Medicinal and edible functional ointment for preventing and treating senile sarcopenia and preparation method thereof

Abstract

The invention belongs to the field of functional ointment preparation, and particularly relates to medicinal and edible functional ointment for preventing and treating senile sarcopenia and a preparation method of the medicinal and edible functional ointment. Chinese herbal medicine powder obtained through powdering is mixed with whey protein powder, beef extract, pea protein powder and a mold fungicide for fermentation, and muscle synthesis and repair components obtained through adsorption of muscle cell absorption promoting colloids can efficiently promote muscle synthesis and repair; the lactide, the glycolide, the stannous octoate and the dichloromethane are mixed to react to form a polymer, the composition for improving the nerve cell function and the hormone secretion regulating component are mixed, and the nerve cell regulator obtained by spray drying can be endocytosed by small intestine cells; the anti-inflammatory mixture capable of effectively inhibiting inflammatory reaction is mixed with the composition for preventing and treating senile sarcopenia, and the obtained medicinal and edible functional ointment for preventing and treating senile sarcopenia is obvious in senile sarcopenia prevention and treatment effect, high in bioavailability, capable of playing a role for a long time and stable in efficacy in the storage process.

Description

Technical Field

[0001] The present invention belongs to the field of preparation of functional ointments, and specifically refers to a medicated and food homologous functional ointment for preventing and treating sarcopenia in the elderly and its preparation method. Background Art

[0002] Medicated and food homologous preparations refer to preparations that combine food materials with medicinal value and ordinary foods to develop preparations with both nutritional and health care effects. These preparations not only retain the natural nutritional components of the food materials but also exert their medicinal value. They are suitable for the daily health care of special populations such as the elderly. Medicated and food homologous preparations mostly come from natural food materials, usually have high safety and good tolerance, are suitable for long-term use, are relatively gentle on the bodies of the elderly, have small toxic and side effects, and can help the elderly strengthen their physique and prevent various chronic diseases through daily dietary intake.

[0003] Medicated and food homologous preparations are rich in various nutrients, can supplement the nutritional deficiencies caused by insufficient dietary intake in the elderly, and have the effects of immune regulation, improving nutritional anemia, promoting digestion, assisting in reducing blood pressure, blood sugar, blood lipid, improving sleep, antioxidant, and enhancing bone density. When some medicated and food homologous preparations are used in combination with drugs, they can enhance the efficacy of the drugs. Medicated and food homologous preparations are of great significance in the daily health care of the elderly. They can not only meet the nutritional needs but also exert health care effects, and are a safe, effective, and economical health choice.

[0004] At present, the existing preparation technologies of medicaments for preventing and treating sarcopenia in the elderly have the following problems: First, the existing medicaments for preventing and treating sarcopenia in the elderly cannot prevent and treat from multiple aspects, and the curative effect is limited, and the prevention and treatment effect on sarcopenia in the elderly is not obvious; Second, the existing medicaments for preventing and treating sarcopenia in the elderly are easily degraded by the gastrointestinal tract, with low bioavailability and unable to play a long-term role after administration; Third, some active ingredients of the existing medicaments for preventing and treating sarcopenia in the elderly will degrade or inactivate during the preparation and storage processes, affecting the curative effect of the drugs. Summary of the Invention

[0005] In view of the above situation, to overcome the defects of the prior art, the present invention provides a medicated and food homologous functional ointment for preventing and treating sarcopenia in the elderly and its preparation method. To solve the problems that the existing medicaments for preventing and treating sarcopenia in the elderly have an unclear prevention and treatment effect, low bioavailability, inability to play a long-term role, and unstable drug efficacy during storage, the present invention mixes a muscle synthesis and repair component, a nerve cell regulator, and an anti-inflammatory solution. The medicated and food homologous functional ointment for preventing and treating sarcopenia prepared has an obvious effect on preventing and treating sarcopenia in the elderly, high bioavailability, can play a long-term role, and has stable drug efficacy during storage.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows. The present invention provides a functional paste of medicated and edible homologous ingredients for preventing and treating sarcopenia in the elderly. The preparation raw materials of the functional paste of medicated and edible homologous ingredients for preventing and treating sarcopenia in the elderly specifically include the following components in parts by weight: 20 - 23 parts of muscle synthesis and repair components, 15 - 18 parts of nerve cell regulators, 2.5 - 3.5 parts of polygonatum sibiricum extract, 1.8 - 2.3 parts of imperatorin, and 1.6 - 1.9 parts of oxypeucedanin hydrate.

[0007] Preferably, the preparation raw materials of the muscle synthesis and repair components include the following components in parts by weight: 3 - 6 parts of astragalus membranaceus, 2.2 - 2.5 parts of codonopsis pilosula, 2.8 - 3.3 parts of dioscorea opposita, 2.2 - 2.4 parts of poria cocos, 1.6 - 1.9 parts of malt, 4 - 6 parts of whey protein powder, 5 - 8 parts of beef extract, 4.2 - 5.5 parts of pea protein powder, 1 - 1.3 parts of mold agent, 1.8 - 2.1 parts of polyglycolic acid, 1.5 - 1.7 parts of hyaluronic acid, 0.9 - 1.2 parts of lanthanum acetate, and 0.8 - 1.4 parts of sodium caseinate.

[0008] Preferably, the preparation raw materials of the nerve cell regulators include the following components in parts by weight: 2.5 - 3 parts of eucommia ulmoides extract, 1.8 - 2.1 parts of soy isoflavones, 1.3 - 1.6 parts of columbianadin, 1.1 - 1.3 parts of β-hydroxy-β-methylbutyric acid, 0.8 - 1.2 parts of ferulic acid, 0.9 - 1.2 parts of tocotrienol, 1 - 1.2 parts of betaine, 1.5 - 1.8 parts of lactide, 1.5 - 1.8 parts of glycolide, and 0.05 - 0.08 parts of stannous octoate.

[0009] Preferably, the preparation method of the muscle synthesis and repair components specifically includes the following steps: S1. Put astragalus membranaceus, codonopsis pilosula, dioscorea opposita, poria cocos, and malt into a pulverizer with a power of 1.6 - 2.2 kW, and pulverize at a temperature of 25 - 28°C for 20 - 25 min to obtain Chinese herbal medicine powder. S2. Put whey protein powder, beef extract, pea protein powder, the Chinese herbal medicine powder prepared in S1, mold agent, and ultrapure water into a fermentation tank with a power of 9 - 11 kW, ferment at a rotation speed of 300 - 330 r / min and a temperature of 30°C for 3 d. After fermentation, put it into an ultrasonic cell disruptor with a power of 1.2 - 1.5 kW, disrupt at an ultrasonic frequency of 20 kHz and a temperature of 0°C for 10 - 13 min to obtain a promoting repair mixture. S3. Put polyglycolic acid, hyaluronic acid, lanthanum acetate, sodium caseinate, and ultrapure water into a reaction kettle with a power of 2.4 kW, a reaction temperature of 80 °C, a reaction speed of 300 r / min, and a reaction duration of 24 h, and mix them to carry out a cross-linking reaction to obtain a colloid for promoting muscle cell absorption; S4. Put the colloid for promoting muscle cell absorption prepared in S3 and the promoting repair mixture prepared in S2 into a stirring tank with a power of 1.2 - 1.5 kW, a stirring temperature of 25 - 28 °C, a stirring duration of 30 min, and a stirring speed of 110 - 130 r / min, and carry out a mixing adsorption to obtain a muscle synthesis and repair component; In S2, the mold agent is composed of Monascus purpureus, Rhizopus oryzae, Mucor mucedo, and Aspergillus niger, and the weight part mixing ratio is 1.2:1.3:0.9:1.

