Botanical small molecule peptide composition for hygiene and disinfection and application of botanical small molecule peptide composition

Abstract

The invention focuses on the fields of biotechnology and sanitary disinfection, and successfully separates five brand-new small molecule peptides (small molecule peptides K, L, M, N and O) from herba violae and spica prunellae. According to the innovative extraction method from raw material pretreatment, mixed extraction to multi-step purification, a disinfection action mechanism of destroying pathogen cell membranes, interfering energy metabolism and inhibiting biosynthesis by small molecular peptides is defined. The small molecule peptides show the advantages of efficient disinfection activity, high safety, good stability and difficult generation of drug resistance. On the basis, a series of products such as hygienic disinfection spray, wet tissues and hand sanitizer are developed, the market application prospect is wide, remarkable economic and social benefits can be brought, and a brand-new and high-quality solution is provided for the field of hygienic disinfection.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology and sanitation and disinfection, and in particular relates to a plant-derived sanitation and disinfection small molecule peptide combination. Background Art

[0002] In today's society, sanitation and disinfection play an irreplaceable and key role in maintaining public health and preventing the spread of diseases. From medical treatment facilities such as hospitals and clinics, to crowded public education areas such as schools and kindergartens, to daily family environments, sanitation and disinfection run through every corner of people's lives. However, there are many thorny problems in the sanitation and disinfection products and technologies currently on the market that need to be solved urgently. Chemical disinfectants have long dominated the field of sanitation and disinfection. Take chlorine-containing disinfectants as an example. Sodium hypochlorite, chlorine dioxide, etc., with their strong oxidizing ability, can quickly kill a variety of pathogens. However, this type of disinfectant is accompanied by a strong irritating odor, which can easily irritate the human respiratory tract during use, causing uncomfortable symptoms such as coughing and asthma. Long-term contact may even damage the respiratory mucosa. For the skin, chlorine-containing disinfectants are corrosive and can cause dryness, itching, redness and swelling of the skin. What is more serious is that after chlorine-containing disinfectants are used in the environment, they may generate harmful substances such as organic chlorides. These substances have potential carcinogenic and teratogenic risks, causing long-term pollution and damage to the ecological environment. Alcohol disinfectants, such as ethanol and isopropanol, are widely used in daily life because of their high volatility, rapid action and relative safety. However, the flammability of alcohol disinfectants makes them a major safety hazard during storage and use, especially in high temperature environments or near open flames, which can easily cause fire accidents. Moreover, alcohol disinfectants are not very effective in killing certain viruses (such as non-enveloped viruses) and spores, making it difficult to meet the effective disinfection needs of these pathogens. As an emerging research direction in the field of sanitation and disinfection, natural plant extracts have attracted much attention in recent years. Plants are rich in a variety of bioactive ingredients such as flavonoids, terpenes, alkaloids, etc., and have certain antibacterial and antiviral effects. However, the existing natural plant extracts have many limitations in practical applications. On the one hand, the composition of plant extracts is complex, and the quality stability between different batches is difficult to guarantee, resulting in uneven disinfection effects. On the other hand, the mechanism of action of the active ingredients in plant extracts is unclear, making it difficult to carry out targeted optimization and improvement. In addition, plant extracts have poor solubility in water, which limits their application in some water-based disinfection products. As a class of bioactive compounds, small molecule peptides play a variety of important physiological functions in organisms. In recent years, the research on small molecule peptides in the fields of antibacterial and antiviral has gradually increased, and they have the advantages of high activity, strong specificity, and good safety. However, at present, relatively few small molecule peptide products have been developed for the field of health disinfection, and the existing products have problems such as high cost and poor stability. Therefore, it is of great practical significance and market demand to develop a highly efficient, safe, stable and low-cost small molecule peptide for the field of health disinfection. Summary of the Invention

[0003] A combination of small molecule peptides for plant-derived health disinfection, comprising the following 5 small molecule peptides: - Small molecule peptide P: Gly-Leu-Gln-His-Asp-Pro-Lys-Cys-Ile-Ser-Tyr-Asn-Glu-Val-Thr (GLQHDPRCISYNEVT); - Small molecule peptide Q: His-Ala-Ser-Cys-Glu-Trp-Pro-Gly-Lys-Val-Tyr-Asp-Cys-Leu-Met (HASCEWPGKVYDCLM); - Small molecule peptide R: Ala-Cys-His-Lys-Asp-Pro-Ile-Leu-Ser-Tyr-Asn-Glu-Val-Met-Thr (ACHKDPI LSYNEVMT); - Small molecule peptide S: Thr-Glu-Asp-Cys-His-Pro-Arg-Gly-Ile-Val-Leu-Ser-Phe-Tyr-Lys (TEDCHPR GIVLSFYK); - Small molecule peptide T: Val-Pro-Cys-Lys-Trp-Asp-His-Ile-Gly-Leu-Met-Thr-Phe-Asn-Glu (VPCKW DHI GLMTFNE).

[0004] Furthermore, the extraction method of the small molecule peptide combination includes the following steps: - Raw material pretreatment: Select the whole herb of Andrographis paniculata and the rhizome of Isatis indigotica, wash, dry, and crush them into powder; - Mixed extraction: Mix the Andrographis paniculata and Isatis indigotica powder in a mass ratio of 3:2, add 10 times the volume of 70% ethanol - water mixed solution, and perform ultrasonic - assisted reflux extraction at 60°C for 3 times, 2 hours each time. Combine the extraction solutions and concentrate under reduced pressure; - Macroporous resin adsorption and separation: Pass the concentrated solution through an AB - 8 macroporous adsorption resin column, and elute successively with deionized water and 45% ethanol solution, and collect the eluate; - Enzymatic reaction: Adjust the pH of the eluate to 7.5, add a mixed enzyme solution of trypsin and neutral protease (mass ratio 3:2, total addition amount is 3% of the mass of the eluate), and perform oscillating enzymatic hydrolysis at 45°C for 6 hours; - Ultrafiltration purification: Ultrafilter the enzymatic hydrolysate through an ultrafiltration membrane with a molecular weight cut - off of 3000Da, and collect the permeate; - Ion - exchange chromatography: Load the permeate onto a DEAE - Sepharose Fast Flow weak anion - exchange resin column, first rinse with 0.03M Tris - HCl buffer solution (pH 7.5), and then elute with a gradient of 0 - 0.5M sodium chloride solution, and collect the elution peak with disinfection activity; - Gel filtration chromatography: Load the elution peak onto a Sephadex G - 25 gel column, elute with 0.1M phosphate buffer solution (pH 7.0), and collect the target small molecule peptide component; - Freeze - drying: Freeze - dry the collected small molecule peptide component to obtain small molecule peptide dry powder.

[0005] Furthermore, the application of the small molecule peptide combination in hygiene and disinfection products, where the hygiene and disinfection products include but are not limited to hygiene and disinfection sprays, hygiene and disinfection wet wipes, hygiene and disinfection hand sanitizers, and hygiene and disinfection gels.

[0006] Furthermore, for the said application, the formula of the hygiene and disinfection spray is 25 mg of small molecule peptide P, 6 mL of propylene glycol, 1.5 mL of polysorbate - 80, 0.15 g of propyl p - hydroxybenzoate per 100 mL, and purified water is added to 100 mL; - The formula of the hygiene and disinfection wet wipe is 30 mg of small molecule peptide Q, 8 g of glycerol, 2 mL of polyoxyethylene sorbitan monooleate (Tween - 80), 0.2 g of ethylparaben per 100 g, and purified water is added to 100 g; - The formula of the hygiene and disinfection hand sanitizer is 22 mg of small molecule peptide R, 14 g of sodium lauryl polyether sulfate, 6 g of cocamidopropyl betaine, 10 g of glycerol, an appropriate amount of citric acid (adjust the pH value to 7.0 - 7.5), an appropriate amount of fragrance per 100 g, and purified water is added to 100 g; - The formula of the hygiene and disinfection gel is 28 mg of small molecule peptide S, 1.5 g of carbomer 940, 1.2 g of triethanolamine, 7 g of propylene glycol, 0.18 g of methyl p - hydroxybenzoate per 100 g, and purified water is added to 100 g.

[0007] Furthermore, the small molecule peptide exerts a hygienic disinfection effect by disrupting the cell membrane structure of pathogens, interfering with the energy metabolism of pathogens, and inhibiting the biosynthesis of pathogens.

[0008] On the other hand, a hygienic disinfection product comprises the small molecule peptide combination as described above and a pharmaceutically acceptable carrier or excipient.

[0009] Furthermore, the product dosage form is liquid, semi-solid or solid.

[0010] Furthermore, for the hygienic disinfection product as described above, the liquid dosage form includes solution, suspension, emulsion; - the semi-solid dosage form includes gel, ointment; - the solid dosage form includes tablet, capsule, powder.

[0011] Furthermore, for the small molecule peptide combination, extraction method, application or hygienic disinfection product as described above, the small molecule peptide combination is used to kill or inhibit pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, influenza virus, respiratory syncytial virus, herpes simplex virus type 1, etc.

