Culture medium for cultivating stropharia rugoso-annulata as well as preparation method and application of culture medium

Abstract

The invention relates to the technical field of edible mushroom cultivation, in particular to a culture medium for cultivating stropharia rugoso-annulata and a preparation method and application thereof. The culture medium for cultivating the stropharia rugoso-annulata is prepared from the following components: rape straw, soybean meal, rice chaff, pepper straw, rice straw, loofah sponge, livestock and poultry manure, corncob, sawdust, calcium lignosulphonate and gypsum powder. The time from sowing to fruiting is 40-55 days by adopting the stropharia rugoso-annulata culture medium provided by the invention, and the fruiting time is obviously shortened compared with that of a contrast. By adopting the culture medium for cultivating the stropharia rugoso-annulata, the average yield of the stropharia rugoso-annulata reaches 8.15-8.64 kg / m < 2 >, and compared with a contrast, the average yield of the stropharia rugoso-annulata is remarkably improved. Therefore, the oilseed rape straw, the soybean meal, the rice chaff, the pepper straw, the rice straw, the loofah sponge and the calcium lignosulphonate are compounded, the fruiting time of the stropharia rugoso-annulata is shortened, and the yield of the stropharia rugoso-annulata is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of edible mushroom cultivation, and particularly to a culture medium for cultivating Stropharia rugosoannulata, a preparation method thereof, and an application thereof. Background Art

[0002] Stropharia rugosoannulata ( Stropharia rugosoannulata ) is also known as Stropharia vinaceoavellanea and Stropharia rugoso-annulata f. brunneoviolacea, and belongs to Basidiomycota, Hymenomycetes, Agaricales, Strophariaceae, Stropharia. Stropharia rugosoannulata is a rare edible mushroom with good taste, high protein and low fat, and has health care functions. The fruiting body of Stropharia rugosoannulata has bright color, thick legs and fat caps, has a delicate fragrance, smooth and tender flesh, crispy stalks, rich nutrition, excellent taste, and makes people remember deeply after eating. The dried mushroom has a strong fragrance, is rich in quite high protein, various mineral elements and vitamins beneficial to the human body, and can treat or improve various diseases of the human body. It can be called a whole-price nutritious health food with delicious taste and "the meat among vegetables". Stropharia rugosoannulata is very popular whether it is stir-fried, fried, used for making soup, or used for hot pot. Stropharia rugosoannulata also has the effects of preventing coronary heart disease, helping digestion, and relieving mental fatigue of the human body, and is very popular in domestic and foreign markets. Moreover, the polysaccharide of Stropharia rugosoannulata has good antioxidant effects, can effectively scavenge free radicals, and has a significant improvement effect on the blood and liver of mice with oxidative damage caused by D-galactose. In addition, in addition to its edible and medicinal values, Stropharia rugosoannulata also has a strong ability to degrade lignin and cellulose. Manganese peroxidase, lignin peroxidase and laccase play key roles in the lignin degradation process.

[0003] At present, there is little research on the cultivation technology of Stropharia rugosoannulata, and it is necessary to find a method to improve the yield of Stropharia rugosoannulata to meet the market demand. Summary of the Invention

[0004] In order to solve the above problems, the present invention provides a culture medium for cultivating Stropharia rugosoannulata, a preparation method thereof, and an application thereof. The culture medium for cultivating Stropharia rugosoannulata provided by the present invention improves the yield of Stropharia rugosoannulata.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: The present invention provides a culture medium for cultivating Stropharia rugosoannulata, which is prepared from components including the following parts by weight: 20-30 parts of rape straw, 15-25 parts of soybean meal, 15-25 parts of rice bran, 20-30 parts of pepper straw, 15-25 parts of rice straw, 10-20 parts of loofah sponge, 5-10 parts of livestock and poultry manure, 5-10 parts of corn cob, 15-25 parts of wood chips, 2-8 parts of calcium lignosulfonate, and 1-5 parts of gypsum powder.

[0006] Preferably, it is prepared from the following components in parts by weight: 25 parts of rape straw, 20 parts of soybean meal, 20 parts of rice bran, 20 parts of pepper straw, 25 parts of rice straw, 18 parts of loofah sponge, 10 parts of livestock and poultry manure, 8 parts of corncob, 20 parts of wood chips, 5 parts of calcium lignosulfonate, and 5 parts of gypsum powder.

