Traditional Chinese medicine essential oil for treating liver depression and qi stagnation type stress obesity and preparation method of traditional Chinese medicine essential oil

Abstract

The invention provides traditional Chinese medicine essential oil for treating liver depression and qi stagnation type stress obesity and a preparation method of the traditional Chinese medicine essential oil. Active ingredients of a traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 5-20 parts of saffron crocus, 5-20 parts of valerian roots, 5-20 parts of dracocephalum moldavica and 10-40 parts of orange peel. Active components of the traditional Chinese medicine composition are prepared from saffron crocus, valerian root, dracocephalum moldavica and orange peel in proper parts by weight. The saffron crocus has the effects of soothing liver-qi stagnation and promoting blood circulation to remove blood stasis and is used as a monarch Valerian root has the effects of soothing nerves, stabilizing mind, calming and sleeping; dracocephalum moldavica can sooth the liver, relieve depression and calm the And the two are ministerial drugs. And the orange peel is used as an adjuvant drug for strengthening spleen and stomach, regulating qi and relieving middle energizer. The active ingredients extracted from the four raw materials cooperate with one another and supplement one another, so that the traditional Chinese medicine composition has multiple effects of soothing liver-qi stagnation, soothing nerves, stabilizing mind, strengthening spleen and harmonizing stomach, can relieve symptoms of stress obesity from multiple aspects, and achieves the effect of overall regulation. The traditional Chinese medicine composition is simple in raw materials, high in safety and capable of being used for a long time.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine essential oil for treating stress obesity of liver qi stagnation type and a preparation method thereof. Background Art

[0002] Stress obesity is a metabolic disease caused by long-term psychological or physiological stress. In today's society, the incidence of stress obesity is increasing year by year, which is closely related to high work pressure, fast pace of life and unhealthy lifestyle in modern society. Research shows that the obesity risk of people in a long-term high-stress environment is about 30%-40% higher than that of the general population. And obesity, as the basis of metabolic diseases such as fatty liver, diabetes, hypertension and cardiovascular and cerebrovascular diseases, will pose a severe challenge to the social public health environment and cause a huge economic burden.

[0003] Since stress is one of the fundamental causes of stress obesity, effective stress reduction methods are the key to treating this disease. Currently, the main stress reduction strategies include cognitive behavioral therapy, meditation and relaxation training, etc. At the same time, behavioral interventions including diet and exercise intervention and emotion management are also the main treatment options for stress obesity. In some cases, such as when stress obesity is accompanied by severe weight gain or other health problems (such as diabetes, hypertension), weight loss drugs, insulin sensitizers, cortisol antagonists and antidepressants, etc. should be selected.

[0004] Since the prevention and treatment interventions for stress obesity need to be adhered to for a long time, comprehensive intervention strategies show good application prospects. Traditional Chinese medicine is a treasure of the motherland's medicine. Due to its systematicness and low toxicity and side effects, it is receiving more and more attention from domestic and foreign scholars in the prevention, treatment and intervention management of many chronic metabolic diseases including stress obesity. Developing effective drugs that can be used for a long time according to the symptoms of different stress obesity is of great significance for the prevention and treatment of stress obesity. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to provide a traditional Chinese medicine essential oil for treating stress obesity of liver qi stagnation type and a preparation method thereof.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions.

[0007] The first aspect of the present invention provides a traditional Chinese medicine composition for treating stress obesity, and the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 5-20 parts of saffron, 5-20 parts of valerian root, 5-20 parts of dracocephalum moldavica, and 10-40 parts of orange peel.

[0008] In some embodiments, the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 10-15 parts of saffron, 10-15 parts of valerian root, 10-15 parts of dracocephalum moldavica, and 20-30 parts of orange peel.

[0009] The second aspect of the present invention provides a preparation method of the traditional Chinese medicine composition as described above, comprising the following steps: taking each raw material according to the dosage, adding ethanol for extraction 1-3 times; combining the filtrates and removing the solvent to obtain the traditional Chinese medicine composition.

