Micromanipulation device

Abstract

A micromanipulation device comprises a microscopic image capturing mechanism and an operating mechanism which are arranged on a connecting seat mechanism. The microscopic image capturing mechanism comprises a microscopic image capturing unit which can be shifted up and down and is provided with two spaced microscopic lenses. The operating mechanism comprises an operating unit which can be moved up and down, and the operating unit can be driven to move down into the view field of the microscope lens and perform predetermined treatment on the biological sample in the sample container below. Through the structural design that the microscopic image taking mechanism is provided with two microscope lenses and the operating tool of the operating mechanism is located in the visual field of the microscope lenses, the relative position relation between the operating unit and the biological sample can be observed from two different visual field angles; and the biological sample can be processed more accurately.

Description

Technical Field

[0001] The present invention relates to an operating device, and particularly to a micromanipulation device for performing a predetermined processing operation on a biological sample through a microscopic image. Background Art

[0002] In the field of artificial reproduction, after an egg is fertilized in vitro, the fertilized egg is implanted into the uterine body for development. When performing artificial fertilization, medical staff need to screen viable sperm or eggs from a culture container, or take out the fertilized eggs from the culture container. Since sperm, eggs, and fertilized eggs are all very tiny, it is necessary to rely on microscopic imaging technology to find the required sperm, eggs, or fertilized eggs in the culture container. Then, with the assistance of the microscopic image, the tip of a micropipette is manually moved into the corresponding position in the culture container, and the required sperm, eggs, or fertilized eggs are aspirated. However, this manual sampling method is prone to inaccurate sampling and other processing operations due to hand shaking. Summary of the Invention

[0003] An object of the present invention is to provide a micromanipulation device that can improve at least one drawback of the prior art.

[0004] The micromanipulation device of the present invention is applicable to performing microscopic imaging and a predetermined processing operation on a biological sample in a sample container. The micromanipulation device includes a connection base mechanism, and a microscopic imaging mechanism and an operating mechanism mounted on the connection base mechanism.

[0005] The microscopic imaging mechanism includes a first lifting and shifting unit mounted on the connection base mechanism, and a microscopic imaging unit mounted on the first lifting and shifting unit. The first lifting and shifting unit can be controlled to start and vertically shift the microscopic imaging unit relative to the sample container. The microscopic imaging unit includes two spaced microscopic lenses, and the viewing directions of the microscopic lenses intersect. Each microscopic lens can be used to perform microscopic imaging on the biological sample in the sample container.

[0006] The operating mechanism includes a second lifting and shifting unit mounted on the connection base mechanism, and an operating unit that can be vertically shifted by the second lifting and shifting unit and can be used to perform the predetermined processing operation on the biological sample in the sample container. The second lifting and shifting unit is controlled to start and drive the operating unit to move down into the field of view of the microscopic lens and insert downward into the sample container.

[0007] For the micromanipulation device of the present invention, the microscopic imaging unit further includes a telescopic base mounted on the first lifting and shifting unit, two telescopic rods mounted on the telescopic base and protruding outside the telescopic base, and a telescopic drive module mounted on the telescopic base and connected to the telescopic rods. The telescopic drive module can be controlled to start, and drive each telescopic rod to perform telescopic displacement relative to the telescopic base. The microscopic lenses are respectively mounted on the telescopic rods and can be driven to displace individually by the telescopic displacement of the telescopic rods.

[0008] For the micromanipulation device of the present invention, the connecting seat mechanism includes a connecting seat, a rotating seat rotatably mounted on the connecting seat along a horizontal axis, and a rotation driver mounted on the connecting seat and connected to the rotating seat. The first lifting and shifting unit and the second lifting and shifting unit are mounted on the rotating seat. The rotation driver can be controlled to start, and drive the rotating seat to drive the first lifting and shifting unit and the second lifting and shifting unit to rotate relative to the connecting seat.

[0009] The micromanipulation device of the present invention further includes a shifting mechanism connected to the connecting seat. The shifting mechanism includes two first shifting modules spaced parallel in a horizontal first direction, and a second shifting module horizontally extending across the first shifting modules in the first direction. Each first shifting module includes a first transmission screw and a first slide rail spaced parallel and extending in a second direction horizontally orthogonal to the first direction, a first moving seat displaceably sleeved on the first slide rail and screwed to the first transmission screw, and a first rotator connected to the first transmission screw. The first transmission screw can be rotated by the first rotator, and drive the first moving seat to displace along the first slide rail. The second shifting module straddles the first moving seats and is connected to the connecting seat, and can be driven by the first moving seats to shift the connecting seat mechanism relative to the sample container.