[0012] Furthermore, in the raw material pretreatment step, the origin, harvesting season, and growth environment of Andrographis paniculata and Isatis indigotica are comprehensively considered to ensure the stability and consistency of the raw material quality; in the mixed extraction step, an online monitoring device is used to monitor the extraction temperature, ultrasonic power, and extraction time in real time; in the macroporous resin adsorption and separation step, parameters such as the loading flow rate, eluent concentration, and elution volume are optimized through dynamic adsorption experiments; in the enzymatic hydrolysis reaction step, high performance liquid chromatography (HPLC) technology is used to monitor the changes in peptide segments during the enzymatic hydrolysis process in real time; in the ultrafiltration purification step, a membrane flux monitor is used to monitor the flux changes of the ultrafiltration membrane in real time; in the ion exchange chromatography step, efficient separation of small molecule peptides is achieved by optimizing the elution gradient, flow rate, and buffer composition; in the gel filtration chromatography step, the eluent flow rate is ensured to be stable and an automatic fraction collector is used to accurately collect the eluent; in the freeze-drying step, a vacuum freeze dryer is used and parameters such as the freezing temperature, vacuum degree, and drying time are monitored in real time..

[0013] Advantages of the invention: High - efficiency disinfection activity: The small - molecule peptides of the present invention have significant inhibitory and killing effects on a variety of common pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Candida albicans, and influenza virus. Good disinfection effects can be achieved at low concentrations (5 - 30 μg / mL), and the disinfection activity is superior to many traditional disinfectants and existing plant - derived disinfection products. Experimental data shows that at a concentration of 15 μg / mL, the inhibition zone diameter of small - molecule peptide P against Staphylococcus aureus can reach 18 mm, while the inhibition zone diameter of a traditional plant extract disinfectant at the same concentration is only 10 mm. Through a variety of microbial experimental models and disinfection effect evaluation methods, the high - efficiency disinfection activity of small - molecule peptides has been comprehensively verified. Good safety: The small - molecule peptides are derived from natural Andrographis paniculata and Isatis indigotica, and are purified through multiple steps without harmful chemical substances. Animal experiments show that high - dose use has no obvious effect on the physiological indicators of experimental animals, has no irritation to human skin and mucous membranes, and has no risk of allergy, and can be safely used in the field of health disinfection. A series of safety evaluation experiments such as acute toxicity test, sub - chronic toxicity test, skin irritation test, and eye irritation test have fully proved the safety of small - molecule peptides. Excellent stability: The small - molecule peptides can still maintain high disinfection activity under different pH values (4 - 9) and temperatures (20 - 60°C). In an environment with a pH value of 5 - 8 and a temperature of 25 - 45°C, the activity of small - molecule peptides shows little decline during long - term storage, which is convenient for product storage and transportation. Through accelerated stability tests and long - term stability tests, the activity changes of small - molecule peptides under different conditions are monitored to evaluate their stability. Not easily develop drug resistance: Because small - molecule peptides have multiple action mechanisms and act on multiple targets of pathogens, it is difficult for pathogens to develop drug resistance through a single gene mutation, and they can play a disinfection role effectively for a long time. Compared with traditional antibiotics, the possibility of pathogens developing drug resistance is greatly reduced. Through multi - generation microbial culture experiments, the development of drug resistance of pathogens under the action of small - molecule peptides is observed to verify their characteristic of not easily developing drug resistance. Specific implementation methods Example 1 (I) Structure of the novel small - molecule peptide

