Insect luring composition

Abstract

The invention belongs to the technical field of disease control, and relates to an insect luring composition. The invention provides an insect luring composition, the insect luring composition comprises a Chinese prickly ash exosome, a Chinese prickly ash extract, a fermentation broth of fragrant fungus L6, Roman chamomile essential oil, jasmine flower oil, alpha-pinnin and laurel, and the strain preservation number of the fragrant fungus L6 is CGMCC No.13634. The insect luring composition has the advantages that the insect luring composition can be used for trapping insects; the insect attracting composition is carefully screened and optimally combined to form an attractant with a remarkable attracting effect on pepper flatheaded borer.

Description

Technical Field

[0001] The present invention belongs to the technical field of pest control and relates to an insect attracting composition. Background Art

[0002] In the fields of agriculture and forestry, insect control has always been an important topic. Traditional insect control methods mainly include the use of chemical pesticides, the setting of physical traps, and biological control, etc. However, these methods have their own advantages and disadvantages. Although chemical pesticides have significant effects, long-term use will cause environmental pollution, damage the ecological balance, and even lead to the development of pesticide resistance in pests. Physical traps are environmentally friendly, but they have high production costs, and the trapping effect is affected by various factors such as weather and terrain. Biological control depends on natural factors such as natural enemies and parasites, and its effect is often difficult to predict and control.

[0003] In recent years, with the in-depth study of insect ethology, methods of attracting and controlling insects by using the tropism of insects (such as phototaxis, chemotaxis, etc.) have gradually attracted attention. Among them, the method of attracting insects by using the chemotaxis of insects to specific chemical substances is particularly remarkable. This method synthesizes or extracts chemical substances with attracting effects artificially to prepare attractants, which attract pests to come into contact, and then achieve the purpose of control.

[0004] Although the method of attracting and controlling insects by using insect chemotaxis has advantages such as environmental protection and high efficiency, there are still some technical problems in actual application. First of all, different species of insects have different chemotaxis to chemical substances, so it is necessary to select appropriate attractant components according to the species of target pests. Secondly, the formula and preparation process of the attractant have an important impact on its attracting effect. An unreasonable formula or preparation process may lead to poor attracting effect or high cost.

[0005] Therefore, in the technical field of pest control, it is particularly important to provide an insect attracting composition or attractant with a reasonable formula. Summary of the Invention

[0006] The present invention provides an insect attracting composition with a reasonable formula. Through careful screening and optimization combination, the composition forms an attractant with a significant attracting effect on Agrilus zanthoxylumi Hou.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] In the first aspect, the present invention provides an insect attracting composition, which includes zanthoxylum exosomes, zanthoxylum extracts, the fermentation broth of Flavobacterium odoratum L6, roman chamomile essential oil, jasmine oil, α-pinene and myrcene. The preservation number of the strain of Flavobacterium odoratum L6 is CGMCC No. 13634.

[0009] Preferably, by weight parts, the insect attracting composition comprises 15 - 20 parts of Zanthoxylum bungeanum exosomes, 25 - 40 parts of Zanthoxylum bungeanum extract, 50 - 70 parts of the fermentation broth of flavoring fungus L6, 2 - 6 parts of Roman chamomile essential oil, 3 - 8 parts of jasmine oil, 8 - 15 parts of α-pinene and 6 - 16 parts of laurelene.

[0010] Preferably, the weight ratio of the Zanthoxylum bungeanum exosomes, the Zanthoxylum bungeanum extract to the fermentation broth of flavoring fungus L6 is 1:2:4.

[0011] Preferably, the weight ratio of the Roman chamomile essential oil to the jasmine oil is 1:2.

[0012] Preferably, the weight ratio of the α-pinene to the laurelene is 1:1.

[0013] Preferably, by weight parts, the insect attracting composition comprises 16 parts of Zanthoxylum bungeanum exosomes, 32 parts of Zanthoxylum bungeanum extract, 64 parts of the fermentation broth of flavoring fungus L6, 4 parts of Roman chamomile essential oil, 8 parts of jasmine oil, 8 parts of α-pinene and 8 parts of laurelene.

