Anti-oxidation composition, anti-oxidation soothing cream and preparation method of anti-oxidation soothing cream

Abstract

The invention relates to the technical field of cream, in particular to an antioxidant composition, antioxidant soothing cream and a preparation method thereof. The antioxidant composition comprises the following raw materials: a mangnolia officinalis bark extract and a grape seed extract. According to the present invention, the cortex magnoliae officinalis extract and the grape seed extract are adopted as the composition according to the specific ratio so as to achieve the synergistic effect, and the antioxidant stress regulation switch Nrf2 can be opened so as to open the antioxidant stress regulation signal channel of the cell, translocate the Nrf2 to the cell nucleus, activate the ARE, activate the transcription of the antioxidant gene, and improve the antioxidant activity of the antioxidant gene; and the anti-oxidative stress system of the body is started, and anti-oxidation is carried out from an internal source.

Description

Technical Field

[0001] The present invention relates to the technical field of creams, and in particular to an antioxidant composition, an antioxidant soothing cream and a preparation method thereof. Background Art

[0002] The skin is the largest organ in the human body. Since birth, the human skin tissue has become increasingly developed and its functions have gradually improved. It is generally considered to reach its peak at the age of 25 and then gradually ages. There are many factors causing aging. Besides genetic factors, oxidative stress from the external environment that causes cells to be oxidized is one of the most important reasons. For example, greater work pressure, air pollution, sunlight, and ultraviolet radiation will all exacerbate the generation of free radicals in the skin, damaging the cell, protein and other structures of the human skin.

[0003] Generally, the oxidation of the skin is an inevitable and irreversible natural process, that is, the above-mentioned damage cannot be repaired. Therefore, the human skin will show aging phenomena such as dullness, sagging, and wrinkles. However, some people will have abnormal immune functions due to excessive or extreme oxidative reactions, resulting in an overreaction of the immune system (allergic reaction), such as drug allergy or contact allergy. If such phenomena occur frequently, it is called sensitive skin. Sensitive skin is caused by the damage of the skin surface barrier, resulting in a decline in skin immunity. If these damaged cells are not repaired in time, these cells will continuously divide into more skin cells with immune defects or damaged sebum membrane secretion, thus causing more sequelae, such as long-term skin allergy leading to skin damage, causing skin redness, pigmentation, acne, enlarged pores, skin spots, aging wrinkles, etc.

[0004] Worse still, these allergic reactions will further lead to inflammatory aging (inflammaging). So-called inflammatory aging is a phenomenon of premature aging, and its essence is that a chronic inflammation exacerbates the aging process. Inflammation is what is usually referred to as "inflammation", which is a defense reaction of the body to stimuli (such as PM2.5, drugs or other pathogens, etc.), manifested as redness, swelling, heat, pain and dysfunction. Usually, it is an active defense reaction of the human body, and this reaction can be divided into a pro-inflammatory reaction and an anti-inflammatory reaction. When the pro-inflammatory reaction and the anti-inflammatory reaction develop in balance, the inflammation can clear the infection and then subside, allowing normal tissues to be rebuilt. This process will not show the phenomenon of premature aging, and it is also called healthy aging. When the reactions of the two are unbalanced, for example, the infection has not completely subsided, or the inflammatory reaction becomes very sensitive or continuously affected by low irritation, the inflammation will persist and continuously release free radicals, continuously damaging the skin, and thus triggering a series of diseases related to chronic inflammation, accelerating skin aging, and even leading to the occurrence of skin cancer.

[0005] At present, such sensitive skin problems and inflammatory aging phenomena have attracted more and more attention from consumers and the medical community. People have an increasing demand for skin antioxidant, and reducing the generation of free radicals, scavenging aging metabolites, and increasing the activity of antioxidant enzymes have become effective methods for delaying skin aging. Various antioxidant products have been developed on the market, but the existing products have poor antioxidant effects, and there is no method for producing antioxidant products with magnolia bark extract and grape seed extract as a composition. There are also few products that can activate the pathway of the body's own antioxidant stress system and conduct antioxidant from the endogenous level. Summary of the Invention

[0006] The main object of the present invention is to provide an antioxidant composition, an antioxidant soothing cream and a preparation method thereof, aiming to improve the technical problems that the existing antioxidant products have poor antioxidant effects or cannot activate the activity of the Nrf2-ARE pathway of the body's own antioxidant stress system and conduct antioxidant from the endogenous level.

[0007] To achieve the above object, the present invention provides an antioxidant composition, which comprises the following raw materials: magnolia bark extract and grape seed extract.

[0008] Preferably, the concentration ratio of the magnolia bark extract to the grape seed extract is 1:1-11.

