Composition containing iso-aromatic oxalic acid and taurine and application of composition in relieving salt stress of crops

Abstract

The invention discloses a composition containing iso-aromatic oxalic acid and taurine and application of the composition in relieving salt stress of crops. The composition is composed of iso-aromatic oxalic acid and taurine according to a mass ratio of 1: (1-4). According to the composition containing the iso-aromatic oxalic acid and the taurine, the iso-aromatic oxalic acid and the taurine are compounded according to the specific mass ratio of 1: (1-4), the growth and salt tolerance of crops can be enhanced through the synergistic effect of the iso-aromatic oxalic acid and the taurine, and especially for common food crops such as wheat and rice, the growth and salt tolerance of the crops can be improved. A good growth state can still be kept in a saline-alkali environment, the germination rate of crops is increased, tender leaves and roots are long, and the application prospect in agricultural production is very wide.

Description

Technical Field

[0001] The invention relates to the technical field of crop cultivation, and in particular to a composition containing isovanillic acid and taurine and application of the composition in alleviating salt stress of crops. Background Art

[0002] With the intensification of global climate change and the limited land resources, the area of ​​salinized soil has increased year by year, becoming a major challenge for agricultural production. Salt stress not only affects the absorption of water, but also causes damage to plant cells through increased osmotic pressure, thereby limiting the growth, development and yield of crops. Especially in arid areas and saline-alkali land, traditional agricultural production methods and crop varieties are facing tremendous production pressure. Therefore, the development of new technologies and new materials that can effectively improve the salt resistance of crops has important scientific significance and practical application value.

[0003] Existing technologies for improving crop salt tolerance mainly include bioengineering, soil improvement, the use of chemical regulators, and the use of plant growth regulators. Although bioengineering can cultivate some crop varieties with strong salt tolerance, its cost is high, the operation is complicated, and it is difficult to promote and apply in some areas. Traditional methods such as soil improvement and fertilization usually take effect slowly and have limited effects. As a convenient, economical and quick-acting method, chemical regulators have been increasingly studied and applied in recent years. Plant growth regulators, especially natural compounds, have become an important way to improve crop salt tolerance. Studies have shown that some natural compounds play a significant role in improving plant stress resistance. For example, plant growth hormone substances: such as gibberellins, cytokinins and auxins, these substances have been shown to regulate plant growth and development and improve their salt resistance. Plant phenolic compounds: such as chlorogenic acid, caffeic acid, etc. Plant phenolic compounds have been found to have strong antioxidant properties, which can reduce oxidative damage caused by salt stress and enhance plant stress resistance.

[0004] Although studies have shown that a variety of natural compounds have a positive effect on plant stress resistance, most of these studies focus on the use of a single substance, and there is a lack of research on the synergistic effects between different natural compounds. So far, there has been no report on the combined use of isovanillic acid and taurine to alleviate the effects of salt stress on crops. Summary of the invention

[0005] In view of the above prior art, the object of the present invention is to provide a composition containing isovanillic acid and taurine and application thereof in alleviating salt stress of crops.

[0006] To achieve the above object, the present invention adopts the following technical solution:

[0007] In the first aspect of the present invention, there is provided a composition containing isovanillic acid and taurine, and the composition is composed of isovanillic acid and taurine in a mass ratio of 1:(1 - 4).

[0008] In the second aspect of the present invention, there is provided the use of the above composition in at least one of the following (1)-(2):

[0009] (1) Alleviating salt stress in crops;

[0010] (2) Preparing a product for alleviating salt stress in crops.

[0011] Preferably, the crop is wheat or rice.

[0012] In the third aspect of the present invention, there is provided a method for alleviating salt stress in crops, comprising the following steps:

[0013] Soaking crop seeds or irrigating crop seedlings with an aqueous solution of the above composition.

[0014] Preferably, in the aqueous solution of the composition, the concentration of the composition is 50 - 150 mg / L.

[0015] Preferably, the soaking time of plant seeds is 3 - 4 days.

[0016] Preferably, when irrigating plant seedlings, irrigate once every 1 - 2 days, and the irrigation amount for each plant seedling during each irrigation is 100 - 200 mL, and the total irrigation time is 10 - 20 days.

