A traditional Chinese medicine composition for treating heart failure and a preparation method thereof

Abstract

The application discloses a traditional Chinese medicine composition for treating heart failure and a preparation method thereof, and relates to the field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from red ginseng, Coptis chinensis, trichosanthes peel, earthworm, Rhodiolae, Ganoderma lucidum spore powder, Radix Polygoni Multiflori, Prunella vulgaris, prepared licorice and cassia twig. The traditional Chinese medicinal materials are soaked and then decocted, the filtrate is collected, filtered, concentrated, and then the extract is obtained, and the Ganoderma lucidum spore powder is added to prepare the traditional Chinese medicine composition. The composition is prepared according to the compatibility principles of tonifying deficiency and reducing excess, and regulating cold and heat, and can accurately match the syndrome type of yangqi deficiency, phlegm-heat and blood stasis blocking and collateral damage and yin exhaustion, thereby significantly improving the ejection fraction preserved heart failure composite syndrome type of chronic heart failure.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating heart failure and a preparation method thereof. BACKGROUND

[0002] Chronic heart failure (CHF) is a cardiovascular disease caused by various reasons leading to myocardial damage, resulting in decreased filling capacity of the heart and weakened ejection function, and causing blood stasis in the pulmonary and systemic circulation.

[0003] The current conventional treatment method mainly includes drug therapy, such as cardiac drugs, diuretics and vasodilators. However, due to the need for long-term drug therapy, the disease is often accompanied by certain adverse reactions. In the theory of traditional Chinese medicine, CHF belongs to the category of "heart obstruction", "palpitation", "asthma", "phlegm and fluid retention", etc. In Huangdi Neijing, it is described as: "If the pulse obstruction is not cured and the evil is again affected, it is stored in the heart... Heart obstruction, pulse obstruction, heart drumming, sudden gasping and wheezing, dry throat and belching, and cold air up to fear". It can be seen that the main cause of the disease is heart qi deficiency, which hinders the circulation of meridians and forms blood stasis, showing phlegm and fluid retention, blood stasis and phlegm and fluid retention, which hinders the movement of qi, and the water and fluid retention consumes yang qi, which circulates repeatedly, and finally leads to heart qi failure. The chronic heart failure syndrome in the Guide to Diagnosis and Treatment of Common Diseases in Internal Medicine of Traditional Chinese Medicine and the Guide to Clinical Research of New Chinese Drugs is classified as: ① Heart and lung qi deficiency; ② Qi and yin deficiency; ③ Qi deficiency and blood stasis; ④ Heart and kidney yang deficiency; ⑤ Water and fluid retention. Traditional Chinese medicine prescriptions are mostly aimed at "qi deficiency and blood stasis" or "heart and kidney yang deficiency", and there are few reports on the treatment of common composite types of heart failure with preserved ejection fraction (HFpEF) (such as yang deficiency and phlegm and heat stasis, collateral damage and yin exhaustion).

[0004] CN119074883A, the invention name "A traditional Chinese medicine composition for treating heart failure with preserved ejection fraction and its application" discloses a traditional Chinese medicine composition composed of prepared rehmannia, deer horn glue, cassia, carbonized ginger, white mustard seed, ephedra, prepared pinellia, angelica, and raw licorice, and points out that the traditional Chinese medicine composition combines warming yang and tonifying blood, and removing phlegm and blood stasis, which can supplement yang deficiency, nourish blood, remove phlegm and blood stasis, and treat the root cause by warming the kidney, tonifying essence and assisting yang, and treat the symptoms by removing phlegm and dredging collaterals. However, it only indicates that the traditional Chinese medicine composition can treat heart failure with preserved ejection fraction of yang deficiency and phlegm and heat stasis, and the treatment effect of heart failure of collateral damage and yin exhaustion is not mentioned. SUMMARY

[0005] The present application provides a traditional Chinese medicine composition for treating heart failure and a preparation method thereof, aiming at the common composite types of heart failure with preserved ejection fraction (yang deficiency and phlegm and heat stasis, collateral damage and yin exhaustion).

[0006] The Chinese medicine composition for treating heart failure of the present application comprises, by weight fraction, 10-20 parts of red ginseng, 5-15 parts of Coptis chinensis, 10-15 parts of Gualoupi, 5-10 parts of Earthworm, 10-15 parts of Rhodiola, 5-10 parts of Ganoderma lucidum spore powder, 5-10 parts of Radix Polygoni Multiflori, 5-10 parts of Prunella vulgaris, 5-10 parts of Radix Glycyrrhizae and 5-10 parts of Ramulus Cinnamomi.

