Composition for improving salt tolerance of plants and application thereof

Abstract

The invention discloses a composition for improving salt tolerance of plants and application of the composition. The composition is prepared from gallic acid and p-coumaric acid according to the mass ratio of 1: (1-4). The invention provides application of a composition of gallic acid and p-coumaric acid in promoting plant growth and improving plant salt tolerance, gallic acid and p-coumaric acid are two natural phenolic compounds, are widely distributed in plant secondary metabolites, and can reduce salt damage, improve the tender leaf length and root length of plants and improve the salt tolerance of the plants when applied under the salt stress condition. The purposes of protecting and strengthening seedlings and improving the salt stress resistance of plants can be achieved in production.

Description

Technical Field

[0001] The present invention relates to the technical field of crop cultivation, and specifically relates to a composition for improving plant salt tolerance and its application. Background Art

[0002] Soil salinization is a major challenge in agricultural production, which is mainly manifested as excessive salt content in the soil, resulting in crops being unable to normally absorb water and nutrients, and thus causing a significant decline in yield and quality. At present, salinization has become one of the key factors restricting the sustainable development of agriculture. The global salinized land area accounts for about 7% of the arable land area, especially serious in arid and semi-arid regions. How to improve the tolerance of crops under salt stress is a hot topic in agricultural science and biotechnology research.

[0003] To cope with salt stress, plants improve their salt tolerance through various physiological and biochemical pathways, such as regulating osmotic balance (synthesizing osmotic adjustment substances such as proline and betaine), scavenging reactive oxygen species (enhancing antioxidant enzyme activity), and regulating ion balance (regulating ion distribution through Na + / K + ion transporters). However, in the natural environment, the self-regulation ability of plants is often insufficient to resist high-concentration salt stress, so external means are needed to enhance the salt resistance of plants. At present, the external means to improve plant salt tolerance mainly include: enhancing plant salt tolerance by spraying or irrigating plant growth hormones (such as abscisic acid, gibberellin, etc.) or osmotic adjustment substances (such as mannitol, proline); or applying reactive oxygen species scavengers (such as ascorbic acid, glutathione) to alleviate oxidative damage in salt stress, but these antioxidants are easily degraded and have poor stability, making it difficult to play a long-term role in the actual field environment.

[0004] In recent years, some natural plant extracts have gradually attracted attention due to their good biological activity and environmental friendliness. For example, phenolic compounds are considered to have potential in improving plant salt tolerance due to their excellent antioxidant properties and versatility. Gallic acid and p-coumaric acid are two naturally occurring phenolic compounds, widely distributed in plant secondary metabolites, and have good antioxidant, antibacterial and anti-stress abilities. There is currently no report on using gallic acid and p-coumaric acid as a composition to improve the salt tolerance of crops. Summary of the Invention

[0005] Aiming at the above-mentioned existing technologies, the purpose of the present invention is to provide a composition for improving plant salt tolerance and its application.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions:

[0007] In the first aspect of the present invention, a composition for improving the salt tolerance of plants is provided, and the composition is composed of gallic acid and p-coumaric acid in a mass ratio of 1:(1 - 4).

[0008] In the second aspect of the present invention, an application of the above composition in at least one of the following (1)-(2) is provided:

[0009] (1) Improving the salt tolerance of plants;

[0010] (2) Preparing a product for improving the salt tolerance of plants.

[0011] Preferably, the improvement of the salt tolerance of plants is to improve the germination rate of seeds, the length of young leaves of seedlings, and the root length of seedlings under salt stress.

[0012] Preferably, the plant is wheat or rice.

[0013] In the third aspect of the present invention, a method for improving the salt tolerance of plants is provided, including the following steps:

[0014] Soaking plant seeds or irrigating plant seedlings with an aqueous solution of the above composition.

[0015] Preferably, in the aqueous solution of the composition, the concentration of the composition is 50 - 100 mg / L.

[0016] Preferably, the soaking time of plant seeds is 3 - 4 d.

[0017] Preferably, when irrigating plant seedlings, irrigate once every 1 - 2 days, and the irrigation amount for each plant seedling during each irrigation is 100 - 200 mL, and the total irrigation time is 10 - 20 days.

[0018] Advantages of the present invention:

[0019] The present invention provides an application of a composition of gallic acid and p-coumaric acid in promoting plant growth and improving plant salt tolerance. Gallic acid and p-coumaric acid are two naturally occurring phenolic compounds, which are widely distributed in plant secondary metabolites. Application under salt stress conditions can reduce salt damage, increase the length of young leaves and roots of plants, and achieve the purpose of protecting and strengthening seedlings and improving the salt stress resistance of plants in production. Description of the Drawings

[0020] Figure 1 : Growth situation diagram of Nipponbare rice under salt stress with different treatments;

[0021] Figure 2 : Growth situation diagram of Tetep rice under salt stress with different treatments;

[0022] Figure 3:Germination situation diagram of Jimai 22 on day 3 under salt stress with different treatments;

[0023] Figure 4 :Growth situation diagram of Jimai 22 under salt stress with different treatments. Detailed implementation manners

[0024] It should be noted that the following detailed descriptions are all illustrative and are intended to provide further explanations for this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs.