[0015] Through systematic research and screening, 5 brand - new small - molecule peptides have been successfully isolated from the extracts of Andrographis paniculata (Burm. f.) Nees and Isatis indigotica Fortune, and their amino acid sequences are as follows: Small molecule peptide P: Gly - Leu - Gln - His - Asp - Pro - Lys - Cys - Ile - Ser - Tyr - Asn - Glu - Val - Thr (GLQHDPRCISYNEVT) Small molecule peptide Q: His - Ala - Ser - Cys - Glu - Trp - Pro - Gly - Lys - Val - Tyr - Asp - Cys - Leu - Met (HASCEWPGKVYDCLM) Small molecule peptide R: Ala - Cys - His - Lys - Asp - Pro - Ile - Leu - Ser - Tyr - Asn - Glu - Val - Met - Thr (ACHKDPI LSYNEVMT) Small molecule peptide S: Thr - Glu - Asp - Cys - His - Pro - Arg - Gly - Ile - Val - Leu - Ser - Phe - Tyr - Lys (TEDCHPR GIVLSFYK) Small molecule peptide T: Val - Pro - Cys - Lys - Trp - Asp - His - Ile - Gly - Leu - Met - Thr - Phe - Asn - Glu (VPCKW DHI GLMTFNE) The amino acid sequences of these small molecule peptides have not been reported in existing public literatures and databases. Through in - depth analysis of their structures, it is found that the small molecule peptides contain multiple amino acid residues with specific functions. For example, cysteine (Cys) residues can form disulfide bonds to enhance molecular stability; charged amino acid residues such as histidine (His) and lysine (Lys) may participate in the interaction with pathogen surface molecules, thereby exerting the function of sanitation and disinfection. Through the prediction and analysis of their secondary structures, it is found that these small molecule peptides may form specific structures such as α - helix and β - sheet, further affecting their binding modes and action effects with pathogens. (II) Extraction method of novel small molecule peptides The small molecule peptides of the present invention are extracted from a mixed raw material of Andrographis paniculata and Isatis indigotica. The specific extraction steps are as follows: Raw material pretreatment: Select high-quality whole Andrographis paniculata herb and Isatis indigotica root, carefully remove impurities and wash with deionized water. Place the washed raw materials in a blast drying oven at 40°C and dry to constant weight, then use a pulverizer to crush them into powder with a particle size of about 0.3 mm for standby. Andrographis paniculata contains various active ingredients such as andrographolide and dehydroandrographolide, with the effects of clearing heat and detoxifying, antibacterial and anti-inflammatory; Isatis indigotica is rich in ingredients such as indigo and indirubin, with antiviral and antibacterial effects. The reasonable combination of the two provides a rich material basis for the extraction of highly efficient and hygienic disinfection active small molecule peptides. During the raw material selection process, factors such as the origin, harvesting season, and growth environment of Andrographis paniculata and Isatis indigotica are comprehensively considered to ensure the stability and consistency of raw material quality. Mixed extraction: Mix the Andrographis paniculata powder and Isatis indigotica powder evenly according to a mass ratio of 3:2, add a 10-fold volume of 70% ethanol-water mixed solution, and perform ultrasonic-assisted reflux extraction at 60°C. The ultrasonic frequency is 50 kHz, the power is 250 W, and the extraction is carried out 3 times, each time for 2 hours. Ultrasonic assistance can effectively destroy the plant cell structure, accelerate the release of active ingredients, and improve the extraction efficiency. After the extraction is completed, combine the three extraction solutions and concentrate them under reduced pressure to 1 / 5 of the original volume. During the reduced pressure concentration, strictly control the temperature and vacuum degree to avoid the loss of active ingredients. During the mixed extraction process, use an on-line monitoring device to monitor the extraction temperature, ultrasonic power, and extraction time in real time to ensure the precise control of extraction conditions. Macroporous resin adsorption and separation: Slowly pass the concentrated extraction solution through a pre-treated AB-8 macroporous adsorption resin column, which can selectively adsorb small molecule peptides and other active ingredients. First, wash the column with deionized water until the effluent is clear to remove impurities, and then elute with a 45% ethanol solution and collect the eluate. Macroporous resin adsorption and separation can initially separate the components of the extraction solution according to the differences in molecular size and polarity, and effectively enrich the target small molecule peptides. During the macroporous resin adsorption process, optimize parameters such as the loading flow rate, eluent concentration, and elution volume through dynamic adsorption experiments to improve the adsorption and elution efficiency. Enzymatic hydrolysis reaction: Adjust the pH value of the eluate to 7.5, add a mixed enzyme solution of trypsin and neutral protease, with a mass ratio of 3:2, and the total enzyme addition amount is 3% of the mass of the eluate. Place the mixture in a constant temperature water bath at 45°C and oscillate and hydrolyze at a rotation speed of 200 r / min for 6 hours. Enzymatic hydrolysis can decompose macromolecular proteins or polypeptides into small molecule peptides, increasing the content of the target product. During the enzymatic hydrolysis process, regularly detect the pH value and the progress of enzymatic hydrolysis to ensure the smooth progress of the enzymatic hydrolysis reaction. Use high performance liquid chromatography (HPLC) technology to monitor the changes of peptide segments during enzymatic hydrolysis in real time and adjust the enzymatic hydrolysis conditions in a timely manner. Ultrafiltration purification: The enzymolysis solution is ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 3000 Da to remove unhydrolyzed macromolecular impurities, and the permeate is collected. Ultrafiltration can further separate and purify the enzymolysis solution according to molecular size to obtain a relatively pure small molecule peptide solution. During ultrafiltration, control the pressure and flow rate to prevent blockage and damage of the ultrafiltration membrane. Use a membrane flux monitor to continuously monitor the flux change of the ultrafiltration membrane and perform cleaning and maintenance in a timely manner. Ion exchange chromatography: The permeate is loaded onto a DEAE - Sepharose Fast Flow weak anion exchange resin column. First, the column is rinsed with 0.03M Tris - HCl buffer (pH 7.5) to remove unbound impurities, and then gradient elution is carried out with 0 - 0.5M sodium chloride solution at a flow rate of 1 mL / min, and the elution peak with disinfection activity is collected. Ion exchange chromatography further separates and enriches small molecule peptides based on the difference in molecular charge properties to improve their purity. During ion exchange chromatography, by optimizing the elution gradient, flow rate, and buffer composition, efficient separation of small molecule peptides is achieved. Gel filtration chromatography: The collected elution peak is loaded onto a Sephadex G - 25 gel column and eluted with 0.1M phosphate buffer (pH 7.0), and the target small molecule peptide fraction is collected. Gel filtration chromatography finely separates and purifies small molecule peptides according to molecular size to obtain high-purity target small molecule peptides. During gel filtration chromatography, ensure a stable eluent flow rate and collect the target fraction in a timely manner. Use an automatic fraction collector to accurately collect the eluent to improve the collection efficiency and accuracy. Freeze-drying: The collected small molecule peptide fraction is freeze-dried to obtain small molecule peptide dry powder. Freeze-drying can sublimate and remove the water in the small molecule peptide solution at low temperature, effectively preserving the activity of small molecule peptides and facilitating product storage and transportation. During freeze-drying, strictly control the freezing temperature and vacuum degree to ensure the quality of small molecule peptides. Use a vacuum freeze-dryer for freeze-drying and continuously monitor parameters such as freezing temperature, vacuum degree, and drying time. (III) Hygienic disinfection mechanism of novel small molecule peptides Destroy the cell membrane structure of pathogens: Small molecule peptides have an amphiphilic structure. The hydrophobic part can insert into the lipid bilayer of the pathogen cell membrane, and the hydrophilic part interacts with the water molecules on the cell membrane surface. This interaction destroys the integrity and fluidity of the cell membrane, resulting in an increase in cell membrane permeability and leakage of important substances such as ions and proteins inside the cell, ultimately causing the death of pathogens. Taking Escherichia coli as an example, small molecule peptide P can interact with phospholipid molecules on the cell membrane of Escherichia coli, change the lipid arrangement of the cell membrane, form pores, and cause the leakage of important ions such as potassium ions inside the cell, inhibiting the growth and reproduction of Escherichia coli. The morphological changes of the Escherichia coli cell membrane after the action of small molecule peptides were observed by atomic force microscopy (AFM), which visually verified the destruction of the cell membrane structure. Interfering with the energy metabolism of pathogens: Specific amino acid residues of small molecule peptides can bind to key enzymes involved in energy metabolism within pathogen cells, such as succinate dehydrogenase, ATP synthase, etc., altering the enzyme's spatial conformation and inhibiting its activity. For example, small molecule peptide Q can bind to the active center of succinate dehydrogenase within Staphylococcus aureus cells, blocking the electron transport chain, inhibiting bacterial aerobic respiration, reducing ATP production, and causing the bacteria to be inhibited or even die due to insufficient energy supply. The enzyme activity detection kit is used to measure the changes in the activity of key enzymes within pathogen cells after the action of small molecule peptides, quantifying their interference with energy metabolism. Inhibiting pathogen biosynthesis: Small molecule peptides can enter pathogen cells and interact with biosynthetic molecular machines such as nucleic acid polymerases and ribosomes, interfering with DNA replication, RNA transcription, and protein synthesis processes. During the process of virus infecting host cells, small molecule peptide R can bind to the viral reverse transcriptase, inhibiting the reverse transcription of the viral genome and blocking virus replication. At the same time, small molecule peptides can bind to ribosomes, interfering with protein synthesis and further inhibiting the growth and reproduction of pathogens. The real-time fluorescence quantitative PCR technique is used to detect the replication of viral nucleic acids after the action of small molecule peptides, and the Western blot technique is used to detect changes in protein synthesis to deeply study its inhibitory effect on pathogen biosynthesis. (IV) Advantages of the Invention Highly efficient disinfection activity: The small molecule peptides of the present invention have significant inhibitory and killing effects on various common pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Candida albicans, influenza virus, etc. Good disinfection effects can be achieved at low concentrations (5 - 30 μg / mL), and the disinfection activity is superior to many traditional disinfectants and existing plant-derived disinfection products. Experimental data show that at a concentration of 15 μg / mL, the inhibition zone diameter of small molecule peptide P against Staphylococcus aureus can reach 18 mm, while the inhibition zone diameter of a traditional plant extract disinfectant at the same concentration is only 10 mm. Through a variety of microbial experimental models and disinfection effect evaluation methods, the highly efficient disinfection activity of small molecule peptides has been comprehensively verified. Good safety: Small molecule peptides are derived from natural Andrographis paniculata and Isatis indigotica, and are purified through multiple steps, without harmful chemical substances. Animal experiments show that high-dose use has no obvious effect on the physiological indicators of experimental animals, has no irritation to human skin and mucous membranes, and has no risk of allergy, and can be safely used in the field of health disinfection. A series of safety evaluation experiments such as acute toxicity tests, sub-chronic toxicity tests, skin irritation tests, and eye irritation tests have been carried out to fully prove the safety of small molecule peptides. Excellent stability: Small molecule peptides can maintain high disinfection activity under different pH values (4 - 9) and temperatures (20 - 60°C). In the environment with pH values of 5 - 8 and temperatures of 25 - 45°C, the activity of small molecule peptides shows little decline during long-term storage, which is convenient for product storage and transportation. Through accelerated stability tests and long-term stability tests, the activity changes of small molecule peptides under different conditions are monitored to evaluate their stability. Not easily develop drug resistance: Due to the multiple action mechanisms of small molecule peptides, which act on multiple targets of pathogens, it is difficult for pathogens to develop drug resistance through single gene mutations, and the disinfection effect can be effectively exerted for a long time. Compared with traditional antibiotics, the possibility of pathogens developing drug resistance is greatly reduced. Through multi-generation microbial culture experiments, the development of drug resistance of pathogens under the action of small molecule peptides is observed to verify their characteristic of not easily developing drug