[0007] Further, the Chinese medicine composition comprises, by weight fraction, 15-20 parts of red ginseng, 10-15 parts of Coptis chinensis, 12-15 parts of Gualoupi, 5-9 parts of Earthworm, 12-15 parts of Rhodiola, 6-8 parts of Ganoderma lucidum spore powder, 5-9 parts of Radix Polygoni Multiflori, 5-9 parts of Prunella vulgaris, 5-6 parts of Radix Glycyrrhizae and 5-6 parts of Ramulus Cinnamomi.

[0008] Further, the Chinese medicine composition comprises, by weight fraction, 15 parts of red ginseng, 10 parts of Coptis chinensis, 12 parts of Gualoupi, 9 parts of Earthworm, 12 parts of Rhodiola, 6 parts of Ganoderma lucidum spore powder, 9 parts of Radix Polygoni Multiflori, 9 parts of Prunella vulgaris, 6 parts of Radix Glycyrrhizae and 6 parts of Ramulus Cinnamomi.

[0009] The preparation method of the Chinese medicine composition for treating heart failure of the present application comprises the following steps:

[0010] 1) 10-20 parts of red ginseng, 5-15 parts of Coptis chinensis, 10-15 parts of Gualoupi, 5-10 parts of Earthworm, 10-15 parts of Rhodiola, 5-10 parts of Radix Polygoni Multiflori, 5-10 parts of Prunella vulgaris, 5-10 parts of Radix Glycyrrhizae and 5-10 parts of Ramulus Cinnamomi are weighed and prepared for use.

[0011] 2) 8-10 times of water is added to the above Chinese medicinal materials, and after soaking, decoction is carried out. After boiling with strong fire, decoction is carried out with weak fire for 30-40 min, and the filtrate is collected. Then 6 times of water is added, and the second decoction is carried out for 20-30 min, and the second decoction filtrate is collected.

[0012] 3) The two filtrates are combined, filtered, concentrated, and the extract is obtained. 5-10 parts of Ganoderma lucidum spore powder is added and stirred uniformly to obtain the product.

[0013] Further, the relative density of the extract at 20℃ is 1.08-1.12.

[0014] Further, the Ganoderma lucidum spore powder is treated by low-temperature wall-breaking before being added, and the particle size is less than 10 μm.

[0015] Further, the Earthworm is dried at low temperature, crushed through an 80-mesh sieve and decocted.

[0016] The present application is aimed at the main symptoms of yangqi deficiency combined with phlegm-heat and blood stasis and collateral damage: shortness of breath and fatigue, palpitation (qi deficiency and blood stasis), secondary symptoms: chest tightness and excessive phlegm, tongue purple and yellow greasy (phlegm-heat and blood stasis), night sweats and dry mouth (yin deficiency and internal heat), pathogenesis: qi deficiency and blood stasis as the root, phlegm-heat blocking the lung and yin deficiency and internal heat as the tip, in line with the pathological characteristics of patients with chronic heart failure (HFpEF) combined with metabolic syndrome.

[0017] The mechanism of action of the composition of the present application:

[0018] The monarch drug: red ginseng has the effects of tonifying qi and promoting blood circulation, which targets the main symptoms of yangqi deficiency, improves heart function (main symptoms of shortness of breath and fatigue) by tonifying qi and consolidating collapse, and enhances myocardial contractility by tonifying primordial qi to improve cardiac output. Red ginseng saponins promote glucose utilization and enhance myocardial energy metabolism.

[0019] The ministerial drug: Huanglian (clearing heat and reducing phlegm) + Gualoupi (expanding chest and regulating qi); Huanglian clears heat and detoxifies (treats "phlegm-heat accumulation inside the body"), and Gualoupi expands chest and regulates qi (treats "phlegm blocking qi stagnation"), both of which are used together to improve the secondary symptoms of "phlegm-heat and blood stasis". Huanglian berberine reduces systemic inflammation; Gualoupi reduces pulmonary hypertension.

