Method for preparing microbial soil conditioner by synergistically utilizing ardealite and ardealite leachate as well as product and application of microbial soil conditioner

Abstract

The invention discloses a method for preparing a microbial soil conditioner by synergistically utilizing ardealite and ardealite leachate as well as a product and application of the microbial soil conditioner. The method comprises the following steps: mixing the ardealite leachate and white mud paste, drying, activating at high temperature, adding sulfide slag and fly ash, granulating and aging to obtain a to-be-stabilized material; the method comprises the following steps: mixing papermaking material preparation waste residues, mushroom residues and a to-be-stabilized material, uniformly stirring, and carrying out a hydrothermal reaction to obtain mixed slurry, namely activated slurry; and mixing the biomass probiotic liquid and the activated slurry, uniformly stirring, and adding compound bacteria for fermentation to obtain the microbial soil conditioner. The preparation process is simple, the ardealite leachate and the ardealite can be co-treated, full resource utilization of the two industrial wastes is realized, and the microbial soil conditioner is prepared through reasonable proportioning, high-temperature thermal activation, hydrothermal reaction and compound microbial synergistic fermentation. The prepared microbial soil conditioner is simple in use method, and can promote the growth of vegetables and remarkably increase the yield of the vegetables.

Description

Technical Field

[0001] The present invention relates to a method for co - utilizing phosphogypsum and phosphogypsum leachate to prepare a microbial soil conditioner, its product and application, and belongs to the field of microbial soil conditioners. Background Art

[0002] Phosphogypsum mainly consists of calcium sulfate and a small amount of elements such as phosphorus and fluorine, while phosphogypsum leachate is rich in soluble sulfates, phosphates and heavy metal ions. Through the metabolic action of microorganisms, sulfur cycle drive, heavy metal passivation and the formation of organic - inorganic composite structures can be achieved. Phosphogypsum provides a calcium source skeleton, and the organic matter in the leachate is transformed into humic acid by microorganisms, enhancing the soil aggregate structure. Some prior arts have proved that phosphogypsum can be used to prepare soil conditioners through neutralization reactions and calcination modification, and the leachate resource utilization technology can extract effective components. Achieving a closed - loop transformation of "solid waste - waste liquid - functional material" through microbial directional regulation conforms to the theoretical framework of solid waste resource utilization. The synchronous resource utilization of phosphogypsum, a solid waste, and its leachate is not only a major breakthrough in the traditional single - raw - material treatment mode, but also a vivid manifestation of the collaborative treatment strategy of "treating waste with waste" in practice. This innovative measure not only conforms to the development trend of circular economy, but also ingeniously constructs a comprehensive technical system covering the full - component resource utilization of phosphogypsum. This system not only shows high rationality at the scientific - theory level, but also has good operability and implementability in engineering practice, opening up a new path for the resource utilization of phosphogypsum solid waste.

[0003] In terms of raw - material coordination, this technical system regards phosphogypsum solid waste and its leachate as an inseparable whole. Through scientific proportioning and process design, effective coordination of the two in the resource - utilization process is achieved. This coordination not only improves the resource utilization efficiency, but also reduces the energy consumption and emissions in the waste - treatment process, thus promoting green production and sustainable development at the source. From the perspective of social value, this innovative technical system covers three major fields: agriculture, environmental protection and resources. In the agricultural field, the resource - utilized product can be used as a high - quality soil conditioner and fertilizer, improving soil fertility and crop yields; in the environmental - protection field, this technical system effectively reduces industrial waste emissions and pollution, making a positive contribution to environmental protection; in the resource field, through resource utilization, waste is turned into treasure, providing a new way for resource conservation and recycling.

[0004] In addition, the environmental - protection benefits of this technical system are also very significant. It not only reduces the pollution and damage of phosphogypsum solid waste to the environment, but also reduces the dependence on and consumption of primary resources through resource utilization, which not only reflects the important role of technological innovation in environmental protection, but also demonstrates China's firm determination and practical actions in promoting green development and ecological - civilization construction. Summary of the Invention

[0005] Object of the Invention: The technical problem to be solved by the present invention is to provide a method for co-using phosphogypsum and phosphogypsum leachate to prepare a microbial soil conditioner, as well as its product and application.

[0006] Technical Solution: To solve the above technical problem, the present invention provides a method for co-using phosphogypsum and phosphogypsum leachate to prepare a microbial soil conditioner, comprising the following steps:

[0007] (1) Mix phosphogypsum leachate and white mud paste, stir evenly, dry, and activate at high temperature to obtain an activated material; the white mud paste is a mixture of white mud and phosphogypsum;

[0008] (2) Mix sulfide slag, fly ash and the activated material in step (1), stir evenly, granulate, and age to obtain a material to be stabilized;

[0009] (3) Mix papermaking stock preparation waste residue, mushroom residue and the material to be stabilized in step (2), stir evenly, and carry out hydrothermal reaction. After the reaction, the obtained mixed pulp is an activated pulp;

[0010] (4) Mix biomass probiotic liquid and the activated pulp in step (3), stir evenly, add composite bacteria for fermentation to obtain a microbial soil conditioner; the biomass probiotic liquid is a mixed liquid of blueberry juice, rosa roxburghii raw pulp and concentrated apple juice; the composite bacteria are composed of bacillus, streptomyces, vesicular arbuscular mycorrhizal fungi, and aspergillus.

[0011] Among them, the mass ratio of the white mud to the phosphogypsum in step (1) is 20 - 60:100.

[0012] Among them, the liquid-solid ratio of the phosphogypsum leachate to the white mud paste in step (1) is 20 - 60:100 mL / g.

[0013] Among them, the time for high-temperature activation in step (1) is 0.5 - 4.5 hours, and the temperature is 450 - 950 °C.

[0014] Among them, the mass ratio of the sulfide slag, fly ash and the activated material in step (2) is 10 - 30:15 - 45:100.

[0015] Among them, the aging time in step (2) is 6 - 24 hours.

[0016] Among them, the mass ratio of the papermaking stock preparation waste residue, mushroom residue and the material to be stabilized in step (3) is 10 - 30:10 - 40:100.

[0017] Among them, the water-solid ratio of the hydrothermal reaction in step (3) is 2 - 10:1 mL / g, the time is 2 - 6 hours, and the temperature is 120 - 360 °C.

[0018] Among them, the volume ratio of the blueberry juice, the Rosa roxburghii Tratt pulp and the concentrated apple juice described in step (4) is 5-15:5-15:100.

[0019] Among them, the mass ratio of the biological probiotic liquid and the activated pulp described in step (4) is 2.5-7.5:100.

[0020] Among them, the mass of the composite bacteria described in step (4) accounts for 0.025%-0.575% of the total mass of the biological probiotic liquid, the activated pulp and the composite bacteria.

[0021] Among them, the composite bacteria described in step (4) are all bacterial powders.

[0022] Among them, the mass ratio of the Bacillus, Streptomyces, Saccharomycopsis, and Aspergillus described in step (4) is 0.5-7.5:0.5-5.5:0.25-1.75:1.

