Capillary electrophoresis apparatus

Abstract

The invention provides a capillary electrophoresis apparatus, the capillary electrophoresis apparatus is provided with a laser light source, an ultraviolet light source and a dichroscope, light emitted by the laser light source irradiates a transmission surface of the dichroscope, is transmitted by the dichroscope, and is focused to a transmission optical fiber through a second focusing convex lens, or is focused to a transmission optical fiber through a second focusing convex lens. Light emitted by the ultraviolet light source is converged into an integrated light beam through the second focusing convex lens, then the integrated light beam irradiates the reflecting surface of the dichroscope, is reflected by the dichroscope and then is focused to the transmission optical fiber through the second focusing convex lens, and then the light passes through the capillary tube and is transmitted to the photoelectric sensor through the receiving optical fiber. Compared with the prior art, detection of various substances with different absorption peaks can be achieved through one device, accessories do not need to be replaced, cost is lower, operation is easy and convenient, in addition, a plurality of photoelectric sensors are adopted, one optical fiber can be connected with one photoelectric sensor, detection is achieved based on light path signals, and detection sensitivity can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical detection, and particularly to a capillary electrophoresis instrument. Background Art

[0002] Capillary electrophoresis technology, also known as high-performance capillary electrophoresis, is a general term for a class of liquid-phase separation and analysis methods and techniques that use a capillary as the separation channel and a direct-current high-voltage electric field as the driving force, and are implemented according to the characteristics of the sample such as charge, size, isoelectric point, polarity, affinity behavior, and phase distribution.

[0003] A capillary electrophoresis instrument is one of the commonly used devices in biomedical detection. For example, when verifying the effect of biomedicine, the content of certain proteins in the human body can be detected by a capillary electrophoresis instrument, and the therapeutic effect can be verified according to the change in the protein content in the human body before and after taking the medicine.

[0004] However, the existing capillary electrophoresis instruments usually can only detect single or a small number of types of substances. For example, if different types of proteins need to be detected, relevant accessories need to be replaced, which is costly and cumbersome to operate. In addition, the existing capillary electrophoresis instruments usually use a CMOS sensor and realize detection based on the principle of image analysis. When performing multi-channel detection, multiple optical fibers are simultaneously connected to a CMOS sensor, and the detection sensitivity is relatively low. Summary of the Invention

[0005] Therefore, the purpose of the present invention is to provide a capillary electrophoresis instrument to solve the problems of high cost and cumbersome operation in the prior art when detecting multiple types of substances, and to improve the detection sensitivity.

[0006] A capillary electrophoresis instrument includes an optical path module and a core detection module; the optical path module includes an optical path bottom plate, and a UV light source assembly, a dichroic mirror assembly, a laser light source assembly, a focusing lens assembly, and a transmission optical fiber assembly mounted on the optical path bottom plate; The UV light source assembly includes a UV light source and a first focusing convex lens, and the first focusing convex lens is mounted on the outgoing light path of the UV light source; the dichroic mirror assembly includes a dichroic mirror; the laser light source assembly includes a laser light source; the focusing lens assembly includes a second focusing convex lens, and the transmission optical fiber assembly includes a plurality of transmission optical fibers; the dichroic mirror is fixed to the optical path bottom plate at a preset angle; the reflection surface of the dichroic mirror faces the UV light source, and the transmission surface of the dichroic mirror faces the laser light source; The core detection module includes a capillary bottom plate, a plurality of photoelectric sensors and a plurality of receiving optical fibers. The capillary bottom plate is provided with an optical fiber lower guide plate and a plurality of capillary assemblies. An anode cell and a cathode cell are provided at the bottom of the capillary bottom plate. The optical fiber lower guide plate is provided with a plurality of slots for placing transmission optical fibers. Each capillary assembly includes a capillary tube. One end of the capillary tube is inserted into the anode cell, and the other end is inserted into the cathode cell. The capillary tube is located between the transmission optical fiber and the receiving optical fiber. The receiving optical fiber is electrically connected to the photoelectric sensor; The light emitted by the laser light source irradiates the transmissive surface of the dichroic mirror. After being transmitted by the dichroic mirror, it is focused on the transmission optical fiber through the second focusing convex lens. After the light passes through the capillary tube, it is transmitted to the photoelectric sensor through the receiving optical fiber; Alternatively, the light emitted by the ultraviolet light source is converged into an integrated light beam by the first focusing convex lens, then irradiates the reflective surface of the dichroic mirror. After being reflected by the dichroic mirror, it is focused on the transmission optical fiber through the second focusing convex lens. After the light passes through the capillary tube, it is transmitted to the photoelectric sensor through the receiving optical fiber.