[0010] Furthermore, in S2, the material-liquid ratio of the mold agent to ultrapure water is 1:100 g / mL. The Monascus purpureus is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC 3.7182. The Rhizopus oryzae is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC 3.5826. The Mucor mucedo is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC 3.5006. The Aspergillus niger is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC 3.13901.

[0011] Furthermore, in S3, the mass fraction of polyglycolic acid in ultrapure water is 5%.

[0012] Preferably, the preparation method of the nerve cell regulator specifically includes the following steps: L1. Put eucommia ulmoides extract, soy isoflavones, and columbianadin into a stirrer with a power of 1.8 kW, a stirring temperature of 28 °C, a stirring duration of 10 - 15 min, and a stirring speed of 300 r / min, and carry out a mixing to obtain a composition for enhancing nerve cell function; L2. Put β-hydroxy-β-methylbutyric acid, ferulic acid, tocotrienol, and betaine into a stirrer with a power of 1.8 kW, a stirring temperature of 28 °C, a stirring duration of 10 - 15 min, and a stirring speed of 300 r / min, and carry out a mixing to obtain a component for regulating hormone secretion; L3. Put lactide, glycolide, stannous octoate, and dichloromethane into a reaction kettle with a power of 2.4 kW, a reaction temperature of 150 °C, a reaction speed of 300 r / min, and a reaction duration of 24 h, and carry out a polymerization reaction to obtain a polymer solution; L4. The polymer solution prepared in L3, the composition for enhancing nerve cell function prepared in L1, and the component for regulating hormone secretion prepared in L2 are put into a stirrer with a power of 1.8 kW. The stirring temperature is 28 °C, the stirring duration is 25 min, and the stirring speed is 500 r / min. After mixing, it is put into a vacuum freeze nano spray dryer with a power of 2.3 - 2.8 kW. The vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40 °C, the particle size of freeze nano spray drying is 100 nm, and the freeze nano spray drying duration is 2.5 - 3 h to obtain a nerve cell regulator.

[0013] Further, in L3, the mass fraction of the lactide in dichloromethane is 8%.

[0014] The present invention also provides a preparation method of a functional paste of medicine and food homology for preventing and treating sarcopenia in the elderly, which specifically includes the following steps: Step 1. Mix the polygonatum sibiricum extract, imperatorin, and oxypeucedanin hydrate, and put them into a stirrer with a power of 1.8 kW. The stirring temperature is 28 °C, the stirring duration is 10 min, and the stirring speed is 300 r / min to obtain an anti-inflammatory mixture. Step 2. Put the muscle synthesis and repair component and the nerve cell regulator into a stirring tank with a power of 2.2 kW. The stirring temperature is 28 °C, the stirring duration is 30 min, and the stirring speed is 300 r / min. After mixing, a composition for preventing and treating sarcopenia in the elderly is obtained. Step 3. Put the anti-inflammatory mixture prepared in Step 1 into the composition for preventing and treating sarcopenia in the elderly prepared in Step 2. The stirring temperature is 28 °C, the stirring duration is 25 - 35 min, and the stirring speed is 500 r / min. After mixing, it is put under an ultraviolet sterilization lamp with a power of 1.8 kW. The ultraviolet sterilization wavelength is 280 nm, the ultraviolet sterilization temperature is 20 °C, and the ultraviolet sterilization duration is 10 min for ultraviolet sterilization to obtain a functional paste of medicine and food homology for preventing and treating sarcopenia in the elderly.

[0015] The beneficial effects achieved by the present invention are as follows: The present invention uses astragalus root, codonopsis pilosula, Chinese yam, poria cocos, and malt, mixes them, and powders them to obtain a Chinese herbal medicine powder that can enhance the digestive and absorption functions of the spleen and stomach, thereby promoting the absorption and utilization of nutrients, providing sufficient raw materials for the synthesis and repair of muscle fibers, improving the body's immunity, accelerating the repair process of muscle fibers by regulating the immune system, mixing whey protein powder, beef extract, pea protein powder with the Chinese herbal medicine powder and mold inoculum for fermentation, decomposing complex proteins into smaller peptides and amino acids that are more easily absorbed, thereby improving the digestive absorption rate of proteins, increasing the content of branched-chain amino acids in whey protein. After ultrasonic disruption, the bioactive peptides contained in the obtained repair-promoting mixture have the effects of antioxidation, anti-inflammation, and promoting muscle synthesis. The metabolites produced during the fermentation process can also improve the balance of the intestinal flora, thereby indirectly promoting muscle recovery and growth. Mix polyglycolic acid, hyaluronic acid, lanthanum acetate, and sodium caseinate. Lanthanum acetate can be used as a cross-linking agent to cross-link with the carboxyl or hydroxyl groups in hyaluronic acid through metal coordination. The molecules of polyglycolic acid, hyaluronic acid, and sodium caseinate combine with each other to form a colloid that can be absorbed by muscle cells with good biocompatibility and adsorb the repair-promoting mixture, which can promote angiogenesis, thereby improving the blood supply of muscle tissue. Sodium caseinate can promote cell adhesion and proliferation, providing a good microenvironment for the growth and repair of muscle cells. The obtained muscle synthesis and repair component can efficiently and continuously promote muscle synthesis and repair; Mix eucommia ulmoides extract, soy isoflavones, and columbianadin to obtain a composition for enhancing nerve cell function, promoting the proliferation and differentiation of neural stem cells, generating more functional neurons, accelerating the recovery of damaged nerves, and improving nerve conduction function and cerebral blood circulation by affecting the synthesis and release of neurotransmitters, providing better nutritional support for nerve cells, enhancing the ability of the brain to send signals to muscles, effectively activating muscle fibers, and promoting muscle contraction and improvement of motor ability. The hormone secretion regulating component can regulate lipid metabolism, increase the secretion of growth hormone and insulin-like growth factor-1, improve the energy supply and energy metabolism of muscle cells, and reduce exercise fatigue. After mixing lactide, glycolide, stannous octoate, and dichloromethane, a ring-opening reaction occurs to form a polymer. The polymer solution is mixed with the composition for enhancing nerve cell function and the hormone secretion regulating component to obtain a nerve cell regulator that can be endocytosed by small intestinal cells, thereby promoting effective cell uptake and absorption, and cooperating with the muscle synthesis and repair component to create a more favorable environment for muscle growth;The polygonatum extract, imperatorin, and oxypeucedanin hydrate are mixed to obtain an anti-inflammatory mixture. The anti-inflammatory mixture can effectively inhibit the inflammatory response by suppressing the release of inflammatory factors, alleviate the symptoms of related inflammatory diseases, scavenge free radicals, reduce the damage of free radicals to cells, thereby creating a good cellular environment for muscle repair. The anti-inflammatory mixture is mixed with a composition for preventing and treating sarcopenia in the elderly to improve the stability of the composition for preventing and treating sarcopenia in the elderly. The obtained functional paste of medicated and edible homology for preventing and treating sarcopenia in the elderly has obvious effects in preventing and treating sarcopenia in the elderly, high bioavailability, can play a long-term role, and has stable efficacy during storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following will be described in a clear and understandable manner in combination with the accompanying drawings. Obviously, the accompanying drawings in the following description are only those of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0017] Figure 1 It is a graph of the myostatin content result described in Experimental Example 2 of the present invention; Figure 2 It is a graph of the irisin content result described in Experimental Example 2 of the present invention; Figure 3 It is a graph of the insulin-like growth factor-1 content result described in Experimental Example 2 of the present invention; Figure 4 It is a graph of the inflammatory factor content result described in Experimental Example 3 of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in combination with the accompanying drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0019] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention. The preferred implementation methods and materials described in the text are only for demonstration purposes, but cannot limit the content of this application.