[0010] For the micromanipulation device of the present invention, the second shifting module includes a second transmission screw and a second slide rail spaced parallel and extending across the first moving seats in the first direction, a second moving seat displaceably sleeved on the second slide rail and screwed to the second transmission screw, and a second rotator mounted on one of the first moving seats and connected to the second transmission screw. The second moving seat is connected to the connecting seat. The second transmission screw can be rotated by the second rotator, and drive the second moving seat to displace along the second slide rail.

[0011] For the micromanipulation device of the present invention, the first lifting and shifting unit includes a first base installed and fixed on the connecting seat mechanism, a first lifting seat displaceable vertically on the first base, and a first lifting driver installed on the first base and connected to the first lifting seat. The microscopic imaging unit is installed on the first lifting seat, and the first lifting seat can be driven by the first lifting driver to vertically shift the microscopic imaging unit relative to the first base.

[0012] For the micromanipulation device of the present invention, the operating mechanism further includes a third lifting and shifting unit installed between the second lifting and shifting unit and the operating unit. The second lifting and shifting unit can be driven to vertically shift the operating unit via the third lifting and shifting unit. The third lifting and shifting unit can be driven to vertically shift the operating unit relative to the second lifting and shifting unit. The second lifting and shifting unit and the third lifting and shifting unit are driven to have different leads for vertically shifting the operating unit.

[0013] For the micromanipulation device of the present invention, the second lifting and shifting unit includes a second base installed on the connecting seat mechanism, a second lifting seat displaceable vertically on the second base, and a second lifting driver installed on one of the second base and the second lifting seat and connected to the other. The third lifting and shifting unit is installed on the second lifting seat. The second lifting driver can be controlled to start and drive the second lifting seat to vertically displace relative to the second base, thereby vertically shifting the third lifting and shifting unit and the operating unit.

[0014] For the micromanipulation device of the present invention, the second lifting driver includes a second transmission rack extending vertically and fixed on the second lifting seat, and a second lifting drive motor installed and fixed on the second base and meshing with the second transmission rack. The second lifting drive motor can be controlled to start and drive the second transmission rack to drive the second lifting seat to vertically displace relative to the second base.

[0015] For the micromanipulation device of the present invention, the third lifting and shifting unit includes a third base installed on the second lifting seat, a third lifting seat displaceable vertically on the third base, and a third lifting driver installed on one of the third lifting seat and the third base and connected to the other. The third lifting driver can be controlled to start and drive the third lifting seat to vertically displace relative to the third base. The operating unit is installed on the third lifting seat.

[0016] For the micromanipulation device of the present invention, the third lifting driver includes a third transmission rack fixedly extending up and down on the third base, a third lifting drive motor installed on the third lifting seat, and a reduction gear set installed on the third lifting seat and connected between the third lifting drive motor and the third transmission rack.

[0017] The micromanipulation device of the present invention further includes an imaging lens installed on the bottom end surface of the third lifting seat and capable of capturing an image downward toward the bottom end of the operation unit.

[0018] The beneficial effects of the present invention are as follows: Through the structural design of the microscope lens of the microscopic imaging mechanism and the structural design that the operation unit can be shifted into the field of view of the microscope lens, the relative positional relationship between the operation unit and the biological sample can be observed using microscope lenses with different field angles, and thus the biological sample can be more precisely subjected to predetermined processing. Description of the Drawings

[0019] Other features and effects of the present invention will be clearly presented in the embodiments with reference to the drawings, where:

[0020] Figure 1 is an incomplete perspective view showing the structure of an embodiment of the micromanipulation device of the present invention;

[0021] Figure 2 is an incomplete side sectional view showing the situation when a microscopic imaging mechanism and an operating mechanism of this embodiment are used with a sample container containing a biological sample;

[0022] Figure 3 is a perspective view showing the structures of the microscopic imaging mechanism and the operating mechanism of this embodiment;

[0023] Figure 4 is a perspective view showing the structures of the microscopic imaging mechanism and the operating mechanism of this embodiment;

[0024] Figure 5 is a front view showing the structure of this embodiment;

[0025] Figure 6 is an exploded perspective view showing the structure of the microscopic imaging mechanism of this embodiment;

