Low-temperature biological sample preservation device

Abstract

The invention discloses a low-temperature biological sample preservation device, and relates to the technical field of biological sample preservation, the low-temperature biological sample preservation device comprises a heat preservation box, the heat preservation box is connected with a refrigerant conveying device, the top of the heat preservation box is provided with a pick-and-place pipe communicated with the interior of the heat preservation box, and the top of the heat preservation box is provided with a first motor and a second motor through a support; a pipe cover is movably mounted at a pipe opening of the pick-and-place pipe; a main shaft is vertically and rotationally installed in the heat preservation box, the top end of the main shaft is connected with a driving shaft of the first motor, a plurality of fixing rods are fixed to the bottom of the main shaft, and a gear ring is installed through the fixing rods; a driving shaft of the second motor extends into the heat preservation box and is in transmission connection with the top of the sleeve through a transmission assembly. According to the low-temperature storage device, large-batch storage and low-temperature storage of the biological samples can be achieved, the operation process is simple and convenient, the safety of the biological samples is guaranteed during operation, and efficient and safe low-temperature storage of the biological samples is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample preservation, and in particular to a low-temperature biological sample preservation device. Background Art

[0002] Biological samples are important resources for biological research. In order to ensure the activity of biological samples, they need to be stored in a deep low temperature environment as much as possible. The research and development of modern biomedicine cannot be separated from biological cryopreservation technology. Cryopreservation can inhibit the biochemical activity of biological samples and reduce their metabolic rate, so that biological samples can be preserved for a long time. The lower the storage temperature, the longer the storage time. Experiments have shown that after biological cells were cryopreserved at -196°C liquid nitrogen for many years, no biochemical and functional mutations were found in the biological cells after rewarming. Therefore, biological cryopreservation technology can achieve long-term and safe preservation of biological samples, and has broad prospects in the field of preservation of genetic material and its germplasm resources.

[0003] After searching, Chinese patent application No. 202022945237.0 discloses a biological sample low-temperature preservation device, including a preservation tank, a driving component, a lifting component and a sample carrier. The preservation tank has an insulation chamber inside and a movable guide rail is vertically arranged in the insulation chamber. The driving component is arranged on the top of the preservation tank. The sample carrier is arranged in the insulation chamber and its two sides are slidably connected to the movable guide rail through a sliding component. One end of the lifting component is transmission connected to the driving component, and the other end is fixed to the top of the sample carrier. The driving component is driven by the lifting component to make the sample carrier reciprocate on the movable guide rail.

[0004] The prior art has the following deficiencies: the existing biological sample storage device cannot store multiple sample carriers in a storage tank, which limits the actual scope of use and is not suitable for large-scale biological sample storage operations. Therefore, the present invention proposes a low-temperature biological sample storage device. Summary of the invention

[0005] The purpose of the present invention is to solve the defects in the prior art and to propose a low-temperature biological sample storage device.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A low-temperature biological sample storage device comprises an incubator, the incubator is connected to a refrigerant conveying device, a pick-up and release tube connected to the interior of the incubator is installed on the top of the incubator, a first motor and a second motor are installed on the top of the incubator through a bracket, and a tube cover is movably installed at the tube mouth of the pick-up and release tube;

[0008] A main shaft is vertically and rotatably installed inside the incubator. The top end of the main shaft is connected to the drive shaft of the first motor. Multiple fixing rods are fixed to the bottom of the main shaft, and a toothed ring is installed through the fixing rods. A sleeve is sleeved on the upper part of the main shaft. The drive shaft of the second motor extends into the incubator and is in transmission connection with the top of the sleeve through a transmission component. The top of the sleeve is rotatably installed with the inner top wall of the incubator.

[0009] A plurality of sector-shaped wire frames are fixedly arranged at equal intervals and in a circular shape at the bottom of the sleeve. Storage boxes are placed on the sector-shaped wire frames.

[0010] A vertical guide rail is fixed to one side of the incubator, and a fixed plate and a movable plate are slidably arranged through the vertical guide rail.

[0011] A wire sleeve is fixed to the bottom wall of the pipe cover. The fixed plate is fixed to the bottom of the wire sleeve. The wire sleeve is threadedly installed with a lead screw. The lead screw is vertically and rotatably installed on one side inside the incubator. A gear is fixedly installed at the lower end of the lead screw. The gear is in meshing transmission with the toothed ring.

