Composite highland barley tea with effects of dispelling effects of alcohol and protecting liver and preparation method of composite highland barley tea

Abstract

The invention discloses composite highland barley tea with effects of dispelling effects of alcohol and protecting liver and a preparation method of the composite highland barley tea, and belongs to the technical field of tea bag processing. Comprising 50-70 parts of highland barley, 3-4 parts of radix puerariae, 8-12 parts of ampelopsis grossedentata, 8-12 parts of red Chinese wolfberry fruits, 2-4 parts of chrysanthemum morifolium ramat and 0.2-0.4 part of stevia rebaudiana, after the raw materials are mixed, the mixed tea product is put into a corn fiber tea bag by using a tea bag packaging machine, and the prepared highland barley tea product capable of dispelling the effects of alcohol and protecting the liver retains bioactive components of various raw materials to the maximum extent, and has the effects of dispelling the effects of alcohol and protecting the liver. All the raw materials have a synergistic effect, and the tea is convenient to carry, brewed with boiled water and convenient to drink.

Description

Technical Field

[0001] The present invention relates to the technical field of processing of teabags, and in particular to a compound highland barley tea with the function of relieving hangover and protecting the liver and a preparation method thereof. Background Art

[0002] China is the birthplace of wine culture and is one of the earliest countries in the world to make wine. Moderate drinking can promote blood circulation, dredge the channels and collaterals, and dispel wind and dampness. However, excessive drinking will cause a great burden on the body, leading to symptoms such as nausea, vomiting, inattention, impaired fine motor ability, and emotional instability. Long-term excessive drinking will damage internal organs such as the liver, stomach, and spleen, and may lead to diseases such as mental disorders, gastric ulcers, fatty liver, alcoholic hepatitis, and liver cirrhosis. Therefore, relieving hangover and protecting the liver is the research content of many scholars. Using medicinal and edible Chinese herbal medicines to treat alcoholic liver disease can avoid the harm of drugs to the patient's body, and at the same time can avoid the adverse reactions caused by drugs, with high safety, and has become a new direction for the treatment of alcoholic liver disease and also a new direction for the research and development of current hangover products. China has a profound and long history of tea culture. Tea and wine often go hand in hand, and the tea polyphenols contained in tea have the effect of relieving hangover. Therefore, the research and development of tea beverages is an effective way to develop hangover products.

[0003] Highland barley tea is a traditional plateau tea with the effects of being green and healthy, lowering blood pressure, lowering blood lipids, and reducing weight and fat. Highland barley has rich nutritional value and outstanding medical and health care functions. According to "Supplements to Materia Medica", highland barley can relieve qi, widen the middle, strengthen the essence and strength, dispel dampness and induce sweating, and stop diarrhea. The Tibetan medical classic "Jingzhu Materia Medica" even regards highland barley as an important medicine for treating various diseases. However, highland barley tea has a small number of suitable people, does not meet the taste of modern people, and has a single effect, and cannot meet the physical needs of modern people who are in a sub-healthy state due to long-term staying in the office.

[0004] Nowadays, teabags are favored and concerned by more and more people. They are convenient to carry, have quantitative packaging, short brewing time, simple procedures, and easy tea residue disposal. In recent years, with the increasing living standards of the public and the improvement of people's requirements for health, at present, most teabags on the market have complex ingredients, and due to inaccurate dosages and lack of scientific ratios, the products are uneven, and the efficacy and safety effects on the human body cannot be accurately evaluated.

[0005] In view of the above situation, researching a highland barley tea with the function of relieving hangover and protecting the liver is an effective way to solve the above technical problems. Summary of the Invention

[0006] In view of the above problems existing in the prior art, the present invention provides a compound highland barley tea with the function of relieving hangover and protecting the liver and a preparation method thereof.

[0007] The technical solution of the present invention is as follows:

[0008] The first object of the present invention is to provide a compound highland barley tea with the function of relieving hangover and protecting the liver, which is composed of the following raw materials in parts by weight: 50-70 parts of highland barley, 3-4 parts of kudzu root, 8-12 parts of rattan tea, 8-12 parts of red wolfberry, 2-4 parts of Hangzhou white chrysanthemum, and 0.2-0.4 parts of stevia rebaudiana.

[0009] In one embodiment of the present invention, the highland barley is the cleaned wheat obtained after screening, stone removal, wheat beating, wheat brushing, circulating air separation, and color sorting after entering the warehouse.

[0010] In one embodiment of the present invention, the highland barley is yellow highland barley.

[0011] In one embodiment of the present invention, the highland barley is stir-fried by gradient. The stir-frying method is: mixing the cleaned wheat with 8-10% water, pouring it into a preheated wok and tumbling and stir-frying, then raising the temperature and continuing to stir-fry until the surface of the highland barley turns brownish-yellow, and then quickly pouring it out of the wok and cooling it to room temperature.

[0012] In one embodiment of the present invention, the preheating temperature is 225-235 °C, and the stir-frying time is 40-50 min.

