Bactericidal composition and application thereof

Abstract

The invention discloses a bactericidal composition and application thereof, the bactericidal composition contains an active component A and an active component B, the active component A is selected from pyrimidine nucleoside antibiotics, and the active component B is selected from any one of metalaxyl and salt, propamocarb and salt, fluazinam or active ester. The composition disclosed by the invention has relatively high activity on a plurality of diseases on a plurality of crops, and has an obvious synergistic effect, and the bactericidal spectrum is expanded. The composition has the characteristics of small dosage, rain wash resistance and obvious synergism.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides and relates to a bactericidal composition and its application. Background Art

[0002] Agricultural antiobiotic 120, also known as agricultural antibiotic 120, antimycin 120, and 120 agricultural antibiotic, is a broad-spectrum antibiotic. It has a strong inhibitory effect on many plant pathogens and has good control effects on powdery mildew of cucurbits, powdery mildew of flowers, and wheat rust.

[0003] Metalaxyl, chemical name: methyl D,L-N-(2,6-dimethylphenyl)-N-(2'-methoxyacetyl)alaninate, molecular formula: C 15 H 21 NO4. The salt of Metalaxyl refers to Metalaxyl-M, chemical name: methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-D-alaninate. Metalaxyl and its salt are systemic fungicides with protective and therapeutic effects. They can be absorbed by the roots, stems, and leaves of plants and transferred to various organs of the plants along with the water movement in the plants. They can be used for foliar treatment, seed treatment, and soil treatment, and are effective against diseases caused by downy mildew, Phytophthora, and Pythium.

[0004] Propamocarb, chemical name: propyl 3-(dimethylamino)propylcarbamate, molecular formula: C9H 21 N2O2Cl. The salt of Propamocarb refers to Propamocarb hydrochloride, chemical name: hydrochloride of propyl 3-(dimethylamino)propylcarbamate. Propamocarb and its salt have local systemic effects and can also be used for dipping treatment and seed protectant.

[0005] Fluazinam, chemical name: 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridyl)-α,α,α-trifluoro-2,6-dinitro-p-toluidine. This product belongs to 2,6-dinitroaniline compounds and is a protective fungicide. This product is very effective against Alternaria, Botrytis, Phytophthora, Plasmopara, Sclerotinia, and Phoma. It also has good effects on Botrytis cinerea resistant to benzimidazole and dicarboximide fungicides. It is resistant to rain erosion, has a long-lasting effect, and also has excellent control effects on phytophagous mites. It has excellent control effects on clubroot of cruciferous plants and also has good control effects on rice damping-off caused by Rhizopus.

[0006] Activated ester (Acibenzolar) Chemical name: Methyl 1,2,3-benzothiadiazole-7-thiocarboxylate, Molecular formula: C 21 H 16 ClF3N4O5. Activated ester is a plant disease resistance activator. It has no bactericidal activity and therapeutic effect itself, but after being absorbed by the plant, it activates the plant's resistance mechanism and its own defense resistance reaction, so that the plant has a broad-spectrum self-protection effect against a variety of fungi, bacteria, nematodes and diseases and produces long-lasting resistance. Summary of the Invention

[0007] The object of the present invention is to provide a bactericidal composition and its application to solve the problems existing in the above-mentioned prior art. Long-term use of antibiotics of pyrimidine nucleosides, metalaxyl and its salts, propamocarb and its salts, fluazinam, and activated ester single agents may bring problems such as resistance occurrence and reduced drug efficacy. The bactericidal composition proposed by the present invention contains active ingredient A and active ingredient B, as well as appropriate surfactants and carriers.

[0008] To achieve the above object, the present invention provides the following solutions:

[0009] One of the technical solutions of the present invention is a bactericidal composition containing active ingredient A and active ingredient B. The weight ratio of active ingredient A to active ingredient B is 1:80 to 60:1. The active ingredient A is selected from antibiotics of pyrimidine nucleosides, and the active ingredient B is selected from one of metalaxyl and its salts, propamocarb and its salts, fluazinam, and activated ester.

[0010] The preferable weight ratio of active ingredient A to active ingredient B is 1:40 to 30:1;

[0011] More preferably, the weight ratio of antibiotics of pyrimidine nucleosides to metalaxyl and its salts is 1:30 to 5:1;

[0012] The weight ratio of antibiotics of pyrimidine nucleosides to propamocarb and its salts is 1:35 to 5:1, the weight ratio of antibiotics of pyrimidine nucleosides to fluazinam is 1:15 to 10:1, and the weight ratio of antibiotics of pyrimidine nucleosides to activated ester is 1:5 to 20:1.