[0009] The present invention also provides an antioxidant soothing cream, and by total weight percentage of the composition, the antioxidant soothing cream comprises the following components:

[0010] Phase A components: humectant 1-20%, first preservative 0-1.5%, first thickener 0-1% and solvent 30-90%;

[0011] Phase B components: first emulsifier 1-6% and first emollient 5-40%;

[0012] Phase C components: second thickener 0.2-5%;

[0013] Phase D components: third thickener 0.1-5%, soother 0.5-10%, second emulsifier 0-2%, second emollient 0-1.2%, penetration enhancer 0-0.5%, second preservative 0-2.55% and fragrance 0-0.05%; by weight percentage, the soother in the Phase D components comprises the antioxidant composition, wherein the magnolia bark extract contains 1-5% and the grape seed extract contains 1-5%.

[0014] Preferably, by weight percentage, the soother in the Phase D components further comprises 0-0.5% of dipotassium glycyrrhizinate;

[0015] The third thickener in the Phase D components is maltodextrin, the second emulsifier is lecithin, and the penetration enhancer is ethanol.

[0016] Preferably, by weight percentage, the second emollient in the D-phase component comprises the following raw materials: 0-1.2% of vitamin E and 0-1.2% of PEG-40 hydrogenated castor oil; the second preservative in the D-phase component comprises the following raw materials: 0-1.2% of phenoxyethanol, 0-0.5% of ethylhexylglycerin, 0-1.5% of triethylene glycol, the total amount of bis(hydroxymethyl) imidazolidinyl urea and iodopropynyl butylcarbamate is 0-0.3%, and 0-0.2% of hydroxyacetophenone.

[0017] Preferably, by weight percentage, the humectant in the A-phase component comprises the following raw materials: 1-5% of butanediol, 1-5% of propylene glycol, 1-8% of glycerol, and 1-5% of sorbitol.

[0018] Preferably, by weight percentage, the first thickener in the A-phase component comprises the following raw materials: 0-1% of hydroxyethyl cellulose, 0.05-0.2% of carbomer, 0.01-0.2% of xanthan gum, and 0.02-0.2% of triethanolamine;

[0019] The first preservative in the A-phase component is disodium EDTA, and the solvent is water.

[0020] Preferably, by weight percentage, the first emulsifier in the B-phase component comprises the following raw materials: 0.5-5% of olive oil unsaponifiables, 1-5% of sucrose poly stearate, 0.5-5% of coco-glucoside, 0.5-5% of glyceryl stearate, and 0.5-5% of PEG-100 stearate.

[0021] Preferably, by weight percentage, the first emollient in the B-phase component comprises the following raw materials: 1-5% of stearic acid, 1-7% of shea butter, 1-5% of ethylhexyl hydroxystearate, 1-6% of jojoba seed oil, 0.5-5% of cetyl alcohol, 0.5-5% of cetearyl alcohol, 1-5% of polydimethylsiloxane, and 1-15% of cyclomethicone.

[0022] Preferably, the second thickener in the C-phase component is polyacrylamide(and)C 13-14 isoparaffin(and)laureth-7.

[0023] In addition, the present invention also provides a preparation method of the above antioxidant soothing cream, comprising the following preparation steps:

[0024] S1. Mix the humectant with the xanthan gum or the hydroxyethyl cellulose evenly, then add them into the solvent, then add the carbomer, and finally add the triethanolamine and the first preservative and mix evenly to obtain the A-phase component;

[0025] S2. After mixing the first emulsifier and the first emollient evenly to obtain the B-phase component, heat the A-phase component and the B-phase component in a water bath to 65-75 °C respectively, add the heated B-phase component to the A-phase component and emulsify and homogenize for 1-5 min; then add the second thickener and homogenize for 1-5 min; after cooling to ≤45 °C, add the third thickener, the soothing agent, the second emulsifier, the second emollient, the penetration enhancer, the second preservative and the fragrance in sequence and stir evenly.

[0026] Compared with the prior art, the antioxidant composition, the antioxidant soothing cream and the preparation method thereof of the present invention have the following beneficial effects: In the antioxidant soothing cream product of this solution, magnolia bark extract and grape seed extract are used as a composition to achieve a synergistic effect, which can turn on the antioxidant stress regulation switch Nrf2, so that the cells turn on the antioxidant stress regulation signal pathway, make Nrf2 translocate to the nucleus, activate ARE, so as to activate the transcription of antioxidant genes and start the body's own antioxidant stress system to carry out antioxidant from the inside source. Brief Description of the Drawings

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, other related drawings can also be obtained based on these drawings without creative efforts.

[0028] Figure 1 The lipid peroxidation inhibition rate of grape seed extract at different concentrations (0.1%, 0.5% and 2.5%);

[0029] Figure 2 The influence of different extracts and combinations on the Nrf2 level in HaCaT cells. Among them, A is the total protein level of Nrf2 immunoblotting, and B is the quantitative analysis chart of the Nrf2 protein level;

[0030] Figure 3 The influence of different extracts and combinations on the ARE activity in HaCaT cells;

[0031] Figure 4 The inhibition rate of the composition of magnolia bark extract and grape seed extract on COX at different concentrations;

[0032] Figure 5 The ARE activity test of the composition of magnolia bark extract and grape seed extract at different concentration combinations;

[0033] Figure 6This is a comparison chart before and after applying the antioxidant soothing cream in the skin improvement effect experiment of this solution;

[0034] Figure 7 This is a comparison chart before and after applying the soothing cream in the experiment of reducing the degree of redness and swelling of this solution. Among them, the first picture is before microneedle skin puncture, the second picture is after microneedle skin puncture, and the third picture is 15 minutes after applying the soothing cream.