[0010] In some embodiments, the temperature of the extraction is 40°C - 55°C, and the time is 2h - 6h; and / or,

[0011] the volume fraction of the ethanol is 75% - 95%; and / or,

[0012] the ratio of the total weight of the raw materials to the ethanol is 1:(10 - 15) g / mL; and / or,

[0013] the method for removing the solvent is vacuum concentration; and / or,

[0014] the relative density of the traditional Chinese medicine composition is 0.85 - 1.00, preferably 0.90 - 0.95.

[0015] The third aspect of the present invention provides the application of the traditional Chinese medicine composition as described above in the preparation of a drug for treating stress-induced obesity.

[0016] In some embodiments, the stress-induced obesity is liver-qi stagnation type stress-induced obesity.

[0017] The fourth aspect of the present invention provides a drug for treating stress-induced obesity, and the active ingredients of the drug comprise the traditional Chinese medicine composition as described above.

[0018] In some embodiments, the dosage form of the drug is an external dosage form, preferably an essential oil, including external application essential oil, aromatherapy essential oil, etc.

[0019] In some embodiments, the essential oil is a base oil containing the traditional Chinese medicine composition, and the volume fraction of the traditional Chinese medicine composition in the essential oil is 1% - 5%, preferably 2% - 5%.

[0020] In some embodiments, the base oil includes at least one of sweet almond oil, safflower oil, coconut oil, linseed oil, and grape seed oil.

[0021] The fifth aspect of the present invention provides a preparation method of the drug as described above, which is characterized by comprising the following steps: adding the traditional Chinese medicine composition into the base oil and mixing evenly to obtain the drug.

[0022] Compared with the prior art, the present invention has the following beneficial effects.

[0023] Based on long-term clinical practice experience and a large number of studies, the inventors of the present invention proposed that liver qi stagnation and restlessness of the mind are a common clinical syndrome type of stress-induced obesity. At the same time, it was pointed out that the above-mentioned etiological factors and pathogenesis will affect each other during the disease progression, and comprehensive consideration is required in the prevention and treatment interventions. Further research obtained a traditional Chinese medicine composition that can be used to treat stress-induced obesity of the liver qi stagnation type. The active ingredients of the traditional Chinese medicine composition are prepared from saffron, valerian root, dracocephalum moldavica, and orange peel in appropriate weight parts. In the formula, saffron soothes the liver and relieves depression, promoting blood circulation to remove blood stasis, and serves as the monarch drug. Valerian root calms the mind and relieves uneasiness, sedating and helping to sleep; dracocephalum moldavica soothes the liver and relieves depression, calming the mind and relieving uneasiness; the two together serve as the ministerial drugs. Orange peel strengthens the spleen and harmonizes the stomach, regulating qi and widening the middle-jiao, and serves as the adjuvant drug. The active ingredients extracted from the four raw materials cooperate with each other and complement each other, having multiple effects of soothes the liver and relieves depression, calming the mind and relieving uneasiness, strengthening the spleen and harmonizing the stomach, and can relieve the symptoms of stress-induced obesity from multiple aspects, achieving the effect of overall regulation.

[0024] The raw materials of the traditional Chinese medicine composition of the present invention are simple, with high safety and can be used for a long time. Drugs containing the traditional Chinese medicine composition of the present invention, such as essential oils, have the advantages of convenient use and no side effects, can be better applied to the prevention and treatment interventions of stress-induced obesity, and can be used as a representative of traditional Chinese medicine aromatherapy for a long time, with good prospects for achievement transformation. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is the detection result of the body weight of each group of mice.

[0026] Figure 2 It is the detection result of the food intake of each group of mice.

[0027] Figure 3 It is the detection result of the contents of TG and TC in the serum of each group of mice.

[0028] Figure 4 It is the detection result of the blood glucose of each group of mice.

[0029] Figure 5 It is the detection result of the HE and Oil Red O staining of the liver of each group of mice.

[0030] Figure 6 It is the detection result of the 5-HT contents in the hypothalamus and serum of each group of mice. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] In the following embodiments of the present invention, the experimental methods without specific conditions noted are usually carried out according to conventional conditions or according to the conditions recommended by the manufacturers. All kinds of commonly used chemical reagents used in the embodiments are commercially available products.