[0020] The experimental methods in the following embodiments are all conventional methods unless otherwise specified; the test materials and test strains used in the following embodiments are all obtained from commercial channels unless otherwise specified.

[0021] Example 1: This example provides a functional paste of medicated and edible homologous substances for preventing and treating sarcopenia in the elderly and its preparation method. The functional paste of medicated and edible homologous substances for preventing and treating sarcopenia in the elderly contains the following components in parts by weight: 20 parts of muscle synthesis and repair components, 15 parts of nerve cell regulators, 2.5 parts of polygonatum sibiricum extract, 1.8 parts of imperatorin, and 1.6 parts of oxypeucedanin hydrate.

[0022] The preparation raw materials of the muscle synthesis and repair components include the following components in parts by weight: 3 parts of astragalus membranaceus, 2.2 parts of codonopsis pilosula, 2.8 parts of dioscorea opposita, 2.2 parts of poria cocos, 1.6 parts of malt, 4 parts of whey protein powder, 5 parts of beef extract, 4.2 parts of pea protein powder, 1 part of mold agent, 1.8 parts of polyglycolic acid, 1.5 parts of hyaluronic acid, 0.9 part of lanthanum acetate, and 0.8 part of sodium caseinate.

[0023] The preparation raw materials of the nerve cell regulator include the following components in parts by weight: 2.5 parts of eucommia ulmoides extract, 1.8 parts of soy isoflavones, 1.3 parts of columbianadin, 1.1 parts of β-hydroxy-β-methylbutyric acid, 0.8 part of ferulic acid, 0.9 part of tocotrienol, 1 part of betaine, 1.5 parts of lactide, 1.5 parts of glycolide, and 0.05 part of stannous octoate.

[0024] The preparation method of the muscle synthesis and repair components specifically includes the following steps: S1. Put astragalus membranaceus, codonopsis pilosula, dioscorea opposita, poria cocos, and malt into a powder mill with a power of 1.6 kW, a powdering temperature of 25 °C, and a powdering duration of 20 min for powdering to obtain Chinese herbal medicine powder; S2. Put whey protein powder, beef extract, pea protein powder, the Chinese herbal medicine powder prepared in S1, mold agent, and ultrapure water into a fermentation tank with a power of 9 kW, a fermentation rotation speed of 300 r / min, a fermentation temperature of 30 °C, and a fermentation duration of 3 d. After fermentation, put it into an ultrasonic cell disruptor with a power of 1.2 kW, an ultrasonic disruption frequency of 20 kHz, an ultrasonic disruption temperature of 0 °C, and an ultrasonic disruption duration of 10 min. After disruption, obtain a promoting repair mixture; S3. Put polyglycolic acid, hyaluronic acid, lanthanum acetate, sodium caseinate, and ultrapure water into a reaction kettle with a power of 2.4 kW, a reaction temperature of 80 °C, a reaction speed of 300 r / min, and a reaction duration of 24 h for cross-linking reaction to obtain a promoting muscle cell absorption colloid; S4. Put the promoting muscle cell absorption colloid prepared in S3 and the promoting repair mixture prepared in S2 into a stirring tank with a power of 1.2 kW, a stirring temperature of 25 °C, a stirring duration of 30 min, and a stirring rotation speed of 110 r / min for mixing and adsorption to obtain muscle synthesis and repair components; In S2, the mold inoculum is composed of Monascus purpureus, Rhizopus oryzae, Mucor mucedo, and Aspergillus niger, and the weight ratio of mixing is 1.2:1.3:0.9:1.

[0025] In S2, the ratio of the mold inoculum to ultrapure water is 1:100 g / mL. The Monascus purpureus is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.7182. The Rhizopus oryzae is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.5826. The Mucor mucedo is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.5006. The Aspergillus niger is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.13901.

[0026] In S3, the mass fraction of polyglycolic acid in ultrapure water is 5%.

[0027] The preparation method of the nerve cell regulator specifically includes the following steps: L1. Put eucommia ulmoides extract, soy isoflavones, and columbianadin into a stirrer with a power of 1.8 kW, stir at a temperature of 28°C for 10 min, and stir at a speed of 300 r / min for mixing to obtain a composition for enhancing nerve cell function. L2. Put β-hydroxy-β-methylbutyric acid, ferulic acid, tocotrienols, and betaine into a stirrer with a power of 1.8 kW, stir at a temperature of 28°C for 10 min, and stir at a speed of 300 r / min for mixing to obtain a component for regulating hormone secretion. L3. Put lactide, glycolide, stannous octoate, and dichloromethane into a reaction kettle with a power of 2.4 kW, react at a temperature of 150°C, react at a speed of 300 r / min for 24 h to carry out a polymerization reaction to obtain a polymer solution. L4. Put the polymer solution prepared in L3, the composition for enhancing nerve cell function prepared in L1, and the component for regulating hormone secretion prepared in L2 into a stirrer with a power of 1.8 kW, stir at a temperature of 28°C for 25 min, and stir at a speed of 500 r / min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.3 kW, with a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40°C, a freeze nano spray drying particle size of 100 nm, and a freeze nano spray drying duration of 2.5 h to obtain the nerve cell regulator.

[0028] In L3, the mass fraction of lactide in dichloromethane is 8%.

[0029] This embodiment also provides a preparation method of a functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly, which specifically includes the following steps: Step 1: Mix the polygonatum extract, imperatorin, and oxypeucedanin hydrate, put them into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 10 minutes, and stir at a speed of 300 r / min to obtain an anti-inflammatory mixture. Step 2: Put the muscle synthesis and repair component and the nerve cell regulator into a stirring tank with a power of 2.2 kW, stir at a temperature of 28 °C for 30 minutes, and stir at a speed of 300 r / min. After mixing evenly, obtain a composition for preventing and treating sarcopenia in the elderly. Step 3: Put the anti-inflammatory mixture prepared in Step 1 into the composition for preventing and treating sarcopenia prepared in Step 2, stir at a temperature of 28 °C for 25 minutes, and stir at a speed of 500 r / min. After mixing, put it under an ultraviolet sterilization lamp with a power of 1.8 kW, with an ultraviolet sterilization wavelength of 280 nm, an ultraviolet sterilization temperature of 20 °C, and an ultraviolet sterilization duration of 10 minutes for ultraviolet sterilization to obtain a functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly.