[0012] The movable plate is slidably installed on the surface of the wire sleeve. The movable plate is located above the fixed plate. A sliding hole matching the wire sleeve is formed in the front side of the plate body of the movable plate. A spring is installed above the sliding hole. A limiting ring is fixed to the wire sleeve. The limiting ring is located above the sliding hole and the spring.

[0013] Further, the storage box includes a box body and a box cover. A rotating shaft is horizontally and rotatably installed inside the box body near the lower position. One end of the rotating shaft extends to the outside of the box body and a knob is installed. Two driving bevel gears are installed on the rotating shaft. A plurality of placing racks are fixed inside the box body through two vertically arranged threaded pipes. The top of the threaded pipe is fixedly installed with the bottom surface of the box cover. A threaded rod is threadedly installed below the threaded pipe. The threaded rod is connected to the inner side wall of the box body through a rotatably installed support seat. A driven bevel gear is installed at the bottom end of the threaded rod. The driving bevel gear is in meshing transmission with the driven bevel gear.

[0014] Further, a slider is fixed to the side surface of the lowermost placing rack. A sliding groove matching the slider is vertically formed in the inner side wall of the box body. The height of the upper end of the sliding groove is lower than the position of the box opening of the box body.

[0015] Further, an arc-shaped plate is fixed to the rear side of the plate body of the movable plate. The center of the bottom of the arc-shaped plate is a planar structure, and both sides of the bottom of the arc-shaped plate are arc-shaped structures. The bottom of the arc-shaped plate is in sliding contact with the top surface of the box cover.

[0016] Further, a fence is fixed to the sector-shaped wire frame. A through groove is formed in the bottom plate of the sector-shaped wire frame at the center of the fence. The size of the through groove matches the front end of the fixed plate, and the size of the through groove is smaller than the bottom size of the storage box.

[0017] Furthermore, a plurality of clamping components are provided above the sector-shaped grid frame and outside the enclosure. The clamping component includes a U-shaped seat fixed above the sector-shaped grid frame. A central shaft is rotatably installed at the center of the U-shaped seat. An oscillating arm is vertically fixed in the middle of the central shaft. Torsion springs are installed on both sides of the bottom end of the oscillating arm on the central shaft. A pulley is installed at the upper end of the oscillating arm.

[0018] Furthermore, a baffle is fixed on one side of the U-shaped seat close to the center of the enclosure.

[0019] Furthermore, the transmission component includes a shaft rod. The two ends of the shaft rod are rotatably installed at the lower end of the hanging plate. The upper end of the hanging plate is fixed on the inner top wall of the incubator. A worm is installed on the shaft rod. A first bevel gear is installed on the driving shaft of the second motor. A second bevel gear is installed at one end of the shaft rod. The first bevel gear and the second bevel gear are meshed and driven. A slip ring and a worm gear are fixed on the outer side of the top end of the sleeve. The worm is meshed and driven with the worm gear. A retaining ring is fixed on the inner top wall of the incubator. The retaining ring is slidably sleeved on the outer side of the slip ring. The top end of the sleeve is rotatably installed on the inner top wall of the incubator through the slip ring and the retaining ring.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] 1. In the present invention, a plurality of sector-shaped grid frames distributed in a circumferential manner are arranged in the incubator. The number of storage boxes containing biological samples can be multiple, and the multiple storage boxes are stably placed on the sector-shaped grid frames. Subsequently, low-temperature preservation can be conveniently achieved by using a refrigerant.

[0022] 2. In the present invention, the operation of taking and placing the storage box is carried out by the cooperation of the liftable fixed plate and the movable plate. During the operation process, the fixed plate cooperates with the movable plate to apply a clamping force to the storage box, so that the storage box is very convenient and stable during the storage process, thereby efficiently and conveniently putting the biological sample into the incubator for low-temperature storage.

[0023] 3. In the present invention, a plurality of placement racks are arranged inside the storage box, and more biological samples can be stored in layers. Moreover, after the biological samples are placed, they can be quickly isolated to avoid being contaminated during the placement process.

[0024] 4. When the storage box is placed on the sector-shaped grid frame, it is clamped and positioned by using the enclosure and the clamping component in cooperation, so that the stability of the storage box can be ensured when the sector-shaped grid frame rotates, and the internal biological samples are not affected.