[0035] The realization, functional characteristics and advantages of the purpose of this application will be further described with reference to the embodiments and the accompanying drawings. Detailed implementation manners

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0037] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions appears to be contradictory or unable to be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present invention.

[0038] An antioxidant soothing cream, calculated by the total weight percentage of the composition, the antioxidant soothing cream includes the following components:

[0039] Phase A components: humectant 1-20%, first preservative 0-1.5%, first thickener 0-1% and solvent 30-90%; wherein, the humectant includes the following raw materials: butylene glycol 1-5%, propylene glycol 1-5%, glycerol 1-8% and sorbitol 1-5%; the first thickener includes the following raw materials: hydroxyethyl cellulose 0-1%, carbomer 0.05-0.2%, xanthan gum 0.01-0.2% and triethanolamine 0.02-0.2%; the first preservative may specifically be disodium EDTA, and the solvent may specifically be water;

[0040] Phase B components: first emulsifier 1-6% and first emollient 5-40%; wherein, the first emulsifier includes the following raw materials: olive oil unsaponifiables 0.5-5%, sucrose poly stearate 1-5%, cocoyl glucoside 0.5-5%, glycerol stearate 0.5-5% and PEG-100 stearate 0.5-5%; the first emollient includes the following raw materials: stearic acid 1-5%, shea butter 1-5%, ethylhexyl olivate 1-5%, jojoba seed oil 1-5%, cetyl alcohol 0.5-5%, cetearyl alcohol 0.5-5%, polydimethylsiloxane 1-5% and cyclomethicone 1-15%;

[0041] Phase C components: 0.2 - 5% of the second thickener; the second thickener is polyacrylamide (and) C 13-14 Isoparaffin (and) laureth - 7;

[0042] Phase D components: 0.1 - 5% of the third thickener, 0.5 - 10% of the soothing agent, 0 - 2% of the second emulsifier, 0 - 1.2% of the second emollient, 0 - 0.5% of the penetration enhancer, 0 - 2.55% of the second preservative, and 0 - 0.05% of the fragrance; among them, by weight percentage, the soothing agent includes the following raw materials: (antioxidant composition) 1 - 5% of Magnolia officinalis bark extract, 1 - 5% of grape seed extract, and 0 - 0.5% of dipotassium glycyrrhizinate; the third thickener is maltodextrin, the second emulsifier is lecithin, and the penetration enhancer is ethanol; the second emollient includes the following raw materials: 0 - 1% of vitamin E and 0 - 0.2% of PEG - 40 hydrogenated castor oil; the second preservative includes the following raw materials: 0 - 1.2% of phenoxyethanol, 0 - 0.5% of ethylhexylglycerin, 0 - 1.5% of triethylene glycol, the total amount of bis(hydroxymethyl) imidazolidinyl urea and iodopropynyl butylcarbamate is 0 - 0.3%, and 0 - 0.2% of p - hydroxyacetophenone. And in the antioxidant composition, the concentration ratio of the Magnolia officinalis bark extract to the grape seed extract is 1:1 - 11.

[0043] Nrf2 is a transcription factor that plays an important role in cells. The Nrf2 protein structure consists of multiple functional domains, which are involved in biological processes such as transcriptional regulation, gene expression, and antioxidant stress response of the Nrf2 protein. Nrf2 plays a key role in the cellular oxidative stress response and is the regulatory switch for cells to initiate the antioxidant stress response, protecting cells from the harm of oxidative stress. By interacting with the antioxidant response element (ARE), it regulates the expression of antioxidant proteins and phase II detoxifying enzymes, thereby protecting cells from oxidative stress damage. The Nrf2 signaling pathway plays a key role in the occurrence and development of various diseases, especially in antioxidant stress and inflammatory responses. Studies have shown that the activation of the Nrf2 signaling pathway can enhance the antioxidant capacity of cells, reduce nerve cell damage caused by oxidative stress, and protect cells from diseases such as inflammation and tumors. Of course, the Nrf2 signaling pathway also plays a key role in the physiological activities of skin health. In the study of skin fibrosis, the deletion of Nrf2 in fibroblasts may lead to an increase in the expression of cytokines and chemokines, exacerbating the skin inflammatory response and thus promoting the occurrence of skin fibrosis. There are also studies indicating that Nrf2 may play a role in skin fibroblasts by regulating genes related to the extracellular matrix (ECM), and the downregulation of its expression will affect the deposition of ECM, which emphasizes the potential role of Nrf2 in maintaining skin homeostasis and protecting against aging. It is shown that in the context of skin fibrosis, the activity of Nrf2 may need to be upregulated to exert its protective effect. In addition, maintaining the normal expression of Nrf2 is important for the function of skin fibroblasts and the health status of the skin.