[0013] In one embodiment of the present invention, the temperature is raised to 265-275 °C and stir-fried for 15-20 min.

[0014] In one embodiment of the present invention, the kudzu root, rattan tea, red wolfberry, Hangzhou white chrysanthemum, and stevia leaf are respectively naturally air-dried or dried at 50-70 °C until the moisture content is lower than 10%, and then subjected to coarse crushing treatment with a mesh number of 5-15 meshes for standby.

[0015] The second object of the present invention is to provide a preparation method of the above compound highland barley tea, which includes the following steps:

[0016] Mix 50-70 parts of highland barley, 3-4 parts of kudzu root, 8-12 parts of rattan tea, 8-12 parts of red wolfberry, 2-4 parts of Hangzhou white chrysanthemum, and 0.2-0.4 parts of stevia rebaudiana; use a teabag packaging machine for sub-packaging to obtain the bagged compound highland barley tea.

[0017] In one embodiment of the present invention, the material of the tea bag is corn fiber.

[0018] In one embodiment of the present invention, the specification of the tea bag is 8.2-9 g / bag.

[0019] The present invention provides a highland barley teabag product, which has the health care effect of relieving hangover and protecting the liver when drunk or in daily life.

[0020] The beneficial technical effects of the present invention are as follows:

[0021] The hulless barley, kudzu root, tengcha (Ampelopsis grossedentata), wolfberry, etc. used in the present invention are common natural plant resources, rich in nutritional components and active ingredients, and have various pharmacological activities such as hypoglycemic, hypolipidemic, antioxidant, anti-aging, heat-clearing and detoxifying, and hangover and liver protection. Moreover, the prepared hulless barley hangover and liver protection tea product maximally retains the bioactive components of various raw materials, and various raw materials synergistically enhance the effect. It can be brewed with boiling water, is convenient to drink, also has the effect of hangover and liver protection, and its production process is simple, the preparation cost is low, and it is suitable for drinking during or in daily life, meeting the general requirements of people for natural, safe and healthy food.

[0022] Drinking one bag of the bagged compound hulless barley tea of the present invention per day can achieve the effect of hangover and liver protection. The results of animal experiments show that when mice are fed medium and high doses of hulless barley tea, significant changes occur in indicators such as AST, ALT, ALP, and LDH in the serum of mice, as well as TG, SOD, MDA, GSH, and GSH-PX in the liver tissue. Description of the Drawings

[0023] Figure 1 It is the liquid chromatogram of puerarin and dihydromyricetin.

[0024] Figure 2 It is a schematic diagram of microscopic observation of stained mouse liver tissue sections.

[0025] Figure 3 It is a schematic diagram of the protein expression level in mouse liver tissue.

[0026] Figure 4 It is the Chao1 index of the Alpha diversity of the mouse gut microbiota.

[0027] Figure 5 It is the Ace index of the Alpha diversity of the mouse gut microbiota.

[0028] Figure 6 It is the Shannon index of the Alpha diversity of the mouse gut microbiota.

[0029] Figure 7 It is the Simpson index of the Alpha diversity of the mouse gut microbiota.

[0030] Figure 8 It is the principal component analysis graph of the Beta diversity of the mouse gut microbiota.

[0031] Figure 9 It is the principal coordinate analysis graph of the Beta diversity of the mouse gut microbiota.

[0032] Figure 10 It is the microbiota distribution map at the phylum level of the mouse gut microbiota.

[0033] Figure 11This is the distribution map of the intestinal flora at the genus level in mice. DETAILED DESCRIPTION

[0034] The present invention is described in detail below in conjunction with the accompanying drawings and embodiments.

[0035] Raw materials and reagents in the present invention:

[0036] Raw materials: highland barley, kudzu root, rattan tea, red wolfberry, chrysanthemum, stevia leaf, Qinghai Province Xindingxiang Grain and Oil Co., Ltd. Reagents: dihydromyricetin, puerarin, Shanghai MacLean Biochemical Technology Co., Ltd.; gallic acid, rutin, methanol, sulfuric acid, folinol, Sinopharm Chemical Reagent Group Co., Ltd. Kits: mouse tumor necrosis factor α test kit, mouse interleukin 6 test kit, mouse interleukin 1β test kit, Nanjing Senbeijia Biotechnology Co., Ltd.; alanine aminotransferase test kit, aspartate aminotransferase test kit, alkaline phosphatase test kit, lactate dehydrogenase test kit, glutathione peroxidase test kit, reduced glutathione test kit, superoxide dismutase test kit, malondialdehyde test kit, low-density lipoprotein cholesterol assay kit, high-density lipoprotein cholesterol assay kit, total cholesterol assay kit, triglyceride assay kit, alcohol dehydrogenase assay kit, acetaldehyde dehydrogenase assay kit, BCA kit, Nanjing Jiancheng Bioengineering Institute; MyD88, PPARα, CYP2E1, Nrf2, AMPK and TLR4 antibodies, Wuxi Jianzhou Biotechnology Co., Ltd.

