Method for improving drought resistance and promoting growth of crops by using 3-methylbutylamine

Abstract

The invention provides a method for improving crop drought resistance and promoting growth by using 3-methylbutylamine, and belongs to the technical field of plant drought resistance. The invention provides application of 3-methylbutylamine in improvement of crop drought resistance and promotion of growth, and discovers that 3-methylbutylamine can enhance the water retention ability of plants, improve the water absorption and regulation ability, improve the drought resistance of plants, enhance the oxidation resistance, relieve oxidative damage, protect plants and relieve damage of drought stress. The yield of crops can be increased, and the sustainable development of agriculture is promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of plant drought resistance, and particularly to a method for improving the drought resistance and promoting the growth of crops by using 3-methylbutylamine. Background Art

[0002] Drought is one of the most important abiotic stresses affecting global agricultural production. With the intensification of climate change, the frequency and intensity of droughts continue to increase, seriously threatening food security. Drought stress can lead to water deficit in crops, affecting photosynthesis, nutrient absorption, growth and development, and ultimately reducing yield and quality. For example, major food crops such as maize, wheat, and rice may result in a 30% - 50% reduction in yield when encountering drought during the critical growth period. Therefore, improving the drought resistance of crops has become an important direction in agricultural research.

[0003] Existing drought resistance methods mainly include plant body improvement, soil and water management, exogenous substance regulation, and microbial application, etc. Although these drought resistance methods are effective, they also have certain limitations. For example, plant body improvement (such as drought-resistant variety breeding) usually requires a long breeding cycle and high costs, and the adaptability of genetically modified varieties may be limited by specific environmental conditions. Soil and water management methods, such as drip irrigation and mulch cultivation, although they can improve water use efficiency, require high infrastructure investment and technical maintenance, and may not be fully applicable in areas with limited water resources. Microbial application, such as the use of mycorrhizal fungi and growth-promoting bacteria, depends on the stability of the soil ecological environment and may not be able to play a lasting role in some areas. In contrast, exogenous substance regulation has obvious advantages, especially exogenous spraying substances have the advantages of being fast, effective, and easy to operate in improving the drought resistance of crops. Osmotic regulators (such as proline, mannitol) can stabilize cell membranes and slow down water loss; reasonable use of these exogenous substances can significantly relieve drought stress and increase crop yield, but there are also problems such as high costs and limited field application. Therefore, developing an exogenous additive with low cost, convenient application, and capable of effectively regulating the water balance of plants, enhancing antioxidant capacity, and promoting root development, thereby improving the drought resistance and growth vitality of crops, helps to increase crop yield and promote the sustainable development of agriculture. Summary of the Invention

[0004] The purpose of the present invention is to provide an application of 3-methylbutylamine in improving the drought resistance and promoting the growth of crops. By applying 3-methylbutylamine, the physiological responses of plants are regulated, and the growth potential and stress resistance of crops under drought conditions are enhanced.

[0005] To achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0006] The present invention provides an application of 3-methylbutylamine in improving the drought resistance and promoting the growth of crops.

[0007] The present invention also provides a method for enhancing the drought resistance of crops and promoting growth by using 3-methylbutylamine, which is to mix 3-methylbutylamine with water and then spray it on the leaf surface of the crops.

[0008] Preferably, the concentration of the mixture of 3-methylbutylamine and water is 8-10 mmol / L.

[0009] Preferably, the spraying method is: during the seedling stage of the crops, spray once every 2-3 days for 10-15 times.

[0010] Preferably, the spraying amount is such that the leaf surface of the crops is evenly covered with water vapor and does not converge into drops and slide off.

[0011] Preferably, the crops include: soybean, wheat, corn, sorghum and flax.

[0012] 3-Methylbutylamine, also known as isoamylamine, is a chemical intermediate and is currently mainly used in aspects such as medicine, dyes, corrosion inhibitors, solvents, etc. The present invention first uses it as an exogenous substance regulator for crops.