[0032] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as commonly understood by one of ordinary skill in the technical field to which the present invention pertains. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0033] The terms "comprising" and "having" and any variations thereof in the present invention are intended to cover non-exclusive inclusion. For example, a process, method, apparatus, product or device that comprises a series of steps is not limited to the listed steps or modules, but optionally further includes steps not listed, or optionally further includes other steps inherent to these processes, methods, products or devices.

[0034] The "and / or" mentioned in the present invention describes the association relationship of associated objects and indicates that three relationships may exist. For example, A and / or B may represent three situations: A exists alone, A and B exist simultaneously, and B exists alone.

[0035] Some embodiments of the present invention relate to a traditional Chinese medicine composition for treating stress obesity, and the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 5-20 parts of saffron, 5-20 parts of valerian root, 5-20 parts of dracocephalum moldavica, and 10-40 parts of orange peel.

[0036] From the perspective of traditional Chinese medicine, stress-induced obesity is mostly related to liver qi stagnation, disharmony between the spleen and stomach, deficiency of the lungs and kidneys, phlegm coagulation and blood stasis, and restlessness of the mind. Among them, the heart governs the mind, and restlessness of the mind affects the quality of sleep and emotional stability, making it difficult to relieve stress; the liver is responsible for dredging and regulating qi, and emotional stress can lead to stagnation of liver qi and poor qi movement, affecting the function of the spleen and stomach; the spleen is the foundation of acquired constitution, and disharmony of the spleen and stomach functions and abnormal transportation and transformation may lead to increased appetite and obesity; the kidney is the foundation of congenital constitution, and the lungs and kidneys regulate qi movement and water metabolism up and down. Deficiency of the lungs and kidneys is likely to lead to phlegm coagulation and blood stasis and qi stagnation. The inventors of the present invention, combining long-term clinical experience and a large number of studies, proposed that liver qi stagnation and restlessness of the mind are a common clinical syndrome type of stress-induced obesity, and at the same time pointed out that the above-mentioned etiological factors and pathogenesis will affect each other during the disease progression process, and comprehensive consideration is needed in the prevention and treatment intervention. Further research obtained a traditional Chinese medicine composition that can be used to treat stress-induced obesity of liver qi stagnation type. The active ingredients of the traditional Chinese medicine composition are prepared from saffron, valerian root, dracocephalum moldavica, and orange peel in appropriate weight parts. In the formula, saffron has the effects of soothing the liver and relieving depression, promoting blood circulation and removing blood stasis. As the monarch drug, it can provide the main treatment direction for the whole formula, targeting the pathogenesis of liver qi stagnation in stress-induced obesity. Valerian root and dracocephalum moldavica are used as ministerial drugs together. Valerian root has the effects of calming the mind and sedating and helping sleep, and can relieve the anxiety and sleep problems brought by stress, assisting saffron to regulate emotions; dracocephalum moldavica has the effects of soothing the liver and relieving depression, calming the mind, and synergistically relieving emotional stress with the main drug, helping to stabilize the mind and promoting physical and mental health. Orange peel is used as an adjuvant drug. Orange peel has the effects of strengthening the spleen and regulating the stomach, regulating qi and relieving distension, and can improve the function of the spleen and stomach, promote qi movement, and enhance the conditioning effect of the whole formula. The active ingredients extracted from the four raw materials cooperate with each other and complement each other, having multiple effects of soothing the liver and relieving depression, calming the mind, strengthening the spleen and regulating the stomach, and can relieve the symptoms of stress-induced obesity from multiple aspects, achieving the effect of overall regulation.

[0037] The traditional Chinese medicine composition of the present invention can regulate the content of neurotransmitters such as 5-hydroxytryptamine (5-HT) in the brain and then participate in the regulation process of various physiological functions such as emotion, sleep, and eating, and can effectively reduce the body weight of stress-induced obesity model mice.

[0038] In some embodiments of the present invention, a preparation method of the above-mentioned traditional Chinese medicine composition is involved, including the following steps: taking each raw material according to the dosage, adding ethanol for extraction 1 to 3 times; combining the filtrates, removing the solvent, to obtain the traditional Chinese medicine composition.