[0026] Figure 7 is an exploded perspective view showing the structure of a second lifting and shifting unit of the operation unit of this embodiment;

[0027] Figure 8 is an exploded perspective view showing the structure of a third lifting and shifting unit of the operation unit of this embodiment; and

[0028] Figure 9 is an incomplete side sectional view showing the situation when the microscopic imaging mechanism of this embodiment samples the sample container. Detailed implementation manners

[0029] Refer to Figure 1 、 2 and, an embodiment of the microscopic operation device 200 of the present invention is applicable to perform operation processes such as but not limited to sampling, injection, etching, and laser drilling on a biological sample 901 contained in a sample container 900. The biological sample 901 is, for example but not limited to, sperm, egg, or fertilized egg. The microscopic operation device 200 includes a transfer mechanism 3, a connection seat mechanism 4 mounted on the transfer mechanism 3, a microscopic imaging mechanism 5 and an operation mechanism 6 mounted on the connection seat mechanism 4, and an imaging lens 7 mounted on the operation mechanism 6.

[0030] The transfer mechanism 3 includes two first transfer modules 31 that are spaced parallel in a first direction 801 and extend along a second direction 802 that is horizontally orthogonal to the first direction 801, and a second transfer module 32 that extends along the first direction 801 and straddles the first transfer modules 31.

[0031] Each first transfer module 31 includes two first fixed seats 311 that are spaced left and right in the second direction 802, two first slide rails 312 that extend along the second direction 802 and straddle between the first fixed seats 311 and are spaced parallel front and back in the first direction 801, a first transmission screw 313 that extends along the second direction 802 and rotatably straddles between the first fixed seats 311, a first moving seat 314 that is displaceably mounted on the first slide rails 312 and the first transmission screw 313 along the second direction 802, and a first driver 315 that is mounted on one of the first fixed seats 311 and is connected to the first transmission screw 313. The first moving seat 314 is screwed to the first transmission screw 313. The first driver 315 can be controlled to start and drive the first transmission screw 313 to rotate around its own axis, and the driven first transmission screw 313 will drive the first moving seat 314 to displace along the longitudinal direction of the first slide rails 312.

[0032] The second displacement adjustment module 32 is spanned between the first moving seats 314 and can be driven by the first moving seats 314 to displace along the second direction 802. The second displacement adjustment module 32 includes two second slide rails 321 that are spaced parallel in the second direction 802 and extend along the first direction 801 and span across the first moving seats 314, a second driving screw 322 that extends along the first direction 801 and is rotatably spanned across the first moving seats 314, a second moving seat 323 that is displaceable along the first direction 801 and is mounted on the second slide rails 321 and the second driving screw 322, and a second rotator 324 that is mounted on one of the second moving seats 323 and is connected to the second driving screw 322. The second moving seat 323 is screwed to the second driving screw 322. The second rotator 324 can be controlled to start and drive the second driving screw 322 to rotate around its own axis. The driven second driving screw 322 will drive the second moving seat 323 to displace along the second slide rails 321.

[0033] In this embodiment, both the first rotator 315 and the second rotator 324 are transmission members formed by connecting a motor and a reduction gear set. The motor is, for example but not limited to, a stepper motor. Since both the first rotator 315 and the second rotator 324 used to respectively drive the first driving screw 313 and the second driving screw 322 are prior arts and there are many types, they will not be described in detail herein, and are not limited to the icon styles.

[0034] Refer to Figure 1 、 3 Figures 4 and 5, the connecting seat mechanism 4 is mounted on the second moving seat 323 and can be driven by the second moving seat 323 to perform horizontal displacement. The connecting seat mechanism 4 includes a connecting seat 41 mounted below the second moving seat 323, a rotating seat 42 rotatably mounted on the connecting seat 41 along a horizontal axis, and a rotation driver 43 mounted on the connecting seat 41 and connected to the rotating seat 42. The rotation driver 43 can be controlled to start and drive the rotating seat 42 to rotate relative to the connecting seat 41. In this embodiment, the horizontal axis is coaxial with the first direction 801.

[0035] In this embodiment, the rotation driver 43 includes a rotation driving motor 431 fixedly mounted on the connecting seat 41 and a reduction gear structure 432 disposed between the rotation driving motor 431 and the rotating seat 42. The rotation driving motor 431 is, for example but not limited to, a stepper motor and can drive the rotating seat 42 to rotate relative to the connecting seat 41 via the reduction gear structure 432. Since the rotation driver 43 that can be used to drive the rotating seat 42 to rotate relative to the connecting seat 41 is a prior art and there are many types, it will not be described in detail herein, and is not limited to the icon styles.

