Mouth wash as well as preparation method and application thereof

Abstract

The invention discloses a novel plant component mouthwash. The mouthwash comprises eucalyptus leaf extract, tea leaf extract, sophocarpidine, cnidium lactone and the like. The mouthwash not only can effectively inhibit and resist bacteria, but also can clean the oral cavity, reduce tartar, refresh breath, reduce decayed teeth, prevent halitosis, prevent periodontal diseases and kill harmful germs, viruses and the like in oral mucosa; meanwhile, the mouthwash is prepared from plant extract components, so that the mouthwash is safe and harmless.

Description

Technical Field

[0001] The present invention relates to the technical field of oral care products, in particular to a mouthwash and its preparation method and application. Background Art

[0002] With the continuous improvement of the quality of life, more and more people begin to pay attention to oral health problems, and the vast majority of modern people realize the importance of oral health. Relevant survey data shows that about 92% of people in China have had oral problems, 92.3% of whom are women and 91.4% are men. Among them, more than 96% of people have had oral problems such as toothache and gum bleeding. It can be seen that oral health problems are increasingly affecting people's lives.

[0003] The oral cavity communicates directly with the outside world and the digestive tract, which can best reflect the state of human microorganisms and also best reflect specificity. Therefore, its status is particularly important. Oral microorganisms mostly form complex colonies in the form of acquired membranes in the oral cavity. The membrane structure mainly contains bacteria, fungi, mycoplasmas, viruses, etc. At present, more than 700 species in more than 40 genera of microorganisms have been understood and recognized, and the whole genome information of more than 400 species of microorganisms has been measured.

[0004] The physical and chemical properties and structures of different parts in the oral cavity are different, so the number of colonized microorganisms is not only large but also complex. It is detected that the number of microorganisms in saliva is 6×10 9 cells / mL, and the microorganisms in dental plaque are 5×10 11 cells / g. The microorganisms colonized in different parts are also different, and the dominant oral bacteria found in different diseases are also inconsistent.

[0005] At the same time, oral microbial disorders not only lead to oral diseases but also cause systemic diseases. The research on the correlation between the current dominant oral bacteria and Alzheimer's disease, diabetes, lichen planus and other systemic diseases has attracted increasing attention.

[0006] Ordinary brushing methods, to a large extent, cannot solve oral problems. In fact, when there are pathogenic bacteria in the oral cavity, brushing alone cannot solve the problem of sterilization. Then, the use of mouthwash came into being. However, due to the limitation of ingredients, the mouthwashes sold on the market either have poor sterilization effects or have certain safety problems. Therefore, there is an urgent need to research a new formula mouthwash with good sterilization effect and safe and harmless. Summary of the Invention

[0007] The purpose of the present invention is to provide a new mouthwash and its formula to solve the above problems.

[0008] According to the first aspect of the present invention, there is provided a mouthwash, which comprises the following components expressed by weight percentage:

[0009] Main ingredient A, 30 - 45%,

[0010] Ingredient B, 35 - 60%,

[0011] Ingredient C, 10 - 20%;

[0012] The main ingredient A includes the following components expressed by weight percentage:

[0013] Water, 44.43%,

[0014] Ethanol, 4%,

[0015] Essential oil, 1.15%,

[0016] Synergist, 1.05%,

[0017] Plant extract, 49%,

[0018] Phytocide, 0.37%;

[0019] The main component of the synergist is 0.05% thymol; the plant extract is 15% tea tree leaf extract and 34% eucalyptus leaf extract; the main components of the phytocide include 0.015 - 0.035% matrine and 0.015 - 0.035% osthole;

[0020] The ingredient B includes the following components expressed by weight percentage:

[0021] Erythritol, 1 - 7%,

[0022] Tea polyphenols, 0.1 - 1%,

[0023] Glycerol, 5 - 15%,

[0024] Essential oil, 0.1 - 1%,

[0025] Plant extract, 1 - 10%,

[0026] Purified water, balanced to 100%;

[0027] The ingredient C is purified water.

[0028] In some embodiments, the ratio of the main ingredient A is 30%, the ratio of the ingredient B is 50%, and the ratio of the ingredient C is 20%.

[0029] In some embodiments, the matrine is obtained by an alkaline alcohol extraction process from the dried root plants and fruits of Sophora flavescens juice, with a purity of 98%.