[0023] Among them, the Bacillus described in step (4) is any one of Bacillus firmus, Bacillus megaterium, Bacillus niacini, Bacillus microflavus, Geobacillus stearothermophilus, Halobacillus halophilus, Bacillus stamsii, Halobacillus salifodinae, Aerobacillus, Borophilus, Bacillus subtilis, Paenibacillus borealis, Bacillus thuringiensis, Bacillus aryabhattai, Bacillus amyloliquefaciens, Bacillus sonorensis, Bacillus paralicheniformis, Bacillus nealsonii, Bacillus aryabhattai or Bacillus thiaminolyticus.

[0024] Among them, the Streptomyces described in step (4) is any one of Streptomyces avermitilis, Streptomyces pactum, Streptomyces flavidofuscus, Streptomyces nigrosporeus, Streptomyces thermoalkalophilus, Streptomyces albidoflavus, Streptomyces fradiae, Streptomyces vinaceusdrappus, Streptomyces herbarum, Streptomyces thermophilus, Streptomyces fengii, Streptomyces demaniae, Streptomyces hiroshimensis, Streptomyces indicus, Streptomyces luteogriseus, Streptomyces cinnamoneus.

[0025] Among them, the Saccharomycopsis described in step (4) is any one of Saccharomycopsis abnormis var. caucasica, Saccharomycopsis nigra, Ashbya gossypii, Saccharomycopsis altissima, Saccharomycopsis abnormis caucasica or Ashbya polyspora; the Aspergillus described in step (4) is any one of Aspergillus niger, Aspergillus sydowii, Aspergillus terreus, Aspergillus wentii, Aspergillus japonicus, Monascus ruber, Aspergillus usamii, Aspergillus oryzae, Aspergillus amstelodami, Aspergillus ficuum, Aspergillus carbonarius, Aspergillus nidulans or Monascus purpureus.

[0026] The temperature of the fermentation described in step (4) is 15-55°C, and the time is 5-25 days.

[0027] The present invention also provides a microbial soil conditioner prepared by the above method.

[0028] The present invention also provides the application of the microbial soil conditioner in increasing plant yields.

[0029] Reaction mechanism:

[0030] When a mixture of white mud, phosphogypsum, and phosphogypsum leachate undergoes high-temperature activation in a high-temperature environment, a series of complex chemical reactions occur. The main component of white mud is calcium hydroxide, which undergoes a dehydration reaction at high temperatures to form calcium oxide, having a relatively high reactivity and can further participate in other reactions. The main component of phosphogypsum is calcium sulfate dihydrate, which undergoes a dehydration reaction at high temperatures to form anhydrous calcium sulfate. Phosphogypsum leachate mainly contains phosphates, fluorides, sulfate radicals, etc. At high temperatures, phosphates can react with calcium oxide to form hydroxyapatite, and fluorides can react with calcium oxide to form calcium fluoride. The combined action of these reactions results in an activated material with relatively high reactivity and stability. Mixing sulfide slag, fly ash, and the activated material, the fine particles in the fly ash can be mixed with other components such as sulfide slag to form a more uniform particle distribution. Ions in the material will undergo exchange, making the chemical composition of the material more uniform and significantly improving the stability and homogeneity of the material. At the same time, during the aging process, calcium oxide will react with water to form calcium hydroxide and release heat, thereby increasing the alkalinity of the material. A large amount of silicates and aluminates in fly ash can react with calcium hydroxide in water to form calcium silicate hydrate and calcium aluminate hydrate gels. Calcium fluoride can further react with free phosphates during the aging process to form fluoridated hydroxyapatite, which is encapsulated in the calcium silicate hydrate and calcium aluminate hydrate gels. Mixing papermaking stock preparation waste residue, mushroom residue, and the material to be stabilized, stirring evenly, and adding them to a reaction kettle for hydrothermal reaction. Some of the organic substances in the mushroom residue will undergo a carbonization reaction under hydrothermal conditions to form carbonaceous hydrochar, and some organic substances will decompose to form small-molecule organic acids such as acetic acid, propionic acid, and butyric acid. Lignin and cellulose in the mushroom residue will decompose under hydrothermal conditions to form phenolic compounds, and the protein-rich part will be partially hydrolyzed to form amino acids and small-molecule peptides and will further be transformed into humus-like substances such as fulvic acid and humic acid. The mushroom residue also contains various sugars, polyphenolic substances, and bioactive substances, and these substances will be partially decomposed and transformed into other organic substances under hydrothermal conditions. Some of the organic substances contained in the papermaking stock preparation waste residue will undergo carbonization to form a carbon-based surface under the hydrothermal environment, and some organic substances will thermally decompose to release small-molecule organic acids, phenolic compounds, ketones, aldehydes, etc. The alkaline substances, calcium-based minerals, and aluminosilicates in the stabilized material will react under hydrothermal conditions to form hydrated products such as calcium silicate hydrate and tobermorite, and these products do not adsorb organic products (amino acids, small-molecule peptides, organic acids, polysaccharides, polyphenols, etc.) and encapsulate the formed carbon-based substances.Mix blueberry juice, rosa roxburghii tratt. original pulp, and concentrated apple juice. During the stirring process, various vitamins (vitamin C, vitamin K, vitamin A, vitamin E, thiamine, riboflavin, thiamine, niacin, B12, and pantothenic acid), minerals (manganese, copper, potassium), and proteins contained in the blueberry juice, fibers, sucrose, glucose, fructose, various natural antioxidants, vitamins (vitamin C, β-carotene) contained in the rosa roxburghii tratt. original pulp, and proteins, dietary fiber, folic acid, minerals (sodium, magnesium, phosphorus, potassium, calcium, iron, zinc, iodine), vitamin A1, vitamin B2, niacin (nicotinamide), vitamin C, fructose, glucose, sucrose, and sorbitol contained in the concentrated apple juice are fully mixed and react to generate derivative active products. Mix the biological mass probiotic liquid and the activated pulp, and add compound bacteria for fermentation. The compound microorganisms attach to the carbon-based substances, hydrated products, and the organic matter of undecomposed lignin, cellulose, and papermaking stock preparation waste residue. During the fermentation process, Bacillus, Streptomyces, arbuscular mycorrhizal fungi, and Aspergillus play their respective roles, jointly promoting the decomposition of the substrate and the generation of metabolites and promoting the reaction between the metabolites and the original active substances and metabolites through enzymatic reactions. During the fermentation period, the four microorganisms first cooperate to utilize various sugars, vitamins, minerals, and active substances in the mixed pulp for reproduction and stimulate the secretion of various extracellular enzymes (amylase, protease, cellulase, hemicellulase, ligninase, phosphatase). The synergistic action of different enzymes and the synergistic action between different enzyme-producing bacteria promote the transformation of refractory substances in the original mushroom residue and papermaking stock preparation waste residue and the generation of new complex active substances. At the same time, during the fermentation process, Bacillus can also utilize various active substances in the biological mass probiotic liquid to produce various antibacterial substances and active enzymes, thereby promoting the reproduction and metabolic activities of Streptomyces, arbuscular mycorrhizal fungi, and Aspergillus and inhibiting the competitive reproduction of other harmful bacteria. Streptomyces can utilize various active substances in the biological mass probiotic liquid and the metabolic substances of other microorganisms to generate antibiotics, various bioactive substances, and plant growth hormones, which is beneficial to improving the performance of the microbial fermentation soil. Arbuscular mycorrhizal fungi can produce auxin, cytokinin, etc., and decompose phosphates and proteins in phosphogypsum to generate soluble phosphorus and amino acids, thereby strengthening the metabolic functions of other microorganisms. Aspergillus can produce various organic acids, alcohols, ketones and other metabolites to promote the growth of other microorganisms.

