A detection device for detecting infectious pathogens using a microfluidic chip

Abstract

The present invention discloses a detection device for detecting infectious pathogens using a microfluidic chip, and relates to the field of detection technology. The present invention provides a disinfection chamber on one side of the detection chamber, and provides a through groove to connect the detection chamber and the disinfection chamber. The microfluidic chip is moved laterally by cooperating with a traction mechanism, so that the microfluidic chip can move back and forth between the detection chamber and the disinfection chamber. Before the infectious pathogen sample is tested, the microfluidic chip can be cleaned and disinfected in advance and directly loaded into the detection chamber without contact with the outside world. This can avoid the impact of contamination of the microfluidic chip during manual loading on the detection result, which is conducive to ensuring the accuracy of the detection. After the detection is completed, the microfluidic chip can be automatically transferred to the disinfection chamber for disinfection and cleaning, which can effectively reduce the probability of the operator contacting the used microfluidic chip, and is conducive to ensuring the safety of the detection.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a detection device for detecting infectious pathogens using a microfluidic chip. Background Art

[0002] In the field of medical testing, microfluidic chip detection technology has been widely used in the detection of infectious pathogens due to its advantages such as miniaturization, integration, fast analysis speed, and small amount of samples and reagents required.

[0003] At present, when microfluidic chip-based detection devices are actually used, operators are usually required to manually load the microfluidic chip into the detection equipment. This manual loading process has obvious defects. During the loading process, since the operator needs to have direct contact with the microfluidic chip, the microfluidic chip is easily contaminated. Once the microfluidic chip is contaminated, foreign contaminants may interfere with the biochemical reactions in the subsequent detection process, thereby causing deviations in the test results and false positive or false negative results, which will affect the accuracy of the infectious pathogen detection results.

[0004] To this end, a detection device for detecting infectious pathogens using a microfluidic chip is proposed to solve some of the problems existing in the above-mentioned prior art. Summary of the Invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art in the process of infectious pathogen detection, in which the disinfected and cleaned microfluidic chip is installed in the detection device by manual loading, which may easily cause contamination and affect the detection accuracy due to careless operation. A detection device for detecting infectious pathogens using a microfluidic chip is proposed.

[0006] In order to solve the problems existing in the prior art, the present invention adopts the following technical solutions:

[0007] A detection device for detecting infectious pathogens using a microfluidic chip includes a main body, a detection chamber is provided in the main body, and a first cover plate is rotatably mounted on the detection chamber, a loading slot is fixed in the detection chamber, and a joint is fixed on the loading slot, a disinfection chamber arranged side by side with the detection chamber is provided in the main body, and a second cover plate is rotatably mounted on the disinfection chamber, a card rack arranged corresponding to the loading slot is installed in the disinfection chamber, a liquid supply pipe and a plurality of first nozzles connected to the liquid supply pipe are fixed on the second cover plate, a drain port is provided at the bottom of the disinfection chamber, an air pump is fixed in the main body, an air supply pipe and a plurality of second nozzles connected to the air supply pipe are fixed on the second cover plate, an electric heating component is provided in the air supply pipe, an exhaust pipe is connected to the disinfection chamber, a through groove is provided in the main body connecting between the detection chamber and the disinfection chamber, the through groove and the card rack are in the same plane and are located above the loading slot, a sealing plate is vertically slidably mounted in the through groove, a first electric push rod for driving the sealing plate to rise and fall is fixed in the main body, and a traction mechanism is provided on the side of the detection chamber away from the disinfection chamber.

[0008] Preferably, a second nozzle is plugged into a first nozzle, the second nozzle is coaxially arranged with the corresponding first nozzle, and the second nozzle is hidden inside the corresponding first nozzle.

[0009] Preferably, a rubber bag is fixed on one side of the sealing plate close to the sterilization chamber, and the rubber bag is filled with argon gas.

[0010] Preferably, the traction mechanism includes a connecting block housed in the end wall of the detection cavity, and the connecting block and the through slot are located in the same plane. A second electric push rod is fixed in the main body for driving the connecting block to move horizontally, and a first air suction hole is opened on the side of the connecting block close to the detection cavity.

[0011] Preferably, a lifting platform arranged below the middle position of the loading slot is installed in the detection chamber for lifting, and a second air suction hole is opened on the top of the lifting platform.

[0012] Preferably, a first piston chamber located below the lifting platform is provided in the main body, a first column rod vertically inserted in the first piston chamber is fixed to the bottom of the lifting platform, and a first piston block adapted to the size of the first piston chamber is fixed to the bottom end of the first column rod, a second piston chamber located below the sealing plate is provided in the main body, a second column rod vertically inserted in the second piston chamber is fixed to the bottom of the sealing plate, and a second piston block adapted to the size of the second piston chamber is fixed to the bottom end of the second column rod, the bottom of the second piston chamber is connected to the bottom of the first piston chamber and is filled with oil.