[0025] In summary, the present invention can achieve the mass storage and low-temperature preservation of biological samples. The operation process is simple and convenient, and the safety of biological samples is ensured during the operation, realizing the efficient and safe low-temperature preservation of biological samples. Description of the Drawings

[0026] The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention.

[0027] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 is an internal sectional view of the present invention;

[0029] Figure 3 is Figure 2 a schematic diagram when the storage box is moved to the nozzle of the pick-up and placement tube in

[0030] Figure 4 is a schematic diagram of the cooperation of the fixing plate, the interaction plate, the storage box and the sector-shaped wire frame;

[0031] Figure 5 is Figure 3 an enlarged view of the structure of part a in

[0032] Figure 6 is Figure 3 an enlarged view of the structure of part b in

[0033] Figure 7 is Figure 3 an enlarged view of the structure of part c in

[0034] Figure 8 is a schematic diagram of the structure of the storage box;

[0035] Figure 9 is an internal sectional view of the storage box;

[0036] Figure 10 is a schematic diagram of the installation between the placement racks and the threaded tube;

[0037] Figure 11 is Figure 9 an enlarged view of the structure of part d in

[0038] Figure 12 is a schematic diagram of the structure of the movable plate;

[0039] Figure 13 is a schematic diagram of the structure of the clamping assembly.

[0040] In the figure: 1 insulation box, 2 pick-up and placement tube, 3 tube cap, 4 support, 5 first motor, 6 second motor, 7 main shaft, 8 sleeve, 9 transmission assembly, 10 fixed rod, 11 gear ring, 12 gear, 13 lead screw, 14 lead screw sleeve, 15 fixing plate, 16 movable plate, 17 storage box, 18 sector-shaped wire frame, 19 clamping assembly, 20 vertical guide rail, 21 spring, 22 limit ring;

[0041] 81 Slip ring, 82 retaining ring; 91 bevel gear one, 92 bevel gear two, 93 shaft rod, 94 worm, 95 worm gear; 161 arc-shaped plate; 171 box body, 172 box cover, 173 rotating shaft, 174 placing rack, 175 threaded pipe, 176 threaded rod, 177 driving bevel gear, 178 driven bevel gear, 179 slider; 181 enclosure, 182 through slot, 183 mesh hole; 191 U-shaped seat, 192 central shaft, 193 swing arm, 194 pulley, 195 torsion spring, 196 baffle plate. Detailed implementation manner

[0042] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention;

[0043] Refer to Figures 1-13 , a cryogenic biological sample preservation device, including a heat preservation box 1, the heat preservation box 1 is connected with a refrigerant delivery device, and the heat preservation box 1 can also be internally provided with a temperature sensor to facilitate monitoring whether the internal temperature meets the preservation requirements. A pick-up and delivery pipe 2 communicating with the inside thereof is installed on the top of the heat preservation box 1, a first motor 5 and a second motor 6 are installed on the top of the heat preservation box 1 through a bracket 4, and a pipe cover 3 is movably installed at the pipe orifice of the pick-up and delivery pipe 2;

[0044] A main shaft 7 is vertically rotatably installed inside the heat preservation box 1, the top end of the main shaft 7 is connected to the drive shaft of the first motor 5, a plurality of fixing rods 10 are fixed to the bottom of the main shaft 7, and a gear ring 11 is installed through the fixing rods 10. A sleeve 8 is sleeved on the upper part of the main shaft 7, and the drive shaft of the second motor 6 extends into the heat preservation box 1 and is in transmission connection with the top of the sleeve 8 through a transmission assembly 9; the top of the sleeve 8 is rotatably installed with the inner top wall of the heat preservation box 1;

[0045] A plurality of sector-shaped wire frames 18 are equidistantly and circumferentially fixed to the bottom of the sleeve 8, and storage boxes 17 are placed on the sector-shaped wire frames 18;

[0046] A vertical guide rail 20 is fixed to one side of the heat preservation box 1, and a fixing plate 15 and a movable plate 16 are slidably arranged through the vertical guide rail 20;

[0047] A silk sleeve 14 is fixed to the bottom wall of the pipe cover 3, the fixing plate 15 is fixed to the bottom of the silk sleeve 14, the silk sleeve 14 is threadedly installed with a lead screw 13, the lead screw 13 is vertically and rotatably installed on one side inside the heat preservation box 1, a gear 12 is fixedly installed at the lower end of the lead screw 13, and the gear 12 is in meshing transmission with the gear ring 11;

[0048] The movable plate 16 is slidably installed on the surface of the silk sleeve 14, and the movable plate 16 is located above the fixing plate 15. A sliding hole matching the silk sleeve 14 is formed in the front side of the plate body of the movable plate 16, and a spring 21 is installed above the sliding hole. A limit ring 22 is fixed to the silk sleeve 14, and the limit ring 22 is located above the sliding hole and the spring 21.