[0007] Preferably, the livestock and poultry manure includes one or more of naturally decomposed cow dung, sheep dung, and chicken dung; The wood chips include one or more of birch wood chips, Mongolian oak chips, and poplar wood chips.

[0008] The present invention also provides a method for preparing the culture medium for cultivating Stropharia rugosoannulata according to the above technical solution, which includes the following steps: 1) Mix the rape straw, soybean meal, rice bran, pepper straw, rice straw, loofah sponge, livestock and poultry manure, corncob, wood chips, calcium lignosulfonate, and gypsum powder, and adjust the water content to 60-70% to obtain the material to be fermented; 2) Naturally ferment the material to be fermented obtained in step 1), and turn the pile 2-3 times during the period to obtain the culture medium for cultivating Stropharia rugosoannulata.

[0009] The present invention also provides the application of the culture medium for cultivating Stropharia rugosoannulata according to the above technical solution in cultivating Stropharia rugosoannulata.

[0010] Preferably, the application includes the following steps: A. Inoculate the mother strain of Stropharia rugosoannulata in a liquid spawn medium and culture to obtain liquid spawn; B. Inoculate the liquid spawn obtained in step A in a solid spawn medium and culture until the mycelium fills it to obtain the cultivated spawn; the solid spawn medium is obtained by adding agar to the liquid spawn medium; C. Spread the culture medium for cultivating Stropharia rugosoannulata flat, sow the cultivated spawn obtained in step B, then cover the culture medium for cultivating Stropharia rugosoannulata, and then cover the soil with a thickness of 2-4 cm; D. Adopt conventional mycelium growth and fruiting management.

[0011] Preferably, the preparation method of the liquid spawn medium in step A includes: Mix peeled potatoes with water, boil for 30 min, and filter to obtain potato leachate; the mass ratio of the peeled potatoes to the volume of water is 200 g:1 L; Mix the potato leachate with corn flour, glucose, and potassium dihydrogen phosphate, and sterilize at 121 °C for 20-30 min to obtain the liquid spawn medium; The volume ratio of the potato leachate to the mass ratio of corn flour, glucose, and potassium dihydrogen phosphate is 1 L:60 g:20 g:4 g.

[0012] Preferably, the conditions for culturing in step A include: culturing in the dark at a temperature of 24-26°C and a rotation speed of 100-150 rpm for 5 days.

[0013] Preferably, the conditions for culturing in step B include: culturing in the dark at a temperature of 24-26°C for 20-25 days.

[0014] Preferably, the spreading amount of the Stropharia rugoso-annulata cultivation medium in step C is 15-20 kg / m 2 ; the covering thickness of the Stropharia rugoso-annulata cultivation medium is 8-10 cm; the seeding amount of the cultivated strains is 800-1000 g / m 2 , and the seeding is carried out by spot sowing in a plum blossom shape with a spacing of 10-12 cm.

[0015] Advantages of the present invention: Using the Stropharia rugoso-annulata cultivation medium provided by the present invention, the time from seeding to fruiting is 40-55 days, which is significantly shorter than that of the comparative example. Using the Stropharia rugoso-annulata cultivation medium provided by the present invention, the average yield of Stropharia rugoso-annulata reaches 8.15-8.64 kg / m 2 , which is significantly higher than the average yield of Stropharia rugoso-annulata in the comparative example. It can be shown from this that the present invention combines rape straw, pepper straw, rice straw, loofah sponge and calcium lignosulfonate, shortens the fruiting time of Stropharia rugoso-annulata, and improves the yield of Stropharia rugoso-annulata. Description of the drawings

[0016] Figure 1 It is the cultivated Stropharia rugoso-annulata. Detailed implementation manners

[0017] The present invention provides a Stropharia rugoso-annulata cultivation medium, which is prepared from components including the following parts by weight: 20-30 parts of rape straw, 15-25 parts of soybean meal, 15-25 parts of rice bran, 20-30 parts of pepper straw, 15-25 parts of rice straw, 10-20 parts of loofah sponge, 5-10 parts of livestock and poultry manure, 5-10 parts of corn cob, 15-25 parts of wood chips, 2-8 parts of calcium lignosulfonate and 1-5 parts of gypsum powder. In the present invention, the Stropharia rugoso-annulata cultivation medium is preferably prepared from components including the following parts by weight: 25 parts of rape straw, 20 parts of soybean meal, 20 parts of rice bran, 20 parts of pepper straw, 25 parts of rice straw, 18 parts of loofah sponge, 10 parts of livestock and poultry manure, 8 parts of corn cob, 20 parts of wood chips, 5 parts of calcium lignosulfonate and 5 parts of gypsum powder. In the present invention, the livestock and poultry manure preferably includes one or more of naturally decomposed cow dung, sheep dung and chicken dung; the wood chips include one or more of birch wood chips, Mongolian oak chips and poplar wood chips.