[0013] Another technical solution of the present invention is that the bactericidal composition containing antibiotics of pyrimidine nucleosides is used to control crop diseases, and the crops include fruit trees, vegetables, food crops, tea trees, and ornamental plants.

[0014] Furthermore, the diseases include black spot, phytophthora blight, powdery mildew, downy mildew, anthracnose, sheath blight, fusarium wilt, rust, phytophthora blight, late blight, black shank, damping-off, scab, leaf blotch, rice blast, gray mold.

[0015] The content of the active ingredient in the composition of the present invention depends on the application rate when used alone, also on the mixing ratio of one compound with another and the degree of synergistic effect, and is also related to the target disease. Generally, the weight percentage content of the active ingredient in the composition is 1% - 90% of the total weight, preferably 5% - 80%. According to different formulation types, the content range of the active ingredient varies. Generally, the liquid formulation contains 1% - 60% of the active substance by weight, preferably 5% - 50%; the solid formulation contains 5% - 80% of the active substance by weight, preferably 10% - 80%.

[0016] The bactericidal composition of the present invention contains at least one surfactant to facilitate the dispersion of the active component in water during application. The content of the surfactant is 2% - 30% of the total weight of the formulation, and the balance is a solid or liquid diluent.

[0017] The surfactants selected for the bactericidal composition of the present invention are well-known to those skilled in the art: it can be selected from one or several of dispersants, wetting agents, thickeners or defoamers. According to different dosage forms, the formulation can also contain stabilizers, antifreeze agents, etc. well-known to those skilled in the art.

[0018] The bactericidal composition of the present invention can be diluted by the user before use or used directly. Its preparation can be prepared by processing methods well-known to those skilled in the art, that is, after mixing the active ingredient with a liquid solvent or a solid carrier, then adding one or several of surfactants such as dispersants, stabilizers, wetting agents, binders, defoamers, etc.

[0019] The bactericidal composition of the present invention can be processed into any pesticide-acceptable dosage form as needed. The preferred dosage forms are wettable powders, water-dispersible granules, suspensions, aqueous solutions, soluble powders, microemulsions, emulsifiable concentrates, microcapsule suspensions, soluble liquid formulations, and can also be made into suspoemulsions, microcapsule suspension-suspensions.

[0020] When the composition is made into a wettable powder, it contains the following components and contents: active ingredient A 1% - 60%, active ingredient B 1% - 80%, dispersant 1% - 12%, wetting agent 1% - 8%, filler balance.

[0021] When the composition is made into a water-dispersible granule, it includes the following components and contents: active ingredient A 1% - 60%, active ingredient B 1% - 80%, dispersant 1% - 12%, wetting agent 1% - 8%, disintegrant 1% - 10%, binder 0 - 8%, filler balance.

[0022] When the composition is made into a suspending agent, it includes the following components and contents: active ingredient A 1% - 50%, active ingredient B 1% - 50%, dispersant 1% - 10%, wetting agent 1% - 10%, defoaming agent 0.01% - 2%, thickening agent 0.05% - 2%, antifreeze 0 - 8%, deionized water added to 100%.

[0023] When the composition is made into an aqueous solution, it includes the following components and contents: active ingredient A 0.5% - 40%, active ingredient B 0.5% - 40%, cosolvent 2% - 6%, wetting agent 1% - 10%, antifreeze 0 - 8%, deionized water added to 100%.

[0024] When the composition is made into a soluble powder, it includes the following component contents: active ingredient A 1% - 60%, active ingredient B 1% - 80%, dispersant 2% - 8%, wetting agent 1% - 7%, stabilizer 0 - 5%, filler the balance.

[0025] When the composition is made into a microemulsion, it includes the following components and contents: active ingredient A 0.5% - 50%, active ingredient B 0.5% - 50%, emulsifier 1% - 15%, solvent 1% - 10%, antifreeze 0 - 8%, stabilizer 0 - 3%, defoaming agent 0.01% - 2%, deionized water added to 100%.

[0026] When the composition is made into an emulsion in water, it includes the following components and contents: active ingredient A 0.5% - 50%, active ingredient B 0.5% - 50%, solvent 1% - 20%, emulsifier 1% - 12%, antifreeze 0 - 8%, defoaming agent 0.01% - 2%, thickening agent 0.05% - 2%, deionized water added to 100%.

[0027] When the composition is made into a microcapsule suspending agent, it includes the following components and contents: active ingredient A 0.5% - 50%, active ingredient B 0.5% - 50%, high molecular capsule wall material 1% - 10%, dispersant 2% - 10%, solvent 1% - 10%, emulsifier 1% - 7%, pH regulator 0.1% - 5%, defoaming agent 0.1% - 2%, deionized water added to 100%.