[0013] Preferably, a rotating shaft is rotatably installed in the disinfection chamber, and the rotating shaft is fixedly connected to the card holder. The card holder is rotatably installed in the disinfection chamber through the rotating shaft. A servo motor for driving the rotating shaft to rotate is fixed in the main body, and a third air suction hole facing the microfluidic chip is opened in the card holder.

[0014] Preferably, a magnetic disk is fixed at the end of the rotating shaft, a Hall sensor is installed in the main body and is sleeved on the outside of the magnetic disk, a worm wheel is fixed on the rotating shaft, and a worm is engaged on the outside of the worm wheel, and the worm is fixedly connected to the drive shaft of the servo motor.

[0015] Preferably, a vacuum generator is fixed in the main body, and the air inlet of the vacuum generator is connected to the air outlet of the air pump, and the air outlet of the vacuum generator is connected to the air supply pipe, and a first socket parallel to the second electric push rod is provided in the main body, a first plug rod slidably inserted in the first socket is fixed on the connecting block, and a channel connected to the first suction hole and the first socket is passed through the first plug rod, a second socket parallel to the second suction hole is provided in the main body, a second plug rod slidably inserted in the second socket is fixed at the bottom of the second suction hole, and a channel connected to the second suction hole and the second socket is passed through the second plug rod, a channel connected to the third suction hole is opened in the rotating shaft, a sleeve movably sleeved on the outside of the rotating shaft is fixed in the disinfection chamber, and a through hole connected between the channel in the rotating shaft and the sleeve is opened on the end wall of the rotating shaft, the negative pressure exhaust port of the vacuum generator is respectively connected to the first socket, the second socket and the sleeve, and are respectively provided with electric control valves.

[0016] Preferably, a connecting rod is inserted into the drain outlet, a float is fixed to the top end of the connecting rod, and a limit block is fixed to the bottom end of the connecting rod.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. In the present invention, a disinfection chamber is provided on one side of the detection chamber, and a through groove is provided to connect the detection chamber and the disinfection chamber. The microfluidic chip can be moved back and forth between the detection chamber and the disinfection chamber by cooperating with the traction mechanism to move the microfluidic chip laterally. This allows the device to clean and disinfect the microfluidic chip in advance before testing infectious pathogen samples, and directly load it into the detection chamber without contact with the outside world. This can avoid the impact of contamination of the microfluidic chip during manual loading on the test results, which is conducive to ensuring the accuracy of the test. Moreover, after the test is completed, the used microfluidic chip can be automatically transferred to the disinfection chamber for disinfection and cleaning, without the need for manual handling by the operator, which can effectively reduce the probability of the operator contacting the used microfluidic chip, and is conducive to ensuring the safety of the test.

[0019] 2. In the present invention, by hiding the second nozzle in the corresponding first nozzle, when the device sprays disinfectant onto the microfluidic chip in the disinfection chamber through the first nozzle to clean it, the air pump can be started to spray air through the second nozzle. The high-speed airflow can be ejected through the second nozzle hidden in the first nozzle to assist in the diffusion of the disinfectant sprayed from the first nozzle, which can effectively improve the effect of the disinfectant on the microfluidic chip, thereby helping to improve the efficiency and comprehensiveness of the device in cleaning and disinfecting the microfluidic chip.

[0020] 3. In the present invention, the card holder is rotatably installed in the disinfection chamber by a rotating shaft, so that when the detection device cleans and disinfects the microfluidic chip, the card holder can drive the microfluidic chip to rotate continuously, constantly changing the position state of the microfluidic chip, which can effectively improve the comprehensiveness of the microfluidic chip when being cleaned and disinfected. At the same time, by opening the third suction hole in the card holder, with the help of negative pressure adsorption, the microfluidic chip can be prevented from detaching from the card holder during the rotation disinfection process, thereby ensuring the stability of the device when cleaning and disinfecting the microfluidic chip to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A perspective view of the present invention;

[0022] Figure 2 A top view of the present invention;

[0023] Figure 3 For the present invention Figure 2 Cross-sectional view at AA in the middle;

[0024] Figure 4 For the present invention Figure 2 Cross-sectional view at the middle BB;

[0025] Figure 5 For the present invention Figure 2 Cross-sectional view at CC;

[0026] Figure 6 It is a front view of the present invention;

[0027] Figure 7 For the present invention Figure 6 Cross-sectional view at DD in the middle;

[0028] Figure 8 For the present invention Figure 6 Cross-sectional view at EE;

