Biosafety control method for cowpea diseases and insect pests based on screening and identification technology research

Abstract

The invention discloses a vigna unguiculata pest and disease damage biosafety prevention and control method based on screening and identification technology research. The method comprises the following steps: S1, identifying and selecting vigna unguiculata disease-resistant varieties; s2, testing and comparing the treatment effects of different disease-resistant agents on the cowpeas, and selecting the disease-resistant agent with the best effect; s3, treating the seeds before sowing; s4, soil insect prevention and control; s5, soil-borne diseases and root diseases are prevented and controlled; s6, preventing and treating leaf diseases; s7, preventing and controlling pests on the ground; s8, using an immunopotentiator; and S9, field cleaning. According to the method, disease-resistant varieties are identified and screened in the early stage of sowing, a new variety of root rot resistant cowpeas is bred and screened out, diseases are reduced from the source, and the prevention and treatment effects of biopesticide on main diseases and insect pests in different growth periods of cowpeas are systematically studied; the green comprehensive prevention and control technology with biopesticide as the main part and chemical pesticide synergistic prevention and control as the core is specifically used, economic and ecological benefits are considered, and green and safe production of cowpeas is guaranteed.

Description

Technical Field

[0001] The present invention belongs to the field of biological safety control of cowpea diseases and pests, and particularly relates to a biological safety control method for cowpea diseases and pests based on screening and identification technology research. Background Art

[0002] Cowpea is an important leguminous vegetable crop in Sichuan. It is the main raw material for pickled vegetable processing and also a major vegetable variety for farmers to increase their income. Due to the early low temperature and less sunshine, and high temperature and rainy in summer in the Sichuan Basin, cowpea is vulnerable to more than a dozen diseases and pests such as soil-borne diseases, leaf diseases, and insect pests from sowing to harvesting. Especially during the peak harvesting period, the damage of insect pests such as Maruca testulalis is serious.

[0003] And growers overuse and misuse pesticides to ensure the yield. The number of pesticide applications during the whole growth period exceeds 10 times. At the same time, in the control of cowpea diseases and pests, most of the existing technologies use some conventional chemical pesticides, and a small amount of physical and chemical trapping and control methods such as insect-proof nets, insecticidal lamps, and traps are applied. Most of these control measures have not passed the identification and screening and do not have a reliable practical theoretical basis. Therefore, they cannot fundamentally reduce the occurrence of diseases. While the control effect is not good, it will also lead to excessive pesticide residues, and the quality and safety of cowpea are seriously threatened. In order to promote the green and high-quality development of the cowpea industry, it is urgent to provide a biological safety control method for cowpea diseases and pests based on screening and identification technology research. Summary of the Invention

[0004] The purpose of the present invention is to provide a biological safety control method for cowpea diseases and pests based on screening and identification technology research to solve the problems mentioned in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A biological safety control method for cowpea diseases and pests based on screening and identification technology research, including the following steps:

[0006] S1: Identification and selection of disease-resistant cowpea varieties. The specific steps are as follows: Isolate and identify that the main pathogen of cowpea root rot in the Chengdu Plain is Fusarium oxysporum. Use this dominant strain to conduct seedling inoculation identification, field disease nursery identification and comprehensive evaluation of agronomic traits on 40 cowpea materials and varieties, and screen out Chengjiang 17, Chengjiang 30, and Chengjiang 6 as disease-resistant cowpea varieties for utilization;

[0007] S2: Comparative test on the treatment effect of different disease-resistant pesticides on cowpea, and select the best disease-resistant pesticide, specifically including:

[0008] 1) Compare the control effects of different pesticides on cowpea root rot and select the best control pesticide for cowpea root rot;

[0009] 2) Compare the effects of different pesticides on the prevention and control of cowpea leaf diseases, and select the best pesticide for the prevention and control of cowpea leaf diseases;

[0010] 3) Compare the control effects of different pesticides on cowpea pod borer and select the best pesticide for cowpea pod borer;

[0011] S3: Seed treatment before sowing: soak the seeds with 200 times dilution of biological agent Bacillus subtilis before sowing;