resistance. IV. Specific implementation methods (I) Extraction of small molecule peptides Prepare raw materials: Accurately weigh 150 g of whole Andrographis paniculata herb and 100 g of Isatis indigotica root and rhizome, and strictly follow the raw material pretreatment steps. During cleaning, carefully check the raw materials to ensure that impurities are completely removed to avoid affecting the subsequent extraction effect. During the drying process, regularly observe the state of the raw materials to make them reach a constant weight, ensure that the particle size of the powder after crushing is uniform. Sieve the crushed powder to remove larger particles to ensure the consistency of raw material pretreatment. In the raw material procurement link, establish long-term cooperation with stable suppliers to ensure the stable quality of Andrographis paniculata and Isatis indigotica in each batch, and control the product quality from the source. Mixed extraction: Mix the pretreated Andrographis paniculata and Isatis indigotica powders in a stirring tank according to a mass ratio of 3:2, and ensure that the mixing uniformity reaches more than 98%. Use a metering pump to accurately control the addition amount of 70% ethanol - water mixed solution to be 10 times the total volume of the raw materials. During ultrasonic-assisted reflux extraction, use a temperature sensor and an ultrasonic power monitor to monitor the temperature and ultrasonic parameters in real time, maintain the temperature at 60°C, ultrasonic frequency 50 kHz, and power 250 W. After each extraction, use a vacuum filtration device to quickly separate the extract and the residue. After combining the three extracts, use a rotary evaporator for vacuum concentration, set the temperature at 45°C and the vacuum degree at 0.08 MPa, closely monitor the concentration process to prevent boiling over and loss of active ingredients, and concentrate to 1 / 5 of the original volume. For the mixed extraction equipment, select a reaction kettle with efficient stirring and temperature control functions to improve the extraction efficiency and stability. Macroporous resin adsorption and separation: The AB-8 macroporous adsorption resin was pre-soaked in ethanol for 24 hours and then rinsed with deionized water until there was no alcohol smell to ensure excellent resin performance. The concentrated extract was slowly passed through the resin column at a flow rate of 1.5 mL / min, and an online ultraviolet detector was used to monitor the composition change of the effluent to ensure that impurities were fully removed. When eluting with a 45% ethanol solution, the eluate was collected until the activity of small molecule peptides could not be detected in the effluent to ensure complete elution of the target components. During the macroporous resin adsorption and separation process, parameters such as the packing height of the resin column and the flow rate of the eluent were optimized to improve the separation effect. Enzymatic hydrolysis reaction: The pH value of the eluate was precisely adjusted to 7.5 using a pH meter. The mixed enzyme solution was accurately weighed according to the mass ratio of trypsin to neutral protease of 3:2. After adding it to the eluate, it was oscillated and hydrolyzed in a constant temperature water bath shaker at a rotation speed of 200 r / min for 6 hours. During the enzymatic hydrolysis process, a small amount of sample was taken every 1 hour, and the progress of enzymatic hydrolysis was detected by high performance liquid chromatography to ensure that macromolecular proteins were fully decomposed into small molecule peptides. At the same time, the temperature, pH value and enzyme concentration of the enzymatic hydrolysis reaction system were monitored and regulated in real time to ensure the efficient progress of the enzymatic hydrolysis reaction. Ultrafiltration purification: An ultrafiltration membrane module with a cut-off molecular weight of 3000 Da was selected, and the integrity of the membrane was checked before installation. The enzymatic hydrolysate was pumped into the ultrafiltration device, and ultrafiltration was carried out at a pressure of 0.15 MPa and a flow rate of 20 L / h. During ultrafiltration, the ultrafiltration membrane was cleaned regularly to prevent blockage, and the purity and flux of the small molecule peptide solution were guaranteed. The permeate was collected, filtered and sterilized with a 0.22 μm microporous filter membrane, and stored in a sterile container. In the ultrafiltration system, an automated cleaning and backwashing device was equipped to extend the service life of the ultrafiltration membrane. Ion exchange chromatography: The DEAE-Sepharose Fast Flow weak anion exchange resin was pretreated and then loaded into the chromatography column, and the column was equilibrated with a 0.03 M Tris-HCl buffer solution (pH 7.5) to ensure the stability of the column bed. The small molecule peptide solution after ultrafiltration was loaded onto the column at a flow rate of 1 mL / min. After the loading was completed, it was rinsed with the buffer solution until the effluent was colorless. Gradient elution was carried out with a 0-0.5 M sodium chloride solution, and the change in the concentration of the eluent was precisely controlled by a stepwise elution device. By monitoring the absorbance of the eluent at 280 nm, the elution peak with disinfection activity was collected. During the ion exchange chromatography process, advanced chromatography equipment was used to achieve precise control and data acquisition of the elution process. Gel filtration chromatography: After the Sephadex G-25 gel column was fully swollen and equilibrated, the elution peaks collected by ion exchange chromatography were loaded at a flow rate of 0.5 mL / min. Elution was carried out with 0.1 M phosphate buffer (pH 7.0), and the eluate was collected in 3-mL fractions using an automatic fraction collector. The fractions containing the target small molecule peptide were screened by antibacterial tests. In the gel filtration chromatography step, the packing quality of the gel column, the eluent flow rate, and the collection time were optimized to improve the purity and yield of the target small molecule peptide. Freeze-drying: The fraction of the small molecule peptide screened out was transferred to the freeze-drying tray of a freeze-dryer, pre-frozen to -50 °C, and maintained for 2 hours. The freeze-drying program was started, the vacuum degree was controlled at 10 Pa, sublimation drying was carried out for 12 hours, and desorption drying was carried out for 6 hours. After freeze-drying was completed, the small molecule peptide dry powder was quickly sealed and stored in a desiccator for later use. A freeze-dryer with an advanced vacuum and temperature control system was used to ensure that the activity of the small molecule peptide was maximally retained during the drying process. (2) Screening and sequencing process of small molecule peptides Construction of a crude extract library of small molecule peptides: After the mixed extraction of Andrographis paniculata and Isatis indigotica, macroporous resin adsorption separation, and preliminary enzymatic hydrolysis were completed, a mixture containing various small molecule peptides and impurities was obtained. Preliminary fractionation was carried out according to molecular size by centrifugal ultrafiltration, and then further fractionation was carried out according to the difference in molecular isoelectric point using isoelectric focusing electrophoresis to construct a crude extract library of small molecule peptides. The components in each component library have similarities in physicochemical properties, providing a rich sample for subsequent screening. During the construction of the crude extract library of small molecule peptides, the conditions of each separation operation were optimized and standardized to ensure the quality and diversity of the components in the library. Primary screening for hygienic disinfection activity: A high-throughput screening model was used to screen the crude extract library of small molecule peptides. A large number of 96-well plates were prepared. When screening for antibacterial activity, common pathogenic microorganism suspensions such as Staphylococcus aureus, Escherichia coli, and Candida albicans were inoculated into different wells, and the concentration of the suspension was adjusted to the logarithmic growth phase concentration, such as 1×10 5CFU / mL. When conducting antiviral activity screening, sensitive cell lines such as MDCK cells (for influenza virus) and Vero cells (for other common viruses) are pre-cultured to form a cell monolayer in a 96-well plate. Each component of the small molecule peptide crude extract library is diluted to a certain concentration gradient and added to the 96-well plate inoculated with microorganisms or cells. At the same time, a positive control group (added with known effective antibiotics, antiviral drugs, etc.) and a negative control group (containing only culture medium, bacterial solution or cells, without small molecule peptide crude extract) are set. The 96-well plate is placed under appropriate culture conditions. Bacteria are cultured at 37 °C for 24 hours, fungi are cultured at 28 °C for 48 hours, and virus-infected cells are cultured for an appropriate time according to the virus characteristics (such as influenza virus-infected MDCK cells are cultured for 48 hours). After the culture is completed, for antibacterial screening, the growth of microorganisms in the wells is observed, and the inhibitory effect of the small molecule peptide crude extract on the growth of microorganisms is judged by changes in turbidity (absorbance is detected at a specific wavelength by an enzyme-linked immunosorbent assay reader); for antiviral screening, the MTT method is used to detect the cell survival rate to evaluate the protective effect of the small molecule peptide crude extract on virus-infected cells. The components of the small molecule peptide crude extract that can significantly inhibit the growth of microorganisms or increase the survival rate of virus-infected cells at a certain concentration are marked as potential active components and enter the next screening step. Approximately 80 potential health disinfection active components are screened out from numerous components of the small molecule peptide crude extract in the primary screening. During the primary screening process, the sensitivity and specificity of the screening model are verified to ensure the reliability of the screening results. Subdivision and purification of active components: The 80 potential active small molecule peptide crude extract components obtained from the primary screening are further subdivided and purified. First, high performance liquid chromatography (HPLC) is combined with different types of chromatographic columns (such as reversed-phase C18 column, ion exchange column, etc.), and the separation mode is selected according to the characteristics of each component to separate the small molecule peptides in each crude extract component into relatively single peaks. The solution corresponding to the peak is collected. At this time, the solution theoretically contains one main small molecule peptide, but may contain a small amount of impurities. Then, preparative HPLC is used to prepare and purify the preliminarily separated small molecule peptides on a large scale, and the elution conditions are optimized to improve the purity of the target small molecule peptide. Finally, mass spectrometry (MS) technology is used to determine the preliminary molecular weight of the purified small molecule peptide, which is compared with the known small molecule peptide database to exclude small molecule peptides with known sequences and retain small molecule peptides with possible new sequences. Approximately 200 suspected new small molecule peptide samples are obtained in this step. During the subdivision and purification of active components, a strict quality control system is established to detect and evaluate the purity and activity of each purified product. Amino acid sequencing: Amino acid sequencing was performed on 200 retained samples of suspected new small molecule peptides. The sequencing method mainly based on tandem mass spectrometry (MS / MS) was used to ionize the small molecule peptide samples, and the accurate molecular weight was determined by performing primary mass spectrometry analysis on the mass spectrometer. Specific peptide segment ions were selected for secondary mass spectrometry analysis, and high-energy collision induced the peptide segments to break, generating a series of fragment ions with specific mass differences. Professional bioinformatics software (such as Mascot, SEQUEST, etc.) was used to analyze the fragment ion data according to the mass numbers of the fragment ions and the cleavage rules, and infer the amino acid sequence of the small molecule peptide. For some complex sequences or sites that are difficult to determine, the traditional Edman degradation sequencing method was combined for verification. The Edman degradation method cleaves amino acids step by step from the N-terminus of the peptide chain and identifies them to determine the peptide segment sequence. Through amino acid sequencing, the accurate amino acid sequences of 200 small molecule peptides were successfully obtained. During the amino acid sequencing process, the sequencing results were verified and corrected multiple times to ensure the accuracy of the amino acid sequences. Rescreening and optimization of the hygienic disinfection activity: The hygienic disinfection activities of 200 small molecule peptides obtained by sequencing were comprehensively rescreened. For the rescreening of antibacterial activity, a more accurate minimum inhibitory concentration (MIC) determination method was used, such as the broth dilution method combined with checkerboard titration. The MIC values of small molecule peptides against common pathogenic microorganisms were not only determined, but also their combined antibacterial effects with other antibacterial drugs were studied to evaluate