[0039] In some embodiments, the extraction temperature is 40°C to 55°C, and the time is 2h to 6h.

[0040] In some embodiments, the volume fraction of the ethanol is 75% to 95%.

[0041] In some embodiments, the liquid ratio of the total weight of the raw materials to the amount of ethanol is 1:(10-15) g / mL.

[0042] In some embodiments, the method for removing the solvent is the reduced pressure concentration method.

[0043] In some embodiments, the conditions of the reduced pressure concentration method are as follows: the pressure is 10 mmHg - 30 mmHg, the heating temperature is 30°C - 50°C, the rotation speed is 100 rpm - 200 rpm, and the evaporation time is 1 h - 3 h.

[0044] In some embodiments, the conditions of the reduced pressure concentration method are as follows: the pressure is 15 mmHg - 25 mmHg, the heating temperature is 35°C - 45°C, the rotation speed is 130 rpm - 180 rpm, and the evaporation time is 1.5 h - 2 h.

[0045] In some embodiments, the relative density of the traditional Chinese medicine composition is 0.85 - 1.00, preferably 0.90 - 0.95.

[0046] In some embodiments of the present invention, there is provided a drug for treating stress obesity, and the active ingredient of the drug comprises the traditional Chinese medicine composition described above.

[0047] The traditional Chinese medicine composition of the present invention can be made into a traditional Chinese medicine preparation for treating stress obesity with conventional excipients in the art, preferably an external preparation. For example, an appropriate amount of the traditional Chinese medicine composition is added to a base oil to make an essential oil for treating stress obesity, including external application essential oil, aromatherapy essential oil, etc.

[0048] In some embodiments, the volume fraction of the traditional Chinese medicine composition in the essential oil is 1% - 5%, including but not limited to 1%, 2%, 3%, 4%, 5%, or the range composed of any two of them.

[0049] In some embodiments, the base oil is at least one of sweet almond oil, safflower oil, coconut oil, linseed oil, and grape seed oil.

[0050] The following is an explanation in combination with specific embodiments.

[0051] Example 1

[0052] This example provides a traditional Chinese medicine composition for treating stress obesity, and the active ingredient of the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 parts of saffron, 10 parts of valerian root, 10 parts of dracocephalum moldavica, and 20 parts of orange peel.

[0053] The traditional Chinese medicine composition is prepared by the following method: Weigh saffron, valerian root, dracocephalum moldavica, and orange peel according to parts by weight. Use a mortar and pestle to grind the samples into fine powders to increase the extraction efficiency. Place the ground plant powders in a glass beaker, and add 85% ethanol with a volume 10 times that of the total weight of the raw materials to completely cover the samples. Heat in a water bath to 45°C and stir for extraction for about 3 hours. Filter the extract using filter paper and a glass funnel to remove the solid residue, and collect the filtrate in a clean beaker. Transfer the filtrate to a rotary evaporator and evaporate the solvent under reduced pressure as follows: the pressure is 20 mmHg, the heating temperature is 40°C, the rotation speed is 150 rpm, and evaporate for about 1.5 - 2 hours until a concentrated extract is obtained, which has an obvious aroma and color, a relative density of 0.90, and a refractive index of 1.45, to obtain the traditional Chinese medicine composition.

[0054] Example 2

[0055] In this example, taking the traditional Chinese medicine composition in Example 1 as an example, the therapeutic effect of the traditional Chinese medicine composition of the present invention on stress-induced obesity was studied.

[0056] 1. Experimental method

[0057] Using sweet almond oil as the base oil, add the traditional Chinese medicine composition described in Example 1 to sweet almond oil according to the volume fractions of the traditional Chinese medicine composition being 1%, 2%, and 3% respectively, and mix evenly to obtain essential oils with the volume fractions of the traditional Chinese medicine composition being 1%, 2%, and 3% respectively, for the study of the therapeutic effect on stress-induced obesity.