[0030] Example 2: This example provides a functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly and its preparation method. The functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly contains the following components in parts by weight: 21 parts of muscle synthesis and repair component, 16 parts of nerve cell regulator, 3 parts of polygonatum extract, 2.1 parts of imperatorin, and 1.7 parts of oxypeucedanin hydrate.

[0031] The preparation raw materials of the muscle synthesis and repair component include the following components in parts by weight: 5 parts of astragalus membranaceus, 2.3 parts of codonopsis pilosula, 3.1 parts of Chinese yam, 2.3 parts of poria cocos, 1.8 parts of malt, 5 parts of whey protein powder, 6 parts of beef extract, 5 parts of pea protein powder, 1.2 parts of mold agent, 1.9 parts of polyglycolic acid, 1.6 parts of hyaluronic acid, 1.1 parts of lanthanum acetate, and 1.2 parts of sodium caseinate.

[0032] The preparation raw materials of the nerve cell regulator include the following components in parts by weight: 2.8 parts of eucommia ulmoides extract, 1.9 parts of soy isoflavones, 1.4 parts of columbianadin, 1.2 parts of β-hydroxy-β-methylbutyric acid, 0.9 part of ferulic acid, 1.1 parts of tocotrienol, 1.1 parts of betaine, 1.6 parts of lactide, 1.6 parts of glycolide, and 0.07 part of stannous octoate.

[0033] The preparation method of the muscle synthesis and repair component specifically includes the following steps: S1. Put astragalus membranaceus, codonopsis pilosula, yam, poria cocos, and malt into a powder mill with a power of 1.9 kW. The powdering temperature is 26 °C, and the powdering duration is 23 min to perform powdering, obtaining Chinese herbal medicine powder. S2. Put whey protein powder, beef extract, pea protein powder, the Chinese herbal medicine powder prepared in S1, mold inoculum, and ultrapure water into a fermenter with a power of 10 kW. The fermentation rotation speed is 310 r / min, the fermentation temperature is 30 °C, and the fermentation duration is 3 d. After fermentation, put it into an ultrasonic cell disruptor with a power of 1.3 kW. The ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0 °C, and the ultrasonic disruption duration is 12 min. After disruption, obtain a promoting repair mixture. S3. Put polyglycolic acid, hyaluronic acid, lanthanum acetate, sodium caseinate, and ultrapure water into a reaction kettle with a power of 2.4 kW. The reaction temperature is 80 °C, the reaction speed is 300 r / min, and the reaction duration is 24 h. Mix them to carry out a cross-linking reaction, obtaining a colloid for promoting muscle cell absorption. S4. Put the colloid for promoting muscle cell absorption prepared in S3 and the promoting repair mixture prepared in S2 into a stirring tank with a power of 1.3 kW. The stirring temperature is 26 °C, the stirring duration is 30 min, and the stirring rotation speed is 120 r / min to carry out mixing and adsorption, obtaining a muscle synthesis and repair component. In S2, the mold inoculum is composed of monascus purpureus, rhizopus oryzae, mucor mucedo, and aspergillus niger, and the weight part mixing ratio is 1.2:1.3:0.9:1.

[0034] In S2, the material liquid ratio of the mold inoculum to ultrapure water is 1:100 g / mL. The monascus purpureus is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.7182. The rhizopus oryzae is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.5826. The mucor mucedo is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.5006. The aspergillus niger is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC 3.13901.

[0035] In S3, the mass fraction of polyglycolic acid in ultrapure water is 5%.

[0036] The preparation method of the nerve cell regulator specifically includes the following steps: L1. Put eucommia ulmoides extract, soy isoflavones, and columbianadin into a stirrer with a power of 1.8 kW. The stirring temperature is 28 °C, the stirring duration is 13 min, and the stirring rotation speed is 300 r / min to carry out mixing, obtaining a composition for enhancing nerve cell function. L2. Put β-hydroxy-β-methylbutyric acid, ferulic acid, tocotrienol, and betaine into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 12 min at a stirring speed of 300 r / min to mix and obtain a component for regulating hormone secretion. L3. Put lactide, glycolide, stannous octoate, and dichloromethane into a reaction kettle with a power of 2.4 kW, react at a temperature of 150 °C at a reaction speed of 300 r / min for 24 h to carry out a polymerization reaction and obtain a polymer solution. L4. Put the polymer solution prepared in L3, the composition for enhancing nerve cell function prepared in L1, and the component for regulating hormone secretion prepared in L2 into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 25 min at a stirring speed of 500 r / min. After mixing evenly, put it into a vacuum freeze-drying nano spray dryer with a power of 2.6 kW, with a vacuum degree of -0.05 MPa, a freeze-drying nano spray drying temperature of -40 °C, a freeze-drying nano spray drying particle size of 100 nm, and a freeze-drying nano spray drying duration of 2.8 h to obtain a nerve cell regulator.

[0037] In L3, the mass fraction of lactide in dichloromethane is 8%.

[0038] This example also provides a preparation method of a homologous functional paste of medicine and food for preventing and treating senile sarcopenia, which specifically includes the following steps: Step 1. Mix polygonatum sibiricum extract, imperatorin, and oxypeucedanin hydrate, put them into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 10 min at a stirring speed of 300 r / min to mix and obtain an anti-inflammatory mixture. Step 2. Put the muscle synthesis and repair component and the nerve cell regulator into a stirring tank with a power of 2.2 kW, stir at a temperature of 28 °C for 30 min at a stirring speed of 300 r / min. After mixing evenly, obtain a composition for preventing and treating senile sarcopenia. Step 3. Put the anti-inflammatory mixture prepared in Step 1 into the composition for preventing and treating senile sarcopenia prepared in Step 2, stir at a temperature of 28 °C for 30 min at a stirring speed of 500 r / min. After mixing, put it under an ultraviolet sterilization lamp with a power of 1.8 kW, with an ultraviolet sterilization wavelength of 280 nm, an ultraviolet sterilization temperature of 20 °C, and an ultraviolet sterilization duration of 10 min to carry out ultraviolet sterilization and obtain a homologous functional paste of medicine and food for preventing and treating senile sarcopenia.

[0039] Example 3: This example provides a functional paste of medicated diet for preventing and treating sarcopenia in the elderly and its preparation method. The functional paste of medicated diet for preventing and treating sarcopenia in the elderly contains the following components in parts by weight: 23 parts of muscle synthesis and repair components, 18 parts of nerve cell regulators, 3.5 parts of polygonatum sibiricum extract, 2.3 parts of imperatorin, and 1.9 parts of oxypeucedanin hydrate.