[0018] The present invention also provides a method for preparing a culture medium for cultivating Stropharia rugosoannulata according to the above technical solution, comprising the following steps: 1) Mix the rape straw, soybean meal, rice bran, pepper straw, rice straw, loofah sponge, livestock and poultry manure, corncob, sawdust, calcium lignosulfonate and gypsum powder, and adjust the water content to 60-70% to obtain a material to be fermented; 2) Naturally ferment the material to be fermented obtained in step 1), and turn the pile 2-3 times during the period to obtain a culture medium for cultivating Stropharia rugosoannulata.

[0019] The present invention mixes the rape straw, soybean meal, rice bran, pepper straw, rice straw, loofah sponge, livestock and poultry manure, corncob, sawdust, calcium lignosulfonate and gypsum powder, and adjusts the water content to 60-70% to obtain a material to be fermented. The present invention preferably crushes the rape straw, soybean meal, rice bran, pepper straw, rice straw, loofah sponge and corncob before mixing. The present invention has no special limitation on the mixing method, and conventional mixing can be adopted, such as stirring and mixing.

[0020] The present invention naturally ferments the obtained material to be fermented, and turns the pile 2-3 times during the period to obtain a culture medium for cultivating Stropharia rugosoannulata.

[0021] The present invention also provides an application of the culture medium for cultivating Stropharia rugosoannulata according to the above technical solution in cultivating Stropharia rugosoannulata.

[0022] In the present invention, the application preferably comprises the following steps: A. Inoculate the mother strain of Stropharia rugosoannulata in a liquid spawn medium for cultivation to obtain liquid spawn; B. Inoculate the liquid spawn obtained in step A in a solid spawn medium for cultivation until the mycelium fills the medium to obtain cultivated spawn; the solid spawn medium is obtained by adding agar to the liquid spawn medium; C. Spread the culture medium for cultivating Stropharia rugosoannulata, sow the cultivated spawn obtained in step B, then cover the culture medium for cultivating Stropharia rugosoannulata, and then cover the soil with a thickness of 2-4 cm; D. Adopt conventional mycelium growth and fruiting management.

[0023] In the present invention, the spawn of Stropharia rugoso-annulata is inoculated into a liquid spawn culture medium to obtain liquid spawn. There is no special limitation on the inoculation amount of the Stropharia rugoso-annulata spawn inoculated into the liquid spawn culture medium in the present invention, and those skilled in the art can adopt a conventional inoculation amount. There is no special limitation on the source of the Stropharia rugoso-annulata spawn in the present invention, and a conventional Stropharia rugoso-annulata spawn can be used. In the present invention, the preparation method of the liquid spawn culture medium preferably includes: mixing peeled potatoes with water, boiling for 30 min, and filtering to obtain a potato leachate; the mass ratio of the peeled potatoes to the volume of water is 200 g:1 L; mixing the potato leachate with corn flour, glucose, and potassium dihydrogen phosphate, and sterilizing at 121 °C for 15 - 20 min to obtain a liquid spawn culture medium; the volume ratio of the potato leachate to the mass of corn flour, glucose, and potassium dihydrogen phosphate is 1 L:60 g:20 g:4 g. In the present invention, the culture conditions preferably include: culturing in the dark at a temperature of 24 - 26 °C and a rotation speed of 100 - 150 rpm for 5 d.

[0024] In the present invention, the obtained liquid spawn is inoculated into a solid spawn culture medium for culture until the mycelium covers the medium completely to obtain a cultivation spawn; the solid spawn culture medium is obtained by adding agar to the liquid spawn culture medium. There is no special limitation on the inoculation amount of the liquid spawn inoculated into the solid spawn culture medium in the present invention, and those skilled in the art can adopt a conventional amount. In the present invention, the culture conditions preferably include: culturing in the dark at a temperature of 24 - 26 °C for 20 - 25 d.