[0029] Figure 9 For the present invention Figure 6 Cross-sectional view at FF;

[0030] Figure 10 This is an exploded view of the card holder and microfluidic chip of the present invention;

[0031] Figure 11 A perspective view of the first nozzle and the second nozzle of the present invention;

[0032] Figure 12 A three-dimensional diagram of the sealing plate, the first electric push rod and the rubber bag of the present invention;

[0033] Figure 13 A three-dimensional diagram of the lifting platform and the second air suction hole of the present invention;

[0034] Figure 14 A three-dimensional diagram of the connecting block, the second electric push rod and the first air intake hole of the present invention;

[0035] Figure 15 A perspective view of the air pump and vacuum generator of the present invention;

[0036] Figure 16 It is a three-dimensional diagram of the connecting rod, floating ball and limiting block of the present invention.

[0037] Serial number in the picture:

[0038] 1. Main body; 101. Detection chamber; 102. First cover plate; 103. Loading slot; 104. Connector;

[0039] 2. Disinfection chamber; 201. Second cover plate; 202. Card rack; 203. Liquid supply pipe; 204. First nozzle; 205. Drain port; 206. Air pump; 207. Air supply pipe; 208. Second nozzle; 209. Exhaust pipe;

[0040] 3. Through slot; 301. Closing plate; 302. First electric push rod; 303. Rubber bag;

[0041] 4. Connecting block; 401. Second electric push rod; 402. First air intake hole; 403. First plug hole; 404. First plug rod;

[0042] 5. Lifting platform; 501. Second air intake hole; 502. First piston chamber; 503. First column; 504. First piston block; 505. Second piston chamber; 506. Second column; 507. Second piston block; 508. Second insertion hole; 509. Second insertion rod;

[0043] 6. Rotating shaft; 601. Servo motor; 602. Disk; 603. Hall sensor; 604. Worm gear; 605. Worm; 606. Third air intake port; 607. Sleeve;

[0044] 7. Vacuum generator;

[0045] 8. Connecting rod; 801. Float; 802. Limit block. DETAILED DESCRIPTION

[0046] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0047] Example: This example provides a detection device for detecting infectious pathogens using a microfluidic chip, see Figures 1-16 Specifically, it includes a main body 1, a detection chamber 101 is provided in the main body 1, and a first cover plate 102 is rotatably installed on the detection chamber 101, a loading slot 103 is fixed in the detection chamber 101, and a joint 104 is fixed on the loading slot 103, a disinfection chamber 2 arranged side by side with the detection chamber 101 is provided in the main body 1, and a second cover plate 201 is rotatably installed on the disinfection chamber 2, a card rack 202 arranged corresponding to the loading slot 103 is installed in the disinfection chamber 2, a liquid supply pipe 203 and a plurality of first nozzles 204 connected to the liquid supply pipe 203 are fixed on the second cover plate 201, a drain port 205 is provided at the bottom of the disinfection chamber 2, an air pump 206 is fixed in the main body 1, an air supply pipe 207 and a plurality of second nozzles 208 connected to the air supply pipe 207 are fixed on the second cover plate 201, an electric heating component is provided in the air supply pipe 207, and the disinfection chamber 2 is connected to Exhaust pipe 209, a through slot 3 is provided in the main body 1, which is connected between the detection chamber 101 and the disinfection chamber 2, and the through slot 3 is in the same plane as the card holder 202 and is located above the loading slot 103. A sealing plate 301 is vertically slidably installed in the through slot 3, and a first electric push rod 302 is fixed in the main body 1 for driving the sealing plate 301 to rise and fall. A traction mechanism is provided on the side of the detection chamber 101 away from the disinfection chamber 2, and the traction mechanism includes a connecting block 4 housed in the end wall of the detection chamber 101, and the connecting block 4 is in the same plane as the through slot 3. A second electric push rod 401 is fixed in the main body 1 for driving the connecting block 4 to move horizontally, and a first air suction hole 402 is provided on the side of the connecting block 4 close to the detection chamber 101. A lifting platform 5 arranged below the middle position of the loading slot 103 is installed in the detection chamber 101 for lifting and lowering, and a second air suction hole 501 is provided on the top of the lifting platform 5.