[0049] Furthermore, the transmission assembly 9 includes a shaft rod 93. Both ends of the shaft rod 93 are rotatably installed at the lower end of the hanging plate, and the upper end of the hanging plate is fixed to the inner top wall of the incubator 1. A worm 94 is installed on the shaft rod 93. A first bevel gear 91 is installed on the drive shaft of the second motor 6. A second bevel gear 92 is installed at one end of the shaft rod 93. The first bevel gear 91 and the second bevel gear 92 are meshed and driven. A slip ring 81 and a worm gear 95 are fixed to the outer side of the top end of the sleeve 8. The worm 94 and the worm gear 95 are meshed and driven. A retaining ring 82 is fixed to the inner top wall of the incubator 1. The retaining ring 82 is slidably sleeved on the outer side of the slip ring 81. The top end of the sleeve 8 is rotatably installed on the inner top wall of the incubator 1 through the slip ring 81 and the retaining ring 82.

[0050] When the second motor 6 is driven, the rotation of the shaft rod 83 can be controlled by the meshing transmission of the first bevel gear 91 and the second bevel gear 92. The meshing transmission of the worm 94 on the shaft rod 93 and the worm gear 95 can control the rotation of the sleeve 8 and the lower fan-shaped grid 18 relative to the main shaft 7, so as to switch the positions of each storage box 17, facilitating the subsequent storage and retrieval operations of biological samples.

[0051] Furthermore, the storage box 17 includes a box body 171 and a box cover 172. A rotating shaft 173 is horizontally rotatably installed inside the box body 171 near the lower position. One end of the rotating shaft 173 extends to the outside of the box body 171 and a knob is installed. Two driving bevel gears 177 are installed on the rotating shaft 173. A plurality of placement racks 174 are fixed inside the box body 171 through two vertically arranged threaded tubes 175. The top of the threaded tube 175 is fixedly installed with the bottom surface of the box cover 172. A threaded rod 176 is threadedly installed below the threaded tube 175, and the threaded rod 176 is connected to the inner side wall of the box body 171 through a rotatably installed support seat. A driven bevel gear 178 is installed at the bottom end of the threaded rod 176. The driving bevel gear 177 and the driven bevel gear 178 are meshed and driven.

[0052] When the box cover 172 is opened, biological samples can be first placed on the placement rack 174 inside the box body 171. When placing the biological samples, it is decided whether a separate container is needed according to requirements; after storage, the box cover 172 is closed, and then the whole storage box 17 is put into the incubator 1 from the pipe orifice of the access pipe 2. When placing, the storage box 17 is placed on the fixed plate 15. The movable plate 16 is pushed upward. At this time, the spring 21 is compressed, and its elasticity is used to prompt the movable plate 16 to cooperate with the fixed plate 15 to apply a vertical clamping force to the whole storage box 17, so as to ensure that the storage box 17 can remain stable when placed inside the incubator 1 and ensure the safety of the internal biological samples.

[0053] Among them, the rotation of the rotating shaft 173 is controlled by a knob. Under the meshing transmission cooperation of the driving bevel gear 177 and the driven bevel gear 178, the rotation of the threaded rod 176 is controlled. Then, in cooperation with the threaded fit between the threaded rod 176 and the threaded tube 175, the lifting of the placement rack 174 and the box cover 172 can be controlled. When descending, the box cover 172 can be used to close the box body 171, and after closing, it can prevent the external environment from affecting the internal biological samples.

[0054] Further, a slider 179 is fixed on the side of the lowermost placement rack 174. A chute matching the slider 179 is vertically opened on the inner side wall of the box body 171, and the upper end of the chute is at a position lower than the box opening of the box body 171 in height. The slider 179 and the chute match each other, which can ensure the stability of the placement rack 174 during lifting and lowering. And the height design of the chute makes the lowermost placement rack 174 not leave the box body 171, avoiding accidental spillage of biological samples when taking and placing biological samples.