[0030] In some embodiments, the osthole is obtained by an organic solvent immersion extraction process from the fruits of Cnidium monnieri, with a purity of 98%.

[0031] In some embodiments, the essential oil is a plant essential oil with a purity of 99.9%.

[0032] In some embodiments, the plant essential oil is peppermint.

[0033] In some embodiments, the preparation process of the plant extract comprises the following steps:

[0034] 1) Put the plant raw materials into a distillation container for distillation;

[0035] 2) After condensing the distilled oil-water mixed gas, perform oil-water separation on the condensed effluent;

[0036] 3) Return the separated distillate water to the pot for continued distillation, and then separate the oil and water obtained from the re-distillation. The substances dissolved in the water are the obtained plant extract.

[0037] According to the second aspect of the present invention, a preparation method of a mouthwash is provided, and the preparation method comprises the following steps:

[0038] 1) First, add the components of main ingredient A;

[0039] 2) Then, add the components of ingredient B and stir evenly;

[0040] 3) Finally, add ingredient C and stir evenly;

[0041] 4) Inspect the prepared sample, and after passing the inspection, it can be packaged and completed.

[0042] According to the third aspect of the present invention, an application of a mouthwash in anti-Staphylococcus aureus is provided.

[0043] According to the fourth aspect of the present invention, an application of a mouthwash in inhibiting Staphylococcus aureus is provided.

[0044] Advantages of the present invention:

[0045] 1. The present invention discloses a new plant ingredient mouthwash, which can not only effectively inhibit bacteria and fungi, but also clean the oral cavity, reduce dental plaque, make the breath fresh, reduce dental caries, prevent bad breath, prevent periodontal disease, and kill harmful bacteria and viruses in the oral mucosa, etc.; at the same time, since the mouthwash is a plant extract ingredient, it is safe and harmless.

[0046] 2. The present invention also discloses a preparation method of a new plant ingredient mouthwash. The mouthwash prepared by this method has stable ingredients and better effects.

[0047] 3. The present invention also discloses the application of a new plant - ingredient mouthwash in anti - Staphylococcus aureus. According to the WS / T 650 - 2019 standard, when the bactericidal rate within the required specified time is ≥99%, it is judged to have a strong antibacterial effect. The average bactericidal rate of this mouthwash reaches 99.60%, which is higher than the requirement in the WS / T 650 - 2019 standard, indicating a strong antibacterial effect, especially against Staphylococcus aureus.

[0048] 4. The present invention also discloses the application of a new plant - ingredient mouthwash in inhibiting Staphylococcus aureus. According to the WS / T 650 - 2019 standard, when the bacteriostatic rate within the required specified time is ≥90%, it is judged to have a strong bacteriostatic effect. The average bacteriostatic rate of this mouthwash >99.98%, which is higher than the requirement in the WS / T 650 - 2019 standard, indicating a strong bacteriostatic effect, especially against Staphylococcus aureus. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 It is the process flow chart of the preparation of the mouthwash in the present invention;

[0050] Figure 2 It is the antibacterial test result of the mouthwash in the present invention;

[0051] Figure 3 It is the bacteriostatic test result of the mouthwash in the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0052] The invention will be further described in detail below in conjunction with the embodiments and the drawings.

[0053] Example 1 Composition and Preparation Process of the Mouthwash

[0054] I. Formula and Preparation of Main Ingredient A in the Mouthwash

[0055]

[0056] Remarks:

[0057] 1. Matrine is obtained from the dried root plants of Sophora flavescens juice, and the fruit is obtained through an alkaline alcohol extraction process. This product selects a finished product with a purity of 98%. Latin name of Sophora flavescens: Sophoraflavescens.

[0058] 2. Osthole is obtained from the fruit of Cnidium monnieri through an organic solvent immersion extraction process. This product selects a finished product with a purity of 98%. Latin name of Cnidium monnieri: Cnidiummonnieri(L)Cuss.

[0059] 3. Select different plant essential oils according to different products: For example, the Latin name of mint is Mentha. The extraction process of the essential oil is distillation extraction method, and the purity of the obtained essential oil is 99.9%. The oil yield of the plant by weight is about 50-90:1.