[0037] The apparatus and equipment in the present invention:

[0038] AX224ZH / E electronic analytical balance, Ohaus Instrument (Changzhou) Co., Ltd.; HHS digital display constant temperature water bath, medical equipment of Shanghai Boxun Industrial Co., Ltd.; LXJ-Ⅱ high-speed centrifuge, Shanghai Anting Scientific Instrument Factory; UV-3200 ultraviolet spectrophotometer, Shanghai Mepta Instrument Co., Ltd.; FS-1 electric homogenizer, Shanghai Bilang Instrument Co., Ltd.; Agilent1260 high performance liquid chromatograph, Agilent Technologies, USA; RT-6100 microplate reader, Shenzhen Raydu Life Sciences Co., Ltd.; MS-PB magnetic stirrer, TSY-B decolorization shaker, BV-2 vertical electrophoresis instrument, KZ-II grinder, Wuhan Savier Biotechnology Co., Ltd.

[0039] Example 1

[0040] A method for preparing bagged composite highland barley tea comprises the following steps:

[0041] (1) After the yellow highland barley is stored in the warehouse, it is subjected to screening, stone removal, wheat beating, wheat brushing, cyclic air separation, and color sorting to obtain clean wheat. The clean wheat is mixed with 9% water, poured into a frying pan preheated to 230 °C, and stir-fried for 45 minutes while tumbling. Then the temperature is raised to 270 °C and stir-fried for 20 minutes until the surface of the highland barley turns brownish-yellow. Then it is quickly poured out of the frying pan and cooled to room temperature.

[0042] (2) Kudzu root, rattan tea, red wolfberry, Hangzhou white chrysanthemum, and stevia leaves are naturally dried until the moisture content is lower than 10%, and then coarsely ground and sieved through a 10-mesh sieve.

[0043] (3) Take 60 parts of yellow highland barley, 3.5 parts of kudzu root, 10 parts of rattan tea, 10 parts of red wolfberry, 3 parts of Hangzhou white chrysanthemum, and 0.3 parts of stevia. After mixing the raw materials, use a teabag packaging machine to pack the mixed tea product into a corn fiber tea bag, and you will get the bagged compound highland barley tea (8 - 9 g).

[0044] Example 2

[0045] A preparation method of bagged compound highland barley tea, comprising the following steps:

[0046] (1) After the yellow highland barley is stored in the warehouse, it is subjected to screening, stone removal, wheat beating, wheat brushing, cyclic air separation, and color sorting to obtain clean wheat. The clean wheat is mixed with 10% water, poured into a frying pan preheated to 230 °C, and stir-fried for 50 minutes while tumbling. Then the temperature is raised to 270 °C and stir-fried for 15 minutes until the surface of the highland barley turns brownish-yellow. Then it is quickly poured out of the frying pan and cooled to room temperature.

[0047] (2) Kudzu root, rattan tea, red wolfberry, Hangzhou white chrysanthemum, and stevia leaves are naturally dried until the moisture content is lower than 10%, and then coarsely ground and sieved through a 10-mesh sieve.

[0048] (3) Take 50 parts of yellow highland barley, 3 parts of kudzu root, 8 parts of rattan tea, 12 parts of red wolfberry, 4 parts of Hangzhou white chrysanthemum, and 0.2 parts of stevia. After mixing the raw and auxiliary materials, use a teabag packaging machine to pack the mixed tea product into a corn fiber tea bag, and you will get the bagged compound highland barley tea (8 - 9 g).

[0049] Example 3

[0050] A preparation method of bagged compound highland barley tea, comprising the following steps:

[0051] (1) After the yellow highland barley is stored in the warehouse, it is subjected to screening, stone removal, wheat beating, wheat brushing, cyclic air separation, and color sorting to obtain clean wheat. The clean wheat is mixed with 8% water, poured into a frying pan preheated to 225 °C, and stir-fried for 40 minutes while tumbling. Then the temperature is raised to 275 °C and stir-fried for 18 minutes until the surface of the highland barley turns brownish-yellow. Then it is quickly poured out of the frying pan and cooled to room temperature.

[0052] (2) Kudzu root, rattan tea, red wolfberry, Hangzhou white chrysanthemum, and stevia leaves are respectively air-dried naturally until the moisture content is lower than 10%, and then coarsely crushed and sieved through a 10-mesh sieve.

[0053] (3) Take 70 parts of hulless barley, 4 parts of kudzu root, 12 parts of rattan tea, 12 parts of red wolfberry, 4 parts of Hangzhou white chrysanthemum, and 0.4 parts of stevia. After mixing the raw and auxiliary materials, use a teabag packaging machine to pack the mixed tea products into corn fiber teabag bags, and you will get the compound hulless barley teabag (8 - 9 g).