[0031] Beneficial effects: Compared with the prior art, the present invention has the following remarkable advantages: The preparation process of the present invention is simple, can co-dispose phosphogypsum leachate and phosphogypsum, and realizes the full resource utilization of the two industrial wastes. By reasonable batching, high-temperature thermal activation, hydrothermal reaction, and compound microorganism co-fermentation, a microbial soil conditioner is prepared. The prepared microbial soil conditioner has a simple usage method, can promote the growth of vegetables, and significantly improve the vegetable yield. Description of the Drawings

[0032] Figure 1This is the flow chart of the present invention. Detailed implementation manners

[0033] The technical solution of the present invention will be further described below with reference to the accompanying drawings.

[0034] White mud: The white mud is taken from Guizhou Chitianhua Paper Co., Ltd. The main components in the white mud sample are 81.26% CaCO3, 9.94% NaOH, 4.15% SiO2, 2.78% Al2O3 and 1.87% fiber residue.

[0035] Sulphide slag: The sulphide slag is taken from Guixi Smelter of Jiangxi Copper Corporation. The main components in the sulphide slag sample are 57.14% Fe2O3, 31.67% SiO2, 10.39% Al2O3, 0.45% SnS2 and other components (inevitable impurities and loss on ignition).

[0036] Fly ash: It comes from Taicang Power Plant of Huaneng International Power Co., Ltd., mainly including 43.21% SiO2, 27.08% Al2O3, 15.62% Fe2O3, 6.58% CaO, 3.42% TiO2, 1.43% SO3, 1.04% K2O, 0.63% Na2O and other components (inevitable impurities and loss on ignition).

[0037] Paper-making stock preparation waste residue: The paper-making stock preparation waste residue is taken from Guizhou Chitianhua Paper Co., Ltd. The main components in the paper-making stock preparation waste residue are 74.36% cellulose / lignin, 17.29% SiO2, 2.1% Na2S and 6.25% resin / pectin.

[0038] Mushroom residue: The mushroom residue comes from Guizhou Guijun Agricultural Science and Technology Development Co., Ltd. The mushroom residue contains 72.46% organic matter, 2.34% total nitrogen, 1.63% total phosphorus, 2.17% total potassium and others (moisture and trace minerals).

[0039] Phosphogypsum: The phosphogypsum is taken from Guizhou Xifeng Phosphate Mine Co., Ltd. The main components in the phosphogypsum sample are 52.70% SO3, 37.01% CaO, 4.37% SiO2, 2.07% Al2O3, 1.63% P2O5 and other components (inevitable impurities and loss on ignition).

[0040] Phosphogypsum leachate: The phosphogypsum stack leachate is obtained by sampling from the on-site collection pool in the phosphogypsum stack yard in Xifeng County, Guizhou. The pH is 2.23, the total phosphorus is 816 mg / L, the sulfate ion concentration is 2146 mg / L, the calcium ion concentration is 439.51 mg / L, the magnesium ion concentration is 564.89 mg / g, and the fluoride concentration is 91.37 mg / g.

[0041] Example 1 Influence of the mass ratio of sulfide slag, fly ash, and activated material on the performance of the prepared microbial soil conditioner

[0042] Mix white mud and phosphogypsum in a mass ratio of 20:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 20:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain activated material, where the activation time is 0.5 hours and the activation temperature is 450 °C. Mix sulfide slag, fly ash, and activated material in mass ratios of 2.5:15:100, 5:15:100, 7.5:15:100, 10:7.5:100, 10:10:100, 10:12.5:100, 10:15:100, 20:15:100, 30:15:100, 10:30:100, 20:30:100, 30:30:100, 10:45:100, 20:45:100, 30:45:100, 30:50:100, 30:55:100, 30:60:100, 32.5:45:100, 35:45:100, 37.5:45:100, stir evenly, granulate, and age for 6 hours to obtain the material to be stabilized. Mix papermaking stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 10:10:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the resulting mixed slurry after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 2:1 mL / g, the hydrothermal time is 2 hours, and the hydrothermal temperature is 120 °C. Mix blueberry juice (raw pulp), Rosa roxburghii Tratt raw pulp, and concentrated apple juice (65 Brix) in a volume ratio of 5:5:100, stir evenly to obtain the biomass probiotic liquid. Mix the biomass probiotic liquid and the activated slurry in a mass ratio of 2.5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain the microbial soil conditioner, where the fermentation temperature is 15 °C, the fermentation time is 5 days, the composite bacteria are composed of Bacillus, Streptomyces, Sclerocystis, and Aspergillus, where the mass ratio of the added composite microorganisms is 0.025%, and the mass ratio of Bacillus, Streptomyces, Sclerocystis, and Aspergillus is 0.5:0.5:0.25:1. The Bacillus is Bacillus firmus (CGMCC 1.16095); the Streptomyces is Streptomyces avermitilis (CGMCC 4.7296); the Sclerocystis is Sclerocystis abnormis var. caucasica (CGMCC 3.6636); the Aspergillus is Aspergillus niger (CGMCC 3.15663).

[0043] Comparative Experiment on the Cultivation of Lettuce and Leaf Lettuce: Select two identical plots of land for cultivating lettuce and leaf lettuce, namely Plot No.1 and Plot No.2. The processes of seedling selection, planting, and plant protection for lettuce or leaf lettuce are the same. During the entire growth period, no microbial soil conditioner is applied to Plot No.1, while microbial soil conditioner (2 kg per square meter) is applied to Plot No.2 before transplanting seedlings. After the planting period ends, harvest the lettuce or leaf lettuce, wash, dry, and weigh them.

[0044] Lettuce Yield Increase Rate: The weight difference between the lettuce harvested from Plot No.2 and the lettuce harvested from Plot No.1 is divided by the weight of the lettuce harvested from Plot No.1 to obtain the lettuce yield increase rate.

[0045] Leaf Lettuce Yield Increase Rate: The weight difference between the leaf lettuce harvested from Plot No.2 and the leaf lettuce harvested from Plot No.1 is divided by the weight of the leaf lettuce harvested from Plot No.1 to obtain the leaf lettuce yield increase rate.

[0046] The experimental results of this example are shown in Table 1.