[0058] Use a high-fat, high-cholesterol, high-fructose diet to feed and a restricted stress environment to construct a mouse model of stress-induced obesity with liver qi stagnation. Set up a normal control group, a negative control group, and a drug essential oil treatment group. Specifically as follows:

[0059] Select male C57BL / 6 mice at 6 - 8 weeks old. After one week of adaptive feeding, start the experiment. The mice are given a high-fat, high-cholesterol, high-fructose diet (XT310, Synergistic Biology), given in sufficient amounts, allowed to eat freely without restricting the food intake, and the mice are randomly divided into 7 groups, with 6 mice in each group, and are treated as follows:

[0060] Group 1: Apply about 1 drop of the base oil to the back at an appropriate time (synchronized with the subsequent groups) as a normal control.

[0061] Group 2: Give a restricted stress environment (randomly select three days a week to place the mice in a mouse fixator for 30 minutes), and apply about 1 drop of the base oil to the back after each time the restricted stress environment is given, as a negative control.

[0062] The medicated essential oil treatment groups included groups 3 to 7. Each group of mice was given the same restricted stress environment as in group 2, and about 1 drop of medicated essential oil was applied to the back each time after the restricted stress environment was given. The medicated essential oils used in groups 3 to 7 were respectively:

[0063] Group 3: The above-mentioned medicated essential oil with a volume concentration of 1%.

[0064] Group 4: The above-mentioned medicated essential oil with a volume concentration of 2% was applied.

[0065] Group 5: The above-mentioned medicated essential oil with a volume concentration of 3% was applied.

[0066] Group 6: The control medicated essential oil with a volume concentration of 3%. The active ingredient traditional Chinese medicine composition of the control medicated essential oil was the same as that in Example 1 except that the Dracocephalum moldavica in the original formula was replaced with Mentha haplocalyx with similar efficacy in equal amount, and the control traditional Chinese medicine composition was obtained by the same method as in Example 1. Then, according to the volume fraction of the control traditional Chinese medicine composition being 3%, it was added to sweet almond oil and mixed evenly to obtain the control medicated essential oil.

[0067] Group 7: The control medicated essential oil with a volume concentration of 3%. The active ingredient traditional Chinese medicine composition of the control medicated essential oil was the same as that in Example 1 except that the Valeriana officinalis in the original formula was replaced with Albizia julibrissin Durazz. with similar efficacy in equal amount, and the control traditional Chinese medicine composition was obtained by the same method as in Example 1. Then, according to the volume fraction of the control traditional Chinese medicine composition being 3%, it was added to sweet almond oil and mixed evenly to obtain the control medicated essential oil.

[0068] The mice were allowed to eat and drink freely and were raised for 12 weeks. The mental state and activity of the mice were observed regularly, and the body weight and food intake were recorded weekly; at the end of the experiment, the body weight and food intake of each group of mice were recorded, the fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC) and 5-HT content were detected, and the liver tissues of the mice were stained with hematoxylin-eosin (HE) and oil red O. Among them, the body weight and food intake of the mice were directly recorded, the blood glucose was directly detected by a blood glucose meter, and TG and TC were directly detected by an automatic biochemical analyzer.

[0069] The detection method of 5-HT content was as follows: The mouse serum and brain tissues were detected by enzyme-linked immunosorbent assay (ELISA, BY-JZF0046, the 13th department). The samples were treated with pre-cooled PBS or special lysis buffer, and protein interference was removed by centrifugal ultrafiltration or acetonitrile precipitation method; the ELISA kit coated with specific 5-HT antibody was used for detection, and the absorbance at 450 nm was measured after incubating the samples and developing color according to the instructions. A standard curve was made synchronously, and the operation was carried out on ice throughout to prevent degradation. Each group was set with 6 replicates to ensure the accuracy of the data.