[0054] Comparative Example 1

[0055] (1) After the hulless barley is put into the warehouse, it is subjected to screening, stone removal, wheat beating, wheat brushing, circulating air separation, and color sorting to obtain clean wheat. The clean wheat is mixed with 9% water, poured into a frying pan preheated to 230 °C and stir-fried for 45 min, then the temperature is raised to 270 °C and stir-fried for 20 min until the surface of the hulless barley turns brownish-yellow, and then quickly poured out of the frying pan and cooled to room temperature.

[0056] (2) Take 60 parts of hulless barley, and use a teabag packaging machine to pack the hulless barley tea products into corn fiber teabag bags to obtain the hulless barley teabag.

[0057] Comparative Example 2

[0058] (1) Take 3.5 parts of kudzu root, 10 parts of rattan tea, 10 parts of red wolfberry, 3 parts of Hangzhou white chrysanthemum, and 0.3 parts of stevia. After mixing the auxiliary materials, use a teabag packaging machine to pack the mixed tea products into corn fiber teabag bags to obtain the teabag without hulless barley.

[0059] Test Example:

[0060] 1.1 Sample Preparation

[0061] Take the teabag in Example 1 and put it into 300 mL of boiling water, soak for 15 min, take out the teabag, keep the tea soup, repeat the operation, add 300 mL of boiling water, soak for 15 min, take out the teabag, and fully mix the two tea soups to obtain the total tea soup. Part of it is reserved for use in measuring the active substances in the tea soup; part of it is subjected to reduced-pressure rotary evaporation and freeze-drying treatment to obtain the freeze-dried powder of the tea soup, which is redissolved and fixed in volume for gavage use (fixed volume of 15 ml), and record the gavage sample 1.

[0062] Comparative Example 1 and Comparative Example 2 are operated in the same way as above to obtain the gavage sample 2 and the gavage sample 3.

[0063] 1.2 Determination of Active Substances in Tea Soup

[0064] Use the phenol-sulfuric acid determination method to measure the concentration of total sugar;

[0065] The contents of total flavonoids and total polyphenols are determined by ultraviolet spectrophotometry (Shanghai Meipuda Instrument Co., Ltd.);

[0066] Puerarin and dihydromyricetin were quantified by high performance liquid chromatography (HPLC). Chromatographic separation was performed using an Agilent 1260 high performance liquid chromatograph. Chromatographic column: Kromasil C18 column (150 mm × 4.6 μm); mobile phase: aqueous solution of 0.1% phosphoric acid (A) - methanol (B), flow rate: 1 mL / min; column temperature: 30 °C; detection wavelength: 250 nm for puerarin / 285 nm for dihydromyricetin; injection volume: 10 μL; elution gradient is shown in Table 1.

[0067] Table 1 Liquid chromatography elution gradient

[0068] Time (min) Flow rate (mL / min) Mobile phase A (%) Mobile phase B (%) 0 1 90 10 15 1 40 60 18 1 10 90 20 1 10 90 23 1 90 10 30 1 90 10

[0069] The content of active substances that can be brewed from each packet of compound bagged highland barley tea (Example 1) is shown in Table 2, where the polyphenol content is 143.39 ± 2.72 mg / bag, the flavonoid content is 24.56 ± 0.39 mg / bag, the polysaccharide content is 419.3 ± 4.47 mg / bag, the dihydromyricetin content is 96.08 ± 9.48 mg / bag, and the puerarin content is 4.43 ± 1.24 mg / bag.

[0070] Table 2 Content of active substances in the tea soup

[0071] Content of active substances in tea soup Polyphenols (mg / bag) 143.39±2.72 Flavonoids (mg / bag) 24.56±0.39 Polysaccharides (mg / bag) 419.3±4.47 Dihydromyricetin (mg / bag) 96.08±9.48 Puerarin (mg / bag) 4.43±1.24

[0072] 1.3 Animal experiments

[0073] 1.3.1 Animal experiment method

[0074] After pre-breeding for 7 days, the mice in the blank group continued to be fed with ordinary feed and water, and the mice in the other groups were intragastrically administered alcohol (0.1 mL of 43° highland barley wine per 10 g of mice) at a fixed time every day for 7 days. Starting from the 15th day, alcohol was intragastrically administered once a day at a fixed time and the drug and liver-protecting tea (0.1 mL per 10 g of mice) were intragastrically administered 2 hours later for three consecutive weeks: the blank group was always fed with ordinary feed and water; the negative control group was intragastrically administered alcohol + normal saline; the positive control group was intragastrically administered alcohol + drug; the Example 1 group was intragastrically administered alcohol + intragastrically administered sample 1; the Comparative Example 1 group was intragastrically administered alcohol + intragastrically administered sample 2; the Comparative Example 2 group was intragastrically administered alcohol + intragastrically administered sample 3. The mice were weighed every three days, and the weight changes of the mice were recorded and the doses of the drug and the bagged tea were adjusted. After 3 weeks of breeding, the mice were sacrificed, and blood was collected by orbital venous plexus blood collection method. The serum was centrifuged and used for the detection of indicators such as AST and ALT. After blood collection, the liver was taken out, the blood stain was blotted dry with filter paper and weighed, 1 / 3 of the largest lobe was cut off, fixed in 4% formaldehyde solution, and the remaining liver tissue was stored in a -80 °C refrigerator.