[0060] 4. The total ratio of all the main components 1, 2, 3, 4, 5, and 6 in this formula is 100%. The percentage content of the active ingredients (4.1, 5.1, 5.2, 6.1, 6.2) in the components only indicates their dosage and percentage in this formula. Do not repeat the cumulative calculation during calculation.

[0061] Preparation process of plant extracts (the extraction processes of tea leaves and eucalyptus leaves are the same. The following takes eucalyptus leaves as an example for illustration):

[0062] 1. Treatment of eucalyptus leaf raw materials;

[0063] Pick fresh eucalyptus leaves in batches and divide them well. It is best to process them directly to avoid direct sunlight.

[0064] 2. Selection of distillation equipment;

[0065] Distillation pot: The diameter of the pot body is 86 cm, the height is 100-110 cm, and it can hold about 130-140 kg of eucalyptus leaves.

[0066] Condenser: A shell-and-tube or spiral-tube condenser can be selected, and the condensation area is about 1.5-2.0 square meters.

[0067] Oil-water separator: The diameter is about 20 cm and the height is 20 cm.

[0068] 3. Distillation:

[0069] 1) Loading the pot: Before loading the eucalyptus leaves into the pot, check whether the equipment is completely connected and normal. If there are any leakage points, repair them as soon as possible. After ensuring that the equipment is airtight, the eucalyptus leaves can be loaded into the pot. When loading, the tightness should be appropriate, and the periphery of the pot should be slightly tightened. The loading should not be too full. Generally, it can be loaded to 70%-80%.

[0070] 2) Distilling After loading the eucalyptus leaves into the pot, cover the pot lid and connect the relevant pipes. Then, ignite the distillation. The oil-water mixed gas is steamed out of the pot and enters the condenser through the connecting pipe for cooling. The condensed effluent is separated for oil and water. The separated distillate water is returned to the pot for distillation. After further distillation of the eucalyptus oil, try to distill out the high-boiling components in the oil (the high-boiling components in the eucalyptus oil usually contain some specific active substances, such as certain terpene compounds or other functional components. Through further distillation, these high-boiling components can be more effectively separated and enriched, thereby improving the purity of the eucalyptus oil and enabling it to proceed smoothly in the following process).

[0071] 3) Oils lighter than water will float on the water surface, and the remaining water is the plant extract (eucalyptus leaf extract). Then, use a separating funnel to further store the eucalyptus leaf extract.

[0072] According to the same process as above, tea leaf extract can be obtained.

[0073] The yield rate of the plant extract is generally between 85% - 96%, which varies slightly according to the degree of operation loss.

[0074] II. Formula of Mouthwash

[0075]

[0076]

[0077]

[0078] Example II Preparation Process of Mouthwash

[0079] I. Production Equipment: Stainless steel reaction tank, etc.

[0080] II. Process flow chart is shown in the appendix Figure 1 。

[0081] III. Key control points of operation:

[0082] 1. Weighing: Master the large and small weighing scales and accurately weigh each material according to the formula quantity;

[0083] 2. Add in the order specified by the process operation;

[0084] 3. Production: The melting temperature is normal temperature.

[0085] IV. Operating procedures:

[0086] Phase A: Main ingredient A (Main ingredient A in Table 1 - 3);

[0087] Phase B: Mouthwash matrix (Ingredient B in Table 2 or 3);

[0088] Phase C: Purified water (Ingredient C in Table 2 or 3).

[0089] After the equipment is sterilized and disinfected, start working.

[0090] 1. Add the components of Phase A of the main ingredient (Table 1);

[0091] 2. Add Ingredient B (add it once only), and finally stir evenly;

[0092] 3. Add Ingredient C (add it once only), and finally stir evenly.

[0093] 4. After stirring evenly, send it to the laboratory for inspection. After meeting the industry standards and the internal control standards of this enterprise, it can be discharged only with the qualified release note from the laboratory.

[0094] 5. Check the physical and chemical indicators, and fill it after standing for 24 hours to defoam.

[0095] 6. Take samples for micro-inspection, and store it in the warehouse after passing the inspection.

[0096] V. Finished product quality standards

[0097] After the finished product is packaged, it shall be inspected with reference to the requirements for the packaging appearance of disinfection products.

[0098] The quality inspection items of the mouthwash are shown in Table 4:

[0099]

[0100] Example 3. Antibacterial function detection of mouthwash

[0101] I. Instructions for use of mouthwash

[0102] 1. Instructions for use:

[0103] 1) It is recommended to use it after brushing your teeth or when you need to clean your mouth.