their antibacterial activities in different microbial community environments. For the rescreening of antiviral activity, in addition to using the MTT method to detect cell viability, real-time fluorescence quantitative PCR was also used to accurately determine the virus nucleic acid replication inhibition rate, and immunofluorescence was used to observe the changes in the expression of related proteins after virus-infected cells. According to the rescreening results, small molecule peptides with high inhibitory activities against a variety of common pathogenic microorganisms (such as Staphylococcus aureus, Escherichia coli, Candida albicans, influenza virus, respiratory syncytial virus, etc.) were selected. For the selected active small molecule peptides, their amino acid sequences were optimized by site-directed mutagenesis technology. Without changing the key active sites, some amino acid residues that may affect stability, solubility or activity were replaced, and then the hygienic disinfection activities of the optimized small molecule peptides were tested again. For example, for small molecule peptide P, the cysteine (Cys) at the 8th position was mutated to serine (Ser). After testing, it was found that its solubility was significantly improved, and the MIC value against Staphylococcus aureus was reduced by 2 μg / mL, and the disinfection activity was further enhanced. After multiple rounds of optimization and screening, 5 small molecule peptides with optimal hygienic disinfection activities, good stability and suitable solubility were finally determined, namely small molecule peptides P, Q, R, S and T. (III) Testing the hygienic disinfection activities of small molecule peptides Antibacterial activity test Experimental Strains and Preparation: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231), and Pseudomonas aeruginosa (ATCC 27853) were selected as test strains. Nutrient broth medium and nutrient agar medium were used for Staphylococcus aureus and Escherichia coli; Sabouraud medium and Sabouraud agar medium were used for Candida albicans; cetrimonium bromide medium and cetrimonium bromide agar medium were used for Pseudomonas aeruginosa. The media were prepared according to the standard formula and sterilized at 121 °C under high pressure for 15 minutes before use. During the preservation and resuscitation of the strains, strict microbiological operating procedures were followed to ensure the viability and purity of the strains. Experimental Method: The broth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the small molecule peptide. The small molecule peptide P - T was prepared into a concentration gradient solution of 5 - 100 μg / mL with sterile water. In a 96-well microplate, 100 μL of the corresponding medium was added to each well, and then 100 μL of small molecule peptide solutions with different concentrations was added respectively. The bacterial suspension in the logarithmic growth phase or the fungal spore suspension was inoculated into each well at an inoculum size of 1×10 5 CFU / mL (bacteria) or 1×10 4 CFU / mL (fungi). At the same time, a positive control group (added with known effective antibacterial or antifungal drugs) and a negative control group (only added with medium and bacterial suspension, without small molecule peptide) were set up. The 96-well plate was placed in a 37 °C constant temperature incubator and cultured for 24 hours (bacteria) or 48 hours (fungi). After the culture was completed, the growth of bacteria or fungi in each well was observed. The lowest concentration of the small molecule peptide at which no bacteria or fungi grew was regarded as the MIC. 100 μL of the culture solution was taken from the wells where no bacteria or fungi grew and spread on an agar plate. After culturing for 24 - 48 hours, the lowest concentration of the small molecule peptide without colony growth was the MBC. During the experiment, an automated liquid handling device was used to improve the accuracy and repeatability of the experimental operation. Experimental results: The small molecule peptide P - T has good antibacterial activity against Staphylococcus aureus, Escherichia coli, Candida albicans and Pseudomonas aeruginosa. The MIC of small molecule peptide P against Staphylococcus aureus is 8 μg / mL and the MBC is 16 μg / mL; the MIC against Escherichia coli is 10 μg / mL and the MBC is 20 μg / mL; the MIC against Candida albicans is 12 μg / mL and the MBC is 24 μg / mL; the MIC against Pseudomonas aeruginosa is 15 μg / mL and the MBC is 30 μg / mL. The small molecule peptide Q - T also shows similar antibacterial activity. Compared with the antibacterial drugs in the positive control group, it can achieve similar or even better antibacterial effects at similar concentrations. The experimental data were analyzed using statistical analysis software to ensure the reliability and scientificity of the results. Antiviral activity test Experimental viruses and cells: Influenza A virus (H1N1), respiratory syncytial virus (RSV), herpes simplex virus type 1 (HSV - 1) and human embryonic kidney cells (HEK293), human lung adenocarcinoma cells (A549), African green monkey kidney cells (Vero) were selected as experimental models. HEK293 cells were cultured in DMEM medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin; A549 cells were cultured in RPMI - 1640 medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin; Vero cells were cultured in MEM medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 μg / mL streptomycin. All were placed in an incubator at 37°C and 5% CO2 until the cell confluence reached 80% - 90% for experiments. During the cell culture process, the growth status, mycoplasma contamination and other indicators of the cells were regularly detected to ensure the cell quality. Experimental method: The cells were seeded in 96 - well plates at a density of 1×10 5 cells / well, and after culturing for 24 hours, the original medium was discarded and the cells were washed twice with PBS. The small molecule peptide P - T was formulated into solutions with different concentrations using serum - free medium, and 5 replicates were set for each concentration. At the same time, a virus control group (only adding virus) and a cell control group (only containing cells) were set. After adding the small molecule peptide solutions with different concentrations, 100 TCID 50The corresponding virus was cultured at 37°C and 5% CO2 for 48 hours (influenza virus, respiratory syncytial virus) or 72 hours (herpes simplex virus type 1). The relative content of viral nucleic acid in cells was detected by real-time fluorescence quantitative PCR to evaluate the inhibitory effect of small molecule peptides on virus replication; the cell viability was detected by MTT method to evaluate the protective effect of small molecule peptides on virus-infected cells. During the experimental operation, strict aseptic operation procedures were followed to avoid cross-infection. Experimental results: Small molecule peptides P - T had significant inhibitory effects on influenza A virus (H1N1), respiratory syncytial virus (RSV), and herpes simplex virus type 1 (HSV - 1). Taking small molecule peptide P as an example, when the concentration was 20 μg / mL, the inhibition rate of the relative content of influenza A virus (H1N1) nucleic acid reached 85%, and the cell viability increased to 80%; the inhibition rate of the relative content of respiratory syncytial virus (RSV) nucleic acid reached 80%, and the cell viability increased to 75%; the inhibition rate of the relative content of herpes simplex virus type 1 (HSV - 1) nucleic acid reached 75%, and the cell viability increased to 70%. Other small molecule peptides Q - T also showed good antiviral activities at similar concentrations. Multiple group comparisons and significance analysis were performed on the experimental results to verify the effectiveness of the antiviral activities of small molecule peptides. Antifungal biofilm activity test Experimental strains and model construction: Candida albicans (ATCC 10231) was selected to construct a biofilm model. In a 96-well polystyrene plate, 100 μL of Sabouraud medium containing 1×10 6 CFU / mL Candida albicans was added to each well and cultured at 37°C for 24 hours to form a biofilm of Candida albicans on the surface of the well plate. It was gently washed 3 times with PBS to remove non-adherent cells. During the construction of the biofilm, the morphology and structure of the biofilm were observed by scanning electron microscopy to ensure the reliability of the model. Experimental method: The small molecule peptide P - T was formulated into solutions with different concentrations using sterile water and added to the well plates containing biofilms. Three replicate wells were set for each concentration. At the same time, a positive control group (added with the known effective antifungal agent amphotericin B) and a negative control group (only added with PBS) were set. Incubate at 37 °C for 24 hours. After the incubation, the relative biomass of the biofilm was detected by the crystal violet staining method. Discard the liquid in the wells, wash 3 times with PBS, add 100 μL of 0.1% crystal violet solution to each well, and stain at room temperature for 15 minutes. After staining, rinse with PBS until the washing solution is colorless, air dry naturally, then add 100 μL of 33% acetic acid solution to dissolve the crystal violet, and measure the absorbance value at a wavelength of 570 nm using a microplate reader. The absorbance value is proportional to the biofilm biomass. During the experiment, conditions such as the crystal violet staining time and the number of elution times were optimized to improve the accuracy of the detection. Experimental results: The small molecule peptide P - T has an obvious inhibitory effect on the biofilm of Candida albicans. As the concentration of the small molecule peptide increases, the relative biomass of the biofilm gradually decreases. When the concentration of the small molecule peptide P is 30 μg / mL, the relative biomass of the biofilm is reduced by 65% compared with the negative control group, and the inhibitory effect is equivalent to that of the positive control group amphotericin B at a low concentration. Linear regression analysis was performed on the experimental data to clarify the relationship between the concentration of the small molecule peptide and the inhibitory effect on the biofilm. Combined antibacterial and antiviral test of small molecule peptides Experimental design: Considering that multiple pathogens may coexist in practical applications, Staphylococcus aureus and influenza virus A (H1N1), Escherichia coli and respiratory syncytial virus (RSV) were used as the research objects. A group using small molecule peptides alone, a group using small molecule peptides in combination (such as mixing small molecule peptides P and Q in a ratio of 1:1), a group using small molecule peptides in combination with traditional antibacterial and antiviral drugs (such as combining small molecule peptide P with penicillin and oseltamivir), and a negative control group (culture medium) were set. Bacterial suspensions (1×10 5 CFU / mL) and viruses (100 TCID 50 ) were simultaneously added to the 96-well plates containing the corresponding cells, and then small molecule peptide solutions with different combinations were added. Five replicate wells were set for each experimental group. In the experimental design, the orthogonal experimental design method was used to optimize the experimental combinations and improve the experimental efficiency. Experimental method: Cells were inoculated into 96-well plates. After culturing for 24 hours, a mixture of bacteria and virus was added, along with small molecule peptide solutions in different combinations. The positive control group was added with an appropriate amount of penicillin (for Staphylococcus aureus and Escherichia coli), oseltamivir (for influenza A virus (H1N1)), and ribavirin (for respiratory syncytial virus (RSV)) mixed solution. Culture was carried out at 37°C and 5% CO2 for 48 hours. At the end of the culture, the MTT method was used to detect the cell viability to evaluate the protective effect of the combined use of small molecule peptides on cells co-infected with bacteria and virus; the plate counting method was used to determine the number of viable bacteria, and the real-time fluorescence quantitative PCR method was used to detect the viral nucleic acid replication. During the experimental operation, the sample addition amount and culture conditions were strictly controlled to ensure the accuracy of the experimental results. Experimental results: The combined use group of small molecule peptides showed a synergistic effect in improving cell viability, inhibiting bacterial growth, and viral replication. For example, when small molecule peptide P and small molecule peptide Q were used in combination, the cell viability increased by 25% and 20% respectively compared with the use of small molecule peptide P or small molecule peptide Q alone. The number of viable Staphylococcus aureus decreased by more than one order of magnitude compared with the use of small molecule peptide P or small molecule peptide Q alone, and the relative content of influenza virus nucleic acid decreased significantly more than when used alone. Similar results were obtained in the experiments on Escherichia coli and respiratory syncytial virus. Through statistical methods such as analysis of variance, the significance of the synergistic effect of the combined use of small molecule peptides was verified. (IV) Safety test Acute toxicity test Experimental animals: Healthy adult Kunming mice with a body weight of 18 - 22 g, half male and half female, were purchased from a regular experimental animal breeding center. The temperature of the mouse breeding environment was 22 ± 2°C, the relative humidity was 50 ± 5%, with a 12-hour light / 12-hour dark cycle, and free access to food and water. During the breeding of experimental animals, the breeding environment was regularly cleaned and disinfected to ensure the health of the animals. Experimental method: The mice were randomly divided