[0013] The application of 3-methylbutylamine provided by the present invention in enhancing the drought resistance of crops and promoting growth can enhance the water retention ability of plants, improve the water absorption and regulation mechanism, and promote root development and water use efficiency by using 3-methylbutylamine. Especially in a drought environment, it can reduce the water loss of plants and improve the drought resistance of plants. It can also enhance the antioxidant ability of plants, reduce oxidative damage, and protect plant cells from the damage of drought stress. It can adjust the physiological state of plants under drought conditions, improve the growth ability and drought resistance of crops under drought stress, be applicable to agricultural production in arid and semi-arid regions, help increase crop yields, and promote the sustainable development of agriculture. Description of the Drawings

[0014] Figure 1 It is the random forest ranking diagram of metabolites under drought treatment in Example 1;

[0015] Figure 2 It is the result diagram of the changes in plant height and dry weight of corn in Example 2;

[0016] Figure 3 It is the result diagram of the changes in plant height and dry weight of soybean in Example 3;

[0017] Figure 4 It is the result diagram of the changes in plant height and dry weight of sorghum in Example 4;

[0018] Figure 5 It is the result diagram of the changes in plant height and dry weight of wheat in Example 5;

[0019] Figure 6It is a graph showing the changes in plant height and dry weight of flax in Example 6. Detailed implementation mode

[0020] The technical solutions provided by the present invention will be described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0021] Example 1

[0022] By performing random forest ranking analysis on the metabolites with significant differences in the roots of flax under drought treatment (severe drought: soil mass water content in the field is 6% - 8%), it was found that metabolite 3-methylbutylamine (A891) ranked 20th ( Figure 1 ), being the amine substance with the highest ranking. Therefore, we selected maize, soybean, sorghum, wheat, and flax crops as experimental materials for metabolite spraying verification experiments. When the crops grew to the seedling stage, the treatment group of crops was sprayed with a 10 mmol / L 3-methylbutylamine solution on the leaf surface once every 3 days, for a total of 10 times. The control group (CK) was sprayed with an equal amount of sterile water. After spraying, the plant phenotypes were observed and relevant indicators were measured to verify the role of 3-methylbutylamine in the drought resistance process of flax and other crops.

[0023] In the following examples, the 3-methylbutylamine solution was prepared by mixing sterile water and 3-methylbutylamine.

[0024] Example 2

[0025] Under drought conditions (moderate drought: soil water content is 40 - 45%), maize (1 plant per pot) was planted in pots. When the maize grew to the seedling stage, in the treatment group: the seedling-stage maize was sprayed with a 10 mmol / L 3-methylbutylamine solution once every 3 days until the leaf surface of the crop was evenly covered with water vapor and did not converge into drops and slide off, for a total of 10 times. In the control group: the same operation of spraying an equal amount of sterile water as in the treatment group. There were 20 plants in both the treatment group and the control group. The results are as Figure 2 shown.

[0026] From Figure 2 it can be seen that after measuring the plant height and dry weight, it was found that the average plant height of the maize in the treatment group was 79.90 cm, which was 12.60% higher than that of the control group (70.94 cm); the average dry weight of the maize in the treatment group was 2.23 g, which was 24.00% higher than that of the control group (1.80 g). These results indicate that spraying 3-methylbutylamine can significantly promote the growth and biomass accumulation of maize plants, thereby enhancing their adaptability to the drought environment.

[0027] Example 3

[0028] Under drought conditions (moderate drought: soil water content 40 - 45%), soybeans were planted in pots (1 plant per pot). After the soybeans grew to the seedling stage, in the treatment group: the seedling-stage soybeans were sprayed with a 10 mmol / L 3-methylbutylamine solution every 3 days until the leaves of the crop were evenly covered with water vapor and did not gather into drops and slide off, for a total of 10 times. In the control group: the same operation as the treatment group was carried out by spraying an equal amount of sterile water. There were 20 plants in both the treatment group and the control group. The results are as Figure 3 shown.

[0029] As Figure 3 can be seen, after measuring the plant height and dry weight, it was found that the average plant height of the soybeans in the treatment group was 64.74 cm, which was 8.90% higher than that of the control group (59.48 cm); the average dry weight of the soybeans in the treatment group was 0.57 g, which was 26.70% higher than that of the control group (0.45 g). These results indicate that spraying 3-methylbutylamine can significantly promote the growth and biomass accumulation of soybean plants, thereby enhancing their adaptability to the drought environment.