[0014] In a second aspect, there is provided a Zanthoxylum buprestis attracting composition, comprising the insect attracting composition of the present invention.

[0015] Then, there is provided a Zanthoxylum buprestis attractant, comprising the Zanthoxylum buprestis attracting composition of the present invention.

[0016] Furthermore, there is provided the application of the Zanthoxylum buprestis attractant of the present invention in the control of Zanthoxylum buprestis.

[0017] In addition, there is provided the application of the insect attracting composition of the present invention in insect control.

[0018] Moreover, there is provided a preparation method of the insect attracting composition of the present invention, and the preparation method is as follows:

[0019] Mix the Zanthoxylum bungeanum exosomes, the Zanthoxylum bungeanum extract, the fermentation broth of flavoring fungus L6, the Roman chamomile essential oil, the jasmine oil, the α-pinene and the laurelene, and then ferment at 30 - 50°C; homogenize the fermented raw materials.

[0020] It should be noted that homogenizing the fermented raw materials can ensure the uniform distribution of each component, thereby improving the stability and effect of the insect attracting composition.

[0021] The flavor bacterium is a microorganism belonging to the genus Myroides. The colony is of medium size, orange-yellow, transparent, with a moist surface and a relatively flat edge. CN106939292A discloses a camel milk lactic acid beverage for clearing the lungs and removing haze, its preparation method and application, which involves the flavor bacterium L6 with the preservation number of CGMCC No.13634. This patent discloses the mixed fermentation of two strains, namely the flavor bacterium L6 and the lactococcus L122, adding a variety of medicinal and edible homologous plants, and adopting preparation processes such as heat preservation extraction and juice crushing. The prepared camel milk lactic acid beverage is light brown with a white color, has a strong milk fragrance, medium acidity and saltiness, and has a faint traditional Chinese medicine flavor. It has been verified by animal experiments that it can resist PM2.5-induced lung inflammation and maintain the inflammatory factors in the lungs without deviating from the normal level.

[0022] Zanthoxylum exosomes are nanoscale vesicles in Zanthoxylum cells. Zanthoxylum exosomes contain 90% of the active ingredients in Zanthoxylum and are the essence of the concentrated active ingredients of Zanthoxylum. Their size is about 30 - 150 nm. These vesicles contain abundant bioactive molecules such as proteins, nucleic acids, lipids, etc., and can participate in processes such as intercellular communication, material exchange, and signal transduction.

[0023] There are significant differences between Zanthoxylum exosomes and Zanthoxylum extracts in terms of source, composition, and function. In terms of source, Zanthoxylum exosomes are derived from nanoscale vesicles released by Zanthoxylum cells under specific physiological or pathological conditions. These vesicles are secreted by cells through the endosome-exocytosis pathway and have a bilayer membrane structure. Zanthoxylum extracts are substances obtained by physical or chemical methods from Zanthoxylum. In terms of composition, Clematis exosomes mainly contain bioactive molecules such as proteins, nucleic acids, lipids, etc. The composition of Clematis extracts is different from that of Clematis exosomes. In terms of function, Zanthoxylum exosomes participate in processes such as intercellular communication, material exchange, and signal transduction. Zanthoxylum extracts are mainly used in fields such as food, solid beverages, and beverages.