[0036] Refer to Figure 2 、 3 、6, the microscopic imaging mechanism 5 is installed on the rotating base 42 and can be driven by the rotating base 42 to rotate. The microscopic imaging mechanism 5 includes a first lifting and shifting unit 51 installed on the rotating base 42, and a microscopic imaging unit 52 installed on the first lifting and shifting unit 51.

[0037] The first lifting and shifting unit 51 includes a first base 511, a first lifting seat 512 extending vertically and displaceable vertically on the first base 511, and a first lifting driver 513 installed on the first base 511 and connected to the first lifting seat 512. The first lifting driver 513 includes a first transmission rack 514 installed on the first lifting seat 512, a first lifting drive motor 515 installed on the first base 511, and a reduction gear set 516 meshed and connected between the first lifting drive motor 515 and the first transmission rack 514. The first lifting drive motor 515 can be controlled to start and drive the reduction gear set 516, thereby driving the first transmission rack 514 to drive the first lifting seat 512 to displace vertically relative to the first base 511, and adjusting the length of the first lifting seat 512 protruding downward relative to the first base 511. In this embodiment, the first lifting drive motor 515 is a stepping motor, which can cooperate with the design of the reduction gear set 516 to finely adjust the lifting distance of the first lifting seat 512. Since there are many types of the first lifting driver 513, it will not be described in detail, and the implementation is not limited to the illustrated form.

[0038] The microscopic imaging unit 52 includes a telescopic base 521 installed at the bottom end of the first lifting seat 512 and extending along the first direction 801, two telescopic rods 522 inserted into the telescopic base 521 and extending outwards from both ends of the telescopic base 521 in opposite directions along the first direction 801, a telescopic drive module 523 installed on the telescopic base 521 and connected to the telescopic rods 522, and two microscopic lenses 524 respectively installed on the telescopic rods 522.

[0039] The telescopic drive module 523 can be controlled to start and drive each telescopic rod 522 to displace telescopically relative to the telescopic base 521, and adjust the length of the telescopic rod 522 protruding out of the telescopic base 521, thereby shifting the corresponding microscopic lens 524 along the first direction 801. The telescopic drive module 523 is also a transmission member composed of a motor and a reduction gear set connected between the motor and the telescopic rods 522, and can be used to drive the telescopic rods 522 to displace telescopically relative to the telescopic base 521. Since the telescopic drive module 523 can drive the telescopic rods 522 to expand and contract, which is a prior art and there are many ways, it will not be described in detail.

[0040] The microscope lens 524 is an electronic microscope lens. Each microscope lens 524 can be used for microscopic imaging and transmitting the captured microscopic image to a backend display device (not shown in the figure) for processing and display output.

[0041] In this embodiment, the field of view direction of the microscope lens 524 is obliquely downward and opposite along the second direction 802 towards the bottom end of the operating mechanism 6. However, in practice, in other embodiments of the present invention, the intersection angle of the field of view direction of the microscope lens 524 can be adjusted according to requirements. For example, it can be designed to intersect at 45°, 90° or other angles. In addition, the direction of the microscope lens 524 being displaced can also be changed by changing the telescopic displacement direction of the telescopic rod 522 relative to the telescopic base 521. Since there are various embodiments for the relative position of the field of view direction of the microscope lens 524, the implementation is not limited to the above embodiment.

[0042] Refer to Figure 3 、 7 FIG. 8, the operating mechanism 6 is installed on the rotating base 42 and can be driven to rotate by the rotating base 42. The operating mechanism 6 includes a second lifting and displacing unit 61 installed on the rotating base 42, a third lifting and displacing unit 62 installed on the second lifting and displacing unit 61, and an operating unit 63 installed on the third lifting and displacing unit 62.

[0043] The second lifting and displacing unit 61 includes a second base 611 installed and fixed on the rotating base 42 and extending vertically, a second lifting seat 612 displaceable vertically and connected to the third lifting and displacing unit 62, and a second lifting driver 613 installed on the second base 611 and connected to the second lifting seat 612. The second lifting driver 613 includes a second lifting drive motor 614 installed and fixed at the top of the second base 611, and a second transmission rack 615 installed on the second lifting seat 612 and connected to the second lifting drive motor 614. The second lifting drive motor 614 can be controlled to start, and drive the second lifting seat 612 to displace vertically relative to the second base 611 through the second transmission rack 615, thereby being used to adjust the length of the second lifting seat 612 protruding downward relative to the second base 611. In this embodiment, the second lifting drive motor 614 is a stepper motor, but the implementation is not limited to this.