[0020] The assistant drug: Dilong, Hongjingtian, Heshouwu, Xiaakucao intervene through multiple targets; Dilong promotes blood circulation and unblocks collaterals: aimed at the pathogenesis of "stagnation", improves blood stasis (tongue purple and dark), and reduces blood viscosity through the activity of plasmin. Hongjingtian tonifies qi and resists hypoxia: enhances HIF-1 alpha expression and protects myocardial cells from hypoxic injury. Heshouwu nourishes the liver and kidney: tetrahydroxystilbene glucoside promotes mitochondrial autophagy and improves energy metabolism disorder. Xiaakucao clears liver heat and reduces phlegm: Xiaakucao reduces myocardial fibrosis. Lingzhisonpowder regulates immunity: relieves systemic inflammation (treats the source of "phlegm-heat").

[0021] The making drug: Guizhi warms yang + Zhigancao regulates; Guizhi pungent and warm yang (relieves yang deficiency and internal cold), Zhigancao regulates all drugs, balances the bitter and cold nature of Huanglian, forms a pattern of cold and heat, and is suitable for virtual and actual mixed syndromes.

[0022] The composition of the present application precisely matches the "yangqi deficiency, phlegm-heat and blood stasis combined with collateral damage" syndrome type through the compatibility principles of tonifying deficiency and reducing excess, and regulating cold and heat, which significantly improves the composite syndrome type of heart failure with preserved ejection fraction in chronic heart failure.

[0023] The traditional Chinese medicine composition in the present application improves the "yangqi deficiency combined with phlegm-heat and blood stasis, collateral damage" syndrome type through the compatibility principles of tonifying qi and warming yang, clearing heat and reducing phlegm, and nourishing yin and unblocking collaterals: energy metabolism repair: red ginseng + Huanglian synergistically improves glucose utilization; mitochondrial autophagy regulation: Heshouwu removes damaged mitochondria; anti-inflammatory and antioxidant: Huanglian + Lingzhisonpowder anti-inflammatory and antioxidant. DETAILED DESCRIPTION

[0024] In order to make the purposes, technical solutions and advantages of the embodiments of the present application more clearly understood, the spirit of the present application will be described in detail below. Any person skilled in the art can make changes and modifications to the technology taught by the present application without departing from the spirit and scope of the present application after understanding the embodiments of the present application.

[0025] The illustrative embodiments of the present application and the descriptions thereof are used to explain the present application, but not as a limitation of the present application.

[0026] Embodiment 1

[0027] A method for treating heart failure by a traditional Chinese medicine composition of the present embodiment:

[0028] 1) Take 15g of red ginseng, 10g of Coptis chinensis, 12g of Gualoupi, 9g of Earthworm, 12g of Rhodiola, 9g of Polygoni multiflori radix, 9g of Prunella vulgaris, 6g of Ramulus cinnamomi, and 6g of Fried licorice root, and weigh them according to the proportion, remove impurities and clean them thoroughly; dry the earthworm at low temperature and crush it through an 80-mesh sieve;

[0029] 2) Put the above-mentioned medicinal materials into a decoction container, add 8 times the amount of water, soak for 30 minutes at room temperature, and make the medicinal materials fully wet; first decoction: change to a slow fire after boiling with a strong fire, and decoct for 40 minutes, collect the filtrate; second decoction: add 6 times the amount of water again, decoct for 30 minutes, and collect the filtrate;

[0030] 3) Combine the two filtrates, filter them through a 200-mesh sieve to remove the dregs, concentrate them to obtain an extract (the relative density of the extract at 20℃ is 1.08-1.12), and low-temperature break the wall of Ganoderma lucidum spores to a particle size of less than 10μm before adding them to the extract, stir them evenly, divide them into portions, seal them, and store them in a cold storage.

[0031] Embodiment 2

[0032] Chronic heart failure with preserved ejection fraction (HFpEF) is often accompanied by complex syndromes: deficiency of yang qi combined with phlegm-heat and blood stasis, and damage to collaterals and exhaustion of yin. When patients with this syndrome are combined with metabolic syndrome and pulmonary hypertension, they often show complex symptoms such as shortness of breath and fatigue (deficiency of yang qi), edema of lower limbs (yang deficiency and water flooding), chest tightness and excessive phlegm (phlegm-heat blocking the lung), night sweats and dry mouth (yin deficiency and internal heat), etc.

[0033] The clinical trial of the present embodiment is as follows:

[0034] 1. Inclusion criteria

[0035] ① Age 40-75 years old, diagnosed with chronic heart failure (NYHA II-III grade)

[0036] ② TCM syndrome differentiation is in accordance with "yangqi deficiency with phlegm-heat and blood stasis, collateral damage and yexie syndrome": main symptoms are shortness of breath, fatigue, cold limbs, chest tightness, sputum, tongue purple moss, yellow and greasy, night sweats and dry mouth (reference "Guidelines for Diagnosis and Treatment of Chronic Heart Failure (2022)"), and exclude patients with acute infection, severe liver and kidney diseases and malignant tumors.