[0044] In existing products, ingredients with anti-inflammatory and antioxidant effects such as grape seed extract (hereinafter referred to as GSP-T), chalcone (hereinafter referred to as Chalcone), and magnolia extract (hereinafter referred to as Magnolia) are used. Although the above ingredients have been mentioned in some studies to activate the activity of Nrf2, not all of them can enable Nrf2 to translocate smoothly to the nucleus and combine with the antioxidant response element (ARE) to activate the transcription of antioxidant genes. This process is crucial for truly activating the body's own antioxidant stress system. When Nrf2 is activated, it translocates to the nucleus and binds to the ARE (antioxidant response element) of the target gene through heterodimerization, thus opening an antioxidant channel that starts inside the nucleus and goes from the inside out. ARE is a cis-regulatory element of a specific DNA sequence present in the upstream regulatory region of genes encoding detoxifying enzymes and cytoprotective proteins. Activating ARE can regulate the expression of detoxifying enzymes and structural proteins, which are crucial for metabolism, detoxification, redox homeostasis, and cell survival, and can induce the expression of a series of cytoprotective genes, such as NAD(P)H: quinone oxidoreductase 1 (NQO1), glutathione S-transferase (GST), heme oxygenase 1 (HMOX1), glutathione synthetase (GCL), glutathione GSH, etc. These genes play an important role in the cell's resistance to antioxidant stress and maintenance of cell health. Therefore, simply activating Nrf2 is not enough. Ensuring its effective entry into the nucleus and binding to ARE is the key to achieving antioxidant protection. In the antioxidant soothing cream product of this solution, magnolia bark extract and grape seed extract are used as a composition to achieve a synergistic effect, turn on the antioxidant stress regulation switch Nrf2, so that the cell turns on the antioxidant stress regulation signaling pathway, enables Nrf2 to translocate to the nucleus, activates ARE, and activates the transcription of antioxidant genes, thereby starting the body's own antioxidant stress system.

[0045] In addition, the combination of grape seed extract (GSP-T) + Magnolia extract is used to activate Nrf2, which can not only ensure the redox homeostasis of cells, exert anti-inflammatory and anti-allergic effects, but also drive the oxidative stress effect of the autophagy-lysosome pathway. Autophagy is a physiological process in the body for cell metabolism, clearing damaged cell debris or toxic metabolic molecules, including oxidized damaged proteins and dysfunctional mitochondria, etc. It is a strictly regulated cell degradation pathway or a process of self-detoxification and metabolism. The combination of GSP-T + Magnolia can also inhibit cyclooxygenase (COX). COX, also known as prostaglandin endoperoxide synthase (PTGS), is an important enzyme in the body. Two cyclooxygenase subtypes, called COX-1 and COX-2, have been identified. When cells or organisms are damaged, a series of inflammatory reactions will occur. When inflammation occurs, the expression of COX increases significantly throughout the body, thus causing inflammation, swelling, fever, pain, etc. everywhere. Therefore, by inhibiting the level of COX, this solution can play a role in reducing redness, relieving fever, analgesia, and anti-inflammation to a certain extent.

[0046] In addition, the present invention also provides a method for preparing the above antioxidant soothing cream, which includes the following preparation steps:

[0047] S1. Mix the moisturizer with xanthan gum or hydroxyethyl cellulose evenly, then add them to a solvent, then add carbomer, and finally add triethanolamine and the first preservative and mix evenly to obtain the A-phase component;

[0048] S2. Mix the first emulsifier and the first emollient evenly to obtain the B-phase component. Then heat the A-phase component and the B-phase component in a water bath to 65-75 °C respectively. Add the heated B-phase component to the A-phase component and emulsify and homogenize for 1-5 minutes; then add the second thickener and homogenize for 1-5 minutes; cool down

[0049] After cooling to ≤45 °C, add the third thickener, the soothing agent, the second emulsifier, the second emollient, the penetration enhancer, the second preservative and the fragrance and stir evenly in sequence.

[0050] The antioxidant soothing cream obtained by the above special preparation method has all components evenly dispersed in the cream. By adding different types of thickeners in batches, the synergistic effect of each component is better, and the performance of the antioxidant soothing cream is improved.

[0051] The technical solutions of the present invention will be further described in detail below with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0052] Example 1

[0053] This protocol studied the effects of different extracts on the Nrf2 protein level in HaCaT cells (human immortalized keratinocytes), and immunoblot analysis was performed using Nrf2 antibody to study the changes in the expression level of Nrf2 protein in HaCaT cells treated with single extracts and combinations of multiple different extracts.