[0044] The third lifting and shifting unit 62 includes a third base 621 fixedly mounted on the second lifting seat 612, a third lifting seat 622 displaceably mounted vertically beside the third base 621, and a third lifting drive 623 mounted on the third lifting seat 622 and connected to the third base 621. The third lifting drive 623 includes a third transmission rack 624 extending vertically and fixedly mounted on the third base 621, a third lifting drive motor 625 mounted in the third lifting seat 622, and a reduction gear set 626 mounted in the third lifting seat 622 and connected between the third lifting drive motor 625 and the third transmission rack 624.

[0045] The third lifting drive motor 625 can be controlled to operate, and drive the third lifting seat 622 to displace vertically relative to the third base 621 through the meshing action of the reduction gear set 626 and the third transmission rack 624.

[0046] In this embodiment, the pitch of the third transmission rack 624 is smaller than the pitch of the second transmission rack 615. That is to say, the lead of the third transmission rack 624 is different from that of the second transmission rack 615. The second lifting drive 613 can be used for large-scale lifting adjustment of the second lifting seat 612, and the third lifting drive 623 can be used for fine lifting and shifting of the third lifting seat 622.

[0047] The operating unit 63 can be a pipe fitting for connecting and assembling to a driving device (not shown in the figure), or an electronic device for signal connection to a driving device (not shown in the figure). The operating unit 63 is, for example but not limited to, a sampling needle, an injection needle, a laser instrument, an etching instrument, etc. The operating unit 63 can be linked and moved downward by the second lifting seat 612 to between the opposite sides of the microscope lens 524 and be located within the field of view of the microscope lens 524. And the operating unit 63 can be driven to perform operations such as suction, injection, etching, or punching on the biological sample 901 in the sample container 900. The injection is, for example but not limited to, injecting sperm, DNA, cell tissue, etc.

[0048] The imaging lens 7 is mounted on the bottom end surface of the third lifting seat 622 and can be used to image downward at the bottom end part of the operating unit 63.

[0049] Refer to Figure 1 、 2, when it is necessary to perform a predetermined operation on the biological sample 901 in the sample container 900 below with the micromanipulation device 200 of the present invention, the first rotator 315 and the second rotator 324 of the transfer mechanism 3 can be controlled to operate, so as to horizontally transfer the second transfer module 32 along the second direction 802, and horizontally transfer the second moving seat 323 along the first direction 801, and then drive the connecting seat mechanism 4 to drive the microscopic imaging mechanism 5 and the operating mechanism 6 to displace above the sample container 900.

[0050] Refer to Figure 2 , 6 , 9. Then, control and start the first lifting driver 513 to drive the first lifting transfer unit 51 to drive the microscopic imaging unit 52 to move downward, so that the sample container 900 is within the field of view of the microscopic lens 524. According to the imaging requirements, the telescopic drive module 523 can be controlled to transfer the microscopic lens 524 to adjust the field of view of the microscopic lens 524. When necessary, the rotation driver 43 can also be controlled to start to rotate and adjust the field of view of the microscopic lens 524.

[0051] When it is necessary to perform a predetermined operation on the biological sample 901 in the sample container 900, the second lifting transfer unit 61 can be controlled to start, so as to drive the second lifting seat 612 to drive the third lifting transfer unit 62 and the operating unit 63 to quickly move downward, so that the operating unit 63 is within the field of view of the microscopic lens 524, and then the position of the operating unit 63 relative to the biological sample 901 in the sample container 900 can be viewed from different angles.

[0052] Then, by controlling the operation of the third lifting driver 623 (shown in Figure 8 ) of the third lifting transfer unit 62, the vertical displacement of the operating unit 63 relative to the biological sample 901 in the sample container 900 can be finely adjusted. With the assistance of the microscopic image obtained by the microscopic lens 524, the user can more accurately control the operation of the third lifting driver 623, so that the operating unit 63 performs a predetermined treatment on a specific biological sample 901, such as sampling, injection, etching or drilling.