[0025] In order to enable those skilled in the art to more clearly understand the technical solution of this application, the technical solution of this application will be described in detail below in combination with specific embodiments.

[0026] The test materials used in the embodiments of the present invention are all conventional test materials in the art and can be obtained through commercial channels.

[0027] The gallic acid used in the present invention was purchased from Beijing Jinming Biotechnology Co., Ltd.

[0028] The p-coumaric acid used in the present invention was purchased from Tianjin Kaitong Chemical Reagent Co., Ltd.

[0029] Example 1: Preparation of a composition for improving plant salt tolerance:

[0030] Mix gallic acid and p-coumaric acid evenly according to a mass ratio of 1:4 to obtain a composition for improving plant salt tolerance.

[0031] Example 2: Preparation of a composition for improving plant salt tolerance:

[0032] Mix gallic acid and p-coumaric acid evenly according to a mass ratio of 1:2 to obtain a composition for improving plant salt tolerance.

[0033] Example 3: Preparation of a composition for improving plant salt tolerance:

[0034] Mix gallic acid and p-coumaric acid evenly according to a mass ratio of 1:1 to obtain a composition for improving plant salt tolerance.

[0035] Test Example 1: Effect of the composition for improving plant salt tolerance on rice seedlings under salt stress:

[0036] 1. Test rice seeds: Nipponbare rice seeds

[0037] 2. Test method:

[0038] Disinfection treatment of test seeds: Select plump Nipponbare rice seeds, soak them in 0.1% sodium hypochlorite solution for 10 minutes, rinse them once with distilled water, then soak them in distilled water for ten minutes, and rinse. Repeat twice.

[0039] Lay the disinfected Nipponbare rice seeds on a 12-cm glass petri dish pre-laid with sterilized filter paper, and conduct dark cultivation at 25°C for 4 days to germinate. Select 50 germinated seeds with similar growth states.

[0040] A total of 5 treatments were set in the experiment, with 10 seeds in each treatment.

[0041] Treatment group 1: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of a mixed solution containing 125 mmol / L of NaCl and 100 mg / L of the composition for improving plant salt tolerance prepared in Example 1;

[0042] Treatment group 2: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of a mixed solution containing 125 mmol / L of NaCl and 100 mg / L of p-coumaric acid;

[0043] Treatment group 3: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of a mixed solution containing 125 mmol / L of NaCl and 100 mg / L of gallic acid;

[0044] Salt stress treatment group: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of an aqueous solution of NaCl with a concentration of 125 mmol / L;

[0045] Blank control group: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of pure water.

[0046] Cultivate the grouped seeds further using nutrient soil, set the temperature at 28 degrees Celsius, the light at 16 h, and the humidity at 55%. Statistically analyze the young leaf length and root length of the rice seedlings in each treatment group after 14 days. When measuring the young leaf length, take the length of the longest young leaf of the seedling, and the measurement result is the average value of 10 seeds; when measuring the root length, take the length of the longest root of the seedling, and the measurement result is the average value of 10 seeds.

[0047] 3. Test results:

[0048] Table 1 Effects of different treatments on the young leaf length and root length of salt-stressed Nipponbare rice seedlings

[0049] Treatment group Average length of young leaves (cm) Average root length (cm) Treatment group 1 16.3 6.5 Treatment group 2 8.8 5.2 Treatment group 3 15 5.3 Salt stress treatment 7.9 4.3 Blank control group 21 9.5

[0050] From Table 1 and Figure 1It can be seen that, compared with the control, exogenous application of gallic acid and p-coumaric acid under salt stress conditions can significantly promote the growth of plant roots.

[0051] Experimental Example 2: Effect of the composition for improving plant salt tolerance on rice seedlings under salt stress:

[0052] 1. Test rice seeds: Tetep rice seeds

[0053] Disinfection treatment of test seeds: Select plump Tetep rice seeds, soak them in 0.1% sodium hypochlorite solution for 10 minutes, rinse once with distilled water, then soak in distilled water for ten minutes, and rinse, repeating twice.

[0054] Lay the disinfected Tetep rice seeds on a 12-cm glass petri dish pre-laid with sterilized filter paper, and conduct dark cultivation at 25 °C for 4 days of germination. Select 50 germinated seeds with similar growth states.