[0007] According to the capillary electrophoresis instrument provided by the present invention, due to the setting of the laser light source, the ultraviolet light source and the dichroic mirror, the light emitted by the laser light source irradiates the transmissive surface of the dichroic mirror. After being transmitted by the dichroic mirror, it is focused on the transmission optical fiber through the second focusing convex lens. Alternatively, the light emitted by the ultraviolet light source is converged into an integrated light beam by the second focusing convex lens, then irradiates the reflective surface of the dichroic mirror. After being reflected by the dichroic mirror, it is focused on the transmission optical fiber through the second focusing convex lens. Then the light passes through the capillary tube and is transmitted to the photoelectric sensor through the receiving optical fiber. When in use, only the corresponding light source needs to be selected according to the need, and the detection of multiple substances with different absorption peaks can be realized by one device without replacing accessories, with lower cost and simpler operation. In addition, the present invention adopts a plurality of photoelectric sensors. One optical fiber can be connected to one photoelectric sensor. The photoelectric sensor realizes detection based on the optical path signal, which can effectively improve the detection sensitivity. Description of the Drawings

[0008] Figure 1 is a schematic structural diagram of a capillary electrophoresis instrument according to an embodiment of the present invention; Figure 2 is a schematic structural diagram of the optical path module from the first perspective; Figure 3 is a schematic structural diagram of the optical path module from the second perspective; Figure 4 is an exploded structural diagram of the core detection module; Figure 5 is a schematic internal structure diagram of the core detection module; Figure 6 isFigure 5 An enlarged view of part A; Figure 7 is a schematic structural diagram of an optical fiber lower guide plate; Figure 8 is a schematic structural diagram of a capillary assembly; Figure 9 is a schematic structural diagram of a capillary groove plate, a capillary, and a fixing assembly; Figure 10 is a schematic structural diagram of the fixing assembly; Figure 11 is a schematic structural diagram of a first capillary fixing piece, a second optical fiber fixing seat, a mounting base, and a capillary; Figure 12 is a schematic structural diagram of a photoelectric sensor. Detailed implementation manners

[0009] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following will describe in detail the specific implementation manners of the present invention with reference to the accompanying drawings. Several embodiments of the present invention are shown in the accompanying drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0010] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there can also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right", "up", "down", and similar expressions used herein are only for the purpose of illustration and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0011] In the present invention, unless otherwise clearly defined and limited, the terms "installed", "connected", "connected to", "fixed", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0012] Please refer to Figures 1 to 12 , a capillary electrophoresis instrument provided by an embodiment of the present invention includes an optical path module 10 and a core detection module 20.

[0013] The optical path module 10 includes an optical path base plate 11, and a UV light source assembly 12, a dichroic mirror assembly 13, a laser light source assembly 14, a focusing lens assembly 15, and a transmission optical fiber assembly 16 mounted on the optical path base plate 11.

[0014] The UV light source assembly 12 includes a UV light source 121, a first focusing convex lens 122, a UV light outer sleeve 123, a UV light adjusting sleeve 124, a violet light adjusting fixing block 125, a slider 126, and a slide rail 127.