[0070] The HE staining method is as follows: Take out the liver tissue sample from the formalin fixative and continuously rinse it with running water for 4 h to remove the residual fixative and impurities. Subsequently, place the tissue sample in an automatic dehydrator for dehydration. After dehydration is completed, perform paraffin embedding in an embedding machine to make a paraffin block. Use a microtome to cut the paraffin block into sections with a thickness of 5 μm and attach the sections to glass slides. Immerse the dried sections in xylene for 10 min successively and repeat once to completely remove the paraffin. Then immerse them in absolute ethanol for 5 min successively and repeat the immersion for 2 min. Subsequently, immerse them in 95%, 90%, 80%, and 70% ethanol for 2 min each. Finally, rinse with distilled water and then immerse in PBS solution 3 times, 5 min each time, to ensure that the tissue is fully hydrated and impurities are removed. Immerse the sections in hematoxylin staining solution for 1 min to stain the cell nuclei. Rinse the sections with distilled water to remove the excess staining solution. Then place the sections in 0.4% hydrochloric acid alcohol for differentiation for 4 s, rinse the sections with distilled water again, and finally place the sections in 0.6% ammonia water for bluing treatment to make the cell nuclei more clearly stained. Finally, rinse the sections with running water. Immerse the sections in eosin staining solution for 2 min to stain the cytoplasm. Immerse the stained sections in 95% ethanol for 5 min successively, then in absolute ethanol for 5 min, and finally in xylene for 5 min to completely dehydrate the sections. After the sections are air-dried, drop neutral resin for mounting and take pictures.

[0071] The Oil Red O staining method is as follows: Pre-cool the cryostat in advance to ensure that the machine temperature is stable at an appropriate low temperature state. Take out the frozen tissue sample. After the sample temperature is balanced, place it on ice to melt and prepare for subsequent sectioning operations. Adhere the melted tissue sample to the cryostat stage with OCT glue to ensure that the tissue sample is firmly fixed. Place the stage with the adhered sample on the pre-cooled cryostat. After the OCT glue freezes and solidifies, section at a thickness of 8 μm. Gently adhere the sections with pre-labeled glass slides to ensure that the sections are firmly attached to the glass slides. Take out the frozen sections and rinse them in a container containing 60% isopropanol for 20 s to remove the impurities and residual OCT glue on the section surface. Mix Oil Red O dye and diluent in a ratio of 5:2 and filter it twice with filter paper to ensure that the staining solution is uniform and free of impurities. Immerse the sections in Oil Red O staining solution for 10 min to fully stain the lipid droplets. After staining is completed, rinse the sections with 60% isopropanol for 20 s to remove the excess Oil Red O staining solution, and then wash the sections with pre-warmed double-distilled water at 37 °C to ensure that the section surface is clean. Use hematoxylin (counterstain) to counterstain the sections for 4 min to stain the cell nuclei. Mount the sections with water-based mounting medium pre-warmed to a liquid state at 60 °C and take pictures.

[0072] The statistical analysis method is as follows: GraphPad Prism 7.0 software was used to process the data, and the data were expressed as mean ± standard deviation. One-way analysis of variance was used for between-group comparison of indicators, and analysis of variance for repeated measures was used for comparison of repeated measurement data. P<0.05 indicated significant difference.

[0073] 2. Experimental results

[0074] (1) Body weight of mice

[0075] As Figure 1 shown, the body weights of mice in all groups increased rapidly. Compared with the mice in group 1, the body weight growth rate of the mice in group 2 was significantly faster, and the difference between the two groups was statistically significant (P<0.05), indicating that the stress environment led to a further significant increase in the body weight of mice. Activity restriction is a common modeling method for stress, and at the same time, observing the general situation, the mice in group 2 showed phenomena such as irritability, increased frequency of mutual attack and biting, and obvious touch stress response. Combining with the body weight, it can be considered that the mouse model of stress-induced obesity with liver qi stagnation was successfully established.

[0076] Compared with group 2, the body weight growth rates of the mice treated with medicinal essential oils in groups 3-7 were relatively slowed down, but only the body weight differences of groups 3, 4, and 5 compared with that of group 2 were statistically significant (P<0.05), indicating that the medicinal essential oil of the present invention can better reduce the body weight of mice with stress-induced obesity of liver qi stagnation type.