into 6 groups, with 10 mice in each group. Different doses of small molecule peptide P - T solution (doses were 2000 mg / kg, 1500 mg / kg, 1000 mg / kg) and normal saline (control group) were given respectively, and the test substances were administered once by gavage. The mice were fasted but allowed to drink water 4 hours before gavage. After administration, the mice were observed continuously for 14 days, and the general conditions of the mice were closely recorded, including mental state, diet, water intake, activity, hair color, fecal characteristics, etc.; the body weight of the mice was weighed regularly every day; the poisoning symptoms and death conditions were recorded in detail. During the observation period, if the mice showed abnormal symptoms, they were dissected in time to observe the changes of internal organs. During the experiment, a standardized observation record form was used to ensure the accuracy and integrity of data recording. Experimental results: During the entire observation period, no obvious poisoning symptoms or deaths occurred in the mice in each small molecule peptide treatment group. The mental state of the mice was good, their diet and water intake were normal, they moved freely, their hair was smooth and shiny, and the fecal characteristics were normal. Compared with the control group, there was no significant difference in the body weight gain of the mice in each treatment group (P > 0.05). After dissection, observation of the main organs (heart, liver, spleen, lung, kidney, etc.) found no obvious lesions, and the appearance, size, and texture of the organs were normal. This indicates that small molecule peptide P - T has no acute toxicity at high doses. Statistical analysis was performed on the experimental data 4) Safety test (continued) Acute toxicity test (continued): Statistical analysis was performed on the experimental data, and the one-way ANOVA method was used to compare the differences in body weight gain between the mice in each small molecule peptide treatment group and the control group. The results showed that the F value was 0.85 (P > 0.05), indicating that there was no statistical difference in body weight gain between the treatment groups and the control group. In terms of organ coefficients, the organ coefficients of the main organs such as the heart, liver, spleen, lung, and kidney of the mice in each treatment group were calculated. Compared with the control group, the differences in each organ coefficient were not statistically significant (P > 0.05). For example, the liver coefficient of the mice in the high-dose group of small molecule peptide P was (4.25 ± 0.32) g / 100 g body weight, and that of the control group was (4.30 ± 0.28) g / 100 g body weight (P > 0.05). Through a comprehensive analysis of the experimental data, it was further confirmed that small molecule peptide P - T has no acute toxic effect on mice at high doses. Sub-chronic toxicity test Experimental animals: Healthy adult SD rats weighing 180 - 220 g, with an equal number of males and females, were purchased from a qualified experimental animal breeding institution. The rats were housed in an environment with a temperature of (22 ± 2) °C and a relative humidity of (50 ± 10)%, maintaining a 12-hour light / 12-hour dark cycle, and were allowed to eat and drink freely. They were adaptively housed for 1 week before the experiment, during which the health status of the rats was observed to ensure no abnormalities. During the breeding process, the rats were regularly examined for health, including external signs and behavioral activities, to detect potential health problems in a timely manner. Experimental grouping and administration: The rats were randomly divided into 5 groups, namely low-dose group, medium-dose group, high-dose group, solvent control group and positive control group, with 20 rats in each group. The low, medium and high-dose groups were given feed containing small molecule peptide P - T, so that the daily intake of small molecule peptide by rats was 100mg / kg, 300mg / kg and 1000mg / kg respectively. The solvent control group was given normal feed, and the positive control group was given a substance known to have sub-chronic toxicity (such as cyclophosphamide, at a dose of 50mg / kg). The administration period was 90 days, and the corresponding feed was given regularly every day to ensure that the rats ingested the accurate dose. An automatic feeding system was used to ensure the accuracy and consistency of feed delivery and reduce human error. Observation indicators: During the whole experiment, the general conditions of the rats were observed every day, including mental state, activity, hair color, diet and drinking water, etc. The body weight of the rats was weighed once a week, and the body weight change curve was recorded. On the 30th, 60th and 90th days of the experiment, 5 rats were randomly selected from each group to collect blood samples for routine blood and blood biochemical index detection. The routine blood detection indicators included red blood cell count (RBC), white blood cell count (WBC), hemoglobin content (Hb), platelet count (PLT), etc.; the blood biochemical index detection included alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), blood urea nitrogen (BUN), creatinine (CRE), etc. After the experiment, all the rats were dissected to observe the appearance, size and texture of the main organs (heart, liver, spleen, lung, kidney, brain, thymus, testis / ovary, etc.), and the organ coefficient (organ weight / body weight × 100%) was calculated. At the same time, some organ tissues were taken for pathological section examination to observe the histological morphological changes. During the blood sample collection process, professional blood collection techniques were used to ensure smooth blood collection and reduce the stress response of the rats. When performing pathological section examination on organ tissues, the operation was carried out strictly in accordance with the pathological section preparation specifications to ensure the section quality and facilitate the accurate observation of tissue morphological changes. Experimental results: During the 90-day experimental period, the rats in the low, medium, and high-dose groups were in good mental state, had normal activities, smooth and shiny hair, and no abnormalities were observed in their diet and water intake. Compared with the solvent control group, there were no significant differences in the body weight gain curves of the rats in each dose group (P > 0.05). The results of blood routine and blood biochemical index tests showed that all indexes in the low, medium, and high-dose groups were within the normal reference range, and there was no significant difference compared with the solvent control group (P > 0.05). Anatomical observations found that there were no obvious abnormalities in the appearance, size, and texture of the main organs of the rats in each dose group, and there was no significant difference in the organ coefficient compared with the solvent control group (P > 0.05). The results of pathological section examinations showed that the morphological structures of the organ tissues of the rats in each dose group were normal, and no obvious pathological damage was found. In the positive control group, however, obvious weight loss, abnormal blood routine and blood biochemical indexes, and organ pathological damage occurred. For example, the body weight of the rats in the positive control group decreased by 15% on the 60th day of the experiment compared with before the experiment, the white blood cell count decreased significantly, the activities of alanine aminotransferase and aspartate aminotransferase increased significantly, and the pathological section of the liver showed hepatocyte degeneration and necrosis. By comparing with the positive control group, it was fully demonstrated that small molecule peptide P - T had no subchronic toxicity within the experimental dose range. Genotoxicity test (Ames test) Experimental strains: The histidine-deficient strains TA97, TA98, TA100, and TA102 of Salmonella typhimurium were selected as experimental strains. These strains were identified, and their biological characteristics met the experimental requirements. During the preservation and use process, they were strictly operated according to the standard operating procedures to ensure the activity and stability of the strains. The strains were regularly resuscitated and subcultured, and the growth characteristics and genetic stability of the strains were checked to ensure the reliability of the experimental results. Experimental method: The Ames test was conducted using the plate incorporation method. The small molecule peptide P - T was prepared into solutions with different concentrations using sterile water, and the concentration range was 10 - 1000 μg / plate. Three parallel plates were set for each concentration. At the same time, a positive control group (using known mutagens, such as 2 - amino - fluorene for TA97, TA98, TA100 strains, and mitomycin C for TA102 strain), a negative control group (sterile water), and a solvent control group (the solvent used, such as DMSO) were set. In the top agar, 0.1 mL of the test strain bacterial solution, 0.1 mL of the small molecule peptide solution or control solution with different concentrations, and 0.5 mL of the S9 mixture (added only when metabolic activation was required) were successively added. After quickly mixing evenly, it was poured onto the nutrient agar plate. After the top agar solidified, the plate was placed in a constant temperature incubator at 37°C for 48 hours. After the culture ended, the number of revertant mutant colonies on the plate was observed and counted. If the small molecule peptide has genetic toxicity, it will induce the strain to undergo reverse mutation, resulting in a significant increase in the number of colonies. During the experimental operation process, the addition amount and mixing time of each reagent were strictly controlled to ensure the consistency of the experimental conditions. Experimental results: The number of revertant mutant colonies in each small molecule peptide experimental group at different concentrations showed no significant difference compared with the negative control group and the solvent control group (P > 0.05), and was much lower than that of the positive control group. Even at the highest concentration of 1000 μg / plate, the small molecule peptide P - T did not induce a significant increase in the number of revertant mutant colonies in TA97, TA98, TA100, and TA102 strains. For example, at a concentration of 1000 μg / plate of the small molecule peptide P, the number of revertant mutant colonies on the TA97 strain plate was (25 ± 3), showing no significant difference (P > 0.05) compared with the negative control group (22 ± 4), while the number of revertant mutant colonies in the positive control group (2 - amino - fluorene) was (250 ± 20) under the same conditions. Through statistical analysis, it was further verified that the small molecule peptide P - T has no genetic toxicity under the experimental conditions and will not cause damage to the genetic material of organisms. Mutagenicity test (mouse bone marrow micronucleus test) Experimental animals: Healthy adult Kunming mice with a body weight of 20 - 25 g, half male and half female, were purchased from a regular experimental animal supplier. The feeding environment of the mice was the same as that in the acute toxicity test. During the feeding process of the mice, a suitable feeding environment and a nutritionally balanced diet were provided to ensure the healthy growth of the mice. Experimental grouping and administration: The mice were randomly divided into 5 groups, namely low-dose group, medium-dose group, high-dose group, negative control group and positive control group, with 10 mice in each group. The low-, medium- and high-dose groups were given the small molecule peptide P - T solution at doses of 200 mg / kg, 400 mg / kg and 800 mg / kg respectively, by intraperitoneal injection once a day for 2 consecutive days. The negative control group was given an equal volume of normal saline, and the positive control group was given cyclophosphamide at a dose of 40 mg / kg. During the administration process, the injection operation procedures were strictly followed to ensure the accuracy of the administration dose. Specimen collection and preparation: 6 hours after the last administration, the mice were sacrificed by cervical dislocation, and the bilateral femurs were taken out. A small amount of calf serum was aspirated with a syringe to rinse the bone marrow cavity, and the rinsing solution was dropped on a glass slide, smeared and air-dried, fixed with methanol for 15 minutes, stained with Giemsa stain for 15 minutes, rinsed with running water, and examined under a microscope after air-drying. During the specimen collection and preparation process, attention was paid to maintaining the standardization and consistency of the operation to reduce the influence of human factors on the experimental results. Observation and counting: Under an optical microscope, a region with uniform cell dispersion and good staining was selected, 1000 polychromatic erythrocytes (PCE) were counted, and the number of PCE containing micronuclei was observed to calculate the micronucleus rate (‰). At the same time, 200 red blood cells were counted to calculate the ratio of PCE to mature erythrocytes (NCE) to evaluate the effect of the small molecule peptide on the proliferation of bone marrow cells. During the observation and counting process, the double-blind method was adopted, and two professional personnel counted separately to reduce subjective errors. Experimental results: There was no significant difference in the micronucleus rate of polychromatic erythrocytes in the bone marrow of mice in the low-, medium- and high-dose groups compared with the negative control group (P > 0.05), and it was significantly lower than that of the positive control group. The PCE / NCE ratios in each dose group were similar to those in the negative control group, indicating that the small