[0075] Experimental procedure: Mice were pre-fed with ordinary feed in the animal house for 7 days. From the 8th day to the 14th day, they were given alcohol by gavage, and then the model was established for 3 weeks. The negative control group was gavaged with normal saline (0.1 mL / 10 g); the positive drug group was gavaged with bifendate pill solution: prepared with bifendate pills. Dissolve bifendate pills in water to prepare an aqueous solution of 15 mg / mL, and the dosage for mice was 0.1 mL / 10 g, that is, 1.5 mg / 10 g. Shake well before use; self-prepare and concentrate the tea bags and gavage at a fixed time every day. After 3 weeks, the mice were sacrificed, 1.5 mL of blood was collected by orbital venous plexus blood sampling method, the liver was taken and weighed, and the liver-body ratio was calculated.

[0076] Purchase kits to measure the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in mouse serum. Use ELISA kits to detect the levels of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and interleukin 1β (IL-1β) in mouse serum; for mouse liver tissue, add 0.86% normal saline according to the ratio of tissue weight (g): normal saline volume (mL) = 1:5 - 1:10, cut the tissue into pieces, prepare a homogenate in an ice-water bath, centrifuge at 2500 - 3000 revolutions per minute for 10 minutes, and take the supernatant to detect high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), superoxide dismutase (SOD), malondialdehyde (MDA), aldehyde dehydrogenase (ALDH), reduced glutathione (GSH) and glutathione peroxidase (GSH-PX). And observe the mental state of the mice every day until the end of the experiment.

[0077] 1.3.2 Determination of serum indexes

[0078] The levels of AST, ALT, ALP, and LDH in serum were detected according to the method provided by the corresponding kit. The results are shown in Table 3. Compared with the blank control group, the levels of AST, ALT, ALP, and LDH in the serum of the negative group mice were significantly increased. The levels of AST, ALT, ALP, and LDH in the serum of the negative group mice increased by 88.38%, 79.65%, 32.44%, and 57.13%, respectively. After the intervention with the drug and the tea bag, except for a slight increase in AST in the serum of the mice in Comparative Example 1, the levels of other indicators decreased, but the changes in Comparative Example 1 and Comparative Example 2 were not significant compared with the model group. Among them, the ALT levels decreased by 18.71% and 14.71%, respectively, the ALP levels decreased by 2.44% and 8.37%, respectively, and the LDH levels decreased by 14.82% and 16.69%, respectively. The drug and Example 1 were the most significant. Compared with the negative group, the AST levels in the serum of the positive drug and Example 1 mice decreased by 35.33% and 35.66%, respectively, the ALT levels decreased by 32.35% and 34.12%, respectively, the ALP levels decreased by 14.31% and 14.93%, respectively, and the LDH levels decreased by 22.09% and 24.26%, respectively. In summary, Example 1 showed excellent performance in terms of the levels of AST, AST, ALT, ALP, and LDH in serum, and the effects of AST, AST, and ALT were greater than the sum of those in Comparative Example 1 and Comparative Example 2, showing an effect of 1 + 1 > 2 and having an obvious synergistic effect.

[0079] Table 3 Results of Serum Index Determination

[0080] Group AST (U / L) ALT (U / L) ALP (U / L) LDH (U / L) Blank 6.47±0.79 4.73±0.81 67.55±2.23 1157.66±89.75 Negative 12.2±2.65 8.5±2.04 89.46±5.9 1819.07±179.77 Positive 7.89±0.93 5.75±0.7 76.66±6.92 1417.27±145.88 Example 1 7.85±0.43 5.6±0.95 76.1±3.21 1377.78±155.45 Comparative Example 1 12.37±0.58 6.91±1.2 87.28±2.94 1549.55±66.72 Comparative Example 2 10.73±2.33 7.25±0.71 81.97±5.46 1515.47±191.85