[0017] Advantages of the present invention:

[0018] The present invention provides a composition containing isovanillic acid and taurine. By compounding isovanillic acid and taurine in a specific mass ratio (1:(1 - 4)), the synergistic effect of isovanillic acid and taurine can enhance the growth and salt tolerance of crops. Especially for common food crops such as wheat and rice, they can still maintain a good growth state in a saline-alkali environment, improve the germination rate, length of young leaves and root length of crops, and have a very broad application prospect in agricultural production. Description of the Drawings

[0019] Figure 1 : Growth situation diagram of Jimai 22 under salt stress with different treatments;

[0020] Figure 2 : Germination situation diagram of Jimai 22 on day 3 under salt stress with different treatments;

[0021] Figure 3 : Growth situation diagram of Tetep under salt stress with different treatments. Detailed Embodiments

[0022] It should be noted that the following detailed description is illustrative and aims to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present application belongs.

[0023] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in conjunction with specific embodiments.

[0024] The test materials used in the embodiments of the present invention are all conventional test materials in the art and can be obtained through commercial channels.

[0025] Isovanillic acid used in the present invention was purchased from Shanghai Macklin Biochemical Co., Ltd.

[0026] Taurine used in the present invention was purchased from Shanghai Macklin Biochemical Co., Ltd.

[0027] Example 1: Preparation of a composition containing isovanillic acid and taurine:

[0028] Mix isovanillic acid and taurine evenly according to a mass ratio of 1:1 to obtain a composition for improving the salt tolerance of plants.

[0029] Example 2: Preparation of a composition containing isovanillic acid and taurine:

[0030] Mix isovanillic acid and taurine evenly according to a mass ratio of 1:2 to obtain a composition for improving the salt tolerance of plants.

[0031] Example 3: Preparation of a composition containing isovanillic acid and taurine:

[0032] Mix isovanillic acid and taurine evenly according to a mass ratio of 1:4 to obtain a composition for improving the salt tolerance of plants.

[0033] Test Example 1: Effect of the composition containing isovanillic acid and taurine on wheat under salt stress:

[0034] 1. Tested wheat seeds: Jimai 22

[0035] 2. Test method:

[0036] Disinfection treatment of tested seeds: Select plump wheat seeds, soak them in 0.1% sodium hypochlorite solution for 10 minutes, and rinse them once with distilled water. Then soak them in distilled water for ten minutes, rinse, and repeat twice.

[0037] Take the disinfected wheat seeds and divide them into 5 treatment groups with 60 seeds each, which are:

[0038] Treatment group 1: Soak the seeds in a mixed solution containing 150 mmol / L of NaCl and 100 mg / L of the composition containing isovanillic acid and taurine prepared in Example 1 for 3 days;

[0039] Treatment group 2: Soak the seeds in a mixed solution containing 150 mmol / L of NaCl and 100 mg / L of isovanillic acid for 3 days;

[0040] Treatment group 3: Soak the seeds in a mixed solution containing 150 mmol / L of NaCl and 100 mg / L of taurine for 3 days;

[0041] Salt stress treatment group: Soak the seeds in an aqueous solution of 150 mmol / L of NaCl for 3 days;

[0042] Blank control group: Soak the seeds in pure water for 3 days.

[0043] Carry out dark culture at 15°C, and count the lengths of the young leaves and young roots of Jimai 22 and the germination rate after 3 days.

[0044] When measuring the length of the young leaves, take the length of the longest young leaf, and the measurement result is the average value of the young leaf lengths of 15 randomly selected germinated seeds; when measuring the length of the young roots, take the length of the longest young root of the seedling, and the measurement result is the average value of the young root lengths of all germinated seeds.

[0045] Table 1 Effects of different treatments on the young leaves and young roots of salt-stressed Jimai 22

[0046] Treatment group Length of young leaf (cm) Length of young root (cm) Treatment group 1 2.11 3.5 Treatment group 2 1.2 2.6 Treatment group 3 1.8 2.6 Salt stress treatment 1.1 1.7 Blank control group 2.4 3.6

[0047] Table 2 Effects of different treatments on the germination rate of salt-stressed wheat seeds

[0048] Germination rate (%) Day1 Day2 Day3 Treatment group 1 0 73.3 (44 grains / 60 grains) 86.7 (52 grains / 60 grains) Treatment group 2 0 65.0 (39 grains / 60 grains) 73.3 (44 grains / 60 grains) Treatment group 3 0 60.0 (36 grains / 60 grains) 71.7 (43 grains / 60 grains) Salt stress treatment 0 53.3 (32 grains / 60 grains) 63.3 (38 grains / 60 grains) Blank control group 0 90.0 (54 grains / 60 grains) 96.7 (58 grains / 60 grains)

[0049] From Figure 1 , Table 1 and Table 2, it can be seen that compared with the control, exogenous application of the composition containing isovanillic acid and taurine under salt stress conditions can significantly promote the germination rate, young leaf length and young root length of wheat, and improve the germination rate of wheat.