[0012] S4: Control of underground pests: For cowpea fields, 500g of Beauveria bassiana or 3kg of Metarhizium anisopliae is mixed with soil and fertilizer and applied. For plots with serious underground pests, 1kg / mu of chemical pesticide Thiamethoxam can be applied in combination, and the amount used can be reduced year by year;

[0013] S5: Prevention and control of soil-borne diseases and root diseases: Use 500 times dilution of Trichoderma harzianum for soil treatment by spraying on the furrow surface. After sowing or planting, use 4L of Bacillus polymyxa per mu for root irrigation 2-3 times. If the disease is serious, use 1000 times dilution of metalaxyl and 200 times dilution of biological agent Bacillus subtilis for root irrigation 2-3 times alternately.

[0014] S6: Leaf disease prevention and control: Before flowering and pod setting, use Bacillus subtilis and Bacillus amyloliquefaciens biological agents to prevent leaf diseases. When the disease is serious, use 1000 times of pyraclostrobin and 70% sulfur and manganese zinc chemical pesticides for prevention and control; during the flowering and pod setting period, alternately use 200 times of Bacillus amyloliquefaciens and 20 times of Bacillus subtilis to spray the leaves up and down, once every 7-10 days;

[0015] S7: Ground pest control: Color plates and insect-proof nets are used in facility cultivation, and insecticidal lamps and sex attractant traps are used in open-field contiguous bases to control ground pests. Before flowering and pod setting, biological pesticides such as 200 times of Beauveria bassiana, 500 times of Metarhizium anisopliae, and 500 times of Bacillus thuringiensis are used for alternate prevention. If the pests are serious, ethyl spinetoram, cyantraniliprole, and chlorantraniliprole can be used for control. During the flowering and pod setting period, 500 times of Metarhizium anisopliae and 1000 times of Bacillus thuringiensis are used for timely spraying in the morning when the flowers bloom, once every 5-7 days. If the pests are serious, low-toxic pesticides with a safe interval of no more than 3 days can be used;

[0016] S8: Use of immune enhancers: During the seedling stage, vine extension stage, flowering and podding stage, especially when encountering viral diseases, low temperature and high temperature climate changes, spray 1000 times diluted amino oligosaccharides or 500 times diluted S-inducer or 20 times diluted Kang No. 3 and other immune inducers + potassium dihydrogen phosphate foliar fertilizer to improve the resistance of plants and promote flower bud differentiation;

[0017] S9: Clean the fields: After the harvest, clean up the stumps, dead leaves, weeds, etc. in time to reduce the base number of diseases and insect pests in the fields.

[0018] Preferably, the specific method for comparing the control effects of treating with different agents on cowpea root rot is as follows: for cowpea root rot, the test variety is Chengjiang 30. The seedlings are transplanted for testing in multiple plots. Metalaxyl-M and hymexazol, the biological agents Bacillus subtilis and Bacillus polymyxa are used to irrigate the roots of cowpeas in different plots respectively. The disease is investigated by counting the diseased plants 15 days and 45 days after transplantation, and the yield and benefits are also counted. The test results show that the best application effect is achieved by irrigating the roots with 200-fold solution of Bacillus subtilis at the time of planting, and then irrigating with 1000-fold solution of hymexazol and Bacillus subtilis solution every 15 days for a total of 3 times in coordination.

[0019] Preferably, the specific method for comparing the control effects of treating with different agents on cowpea leaf diseases is as follows: for cowpea leaf diseases, the open-field cultivation is selected for the test, and the test variety is Chengjiang 17. The seedlings are transplanted for testing in multiple plots. The chemical pesticide pyraclostrobin, and the biological agents Bacillus subtilis and Bacillus amyloliquefaciens are used to investigate and apply pesticides when the cowpeas start to get sick. The incidence rate is counted before each application. The results show that the control effects of the chemical pesticide azoxystrobin and the biological agent on cowpea leaf diseases are quite the same.