[0048] During the use of the detection device, the operator can use the device to perform detection operations on infectious pathogens. Before the detection, the second cover 201 is flipped upward to open the disinfection chamber 2, and the microfluidic chip is inserted into the card holder 202. Then, the second cover 201 is flipped downward to close the disinfection chamber 2. The liquid supply pipe 203 is connected to the disinfectant supply mechanism. The disinfectant is continuously supplied to the liquid supply pipe 203 through the disinfectant supply mechanism. Then, the special disinfectant for cleaning and disinfecting the microfluidic chip is diverted through the liquid supply pipe 203 to the plurality of first nozzles 204 and sprayed, acting on the card holder 2. The cleaning and disinfection operation of the microfluidic chip is realized on the microfluidic chip plugged in 02, and the sprayed disinfectant will be discharged through the drain port 205 opened at the bottom of the disinfection chamber 2. After the disinfection of the microfluidic chip is completed, the air pump 206 is powered on and started, and through the connection of the air supply pipe 207, the high-speed air flow is sprayed out through the second nozzle 208 to act on the microfluidic chip to perform a blow-drying operation on the microfluidic chip. During the drying process, the electric heating component connected to the air supply pipe 207 can be powered on and started to increase the temperature of the air flow, thereby effectively improving the drying efficiency of the microfluidic chip.

[0049] After the microfluidic chip is dried, the first electric push rod 302 is powered on and started, driving the sealing plate 301 to descend downward in the through groove 3, so that the through groove 3 is in an unobstructed state, and then the second electric push rod 401 is powered on and started, driving the connecting block 4 to move to the left. After the connecting block 4 passes through the through groove 3, it enters the disinfection chamber 2 and abuts against the right end of the microfluidic chip inserted in the card holder 202. Then the air suction in the first suction hole 402 is started, and the microfluidic chip is connected to the connecting block 4 with the help of the negative pressure adsorption force formed by the air suction. Then the second electric push rod 401 is started in the reverse direction to pull the microfluidic chip from the disinfection chamber 2 into the detection chamber 101.

[0050] During the transverse movement of the microfluidic chip, the lifting platform 5 is controlled to rise upward, and the lifting platform 5 and the bottom of the microfluidic chip are in the same plane. By lifting the lifting platform 5, the microfluidic chip can be stably moved transversely to the detection chamber 101. When the microfluidic chip is above the loading slot 103, the first suction hole 402 releases the negative pressure adsorption connection to the microfluidic chip, and the connecting block 4 moves to reset and is stored in the end wall of the connecting block 4, while the second suction hole 501 on the lifting platform 5 performs airflow suction operation, with the help of the negative pressure adsorption formed by airflow suction. The force can firmly connect the bottom of the microfluidic chip with the top of the lifting platform 5, and the lifting platform 5 is controlled to drop downward, so that the microfluidic chip is accurately embedded in the loading slot 103, and the upright interface on the microfluidic chip is plugged into the connector 104. During the installation of the microfluidic chip, the first electric push rod 302 is started in reverse, and the control sealing plate 301 is raised in the through groove 3 to block the through groove 3. After that, the operator can flip the first cover plate 102 upward to open the detection chamber 101, add samples to the microfluidic chip, and perform the detection operation of infectious pathogens.

[0051] When the detection operation is completed, the sealing plate 301 is controlled to drop downward again, so that the through groove 3 is opened. Synchronously, the lifting platform 5 rises upward, lifting the used microfluidic chip upward and placing it in a state flush with the through groove 3. After being lifted by the lifting platform 5, the interface on the microfluidic chip is separated from the connector 104, and then the traction mechanism is started to drive the connecting block 4 to move horizontally toward the disinfection chamber 2, pushing the used microfluidic chip into the card rack 202 through the through groove 3, and then the sealing plate 301, the connecting block 4 and the lifting platform 5 are reset, and the through groove 3 is re-closed. In this state, the device can spray disinfectant through the first nozzle 204 to achieve disinfection and cleaning of the used microfluidic chip, and after disinfection, the second nozzle 208 sprays air flow for blow-drying. Then the operator can flip the second cover 201 upward to open the disinfection chamber 2 and take out the cleaned and disinfected microfluidic chip for storage.

[0052] During use of the device, a disinfection chamber 2 is provided on one side of the detection chamber 101, and a through groove 3 is provided to connect the detection chamber 101 and the disinfection chamber 2. The traction mechanism is used to move the microfluidic chip laterally, so that the microfluidic chip can move back and forth between the detection chamber 101 and the disinfection chamber 2. This allows the device to clean and disinfect the microfluidic chip in advance before testing infectious pathogen samples, and load it into the detection chamber 101 in a manner that does not contact the outside world. This can effectively prevent the microfluidic chip from being contaminated during manual loading and affecting the test results, which is beneficial to ensuring the accuracy of the test. Moreover, after the test is completed, the used microfluidic chip can be automatically transferred to the disinfection chamber 2 for disinfection and cleaning, without the need for manual handling by the operator. The operation is convenient, safe and effective.