[0025] In the present invention, the cultivation medium for Stropharia rugoso-annulata is laid flat, the obtained cultivation spawn is sown, then the cultivation medium for Stropharia rugoso-annulata is covered, and then 2 - 4 cm of soil is covered. In the present invention, the laying amount of the cultivation medium for Stropharia rugoso-annulata is preferably 15 - 20 kg / m 2 . In the present invention, the covering thickness of the cultivation medium for Stropharia rugoso-annulata is preferably 8 - 10 cm. In the present invention, the sowing amount of the cultivation spawn is preferably 800 - 1000 g / m 2 , and the sowing preferably adopts a plum blossom dot sowing method, and the spacing is preferably 10 - 12 cm.

[0026] In the present invention, conventional mycelium growth and fruiting management are adopted. In the present invention, the conventional mycelium growth preferably includes the following steps: the temperature is 24 - 26 °C, the humidity of the cultivation medium for Stropharia rugoso-annulata is 60 - 70%, the environmental humidity is 75 - 85%, and no light is required. In the present invention, the conventional fruiting management preferably includes the following steps: the temperature is 24 - 26 °C, the humidity of the cultivation medium for Stropharia rugoso-annulata is 65 - 75%, the environmental humidity is 90%, and sufficient scattered light is given.

[0027] To further illustrate the present invention, the present invention will be described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention. Embodiment

[0028] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 25 kg of rape straw, 20 kg of soybean meal, 20 kg of rice bran, 20 kg of pepper straw, 25 kg of rice straw, 18 kg of loofah sponge, 10 kg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, 5 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain a material to be fermented; among them, the rape straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are mixed after being crushed; 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain a culture medium for cultivating Stropharia rugoso-annulata. Embodiment

[0029] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 20 kg of rape straw, 20 kg of soybean meal, 20 kg of rice bran, 28 kg of pepper straw, 20 kg of rice straw, 10 kg of loofah sponge, 5 kg of naturally decomposed cow dung, 10 kg of corn cob, 15 kg of birch sawdust, 2 kg of calcium lignosulfonate, and 1 kg of gypsum powder, and adjust the water content to 60% to obtain a material to be fermented; among them, the rape straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are mixed after being crushed; 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain a culture medium for cultivating Stropharia rugoso-annulata. Embodiment

[0030] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 30 kg of rape straw, 20 kg of soybean meal, 20 kg of rice bran, 30 kg of pepper straw, 15 kg of rice straw, 10 kg of loofah sponge, 10 kg of naturally decomposed cow dung, 5 kg of corn cob, 15 kg of birch sawdust, 5 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain a material to be fermented; among them, the rape straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are mixed after being crushed; 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain a culture medium for cultivating Stropharia rugoso-annulata. Embodiment

[0031] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 20 kg of rapeseed straw, 20 kg of soybean meal, 20 kg of rice bran, 30 kg of pepper straw, 15 kg of rice straw, 20 kg of loofah sponge, 10 kg of naturally decomposed cow dung, 5 kg of corn cob, 16 kg of birch sawdust, 2 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented. Among them, the rapeseed straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are crushed and then mixed. 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata. Example

[0032] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata comprises the following steps: 1) Mix 22 kg of rapeseed straw, 20 kg of soybean meal, 20 kg of rice bran, 24 kg of pepper straw, 17 kg of rice straw, 15 kg of loofah sponge, 10 kg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, 8 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented. Among them, the rapeseed straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are crushed and then mixed. 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata. Example

[0033] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata comprises the following steps: 1) Mix 25 kg of rapeseed straw, 20 kg of soybean meal, 20 kg of rice bran, 20 kg of pepper straw, 15 kg of rice straw, 10 kg of loofah sponge, 10 kg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, 6 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented. Among them, the rapeseed straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are crushed and then mixed. 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata.

[0034] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata comprises the following steps: 1) Mix 18 kg of loofah sponge, 10 lg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, 5 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented. Among them, the loofah sponge, naturally decomposed cow dung, and corn cob are crushed and then mixed. 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata.

[0035] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 25 kg of rape straw, 20 kg of pepper straw, 25 kg of rice straw, 10 lg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, 5 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented; among them, the rape straw, pepper straw, rice straw, naturally decomposed cow dung, and corn cob are mixed after being crushed; 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata.