[0047] Table 1 Influence of the Mass Ratio of Sulfide Residue, Fly Ash, and Activated Material on the Performance of the Prepared Microbial Soil Conditioner

[0048]

[0049] As can be seen from Table 1, when the mass ratio of sulfide slag, fly ash, and activator is less than 10:15:100 (as shown in Table 1, the mass ratio of sulfide slag, fly ash, and activator = 7.5:15:100, 5:15:100, 2.5:15:100, 10:12.5:100, 10:10:100, 10:7.5:100 and lower ratios not listed in Table 1), the addition of sulfide slag and fly ash is less, and the material reaction is unbalanced during the later hydrothermal process, resulting in a decrease in the performance of the prepared microbial soil conditioner. The yield increase rates of lettuce and romaine lettuce obtained from planting both decrease significantly as the mass ratio of sulfide slag, fly ash, and activator decreases. When the mass ratio of sulfide slag, fly ash, and activator is equal to 10 - 30:15 - 45:100 (as shown in Table 1, the mass ratio of sulfide slag, fly ash, and activator = 10:15:100, 20:15:100, 30:15:100, 10:30:100, 20:30:100, 30:30:100, 10:45:100, 20:45:100, 30:45:100), a series of complex chemical reactions will occur when the mixture of white mud, phosphogypsum, and phosphogypsum leachate is subjected to high-temperature activation in a high-temperature environment. The main component of white mud is calcium hydroxide, which will undergo a dehydration reaction at high temperature to form calcium oxide, having a high reaction activity and can further participate in other reactions. The main component of phosphogypsum is calcium sulfate dihydrate, which will undergo a dehydration reaction at high temperature to form anhydrous calcium sulfate. The phosphogypsum leachate mainly contains phosphates, fluorides, sulfate radicals, etc. At high temperature, phosphates can react with calcium oxide to form hydroxyapatite, and fluorides can react with calcium oxide to form calcium fluoride. Through the combined action of the above reactions, the generated activator has high reaction activity and stability. When sulfide slag, fly ash, and activator are mixed, the fine particles in fly ash can be mixed with other components such as sulfide slag to form a more uniform particle distribution. Ions in the material will undergo exchange, making the chemical composition of the material more uniform, and significantly improving the stability and homogeneity of the material. At the same time, during the aging process, calcium oxide will react with water to form calcium hydroxide and release heat, thereby increasing the alkalinity of the material. A large amount of silicates and aluminates in fly ash can react with calcium hydroxide in water to form calcium silicate hydrate and calcium aluminate hydrate gels. Calcium fluoride can further react with free phosphates during the aging process to form fluorohydroxyapatite, which is encapsulated in the calcium silicate hydrate and calcium aluminate hydrate gels. Finally, the yield increase rate of lettuce is higher than 123% and the yield increase rate of romaine lettuce is higher than 134%.When the mass ratio of sulfide slag, fly ash, and activator is greater than 30:45:100 (as shown in Table 1, when the mass ratio of sulfide slag, fly ash, and activator = 30:50:100, 30:55:100, 30:60:100, 32.5:45:100, 35:45:100, 37.5:45:100 and higher ratios not listed in Table 1), the addition of sulfide slag and fly ash is excessive, and the material reaction is unbalanced during the hydrothermal reaction, resulting in a decrease in the performance of the prepared microbial soil conditioner. The yield increase rates of lettuce and lettuce obtained by planting both decrease significantly as the mass ratio of sulfide slag, fly ash, and activator further increases.

[0050] Therefore, generally speaking, considering both benefits and costs, when the mass ratio of sulfide slag, fly ash, and activator is equal to 10 - 30:15 - 45:100, it is most beneficial to improve the performance of the prepared microbial soil conditioner.

[0051] Example 2 Influence of the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized on the performance of the prepared microbial soil conditioner

[0052] Mix white mud and phosphogypsum in a mass ratio of 40:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 40:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain activated material, where the activation time is 2.5 hours and the activation temperature is 750 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 15 hours to obtain the material to be stabilized. Mix papermaking stock preparation waste residue, mushroom residue, and the material to be stabilized in mass ratios of 2.5:10:100, 5:10:100, 7.5:10:100, 10:2.5:100, 10:5:100, 10:7.5:100, 10:10:100, 20:10:100, 30:10:100, 10:25:100, 20:25:100, 30:25:100, 10:40:100, 20:40:100, 30:40:100, 30:45:100, 30:50:100, 30:55:100, 32.5:40:100, 35:40:100, 37.5:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the resulting mixed slurry after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 6:1 mL / g, the hydrothermal time is 4 hours, and the hydrothermal temperature is 240 °C. Mix blueberry juice (raw pulp), rosa roxburghii tratt raw pulp, and concentrated apple juice (67.5 Brix) in a volume ratio of 10:10:100, stir evenly to obtain the biomass probiotic liquid. Mix the biomass probiotic liquid and the activated slurry in a mass ratio of 5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain the microbial soil conditioner, where the fermentation temperature is 35 °C, the fermentation time is 15 days, the composite bacteria are composed of bacillus, streptomyces, endogone, and aspergillus, where the mass ratio of the added composite microorganisms is 0.3%, and the mass ratio of bacillus, streptomyces, endogone, and aspergillus is 4:3:1:1. The bacillus is bacillus megaterium (CGMCC 1.16094); the streptomyces is streptomyces pactum (CGMCC 4.6287); the endogone is endogone nigricans (CGMCC 3.6637); the aspergillus is aspergillus sydowii (CGMCC 3.13944).

[0053] The comparative experiment on the cultivation of lettuce and lettuce, and the calculation of the yield increase rate of lettuce and the yield increase rate of lettuce are the same as in Example 1. The test results of this example are shown in Table 2.

[0054] Table 2 Influence of the mass ratio of papermaking stock preparation waste residue, mushroom residue, and the material to be stabilized on the performance of the prepared microbial soil conditioner

[0055]

[0056]

[0057] As can be seen from Table 2, when the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized is less than 10:10:100 (as in Table 2, the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized = 7.5:10:100, 5:10:100, 2.5:10:100, 10:7.5:100, 10:5:100, 10:2.5:100 and lower ratios not listed in Table 2), the addition of papermaking stock preparation waste residue and mushroom residue is less, and the material reaction is unbalanced during the subsequent hydrothermal and fermentation processes, resulting in a decrease in the performance of the prepared microbial soil conditioner. The yield increase rates of lettuce and romaine lettuce obtained from planting both significantly decrease as the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized decreases. When the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized is equal to 10 - 30:15 - 45:100 (as in Table 2, the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized = 10:15:100, 20:15:100, 30:15:100, 10:30:100, 20:30:100, 30:30:100, 10:45:100, 20:45:100, 30:45:100), the papermaking stock preparation waste residue, mushroom residue, and material to be stabilized are mixed and stirred evenly, and then added to a reaction kettle for hydrothermal reaction. Some of the organic substances in the mushroom residue will undergo a carbonization reaction under hydrothermal conditions to form carbonaceous hydrochar, and some organic substances will decompose to form small molecule organic acids such as acetic acid, propionic acid, and butyric acid. The lignin and cellulose in the mushroom residue will decompose to form phenolic compounds under hydrothermal conditions, and the protein it is rich in will be partially hydrolyzed to form amino acids and small molecule peptides and will further be converted into humus-like substances such as fulvic acid and humic acid. The mushroom residue also contains various sugars, polyphenols, and bioactive substances, and these substances will be partially decomposed and converted into other organic substances under hydrothermal conditions. Some of the organic substances contained in the papermaking stock preparation waste residue will undergo carbonization to form a carbon-based surface under the hydrothermal environment, and some organic substances will thermally decompose to release small molecule organic acids, phenolic compounds, ketones, aldehydes, etc. The alkaline substances, calcium-based minerals, and aluminosilicates in the stabilized material will react to form hydrated products such as calcium silicate hydrate and tobermorite under hydrothermal conditions, and these products do not adsorb organic products (amino acids, small molecule peptides, organic acids, polysaccharides, polyphenols, etc.) and wrap the formed carbon-based substances. Finally, the yield increase rate of lettuce is higher than 149% and the yield increase rate of romaine lettuce is higher than 175%.When the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized is greater than 30:40:100 (as shown in Table 2, when the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized = 30:45:100, 30:50:100, 30:55:100, 32.5:40:100, 35:40:100, 37.5:40:100 and higher ratios not listed in Table 2), the addition of papermaking stock preparation waste residue and mushroom residue is excessive, and the material reaction is unbalanced during the hydrothermal reaction, resulting in a decline in the performance of the prepared microbial soil conditioner. The yield increase rates of the lettuce and lettuce obtained by planting both decrease significantly as the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized further increases.