[0037] 2. Treatment method

[0038] Control group (n=15): Qili Qiangxin Capsules (take according to the instructions).

[0039] Experimental group (n=15): decoction prepared in Example 1 (1 dose per day, taken twice a day in the morning and evening).

[0040] Course of treatment: 12 weeks.

[0041] 3. Observation index

[0042] 1) TCM syndrome score: assess the improvement of shortness of breath, cold limbs, chest tightness, night sweats, etc. (0-3 point grading) according to "Guidelines for Clinical Research of New Drugs of Traditional Chinese Medicine":

[0043] (1) Shortness of breath and fatigue

[0044] 0 points: no shortness of breath after activity, daily activities are not limited;

[0045] 1 point: shortness of breath when walking fast or going upstairs, mild fatigue;

[0046] 2 points: shortness of breath when walking slowly on flat ground, obvious fatigue;

[0047] 3 points: still feel shortness of breath in a resting state, extreme fatigue.

[0048] (2) Cold limbs

[0049] 0 points: warm limbs, no chills;

[0050] 1 point: limbs cool after exposure to cold, dressing can relieve;

[0051] 2 points: persistent chills, need thick clothing for warmth;

[0052] 3 points: cold limbs, accompanied by pale complexion.

[0053] (3) Chest tightness and sputum

[0054] 0 points: no chest tightness, less sputum;

[0055] 1 point: occasional chest tightness, less sputum;

[0056] 2 points: frequent chest tightness, moderate sputum;

[0057] 3 points: persistent chest tightness, sputum is large and sticky.

[0058] (4) Tongue purple moss yellow greasy

[0059] 0 points: tongue pale red moss thin white;

[0060] 1 point: tongue slightly purple, moss thin yellow;

[0061] 2 points: tongue purple dark, moss yellow greasy;

[0062] 3 points: tongue purple dark with blood stasis, moss yellow thick greasy.

[0063] (5) Night sweats dry mouth

[0064] 0 points: no night sweats, dry mouth;

[0065] 1 point: occasional night sweats, mild dry mouth;

[0066] 2 points: frequent night sweats, need to change clothes;

[0067] 3 points: heavy sweating, with severe dry mouth.

[0068] 2) Heart function indicators: left ventricular ejection fraction (LVEF), 6-minute walk distance (6MWD).

[0069] 3) Serum markers: NT-proBNP, IL-6.

[0070] 4) Safety indicators: liver and kidney function, electrocardiogram, adverse reaction record.

[0071] 4、Results

[0072] 1) Improvement of TCM syndrome

[0073] Table 1 TCM syndromes

[0074]

[0075] Note: *P<0.05, **P<0.01.

[0076] Before treatment, the symptom distribution of the two groups was balanced, and there was no statistically significant difference in age, gender, and distribution of underlying diseases (hypertension, diabetes) between the two groups (p>0.05), which was comparable.

[0077] Improvement rate = pre-treatment score - post-treatment score / pre-treatment score x 100%.

[0078] From the above table analysis,

[0079] (1) Yang deficiency (shortness of breath, lack of strength, cold limbs)

[0080] Shortness of breath and lack of strength in the experimental group improved by 65%, while in the control group it improved by 40%; cold limbs in the experimental group improved by 60%, while in the control group it improved by 35%;

[0081] (2) Phlegm and blood stasis (chest tightness and excessive sputum, purple tongue with yellowish fur)

[0082] The improvement rate of chest tightness and excessive sputum in the experimental group was 75%, and that in the control group was 30%; the improvement rate of purple tongue with yellowish fur in the experimental group was 68%, and that in the control group was 32%.

[0083] (3) Collateral damage and yin depletion (night sweats and dry mouth)

[0084] The improvement rate of night sweats and dry mouth in the experimental group was 62%, and that in the control group was 25%.

[0085] 2) Heart function and serum markers

[0086] Table 2 Heart function and serum markers 3) Safety

[0087] There were no serious adverse reactions in both groups, and there were no abnormal changes in liver and kidney function and electrocardiogram.