[0040] The preparation raw materials of the muscle synthesis and repair components include the following components in parts by weight: 6 parts of astragalus membranaceus, 2.5 parts of codonopsis pilosula, 3.3 parts of dioscorea opposita, 2.4 parts of poria cocos, 1.9 parts of malt, 6 parts of whey protein powder, 8 parts of beef extract, 5.5 parts of pea protein powder, 1.3 parts of mold agent, 2.1 parts of polyglycolic acid, 1.7 parts of hyaluronic acid, 1.2 parts of lanthanum acetate, and 1.4 parts of sodium caseinate.

[0041] The preparation raw materials of the nerve cell regulators include the following components in parts by weight: 3 parts of eucommia ulmoides extract, 2.1 parts of soy isoflavones, 1.6 parts of columbianadin, 1.3 parts of β-hydroxy-β-methylbutyric acid, 1.2 parts of ferulic acid, 1.2 parts of tocotrienol, 1.2 parts of betaine, 1.8 parts of lactide, 1.8 parts of glycolide, and 0.08 parts of stannous octoate.

[0042] The preparation method of the muscle synthesis and repair components specifically includes the following steps: S1. Put astragalus membranaceus, codonopsis pilosula, dioscorea opposita, poria cocos, and malt into a pulverizer with a power of 2.2 kW, the pulverizing temperature is 28 °C, and the pulverizing time is 25 min to carry out pulverization to obtain Chinese herbal medicine powder; S2. Put whey protein powder, beef extract, pea protein powder, the Chinese herbal medicine powder prepared in S1, mold agent, and ultrapure water into a fermentation tank with a power of 11 kW, the fermentation rotation speed is 330 r / min, the fermentation temperature is 30 °C, and the fermentation time is 3 d. After fermentation, put it into an ultrasonic cell disruptor with a power of 1.5 kW, the ultrasonic disruption frequency is 20 kHz, the ultrasonic disruption temperature is 0 °C, and the ultrasonic disruption time is 13 min. After disruption, obtain a promoting repair mixture; S3. Put polyglycolic acid, hyaluronic acid, lanthanum acetate, sodium caseinate, and ultrapure water into a reaction kettle with a power of 2.4 kW, the reaction temperature is 80 °C, the reaction speed is 300 r / min, and the reaction time is 24 h. Mix and carry out a cross-linking reaction to obtain a colloid for promoting muscle cell absorption; S4. Put the colloid for promoting muscle cell absorption prepared in S3 and the promoting repair mixture prepared in S2 into a stirring tank with a power of 1.5 kW, the stirring temperature is 28 °C, the stirring time is 30 min, and the stirring rotation speed is 130 r / min to carry out mixing and adsorption to obtain muscle synthesis and repair components; In S2, the mold inoculum is composed of Monascus purpureus, Rhizopus oryzae, Mucor mucedo, and Aspergillus niger, and the mixing ratio by weight is 1.2:1.3:0.9:1.

[0043] In S2, the ratio of the mold inoculum to ultrapure water is 1:100 g / mL. The Monascus purpureus is purchased from the China General Microbiological Culture Collection Center with the preservation number CGMCC 3.7182. The Rhizopus oryzae is purchased from the China General Microbiological Culture Collection Center with the preservation number CGMCC 3.5826. The Mucor mucedo is purchased from the China General Microbiological Culture Collection Center with the preservation number CGMCC 3.5006. The Aspergillus niger is purchased from the China General Microbiological Culture Collection Center with the preservation number CGMCC 3.13901.

[0044] In S3, the mass fraction of polyglycolic acid in ultrapure water is 5%.

[0045] The preparation method of the nerve cell regulator specifically includes the following steps: L1. Put eucommia ulmoides extract, soy isoflavones, and columbianadin into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 15 min, and at a rotation speed of 300 r / min for mixing to obtain a composition for enhancing nerve cell function; L2. Put β-hydroxy-β-methylbutyric acid, ferulic acid, tocotrienols, and betaine into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 15 min, and at a rotation speed of 300 r / min for mixing to obtain a component for regulating hormone secretion; L3. Put lactide, glycolide, stannous octoate, and dichloromethane into a reaction kettle with a power of 2.4 kW, react at a temperature of 150 °C, at a reaction speed of 300 r / min for 24 h to carry out a polymerization reaction to obtain a polymer solution; L4. Put the polymer solution prepared in L3, the composition for enhancing nerve cell function prepared in L1, and the component for regulating hormone secretion prepared in L2 into a stirrer with a power of 1.8 kW, stir at a temperature of 28 °C for 25 min, and at a rotation speed of 500 r / min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.8 kW, with a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40 °C, a freeze nano spray drying particle size of 100 nm, and a freeze nano spray drying duration of 3 h to obtain the nerve cell regulator.

[0046] In L3, the mass fraction of lactide in dichloromethane is 8%.

[0047] This embodiment also provides a preparation method of a functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly, which specifically includes the following steps: Step 1: Mix the polygonatum extract, imperatorin, and oxypeucedanin hydrate, put them into a stirrer with a power of 1.8 kW, stir at a temperature of 28°C for 10 minutes, and stir at a speed of 300 r / min to obtain an anti-inflammatory mixture. Step 2: Put the muscle synthesis and repair components and the nerve cell regulator into a stirring tank with a power of 2.2 kW, stir at a temperature of 28°C for 30 minutes, and stir at a speed of 300 r / min. After mixing evenly, obtain a composition for preventing and treating sarcopenia in the elderly. Step 3: Put the anti-inflammatory mixture prepared in Step 1 into the composition for preventing and treating sarcopenia in the elderly prepared in Step 2, stir at a temperature of 28°C for 35 minutes, and stir at a speed of 500 r / min. After mixing, put it under a UV sterilization lamp with a power of 1.8 kW, with a UV sterilization wavelength of 280 nm, a UV sterilization temperature of 20°C, and a UV sterilization time of 10 minutes for UV sterilization to obtain a functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly.

[0048] Comparative Example 1: This comparative example provides a paste for preventing and treating sarcopenia in the elderly and its preparation method. The difference from Example 1 is only that the added muscle synthesis and repair components do not contain the colloid promoting muscle cell absorption, and the other components, component contents, and method steps are the same as those in Example 1.

[0049] Comparative Example 2: This comparative example provides a paste for preventing and treating sarcopenia in the elderly and its preparation method. The difference from Example 1 is only that the added nerve cell regulator does not contain the polymer solution, and the other components, component contents, and method steps are the same as those in Example 1.

[0050] Experimental Example 1: Determination test of the efficacy of preventing and treating sarcopenia.