[0036] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 25 kg of rape straw, 20 kg of pepper straw, 25 kg of rice straw, 18 kg of loofah sponge, 10 lg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented; among them, the rape straw, pepper straw, rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are mixed after being crushed; 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata.

[0037] A method for preparing a culture medium for cultivating Stropharia rugoso-annulata, comprising the following steps: 1) Mix 25 kg of rice straw, 18 kg of loofah sponge, 10 lg of naturally decomposed cow dung, 8 kg of corn cob, 20 kg of birch sawdust, 5 kg of calcium lignosulfonate, and 5 kg of gypsum powder, and adjust the water content to 60% to obtain the material to be fermented; among them, the rice straw, loofah sponge, naturally decomposed cow dung, and corn cob are mixed after being crushed; 2) Naturally ferment the obtained material to be fermented, and turn the pile twice during the period to obtain the culture medium for cultivating Stropharia rugoso-annulata.

[0038] In 2024, Stropharia rugoso-annulata was cultivated using the culture media prepared in Examples 1 to 6 and Comparative Examples 1 to 4 in the experimental base of the Jilin Academy of Agricultural Sciences. Each treatment was 1 mu, with 3 replicates, and the average value was taken.

[0039] The cultivation method is as follows: 1. Inoculate the mother strain of Stropharia rugoso-annulata in a liquid spawn medium and culture to obtain liquid spawn; the culture conditions are: culturing in the dark at a temperature of 25 °C and a rotation speed of 100 rpm for 5 d; The preparation method of the liquid spawn medium is: Mix peeled potatoes with water, boil for 30 min, and filter to obtain potato leachate; the mass ratio of peeled potatoes to the volume of water is 200 g:1 L; Mix the potato leachate with corn flour, glucose, and potassium dihydrogen phosphate, and sterilize at 121 °C for 20 min to obtain a liquid spawn medium; the volume ratio of the potato leachate to the mass of corn flour, glucose, and potassium dihydrogen phosphate is 1 L: 60 g: 20 g: 4 g; 2. Inoculate the liquid spawn obtained in step 1 into a solid spawn medium and cultivate until the mycelium covers the medium to obtain the cultivated spawn; the solid spawn medium is obtained by adding 20 g of agar to each liter of the liquid spawn medium; the cultivation conditions are: cultivate in the dark at 25 °C for 25 d; 3. Spread the cultivated Stropharia rugoso-annulata media prepared in Examples 1-6 and Comparative Examples 1-4 respectively, sow the cultivated spawn obtained in step 2, then cover the cultivated Stropharia rugoso-annulata medium, and then cover with 4 cm of soil; Among them, the spreading amount of the cultivated Stropharia rugoso-annulata medium is 20 kg / m 2 ; the covering thickness of the cultivated Stropharia rugoso-annulata medium is 10 cm; the seeding amount of the cultivated spawn is 1000 g / m 2 , and the seeding is carried out by spot sowing in a plum blossom shape with a spacing of 10 cm; 4. Adopt conventional mycelium growth and fruiting management, specifically as follows: Mycelium growth management: The temperature is 25 °C, the humidity of the cultivated Stropharia rugoso-annulata medium is 65%, the environmental humidity is 80%, and no light is required; Fruiting management: The temperature is 25 °C, the humidity of the cultivated Stropharia rugoso-annulata medium is 75%, the environmental humidity is 90%, and sufficient scattered light is given.

[0040] Statistical analysis was performed on the time from sowing to fruiting, and the average yield was calculated. The results are shown in Table 1.

[0041] Table 1 Fruiting time and average yield Time from sowing to fruiting / d <![CDATA[Average yield kg / m 2 > Example 1 40 8.64 Example 2 48 8.25 Example 3 41 8.15 Example 4 55 8.34 Example 5 44 8.39 Example 6 42 8.22 Comparative Example 1 80 5.82 Comparative Example 2 74 6.92 Comparative Example 3 75 6.73 Comparative Example 4 82 4.74 As can be seen from Table 1, the time from sowing to fruiting of the cultivated Stropharia rugoso-annulata medium provided by the present invention is 40-55 d, which is significantly shorter than that of the comparative examples. Using the cultivated Stropharia rugoso-annulata medium provided by the present invention, the average yield of Stropharia rugoso-annulata reaches 8.15-8.64 kg / m 2 , which is significantly higher than that of the comparative examples. It can be seen that the present invention combines rape straw, soybean meal, rice bran, pepper straw, rice straw, loofah sponge, and calcium lignosulfonate, shortening the fruiting time of Stropharia rugoso-annulata and increasing the yield of Stropharia rugoso-annulata.