[0088] Results analysis:

[0089] (1) Deficiency of yang qi (shortness of breath, fatigue, cold limbs)

[0090] Red ginseng + cassia twig + roasted licorice root synergistically warms yang and replenishes qi. Red ginseng: greatly replenishes primordial qi and improves myocardial energy metabolism. Red ginseng and cassia twig are combined, with red ginseng as the "monarch" to supplement qi and consolidate the foundation, and cassia twig as the "minister" to warm and unblock heart yang, both of which synergistically enhance cardiac output (LVEF in the experimental group increased from 47.9% to 56.4%, compared with an increase to 52.1% in the control group, with a more significant increase), relieve shortness of breath and fatigue, and improve exercise tolerance (6MWD in the experimental group increased by 29%, while that in the control group only increased by 16%). Cassia twig: pungent and warm to assist yang, with volatile oil components (such as cinnamyl aldehyde) to enhance microcirculation blood flow speed, and combined with roasted licorice root to warm yang and transform qi, offset the bitter-cold nature of goldthread, and form a pattern of regulating cold and heat. Roasted licorice root: harmonizes all medicines, prolongs the half-life of red ginseng and cassia twig, and enhances the overall warm yang effect.

[0091] (2) Phlegm and blood stasis (chest tightness and excessive sputum, purple tongue with yellowish fur)

[0092] Huanglian + Gualoupi + Dilong + Xijuxiao synergistically clear heat and phlegm, activate blood and resist fibrosis (experimental group NT-proBNP decreased by 66%, while the control group decreased by 33%). Huanglian: berberine inhibits the TLR4 / NF-κB pathway and reduces IL-6 (experimental group decreased by 43%, significantly more than the control group decreased by 21%). Huanglian and red ginseng improve energy metabolism together. Huanglian and Gualoupi are combined, with Huanglian clearing heat and detoxifying as "minister", and Gualoupi relieving chest and regulating qi as "assistant", to treat phlegm-heat obstructing the lung together. Gualoupi: improve pulmonary hypertension by reducing ET-1 levels, relieve chest tightness and excessive phlegm; Gualoupi and Dilong are combined, with Gualoupi resolving phlegm and relieving chest, and Dilong dissolving thrombus and dredging collaterals, to treat phlegm and blood stasis together. Dilong: improve blood viscosity by activating plasmin, relieve purple and dark spots on the tongue. Xijuxiao: ursolic acid inhibits TGF-β / Smad3 phosphorylation and reduces collagen I / III deposition.

[0093] (3) Collateral damage and yin exhaustion (night sweats and dry mouth)

[0094] Hongjingtian + Lingzhi Spore Powder + Heshouwu synergistically nourish yin and dredge collaterals, and resist oxidation. Heshouwu: Stilbene glycoside activates the SIRT1 / PINK1 pathway, promotes mitochondrial autophagy, and removes damaged mitochondria. Heshouwu and Hongjingtian are combined, with Heshouwu nourishing liver and kidney yin and Hongjingtian resisting hypoxia, to treat collateral damage and yin exhaustion together. Hongjingtian: protect myocardial cells from hypoxic injury and relieve night sweats. Lingzhi Spore Powder: resist oxidation and remove ROS.

[0095] Therefore, the traditional Chinese medicine composition has a significant therapeutic effect on the common composite syndrome type of chronic heart failure (HFpEF): yang deficiency combined with phlegm-heat and blood stasis, collateral damage and yin exhaustion.

[0096] Example 3

[0097] Mouse experiment

[0098] 1) Model: 8-10 week old C57BL / 6J male mice were used, and high-fat feed (containing 45% fat, 20% protein, and 35% carbohydrates) was fed from the 3rd day after TAC operation for 8-12 weeks.

[0099] 2) Grouping: control group (sham operation + normal diet + normal saline); model group (normal saline); experimental group: low dose (3g / kg), medium dose (6g / kg), and high dose (9g / kg) of the traditional Chinese medicine composition in Example 1; positive control group: Qili Qiangxin Capsules (1.2g / kg).

[0100] 3) Detection index: glucose clearance rate: intraperitoneal injection of glucose (2g / kg), tail vein blood glucose determination (0, 15, 30, 60min). Transmission electron microscopy observation of myocardial autophagosome number. Detection of LC3-II / I ratio. ELISA detection of serum TNF-α level, IL-6, detection of myocardial SOD2 expression.