molecule peptide P - T had no mutagenic effect on mouse bone marrow cells and did not affect the proliferation of bone marrow cells. For example, the micronucleus rate of polychromatic erythrocytes in the bone marrow of mice in the high-dose group of small molecule peptide P was (2.5 ± 0.5)‰, that in the negative control group was (2.0 ± 0.3)‰, and that in the positive control group was (15.0 ± 2.0)‰; the PCE / NCE ratio in the high-dose group of small molecule peptide P was 0.85 ± 0.05, which was similar to 0.88 ± 0.06 in the negative control group (P > 0.05). Through the comparative analysis of the experimental data, the safety of the small molecule peptide P - T under the experimental conditions was fully demonstrated. (V) Preparation of health disinfection products Health disinfection spray Formulation Optimization: Taking small molecule peptide P as an example, the original formulation was further optimized. Each 100 mL of the spray solution contains 25 mg of small molecule peptide P, 6 mL of propylene glycol, 1.5 mL of polysorbate - 80, 0.15 g of propylparaben. Meanwhile, 0.05 g of dipotassium glycyrrhizinate was added as a soothing agent, which can effectively reduce the irritation to skin and mucous membranes. Purified water was added to make up to 100 mL. Through cell experiments and human trials, it was verified that the addition of dipotassium glycyrrhizinate does not affect the disinfection activity of small molecule peptide, and can significantly improve the comfort of product use. Improvement of Preparation Process: First, dissolve small molecule peptide P in a small amount of purified water. Under magnetic stirring, slowly add propylene glycol, polysorbate - 80 and propylparaben in sequence, and continuously stir for 30 minutes to fully dissolve and mix all components evenly. Then, add dipotassium glycyrrhizinate and continue stirring for 15 minutes. Make up the volume to 100 mL with purified water and continue stirring for 20 minutes to ensure the uniformity of solution concentration. Filter and sterilize the prepared solution through a 0.22 μm microporous membrane to ensure the sterility of the product. During the filtration process, a pre - filtration device is used to reduce the blockage of the microporous membrane and improve the filtration efficiency. Finally, divide and fill the filtered solution into spray bottles. Before encapsulation, conduct a leak - tightness test on the spray bottles to ensure no leakage during storage and use. An automated filling and packaging device is used to improve the accuracy and efficiency of filling. Enhanced Quality Control: In terms of appearance, the spray solution should be clear and transparent, without precipitation, turbidity or foreign matter. The content of small molecule peptide P in the spray was determined by high - performance liquid chromatography - tandem mass spectrometry (HPLC - MS / MS). Its content should be within the range of 90% - 110% of the labeled amount. This method has high sensitivity and selectivity and can accurately determine the content of small molecule peptide. Detect the pH value of the spray solution, which should be between 6.0 - 7.0. Within this pH range, small molecule peptide has good stability and low irritation to skin and mucous membranes. The microbial limit inspection should meet relevant standards. The total number of bacteria per milliliter of the spray solution should not exceed 100 CFU, the total number of molds and yeasts should not exceed 10 CFU, and no pathogenic bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli should be detected. In addition, test the spraying performance of the spray bottle, including spraying angle, spray particle size distribution, etc., to ensure that the spray evenly covers the target area and the spray particle size is between 50 - 150 μm to ensure good disinfection effect. A laser particle size analyzer is used to accurately measure the spray particle size and improve the accuracy of spraying performance testing. Hygienic Disinfection Wipe Formulation Optimization: Each 100g of wet wipe solution contains 30mg of small molecule peptide Q, 8g of glycerol, 2mL of polyoxyethylene sorbitan monooleate (Tween - 80), 0.2g of ethylparaben, and 1g of aloe extract is added simultaneously to enhance the moisturizing and soothing effects of the product. Purified water is added up to 100g. Through stability experiments and user feedback, it is determined that the addition of aloe extract does not affect the stability and disinfection effect of small molecule peptide Q. Improvement of Preparation Process: Dissolve small molecule peptide Q in an appropriate amount of purified water, add glycerol, Tween - 80, and ethylparaben under stirring, and stir evenly. It can be appropriately heated to 40 - 45°C to promote the dissolution of each component, but the temperature needs to be strictly controlled to avoid the denaturation of small molecule peptide. Add aloe extract and continue stirring for 10 minutes. Cut the non - woven fabric into an appropriate size (such as 18cm×22cm), soak it in the prepared wet wipe solution for 30 minutes to ensure that the non - woven fabric fully absorbs the solution and the content of small molecule peptide Q is evenly distributed. After soaking, take out the non - woven fabric, drain the excess liquid, and use heat - sealing packaging. The heat - sealing temperature is controlled at 120 - 130°C to ensure the packaging tightness, prevent water evaporation and microbial contamination. During the heat - sealing process, a temperature and pressure dual - control device is used to ensure the stability of the heat - sealing quality. Strengthening of Quality Control: In terms of appearance, the wet wipes should be flat, without wrinkles or damage, and have an appropriate degree of wetness. The ultraviolet spectrophotometry is used to determine the content of small molecule peptide Q in the wet wipes, and its content should be within the range of 93% - 107% of the labeled amount. This method is simple, rapid, and suitable for the routine detection of small molecule peptide content. Detect the pH value of the wet wipes, which should be between 5.5 - 6.5 to ensure the comfort and safety of use. The microbial limit inspection requires that the total number of bacteria per wet wipe should not exceed 20CFU, the total number of molds and yeasts should not exceed 10CFU, and pathogenic bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli should not be detected. At the same time, the moisture content of the wet wipes is detected and should be maintained between 70% - 80%, which can not only ensure the wet state of the wet wipes but also prevent microbial growth or the reduction of small molecule peptide activity caused by excessive moisture. A moisture analyzer is used to accurately detect the moisture content of the wet wipes to ensure that the moisture content meets the standards. Hygienic Disinfectant Hand Sanitizer Formulation Optimization: Each 100g of hand sanitizer contains 22mg of small molecule peptide R, 14g of sodium lauryl polyether sulfate, 6g of cocamidopropyl betaine, 10g of glycerol, an appropriate amount of citric acid (to adjust the pH value to 7.0 - 7.5), an appropriate amount of fragrance, and 0.5g of allantoin is added simultaneously, which has the effect of promoting skin repair. Purified water is added up to 100g. Through skin irritation experiments and efficacy tests, it is verified that the addition of allantoin has no adverse effect on the product performance. Improvement of Preparation Process: Dissolve sodium lauryl polyether sulfate and cocoamidopropyl betaine in an appropriate amount of purified water, heat to 65 - 70°C, stir evenly to fully dissolve the surfactant. Dissolve small molecule peptide R in a small amount of purified water and slowly add it to the above solution, then continue to stir for 25 minutes to ensure the uniform dispersion of small molecule peptide R. Add glycerol and citric acid to adjust the pH value, then add allantoin and an appropriate amount of essence, and stir evenly. Finally, add purified water to 100 g, stir evenly, and cool to room temperature. During the preparation process, strictly control the temperature and stirring speed to avoid excessive foaming affecting the product quality, and at the same time keep the production environment clean and hygienic to prevent microbial contamination. An automated temperature and stirring control system is adopted to ensure the stability of the preparation process. Enhanced Quality Control: In terms of appearance, the hand sanitizer should be a uniform milky liquid, without stratification or precipitation, and with normal color. The content of small molecule peptide R in the hand sanitizer is determined by ultra-high performance liquid chromatography (UPLC). Its content should be within the range of 95% - 105% of the labeled amount. UPLC has higher separation efficiency and analysis speed, and can more accurately determine the content of small molecule peptides. Detect the pH value of the hand sanitizer, which should be between 7.0 - 7.5, meeting the pH range of skin-friendly products. Conduct a foam performance test using a Ross-Miles foam apparatus. It is required that the initial foam height of the hand sanitizer is not less than 160 mm, and the foam height after 5 minutes is not less than 110 mm to ensure good cleaning effect. Conduct a stability test. Place the hand sanitizer at different temperatures (4°C, 25°C, 40°C) for 3 months, and observe whether there are phenomena such as stratification, color change, and taste change to evaluate the physical and chemical stability of the product. For the microbial limit inspection, the total number of bacteria per gram of hand sanitizer shall not exceed 1000 CFU, the total number of molds and yeasts shall not exceed 100 CFU, and pathogenic bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa shall not be detected to ensure the safety of the product during storage and use. An accelerated aging experiment is adopted to simulate different environmental conditions and quickly evaluate the stability of the hand sanitizer. Hygienic Disinfection Gel ** Formula Optimization Hygienic Disinfection Gel Formulation Optimization: Each 100g of the gel contains 28mg of small molecule peptide S, 1.5g of carbomer 940, 1.2g of triethanolamine, 7g of propylene glycol, 0.18g of methylparaben, and an additional 0.8g of sodium hyaluronate is added to enhance the moisturizing property and skin feel of the gel. Sodium hyaluronate has a powerful moisturizing ability and can form a moisturizing film on the skin surface, which not only enhances the moisturizing effect of the product but also improves the skin permeability of small molecule peptide S, enabling it to better exert its disinfection function. Through in vitro transdermal experiments and feedback from volunteer trials, it is confirmed that after adding sodium hyaluronate, the penetration amounts of small molecule peptide S in the stratum corneum and epidermis of the skin increase by 20% and 15% respectively, and users generally report that the skin is more moisturized and comfortable after using the gel, and no adverse reactions occur. Improvement of Preparation Process: First, slowly add carbomer 940 to an appropriate amount of purified water and stir with a high-speed stirrer at a speed of 1500r / min for 30 minutes to allow it to fully swell and form a uniform dispersion. During this process, monitor the solution viscosity in real time through an on-line viscosity monitor to ensure that carbomer 940 is fully swollen. Dissolve small molecule peptide S in a small amount of purified water and add it to the above dispersion, and continue stirring for 25 minutes. Adjust the pH value to 6.8 - 7.2 with triethanolamine, and monitor the pH value change in real time using a high-precision pH electrode during the adjustment process to gradually form a gel-like solution. Add propylene glycol and methylparaben and stir evenly. Finally, add sodium hyaluronate and stir at a speed of 800r / min for 15 minutes to ensure its uniform dispersion in the gel system. Make up the volume to 100g with purified water, stir evenly, and then dispense it into gel tubes. In the dispensing process, use a fully automatic gel filling machine with a filling accuracy of up to ±0.05g, which greatly improves the accuracy and efficiency of dispensing and reduces the risk of product contamination at the same time. Enhanced Quality Control: In terms of appearance, the gel should be in a uniform and delicate semi-solid state without particles or foreign matters. The content of small molecule peptide S in the gel is determined by high performance liquid chromatography (HPLC), and the content should be within the range of 92% - 108% of the labeled amount. During the HPLC detection process, parameters such as the selection of the chromatographic column, the ratio of the mobile phase, and the detection wavelength are optimized to ensure the accuracy and repeatability of the detection results. The pH value of the gel is detected and should be between 6.8 - 7.2 to ensure the stability of the gel and its safety for the skin. For the microbial limit inspection, the total number of bacteria per gram of the gel shall not exceed 100 CFU, the total number of molds and yeasts shall not exceed 10 CFU, and pathogenic bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa shall not be detected. Stability testing is carried out by placing the gel at different temperatures (4°C, 25°C, 40°C) for 3 months, and at the same time, an accelerated stability experiment is carried out by placing it under the conditions of a temperature of 50°C and a relative humidity of 75% for 2 weeks. The stability of the product is comprehensively evaluated by observing whether the morphology, color, and odor of the gel change, and whether the content of small molecule peptide S is within the specified range. During the stability testing process, techniques such as differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) are used to deeply analyze the physical and chemical changes of the gel under different conditions, providing more comprehensive data support for the quality control of the product.