[0054] The results showed that: as Figures 1 to 2 shown, treatment with a single extract, even when its inhibition rate of lipid peroxidation reached 90% at a concentration of 2.5%, had no significant effect on the Nrf2 protein level. However, as Figure 2 A and Figure 2 B shown, compared with when each extract was used alone, when the extracts were combined, namely GSP-T + Chalcone, GSP-T + Magnolia, Chalcone

[0055] + Magnolia, and GSP-T + Chalcone + Magnolia, respectively led to a significant increase in the Nrf2 expression level by 110%, 65%, 83% and 91%. These results indicate that a single extract cannot significantly increase Nrf2 expression, and only by combining these antioxidant extracts, GSP-T + Chalcone, GSP-T + Magnolia, and GSP-T + Chalcone + Magnolia can the Nrf2 expression be increased and the first switch of the endogenous antioxidant pathway be turned on.

[0056] Example 2

[0057] This protocol also combined the extracts and used a dual-luciferase assay kit to test the ARE transcriptional activity in HaCaT cells after 8 hours of treatment with different combinations of extracts.

[0058] As Figure 3 shown, the results showed that each extract had no significant effect on the ARE level when used alone. However, when the extracts were combined, especially after treatment with the combination of GSP-T (grape seed extract) + Magnolia (magnolia bark extract) ( Figure 3 the eighth group from left to right in

[0059] Example 3

[0060] This protocol also used a COX inhibitor screening colorimetric method to study the inhibitory efficacy of the GSP-T + Magnolia combination on COX.

[0061] As Figure 4 shown, when the total concentration of the composition ≤ 0.05%, relevant data could not be detected. When the total concentration of the composition was 0.1%, the inhibition rate had reached approximately 50%. When the total concentration of the composition increased to 1%, the inhibition rate reached approximately 100%. That is to say, when the inflammatory reaction occurred, the GSP-T + Magnolia composition could almost completely inhibit cyclooxygenase (COX), thereby achieving an obvious anti-inflammatory effect.

[0062] Example 4

[0063] To detect the optimal concentration ratio of the GSP-T + Magnolia composition, this protocol tested the ARE transcriptional activity of HaCaT cells after 8 hours of combined treatment at different extract concentration ratios (the concentration ratio of GSP-T to Magnolia was 1:1, 1:1.5, 1:5, 1:11).

[0064] As Figure 5 shown ( Figure 5 only partial data are listed), when the ratio of Magnolia to GSP-T was 1:1 - 11, the activity of ARE was improved to varying degrees. Among them, when the ratio of Magnolia to GSP-T was 1:1, the activity of ARE increased significantly by one time, that is, the antioxidant effect was the best at this concentration.

[0065] Comparative Example 1

[0066] Commercially available conventional antioxidant soothing cream.

[0067] Example 5

[0068] A method for preparing an antioxidant soothing cream, comprising the following preparation steps:

[0069] S1. Mix 9% of a humectant (specifically including 5% butylene glycol and 4% propylene glycol) with 0.05% of a first thickener (xanthan gum) evenly, then add it to 54.49% of a solvent (water), then add 0.1% of a first thickener (carbomer), and finally add 0.02% of a first thickener (triethanolamine) and 0.05% of a first preservative (EDTA disodium) and mix evenly to obtain the A-phase component;

[0070] S2. Mix 4% of the first emulsifier (specifically including 1% of olive oil unsaponifiables, 1% of sucrose poly stearate, 1% of coco-glucoside, 0.5% of glyceryl stearate, and 0.5% of PEG-100 stearate) and 25% of the first emollient (specifically including 1% of stearic acid, 2% of shea butter, 1% of ethylhexyl olivate, 3% of jojoba seed oil, 1% of cetyl alcohol, 1% of cetearyl alcohol, 1% of polydimethylsiloxane, and 15% of cyclomethicone) evenly to obtain the B-phase component. Then, heat the A-phase component and the B-phase component in a water bath to 70 °C, add the heated B-phase component to the A-phase component, and emulsify and homogenize for 3 min; then add 0.8% of the second thickener (polyacrylamide (and) C 13-14 isoparaffin (and) laureth-7) and homogenize for 1 min; after cooling to ≤45 °C, add 0.1% of the third thickener (maltodextrin), 4.0% of the soothing agent (specifically including 2% of magnolia bark extract and 2% of grape seed extract), 0.7% of the second emulsifier (0.2% of lecithin, 0.5% of PEG-40 hydrogenated castor oil), 0.02% of the second emollient (vitamin E), 0.05% of the penetration enhancer (ethanol), 1.57% of the second preservative (specifically including 0.5% of phenoxyethanol, 0.02% of ethylhexylglycerin, 1% of triethylene glycol, the total amount of bis(hydroxymethyl)imidazolidinone and iodopropynyl butylcarbamate is 0.03%, and 0.02% of p-hydroxyacetophenone), and 0.05% of the fragrance and stir evenly.