[0055] Further, an arc-shaped plate 161 is fixed to the rear side of the plate body of the movable plate 16. The center of the bottom of the arc-shaped plate 161 is a planar structure, and the two sides of the bottom of the arc-shaped plate 161 are arc-shaped structures. The bottom of the arc-shaped plate 161 is in sliding contact with the top surface of the box cover 172.

[0056] The sleeve 8 is connected to the drive shaft of the second motor 6 by a transmission component 9. Therefore, when the second motor 6 is driven, the sleeve 8 and the multiple sector-shaped wire frames 18 can be controlled to rotate circumferentially around the main shaft 7 at the same time. At this time, the storage boxes 17 placed at other positions can be rotated to the lower side of the movable plate 16, and moreover, the top surface of the box cover 172 can squeeze the bottom of the arc-shaped plate 161, so that the box cover 172 gradually comes to be directly below the arc-shaped plate 161, enabling the storage boxes 17 at different positions to enter between the movable plate 16 and the fixed plate 15 during rotation and thus be clamped. Then, when the fixed plate 15 and the movable plate 16 rise later, the different storage boxes 17 on different sector-shaped wire frames 18 can be taken out in sequence. Similarly, biological samples can also be placed in different storage boxes 17 for storage.

[0057] Further, a retaining wall 181 is fixed on the sector-shaped wire frame 18. A through slot 182 is opened on the bottom plate of the sector-shaped wire frame 18 at the center of the retaining wall 181. The size of the through slot 18 is matched with the front end of the fixed plate 15, and the size of the through slot 18 is smaller than the bottom size of the storage box 17.

[0058] Furthermore, a plurality of clamping components 19 are provided above the fan-shaped grid frame 18 and outside the enclosure 181. The clamping component 19 includes a U-shaped seat 191 fixed above the fan-shaped grid frame 18. A central shaft 192 is rotatably installed at the center of the U-shaped seat 191. A swing arm 193 is vertically fixed in the middle of the central shaft 192. Torsion springs 195 are installed on both sides of the central shaft 192 at the bottom end of the swing arm 193. A pulley 194 is installed at the upper end of the swing arm 193.

[0059] The clamping component 9 can apply a clamping force to the side surface of the storage box 17. The clamping force is approximately a horizontally applied force, which can make the storage box 17 stable when rotating with the fan-shaped grid frame 18, and can also facilitate the storage box 17 to enter or leave between the fixed plate 15 and the movable plate 16 more conveniently, while ensuring that the internal biological samples will not be poured.

[0060] Furthermore, a baffle 196 is fixed on one side of the U-shaped seat 191 close to the center of the enclosure 181. The presence of the baffle 196 can prevent the pulley and the swing arm 193 from getting too close to the center of the enclosure 181, thus ensuring that there is no restriction when putting the storage box 17 inside.

Claims

1. A cryogenic biological sample preservation device, comprising a heat preservation box (1), and the heat preservation box (1) is connected with a refrigerant conveying device, characterized in that, A pick-up and placement pipe (2) communicating with its interior is installed at the top of the incubator (1). A first motor (5) and a second motor (6) are installed at the top of the incubator (1) through a bracket (4). A pipe cover (3) is movably installed at the orifice of the pick-up and placement pipe (2). A main shaft (7) is vertically rotatably installed inside the incubator (1). The top end of the main shaft (7) is connected to the drive shaft of the first motor (5). A plurality of fixing rods (10) are fixed to the bottom of the main shaft (7), and a toothed ring (11) is installed through the fixing rods (10). A sleeve (8) is sleeved on the upper part of the main shaft (7). The drive shaft of the second motor (6) extends into the incubator (1) and is in transmission connection with the top of the sleeve (8) through a transmission component (9). The top of the sleeve (8) is rotatably installed with the inner top wall of the incubator (1). A plurality of sector-shaped wire frames (18) are fixed at equal intervals and circumferentially at the bottom of the sleeve (8). Storage boxes (17) are placed on the sector-shaped wire frames (18). A vertical guide rail (20) is fixed to one side of the incubator (1), and a fixing plate (15) and a movable plate (16) are slidably arranged through the vertical guide rail (20). A wire sleeve (14) is fixed to the bottom wall of the pipe cover (3). The fixing plate (15) is fixed to the bottom of the wire sleeve (14). The wire sleeve (14) is threadedly installed with a lead screw (13). The lead screw (13) is vertically and rotatably installed on one side inside the incubator (1). A gear (12) is fixedly installed at the lower end of the lead screw (13). The gear (12) is in meshing transmission with the toothed ring (11). The movable plate (16) is slidably installed on the surface of the wire sleeve (14), and the movable plate (16) is located above the fixing plate (15). A sliding hole matching the wire sleeve (14) is formed in the front side of the plate body of the movable plate (16), and a spring (21) is installed above the sliding hole. A limiting ring (22) is fixed to the wire sleeve (14), and the limiting ring (22) is located above the sliding hole and the spring (21).