[0058] Therefore, generally speaking, considering the benefits and costs, when the mass ratio of papermaking stock preparation waste residue, mushroom residue, and material to be stabilized is equal to 10 - 30:10 - 40:100, it is most beneficial to improve the performance of the prepared microbial soil conditioner.

[0059] Effect of the volume ratio of blueberry juice, Rosa roxburghii puree, and concentrated apple juice on the performance of the prepared microbial soil conditioner in Example 3

[0060] Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain an activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain a material to be stabilized. Mix papermaking stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the resulting mixed slurry after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), Rosa roxburghii Tratt raw pulp, and concentrated apple juice (70 Brix) in volume ratios of 3.5:5:100, 4:5:100, 4.5:5:100, 5:3.5:100, 5:4:100, 5:4.5:100, 5:5:100, 10:5:100, 15:5:100, 5:10:100, 10:10:100, 15:10:100, 5:15:100, 10:15:100, 15:15:100, 15:17.5:100, 15:20:100, 15:22.5:100, 17.5:15:100, 20:15:100, 22.5:15:100, stir evenly to obtain a biological probiotic liquid. Mix the biological probiotic liquid and the activated slurry in a mass ratio of 7.5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain a microbial soil conditioner, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacteria are composed of Bacillus, Streptomyces, Endogone, and Aspergillus, where the mass ratio of the added composite microorganisms is 0.575%, and the mass ratio of Bacillus, Streptomyces, Endogone, and Aspergillus is 7.5:5.5:1.75:1. The Bacillus is Bacillus nealsonii (CGMCC 1.16092); the Streptomyces is Streptomyces flavogriseus (CGMCC 4.6391); the Endogone is Endogone flocculosa (CGMCC 2.2920); the Aspergillus is Aspergillus terreus (CGMCC 3.15736).

[0061] The comparative experiment on the cultivation of lettuce and lettuce, and the calculation of the yield increase rate of lettuce and the yield increase rate of lettuce are the same as in Example 1. The test results of this example are shown in Table 3.

[0062] Table 3 Influence of the volume ratio of blueberry juice, Rosa roxburghii Tratt raw pulp, and concentrated apple juice on the performance of the prepared microbial soil conditioner

[0063]

[0064]

[0065] As can be seen from Table 3, when the volume ratio of blueberry juice, Rosa roxburghii Tratt pulp, and concentrated apple juice is less than 5:5:100 (as in Table 3, the volume ratio of blueberry juice, Rosa roxburghii Tratt pulp, and concentrated apple juice = 4.5:5:100, 4:5:100, 3.5:5:100, 5:4.5:100, 5:4:100, 5:3.5:100, and lower ratios not listed in Table 3), the addition of blueberry juice and Rosa roxburghii Tratt pulp is less, and the material reaction is unbalanced during the later fermentation process, resulting in a decrease in the performance of the prepared microbial soil conditioner. The yield increase rates of lettuce and endive obtained from planting both significantly decrease as the volume ratio of blueberry juice, Rosa roxburghii Tratt pulp, and concentrated apple juice decreases. When the volume ratio of blueberry juice, Rosa roxburghii Tratt pulp, and concentrated apple juice is equal to 5 - 15:5 - 15:100 (as in Table 3, the volume ratio of blueberry juice, Rosa roxburghii Tratt pulp, and concentrated apple juice = 5:5:100, 10:5:100, 15:5:100, 5:10:100, 10:10:100, 15:10:100, 5:15:100, 10:15:100, 15:15:100), the mixed blueberry juice, Rosa roxburghii Tratt pulp, and concentrated apple juice are fully mixed during the stirring process. The various vitamins (vitamin C, vitamin K, vitamin A, vitamin E, thiamine, riboflavin, thiamine, niacin, B12, and pantothenic acid), minerals (manganese, copper, potassium), and proteins contained in the blueberry juice, the fiber, sucrose, glucose, fructose, various natural antioxidants, vitamins (vitamin C, β-carotene) contained in the Rosa roxburghii Tratt pulp, and the proteins, dietary fiber, folic acid, minerals (sodium, magnesium, phosphorus, potassium, calcium, iron, zinc, iodine), vitamin A1, vitamin B2, niacin (nicotinamide), vitamin C, fructose, glucose, sucrose, and sorbitol contained in the concentrated apple juice react to form derivative active products. The mixed biomass probiotic liquid and activated pulp are added with composite bacteria for fermentation. The composite microorganisms attach to the carbon-based substances, hydrated products, and the organic matter of undecomposed lignin, cellulose, and papermaking stock preparation waste residue. During the fermentation process, Bacillus, Streptomyces, Saccobolus, and Aspergillus play their respective roles, jointly promoting the decomposition of the substrate and the generation of metabolites and promoting the reaction between the metabolites and the original active substances and metabolites through enzymatic reactions. During the fermentation period, the four microorganisms first synergistically utilize various sugars, vitamins, minerals, and active substances in the mixed pulp for reproduction and stimulate the secretion of various extracellular enzymes (amylase, protease, cellulase, hemicellulase, ligninase, phosphatase). The synergistic action of different enzymes and the synergistic action between different enzyme-producing bacteria promote the transformation of refractory substances in the original mushroom residue and papermaking stock preparation waste residue and the generation of new complex active substances. At the same time, during the fermentation process, Bacillus can also use various active substances in the biomass probiotic liquid to produce various antibacterial substances and active enzymes, thereby promoting the reproduction and metabolic activities of Streptomyces, Saccobolus, and Aspergillus and inhibiting the competitive reproduction of other harmful bacteria.Streptomyces can utilize various active substances in the biomass probiotic liquid and the metabolic substances of other microorganisms to produce antibiotics, various bioactive substances, and plant growth hormones, thereby facilitating the improvement of the performance of microbial fermented soil. Glomus can produce auxin, cytokinin, etc., and decompose phosphogypsum phosphate and proteins to generate soluble phosphorus and amino acids, thereby enhancing the metabolic functions of other microorganisms. Aspergillus can produce various organic acids, alcohols, ketones and other metabolites to promote the growth of other microorganisms. Finally, the yield increase rate of lettuce is higher than 170% and that of lettuce is higher than 194%. When the volume ratio of blueberry juice, rosa roxburghii pulp, and concentrated apple juice is greater than 15:15:100 (as shown in Table 3, the volume ratio of blueberry juice, rosa roxburghii pulp, and concentrated apple juice = 15:17.5:100, 15:20:100, 15:22.5:100, 17.5:15:100, 20:15:100, 22.5:15:100 and higher ratios not listed in Table 3), the addition of blueberry juice and rosa roxburghii pulp is excessive, and the material reaction is unbalanced during the fermentation process, resulting in a decline in the performance of the prepared microbial soil conditioner. The yield increase rates of lettuce and lettuce obtained by planting both decrease significantly as the volume ratio of blueberry juice, rosa roxburghii pulp, and concentrated apple juice further increases.