[0104] 2) It is recommended to use 5 - 15 ml each time, gargle continuously for 60 seconds and then spit it out.

[0105] 2. Product efficacy:

[0106] 1) Sterilize comprehensively to prevent oral problems.

[0107] 2) Purify the odor and keep fresh for a long time.

[0108] 3. Storage method:

[0109] 1) Store it in a cool and dry place.

[0110] 4. Precautions:

[0111] 1) Do not swallow it, and children under 12 years old are not suitable to use it.

[0112] II. Antibacterial function detection of mouthwash

[0113] The finished mouthwash is sent to the Guangdong Academy of Sciences Testing and Analysis Institute (China National Analytical Center, Guangzhou) for testing, and the test results are as Figure 1 and Figure 2 shown.

[0114] As Figure 1The detection results show that the mouthwash in the present invention has a strong antibacterial effect on Staphylococcus aureus. With an action time of 30 s, the average sterilization rate is as high as 99.60%, far higher than the requirement of ≥99% specified in the WS / T 650-2019 standard, indicating a strong antibacterial effect, especially on Staphylococcus aureus.

[0115] Such as Figure 2 The detection results show that the mouthwash in the present invention has a strong bacteriostatic effect on Staphylococcus aureus. With an action time of 1 h, the average bacteriostatic rate >99.98%, far higher than the requirement of ≥90% specified in the WS / T 650-2019 standard, indicating a strong bacteriostatic effect, especially on Staphylococcus aureus.

[0116] The above are only some embodiments of the present invention. For those of ordinary skill in the art, without departing from the inventive concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the invention.

Claims

1. A mouthwash, wherein, The mouthwash comprises the following components expressed as weight percentages: Main ingredient A, 30-45%, Ingredient B, 35-60%, Ingredient C, 10-20%; The main ingredient A comprises the following components expressed as weight percentages: Water, 44.43%, Ethanol, 4%, Essential oil, 1.15%, Synergist, 1.05%, Plant extract, 49%, Phytocide, 0.37%; The main component of the synergist is 0.05% thymol; the plant extract is 15% tea tree leaf extract and 34% eucalyptus leaf extract; the main components of the phytocide include 0.015-0.035% matrine and 0.015-0.035% osthole; The ingredient B comprises the following components expressed as weight percentages: Erythritol, 1-7%, Tea polyphenols, 0.1-1%, Glycerol, 5-15%, Essential oil, 0.1-1%, Plant extract, 1-10%, Purified water, balanced to 100%; The ingredient C is purified water.

2. The mouthwash according to claim 1, wherein, The ratio of the main ingredient A is 30%, the ratio of the ingredient B is 50%, and the ratio of the ingredient C is 20%.

3. The mouthwash according to claim 1, wherein, The matrine is obtained by an alkaline alcohol extraction process using the dried root plants and fruits of Sophora flavescens juice, with a purity of 98%.

4. The mouthwash according to claim 1, wherein, The osthole is obtained by an organic solvent soaking extraction process using the fruits of Cnidium monnieri, with a purity of 98%.

5. The mouthwash according to claim 1, wherein, The essential oil is a plant essential oil with a purity of 99.9%.

6. The mouthwash according to claim 5, wherein, The plant essential oil is peppermint.

7. The mouthwash according to claim 1, wherein, The preparation process of the plant extract comprises the following steps: 1) Put the plant raw materials into a distillation container for distillation; 2) After condensing the distilled oil-water mixed gas, perform oil-water separation on the condensed effluent; 3) Return the separated distillate water to the pot for continuous distillation, and then separate the oil and water obtained from the re-distillation. The substances dissolved in the water are the obtained plant extract.

8. The preparation method of the mouthwash according to any one of claims 1-7, wherein, The preparation method comprises the following steps: 1) First add the components of the main ingredient A; 2) Then add the components of the ingredient B and stir evenly; 3) Finally add the ingredient C and stir evenly; 4) Inspect the prepared sample, and after passing the inspection, it can be packaged and completed.

9. Use of the mouthwash according to any one of claims 1-7 in anti-Staphylococcus aureus.

10. Use of the mouthwash according to any one of claims 1-7 in inhibiting Staphylococcus aureus.

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