[0024] The Zanthoxylum buprestoides belongs to the Coleoptera and the family Buprestidae; it is distributed in the main Zanthoxylum-producing areas across the country. The areas such as Longnan in Gansu, northern Sichuan in Sichuan, and Hancheng in Shaanxi are the most severely affected; it specifically harms Zanthoxylum. The larvae bore into the bark of the branches and trunks, forming irregular boreholes in the cambium, resulting in the outflow of white or brown gummy juice; generally, it bores into the middle and lower parts of the tree trunks, and the area within 30 mm of the base of the main trunk is the most severely affected; the incidence rate of Zanthoxylum trees under 4 - 5 years old is over 70%.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] The insect-attracting composition of the present invention comprises Zanthoxylum bungeanum exosomes, Zanthoxylum bungeanum extract, the fermentation broth of the flavor-producing bacterium L6, Roman chamomile essential oil, jasmine oil, α-pinene, and myrcene. These components are combined through specific ratios and preparation processes to form an attractant that has a significant attracting effect on Agrilus zanthoxylumi. The present invention speculates that: on the one hand, the Zanthoxylum bungeanum exosomes may contain active ingredients that have an attracting effect on Agrilus zanthoxylumi. The flavor-producing bacterium L6 may produce metabolites with special odors, and these metabolites have a natural attracting force on Agrilus zanthoxylumi. The active ingredients in the fermentation broth may mimic the chemical substances in the natural food sources or habitat environments of insects, thereby attracting Agrilus zanthoxylumi. There may be a certain synergistic effect between the Zanthoxylum bungeanum exosomes, the Zanthoxylum bungeanum extract, and the fermentation broth of the flavor-producing bacterium L6, such that they can produce a stronger attracting effect after combination. This synergistic effect may also directly exert a toxic or inhibitory effect on Agrilus zanthoxylumi. On the other hand, the aroma components of the two essential oils, Roman chamomile essential oil and jasmine oil, may combine with the olfactory receptors of Agrilus zanthoxylumi, triggering the foraging behavior of the insects. In addition, these two essential oils may also enhance the overall attractiveness of the composition, making it easier for Agrilus zanthoxylumi to be attracted. On yet another hand, α-pinene and myrcene may mimic certain chemical substances in the ecological environment that have an attracting effect on Agrilus zanthoxylumi.

[0027] (1) By scientifically proportioning a variety of attracting components, the composition of the present invention has a significant attracting effect on Agrilus zanthoxylumi and can effectively attract pests to come into contact.

[0028] (2) The composition of the present invention uses components such as natural plant extracts and microbial fermentation broths, does not contain harmful chemical substances, is environmentally friendly, and is harmless to humans.

[0029] (3) Compared with traditional methods such as chemical pesticides and physical traps, the composition of the present invention has a low preparation cost and is easy to promote and apply.

[0030] (4) The composition of the present invention is not affected by environmental factors such as weather and terrain and can maintain a stable attracting effect under various conditions. Description of the Drawings

[0031] Figure 1 The insect-attracting effect of the insect-attracting composition on Agrilus zanthoxylumi. Detailed Embodiments

[0032] Next, the technical solutions of the present invention will be described in conjunction with the embodiments. However, the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are all conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0033] The flavor-producing bacterium L6 was obtained through a gift, and its deposit number is CGMCC No. 13634. For the record of the flavor-producing bacterium L6, refer to Patent CN106939292A.

[0034] The preparation method of the fermentation broth of the flavor-producing bacterium L6 is as follows:

[0035] Strain activation: Inoculate the flavor-producing bacterium L6 onto a basic solid medium (20 g of glucose, 10 g of peptone, 5 g of yeast extract powder, 5 g of NaCl, 1 g of K2HPO4, 0.5 g of MgSO4·7H2O, 15 g of agar, 1000 L of H2O) for cultivation to activate the strain.

[0036] Seed liquid preparation: Transfer the activated flavor-producing bacterium L6 into a basic liquid medium (20 g of glucose, 10 g of peptone, 5 g of yeast extract powder, 5 g of NaCl, 1 g of K2HPO4, 0.5 g of MgSO4·7H2O, 1000 L of H2O); use a 500 mL Erlenmeyer flask, and put 100 mL of TSA liquid medium in each flask. Incubate at 30 °C with a shaking speed of 180 rpm for 18 h to obtain the seed liquid.

[0037] Fermentation culture: Inoculate the seed liquid into the fermentation medium at a ratio of 1:100. Incubate at 30 °C with a shaking speed of 200 rpm for 48 h. After the cultivation is completed, the fermentation broth of the flavor-producing bacterium L6 can be obtained.