[0015] The UV light source 121 is installed in the UV light outer sleeve 123. In this embodiment, the UV light source 121 is a 280 nm UV light source, and the first focusing convex lens 122 is installed on the outgoing light path of the UV light source 121.

[0016] The UV light outer sleeve 123 is installed in the UV light adjusting sleeve 124, and the UV light adjusting sleeve 124 is installed in a through hole on the violet light adjusting fixing block 125 through adjusting bolts. Specifically, adjusting bolts distributed in a four-way central symmetry are used to adjust the angle of the UV light.

[0017] The violet light adjusting fixing block 125 is fixed to the slider 126, the slider 126 is installed on the slide rail 127, the slide rail 127 is fixed on the optical path base plate 11, and the slider 126 can slide on the slide rail 127. By sliding the slide rail 127, the focusing position of the UV light can be adjusted.

[0018] The dichroic mirror assembly 13 includes a dichroic mirror 131 and a dichroic mirror fixing block 132. The dichroic mirror 131 is installed on the dichroic mirror fixing block 132, and the dichroic mirror fixing block 132 is installed on the optical path base plate 11.

[0019] The laser light source assembly 14 includes a laser light source 141, a laser adjusting sleeve 142, and a laser adjusting fixing block 143. The laser light source 141 is installed in the laser adjusting sleeve 142. In this embodiment, the laser light source 141 is a 577 nm laser light source. A dual excitation system is formed by the 577 nm laser light source and the 280 nm UV light source.

[0020] The laser adjusting sleeve 142 is installed on the laser adjusting fixing block 143 through adjusting bolts, and adjusting bolts distributed in a four-way central symmetry are also used to adjust the laser angle.

[0021] The laser adjusting fixing block 143 is fixed on the optical path base plate 11.

[0022] The focusing lens assembly 15 includes a second focusing convex lens 151 and a focusing lens fixing block 152. The second focusing convex lens 151 is installed in the focusing lens fixing block 152, and the focusing lens fixing block 152 is fixed on the optical path bottom plate 11.

[0023] The transmission optical fiber assembly 16 includes a plurality of transmission optical fibers 161, an optical fiber fixing silica gel sleeve 162, and a transmission optical fiber fixing block 163. The transmission optical fibers 161 are installed in the optical fiber fixing silica gel sleeve 162, the optical fiber fixing silica gel sleeve 162 is installed on the transmission optical fiber fixing block 163, and the transmission optical fiber fixing block 163 is fixed on the optical path bottom plate 11.

[0024] The dichroic mirror 131 is fixed on the optical path bottom plate 11 at a preset angle; the reflecting surface of the dichroic mirror 131 faces the ultraviolet light source 121, and the transmitting surface of the dichroic mirror 131 faces the laser light source 141. The dichroic mirror 131 is composed of multiple layers of thin films. The thickness and refractive index of each layer of thin film are pre-designed so that at a specific wavelength, the reflected and transmitted light can meet a specific ratio. The dichroic mirror 131 used in this embodiment can transmit 577nm laser light and reflect 280nm ultraviolet light.

[0025] The core detection module 20 includes a capillary bottom plate 21, a plurality of receiving optical fibers 22, and a plurality of photoelectric sensors 23.

[0026] The capillary bottom plate 21 is provided with an optical fiber lower guide plate 24 and a plurality of capillary assemblies 25.

[0027] The bottom of the capillary bottom plate 21 is provided with an anode cell 261 and a cathode cell 262. The optical fiber lower guide plate 24 is provided with a plurality of slots 241 for placing the transmission optical fibers 161. Each capillary assembly 25 includes a capillary 251. One end of the capillary 251 is inserted into the anode cell 261, and the other end is inserted into the cathode cell 262. The anode cell 261 and the cathode cell 262 are used for electrophoresis of the sample solution in the capillary 251. The capillary 251 is located between the transmission optical fiber 161 and the receiving optical fiber 22, and the receiving optical fiber 22 is electrically connected to the photoelectric sensor 23.