[0051] For the functional paste with both medicinal and edible properties for preventing and treating sarcopenia in the elderly prepared in Examples 1-3 of the present invention, the steps of the prevention and treatment efficacy determination test are as follows: (1) Select 30 elderly patients with sarcopenia, divide them into 6 groups on average, with 5 patients in each group. (2)The elderly sarcopenia patients in the Example 1-3 groups were on a regular diet and took the functional paste of medicated diet for preventing and treating elderly sarcopenia prepared by the Example 1-3 groups, 2 g each time, twice a day. The elderly sarcopenia patients in the Comparative Example 1-2 groups were on a regular diet and took the paste for preventing and treating elderly sarcopenia prepared by the Comparative Example 1-2 groups, 2 g each time, twice a day. The elderly sarcopenia patients in the control group were on a regular diet and took the commercially available nutritional supplement for preventing and treating elderly sarcopenia (purchased from Abbott Laboratories Limited), 2 g each time, twice a day. Each group took the medicine continuously for 4 weeks. (3)After taking the medicine continuously for 4 weeks, the walking speed (walking straight for 4 m), grip strength, calf circumference, and body mass index (BMI) of the elderly sarcopenia patients in the Example 1-3 groups, Comparative Example 1-2 groups, and control group were observed before and after taking the medicine and recorded in Table 1.

[0052] Table 1. Changes in anthropometric parameters before and after taking the medicine

[0053] Result analysis: As shown in Table 1, the elderly sarcopenia patients in the Example 1-3 groups took the functional paste of medicated diet for preventing and treating elderly sarcopenia prepared by the Example 1-3 groups, 2 g each time, twice a day, continuously for 4 weeks. The walking speed (walking straight for 4 m), grip strength, calf circumference, and body mass index were significantly improved, which was significantly better than the prevention and treatment effects of the control group and the Comparative Example 1 group on elderly sarcopenia. It shows that the functional paste of medicated diet for preventing and treating elderly sarcopenia prepared by the present invention has obvious prevention and treatment effects on elderly sarcopenia and high bioavailability.

[0054] Experimental Example 2: Determination test of prevention and treatment effect after storage.

[0055] The steps of the determination test of the prevention and treatment effect after storage of the functional paste of medicated diet for preventing and treating elderly sarcopenia prepared in the Example 1-3 of the present invention are as follows: (1)Sixty clean-grade 6-week-old male C57BL / 6 healthy mice, each weighing 25 g, were housed separately in cages, with free access to food and water. The light cycle was 12 h of light and 12 h of darkness. They were randomly divided into 6 groups of 10 mice each. By gavage, the mice were given buprenorphine at a dose of 0.05 mg / kg. The mice were anesthetized with isoflurane, and a sterile 1.2% BaCl2 solution was injected into the gastrocnemius muscle of the mice at a dose of 3.2 mL / kg. The mice were then returned to their cages until they could move. Six hours later, the mice were given buprenorphine at a dose of 0.05 mg / kg again. After 2 h of injury, muscle injury model mice were obtained. By gavage, the mice in the experimental groups 1-3 were given 0.25 g of the functional food and drug homologous paste for preventing and treating senile sarcopenia prepared in experimental groups 1-3 every day, the mice in the control groups 1-2 were given 0.25 g of the paste for preventing and treating senile sarcopenia prepared in control groups 1-2 every day, and the mice in the control group were given 0.25 g of a commercially available nutritional supplement for preventing and treating senile sarcopenia (purchased from Abbott Laboratories) every day for 12 consecutive days; (2)After 12 d, the mice were sacrificed by cervical dislocation. The intact gastrocnemius muscle tissues of each mouse were taken out, rinsed with 0.01 mol / L PBS buffer at 0°C with a pH of 7.4 to remove residual blood, weighed, and then cut into small pieces. The cut tissues were added to a glass homogenizer with 0.01 mol / L PBS buffer at a pH of 7.4 at a volume ratio of 1:10, and ground thoroughly on ice. The homogenate was ultrasonically broken using an ultrasonic crusher to further lyse the tissue cells. The lysed tissue cell solution was transferred to a 2 mL sterile centrifuge tube and centrifuged at a speed of 5000 r / min for 10 min in a high-speed centrifuge to obtain the supernatant of the mouse gastrocnemius muscle tissue; (3)Using a myostatin (MSTN) kit (Ailianda, product number: ALD38893), an irisin ELISA kit (Enzyme-linked Biotechnology, product number: ml105988), and an insulin-like growth factor-1 (IGF–1) ELISA kit (Enzyme-linked Biotechnology, product number: ml202807), the contents of myostatin, irisin, and insulin-like growth factor-1 in the supernatant of the mouse gastrocnemius muscle tissue were measured using an automatic biochemical analyzer and recorded; (4)Under the conditions of a temperature of 28°C and a humidity of 60%, the functional food and drug homologous paste for preventing and treating senile sarcopenia prepared in experimental groups 1-3, the paste for preventing and treating senile sarcopenia prepared in control groups 1-2, and the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group were stored for 48 d to obtain the stored functional food and drug homologous paste for preventing and treating senile sarcopenia prepared in experimental groups 1-3, the stored paste for preventing and treating senile sarcopenia prepared in control groups 1-2, and the stored commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group; (5)Sixty clean-grade 6-week-old male C57BL / 6 healthy mice, each weighing 25 g, were housed separately in cages, fed and watered ad libitum, with a light cycle of alternating day and night, 12 h:12 h. They were randomly divided into 6 groups of 10 mice each. By gavage, the mice were given 0.05 mg / kg of buprenorphine. The mice were anesthetized with isoflurane, and a sterile 1.2% BaCl2 solution was injected into the gastrocnemius muscle of the mice at a dose of 3.2 mL / kg. The mice were then returned to their cages until they could move. Six hours later, the mice were given 0.05 mg / kg of buprenorphine again. After 2 h of injury, muscle injury model mice were obtained. By gavage, the mice in the experimental groups 1-3 were given 0.25 g of the functional food and medicine homologous paste for preventing and treating senile sarcopenia prepared in Examples 1-3 after storage every day, the mice in the control groups 1-2 were given 0.25 g of the paste for preventing and treating senile sarcopenia prepared in the control groups 1-2 after storage every day, and the mice in the control group were given 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group after storage every day. They were fed continuously for 12 d, and steps (2)-(3) were repeated for result recording.