[0038] Components of the fermentation medium (g / L): 15 of glucose, 6 of peptone, 5 of yeast extract powder, 5 of K2HPO4, 5 of NaCl, pH 7.0 - 7.3.

[0039] Use and dilution: In actual use, the fermentation broth can be diluted according to needs before use.

[0040] The preparation method of the Zanthoxylum bungeanum exosomes is as follows:

[0041] ① Add 350 mL of sterile PBS to 260 g of fresh and uncontaminated Zanthoxylum bungeanum samples, and use a crusher to break them. After breaking, centrifuge at 5 °C and 3500×g for 35 min to remove larger residues and precipitates, and take the clear solution in the middle layer;

[0042] ② Centrifuge the clear solution obtained in step ① at 5 °C and 6000×g for 40 min, and take the supernatant;

[0043] ③ Centrifuge the supernatant obtained in step ② at 5 °C and 90000×g for 45 min, then resuspend the precipitate with 5 mL of sterile PBS at 5 °C and centrifuge at 110000×g for 50 min. Resuspend the precipitate with 5 mL of sterile PBS again to obtain the Zanthoxylum bungeanum exosome solution.

[0044] The preparation method of the Chinese prickly ash extract is as follows:

[0045] After drying and crushing the Chinese prickly ash, the effective components (such as volatile oil, anthocyanin, etc.) in the Chinese prickly ash are extracted by steam distillation method;

[0046] The obtained liquid is subjected to separation operations such as filtration and centrifugation to obtain a pure Chinese prickly ash extract.

[0047] The preparation method of the insect attracting composition is as follows:

[0048] Mix the Chinese prickly ash exosomes, Chinese prickly ash extract, fermentation broth of flavor bacteria L6, Roman chamomile essential oil, jasmine oil, α-pinene and laurelene, and then ferment at 30 - 50 °C; homogenize the fermented raw materials.

[0049] Example 1

[0050] This example provides an insect attracting composition. By weight, the insect attracting composition includes 15 parts of Chinese prickly ash exosomes, 25 parts of Chinese prickly ash extract, 50 parts of fermentation broth of flavor bacteria L6, 2 parts of Roman chamomile essential oil, 3 parts of jasmine oil, 8 parts of α-pinene and 6 parts of laurelene.

[0051] Example 2

[0052] This example provides an insect attracting composition. By weight, the insect attracting composition includes 20 parts of Chinese prickly ash exosomes, 40 parts of Chinese prickly ash extract, 70 parts of fermentation broth of flavor bacteria L6, 6 parts of Roman chamomile essential oil, 8 parts of jasmine oil, 15 parts of α-pinene and 16 parts of laurelene.

[0053] Example 3

[0054] This example provides an insect attracting composition. By weight, the insect attracting composition includes 16 parts of Chinese prickly ash exosomes, 32 parts of Chinese prickly ash extract, 64 parts of fermentation broth of flavor bacteria L6, 4 parts of Roman chamomile essential oil, 8 parts of jasmine oil, 8 parts of α-pinene and 8 parts of laurelene.

[0055] Comparative Example 1

[0056] Compared with Example 3, the difference of this comparative example is that no Chinese prickly ash exosomes are added.

[0057] Comparative Example 2

[0058] Compared with Example 3, the difference of this comparative example is that no fermentation broth of flavor bacteria L6 is added.

[0059] Comparative Example 3

[0060] Compared with Example 3, the difference in this comparative example is that Roman chamomile essential oil is replaced with Sophora japonica flower essential oil.

[0061] Effect verification

[0062] Experimental subjects: The insect-attracting compositions prepared in Examples 1-3 and Comparative Examples 1-3.

[0063] Experimental method: Select a 200-acre Chinese prickly ash forest in Banqiao Town, Hancheng City, Shaanxi Province with the same variety, growth trend, and management conditions of Chinese prickly ash as the test field; use the insect-attracting compositions prepared in Examples 1-3 and Comparative Examples 1-3 to set up traps, hang the traps containing the insect-attracting compositions in the Chinese prickly ash forest, collect the attracted Agrilus zanthoxylumi under the traps, randomly set 5 points evenly for each test group, collect the number of Agrilus zanthoxylumi trapped every 5 days, and continuously collect for 20 days, and count the number.