[0077] In group 5, the essential oil of the present invention was used for treatment. In group 6, the dracocephalum moldavica in the active ingredient traditional Chinese medicine composition of the treatment essential oil was replaced with mint with similar efficacy in equal amount. In group 7, the valerian root in the active ingredient traditional Chinese medicine composition of the treatment essential oil was replaced with albizia bark with similar efficacy in equal amount. From Figure 1 this, it can be seen that compared with group 5, the body weight growth rates of the mice in groups 6 and 7 were relatively faster and the body weights were heavier, and the differences were statistically significant (P<0.05). The formula compatibility of the traditional Chinese medicine composition of the present invention is reasonable. The active ingredients extracted from the four raw materials of saffron, valerian root, dracocephalum moldavica, and orange peel cooperate with each other and complement each other, and have multiple effects of soothing the liver and relieving depression, calming the mind, and strengthening the spleen and regulating the stomach, and can relieve the symptoms of stress-induced obesity from multiple aspects and achieve the effect of overall regulation. Replacing one of the raw materials in the formula with other raw materials with similar efficacy (groups 6 and 7), the raw materials cannot cooperate well, resulting in a decline in curative effect. In particular, the combination of saffron and dracocephalum moldavica is particularly important for the efficacy of the traditional Chinese medicine composition of the present invention. After replacing dracocephalum moldavica with mint (group 6), the intervention efficacy of essential oil treatment on the increase of mouse body weight decreased most significantly.

[0078] (2) Food intake of mice

[0079] As Figure 2As shown, it can be seen that the food intake of mice in all embodiments increased slowly synchronously with body weight. Compared with the mice in Group 1, the growth rate of food intake in Group 2 mice was significantly faster, and the difference between the two groups was statistically significant (P < 0.05), indicating that the stress environment led to an increase in the food intake of mice, which is also a typical manifestation of stress-induced obesity. Compared with Group 2, the growth rate of food intake in mice in Groups 3-7 was relatively slower. Among them, only the food intake differences in Groups 3, 4, and 5 compared with Group 2 were statistically significant (P < 0.05), indicating that the medicinal essential oil of the present invention can effectively reduce stress eating. Among the treatments with each medicinal essential oil, the growth rate of food intake in Group 5 mice was the slowest. Compared with Group 5, the growth rate of food intake in Groups 6 and 7 was relatively faster, and the difference was statistically significant (P < 0.05). Among the three groups, the growth rate of food intake in Group 6 was the fastest.

[0080] (3) Mouse blood lipids

[0081] As Figure 3 shown, compared with Group 1, the contents of serum triglyceride (TG) and total cholesterol (TC) in Group 2 mice increased, and the difference was statistically significant (P < 0.05). Compared with Group 2, the contents of serum TG and TC in mice in Groups 3-7 decreased. Among them, only the TG content differences in Groups 4 and 5 compared with Group 2 were statistically significant (P < 0.05), and only the TC content differences in Groups 3, 4, and 5 compared with Group 2 were statistically significant (P < 0.05), indicating that the medicinal essential oil of the present invention is more conducive to improving the blood lipids of stress-induced obesity mice with liver qi stagnation type and assisting in the treatment of stress-induced obesity. At the same time, compared with Group 5, the blood lipid contents of mice in Groups 6 and 7 were relatively higher. Among them, the difference in serum TG content between Groups 6 and 7 compared with Group 5 was statistically significant (P < 0.05), and the difference in serum TC content between Group 6 and Group 5 was statistically significant (P < 0.05).

[0082] (4) Mouse blood glucose

[0083] As Figure 4 shown, compared with Group 1, the content of blood glucose (FBG) in Group 2 mice increased, and the difference was statistically significant (P < 0.05). Compared with Group 2, the serum blood glucose contents of mice in Groups 3-7 decreased. Among them, only the blood glucose differences in Groups 3, 4, and 5 compared with Group 2 mice were statistically significant (P < 0.05), indicating that the medicinal essential oil of the present invention is more conducive to improving the blood glucose of stress-induced obesity mice with liver qi stagnation type and assisting in the treatment of stress-induced obesity. At the same time, compared with Group 5, the blood glucose contents of mice in Groups 6 and 7 were relatively higher, and the difference between Group 6 and Group 5 was statistically significant (P < 0.05).