[0030] Example 4

[0031] Under drought conditions (moderate drought: soil water content 40 - 45%), sorghum was planted in pots (1 plant per pot). After the sorghum grew to the seedling stage, in the treatment group: the seedling-stage sorghum was sprayed with a 10 mmol / L 3-methylbutylamine solution every 3 days until the leaves of the crop were evenly covered with water vapor and did not gather into drops and slide off, for a total of 10 times. In the control group: the same operation as the treatment group was carried out by spraying an equal amount of sterile water. There were 20 plants in both the treatment group and the control group. The results are as Figure 4 shown.

[0032] As Figure 4 can be seen, after measuring the plant height and dry weight, it was found that the average plant height of the sorghum in the treatment group was 67.86 cm, which was 11.00% higher than that of the control group (61.13 cm); the average dry weight of the sorghum in the treatment group was 0.40 g, which was 17.50% higher than that of the control group (0.34 g). These results indicate that spraying 3-methylbutylamine can significantly promote the growth and biomass accumulation of sorghum plants, thereby enhancing their adaptability to the drought environment.

[0033] Example 5

[0034] Under drought conditions (moderate drought: soil water content 40 - 45%), wheat was planted in pots (1 plant per pot). After the wheat grew to the seedling stage, in the treatment group: the seedling-stage wheat was sprayed with a 10 mmol / L 3-methylbutylamine solution every 3 days until the leaves of the crop were evenly covered with water vapor and did not gather into drops and slide off, for a total of 10 times. In the control group: the same operation as the treatment group was carried out by spraying an equal amount of sterile water. There were 20 plants in both the treatment group and the control group. The results are as Figure 5 shown.

[0035] It can be seen from Figure 5 that after measuring the plant height and dry weight, it was found that the average plant height of wheat in the treatment group was 43.01 cm, which was 17.00% higher than that of 36.80 cm in the control group; the average dry weight of wheat in the treatment group was 0.34 g, which was 13.10% higher than that of 0.30 g in the control group. These results indicate that spraying 3-methylbutylamine can significantly promote the growth and biomass accumulation of wheat plants, thereby enhancing their adaptability to drought environment.

[0036] Example 6

[0037] Under drought conditions (moderate drought: soil water content 40 - 45%), flax was planted in pots (1 plant per pot). After the flax grew to the seedling stage, in the treatment group: the seedling-stage flax was sprayed with a 10 mmol / L 3-methylbutylamine solution every 3 days until the crop leaves were evenly covered with water vapor without gathering into drops and sliding off, for a total of 10 times. The control group was sprayed with an equal amount of sterile water in the same operation as the treatment group. There were 20 plants in both the treatment group and the control group. The results are as Figure 6 shown.

[0038] It can be seen from Figure 6 that after measuring the plant height and dry weight, it was found that the average plant height of flax in the treatment group was 34.62 cm, which was 19.10% higher than that of 29.06 cm in the control group; the average dry weight of flax in the treatment group was 0.37 g, which was 12.20% higher than that of 0.33 g in the control group. These results indicate that spraying 3-methylbutylamine can significantly promote the growth and biomass accumulation of flax plants, thereby enhancing their adaptability to drought environment.

[0039] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. Application of 3-methylbutylamine in enhancing drought resistance and promoting growth of crops.

2. A method for improving the drought resistance and promoting the growth of crops by using 3-methylbutylamine, characterized in that, Mix 3-methylbutylamine with water and spray it on the leaf surface of crops.

3. The method according to claim 2, wherein The concentration of the mixture of 3-methylbutylamine and water is 8 - 10 mmol / L.

4. The method according to claim 3, wherein The spraying method is as follows: during the seedling stage of crops, spray once every 2 - 3 days for 10 - 15 times.

5. The method according to claim 4, wherein The spraying amount is such that the leaf surface of the crops is evenly covered with water vapor without gathering into drops and sliding off.

6. The application according to claim 1 or the method according to any one of claims 2 to 5, characterized in that The crops include: soybean, wheat, corn, sorghum and flax.

Images