[0064] Experimental results: As shown in Table 1, Figure 1 as follows.

[0065] Table 1 Insect-trapping effects of each test group on Agrilus zanthoxylumi

[0066]

[0067] It can be seen from Table 1 that the insect-attracting composition provided by the present invention shows excellent performance in the control of Agrilus zanthoxylumi. This control effect is achieved through the attracting effect. Specifically, the number of Agrilus zanthoxylumi attracted by the insect-attracting composition of the present invention is 381-456, which is significantly higher than that of the insect-attracting composition without using the formula system of the present invention. The number of Agrilus zanthoxylumi killed by the insect-attracting composition of the present invention is 191-229, which is significantly higher than that of the insect-attracting composition without using the formula system of the present invention. In addition, the insect-attracting composition of the present invention does not cause the death of surrounding plants.

[0068] From Figure 1 and Table 1, it can be seen that the number of Agrilus zanthoxylumi attracted by the insect-attracting compositions of the present invention shown in Examples 1-3 is significantly higher than that of the insect-attracting compositions without using the formula system of the present invention shown in Comparative Examples 1-3. The number of Agrilus zanthoxylumi killed by the insect-attracting compositions of the present invention shown in Examples 1-3 is significantly higher than that of the insect-attracting compositions without using the formula system of the present invention shown in Comparative Examples 1-3. Moreover, the insect-attracting composition of the present invention shown in Example 3 has the largest number of Agrilus zanthoxylumi attracted and the largest number of killed, and does not cause harm to surrounding plants.

[0069] It should be understood that the present invention disclosed is not limited to the specific methods, schemes and substances described, as these can vary. It should also be understood that the terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of the present invention, which is limited only by the appended claims.

Claims

1. An insect attracting composition, characterized in that, The insect-attracting composition comprises Zanthoxylum bungeanum exosomes, Zanthoxylum bungeanum extract, the fermentation broth of flavor bacteria L6, Roman chamomile essential oil, jasmine oil, α-pinene and myrcene. The preservation number of the strain of flavor bacteria L6 is CGMCC No. 13634.

2. The insect attracting composition according to claim 1, wherein By weight, the insect-attracting composition comprises 15-20 parts of Zanthoxylum bungeanum exosomes, 25-40 parts of Zanthoxylum bungeanum extract, 50-70 parts of the fermentation broth of flavor bacteria L6, 2-6 parts of Roman chamomile essential oil, 3-8 parts of jasmine oil, 8-15 parts of α-pinene and 6-16 parts of myrcene.

3. The insect attracting composition according to claim 1, characterized in that, The weight ratio of the Zanthoxylum bungeanum exosomes, the Zanthoxylum bungeanum extract to the fermentation broth of flavor bacteria L6 is 1:2:

4.

4. The insect attracting composition according to claim 1, wherein The weight ratio of the Roman chamomile essential oil to the jasmine oil is 1:

2.

5. The insect attracting composition according to claim 1, wherein The weight ratio of the α-pinene to the myrcene is 1:

1.

6. The insect attracting composition according to claim 1, wherein By weight, the insect-attracting composition comprises 16 parts of Zanthoxylum bungeanum exosomes, 32 parts of Zanthoxylum bungeanum extract, 64 parts of the fermentation broth of flavor bacteria L6, 4 parts of Roman chamomile essential oil, 8 parts of jasmine oil, 8 parts of α-pinene and 8 parts of myrcene.

7. A lure composition for Agrilus zanthoxylumi, characterized in that, Comprising the insect-attracting composition according to any one of claims 1-6.

8. A lure for Agrilus zanthoxylumi, characterized in that, Comprising the Zanthoxylum buprestoides-attracting composition according to claim 7.

9. Use of the Zanthoxylum buprestoides attractant according to claim 8 in the control of Zanthoxylum buprestoides.

10. Use of the insect-attracting composition according to any one of claims 1-6 in insect control.

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