[0081] 1.3.3 Serum Inflammatory Factor Levels

[0082] The levels of TNF-α, IL-6, and IL-1β in serum were detected according to the method provided by the corresponding kit. The results are shown in Table 4. Compared with the blank control group, the levels of TNF-α, IL-6, and IL-1β in the serum of the negative group mice were significantly increased. The levels of TNF-α, IL-6, and IL-1β in the serum of the negative group mice increased by 72.57%, 43.73%, and 47.08% respectively. After the intervention with the drug and the tea bag, the levels of TNF-α, IL-6, and IL-1β in the serum of each group of mice decreased to varying degrees. Among them, the changes in the levels of TNF-α and IL-6 in the serum of the mice in Comparative Example 1 were not significant, and the level of IL-1β decreased by 7.49%. The change in the level of IL-6 in the serum of the mice in Comparative Example 2 was not significant, the level of TNF-α decreased by 22.3%, and the level of IL-1β decreased by 10.61%. The effect of the drug and Example 1 was the most significant. Compared with the negative group, the levels of TNF-α in the serum of the positive drug and Example 1 mice decreased by 33.40% and 28.55% respectively, the levels of IL-6 decreased by 15.04% and 12.4% respectively, and the levels of IL-1β decreased by 25.64% and 20.24% respectively. To sum up, Example 1 showed excellent performance in terms of the levels of TNF-α, IL-6, and IL-1β in serum, could reduce the levels of serum inflammatory factors in alcoholic liver injury mice, and the effects of TNF-α, IL-6, and IL-1β were greater than the sum of those in Comparative Example 1 and Comparative Example 2, showing an effect of 1 + 1 > 2. There was an obvious synergistic effect between hulless barley and other substances. Table 4 Results of the determination of serum inflammatory factor indicators

[0083] Group TNF-α (ng / L) IL-6 (pg / mL) IL-1β (ng / L) Blank group 303.11±9.27 82.06±1.98 73.73±1.65 Negative group 523.07±37.13 117.95±6.78 108.45±4.17 Positive group 348.37±18.24 100.2±4.42 80.64±3.79 Example 1 373.76±7.71 103.32±2.98 86.5±4.37 Comparative Example 1 486.64±23.7 117.59±5.59 100.33±3.45 Comparative Example 2 406.41±15.9 110.19±5.02 96.94±3.32

[0084] 1.3.4 Liver tissue sections

[0085] The livers of the mice were sectioned, stained with he, and observed under an electron microscope at 200 times magnification. The pictures of each group are as Figure 2 shown.

[0086] By observing the liver tissue sections of each group of mice and analyzing the cell number, cell arrangement, and cell decay degree, it was found that the number of liver tissue cells in the negative group was significantly reduced compared with the blank group, the cell arrangement was disordered, and the cell decay degree was serious, indicating that there was an inflammatory condition in the liver tissue of the negative group. The number of cells in the positive drug group was increased compared with the negative control group, the arrangement was more compact and dense, and the decay condition was alleviated, indicating that the positive drug group had improved the liver tissue inflammation caused by alcohol. There were no significant changes in the liver tissue cells of the mice in Comparative Example 1 and Comparative Example 2 compared with the negative control group. However, Example 1 had varying degrees of improvement in terms of cell number, arrangement, and decay compared with the negative control group, indicating that Example 1 had an improvement effect on the liver tissue damage caused by alcohol and improved the damage of alcohol to the liver tissue cells of the mice.

[0087] 1.3.5 Determination of liver tissue homogenate indexes

[0088] The levels of TG, HDL-C, LDL-C and ALDH in liver tissues were detected according to the methods provided by the corresponding kits. The results are shown in Table 5. Compared with the blank control group, the levels of TG and LDL-C in the liver tissues of the negative group mice were significantly increased, while the levels of HDL-C and ALDH were significantly decreased. The levels of TG and LDL-C in the liver tissues of the negative group mice increased by 66.99% and 111.26% respectively, and the levels of HDL-C and ALDH decreased by 44.13% and 54.3% respectively. After the intervention with drugs and samples, the levels of TG, HDL-C, LDL-C and ALDH in the liver tissues of each group of mice changed to varying degrees. Among them, the changes in the levels of TG and ALDH in the Comparative Example 1 group and the Comparative Example 2 group were relatively small. The levels of HDL-C increased by 21.62% and 16.22% respectively, and the levels of LDL-C decreased by 18.07% and 30.12% respectively. However, the changes in each level of the positive drug group and Example 1 were significant. Compared with the negative group, the levels of TG in the liver tissues of the positive drug group and Example 1 mice decreased by 48.22% and 37.56% respectively, the levels of HDL-C increased by 40.54% and 54.05% respectively, the levels of LDL-C decreased by 59.04% and 46.99% respectively, and the levels of ALDH increased by 58.73% and 53.44% respectively. In summary, the liver tissue homogenate indexes showed that the effect of Example 1 was similar to that of the drug, with a significant liver protection effect, improving the effects of alcohol on lipid metabolism and the levels of alcohol metabolism-related enzyme activities in mouse liver tissues. Moreover, the improvement effect of Example 1 in each level was greater than the sum of Comparative Example 1 and 2, showing a strong synergistic effect.