[0071] Example 6

[0072] A method for preparing an antioxidant soothing cream, comprising the following preparation steps:

[0073] S1. Mix 12% of the humectant (5% of butanediol, 3% of propylene glycol, 3% of glycerol, and 1% of sorbitol) with 0.15% of the first thickener (xanthan gum) evenly, add it to 43.31% of the solvent (water), then add 0.1% of the first thickener (carbomer), and finally add 0.05% of the first thickener (triethanolamine) and 0.05% of the first preservative (EDTA disodium) and mix evenly to obtain the A-phase component;

[0074] S2. Mix 5% of the first emulsifier (1% of olive oil unsaponifiables, 1% of sucrose poly stearate, 1% of coco glucoside, 1% of glyceryl stearate, and 1% of PEG - 100 stearate) and 30% of the first emollient (2% of stearic acid, 7% of shea butter, 1% of ethylhexyl olivate, 6% of jojoba seed oil, 2% of cetyl alcohol, 2% of cetearyl alcohol, 5% of dimethicone, and 5% of cyclomethicone) evenly to obtain the B - phase component. Then, heat the A - phase component and the B - phase component in a water bath to 75°C respectively, and add the heated B - phase component to the A - phase component for emulsification and homogenization for 2 min; then add 1% of the second thickener (polyacrylamide (and) C 13-14 isoparaffin (and) laureth - 7) for homogenization for 3 min; after cooling to ≤45°C, add 0.5% of the third thickener (maltodextrin), 4.5% of the soothing agent (2% of magnolia bark extract, 2% of grape seed extract, and 0.5% of dipotassium glycyrrhizinate), 0.2% of the second emulsifier (lecithin), 0.4% of the second emollient (0.2% of vitamin E and 0.2% of PEG - 40 hydrogenated castor oil), 0.2% of the penetration enhancer (ethanol), 2.5% of the second preservative (1% of phenoxyethanol, 0.1% of ethylhexylglycerin, 1% of triethylene glycol, the total amount of bis (hydroxymethyl) imidazolidinyl urea and iodopropynyl butylcarbamate is 0.3%, and 0.1% of p - hydroxyacetophenone), and 0.04% of the fragrance and stir evenly.

[0075] Example 7

[0076] A preparation method of an antioxidant soothing cream, comprising the following preparation steps:

[0077] S1. Mix 11% of the humectant (2% of butylene glycol, 2% of propylene glycol, 5% of glycerin, and 2% of sorbitol) with 0.01% of the first thickener (xanthan gum) evenly, add it to 49.07% of water, then add 0.1% of the first thickener (carbomer), and finally add 0.08% of the first thickener (triethanolamine) and 0.1% of the first preservative (EDTA disodium) and mix evenly to obtain the A - phase component;

[0078] S2. Mix 6% of the first emulsifier (1% of olive oil unsaponifiables, 1% of sucrose poly stearate, 1% of coco glucoside, 1% of glyceryl stearate, and 2% of PEG - 100 stearate) and 23% of the first emollient (1.5% of stearic acid, 1.5% of shea butter, 5% of ethylhexyl olivate, 2% of jojoba seed oil, 1.5% of cetyl alcohol, 1.5% of cetearyl alcohol, 3% of dimethicone, and 7% of cyclomethicone) evenly to obtain the B - phase component. Then, heat the A - phase component and the B - phase component in a water bath to 68°C respectively, and add the heated B - phase component to the A - phase component for emulsification and homogenization for 5 min; then add 1% of the second thickener (polyacrylamide (and) C13-14 The isoparaffin (and) laureth-7 were homogenized for 1 min; after cooling to ≤45°C, 0.5% of the third thickener (maltodextrin), 5.5% of the soothing agent (2% of magnolia bark extract, 3% of grape seed extract, and 0.5% of dipotassium glycyrrhizate), 0.2% of the second emulsifier (lecithin), 0.4% of the second emollient (0.2% of vitamin E and 0.2% of PEG-40 hydrogenated castor oil), 0.5% of the penetration enhancer (ethanol), 2.5% of the second preservative (0.8% of phenoxyethanol, 0.2% of ethylhexylglycerin, 1% of triethylene glycol, the total amount of imidazolidinyl urea and iodopropynyl butylcarbamate is 0.3%, and 0.2% of p-hydroxyacetophenone), and 0.05% of the fragrance were added in sequence and stirred evenly.

[0079] Example 8

[0080] A preparation method of an antioxidant soothing cream, comprising the following preparation steps:

[0081] S1. Mix 15% of the humectant (3% of butylene glycol, 4% of propylene glycol, 7% of glycerin, and 1% of sorbitol) with 0.05% of the first thickener (xanthan gum) evenly, then add to 46.21% of water, then add 0.1% of the first thickener (carbomer), and finally add 0.08% of the first thickener (triethanolamine) and 0.05% of the first preservative (EDTA disodium) and mix evenly to obtain the A-phase component;