[0055] A total of 5 treatments were set in the experiment, with 10 seeds in each treatment.

[0056] Treatment group 1: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of a mixed solution containing 100 mmol / L of NaCl and 100 mg / L of the composition for improving plant salt tolerance prepared in Example 1;

[0057] Treatment group 2: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of a mixed solution containing 100 mmol / L of NaCl and 100 mg / L of p-coumaric acid;

[0058] Treatment group 3: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of a mixed solution containing 100 mmol / L of NaCl and 100 mg / L of gallic acid;

[0059] Salt stress treatment group: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of an aqueous solution of NaCl with a concentration of 100 mmol / L;

[0060] Blank control group: On the test day and every two days, water the germinated seeds, and each watering is 200 mL of pure water.

[0061] Cultivate the grouped seeds further using nutrient soil, set the temperature at 28 degrees Celsius, the light at 16 h, and the humidity at 55%. Statistically analyze the young leaf length and root length of the rice seedlings in each treatment group after 14 days. When measuring the young leaf length, take the length of the longest young leaf of the seedling, and the measurement result is the average value of 10 seeds; when measuring the root length, take the length of the longest root of the seedling, and the measurement result is the average value of 10 seeds.

[0062] 3. Test results:

[0063] Table 2 Effects of different treatments on the length of young leaves and roots of Tetep rice seedlings under salt stress

[0064] Treatment group Average length of young leaves (cm) Average root length (cm) Treatment group 1 21 9.5 Treatment group 2 16 6.75 Treatment group 3 14.5 5.2 Salt stress treatment 12.5 6.5 Blank control group 25 12.2

[0065] As can be seen from Table 2 and Figure 2 it is known that compared with the control, exogenous application of gallic acid and p - coumaric acid under salt stress conditions can significantly promote the growth of plant roots.

[0066] Test Example 3: Effects of the composition for improving plant salt tolerance on the germination rate of wheat seeds under salt stress:

[0067] 1. Tested wheat seeds: Jimai 22

[0068] 2. Test method:

[0069] Disinfection treatment of the tested seeds: Select plump wheat seeds, soak them in 0.1% sodium hypochlorite solution for 10 minutes, and rinse them once with distilled water. Then soak them in distilled water for ten minutes, rinse, and repeat twice.

[0070] Take the disinfected wheat seeds, divide them into 5 treatment groups with 60 seeds in each group, which are respectively:

[0071] Treatment group 1: Soak the seeds in a mixed solution containing 150 mmol / L of NaCl and 100 mg / L of the composition for improving plant salt tolerance prepared in Example 2 for 3 days;

[0072] Treatment group 2: Soak the seeds in a mixed solution containing 150 mmol / L of NaCl and 100 mg / L of p - coumaric acid for 3 days;

[0073] Treatment group 3: Soak the seeds in a mixed solution containing 150 mmol / L of NaCl and 100 mg / L of gallic acid for 3 days;

[0074] Salt stress treatment group: Soak the seeds in an aqueous solution of 150 mmol / L of NaCl for 3 days;

[0075] Blank control group: Soak the seeds in pure water for 3 days.

[0076] Carry out dark cultivation at 15°C and observe the germination rate after 3 days.

[0077] Table 3 Effects of different treatments on the germination rate of wheat seeds under salt stress

[0078]

[0079]

[0080] From Figures 3 - 4 Table 3, it can be seen that exogenous application of gallic acid and p-coumaric acid can significantly promote the germination rate of wheat under salt stress conditions compared with the control.

[0081] The above are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition for improving the salt tolerance of plants, characterized in that, The composition consists of gallic acid and p-coumaric acid in a mass ratio of 1:(1 - 4).

2. Use of the composition according to claim 1 in at least one of the following (1)-(2): (1) Improving the salt tolerance of plants; (2) Preparing a product for improving the salt tolerance of plants.

3. The application according to claim 2, characterized in that, The improvement of the salt tolerance of plants means improving the germination rate of seeds, the length of young leaves of seedlings and the root length of seedlings under salt stress.

4. The application according to claim 2, characterized in that The plant is wheat or rice.

5. A method for improving the salt tolerance of plants, characterized in that, It includes the following steps: Soaking plant seeds or irrigating plant seedlings with an aqueous solution of the composition according to claim 1.

6. The method according to claim 5, characterized in that In the aqueous solution of the composition, the concentration of the composition is 50 - 100 mg / L.

7. The method according to claim 5, characterized in that, The soaking time of plant seeds is 3 - 4 days.

8. The method according to claim 5, characterized in that, When irrigating plant seedlings, irrigate once every 1 - 2 days, and the irrigation amount for each plant seedling during each irrigation is 100 - 200 mL, and the total irrigation time is 10 - 20 days.

Images