[0089] Table 5 Results of liver tissue homogenate determination

[0090] Group TG (mmol / gprot) HDL-C (mmol / gprot) LDL-C (mmol / gprot) ALDH (U / mgprot) Blank 1.18±0.09 1.32±0.06 0.39±0.05 90.99±7.3 Negative 1.97±0.28 0.74±0.13 0.83±0.13 41.58±7.02 Positive 1.02±0.23 1.04±0.13 0.34±0.06 66±12.53 Example 1 1.23±0.3 1.14±0.16 0.44±0.1 63.8±7.51 Comparative Example 1 1.85±0.18 0.9±0.07 0.68±0.11 40.17±5.76 Comparative Example 2 1.78±0.23 0.86±0.11 0.58±0.11 38.75±6.27

[0091] The levels of SOD, MDA, GSH, and GSH-PX in liver tissues were detected according to the methods provided by the corresponding kits. The results are shown in Table 6. Compared with the blank control group, the level of MDA in the liver tissues of the negative group mice was significantly increased, while the levels of SOD, GSH, and GSH-PX were significantly decreased. The level of MDA in the liver tissues of the negative group mice increased by 313.44%, and the levels of SOD, GSH, and GSH-PX decreased by 60.73%, 32.37%, and 29.43% respectively; after intervention with the positive drug and the sample, the levels of SOD, MDA, GSH, and GSH-PX in the liver tissues of each group of mice were better improved. Among them, there were no significant changes in each level of the Comparative Example 1 group. The levels of SOD and GSH in Comparative Example 1 increased by 36.87% and 21.46% respectively, and the level of MDA decreased by 21.28%; while the changes in the positive drug and Example 1 were significant; compared with the negative group, the levels of MDA in the liver tissues of the positive drug and Example 1 mice decreased by 45.48% and 45.77% respectively, the level of SOD increased by 72.62% and 89.92% respectively, the levels of GSH increased by 32.54% and 33.10% respectively, and the levels of GSH-PX increased by 35.55% and 33.71% respectively. In summary, Example 1 has a significant effect on enhancing the antioxidant capacity of mouse liver tissues and shows a strong coordination effect in terms of the levels of SOD, MDA, and GSH-PX.

[0092] It shows that the substances in the Comparative Example 1 group and the Comparative Example 2 group have synergistic effects and enhance the antioxidant capacity of mouse liver tissues.

[0093] Table 6 Determination results of antioxidant indexes in liver tissues

[0094]

[0095]

[0096] 1.3.6 Western blot analysis of mouse liver proteins

[0097] Total liver proteins were extracted using RIPA lysis buffer and then denatured in a metal bath for 15 minutes. The protein concentration was determined using the BCA assay (Nanjing Jiancheng Bioengineering Institute). Then the total proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane at a constant voltage of 120V. Using Western blot analysis, the target proteins were detected with specific primary and secondary antibodies. The results are shown in Table 7 and Figure 3As shown, compared with the blank control group, the levels of TLR4, MyD88, and CYP2E1 in the liver tissues of mice in the negative group were significantly increased, while the levels of PPARα, Nrf2, and AMPK were significantly decreased. The levels of TLR4, MyD88, and CYP2E1 in the liver tissues of mice in the negative group were increased by 527.85%, 532.5%, and 589.8%, respectively, and the levels of PPARα, Nrf2, and AMPK were decreased by 87.78%, 88.08%, and 83.29%, respectively. Compared with the negative control group, the levels of TLR4, MyD88, and CYP2E1 in the positive drug and Example 1 were significantly decreased, while the levels of PPARα, Nrf2, and AMPK were significantly increased. The levels of TLR4 in the liver tissues of mice in the high-dose groups of the positive drug and Example 1 were decreased by 78.02% and 73.79%, respectively, the levels of MyD88 were decreased by 55.34% and 45.06%, respectively, the levels of CYP2E1 were decreased by 77.81% and 77.01%, respectively, the levels of PPARα were increased by 488.33% and 501.67%, respectively, the levels of Nrf2 were increased by 309.26% and 351.85%, respectively, and the levels of AMPK were increased by 224.64% and 227.54%, respectively. In this study, although the levels of TLR4, MyD88, and CYP2E1 in the groups of Comparative Example 1 and Comparative Example 2 were decreased to varying degrees compared with the negative control group, and the levels of PPARα, Nrf2, and AMPK were increased to varying degrees, the change ranges were all smaller than those of Example 1. In summary, Example 1 can improve the protein expression level in the liver tissues of mice.

[0098] Table 7 Results of Western blot of liver proteins

[0099] Group TLR4 PPARα Nrf2 MyD88 CYP2E1 AMPK Blank 0.26±0 1.64±0.01 1.51±0.04 0.13±0 0.16±0 1.38±0.04 Negative 1.65±0.01 0.2±0.01 0.18±0.01 0.84±0.02 1.13±0.02 0.23±0.01 Positive 0.82±0.02 0.9±0.02 0.3±0 0.25±0.01 0.29±0.01 0.42±0.02 Example 1 0.43±0.01 1.2±0.03 0.81±0.02 0.46±0.02 0.33±0.01 0.75±0.04 Comparative Example 1 0.89±0.01 0.98±0.01 0.38±0.01 0.33±0.01 0.38±0.01 0.69±0.02 Comparative Example 2 0.79±0.02 0.45±0 0.32±0.01 0.89±0.01 0.96±0.02 0.22±0.01