[0053] In the specific implementation process, Figure 1 、 Figure 3 and Figure 11 As shown, a second nozzle 208 is inserted into a first nozzle 204, and the second nozzle 208 is coaxially arranged with the corresponding first nozzle 204. The second nozzle 208 is hidden inside the corresponding first nozzle 204. During the use of the detection device, by hiding the second nozzle 208 in the corresponding first nozzle 204, the device can spray disinfectant to the microfluidic chip in the disinfection chamber 2 through the first nozzle 204 for cleaning. At the same time, the air pump 206 can also be started to spray air through the second nozzle 208. The high-speed airflow is ejected through the second nozzle 208 hidden in the first nozzle 204, which can assist the diffusion of the disinfectant sprayed from the first nozzle 204, and can effectively improve the effect of the disinfectant on the microfluidic chip, thereby helping to improve the efficiency and comprehensiveness of the device in cleaning and disinfecting the microfluidic chip.

[0054] In the specific implementation process, Figure 5、 Figure 9 and Figure 12 As shown, a rubber bag 303 is fixed on the side of the sealing plate 301 close to the disinfection chamber 2, and the rubber bag 303 is filled with argon. During the use of the detection device, when the sealing plate 301 is sealed in the through groove 3, the rubber bag 303 is tightly filled in the through groove 3 on the side close to the disinfection chamber 2, so that the through groove 3 is completely sealed and blocked, which is conducive to improving the sealing performance of the through groove 3 when it is closed. When the microfluidic chip is cleaned and disinfected in the disinfection chamber 2, in order to improve the cleaning and disinfection effect, the electric heating component can be started to clean the disinfection chamber 2. During the heating operation, the argon gas filled in the rubber bag 303 can block the heat and prevent the heat from being transferred to the detection cavity 101. At the same time, the argon gas filled in the rubber bag 303 will expand when heated, driving the rubber bag 303 to further expand outward, so that the rubber bag 303 can be more tightly filled in the through groove 3, ensuring the sealing when the through groove 3 is closed. The sealing plate 301 is made of heat-insulating material. The sealing plate 301 cooperates with the argon gas in the rubber bag 303 to further enhance the heat insulation effect of the through groove 3.

[0055] In the specific implementation process, Figure 4 and Figure 7 As shown, a first piston chamber 502 located below the lifting platform 5 is provided in the main body 1, a first column rod 503 vertically inserted in the first piston chamber 502 is fixed to the bottom of the lifting platform 5, and a first piston block 504 adapted to the size of the first piston chamber 502 is fixed at the bottom end of the first column rod 503, a second piston chamber 505 located below the sealing plate 301 is provided in the main body 1, a second column rod 506 vertically inserted in the second piston chamber 505 is fixed to the bottom of the sealing plate 301, and a second piston block 507 adapted to the size of the second piston chamber 505 is fixed at the bottom end of the second column rod 506, the bottom of the second piston chamber 505 is connected to the bottom of the first piston chamber 502, and is filled with oil.

[0056] When the detection device is used, in the initial state, the sealing plate 301 is sealed inside the through groove 3. At this time, the second piston block 507 is in the upper position in the second piston chamber 505, and the first piston block 504 is in the lower position in the first piston chamber 502. The oil is below the second piston block 507 in the second piston chamber 505, below the first piston block 504 in the first piston chamber 502, and in the connecting pipeline. When the first electric push rod 302 drives the sealing plate 301 to descend and open in the through groove 3, the second column rod 506 will drive the second piston block 507 to move downward. The plug 507 moves downward in the second piston chamber 505, pushing the oil in the second piston chamber 505 into the first piston chamber 502, supporting the first piston block 504 upward, and driving the lifting platform 5 to rise upward through the connection of the first column rod 503 to lift the microfluidic chip. When the sealing plate 301 is controlled to rise upward, the lifting platform 5 is controlled to fall downward in linkage. Through the flow of oil, the lifting linkage control of the sealing plate 301 and the lifting platform 5 is realized, which can effectively improve the convenience and flexibility of the device in actual use.

[0057] In the specific implementation process, Figure 1 、 Figure 3 、 Figure 9 and Figure 10 As shown, a rotating shaft 6 is rotatably installed in the disinfection chamber 2, and the rotating shaft 6 is fixedly connected to the card holder 202. The card holder 202 is rotatably installed in the disinfection chamber 2 through the rotating shaft 6. A servo motor 601 for driving the rotating shaft 6 to rotate is fixed in the main body 1. A third air suction hole 606 facing the microfluidic chip is provided in the card holder 202. During the use of the detection device, when the microfluidic chip is cleaned and disinfected, the servo motor 601 is powered on and started, which will drive the rotating shaft 6 to drive the card holder 202 to rotate, and then the card holder 202 drives the microfluidic chip to rotate in the disinfection chamber 2. By continuously rotating and constantly changing the position state of the microfluidic chip, the comprehensiveness of the microfluidic chip when being cleaned and disinfected can be effectively improved. During the rotation disinfection process, in order to prevent the microfluidic chip from detaching from the card holder 202, the third air suction hole 606 provided in the card holder 202 will be started to perform airflow suction operation. The negative pressure adsorption force formed by the airflow suction allows the microfluidic chip to be stably stored in the card holder 202, which to a certain extent ensures the stability of the device when cleaning and disinfecting the microfluidic chip.