[0101] 4) Results

[0102] Table 3 Energy metabolism repair

[0103]

[0104] The results of the above table show that the blood glucose clearance rate of the experimental group (medium dose) is increased to 72%, indicating that the traditional Chinese medicine composition has the function of energy metabolism repair.

[0105] Table 4 Mitophagy

[0106]

[0107] Note: **P<0.01.

[0108] The results of the above table show that the traditional Chinese medicine composition promotes mitophagy, and the LC3-II / I ratio of the medium dose experimental group is significantly increased to 1.1 from 0.3 of the model group. The ability to remove damaged mitochondria is significantly improved (the number of autophagosomes in the medium dose experimental group is increased by 4 times compared with the model group)

[0109] Table 5 Anti-inflammatory and antioxidant

[0110]

[0111] Note: **P<0.01.

[0112] The results of the above table show that the traditional Chinese medicine composition of Huanglian+lingzhi spore powder synergistically improves the anti-inflammatory and antioxidant capacity, and the TNF-α of the medium dose group is reduced by 46% compared with the model group, and the IL-6 is reduced by 40% compared with the model group. The SOD2 expression is increased by about 37%.

[0113] In summary, the traditional Chinese medicine composition in the present application improves energy metabolism, mitophagy, inflammation and oxidative stress through multi-target synergistic effect, and its curative effect is significantly better than that of the positive control group (Qili Qiangxin capsule).

Claims

1. A traditional Chinese medicine composition for treating heart failure, characterized in that... The traditional Chinese medicine composition comprises, by weight, 10-20 parts red ginseng, 5-15 parts coptis, 10-15 parts trichosanthes peel, 5-10 parts earthworm, 10-15 parts rhodiola rosea, 5-10 parts ganoderma lucidum spore powder, 5-10 parts polygonum multiflorum, 5-10 parts prunella vulgaris, 5-10 parts prepared licorice root, and 5-10 parts cinnamon twig.

2. The traditional Chinese medicine composition for treating heart failure according to claim 1, characterized in that... The traditional Chinese medicine composition comprises, by weight, 15-20 parts red ginseng, 10-15 parts coptis, 12-15 parts trichosanthes peel, 5-9 parts earthworm, 12-15 parts rhodiola, 6-8 parts ganoderma spore powder, 5-9 parts fleeceflower root, 5-9 parts prunella vulgaris, 5-6 parts prepared licorice root, and 5-6 parts cinnamon twig.

3. The traditional Chinese medicine composition for treating heart failure as described in claim 1, characterized in that... The traditional Chinese medicine composition consists of 15 parts red ginseng, 10 parts coptis, 12 parts trichosanthes peel, 9 parts earthworm, 12 parts rhodiola, 6 parts ganoderma spore powder, 9 parts fleeceflower root, 9 parts prunella vulgaris, 6 parts prepared licorice root, and 6 parts cinnamon twig by weight.

4. A method for preparing the traditional Chinese medicine composition for treating heart failure as described in claim 1, characterized in that... The preparation method of the traditional Chinese medicine composition is as follows: 1) Weigh out 10-20 parts red ginseng, 5-15 parts coptis, 10-15 parts trichosanthes peel, 5-10 parts earthworm, 10-15 parts rhodiola, 5-10 parts polygonum multiflorum, 5-10 parts prunella vulgaris, 5-10 parts prepared licorice root, and 5-10 parts cinnamon twig according to the weight proportions, remove impurities and set aside. 2) Add 8 to 10 times the amount of water to the above-mentioned Chinese medicinal materials, soak them, and then decoct them. Bring to a boil over high heat, then simmer over low heat for 30 to 40 minutes and collect the filtrate. Add 6 times the amount of water again and decoct for 20 to 30 minutes. Collect the filtrate from the second decoction. 3) Combine the two filtrates, filter, concentrate to obtain an extract, add 5-10 parts by weight of Ganoderma lucidum spore powder, stir evenly, and you have the final product.

5. The method for preparing a traditional Chinese medicine composition for treating heart failure according to claim 4, characterized in that... The relative density of the extract at 20°C is 1.08-1.

12.

6. The method for preparing a traditional Chinese medicine composition for treating heart failure according to claim 4, characterized in that... The Ganoderma lucidum spore powder was subjected to low-temperature cell wall disruption treatment to a particle size of less than 10 μm before being added.

7. The method for preparing a traditional Chinese medicine composition for treating heart failure according to claim 4, characterized in that... The earthworms mentioned above need to be dried at low temperature, crushed and passed through an 80-mesh sieve, and then boiled.

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