[0020] Preferably, the comparison of the control effects of treating with different agents on cowpea pod borers is as follows: the test variety is Chengjiang 17. The plots with severe occurrence of cowpea pod borers in previous years are selected for the test. The seedlings are transplanted for testing in multiple plots. The chemical pesticide chlorantraniliprole, and the biological agents Metarhizium anisopliae and Bacillus thuringiensis are used to apply pesticides after the cowpeas are in budding stage. 50 cowpeas are picked from each plot for investigation and statistics of the pod borer rate and the control effect. The results show that the effective periods of the agents of Metarhizium anisopliae and Bacillus thuringiensis are longer, and the efficacy of the chemical pesticide chlorantraniliprole decays quickly. Bacillus thuringiensis is selected for the prevention and control of cowpea pod borers.

[0021] Preferably, the test treatment form for comparing the control effects of treating with different agents on cowpea root rot is as follows:

[0022]

[0023] Preferably, the test treatment form for comparing the control effects of treating with different agents on cowpea leaf diseases is as follows:

[0024]

[0025]

[0026] Preferably, the test treatment form for comparing the control effects of treating with different agents on cowpea pod borers is as follows:

[0027]

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] By identifying and screening disease-resistant varieties in the early sowing stage, the present invention has bred and selected a new variety of cowpea resistant to root rot, reducing the occurrence of diseases from the source and lowering the threshold for the use of biological pesticides;

[0030] Through the treatment tests of different disease-resistant agents on cowpeas, the optimal usage plans of biological agents and chemical pesticides for different pests and diseases of cowpeas have been selected. At the same time, the control effects of biological pesticides on the main pests and diseases above and below the ground (root rot, rust, powdery mildew, legume pod borer, etc.) at different growth stages of cowpeas have been systematically studied, indicating that biological pesticides such as Bacillus polymyxa, Bacillus subtilis, Metarhizium anisopliae, and Bacillus thuringiensis can be effectively used for the prevention and control of cowpea pests and diseases, reducing the usage amount of chemical pesticides;

[0031] According to the characteristics of different growth stages, a green integrated prevention and control technology with biological pesticides as the main component and chemical pesticides as the synergistic control core is used before and after flowering. This technology effectively controls cowpea pests and diseases, reduces the use of chemical pesticides, takes into account economic and ecological benefits, and ensures the green and safe production of cowpeas. Specific Embodiments

[0032] In order to enable those skilled in the art to better understand the technical solutions in the embodiments of the present invention, the technical solutions of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. It should be understood that these descriptions are merely exemplary and not intended to limit the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present invention.

[0033] In addition, in the following description, the descriptions of well-known structures and technologies are omitted to avoid unnecessarily confusing the concepts disclosed in the present invention.

[0034] The present invention provides the following technical solutions:

[0035] A biological safety control method for cowpea pests and diseases based on screening and identification technologies, comprising the following steps:

[0036] S1: Identification and selection of disease-resistant cowpea varieties. The specific steps are as follows: Isolate and identify the main cowpea root rot pathogen in the Chengdu Plain as Fusarium oxysporum. Use this dominant strain to conduct seedling inoculation identification, field disease nursery identification, and comprehensive evaluation of agronomic traits on 40 cowpea materials and varieties, and select Chengjiang 17, Chengjiang 30, and Chengjiang 6 as disease-resistant cowpea varieties for utilization;

[0037] S2: Comparative experiment on the treatment effects of different anti-disease agents on cowpeas, and select the anti-disease agent with the best effect, specifically including:

[0038] 1) Compare the control effects of different agents on cowpea root rot, and select the best control agent for cowpea root rot;

[0039] 2) Compare the control effects of different agents on cowpea leaf diseases, and select the best control agent for cowpea leaf diseases;

[0040] 3) Compare the control effects of different agents on cowpea pod borers, and select the best control agent for cowpea pod borers;

[0041] S3: Pre-sowing treatment of seeds: Soak the seeds with 200-fold liquid of the biological bacterium Bacillus subtilis before sowing;

[0042] S4: Control of underground pests: Apply 500 g of Beauveria bassiana or 3 kg of Metarhizium anisopliae per mu of cowpea field by mixing with soil and fertilizer. For plots with severe underground pests, 1 kg of thiamethoxam can be applied per mu in combination with chemical pesticides, and the usage amount should be gradually reduced year by year;