[0058] In the specific implementation process, Figure 3 、 Figure 9 and Figure 10As shown, a magnetic disk 602 is fixed at the end of the rotating shaft 6, a Hall sensor 603 is installed in the main body 1 and is sleeved on the outside of the magnetic disk 602, a worm gear 604 is fixed on the rotating shaft 6, and a worm 605 is meshed on the outside of the worm gear 604, and the worm 605 is fixedly connected to the drive shaft of the servo motor 601. During the use of the detection device, the rotation posture of the rotating shaft 6 can be determined by the cooperation of the magnetic disk 602 and the Hall sensor 603, thereby realizing the positioning of the posture of the card holder 202. When the microfluidic chip is moved laterally, the card holder 202 is controlled to be in a horizontal state. In the upright state, the opening on the right side of the card holder 202 is aligned with the through slot 3, which can ensure the smooth and stable transverse movement of the microfluidic chip between the detection chamber 101 and the disinfection chamber 2. When the microfluidic chip needs to be inserted into the card holder 202 or removed from the card holder 202, the card holder 202 can be kept in an upright state through the rotation of the rotating shaft 6 and the coordinated positioning of the magnetic disk 602 and the Hall sensor 603. In this state, the opening of the card holder 202 is adjusted to the upper position, and the operator can take and place the microfluidic chip vertically, making the taking and placing of the microfluidic chip convenient.

[0059] When the servo motor 601 drives the rotating shaft 6 to rotate the card holder 202, the drive shaft of the servo motor 601 rotates, driving the worm 605 to rotate. Then, with the help of the engagement of the worm 605 and the worm wheel 604, the rotating shaft 6 is driven to rotate, providing power for the rotation of the card holder 202. In this process, with the help of the self-locking property of the meshing transmission of the worm wheel 604 and the worm 605, the card holder 202 is automatically locked after the rotation adjustment, which can effectively ensure the structural stability of the card holder 202 when the horizontal and vertical postures are adjusted.

[0060] In the specific implementation process, Figure 4 、 Figure 9 and Figure 15As shown, a vacuum generator 7 is fixed in the main body 1, and the air inlet of the vacuum generator 7 is communicated with the air outlet of the air pump 206, and the air outlet of the vacuum generator 7 is communicated with the air supply pipe 207. A first socket 403 parallel to the second electric push rod 401 is provided in the main body 1, and a first plug rod 404 slidably inserted in the first socket 403 is fixed on the connecting block 4, and a channel connected between the first suction hole 402 and the first socket 403 is passed through the first plug rod 404, and a second socket 508 parallel to the second suction hole 501 is provided in the main body 1. A second plug rod 509 is fixed to the bottom of the body 502, which is slidably inserted into the second socket 508. A channel connecting the second air suction hole 501 and the second socket 508 is passed through the second plug rod 509. A channel connected to the third air suction hole 606 is provided in the rotating shaft 6. A sleeve 607 movably sleeved on the outside of the rotating shaft 6 is fixed in the disinfection chamber 2. A through hole connecting the channel in the rotating shaft 6 and the sleeve 607 is provided on the end wall of the rotating shaft 6. The negative pressure exhaust port of the vacuum generator 7 is respectively connected to the first socket 403, the second socket 508 and the sleeve 607, and is respectively provided with an electrically controlled valve.

[0061] During the use of the detection device, the first plug rod 404 is slidably inserted into the first socket 403 to ensure the stability of the lateral movement of the connecting block 4, and the second plug rod 509 is slidably inserted into the second socket 508 to ensure the stability of the lifting platform 5 in the up and down movement. The device connects the vacuum generator 7 to the air outlet of the air pump 206. After the high-speed airflow ejected from the air outlet of the air pump 206 passes through the vacuum generator 7, an airflow suction effect is formed at the negative pressure exhaust port of the vacuum generator 7 under the Bernoulli effect. The mixed airflow is ejected through the air outlet of the vacuum generator 7. This airflow can be connected to the second nozzle 208 through the air supply pipe 207. The air flow is ejected and acts in the disinfection chamber 2, while the air flow suction at the negative pressure suction port on the vacuum generator 7 acts in the first jack 403, the second jack 508 and the sleeve 607 in sequence through the connection of the pipeline, and then is implemented at the first suction hole 402, the second suction hole 501 and the third suction hole 606 through the connection of the first plug rod 404, the second plug rod 509 and the channel in the rotating shaft 6, and is independently controlled by the corresponding electric control valves. By providing the vacuum generator 7, the supply of air flow and the suction of air flow at many positions can come from the air pump 206, which can effectively improve the stability and energy saving of the device during operation, and the operation is flexible and convenient.