[0082] S2. Mix 3% of the first emulsifier (0.5% of olive oil unsaponifiables, 1% of sucrose poly stearate, 0.5% of coco-glucoside, 0.5% of glyceryl stearate, and 0.5% of PEG-100 stearate) and 25% of the first emollient (1% of stearic acid, 5% of shea butter, 5% of ethylhexyl olivate, 3% of jojoba seed oil, 0.5% of cetyl alcohol, 0.5% of cetearyl alcohol, 2% of polydimethylsiloxane, and 8% of cyclomethicone) evenly to obtain the B-phase component. Then, heat the A-phase component and the B-phase component in a water bath to 70°C respectively, add the heated B-phase component to the A-phase component and emulsify and homogenize for 3 min; then add 1% of the second thickener (polyacrylamide (and) C 13-14Isoparaffin (and) laureth-7) were homogenized for 1 min; after cooling to ≤45°C, 0.5% of the third thickener (maltodextrin), 5% of the soothing agent (3% of magnolia bark extract, 1.5% of grape seed extract, and 0.5% of dipotassium glycyrrhizate, and the concentration ratio of magnolia bark extract to grape seed extract is 1:1), 0.2% of the second emulsifier (lecithin), 1.2% of the second emollient (1% of vitamin E and 0.2% of PEG-40 hydrogenated castor oil), 0.01% of the penetration enhancer (ethanol), 2.55% of the second preservative (0.5% of phenoxyethanol, 0.05% of ethylhexylglycerin, 1.5% of triethylene glycol, the total amount of bis(hydroxymethyl) imidazolidinyl urea and iodopropynyl butylcarbamate is 0.3%, and 0.2% of hydroxyacetophenone), and 0.05% of the fragrance were added and stirred evenly.

[0083] The antioxidant soothing creams of Comparative Example 1 and Examples 5-8 were subjected to the following performance tests:

[0084] 1. Experimental design for reducing redness: This protocol was used to test the reduction of redness on the faces of volunteers. First, microneedle skin punctures were made on the entire face of the volunteers to deliberately create a slight redness reaction, and then the antioxidant soothing creams of Comparative Example 1 and Examples 5-8 were applied. Finally, the skin redness was scored (the scoring basis is as shown in the following table), and the skin redness was evaluated 15 minutes later.

[0085]

[0086]

[0087] From the experimental results in the above table, it can be seen that 15 minutes after applying the antioxidant soothing cream of this protocol, the skin redness significantly decreased, approaching the value before microneedle skin puncture, and the skin heme level returned to the normal level, that is, the antioxidant soothing cream of this protocol has a good immediate soothing effect. In contrast, for the existing antioxidant soothing creams, the corresponding immediate soothing effect after 15 minutes of application is not as good as that of Example 5 of this protocol.

[0088] 2. Experimental design for soothing itching: To further evaluate the effect of the antioxidant soothing cream of this protocol, a test for soothing and anti-itching was carried out on volunteers, and this test was specifically for children's skin because it is not fully developed and is easily affected by the external environment, often showing inflammatory phenomena such as redness and itching. The specific steps are as follows: When redness and itching appear on children's skin, record the severity of the relevant symptoms; then apply the antioxidant soothing cream of Example 1 to the affected area.

[0089] As Figure 6 shown, about 15 minutes after applying the antioxidant soothing cream of Example 1, the itching of the skin improved significantly, the redness area decreased, and the degree of redness also decreased significantly, indicating that this product has an immediate soothing and anti-itching effect.

[0090] 3. Tolerance experiment design: Select 12 volunteers aged 19 - 28 who have skin dryness or sensitivity problems. Expose the inner forearm of these volunteers to ultraviolet light, then determine the MED (Minimum Erythema Dose) value. Then use the antioxidant soothing creams of Comparative Example 1 and Examples 5 - 8 (each group is used by 3 volunteers with the lowest erythema dose), twice a day. After 10 days, determine the MED value again, and record the average value of the MED value of each group.

[0091]

[0092] From the experimental results in the above table, it can be seen that compared with the conventional antioxidant soothing cream, after using the antioxidant soothing creams of Examples 5 - 8 of this scheme, due to its GSP - T + Magnolia composition, it will continuously produce antioxidant effects, can significantly increase the MED value, and improve the skin's tolerance to ultraviolet light.

[0093] 4. Skin improvement effect experiment design: Select 20 volunteers aged 54 - 78. Use the antioxidant soothing creams of Examples 5 - 8 on the face and the inner forearm every day (each group is used by 5 volunteers)

[0094] Twice a day for 28 consecutive days. Then use a skin measuring instrument to test the firmness, water content, and epidermal water loss rate of each part, and record the average value of each group.

[0095] Test items (instruments) and areas:

[0096] ● Epidermal water loss rate (battery flowmeter): face

[0097] ● Water content (skin moisture tester): inner forearm

[0098] ● Firmness (skin elasticity tester): inner forearm

[0099]

[0100] From the experimental results in the above table, it can be seen that after using the antioxidant soothing creams of Examples 5 - 8, the firmness of the skin has doubled compared to the original, the water content has increased by about 30% compared to before use, and the epidermal water loss rate has improved by about three times compared to the original. The antioxidant soothing cream of this scheme has a better effect than the traditional cream. This is mainly because the antioxidant soothing cream can activate the endogenous antioxidant system in the human skin, initiate the antioxidant pathway starting from the cell nucleus, improve the antioxidant effect of each cell, make the cells healthier, and thus improve the overall skin effect.