[0084] (5) Mouse liver steatosis and inflammatory response

[0085] AsFigure 5 As shown, obvious lipid droplet accumulation and inflammatory reactions occurred in the liver tissues of each group of mice. Compared with Group 1, fatty degeneration and inflammatory reactions were more obvious in the liver tissues of Group 2 mice. Compared with Group 2, the conditions of fatty degeneration and inflammatory reactions in the liver tissues of Group 3 - 7 mice were all improved, among which, the improvement in Group 5 was the most obvious, indicating that the medicinal essential oil of the present invention is more conducive to improving fatty liver in stress-induced obesity mice with liver qi stagnation type and assisting in the treatment of stress-induced obesity.

[0086] (6) 5-HT content in mice

[0087] As Figure 6 shown, compared with Group 1, the contents of 5-HT in the hypothalamus tissues and sera of Group 2 mice were both decreased, and the differences were statistically significant (P < 0.05). Compared with Group 2, the contents of 5-HT in the hypothalamus tissues and sera of Group 3 - 7 mice were both increased, but only in Group 3, Group 4, and Group 5, the differences in the contents of GABA in the hypothalamus tissues and sera compared with Group 2 mice were statistically significant (P < 0.05), and the increase in Group 5 was the most obvious. It shows that the medicinal essential oil of the present invention can better regulate the content of 5-HT in the hypothalamus tissues and sera of stress-induced obesity mice with liver qi stagnation type. At the same time, compared with Group 5, the contents of 5-HT in the hypothalamus tissues and sera of Group 6 and Group 7 mice decreased relatively, and the difference between Group 6 and Group 5 was statistically significant (P < 0.05).

[0088] In summary, the traditional Chinese medicine composition of the present invention can effectively regulate the blood glucose, blood lipids, and 5-HT content in stress-induced obesity mice with liver qi stagnation type, reduce the food intake and body weight of mice, and has an obvious improvement effect on stress-induced obesity with liver qi stagnation type.

[0089] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0090] The above-described embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent of the present invention should be subject to the appended claims.

Claims

1. A traditional Chinese medicine composition for treating stress obesity, characterized in that, The active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 5-20 parts of saffron, 5-20 parts of valerian root, 5-20 parts of Dracocephalum moldavica, and 10-40 parts of orange peel.

2. The traditional Chinese medicine composition according to claim 1, wherein, The active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 10-15 parts of saffron, 10-15 parts of valerian root, 10-15 parts of Dracocephalum moldavica, and 20-30 parts of orange peel.

3. The preparation method of the traditional Chinese medicine composition according to any one of claims 1 to 2, characterized in that It includes the following steps: Take each raw material according to the dosage, and add ethanol for extraction 1-3 times; Combine the filtrates, remove the solvent, and obtain the traditional Chinese medicine composition.

4. The preparation method according to claim 3, characterized in that, The temperature of the extraction is 40°C-55°C, and the time is 2h-6h; and / or, The volume fraction of the ethanol is 75%-95%; and / or, The ratio of the total weight of the raw materials to the ethanol is 1:(10-15) g / mL; and / or, The method for removing the solvent is the vacuum concentration method; and / or, The relative density of the traditional Chinese medicine composition is 0.85-1.

00.

5. Use of the traditional Chinese medicine composition according to any one of claims 1-2 in the preparation of a drug for treating stress obesity.

6. A drug for treating stress obesity, characterized in that, The active ingredients of the drug comprise the traditional Chinese medicine composition according to any one of claims 1-2.

7. The drug according to claim 6, characterized in that, The dosage form of the drug is an external dosage form, preferably an essential oil.

8. The medicament according to claim 7, wherein The essential oil is a base oil containing the traditional Chinese medicine composition, and the volume fraction of the traditional Chinese medicine composition in the essential oil is 1%-5%.

9. The drug according to claim 8, wherein The base oil includes at least one of sweet almond oil, safflower oil, coconut oil, linseed oil, and grape seed oil.

10. The preparation method of the drug according to claim 8 or 9, characterized in that, It includes the following steps: Add the traditional Chinese medicine composition to the base oil and mix evenly to obtain it.

Images