[0062] In the specific implementation process, Figure 3 、 Figure 5 and Figure 16As shown, a connecting rod 8 is inserted into the drain outlet 205, and a float 801 is fixed to the top of the connecting rod 8, and a limit block 802 is fixed to the bottom of the connecting rod 8. During the use of the detection device, when there is no liquid in the disinfection chamber 2, the float 801 will be blocked at the top of the drain outlet 205 under the action of gravity, closing the drain outlet 205. In this state, the gas sprayed from the second nozzle 208 will not leak outward through the drain outlet 205, but can only be discharged outward through the exhaust pipe 209. When there is liquid in the disinfection chamber 2, the connecting rod 8 will float in the liquid, causing the drain outlet 205 to open, and then the liquid is discharged outward through the drain outlet 205. In this state, the liquid gathers at the bottom of the disinfection chamber 2, and the gas sprayed from the second nozzle 208 will not leak outward through the drain outlet 205, thereby avoiding the abnormal discharge of waste gas through the drain outlet 205, which is conducive to ensuring the safety and stability of the device during use.

[0063] Specifically, the working principle and operation method of the present invention are as follows:

[0064] The device drives the rotating shaft 6 to rotate the card rack 202 through the servo motor 601, so that the card rack 202 stands upright with the opening facing upward. The operator flips up the second cover 201 to open the disinfection chamber 2, inserts the microfluidic chip to be used into the card rack 202, and the air pump 206 starts to suck the air into the third suction hole 606 through the conversion of the vacuum generator 7 to ensure that the temperature of the microfluidic chip is kept within the card rack 202. Then, the second cover 201 is flipped down to close the disinfection chamber 2, and the disinfectant supply mechanism continuously supplies the disinfectant to the plurality of first nozzles 204 through the liquid supply pipe 203. The disinfectant is continuously supplied to disinfect and clean the microfluidic chip in the card holder 202 by spraying the disinfectant. Synchronously, the servo motor 601 drives the shaft 6 to drive the card holder 202 to rotate, driving the microfluidic chip to rotate, and the high-speed airflow provided by the air pump 206 is sprayed from the second nozzle 208 through the connection between the vacuum generator 7 and the air supply pipe 207, thereby enhancing the range and impact of the disinfectant spraying. With the cooperation of each other, the comprehensiveness and efficiency of the disinfection and cleaning of the microfluidic chip are improved. After the disinfection is completed, the first nozzle 204 stops spraying the disinfectant, and the second nozzle 204 stops spraying the disinfectant. 08 continues to start, and the air flow is heated by the electric heating component to achieve efficient drying operation of the microfluidic chip, and then the card holder 202 drives the microfluidic chip to maintain a horizontal state, the first electric push rod 302 controls the sealing plate 301 to fall in the through groove 3, and the second electric push rod 401 controls the connection block 4 to move left and right, cooperates with the negative pressure adsorption connection at the first suction hole 402, and the lifting of the lifting platform 5 to lift the microfluidic chip in the card holder 202 horizontally to the top of the loading slot 103, and then the air flow suction at the second suction hole 501 lifts the lifting platform 5 and the microfluidic chip. The microfluidic chip is connected to the body chip, and the microfluidic chip is accurately loaded into the loading slot 103 by the drop of the lifting platform 5. The operator flips up the first cover 102 to open the detection chamber 101, and the infectious pathogen sample can be added to the microfluidic chip. The operator flips down the first cover 102 to close the detection chamber 101 and uses the device to perform detection operations on the sample. After the detection is completed, the above operations are reversed and the used microfluidic chip is sent to the card rack 202 in the disinfection chamber 2 for disinfection. After the disinfection is completed, the operator takes out the microfluidic chip for storage.