[0028] The number of the transmission optical fibers 161, the number of the receiving optical fibers 22, and the number of the photoelectric sensors 23 are equal. The number of the transmission optical fibers 161 is one more than the number of the capillary tubes 251. One of the transmission optical fibers serves as a reference optical fiber to eliminate the detection error caused by environmental interference, and the remaining transmission optical fibers are respectively arranged in one-to-one correspondence with the capillary tubes. Specifically, in this embodiment, the number of the transmission optical fibers 161, the number of the receiving optical fibers 22, and the number of the photoelectric sensors 23 are all 9, and the number of the capillary tubes 251 is 8, that is, the number of the capillary tube assemblies 25 is 8. The 9 transmission optical fibers 161 are centrally constrained and fixed in the optical fiber fixing silica gel sleeve 162 to receive the coupled light beam.

[0029] The light emitted by the laser light source 141 irradiates the transmission surface of the dichroic mirror 131. After being transmitted by the dichroic mirror 131, it is focused on the transmission optical fiber 161 through the second focusing convex lens 151. After the light passes through the capillary tube 251, it is transmitted to the photoelectric sensor 23 through the receiving optical fiber 22, and the photoelectric sensor 23 converts the optical signal into an electrical signal and outputs the detection information. Alternatively, the light emitted by the ultraviolet light source 121 is converged into an integrated light beam by the first focusing convex lens 122, then irradiates the reflection surface of the dichroic mirror 131. After being reflected by the dichroic mirror 131, it is focused on the transmission optical fiber 161 through the second focusing convex lens 151. After the light passes through the capillary tube 251, it is transmitted to the photoelectric sensor 23 through the receiving optical fiber 22, and the photoelectric sensor 23 converts the optical signal into an electrical signal and outputs the detection information. Thus, the detection output of multiple substances (such as proteins) with different absorption peaks can be realized.

[0030] In this embodiment, the photoelectric sensor 23 adopts an S12698 silicon photodiode, which has high sensitivity and fast response. The 9 photoelectric sensors 23 are installed in the photoelectric sensor box 231.

[0031] Specifically, the core detection module 20 further includes a cooling upper cover 27, an optical fiber side end cover 281, a non-optical fiber side end cover 282, and a board cover 29.

[0032] The cooling upper cover 27 is installed on the capillary tube bottom plate 21. The optical fiber lower guiding plate 24 and the capillary tube assembly 25 are located inside the cooling upper cover 27. The cooling upper cover 27 is made of a heat-conducting material, and its inner surface is arc-shaped and covers the capillary tube 251, being in direct contact with the capillary tube 251, and is used to maintain the temperature stability of the capillary tube 251 during detection.

[0033] The fiber optic side end cap 281 and the non-fiber optic side end cap 282 are installed on both sides of the capillary bottom plate 21. A notch 2811 for the transmission optical fiber 161 to pass through is provided at the bottom of the fiber optic side end cap 281. The plate cover 29 is installed on the fiber optic lower guide plate 24 to fix the transmission optical fiber 161 in the fixing slot 241.

[0034] In this embodiment, the capillary 251 is semi-circular, and the slot 241 is L-shaped.

[0035] The capillary assembly 25 further includes a capillary groove plate 252, a capillary flat plate 253, and a fixing assembly 254. Both the capillary groove plate 252 and the capillary flat plate 253 are semi-circular. The capillary 251 is located between the capillary groove plate 252 and the capillary flat plate 253, and the capillary 251 is embedded and fixed in the groove of the capillary groove plate 252. The capillary 251 is fixed by the capillary groove plate 252 and the capillary flat plate 253.

[0036] A fixing assembly 254 is installed at each end of the capillary groove plate 252, and both ends of the capillary 251 are respectively located in the fixing assemblies 254 at both ends. The fixing assembly 254 is the core component for realizing detection and is used to connect the transmission optical fiber 161, the capillary 251, and the receiving optical fiber 22.