[0043] S5: Control of soil-borne diseases and root diseases: Select 500-fold liquid of Trichoderma harzianum for soil treatment by spraying on the ridges and furrows. After sowing or planting, apply 4 L of Paenibacillus polymyxa per mu and irrigate the roots 2-3 times; When the diseases are severe, 1000-fold of metalaxyl·hymexazol and 200-fold liquid of the biological bacterium Bacillus subtilis can be selected to alternately irrigate the roots 2-3 times;

[0044] S6: Control of leaf diseases: Before flowering and pod setting, use biological agents such as Bacillus subtilis and Bacillus amyloliquefaciens to prevent leaf diseases. When the diseases are severe, appropriately use 1000-fold of pyraclostrobin·azoxystrobin and 70% sulfur·manganese zinc chemical pesticides for control; During the flowering and pod setting period, alternately spray 200-fold liquid of Bacillus amyloliquefaciens and 20-fold liquid of Bacillus subtilis on the upper and lower surfaces of the leaves, once every 7-10 days;

[0045] S7: Control of aboveground pests: In protected cultivation, use color plates and insect-proof nets. In open-field contiguous bases, use a series of physical and chemical control methods such as insecticidal lamps and sex pheromone traps to control aboveground pests. Before flowering and pod setting, alternately use biological pesticides such as 200-fold of Beauveria bassiana, 500-fold of Metarhizium anisopliae, and 500-fold of Bacillus thuringiensis for prevention. If the pest infestation is severe, chemical agents such as spinetoram, cyantraniliprole, and chlorantraniliprole can be selected for control; During the flowering and pod setting period, use 500-fold of Metarhizium anisopliae and 1000-fold of Bacillus thuringiensis biological pesticides to spray in time when the flowers bloom in the morning, once every 5-7 days. When the pest infestation is severe, select pesticides with low toxicity and a safety interval of no more than 3 days;

[0046] S8: Use of immune enhancers: During the seedling stage, vine extension stage, flowering and pod setting stage, especially when encountering virus diseases, low temperature or high temperature, spray 1000 times diluted amino oligosaccharides or 500 times diluted S-inducer or 20 times diluted Kang No. 3 and other immune inducers + potassium dihydrogen phosphate foliar fertilizer to improve the resistance of plants and promote flower bud differentiation;

[0047] S9: Clean the fields: After the harvest, clean up the stumps, dead leaves, weeds, etc. in time to reduce the base number of pests and diseases in the fields.

[0048] Example 1, the specific method for comparing the control effects of different drug treatments on cowpea root rot is as follows: for cowpea root rot, the test variety is selected as Chengjiang 30, and a plurality of planting plots are tested in a seedling transplanting method, and metalaxyl·hypromex, biological agents Bacillus subtilis and Bacillus polymyxa are used to irrigate the roots of cowpeas in different planting plots, and the disease survey is carried out on diseased plants 15d and 45d after planting, and the yield and benefit are counted. The test results show that the best application effect is achieved by irrigating the roots with 200 times of Bacillus subtilis solution during planting, and irrigating the roots with 1000 times of hypromex and Bacillus subtilis solution every 15d, and using them synergistically for a total of 3 times. The test treatment table is as follows:

[0049]

[0050] The test results are shown in the following table:

[0051]

[0052] Example 2, the specific method for comparing the control effects of different pesticides on cowpea leaf diseases is as follows: for cowpea leaf diseases, the test selected open field cultivation, the test variety was Chengjiao 17, the seedling transplanting method was adopted, multiple planting plots were tested, and the chemical pesticide pyraclostrobin, the biological agent Bacillus subtilis and Bacillus amyloliquefaciens were used to investigate and apply the drugs when the cowpeas began to become ill, and the investigation was conducted before each application of the drugs, and the incidence rate was statistically calculated. The results showed that the chemical pesticide azoxystrobin and the biological agent had equivalent control effects on cowpea leaf diseases, and the test treatment table was as follows:

[0053]

[0054] The test results are shown in the following table:

[0055]

[0056]

[0057] Example 3. Comparison of the control effects of cowpea pod borers treated with different agents: The test variety was Chengjiang 17. Select fields with a relatively heavy incidence of pod borers over the years for the experiment. The experiment was carried out in multiple plots by transplanting seedlings. After the cowpeas showed flower buds, chemical pesticide chlorantraniliprole, biological agents Metarhizium anisopliae and Bacillus thuringiensis were applied. 50 cowpeas were picked from each plot for investigation and statistics of the pod borer rate and control effect. The results showed that the drug persistence periods of Metarhizium anisopliae and Bacillus thuringiensis were relatively long, and the efficacy of the chemical pesticide chlorantraniliprole decayed rapidly. Bacillus thuringiensis was selected for the prevention and control of pod borers. The experimental treatment table is as follows:

[0058]

[0059] The experimental results are as follows:

[0060]

[0061] Finally, it should also be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. "And / or" means that either one of the two can be selected, or both can be selected. Moreover, the term "including" is intended to cover non-exclusively, so that a process, method, article or terminal device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or terminal device. Without further limitations, an element defined by the statement "including one..." does not exclude the existence of additional identical elements in the process, method, article or terminal device including the element.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the embodiments of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention. Any changes or replacements that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

[0063] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological safety control method for cowpea pests and diseases based on screening and identification technology, characterized in that: It includes the following steps: S1: Identification and selection of cowpea disease-resistant varieties. The specific steps are as follows: Isolate and identify the main pathogen of cowpea root rot in the Chengdu Plain as Fusarium oxysporum. Use this dominant strain to conduct seedling inoculation identification, field disease nursery identification, and comprehensive evaluation of agronomic traits on 40 cowpea materials and varieties, and select Chengjiang 17, Chengjiang 30, and Chengjiang 6 as disease-resistant cowpea varieties for utilization; S2: Comparative experiment on the treatment effects of different disease-resistant agents on cowpeas, and select the disease-resistant agent with the best effect. Specifically, it includes: 1) Comparison of the control effects of different agents on cowpea root rot, and select the best control agent for cowpea root rot; 2) Comparison of the control effects of different agents on cowpea leaf diseases, and select the best control agent for cowpea leaf diseases; 3) Comparison of the control effects of different agents on cowpea pod borers, and select the best control agent for cowpea pod borers; S3: Pretreatment of seeds before sowing: Soak the seeds in a 200-fold solution of the biological bacterium Bacillus subtilis before sowing; S4: Control of underground pests: Apply 500 g of Beauveria bassiana or 3 kg of Metarhizium anisopliae per mu of cowpea field by mixing with soil and fertilizer, and in severely infested plots, 1 kg / acre of the chemical pesticide thiamethoxam can be applied synergistically, and the usage amount should be gradually reduced year by year; S5: Control of soil-borne diseases and root diseases: Select a 500-fold solution of Trichoderma harzianum for soil treatment by spraying on the ridges and furrows. After sowing or transplanting, apply 4 L of Paenibacillus polymyxa per mu for root irrigation 2 - 3 times; When the disease is severe, metalaxyl·hymexazol 1000-fold and a 200-fold solution of the biological bacterium Bacillus subtilis can be alternately used for root irrigation 2 - 3 times; S6: Control of leaf diseases: Before flowering and pod setting, use biological agents such as Bacillus subtilis and Bacillus amyloliquefaciens to prevent leaf diseases. When the disease is severe, appropriately use 1000-fold pyraclostrobin·azoxystrobin and 70% sulfur·manganese zinc chemical pesticides for control; During the flowering and pod setting period, alternately spray a 200-fold solution of Bacillus amyloliquefaciens and a 20-fold solution of Bacillus subtilis on the upper and lower surfaces of the leaves, once every 7 - 10 days; S7: Control of above-ground pests: Use color plates and insect-proof nets in protected cultivation, and apply a series of physical and chemical trapping and control methods such as insecticidal lamps and sex pheromone traps in open-field contiguous bases to control above-ground pests. Before flowering and pod setting, alternately use biological pesticides such as 200-fold Beauveria bassiana, 500-fold Metarhizium anisopliae, and 500-fold Bacillus thuringiensis for prevention. If the pest infestation is severe, chemical agents such as spinetoram, cyantraniliprole, and chlorantraniliprole can be selected for control; During the flowering and pod setting period, spray a 500-fold solution of Metarhizium anisopliae and a 1000-fold solution of Bacillus thuringiensis in time when the flowers bloom in the morning, once every 5 - 7 days. When the pest infestation is severe, select pesticides with low toxicity and a safety interval of no more than 3 days; S8: Use of immune enhancers: During the seedling stage, vine extension stage, and flowering and pod setting stage, especially when encountering severe changes in climate such as virus diseases, low temperature, and high temperature, spray a 1000-fold solution of oligosaccharins or a 500-fold solution of S-ABA or a 20-fold solution of immune inducer Kang 3 plus potassium dihydrogen phosphate foliar fertilizer to improve the resistance of plants and promote flower bud differentiation; S9: Clean the field: After harvesting, promptly clean up the residual plants, fallen leaves, and weeds to reduce the base number of field pests and diseases.