[0065] The above are only preferred specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with this technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A detection device for detecting infectious pathogens using a microfluidic chip, comprising a main body (1), characterized in that: The main body (1) is provided with a detection chamber (101), and a first cover plate (102) is rotatably mounted on the detection chamber (101), a loading slot (103) is fixed in the detection chamber (101), and a joint (104) is fixed on the loading slot (103), a disinfection chamber (2) is provided in the main body (1), and a second cover plate (201) is rotatably mounted on the disinfection chamber (2), a card rack (202) is installed in the disinfection chamber (2), a liquid supply pipe (203) and a plurality of first nozzles (204) are fixed on the second cover plate (201), a drain port (205) is provided at the bottom of the disinfection chamber (2), and an air pump ( 206), an air supply pipe (207) and a plurality of second nozzles (208) are fixed on the second cover plate (201), an exhaust pipe (209) is connected to the disinfection chamber (2), a through slot (3) is provided in the main body (1) and is connected between the detection chamber (101) and the disinfection chamber (2), and the through slot (3) and the card holder (202) are in the same plane and are located above the loading slot (103), a sealing plate (301) is vertically slidable in the through slot (3), a first electric push rod (302) for driving the sealing plate (301) to rise and fall is fixed in the main body (1), and a traction mechanism is provided on the side of the detection chamber (101) away from the disinfection chamber (2); The traction mechanism comprises a connecting block (4) housed in an end wall of the detection cavity (101), and the connecting block (4) and the through slot (3) are located in the same plane. A second electric push rod (401) for driving the connecting block (4) to move laterally is fixed in the main body (1), and a first air suction hole (402) is provided on a side of the connecting block (4) close to the detection cavity (101); A lifting platform (5) is installed in the detection chamber (101) and is arranged below the middle position of the loading slot (103). A second air suction hole (501) is provided on the top of the lifting platform (5); The main body (1) is provided with a first piston chamber (502) located below the lifting platform (5), the bottom of the lifting platform (5) is fixed with a first column (503) vertically inserted in the first piston chamber (502), and the bottom end of the first column (503) is fixed with a first piston block (504) adapted to the size of the first piston chamber (502), the main body (1) is provided with a second piston chamber (505) located below the sealing plate (301), the bottom of the sealing plate (301) is fixed with a second column (506) vertically inserted in the second piston chamber (505), and the bottom end of the second column (506) is fixed with a second piston block (507) adapted to the size of the second piston chamber (505), the bottom of the second piston chamber (505) is connected to the bottom of the first piston chamber (502), and is filled with oil; A rotating shaft (6) is rotatably mounted in the disinfection chamber (2), and the rotating shaft (6) is fixedly connected to the card frame (202). The card frame (202) is rotatably mounted in the disinfection chamber (2) via the rotating shaft (6). A servo motor (601) for driving the rotating shaft (6) to rotate is fixed in the main body (1). A third air suction hole (606) facing the microfluidic chip is provided in the card frame (202).

2. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A second nozzle (208) is inserted into a first nozzle (204), the second nozzle (208) is coaxially arranged with the corresponding first nozzle (204), and the second nozzle (208) is hidden inside the corresponding first nozzle (204).

3. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A rubber bag (303) is fixed on one side of the sealing plate (301) close to the disinfection chamber (2), and the rubber bag (303) is filled with argon gas.

4. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A magnetic disk (602) is fixed at the end of the rotating shaft (6), a Hall sensor (603) is installed in the main body (1) and is sleeved on the outside of the magnetic disk (602), a worm wheel (604) is fixed on the rotating shaft (6), and a worm (605) is meshed on the outside of the worm wheel (604), and the worm (605) is fixedly connected to the drive shaft of the servo motor (601).

5. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A vacuum generator (7) is fixed in the main body (1), and the air inlet of the vacuum generator (7) is communicated with the air outlet of the air pump (206), and the air outlet of the vacuum generator (7) is communicated with the air supply pipe (207). A first jack (403) parallel to the second electric push rod (401) is provided in the main body (1), a first plug rod (404) slidably inserted in the first jack (403) is fixed on the connecting block (4), and a passage connecting the first air intake hole (402) and the first jack (403) is passed through the first plug rod (404), a second jack (508) parallel to the second air intake hole (501) is provided in the main body (1), and the second air intake hole (501) is connected to the first air intake hole (501). 01) is fixed with a second plug rod (509) which is slidably inserted into the second plug hole (508), and a channel connecting the second air suction hole (501) and the second plug hole (508) is passed through the second plug rod (509), a channel connected to the third air suction hole (606) is provided in the rotating shaft (6), a sleeve (607) which is movably sleeved on the outside of the rotating shaft (6) is fixed in the disinfection chamber (2), and a through hole connecting the channel in the rotating shaft (6) and the sleeve (607) is provided on the end wall of the rotating shaft (6), and the negative pressure suction port of the vacuum generator (7) is respectively connected to the first plug hole (403), the second plug hole (508) and the sleeve (607), and is respectively provided with an electric control valve.

6. The detection device for detecting infectious pathogens using a microfluidic chip according to claim 1, characterized in that: A connecting rod (8) is inserted into the drain outlet (205), a floating ball (801) is fixed to the top end of the connecting rod (8), and a limiting block (802) is fixed to the bottom end of the connecting rod (8).

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