[0066] Therefore, generally speaking, considering the benefits and costs, when the volume ratio of blueberry juice, rosa roxburghii pulp, and concentrated apple juice is equal to 5 - 15:5 - 15:100, it is most beneficial to improve the performance of the prepared microbial soil conditioner.

[0067] Example 4 Influence of Bacillus on the Performance of the Prepared Microbial Soil Conditioner

[0068] Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain the material to be stabilized. Mix papermaking stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the mixed slurry obtained after the reaction is activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), roselle raw pulp, and concentrated apple juice (67.5 Brix) in a volume ratio of 15:15:100, stir evenly to obtain a biomass probiotic liquid.Mix the biomass probiotic liquid and the activation pulp in a mass ratio of 7.5:100, stir evenly, add the composite bacteria for fermentation. After the fermentation is completed, a microbial soil conditioner is obtained. The fermentation temperature is 55 °C and the fermentation time is 25 days. The composite bacteria are composed of Bacillus, Streptomyces, Glomus, and Aspergillus. The mass ratio of the added composite microorganisms is 0.3%. The mass ratio of Bacillus, Streptomyces, Glomus, and Aspergillus is 0.5:5.5:1:1. The Bacillus is any one of Bacillus firmus (CGMCC 1.16095), Bacillus megaterium (CGMCC 1.16094), Bacillus niacini (CGMCC 1.16092), Bacillus subflavus (CGMCC 1.16090), Geobacillus stearothermophilus (CGMCC 1.16087), Bacillus halophilus (CGMCC 1.16083), Bacillus stamsii (CGMCC 1.16082), Halobacillus salinus (CGMCC 1.15964), Bacillus aerius (CGMCC 1.15932), Bacillus boroniphilus (CGMCC 1.15846), Bacillus subtilis (CGMCC 1.821), Paenibacillus borealis (CGMCC 1.15729), Bacillus thuringiensis (CGMCC 1.15822), Bacillus aryabhattai (CGMCC 1.15821), Bacillus amyloliquefaciens (CGMCC 1.857), Bacillus sonorensis (CGMCC 1.15888), Bacillus paralicheniformis (CGMCC 1.15832), Bacillus nealsonii (CGMCC 1.16140), Bacillus aryabhattai (CGMCC 1.15821), Bacillus thiaminolyticus (CGMCC 1.16118); the Streptomyces is Streptomyces nigrosporeus (CGMCC 4.7390); the Glomus is Glomus maximus (CGMCC 3.6638); the Aspergillus is Aspergillus wentii (CGMCC 3.15268).

[0069] The planting comparison tests of lettuce and lettuce mustard, and the calculation of the yield increase rates of lettuce and lettuce mustard are the same as those in Example 1. The test results of this example are shown in Table 4.

[0070] Table 4 Influence of Bacillus on the performance of the prepared microbial soil conditioner

[0071]

[0072]

[0073] As can be seen from Table 4, when the Bacillus is any one of Bacillus firmus, Bacillus megaterium, Bacillus niacini, Bacillus microflavus, Geobacillus stearothermophilus, Halobacillus halophilus, Stamsella stamsii, Halobacillus salifodinae, Aerobacillus aerogenes, Bacillus boroniphilus, Bacillus subtilis, Paenibacillus borealis, Bacillus thuringiensis, Bacillus aryabhattai, Bacillus amyloliquefaciens, Bacillus sonorensis, Bacillus paralicheniformis, Bacillus nealsonii, Bacillus aryabhattai, Bacillus aneurinolyticus, there is no significant difference in the yield increase rates of lettuce and romaine lettuce obtained by applying the microbial soil conditioner for planting.

[0074] Example 5 Effect of Streptomyces on the Performance of the Prepared Microbial Soil Conditioner

[0075] Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain an activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain a material to be stabilized. Mix papermaking stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction. After the reaction, the obtained mixed slurry is an activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), Rosa roxburghii Tratt raw pulp, and concentrated apple juice (67.5 Brix) in a volume ratio of 15:15:100, stir evenly to obtain a biomass probiotic liquid. Mix the biomass probiotic liquid and the activated slurry in a mass ratio of 7.5:100, stir evenly, add composite bacteria for fermentation. After the fermentation, a microbial soil conditioner is obtained, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacteria are composed of Bacillus, Streptomyces, Saccobolus, and Aspergillus, the mass ratio of the added composite microorganisms is 0.3%, and the mass ratio of Bacillus, Streptomyces, Saccobolus, and Aspergillus is 7.5:3:0.25:1. The Bacillus is Geobacillus stearothermophilus (CGMCC 1.16087); the Streptomyces is Streptomyces avermitilis (CGMCC 4.7296), Streptomyces pactum (CGMCC 4.6287), Streptomyces flavidovirens (CGMCC 4.6391), Streptomyces atroviridis (CGMCC 4.7390), Streptomyces thermoalkalophilus (CGMCC 4.6961), Streptomyces albidoflavus (CGMCC 4.7292), Streptomyces fradiae (CGMCC 4.7387), Streptomyces vinaceusdrappus (CGMCC 4.7294), Streptomyces herbarum (CGMCC 4.6835), Streptomyces thermophilus (CGMCC 4.3573), Streptomyces fengii (CGMCC 4.3589), Streptomyces dianae (CGMCC 4.3599), Streptomyces hiroshimensis (CGMCC 4.6966), Streptomyces heterocystosus (CGMCC 4.6836), Streptomyces luteocyclus (CGMCC 4.6973), Streptomyces cinnamoneus (CGMCC 4.6972) any one of them; the Saccobolus is Saccobolus abnormis (CGMCC 3.6656); the Aspergillus is Aspergillus japonicus (CGMCC 3.11442).

[0076] The planting comparison test of lettuce and lettuce, and the calculation of the lettuce yield increase rate and the lettuce yield increase rate are the same as in Example 1. The test results of this example are shown in Table 5.

[0077] Table 5 Influence of Streptomyces on the performance of the prepared microbial soil conditioner

[0078]

[0079]

[0080] As can be seen from Table 5, when the Streptomyces is any one of Streptomyces avermitilis, Streptomyces pactum, Streptomyces flavidofuscus, Nigrospora oryzae, Thermoalkaliphilic Streptomyces, Streptomyces albidoflavus, Streptomyces fradiae, Streptomyces vinaceusdrappus, Streptomyces herbarum, Streptomyces thermophilus, Streptomyces fengii, Streptomyces demainii, Streptomyces hiroshimensis, Streptomyces heterocystus, Streptomyces luteocyclus, Streptomyces cinnamoneus, there is no significant difference in the yield increase rates of lettuce and romaine lettuce obtained by applying the microbial soil conditioner for planting.