[0037] Specifically, the fixing assembly 254 includes a first optical fiber fixing seat 2541, a second optical fiber fixing seat 2542, a first capillary fixing piece 2543, and a second capillary fixing piece 2544. The first optical fiber fixing seat 2541 and the second optical fiber fixing seat 2542 are fixed together, and the two can be connected by bolts. The interiors of the first optical fiber fixing seat 2541 and the second optical fiber fixing seat 2542 are provided with optical fiber holes 2545, and the optical fiber holes 2545 intersect the capillary 251 at right angles. The first capillary fixing piece 2543 is installed in the first optical fiber fixing seat 2541, and the second capillary fixing piece 2544 is installed in the second optical fiber fixing seat 2542. The first capillary fixing piece 2543 and the second capillary fixing piece 2544 can be adhesively embedded in the fixing assembly 254.

[0038] The first capillary fixing piece 2543 and the second capillary fixing piece 2544 divide the optical fiber hole 2545 into a transmission optical fiber hole and a receiving optical fiber hole. The transmission optical fiber hole is used to accommodate the transmission optical fiber 161, and the receiving optical fiber hole is used to accommodate the receiving optical fiber 22.

[0039] In this embodiment, the first capillary fixing piece 2543 and the second capillary fixing piece 2544 are used to clamp the capillary 251, control the position of the capillary 251, and prevent the capillary 251 from being misaligned with the transmission optical fiber 161 and the receiving optical fiber 22.

[0040] In the prior art, if it is necessary to align the capillary with the transmission optical fiber and the receiving optical fiber, it is usually necessary to use a customized capillary to achieve this, resulting in a relatively high cost. In this embodiment, through the clamping of the capillary 251 by the first capillary fixing piece 2543 and the second capillary fixing piece 2544 in the fixing assembly 254, the position of the capillary 251 can be controlled to ensure the alignment of the capillary 251 with the transmission optical fiber 161 and the receiving optical fiber 22, without the need to use a customized capillary, thus achieving a lower cost.

[0041] In addition, in this embodiment, the core detection module 20 further includes a first optical fiber pressing base plate 201 and a second optical fiber pressing base plate 202, which are located on both sides of the fixing assembly 254, and the first optical fiber pressing base plate 201 and the second optical fiber pressing base plate 202 are provided with optical fiber grooves. The optical fiber groove on the first optical fiber pressing base plate 201 is used to place the transmission optical fiber 161, and the optical fiber groove on the second optical fiber pressing base plate 202 is used to place the receiving optical fiber 22, further ensuring the alignment of the capillary 251 with the transmission optical fiber 161 and the receiving optical fiber 22.

[0042] In this embodiment, the fixing assembly 254 further includes a mounting base 2546, which is mounted on the capillary base plate 21, and the mounting base 2546 is arranged corresponding to the anode cell 261 or the cathode cell 262. The first optical fiber fixing seat 2541 and the second optical fiber fixing seat 2542 are mounted on the mounting base 2546, and a sealing ring 2547 is provided on the outer periphery of the mounting base 2546. The sealing ring 2547 forms a seal with the anode cell 261 or the cathode cell 262 to prevent the sample solution from leaking.