2. A biological safety control method for cowpea diseases and pests based on screening and identification technology according to claim 1, characterized in that: The specific method for comparing the control effects of different drug treatments on cowpea root rot is as follows: for cowpea root rot, Chengjiang 30 is selected as the test variety, and the seedling transplanting method is adopted for the test, and the cowpeas planted in different fields are irrigated with meconazole, biological bacteria agents Bacillus subtilis and Bacillus polymyxa, respectively, and the disease survey is conducted on diseased plants 15d and 45d after transplanting, and the yield and benefit are counted. The test results show that the best effect is achieved by using 200 times diluted Bacillus subtilis for root irrigation during transplanting, and irrigating the roots with 1000 times diluted meconazole and Bacillus subtilis solution every 15d, and using them synergistically for a total of 3 times.

3. A biological safety control method for cowpea pests and diseases based on screening and identification technology according to claim 1, characterized in that: The specific method for comparing the control effects of different pesticides on cowpea rust and powdery mildew leaf diseases is as follows: for cowpea leaf diseases, open field cultivation was selected for the experiment, the experimental variety was Chengjiao 17, seedling transplanting was adopted, multiple planting fields were tested, and chemical pesticide pyraclostrobin, biological microbial agents Bacillus subtilis and Bacillus amyloliquefaciens were used to conduct investigations and apply pesticides when the cowpea disease began to occur, and investigations were conducted before each application of pesticides to calculate the incidence rate. The results showed that the chemical pesticide pyraclostrobin and biological microbial agents had equivalent control effects on cowpea leaf diseases.

4. A biological safety control method for cowpea diseases and pests based on screening and identification technology according to claim 1, characterized in that: The control effects of different pesticides on cowpea pod borer were compared: the test variety was Chengjiang 17, and fields with serious pod borer occurrence in previous years were selected. Multiple plots were tested by seedling transplanting. Chemical pesticide chlorantraniliprole, biological agents Metarhizium anisopliae and Bacillus thuringiensis were applied after cowpea buds appeared. 50 cowpeas were picked from each plot to investigate and count the pod infestation rate and control effect. The results showed that Metarhizium anisopliae and Bacillus thuringiensis had a longer lasting effect, and the chemical pesticide chlorantraniliprole decayed quickly, so Bacillus thuringiensis was selected to control the pod borer.

5. A biological safety control method for cowpea pests and diseases based on screening and identification technology according to claim 2, characterized in that: The comparison of the control effect of different agents on cowpea root rot is shown in the following table:

6. A biological safety control method for cowpea pests and diseases based on screening and identification technology according to claim 3, characterized in that: The comparison of the control effects of different agents on cowpea leaf diseases is shown in the following table:

7. A biological safety control method for cowpea pests and diseases based on screening and identification technology according to claim 4, characterized in that: The control effects of different agents on cowpea pod borer are compared, and the experimental treatment table is as follows:

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