[0081] Example 6 Influence of Glomus on the performance of the prepared microbial soil conditioner

[0082] Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain an activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain a material to be stabilized. Mix papermaking stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the mixed slurry obtained after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), Rosa roxburghii Tratt raw pulp, and concentrated apple juice (67.5 Brix) in a volume ratio of 15:15:100, stir evenly to obtain a biomass probiotic liquid. Mix the biomass probiotic liquid and the activated slurry in a mass ratio of 7.5:100, stir evenly, add a composite bacterium for fermentation, and after the fermentation is completed, obtain a microbial soil conditioner, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacterium is composed of Bacillus, Streptomyces, Sclerocystis, and Aspergillus, and the mass ratio of the added composite microorganism is 0.3%, and the mass ratio of Bacillus, Streptomyces, Sclerocystis, and Aspergillus is 4:5.5:1.75:1. The Bacillus is Bacillus halophilus (CGMCC 1.16083); the Streptomyces is Streptomyces albidoflavus (CGMCC 4.7292); the Sclerocystis is any one of Sclerocystis abnormis var. caucasica (CGMCC 3.6636), Sclerocystis atra (CGMCC 3.6637), Ashbya gossypii (CGMCC 2.2920), Sclerocystis altissima (CGMCC 3.6638), Sclerocystis abnormis caucasica (CGMCC 3.6656), and Ashbya polyspora (CGMCC 2.482); the Aspergillus is Monascus ruber (CGMCC 3.7882).

[0083] The comparative test of lettuce and lettuce cultivation and the calculation of the yield increase rate of lettuce and the yield increase rate of lettuce are the same as in Example 1. The test results of this example are shown in Table 6.

[0084] Table 6 Influence of Sclerocystis on the performance of the prepared microbial soil conditioner

[0085] Ascochyta Yield increase rate of lettuce Yield increase rate of lettuce lettuce Ascochyta abnormis var. caucasica 206.78％ 224.67％ Ascochyta atra 204.15％ 225.31％ Ashbya gossypii 200.52％ 222.19％ Ascochyta hypericin 203.46％ 220.48％ Ascochyta abnormis caucasica 205.69％ 225.26％ Ashbya polyspora 203.59％ 223.71％

[0086] As can be seen from Table 6, when the Sclerocystis is any one of Sclerocystis abnormis var. caucasica, Sclerocystis atra, Ashbya gossypii, Sclerocystis altissima, Sclerocystis abnormis caucasica, Gigaspora gigantea, Ashbya polyspora, and Glomus mosseae, there is no significant difference in the yield increase rates of lettuce and lettuce obtained by applying the microbial soil conditioner for cultivation.

[0087] Example 7 Influence of Aspergillus on the Performance of the Prepared Microbial Soil Conditioner

[0088] Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain an activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain a material to be stabilized. Mix paper-making stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the mixed slurry obtained after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), Rosa roxburghii Tratt raw pulp, and concentrated apple juice (67.5 Brix) in a volume ratio of 15:15:100, stir evenly to obtain a biomass probiotic solution. Mix the biomass probiotic solution and the activated slurry in a mass ratio of 7.5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain a microbial soil conditioner, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacteria are composed of Bacillus, Streptomyces, Endogone, and Aspergillus, where the mass ratio of the added composite microorganisms is 0.3%, and the mass ratio of Bacillus, Streptomyces, Endogone, and Aspergillus is 4:3:0.25:1. The Bacillus is Bacillus stamsii (CGMCC 1.16082); the Streptomyces is Streptomyces fradiae (CGMCC 4.7387); the Endogone is Endogone aspergillum (CGMCC 2.482); the Aspergillus is any one of Aspergillus niger (CGMCC 3.15663), Aspergillus sydowii (CGMCC 3.13944), Aspergillus terreus (CGMCC 3.15736), Aspergillus wentii (CGMCC 3.15268), Aspergillus japonicus (CGMCC 3.11442), Monascus ruber (CGMCC 3.7882), Aspergillus usamii (CGMCC 3.7010), Aspergillus oryzae (CGMCC 3.13905), Aspergillus amstelodami (CGMCC 3.17655), Aspergillus ficuum (CGMCC 3.7158), Aspergillus carbonarius (CGMCC 3.7132), Aspergillus nidulans (CGMCC 3.15737), Monascus purpureus (CGMCC 3.15548).

[0089] The comparative test of lettuce and lettuce planting and the calculation of the lettuce yield increase rate and the lettuce yield increase rate are the same as in Example 1. The test results of this example are shown in Table 7.

[0090] Table 7 Influence of Aspergillus on the Performance of the Prepared Microbial Soil Conditioner

[0091] Aspergillus Yield increase rate of lettuce Yield increase rate of lettuce lettuce Aspergillus niger 206.54％ 227.63％ Aspergillus sydowii 205.85％ 223.46％ Aspergillus terreus 202.37％ 221.95％ Aspergillus wentii 204.76％ 224.64％ Aspergillus japonicus 201.96％ 220.82％ Monascus purpureus Went var. ruber (Sato) Hata & Takimoto 199.59％ 224.19％ Aspergillus usamii 205.43％ 221.76％ Aspergillus oryzae 198.81％ 222.95％ Aspergillus amstelodami 202.94％ 220.49％ Aspergillus ficuum 204.21％ 225.51％ Aspergillus carbonarius 203.68％ 221.94％ Aspergillus nidulans 206.39％ 219.72％ Monascus purpureus 204.15％ 218.56％

[0092] As can be seen from Table 7, there is no significant difference in the yield increase rates of lettuce and romaine lettuce obtained by planting with any one of Aspergillus niger, Aspergillus sydowii, Aspergillus terreus, Aspergillus wentii, Aspergillus japonicus, Monascus ruber, Aspergillus usamii, Aspergillus oryzae, Aspergillus amstelodami, Aspergillus ficuum, Aspergillus carbonarius, Aspergillus nidulans, and Monascus purpureus after applying the microbial soil conditioner.

[0093] Influence of different processes in the comparative examples on the performance of the prepared microbial soil conditioner

[0094] The process of the present invention: Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain an activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain a material to be stabilized. Mix papermaking stock waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the mixed slurry obtained after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), Rosa roxburghii raw pulp, and concentrated apple juice (67.5 Brix) in a volume ratio of 15:15:100, stir evenly to obtain a biomass probiotic solution. Mix the biomass probiotic solution and the activated slurry in a mass ratio of 7.5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain a microbial soil conditioner, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacteria are composed of Bacillus, Streptomyces, Endogone, and Aspergillus, the mass ratio of the added composite microorganisms is 0.3%, and the mass ratio of Bacillus, Streptomyces, Endogone, and Aspergillus is 4:3:0.25:1. The Bacillus is Bacillus stamsii (CGMCC 1.16082); the Streptomyces is Streptomyces fradiae (CGMCC 4.7387); the Endogone is Endogone aspergillum (CGMCC 2.482); the Aspergillus is Monascus purpureus (CGMCC 3.15548).