[0053] At the same time, in cooperation with the design that the imaging lens 7 can obtain the image of the bottom end of the operating unit 63, it can assist in confirming the relative position relationship between the operating unit 63 and the biological sample 901 to be processed, and assist in monitoring the operation process of the operating unit 63.

[0054] Cooperate with reference to Figure 1, In addition, the transfer mechanism 3 can be used to transfer the connection base mechanism 4 in the first direction 801 and the second direction 802. The design of synchronously horizontally transferring the microscopic imaging mechanism 5 and the operating mechanism 6 can facilitate arranging a plurality of the sample containers 900 each containing the biological sample 901 to be processed at intervals below the operating mechanism 6. Then, by horizontally transferring the connection base mechanism 4 through the transfer mechanism 3, the microscopic imaging mechanism 5 and the operating mechanism 6 are synchronously transferred, and can be used to perform predetermined operation processes on each of the sample containers 900 one by one.

[0055] In summary, through the structural design of the microscopic lens 524 of the microscopic imaging mechanism 5 and the structural design that the operating unit 63 of the operating mechanism 6 can be transferred into the field of view of the microscopic lens 524, when the micromanipulation device 200 of the present invention performs the operation process of the biological sample 901 through the operating unit 63, the relative positional relationship between the operating unit 63 and the biological sample 901 can be observed by using the microscopic lens 524 at different viewing angles, and sampling can be performed more precisely. Furthermore, through the structural design that the second lifting and transferring unit 61 and the third lifting and transferring unit 62 of the operating mechanism 6 have different lifting and transferring lead screws, they can be respectively used to lift and transfer the operating unit 63 significantly and quickly, and to lift and transfer the operating unit 63 slightly.

[0056] Furthermore, through the structural design that the connection base mechanism 4 can rotate the microscopic imaging mechanism 5 and the operating mechanism 6 relative to the sample container 900, it is convenient to select to perform various processes on the biological sample 901 in the sample container 900 from different tilting angles. In addition, through the structural design that the transfer mechanism 3 can transfer the microscopic imaging mechanism 5 and the operating mechanism 6 in the first direction 801 and the second direction 802, it is convenient to perform the processing operation of a large number of sample containers 900.

[0057] Moreover, through the structural design of the transfer mechanism 3, the connection base mechanism 4, the microscopic imaging mechanism 5 and the operating mechanism 6, it is also suitable for cooperating with automated equipment for intelligent control.

[0058] Therefore, the micromanipulation device 200 of the present invention is indeed a quite innovative, convenient and practical creation, and can indeed achieve the purpose of the present invention.

[0059] The above are only examples of the present invention, and the scope of implementation of the present invention cannot be limited thereby. All simple equivalent changes and modifications made according to the claims and the content of the specification of the present invention still belong to the scope of the present invention.

Claims

1. A micromanipulation device is applicable to perform microscopic imaging and predetermined operation processing on a biological sample in a sample container. The micromanipulation device includes a connection base mechanism, a microscopic imaging mechanism, and an operation mechanism, and is characterized in that: The microscopic imaging mechanism includes a first lifting and shifting unit installed on the connecting seat mechanism, and a microscopic imaging unit installed on the first lifting and shifting unit. The first lifting and shifting unit can be controlled to start and vertically shift the microscopic imaging unit relative to the sample container. The microscopic imaging unit includes two microscopic lenses spaced apart and with intersecting field-of-view directions. Each microscopic lens can be used to perform microscopic imaging on the biological sample in the sample container. The operating mechanism includes a second lifting and shifting unit installed on the connecting seat mechanism, and an operating unit capable of performing predetermined operation processing on the biological sample contained in the sample container. The second lifting and shifting unit can be controlled to start and vertically shift the operating unit relative to the sample container, and can be used to drive the operating unit to move down into the field of view of the microscopic lens and insert downward into the sample container.

2. The micromanipulation device according to claim 1, characterized in that: The microscopic imaging unit further includes a telescopic base installed on the first lifting and shifting unit, two telescopic rods installed on the telescopic base and protruding out of the telescopic base, and a telescopic drive module installed on the telescopic base and connected to the telescopic rods. The telescopic drive module can be controlled to start, and drive each telescopic rod to perform telescopic displacement relative to the telescopic base. The microscopic lenses are respectively installed on the telescopic rods and can be individually driven to displace by the telescopic displacement of the telescopic rods.