[0056] Result analysis: Figure 1This is the result graph of the myostatin content described in Experimental Example 2 of the present invention. As shown in the figure, the muscle-injured mice in the Experimental Example 1-3 groups were respectively administered 0.25 g of the homologous functional paste of medicine and food for preventing and treating senile sarcopenia prepared in the Experimental Example 1-3 groups every day. After continuous feeding for 12 days, the myostatin contents in the gastrocnemius muscles of the mice were 420.30 pg / mL, 416.43 pg / mL, and 413.39 pg / mL respectively. The muscle-injured mice in the Experimental Example 1-3 groups were respectively administered 0.25 g of the homologous functional paste of medicine and food for preventing and treating senile sarcopenia prepared in the Experimental Example 1-3 groups after storage every day. After continuous feeding for 12 days, the myostatin contents in the gastrocnemius muscles of the mice were 421.38 pg / mL, 417.39 pg / mL, and 414.52 pg / mL respectively. The muscle-injured mice in the Comparative Example 1-2 groups were respectively administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in the Comparative Example 1-2 groups every day. After continuous feeding for 12 days, the myostatin contents in the gastrocnemius muscles of the mice were 425.51 pg / mL and 428.25 pg / mL respectively. The muscle-injured mice in the Comparative Example 1-2 groups were respectively administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in the Comparative Example 1-2 groups after storage every day. After continuous feeding for 12 days, the myostatin contents in the gastrocnemius muscles of the mice were 427.69 pg / mL and 429.71 pg / mL respectively. The muscle-injured mice in the control group were administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group every day. After continuous feeding for 12 days, the myostatin content in the gastrocnemius muscle of the mice was 443.58 pg / mL. The muscle-injured mice in the control group were administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group after storage every day. After continuous feeding for 12 days, the myostatin content in the gastrocnemius muscle of the mice was 466.57 pg / mL; Figure 2This is the result graph of irisin content described in Experimental Example 2 of the present invention. As shown in the figure, the muscle injury model mice in the first to third groups of Examples were respectively administered 0.25 g of the medicated and edible homologous functional paste for preventing and treating senile sarcopenia prepared in the first to third groups of Examples every day. After continuous feeding for 12 days, the irisin contents in the gastrocnemius muscles of the mice were 321.55 pg / mL, 325.45 pg / mL, and 328.52 pg / mL respectively. The muscle injury model mice in the first to third groups of Examples were respectively administered 0.25 g of the medicated and edible homologous functional paste for preventing and treating senile sarcopenia prepared in the first to third groups of Examples after storage every day. After continuous feeding for 12 days, the irisin contents in the gastrocnemius muscles of the mice were 320.18 pg / mL, 324.65 pg / mL, and 327.29 pg / mL respectively. The muscle injury model mice in the first and second groups of Comparative Examples were respectively administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in the first and second groups of Comparative Examples every day. After continuous feeding for 12 days, the irisin contents in the gastrocnemius muscles of the mice were 318.42 pg / mL and 314.54 pg / mL respectively. The muscle injury model mice in the first and second groups of Comparative Examples were respectively administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in the first and second groups of Comparative Examples after storage every day. After continuous feeding for 12 days, the irisin contents in the gastrocnemius muscles of the mice were 315.24 pg / mL and 310.71 pg / mL respectively. The muscle injury model mice in the control group were administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group every day. After continuous feeding for 12 days, the irisin content in the gastrocnemius muscle of the mice was 280.51 pg / mL. The muscle injury model mice in the control group were administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group after storage every day. After continuous feeding for 12 days, the irisin content in the gastrocnemius muscle of the mice was 264.74 pg / mL; Figure 3This is the result graph of the insulin-like growth factor-1 content described in Experimental Example 2 of the present invention. As shown in the figure, the muscle injury model mice in Example 1-3 groups were respectively administered 0.25 g of the medicated and edible homologous functional paste for preventing and treating senile sarcopenia prepared in Example 1-3 groups every day. After continuous feeding for 12 days, the insulin-like growth factor-1 contents in the gastrocnemius muscles of the mice were 283.55 pg / mL, 288.51 pg / mL, and 292.37 pg / mL respectively. The muscle injury model mice in Example 1-3 groups were respectively administered 0.25 g of the medicated and edible homologous functional paste for preventing and treating senile sarcopenia prepared in Example 1-3 groups after storage every day. After continuous feeding for 12 days, the insulin-like growth factor-1 contents in the gastrocnemius muscles of the mice were 282.29 pg / mL, 287.34 pg / mL, and 291.25 pg / mL respectively. The muscle injury model mice in Comparative Example 1-2 groups were respectively administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in Comparative Example 1-2 groups every day. After continuous feeding for 12 days, the insulin-like growth factor-1 contents in the gastrocnemius muscles of the mice were 276.49 pg / mL and 272.36 pg / mL respectively. The muscle injury model mice in Comparative Example 1-2 groups were respectively administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in Comparative Example 1-2 groups after storage every day. After continuous feeding for 12 days, the insulin-like growth factor-1 contents in the gastrocnemius muscles of the mice were 273.45 pg / mL and 265.42 pg / mL respectively. The muscle injury model mice in the control group were administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group every day. After continuous feeding for 12 days, the insulin-like growth factor-1 content in the gastrocnemius muscle of the mice was 264.32 pg / mL. The muscle injury model mice in the control group were administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group after storage every day. After continuous feeding for 12 days, the insulin-like growth factor-1 content in the gastrocnemius muscle of the mice was 241.71 pg / mL. It shows that the medicated and edible homologous functional paste for preventing and treating senile sarcopenia prepared by the present invention can effectively prevent and treat senile sarcopenia, and the drug effect is stable during storage.

[0057] Experimental Example 3: Inflammatory level measurement test.

[0058] For the medicated and edible homologous functional paste for preventing and treating senile sarcopenia prepared in Example 1-3 of the present invention, the steps for the measurement test of the long-term effect on the inflammatory level are as follows: (1) Sixty clean-grade 6-week-old male C57BL / 6 healthy mice, each weighing 25 g, were selected and housed separately in cages, with free access to food and water. The light cycle was 12 h of light and 12 h of darkness in alternation. They were randomly divided into 6 groups of 10 mice each. By gavage, the mice were given 0.05 mg / kg of buprenorphine. The mice were anesthetized with isoflurane, and a sterile 1.2% BaCl2 solution was injected into the gastrocnemius muscle of the mice at a dose of 3.2 mL / kg. The mice were then returned to their cages until they could move. Six hours later, the mice were given 0.05 mg / kg of buprenorphine again. After 2 h of injury, muscle injury model mice were obtained. By gavage, the mice in Example 1-3 groups were given 0.25 g of the functional food and medicine homologous paste for preventing and treating senile sarcopenia prepared in Example 1-3 groups respectively every day, the mice in Comparative Example 1-2 groups were given 0.25 g of the paste for preventing and treating senile sarcopenia prepared in Comparative Example 1-2 groups respectively every day, and the control group mice were given 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia (purchased from Abbott Laboratories) every day. After continuous feeding for 12 d, the feeding was stopped; (2) Twenty days after stopping feeding, the mice were sacrificed by cervical dislocation. The intact gastrocnemius muscle tissues of each mouse were taken out, rinsed with 0.01 mol / L PBS buffer solution at 0°C with a pH of 7.4 to remove residual blood, weighed, and then the tissues were minced. The minced tissues and 0.01 mol / L PBS buffer solution with a pH of 7.4 were added to a glass homogenizer at a volume ratio of 1:10 and ground thoroughly on ice. The homogenate was ultrasonically broken using an ultrasonic crusher to further lyse the tissue cells. The lysed tissue cell solution was transferred to a 2 mL sterile centrifuge tube and centrifuged at a speed of 5000 r / min for 10 min in a high-speed centrifuge to obtain the supernatant of the mouse gastrocnemius muscle tissue; (3) Mouse interleukin-6 (IL-6) ELISA kit (Saipai Bio, product number: SP13755), mouse interleukin-1β (IL-1β) kit (Enzyme-linked Bio, product number: ml098416), and mouse tumor necrosis factor-α (TNF-α) kit (Enzyme-linked Bio, product number: ml002095) were used. The contents of interleukin-6, interleukin-1β, and tumor necrosis factor-α in the supernatant of the mouse gastrocnemius muscle tissue were measured using an automatic biochemical analyzer and recorded.