[0043] In summary, according to the capillary electrophoresis instrument provided by the present invention, due to the provision of the laser light source 141, the ultraviolet light source 121 and the dichroic mirror 131, the light emitted by the laser light source 141 irradiates the transmission surface of the dichroic mirror 131, and after being transmitted by the dichroic mirror 131, it is focused on the transmission optical fiber 161 through the second focusing convex lens 151. Or, after the light emitted by the ultraviolet light source 121 is converged into an integrated light beam by the second focusing convex lens 151, it irradiates the reflection surface of the dichroic mirror 131, and after being reflected by the dichroic mirror 131, it is focused on the transmission optical fiber 161 through the second focusing convex lens 151. Then the light passes through the capillary 251 and is transmitted to the photoelectric sensor 23 through the receiving optical fiber 22. During use, only by selecting the corresponding light source as needed, the detection of multiple substances with different absorption peaks can be achieved through one device without replacing accessories, with a lower cost and simpler operation. In addition, the present invention uses multiple photoelectric sensors, and one optical fiber can be connected to one photoelectric sensor, and the photoelectric sensor realizes detection based on the optical path signal, which can effectively improve the detection sensitivity.

[0044] In the description of this specification, the descriptions referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc., mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0045] The above-described embodiments only express several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but should not be construed as limiting the protection scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A capillary electrophoresis instrument, characterized in that, It includes an optical path module and a core detection module; the optical path module includes an optical path base plate, and a UV light source assembly, a dichroic mirror assembly, a laser light source assembly, a focusing lens assembly, and a transmission optical fiber assembly mounted on the optical path base plate; The UV light source assembly includes a UV light source and a first focusing convex lens, and the first focusing convex lens is mounted on the outgoing light path of the UV light source; the dichroic mirror assembly includes a dichroic mirror; the laser light source assembly includes a laser light source; the focusing lens assembly includes a second focusing convex lens, and the transmission optical fiber assembly includes a plurality of transmission optical fibers; the dichroic mirror is fixed to the optical path base plate at a preset angle; the reflecting surface of the dichroic mirror faces the UV light source, and the transmitting surface of the dichroic mirror faces the laser light source; The core detection module includes a capillary base plate, a plurality of photoelectric sensors, and a plurality of receiving optical fibers. The capillary base plate is provided with an optical fiber lower guiding plate and a plurality of capillary assemblies. An anode cell and a cathode cell are provided at the bottom of the capillary base plate. The optical fiber lower guiding plate is provided with a plurality of slots for placing the transmission optical fibers. Each capillary assembly includes a capillary. One end of the capillary is inserted into the anode cell, and the other end is inserted into the cathode cell. The capillary is located between the transmission optical fiber and the receiving optical fiber, and the receiving optical fiber is electrically connected to the photoelectric sensor; The light emitted by the laser light source irradiates the transmitting surface of the dichroic mirror. After being transmitted by the dichroic mirror, it is focused on the transmission optical fiber through the second focusing convex lens. After the light passes through the capillary, it is transmitted to the photoelectric sensor through the receiving optical fiber; Alternatively, the light emitted by the UV light source is converged into an integrated light beam by the first focusing convex lens, then irradiates the reflecting surface of the dichroic mirror. After being reflected by the dichroic mirror, it is focused on the transmission optical fiber through the second focusing convex lens. After the light passes through the capillary, it is transmitted to the photoelectric sensor through the receiving optical fiber.

2. The capillary electrophoresis instrument according to claim 1, wherein The UV light source assembly further includes a UV light outer sleeve, a UV light adjusting sleeve, a violet light adjusting fixing block, a slider, and a slide rail. The UV light source is installed in the UV light outer sleeve, the UV light outer sleeve is installed in the UV light adjusting sleeve, the UV light adjusting sleeve is installed in a through hole on the violet light adjusting fixing block through an adjusting bolt, the violet light adjusting fixing block is fixed to the slider, the slider is installed on the slide rail, the slide rail is fixed to the optical path base plate, and the slider can slide on the slide rail.