3. The micromanipulation device according to claim 1, wherein: The connecting seat mechanism includes a connecting seat, a rotating seat rotatably installed on the connecting seat along a horizontal axis, and a rotation driver installed on the connecting seat and connected to the rotating seat. The first lifting and shifting unit and the second lifting and shifting unit are installed on the rotating seat. The rotation driver can be controlled to start, and drive the rotating seat to drive the first lifting and shifting unit and the second lifting and shifting unit to rotate relative to the connecting seat.

4. The micromanipulation device according to claim 3, wherein: The microscopic operation device further includes a shifting mechanism connected to the connecting seat. The shifting mechanism includes two first shifting modules spaced parallel in a horizontal first direction, and a second shifting module horizontally extending across the first shifting modules in the first direction. Each first shifting module includes a first transmission screw and a first slide rail spaced parallel and extending in a second direction horizontally orthogonal to the first direction, a first moving seat displaceable along the second direction and sleeved on the first slide rail and screwed to the first transmission screw, and a first rotator connected to the first transmission screw. The first transmission screw can be rotated by the first rotator to drive the first moving seat to displace along the first slide rail. The second shifting module straddles the first moving seats and is connected to the connecting seat, and can be driven by the first moving seats to shift the connecting seat mechanism relative to the sample container.

5. The micromanipulation device according to claim 4, characterized in that: The second translation and displacement module includes a second transmission screw rod and a second slide rail that are spaced parallel and extend in the first direction and span between the first moving seats, a second moving seat that is sleeved on the second slide rail and can be displaced in the first direction and is screwed to the second transmission screw rod, and a second rotator that is installed on one of the first moving seats and is connected to the second transmission screw rod. The second moving seat is connected to the connecting seat. The second transmission screw rod can be rotated by the second rotator to drive the second moving seat to displace along the second slide rail.

6. The micromanipulation device according to claim 1, characterized in that: The first lifting and translation unit includes a first base installed and fixed on the connecting seat mechanism, a first lifting seat that can be displaced vertically and is installed on the first base, and a first lifting driver installed on the first base and connected to the first lifting seat. The microscopic imaging unit is installed on the first lifting seat. The first lifting seat can be driven by the first lifting driver to vertically displace the microscopic imaging unit relative to the first base.

7. The micromanipulation device according to claim 1, characterized in that: The operating mechanism further includes a third lifting and translation unit installed between the second lifting and translation unit and the operating unit. The second lifting and translation unit can be driven to vertically displace the operating unit through the third lifting and translation unit. The third lifting and translation unit can be driven to vertically displace the operating unit relative to the second lifting and translation unit. The second lifting and translation unit and the third lifting and translation unit are driven to have different leads for vertically displacing the operating unit.

8. The micromanipulation device according to claim 7, wherein: The second lifting and translation unit includes a second base installed on the connecting seat mechanism, a second lifting seat that can be displaced vertically and is installed on the second base, and a second lifting driver installed on one of the second base and the second lifting seat and connected to the other of them. The third lifting and translation unit is installed on the second lifting seat. The second lifting driver can be controlled to start and drive the second lifting seat to displace vertically relative to the second base, thereby vertically displacing the third lifting and translation unit and the operating unit.

9. The micromanipulation device according to claim 8, characterized in that: The second lifting driver includes a second transmission rack that extends vertically and is fixed on the second lifting seat, and a second lifting drive motor installed and fixed on the second base and meshed with the second transmission rack. The second lifting drive motor can be controlled to start and drive the second transmission rack to drive the second lifting seat to displace vertically relative to the second base.

10. The micromanipulation device according to claim 8, characterized in that: The third lifting and translation unit includes a third base installed on the second lifting seat, a third lifting seat that can be displaced vertically and is installed on the third base, and a third lifting driver installed on one of the third lifting seat and the third base and connected to the other of them. The third lifting driver can be controlled to start and drive the third lifting seat to displace vertically relative to the third base. The operating unit is installed on the third lifting seat.

11. The micromanipulation device according to claim 10, characterized in that: The third lifting driver includes a third transmission rack fixedly extending up and down on the third base, a third lifting drive motor mounted on the third lifting seat, and a reduction gear set mounted on the third lifting seat and connected between the third lifting drive motor and the third transmission rack.

12. The micromanipulation device according to claim 10, characterized in that: The micromanipulation device further includes an imaging lens mounted on the bottom end surface of the third lifting seat and capable of capturing an image downward toward the bottom end of the operating unit.

Images