[0059] Result analysis: Figure 4This is the result graph of the inflammatory factor content described in Experimental Example 3 of the present invention. As shown in the figure, the muscle injury model mice in Example 1-3 groups were orally administered 0.25 g of the functional paste of food and medicine homology for preventing and treating senile sarcopenia prepared in Example 1-3 groups every day. After continuous feeding for 12 d, the feeding was stopped. After 20 d of stopping feeding, the interleukin 6 contents in the gastrocnemius muscles of the mice were 22.51 pg / mL, 20.27 pg / mL, and 17.28 pg / mL respectively, the interleukin-1β contents in the gastrocnemius muscles of the mice were 45.35 pg / mL, 42.26 pg / mL, and 38.31 pg / mL respectively, and the tumor necrosis factor-α contents in the gastrocnemius muscles of the mice were 436.43 pg / mL, 418.38 pg / mL, and 407.44 pg / mL respectively. The muscle injury model mice in Comparative Example 1-2 groups were orally administered 0.25 g of the paste for preventing and treating senile sarcopenia prepared in Comparative Example 1-2 groups every day. After continuous feeding for 12 d, the feeding was stopped. After 20 d of stopping feeding, the interleukin 6 contents in the gastrocnemius muscles of the mice were 25.59 pg / mL and 29.49 pg / mL respectively, the interleukin-1β contents in the gastrocnemius muscles of the mice were 48.33 pg / mL and 50.45 pg / mL respectively, and the tumor necrosis factor-α contents in the gastrocnemius muscles of the mice were 440.37 pg / mL and 444.28 pg / mL respectively. The muscle injury model mice in the control group were orally administered 0.25 g of the commercially available nutritional supplement for preventing and treating senile sarcopenia in the control group every day. After continuous feeding for 12 d, the feeding was stopped. After 20 d of stopping feeding, the interleukin 6 content in the gastrocnemius muscle of the mice was 33.62 pg / mL, the interleukin-1β content in the gastrocnemius muscle of the mice was 57.33 pg / mL, and the tumor necrosis factor-α content in the gastrocnemius muscle of the mice was 478.36 pg / mL. It shows that the functional paste of food and medicine homology for preventing and treating senile sarcopenia prepared by the present invention has a significant anti-inflammatory effect and can play a long-term role.

[0060] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made therein without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

[0061] The present invention and its embodiments have been described above. Such description is not restrictive. What is shown in the drawings is only one of the embodiments of the present invention, and the actual application is not limited thereto. Generally speaking, if those of ordinary skill in the art are inspired by it and design similar ways and embodiments without creative efforts without departing from the purpose of the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A functional paste of medicine and food homology for preventing and treating sarcopenia in the elderly, characterized in that, The medicated and edible homologous functional paste for preventing and treating sarcopenia in the elderly specifically comprises the following components in parts by weight: 20 - 23 parts of muscle synthesis and repair components, 15 - 18 parts of nerve cell regulators, 2.5 - 3.5 parts of polygonatum sibiricum extract, 1.8 - 2.3 parts of imperatorin, 1.6 - 1.9 parts of oxypeucedanin hydrate; The preparation raw materials of the muscle synthesis and repair components include the following components in parts by weight: 3 - 6 parts of astragalus membranaceus, 2.2 - 2.5 parts of codonopsis pilosula, 2.8 - 3.3 parts of dioscorea opposita, 2.2 - 2.4 parts of poria cocos, 1.6 - 1.9 parts of malt, 4 - 6 parts of whey protein powder, 5 - 8 parts of beef extract, 4.2 - 5.5 parts of pea protein powder, 1 - 1.3 parts of mold agent, 1.8 - 2.1 parts of polyglycolic acid, 1.5 - 1.7 parts of hyaluronic acid, 0.9 - 1.2 parts of lanthanum acetate, 0.8 - 1.4 parts of sodium caseinate; The preparation raw materials of the nerve cell regulators include the following components in parts by weight: 2.5 - 3 parts of eucommia ulmoides extract, 1.8 - 2.1 parts of soy isoflavones, 1.3 - 1.6 parts of columbianadin, 1.1 - 1.3 parts of β-hydroxy-β-methylbutyric acid, 0.8 - 1.2 parts of ferulic acid, 0.9 - 1.2 parts of tocotrienol, 1 - 1.2 parts of betaine, 1.5 - 1.8 parts of lactide, 1.5 - 1.8 parts of glycolide, 0.05 - 0.08 parts of stannous octoate.

2. The homologous medicine and food functional plaster for preventing and treating sarcopenia in the elderly according to claim 1, characterized in that, The preparation method of the muscle synthesis and repair components specifically comprises the following steps: S1. Powder astragalus membranaceus, codonopsis pilosula, dioscorea opposita, poria cocos, and malt to obtain Chinese herbal medicine powder; S2. Mix whey protein powder, beef extract, pea protein powder, the Chinese herbal medicine powder prepared in S1, mold agent, and ultrapure water, ferment, and then perform ultrasonic crushing to obtain a promoting repair mixture; S3. Mix polyglycolic acid, hyaluronic acid, lanthanum acetate, sodium caseinate, and ultrapure water to react to obtain a colloid for promoting muscle cell absorption; S4. Stir and adsorb the colloid for promoting muscle cell absorption prepared in S3 and the promoting repair mixture prepared in S2 to obtain muscle synthesis and repair components; In S2, the mold agent consists of monascus purpureus, rhizopus oryzae, mucor mucedo, and aspergillus niger, and the mixing ratio in parts by weight is 1.2:1.3:0.9:

1.

3. The homologous medicine and food functional paste for preventing and treating sarcopenia in the elderly according to claim 2, wherein The preparation method of the nerve cell regulators specifically comprises the following steps: L1. Mix eucommia ulmoides extract, soy isoflavones, and columbianadin to obtain a composition for enhancing nerve cell function; L2. Mix β-hydroxy-β-methylbutyric acid, ferulic acid, tocotrienol, and betaine to obtain a component for regulating hormone secretion; L3. Mix lactide, glycolide, stannous octoate, and dichloromethane to react to obtain a polymer solution; L4. Mix the polymer solution prepared in L3, the composition for enhancing nerve cell function prepared in L1, and the component for regulating hormone secretion prepared in L2, and after mixing evenly, perform freeze-drying by nano-spraying to obtain nerve cell regulators.

4. A preparation method of a medicine-food homologous functional paste for preventing and treating sarcopenia in the elderly according to any one of claims 1-3, characterized in that, Specifically, it comprises the following steps: Step 1. Mix polygonatum sibiricum extract, imperatorin, and oxypeucedanin hydrate to obtain an anti-inflammatory mixture; Step 2. Mix the muscle synthesis and repair components and the nerve cell regulators evenly to obtain a composition for preventing and treating sarcopenia in the elderly; Step 3: Put the anti-inflammatory mixture prepared in Step 1 into the composition for preventing and treating sarcopenia in the elderly prepared in Step 2. After mixing, perform ultraviolet sterilization to obtain a functional paste of medicine and food homology for preventing and treating sarcopenia in the elderly.

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