[0042] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A cultivation medium for Stropharia rugosoannulata, characterized in that, It is prepared from components including the following parts by weight: 20 - 30 parts of rape straw, 15 - 25 parts of soybean meal, 15 - 25 parts of rice bran, 20 - 30 parts of pepper straw, 15 - 25 parts of rice straw, 10 - 20 parts of loofah sponge, 5 - 10 parts of livestock and poultry manure, 5 - 10 parts of corncob, 15 - 25 parts of wood chips, 2 - 8 parts of calcium lignosulfonate, and 1 - 5 parts of gypsum powder.

2. The cultivated Stropharia rugoso-annulata culture medium according to claim 1, characterized in that, It is prepared from components including the following parts by weight: 25 parts of rape straw, 20 parts of soybean meal, 20 parts of rice bran, 20 parts of pepper straw, 25 parts of rice straw, 18 parts of loofah sponge, 10 parts of livestock and poultry manure, 8 parts of corncob, 20 parts of wood chips, 5 parts of calcium lignosulfonate, and 5 parts of gypsum powder.

3. The cultivation medium for Stropharia rugosoannulata according to claim 1 or 2, characterized in that, The livestock and poultry manure includes one or more of naturally decomposed cow dung, sheep dung, and chicken dung; The wood chips include one or more of birch wood chips, Mongolian oak wood chips, and poplar wood chips.

4. The preparation method of the cultivation medium for Stropharia rugosoannulata according to any one of claims 1 to 3, characterized in that, It includes the following steps: 1) Mix the rape straw, soybean meal, rice bran, pepper straw, rice straw, loofah sponge, livestock and poultry manure, corncob, wood chips, calcium lignosulfonate, and gypsum powder, and adjust the water content to 60 - 70% to obtain the material to be fermented; 2) Naturally ferment the material to be fermented obtained in step 1), and turn the pile 2 - 3 times during the period to obtain the culture medium for cultivating Stropharia rugoso - annulata.

5. Use of the culture medium for cultivating Stropharia rugoso - annulata according to any one of claims 1 - 3 in cultivating Stropharia rugoso - annulata.

6. The application according to claim 5, wherein The use includes the following steps: A. Inoculate the mother strain of Stropharia rugoso - annulata in a liquid spawn medium and cultivate to obtain liquid spawn; B. Inoculate the liquid spawn obtained in step A in a solid spawn medium and cultivate until the mycelium fills it to obtain the cultivation spawn; the solid spawn medium is obtained by adding agar to the liquid spawn medium; C. Spread the culture medium for cultivating Stropharia rugoso - annulata flat, sow the cultivation spawn obtained in step B, then cover the culture medium for cultivating Stropharia rugoso - annulata, and then cover the soil with a thickness of 2 - 4 cm; D. Adopt conventional mycelium growth and fruiting management.

7. The application according to claim 6, characterized in that, The preparation method of the liquid spawn medium in step A includes: Mix peeled potatoes with water, boil for 30 min, and filter to obtain potato leachate; the mass ratio of the peeled potatoes to the volume of water is 200 g:1 L; Mix the potato leachate with corn flour, glucose, and potassium dihydrogen phosphate, and sterilize at 121 °C for 20 - 30 min to obtain the liquid spawn medium; The volume ratio of the potato leachate to the mass of corn flour, glucose, and potassium dihydrogen phosphate is 1 L:60 g:20 g:4 g.

8. The application according to claim 6, wherein The cultivation conditions in step A include: Cultivate in the dark at a temperature of 24 - 26 °C and a rotation speed of 100 - 150 rpm for 5 d.

9. The application according to claim 6, characterized in that, The cultivation conditions in step B include: Cultivate in the dark at a temperature of 24 - 26 °C for 20 - 25 d.

10. The application according to claim 6, characterized in that, The spreading amount of the culture medium for cultivating Stropharia rugosoannulata in step C is 15-20 kg / m 2 ; The covering thickness of the culture medium for cultivating Stropharia rugoso - annulata is 8 - 10 cm; The seeding rate of the cultivated spawn is 800 - 1000 g / m 2 , and the seeding is carried out by spot sowing in a plum blossom pattern with a spacing of 10 - 12 cm.

Images