Claims

1. A small molecule peptide combination for plant-derived hygiene disinfection, characterized in that, It contains the following 5 small molecule peptides: Small molecule peptide P: Gly - Leu - Gln - His - Asp - Pro - Lys - Cys - Ile - Ser - Tyr - Asn - Glu - Val - Thr (GLQHDPRCISYNEVT); Small molecule peptide Q: His - Ala - Ser - Cys - Glu - Trp - Pro - Gly - Lys - Val - Tyr - Asp - Cys - Leu - Met (HASCEWPGKVYDCLM); Small molecule peptide R: Ala - Cys - His - Lys - Asp - Pro - Ile - Leu - Ser - Tyr - Asn - Glu - Val - Met - Thr (ACHKDPI LSYNEVMT); Small molecule peptide S: Thr - Glu - Asp - Cys - His - Pro - Arg - Gly - Ile - Val - Leu - Ser - Phe - Tyr - Lys (TEDCHPR GIVLSFYK); Small molecule peptide T: Val - Pro - Cys - Lys - Trp - Asp - His - Ile - Gly - Leu - Met - Thr - Phe - Asn - Glu (VPCKW DHI GLMTFNE).

2. The extraction method of the small molecule peptide combination according to claim 1, characterized in that It includes the following steps: Raw material pretreatment: Select the whole herb of Andrographis paniculata and the rhizome of Isatis indigotica, wash, dry, and pulverize them into powder; Mixed extraction: Mix the Andrographis paniculata and Isatis indigotica powder in a mass ratio of 3:2, add a 10-fold volume of 70% ethanol-water mixed solution, and perform ultrasonic-assisted reflux extraction at 60 °C for 3 times, 2 hours each time. Combine the extraction solutions and concentrate them under reduced pressure; Macroporous resin adsorption and separation: Pass the concentrated solution through an AB-8 macroporous adsorption resin column, elute successively with deionized water and 45% ethanol solution, and collect the eluate; - Enzymatic reaction: Adjust the pH of the eluate to 7.5, add a mixed enzyme solution of trypsin and neutral protease, and perform oscillating enzymatic hydrolysis at 45 °C for 6 hours; Ultrafiltration purification: Ultrafilter the enzymatic hydrolysis solution through a ultrafiltration membrane with a molecular weight cut-off of 3000 Da, and collect the permeate; Ion exchange chromatography: Load the permeate onto a DEAE-Sepharose Fast Flow weak anion exchange resin column, first rinse with 0.03M Tris-HCl buffer solution, and then elute with a gradient of 0-0.5M sodium chloride solution, and collect the elution peak with disinfection activity; - Gel filtration chromatography: Load the elution peak onto a Sephadex G-25 gel column, elute with 0.1M phosphate buffer solution, and collect the target small molecule peptide fraction; Freeze-drying: Freeze-dry the collected small molecule peptide fraction to obtain small molecule peptide dry powder.

3. Use of the small molecule peptide combination according to claim 1 in a hygiene disinfection product, characterized in that, The sanitary disinfection products include, but are not limited to, sanitary disinfection sprays, sanitary disinfection wet wipes, sanitary disinfection hand sanitizers, and sanitary disinfection gels.

4. The application according to claim 3, wherein: The formula of the sanitary disinfection spray is 25 mg of small molecule peptide P, 6 mL of propylene glycol, 1.5 mL of polysorbate-80, 0.15 g of propyl p-hydroxybenzoate per 100 mL, and purified water is added to 100 mL; - The formula of the sanitary disinfection wet wipe is 30 mg of small molecule peptide Q, 8 g of glycerol, 2 mL of polyoxyethylene sorbitan monooleate, 0.2 g of ethylparaben per 100 g, and purified water is added to 100 g; The formula of the sanitary disinfection hand sanitizer is 22 mg of small molecule peptide R, 14 g of sodium lauryl polyether sulfate, 6 g of cocamidopropyl betaine, 10 g of glycerol, appropriate amount of citric acid, appropriate amount of essence per 100 g, and purified water is added to 100 g; - The formula of the sanitary disinfection gel is 28 mg of small molecule peptide S, 1.5 g of carbomer 940, 1.2 g of triethanolamine, 7 g of propylene glycol, 0.18 g of methyl p-hydroxybenzoate per 100 g, and purified water is added to 100 g.

5. The small molecule peptide combination according to claim 1, wherein The small molecule peptide exerts a sanitary disinfection effect by destroying the cell membrane structure of pathogens, interfering with the energy metabolism of pathogens, and inhibiting the biosynthesis of pathogens.

6. A sanitary disinfection product, characterized in that, It contains the small molecule peptide combination described in claim 1 and a pharmaceutically acceptable carrier or excipient.

7. The hygiene and disinfection product according to claim 6, wherein, The product dosage form is liquid, semi-solid or solid.

8. The hygiene disinfection product according to claim 7, characterized in that: The liquid dosage form includes solution, suspension, emulsion; - The semi-solid dosage form includes gel, ointment; - The solid dosage form includes tablet, capsule, powder.

9. The small molecule peptide combination, extraction method, application or health disinfection product according to any one of claims 1 - 8, characterized in that The small molecule peptide combination is used to kill or inhibit pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, influenza virus, respiratory syncytial virus, and herpes simplex virus type 1.

10. The extraction method according to claim 2, wherein In the raw material pretreatment step, the origin, harvesting season, and growth environment of Andrographis paniculata and Isatis indigotica are comprehensively considered to ensure the stability and consistency of the raw material quality; in the mixed extraction step, an online monitoring device is used to monitor the extraction temperature, ultrasonic power, and extraction time in real time; in the macroporous resin adsorption and separation step, parameters such as the sample loading flow rate, eluent concentration, and elution volume are optimized through dynamic adsorption experiments; in the enzymatic hydrolysis reaction step, high performance liquid chromatography is used to monitor the changes in peptide segments during the enzymatic hydrolysis process in real time; in the ultrafiltration purification step, a membrane flux monitor is used to monitor the flux changes of the ultrafiltration membrane in real time; in the ion exchange chromatography step, efficient separation of small molecule peptides is achieved by optimizing the elution gradient, flow rate, and buffer composition; in the gel filtration chromatography step, the eluent flow rate is ensured to be stable and an automatic fraction collector is used to accurately collect the eluent; in the freeze-drying step, a vacuum freeze dryer is used and the freezing temperature, vacuum degree, and drying time parameters are monitored in real time.