertilizer.

[0046] Comparative Example 1: A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings specifically includes the following process: Replace the fermented spore suspension of Preparation Example 1 in Example 5 with the fermented spore suspension of Preparation Example 7, and keep the other conditions the same as those in Example 5.

[0047] Comparative Example 2: A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings specifically includes the following process: The bacterial colony protection solution of Preparation Example 6 in Example 5 was replaced with the bacterial colony protection solution of Preparation Example 8, and the other conditions remained the same as in Example 5.

[0048] Comparative Example 3: A method for preparing a composite microbial fertilizer having the function of promoting root growth and strengthening seedlings, specifically comprising the following steps: The flora protection solution of Preparation Example 6 in Example 5 was replaced with 20 g of fetal bovine serum, and the other conditions remained the same as in Example 5.

[0049] Comparative Example 4: A method for preparing a composite microbial fertilizer having the function of promoting root growth and strengthening seedlings, specifically comprising the following steps: The fermented spore suspension of Preparation Example 1 and the base fertilizer of Preparation Example 5 were placed in a beaker, stirred at a speed of 20r / m for 5min with a stirrer, and then poured onto a glass plate to spread a 3cm thick flat plate. The flat plate was placed in a constant temperature incubator and set to 30°C for fermentation for 15 days. During this period, water was added every day to keep the water content above 30% to obtain the base fertilizer. The disease-resistant spore suspension and base fertilizer of Preparation Example 3 were placed in a beaker, stirred at a speed of 20r / m for 5min with a stirrer, and then poured onto a glass plate to spread a 5cm thick flat plate. The flat plate was placed in a constant temperature incubator and set to 28°C for fermentation. During this period, water was added every day to keep the water content above 30% and the number of viable bacteria was detected until the number of viable bacteria reached 1×10 9 cfu / g, and the fermentation was terminated to obtain the base fertilizer. 1000g of the base fertilizer was placed in an oven at 35°C and baked for 12 hours to obtain the composite microbial fertilizer.

[0050] The composite microbial fertilizers obtained in Examples 1 to 5 and Comparative Examples 1 to 4 were stored in a cool and ventilated environment at 25°C for 180 days, and then fully mixed with water and allowed to stand. The upper layer of liquid was centrifuged, and the supernatant after centrifugation was diluted. After dilution, a blood cell count was performed to obtain the number of viable bacteria as shown in the following table:

[0051] The same batch of tomato seeds were evenly sown in the same soil environment. After 60 days of planting, 100 well-grown tomato vines were selected for single plant isolation and randomly divided into 10 groups. The composite microbial fertilizers obtained in Examples 1 to 5 and Comparative Examples 1 to 4 were stored in a cool and ventilated environment at 25°C for 180 days, and then 9 groups of tomato vines were fertilized, with each tomato vine being fertilized 10g for three consecutive times, each with an interval of 40 days. The remaining group was used as a control group and no fertilizer was applied. After a total of 180 days of planting, all tomato vines were dug out, and after removing the soil on the rhizomes, all tomato vines in each group were weighed. The total weight of the tomato vines in the control group was taken as 1, and the weight ratios of the tomato vines in each group were as follows:

[0052] It can be seen from the above two sets of data that: (1) The compound microbial fertilizer in Examples 1-5 still has a high viable count after long-term storage, and has a significant effect on promoting root growth and strengthening seedlings of tomato vines.

[0053] (2) It can be seen from Comparative Example 1 that using a single Phanerochaete chrysosporium as the fermentation inoculant basically does not affect the viable count in the compound microbial fertilizer, but it cannot fully release the nutrients in the compound microbial fertilizer, which is not conducive to the absorption of tomatoes, resulting in an insignificant effect on promoting root growth and strengthening seedlings of tomato vines.