[0050] Test example 2: Effects of the composition containing isovanillic acid and taurine on rice seedlings under salt stress:

[0051] 1. Test rice seeds: Tetep

[0052] Disinfection treatment of test seeds: Select plump Tetep seeds, soak them in a 0.1% sodium hypochlorite solution for 10 minutes, rinse once with distilled water, then soak in distilled water for ten minutes, rinse, and repeat twice.

[0053] Lay the disinfected Tetep seeds on a 12-cm glass petri dish with pre-placed sterilized filter paper, and conduct dark cultivation at 25°C for 4 days of germination. Screen 60 germinated seeds with similar growth states.

[0054] A total of 6 treatments were set in the experiment, with 10 seeds in each treatment.

[0055] Compound solution 1:1 treatment group: On the test day and every two days thereafter, water the germinated seeds, each time watering 200 mL of a mixed solution containing 100 mmol / L of NaCl and 100 mg / L of the composition containing isovanillic acid and taurine prepared in Example 1;

[0056] Treatment group 2: On the test day and every two days thereafter, water the germinated seeds, each time watering 200 mL of a mixed solution containing 100 mmol / L of NaCl and 100 mg / L of isovanillic acid;

[0057] Treatment group 3: On the test day and every two days thereafter, water the germinated seeds, each time watering 200 mL of a mixed solution containing 100 mmol / L of NaCl and 100 mg / L of taurine;

[0058] Salt stress treatment group: On the test day and every two days thereafter, water the germinated seeds, each time watering 200 mL of an aqueous solution of 100 mmol / L of NaCl;

[0059] Blank control group: On the test day and every two days thereafter, water the germinated seeds, each time watering 200 mL of pure water.

[0060] Cultivate the grouped seeds further using nutrient soil, set the temperature at 28 degrees Celsius, the light at 16 h, and the humidity at 55%. Statistically analyze the length of the young leaves and the root length of the rice seedlings in each treatment group after 14 days. When measuring the length of the young leaves, take the length of the longest young leaf of the seedling, and the measurement result is the average value of 10 seeds; when measuring the root length, take the length of the longest root of the seedling, and the measurement result is the average value of 10 seeds.

[0061] 3. Experimental results:

[0062] Table 4 Effects of different treatments on the young leaf length and root length of salt-stressed Tetep seedlings

[0063] Treatment group Average length of young leaf (cm) Average root length (cm) Treatment group 1 28.8 9.8 Treatment group 2 25 8 Treatment group 3 24.3 7 Salt stress treatment 11 5.9 Blank control group 28.3 10.5

[0064] As can be seen from Table 4 and Figure 3 It can be known that compared with the control, the compound solution of isovanillic acid:taurine 1:1 has the best alleviating effect on Tetep.

[0065] The above are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.

Claims

1. A composition containing isovanillic acid and taurine, characterized in that, The composition is composed of isovanillic acid and taurine in a mass ratio of 1:(1 - 4).

2. Use of the composition containing isovanillic acid and taurine according to claim 1 in at least one of the following (1)-(2): (1) Alleviating salt stress in crops; (2) Preparing a product for alleviating salt stress in crops.

3. The application according to claim 2, wherein, The crop is wheat or rice.

4. A method for alleviating salt stress in crops, characterized in that, Comprising the following steps: Soaking crop seeds or irrigating crop seedlings with an aqueous solution of the composition according to claim 1.

5. The method according to claim 4, characterized in that, In the aqueous solution of the composition, the concentration of the composition is 50 - 150 mg / L.

6. The method according to claim 4, characterized in that The soaking time of plant seeds is 3 - 4 days.

7. The method according to claim 4, characterized in that When irrigating plant seedlings, irrigate once every 1 - 2 days, and the irrigation amount for each plant seedling during each irrigation is 100 - 200 mL, and the total irrigation time is 10 - 20 days.

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