3. The capillary electrophoresis instrument according to claim 2, wherein The dichroic mirror assembly further includes a dichroic mirror fixing block. The dichroic mirror is installed on the dichroic mirror fixing block, and the dichroic mirror fixing block is installed on the optical path base plate; The laser light source assembly further includes a laser adjusting sleeve and a laser adjusting fixing block. The laser light source is installed in the laser adjusting sleeve, the laser adjusting sleeve is installed on the laser adjusting fixing block through an adjusting bolt, and the laser adjusting fixing block is fixed to the optical path base plate; The focusing lens assembly further includes a focusing lens fixing block, the second focusing convex lens is installed in the focusing lens fixing block, and the focusing lens fixing block is fixed on the optical path bottom plate; The transmission optical fiber assembly further includes an optical fiber fixing silica gel sleeve and a transmission optical fiber fixing block, the transmission optical fiber is installed in the optical fiber fixing silica gel sleeve, the optical fiber fixing silica gel sleeve is installed on the transmission optical fiber fixing block, and the transmission optical fiber fixing block is fixed on the optical path bottom plate.

4. The capillary electrophoresis instrument according to claim 1, characterized in that, The core detection module further includes a cooling upper cover, an optical fiber side end cover, a non-optical fiber side end cover, and a plate cover; The cooling upper cover is installed on the capillary bottom plate, the optical fiber lower guide plate and the capillary assembly are located inside the cooling upper cover, the optical fiber side end cover and the non-optical fiber side end cover are installed on both sides of the capillary bottom plate, a notch for the transmission optical fiber to pass through is provided at the bottom of the optical fiber side end cover, and the plate cover is installed on the optical fiber lower guide plate.

5. The capillary electrophoresis instrument according to claim 4, characterized in that, The capillary is semicircular, and the slot hole is L-shaped; the capillary assembly further includes a capillary groove plate, a capillary flat plate and a fixing component. Both the capillary groove plate and the capillary flat plate are semicircular. The capillary is located between the capillary groove plate and the capillary flat plate, and the capillary is embedded and fixed in the groove of the capillary groove plate. The fixing components are respectively installed at both ends of the capillary groove plate, and both ends of the capillary are respectively located in the fixing components at both ends.

6. The capillary electrophoresis instrument according to claim 5, wherein The fixing component includes a first optical fiber fixing seat, a second optical fiber fixing seat, a first capillary fixing piece, and a second capillary fixing piece. The first optical fiber fixing seat and the second optical fiber fixing seat are fixed together, and optical fiber holes are provided inside the first optical fiber fixing seat and the second optical fiber fixing seat. The optical fiber holes intersect the capillary at right angles. The first capillary fixing piece is installed in the first optical fiber fixing seat, the second capillary fixing piece is installed in the second optical fiber fixing seat. The first capillary fixing piece and the second capillary fixing piece are used to clamp the capillary. The first capillary fixing piece and the second capillary fixing piece divide the optical fiber hole into a transmission optical fiber hole and a receiving optical fiber hole. The transmission optical fiber hole is used to accommodate the transmission optical fiber, and the receiving optical fiber hole is used to accommodate the receiving optical fiber.

7. The capillary electrophoresis instrument according to claim 6, characterized in that, The fixing component further includes a mounting base, the mounting base is installed on the capillary bottom plate, the mounting base is arranged corresponding to the anode cell or the cathode cell, the first optical fiber fixing seat and the second optical fiber fixing seat are installed on the mounting base, and a sealing ring is provided on the outer periphery of the mounting base.

8. The capillary electrophoresis instrument according to claim 5, wherein The core detection module further includes a first optical fiber pressing bottom plate and a second optical fiber pressing bottom plate. The first optical fiber pressing bottom plate and the second optical fiber pressing bottom plate are located on both sides of the fixing component, and the first optical fiber pressing bottom plate and the second optical fiber pressing bottom plate are provided with optical fiber grooves.

9. The capillary electrophoresis instrument according to claim 1, wherein The number of the transmission optical fibers and the receiving optical fibers is equal to the number of the photoelectric sensors. The number of the transmission optical fibers is one more than the number of the capillaries. One of the transmission optical fibers is used as a reference optical fiber, and the remaining transmission optical fibers are respectively arranged in one-to-one correspondence with the capillaries.

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