[0054] (3) It can be seen from Comparative Example 2 that using a buffer solution group composed of potassium dihydrogen phosphate solution and dipotassium hydrogen phosphate solution alone as the bacterial community protection solution, although it has a certain protective effect on the viable count, it is obviously not as effective as the bacterial community protection solution in Examples 1-5, and ultimately leads to a great reduction in the effect of promoting root growth and strengthening seedlings of tomato vines.

[0055] (4) It can be seen from Comparative Example 3 that using fetal bovine serum alone as the bacterial community protection solution, although it has a certain protective effect on the viable count, it is also not as effective as the bacterial community protection solution in Examples 1-5, and ultimately leads to a great reduction in the effect of promoting root growth and strengthening seedlings of tomato vines.

[0056] (5) It can be seen from Comparative Example 4 that if the compound microbial fertilizer does not add a bacterial community protection solution, a large number of viable counts will indeed decrease during long-term storage, ultimately resulting in poor effects on promoting root growth and strengthening seedlings of tomato vines.

[0057] The above-described embodiments have detailed the technical solutions and beneficial effects of the present invention. It should be understood that the above are only specific embodiments of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed.

Claims

1. A preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings, characterized in that, The preparation method comprises the following steps: Mixing Phanerochaete chrysosporium, Azotobacter, Bacillus mucilaginosus and Bacillus megaterium to obtain a fermentation inoculant; Inoculating the fermentation inoculant into 50 ml of TSB liquid medium and pre-culturing for 2 days to obtain a fermentation spore suspension; Mixing platycodon root, plant ash and purified water to obtain a base material; Inoculating the fermentation spore suspension into the base material and fermenting to obtain a base fertilizer; Mixing Pichia kudriavzevii, Bacillus licheniformis and Trichoderma harzianum to obtain an anti-pathogen agent; Inoculating the anti-pathogen agent into 50 ml of TSB liquid medium and pre-culturing for 1 day to obtain an anti-disease spore suspension; Inoculating the anti-disease spore suspension into the base fertilizer and fermenting to obtain a basal fertilizer; Mixing potassium dihydrogen phosphate solution, dipotassium hydrogen phosphate solution and fetal bovine serum to obtain a flora protection solution; Mixing the flora protection solution and the basal fertilizer and drying to obtain a compound microbial fertilizer.

2. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The mass ratio of Phanerochaete chrysosporium: Azotobacter: Bacillus mucilaginosus: Bacillus megaterium is 3:1:1:

1.

3. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The mass ratio of platycodon root: plant ash: purified water is 4:2-3:

14.

4. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The process of inoculating the fermentation spore suspension into the base material for fermentation includes mixing the fermentation spore suspension and the base material evenly, spreading them on a glass plate with a thickness of 3 cm, and maintaining the water content above 30% in an environment at 30 °C and fermenting for 15 days.

5. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The mass ratio of Pichia kudriavzevii: Bacillus licheniformis: Trichoderma harzianum is 1:1:

1.

6. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The process of inoculating the disease-resistant spore suspension into the base fertilizer for fermentation includes mixing the disease-resistant spore suspension and the base fertilizer evenly, then spreading them again on a glass plate to a thickness of 5 cm and maintaining the water content above 30% in an environment of 28 °C, and fermenting until the viable count reaches 1×10 9 cfu / g.

7. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that The potassium dihydrogen phosphate solution is obtained by mixing potassium dihydrogen phosphate and purified water according to a mass ratio of 136:1000, and the dipotassium hydrogen phosphate solution is obtained by mixing dipotassium hydrogen phosphate and purified water according to a mass ratio of 174:1000.

8. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The mass ratio of potassium dihydrogen phosphate solution: dipotassium hydrogen phosphate solution: fetal bovine serum is 1:1:3-8.

9. The preparation method of the compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to claim 1, characterized in that, The mass ratio of the flora protection solution: basal fertilizer is 1-2:

50.

10. A compound microbial fertilizer prepared by the preparation method of a compound microbial fertilizer with the function of promoting root growth and strengthening seedlings according to any one of claims 1-9.

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