[0101] The above embodiments are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention. The antioxidant soothing cream mentioned in the present invention is not limited to the above several kinds. Therefore, the above embodiments cannot be regarded as a limitation on the protection scope of the present invention. Any equivalent structural transformation made by using the content of the specification of the present invention under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields, is included in the patent protection scope of the present invention.

Claims

1. An antioxidant composition, characterized in that, It includes the following raw materials: Magnolia officinalis bark extract and grape seed extract.

2. The antioxidant composition according to claim 1, characterized in that, The concentration ratio of the Magnolia officinalis bark extract to the Grape seed extract is 1:1-11.

3. An antioxidant soothing cream, characterized in that, The antioxidant soothing cream comprises the following components, measured by the total weight percentage of the composition: Phase A components: humectant 1-20%, first preservative 0-1.5%, first thickener 0-1% and solvent 30-90%; Phase B components: 1-6% of the first emulsifier and 5-40% of the first emollient; Phase C components: second thickener 0.2-5%; Phase D components: third thickener 0.1-5%, soothing agent 0.5-10%, second emulsifier 0-2%, second emollient 0-1.2%, penetration aid 0-0.5%, second preservative 0-2.55% and fragrance 0-0.05%; The soothing agent comprises the antioxidant composition of claim 1, wherein the extract of Magnolia officinalis bark is 1-5% and the extract of grape seed is 1-5%.

4. An antioxidant soothing cream according to claim 3, characterized in that, The soothing agent in the phase D component also includes 0-0.5% of dipotassium glycyrrhizinate by weight percentage; The third thickener in the phase D component is maltodextrin, the second emulsifier is lecithin, and the penetration enhancer is ethanol; The second emollient in the phase D component includes the following raw materials: vitamin E 0-1.2% and PEG-40 hydrogenated castor oil 0-1.2%; the second preservative in the phase D component includes the following raw materials: phenoxyethanol 0-1.2%, ethylhexylglycerin 0-0.5%, triethylene glycol 0-1.5%, bis(hydroxymethyl)imidazolidinyl urea and iodopropynyl butylcarbamate in total 0-0.3% and parahydroxyacetophenone 0-0.2%.

5. An antioxidant soothing cream according to claim 3, characterized in that, In terms of weight percentage, the moisturizing agent in the phase A component includes the following raw materials: 1-5% butylene glycol, 1-5% propylene glycol, 1-8% glycerol and 1-5% sorbitol.

6. An antioxidant soothing cream according to claim 3 or 5, characterized in that, The first thickener in the phase A component includes the following raw materials by weight percentage: 0-1% hydroxyethyl cellulose, 0.05-0.2% carbomer, 0.01-0.2% xanthan gum and 0.02-0.2% triethanolamine; The first preservative in the phase A component is disodium EDTA, and the solvent is water.

7. An antioxidant soothing cream according to claim 3, characterized in that By weight percentage, the first emulsifier in the phase B component includes the following raw materials: 0.5-5% of olive oil unsaponifiable matter, 1-5% of sucrose polystearate, 0.5-5% of coconut glucoside, 0.5-5% of glyceryl stearate and 0.5-5% of PEG-100 stearate.

8. An antioxidant and soothing cream according to claim 3 or 7, characterized in that By weight percentage, the first emollient in the B phase component includes the following raw materials: 1-5% stearic acid, 1-7% avocado butter, 1-5% hydrogenated ethylhexyl olive oil, 1-6% jojoba seed oil, 0.5-5% cetyl alcohol, 0.5-5% cetearyl alcohol, 1-5% polydimethylsiloxane and 1-15% cyclopolydimethylsiloxane.

9. An antioxidant soothing cream according to claim 3, wherein The second thickener in the C-phase component is polyacrylamide (and) C 13-14 Isoparaffin (and) laureth-7.

10. A method for preparing the antioxidant and soothing cream as described in claim 6, characterized in that, The steps include: S1. The humectant and the xanthan gum or the hydroxyethyl cellulose are mixed evenly and then added to the solvent, and then the carbomer is added, and finally the triethanolamine and the first preservative are added and mixed evenly to obtain a phase A component; S2. After mixing the first emulsifier and the first emollient evenly to obtain the B-phase component, heat the A-phase component and the B-phase component in a water bath to 65-75°C respectively, add the heated B-phase component to the A-phase component and emulsify and homogenize for 1-5 minutes; then add the second thickener and homogenize for 1-5 minutes; after cooling to ≤45°C, add the third thickener, the soothing agent, the second emulsifier, the second emollient, the penetration enhancer, the second preservative and the fragrance agent in sequence and stir evenly.

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