2. The cryogenic biological sample preservation device according to claim 1, wherein, The storage box (17) includes a box body (171) and a box cover (172). A rotating shaft (173) is horizontally rotatably installed inside the box body (171) and near the lower position. One end of the rotating shaft (173) extends to the outside of the box body (171) and is installed with a knob. Two driving bevel gears (177) are installed on the rotating shaft (173). A plurality of placing racks (174) are fixed inside the box body (171) through two vertically arranged threaded pipes (175). The top of the threaded pipe (175) is fixedly installed with the bottom surface of the box cover (172). A threaded rod (176) is threadedly installed below the threaded pipe (175), and the threaded rod (176) is connected to the inner side wall of the box body (171) through a rotatably installed support seat. A driven bevel gear (178) is installed at the bottom end of the threaded rod (176). The driving bevel gear (177) is in meshing transmission with the driven bevel gear (178).

3. The cryogenic biological sample preservation device according to claim 2, characterized in that, A slider (179) is fixed to the side of one of the placement racks (174) located at the lowermost side. A chute matching the slider (179) is vertically formed on the inner side wall of the box body (171), and the height where the upper end of the chute is located is lower than the position of the box opening of the box body (171).

4. A cryogenic biological sample preservation device according to claim 3, characterized in that, An arc-shaped plate (161) is fixed to the rear side of the plate body of the movable plate (16). The center of the bottom of the arc-shaped plate (161) is a flat structure, and both sides of the bottom of the arc-shaped plate (161) are arc-shaped structures. The bottom of the arc-shaped plate (161) is in sliding contact with the top surface of the box cover (172).

5. A cryogenic biological sample preservation device according to any one of claims 1-4, characterized in that, A retaining wall (181) is fixed to the fan-shaped wire frame (18). A through slot (182) is formed on the bottom plate of the fan-shaped wire frame (18) at the center of the retaining wall (181). The size of the through slot (18) matches the front end of the fixing plate (15), and the size of the through slot (18) is smaller than the bottom size of the storage box (17).

6. The cryogenic biological sample storage device according to claim 5, wherein, Above the fan-shaped wire frame (18) and outside the retaining wall (181), a plurality of clamping assemblies (19) are provided. The clamping assembly (19) includes a U-shaped seat (191) fixed above the fan-shaped wire frame (18). A central shaft (192) is rotatably installed at the center of the U-shaped seat (191). An oscillating arm (193) is vertically fixed to the middle of the central shaft (192). Torsion springs (195) are installed on both sides of the central shaft (192) at the bottom end of the oscillating arm (193). A pulley (194) is installed at the upper end of the oscillating arm (193).

7. The cryogenic biological sample preservation device according to claim 6, wherein, A baffle (196) is fixed to one side of the U-shaped seat (191) close to the center of the retaining wall (181).

8. The cryogenic biological sample preservation device according to claim 1, characterized in that, The transmission assembly (9) includes a shaft rod (93). Both ends of the shaft rod (93) are rotatably installed at the lower end of the hanging plate. The upper end of the hanging plate is fixed to the inner top wall of the incubator (1). A worm (94) is installed on the shaft rod (93). A first bevel gear (91) is installed on the drive shaft of the second motor (6). A second bevel gear (92) is installed at one end of the shaft rod (93). The first bevel gear (91) and the second bevel gear (92) are in meshing transmission. A slip ring (81) and a worm gear (95) are fixed to the outer side of the top end of the sleeve (8). The worm (94) and the worm gear (95) are in meshing transmission. A retaining ring (82) is fixed to the inner top wall of the incubator (1). The retaining ring (82) is slidably sleeved on the outer side of the slip ring (81). The top end of the sleeve (8) is rotatably installed on the inner top wall of the incubator (1) through the slip ring (81) and the retaining ring (82).

Images