[0095] Comparative Process 1: Mix phosphogypsum leachate and phosphogypsum according to a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain an activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material according to a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain a material to be stabilized. Mix paper-making stock waste residue, mushroom residue, and the material to be stabilized according to a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the resulting mixed slurry after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix blueberry juice (raw pulp), rosa roxburghii tratt raw pulp, and concentrated apple juice (67.5 Brix) according to a volume ratio of 15:15:100, stir evenly to obtain a biomass probiotic liquid. Mix the biomass probiotic liquid and the activated slurry according to a mass ratio of 7.5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain a microbial soil conditioner, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacteria are composed of bacillus, streptomyces, endogone, and aspergillus, where the mass ratio of the added composite microorganisms is 0.3%, and the mass ratio of bacillus, streptomyces, endogone, and aspergillus is 4:3:0.25:1. The bacillus is bacillus stamsii (CGMCC 1.16082); the streptomyces is streptomyces fradiae (CGMCC 4.7387); the endogone is endogone ashbyii (CGMCC 2.482); the aspergillus is monascus purpureus (CGMCC 3.15548).

[0096] Comparative Process 2: Mix white mud and phosphogypsum in a mass ratio of 60:100, stir evenly to obtain white mud paste. Mix phosphogypsum leachate and white mud paste in a liquid-solid ratio of 60:100 mL / g, stir evenly, dry, and place in a furnace for high-temperature activation to obtain activated material, where the activation time is 4.5 hours and the activation temperature is 950 °C. Mix sulfide slag, fly ash, and activated material in a mass ratio of 30:45:100, stir evenly, granulate, and age for 24 hours to obtain the material to be stabilized. Mix papermaking stock preparation waste residue, mushroom residue, and the material to be stabilized in a mass ratio of 30:40:100, stir evenly, add to a reaction kettle for hydrothermal reaction, and the resulting mixed slurry after the reaction is the activated slurry, where the water-solid ratio of the hydrothermal reaction is 10:1 mL / g, the hydrothermal time is 6 hours, and the hydrothermal temperature is 360 °C. Mix water and the activated slurry in a mass ratio of 7.5:100, stir evenly, add composite bacteria for fermentation, and after the fermentation is completed, obtain a microbial soil conditioner, where the fermentation temperature is 55 °C, the fermentation time is 25 days, the composite bacteria are composed of Bacillus, Streptomyces, Saccharomycopsis, and Aspergillus, the mass ratio of the added composite microorganisms is 0.3%, and the mass ratio of Bacillus, Streptomyces, Saccharomycopsis, and Aspergillus is 4:3:0.25:1. The Bacillus is Bacillus stamsii (CGMCC 1.16082); the Streptomyces is Streptomyces fradiae (CGMCC 4.7387); the Saccharomycopsis is Saccharomycopsis asbyii (CGMCC 2.482); the Aspergillus is Monascus purpureus (CGMCC 3.15548).

[0097] The comparative test of lettuce and lettuce growth rate and lettuce growth rate calculation are the same as in Example 1. The results of this comparative example test are shown in Table 8.

[0098] Table 8 Influence of different processes on the performance of the prepared microbial soil conditioner

[0099] Process type Yield increase rate of lettuce Yield increase rate of lettuce lettuce Process of the present invention 204.15％ 218.56％ Comparative process 1 108.64％ 129.72％ Comparative process 2 79.38％ 64.97％

[0100] As can be seen from Table 8, the lettuce growth rate and lettuce growth rate achieved by the process of the present invention are significantly higher than those of Comparative Process 1 and Comparative Process 2, and higher than the sum of the two.

Claims

1. A method for synergistically utilizing phosphogypsum and phosphogypsum leachate to prepare a microbial soil conditioner, characterized in that, It includes the following steps: (1) Mix phosphogypsum leachate and white mud paste, stir evenly, dry, and activate at high temperature to obtain an activated material; the white mud paste is a mixture of white mud and phosphogypsum; (2) Mix sulfide slag, fly ash and the activated material in step (1), stir evenly, granulate, and age to obtain a material to be stabilized; (3) Mix papermaking stock preparation waste residue, mushroom residue and the material to be stabilized in step (2), stir evenly, carry out hydrothermal reaction, and after the reaction, the obtained mixed pulp is the activated pulp; (4) Mix the biomass probiotic liquid and the activated pulp in step (3), stir evenly, add compound bacteria for fermentation to obtain a microbial soil conditioner; the biomass probiotic liquid is a mixed liquid of blueberry juice, Rosa roxburghii raw pulp and concentrated apple juice; the compound bacteria are composed of Bacillus, Streptomyces, Saccobolus, Aspergillus; 2. The method according to claim 1, wherein In step (1), the mass ratio of the white mud to the phosphogypsum is 20-60:

100.

3. The method according to claim 1, wherein In step (1), the liquid-solid ratio of the phosphogypsum leachate to the white mud paste is 20-60:100 mL / g.

4. The method according to claim 1, characterized in that, In step (2), the mass ratio of the sulfide slag, fly ash and the activated material is 10-30:15-45:

100.

5. The method according to claim 1, wherein In step (3), the mass ratio of the papermaking stock preparation waste residue, mushroom residue and the material to be stabilized is 10-30:10-40:

100.

6. The method according to claim 1, wherein In step (4), the volume ratio of the blueberry juice, Rosa roxburghii raw pulp and concentrated apple juice is 5-15:5-15:

100.

7. The method according to claim 1, wherein In step (4), the mass ratio of the biomass probiotic liquid to the activated pulp is 2.5-7.5:

100.

8. The method according to claim 1, wherein In step (4), the Bacillus is any one of Bacillus firmus, Bacillus megaterium, Bacillus nieuwenhuizenii, Bacillus microflavus, Geobacillus stearothermophilus, Halobacillus salinus, Bacillus stamsii, Halobacillus salifodinae, Aerobacillus, Borobacillus, Bacillus subtilis, Paenibacillus borealis, Bacillus thuringiensis, Bacillus aryabhattai, Bacillus amyloliquefaciens, Bacillus sonorensis, Bacillus paralicheniformis, Bacillus nealsonii, Bacillus aryabhattai or Bacillus thiaminolyticus; the Streptomyces is any one of Streptomyces avermitilis, Streptomyces pactum, Streptomyces flavogriseus, Streptomyces nigrosporeus, Streptomyces thermoalkalophilus, Streptomyces albidoflavus, Streptomyces fradiae, Streptomyces vinaceusdrappus, Streptomyces herbarum, Streptomyces yogyakartensis, Streptomyces fengii, Streptomyces demanii, Streptomyces hiroshimensis, Streptomyces indicus, Streptomyces luteolus, Streptomyces cinnamoneus; the Saccobolus is any one of Saccobolus aberrans var. caucasicus, Saccobolus ater, Ashbya gossypii, Saccobolus altissimus, Saccobolus aberrans caucasicus or Ashbya multispora; the Aspergillus is any one of Aspergillus niger, Aspergillus sydowii, Aspergillus terreus, Aspergillus wentii, Aspergillus japonicus, Monascus ruber, Aspergillus usamii, Aspergillus oryzae, Aspergillus amstelodami, Aspergillus ficuum, Aspergillus carbonarius, Aspergillus nidulans or Monascus purpureus.

9. A microbial soil conditioner prepared by the method according to any one of claims 1-8.

10. Use of the microbial soil conditioner according to claim 9 in increasing plant yield.

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