[0100] 1.3.7 Detection of intestinal microbiota in mice

[0101] Total DNA of intestinal microbiota was extracted from fecal samples, and 16S rDNA was amplified. Sequencing was performed on the Illumina MiSeq platform (Hangzhou Lianchuan Biotechnology Co., Ltd.) based on the latest version of QIIME2. The amplicon data was denoised using the Divisive Amplicon Denoising Algorithm (DADA2) and clustered to present 100% sequence identity. All results were based on features. The results are as Figures 4 - 11As shown, compared with the blank control group, the Alpha diversity of the intestinal flora of the mice in the negative control group was significantly reduced, and the Chao1 index, Ace index, Shannon index, and Simpson index were all significantly decreased. In contrast, the Alpha diversity of the positive drug and the high-dose group of Example 1 was significantly increased compared with that of the negative control group. By performing Beta diversity analysis on the intestinal flora of mice in each group, it can be concluded from the principal component analysis and principal coordinate analysis that there were significant species differences between the negative control group and the blank control group, and there were also significant species differences between the positive drug and the high-dose group of Example 1 compared with the negative control group. At the phylum level, compared with the blank control group, the relative abundance of Firmicutes in the negative control group increased by 52.4%, and the relative abundance of Bacteroidetes decreased by 61.8%. In contrast, compared with the negative control group, the intervention of the high-dose group of Example 1 reduced the abundance of Firmicutes by 42.6% and the abundance of Bacteroidetes by 219.1%. At the genus level, the relative abundances of Eisenbergiella and Akkermansia in the intestinal microbiota of mice in the negative control group were lower than those in the blank control group, while the abundances of Ligustrum and Desulfovibrio increased. On the contrary, compared with the negative control group, these changes in the mice of Example 1 were alleviated. This indicates that the intervention of the tea bag of Example 1 on the mice with alcoholic liver injury can improve the impact of alcohol on the intestinal flora of mice.

[0102] In summary, the hulless barley in Example 1 has a significant synergistic effect with other substances, and drinking the tea bag can reduce the levels of AST, ALT, ALP, and LDH in the serum, reduce the levels of serum inflammatory factors, improve the damage of alcohol to the liver tissue cells of mice, enhance the antioxidant capacity of the liver tissue of mice, improve the impact of alcohol on the levels of lipid metabolism and alcohol metabolism-related enzyme activities in the liver tissue of mice, and has a significant liver protection effect.

[0103] The embodiments provided above are not intended to limit the scope covered by the present invention, nor are the described steps intended to limit the order of their execution. Obvious improvements made by those skilled in the art to the present invention in combination with the existing common general knowledge also fall within the protection scope defined by the claims of the present invention.

Claims

1. A compound highland barley tea with the function of relieving hangover and protecting the liver, characterized in that, It is composed of the following raw materials in parts by weight: 50 - 70 parts of highland barley, 3 - 4 parts of kudzu root, 8 - 12 parts of rattan tea, 8 - 12 parts of red wolfberries, 2 - 4 parts of Hangzhou white chrysanthemum, and 0.2 - 0.4 parts of stevia.

2. The compound hulless barley tea according to claim 1, wherein The highland barley is the clean wheat obtained after screening, stone removal, wheat beating, wheat brushing, circulating air selection, and color sorting after being put into the warehouse.

3. The compound hulless barley tea according to claim 1, characterized in that, The highland barley is yellow highland barley.

4. The compound hulless barley tea according to claim 1, wherein The highland barley has been stir-fried by gradient. The stir-frying method is: mix the clean wheat with 8 - 10% water, pour it into a preheated wok and stir-fry by tumbling, then raise the temperature and continue stir-frying until the surface of the highland barley turns brownish-yellow, and then quickly pour it out of the wok and cool it to room temperature.

5. The compound hulless barley tea according to claim 4, wherein The preheating temperature is 225 - 235 °C, and the stir-frying time is 40 - 50 min.

6. The compound hulless barley tea according to claim 4, wherein Raise the temperature to 265 - 275 °C and stir-fry for 15 - 20 min.

7. The compound hulless barley tea according to claim 1, wherein The kudzu root, rattan tea, red wolfberries, Hangzhou white chrysanthemum, and stevia leaves are respectively air-dried naturally or dried at 50 - 70 °C until the moisture content is less than 10%, and then subjected to coarse crushing treatment with a mesh number of 5 - 15 meshes and reserved for use.

8. The preparation method of the compound highland barley tea according to claim 1, characterized in that, It includes the following steps: Mix 50 - 70 parts of highland barley, 3 - 4 parts of kudzu root, 8 - 12 parts of rattan tea, 8 - 12 parts of red wolfberries, 2 - 4 parts of Hangzhou white chrysanthemum, and 0.2 - 0.4 parts of stevia; use a teabag packaging machine for sub-packaging to obtain the bagged compound highland barley tea.

9. The preparation method according to claim 8, wherein The material of the tea bag is corn fiber.

10. The preparation method according to claim 8, characterized in that, The specification of the tea bag is 8.2 - 9 g / bag.

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