[0028] When the composition is made into a soluble liquid agent, it includes the following component contents: active ingredient A 1% - 60%, active ingredient B 1% - 80%, emulsifier 1% - 10%, cosolvent 2% - 6%, solvent added to 100%.

[0029] The main technical indicators of the wettable powder of the present invention:

[0030]

[0031] The main technical indicators of the water dispersible granules of the present invention:

[0032]

[0033] The main technical indicators of the suspending agent of the present invention:

[0034]

[0035] The main technical indicators of the aqueous agent of the present invention:

[0036]

[0037] The main technical indicators of the soluble powder of the present invention:

[0038]

[0039] The main technical indicators of the microemulsion of the present invention:

[0040]

[0041] The main technical indicators of the oil-in-water emulsion of the present invention:

[0042]

[0043] The main technical indicators of the microcapsule suspension of the present invention:

[0044]

[0045] The main technical indicators of the soluble liquid of the present invention:

[0046]

[0047] Based on the above technical solutions, the present invention has the following technical effects:

[0048] (1) The composition of the present invention has good synergistic and long-lasting effects within a certain range, and the control effect is higher than that of the single agent; (2) The dosage of pesticides is reduced, the residue of pesticides on crops is reduced, and environmental pollution is alleviated; (3) The bactericidal spectrum is expanded, and it has high activity against various diseases such as black spot, blight, powdery mildew, downy mildew, anthracnose, sheath blight, fusarium wilt, rust, phytophthora blight, late blight, black shank, damping-off, scab, apple blotch leaf spot, rice blast, and gray mold. Specific embodiments

[0049] The present invention will be further described below in conjunction with examples. The percentages in the examples are all weight percentages, but the present invention is not limited thereto.

[0050] Application Example 1

[0051] Examples 1 to 10 Wettable powder

[0052] Mix the pyrimidine nucleoside antibiotics, active ingredient B, dispersant, wetting agent, and filler, mix them evenly in a mixing tank, crush them with a jet mill, and then mix them evenly to obtain the wettable powder product of the present invention. See Tables 1 and 2 for details.

[0053] Table 1 Components and Contents of Examples 1-5

[0054]

[0055] Table 2 Components and Contents of Examples 6-10

[0056]

[0057] Interchange metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar-S-methyl in the table to obtain a new preparation.

[0058] Examples 11-18 Water Dispersible Granules

[0059] Mix the pyrimidine nucleoside antibiotics, active ingredient B, dispersant, wetting agent, disintegrant, and filler, and obtain the required particle size through jet milling to get the granulation material. Quantitatively feed the material into a fluidized bed granulator dryer, and after granulation and drying, obtain the water dispersible granule product of the present invention. See Tables 3 and 4 for details.

[0060] Table 3 Components and Contents of Examples 11-14

[0061]

[0062] Table 4 Components and Contents of Examples 15-18

[0063]

[0064] Interchange metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar-S-methyl in the table to obtain a new preparation.

[0065] Examples 19-24 Suspension Concentrates

[0066] Mix the dispersant, wetting agent, defoamer, thickener, and antifreeze agent evenly through high-speed shearing, add the pyrimidine nucleoside antibiotics and active ingredient B, and make up the balance with deionized water. Ball mill in a ball mill for 2-3 hours to make all the particle sizes of the fine particles below 5 μm, and obtain the suspension concentrate product of the present invention. See Table 5 for details.

[0067] Table 5 Components and Contents of Examples 19-24

[0068]

[0069] Interchange metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar-S-methyl in the table to obtain a new preparation.

[0070] Examples 25 - 31 Aqueous Solutions

[0071] Mix the pyrimidine nucleoside antibiotics, metalaxyl and its salts or propamocarb and its salts, cosolvent, wetting agent, antifreeze agent through high - speed shearing until homogeneous, and make up the balance with deionized water to obtain the aqueous solution product of the present invention. See Table 6 for details.

[0072] Table 6 Components and Contents of Examples 25 - 31

[0073]

[0074]

[0075] By interchanging metalaxyl and its salts, propamocarb and its salts in the table, new preparations can be obtained.

[0076] Examples 32 - 37 Soluble Powders

[0077] Mix the pyrimidine nucleoside antibiotics, metalaxyl and its salts or propamocarb and its salts, dispersant, wetting agent, stabilizer, filler, and mix them evenly in a mixing tank. After grinding with an air - stream pulverizer and then mixing evenly again, the soluble powder product of the present invention can be obtained. See Table 7 for details.

[0078] Table 7 Components and Contents of Examples 32 - 37

[0079]

[0080] By interchanging metalaxyl and its salts, propamocarb and its salts in the table, new preparations can be obtained.

[0081] Examples 38 - 43 Microemulsions

[0082] Dissolve the pyrimidine nucleoside antibiotics and active ingredient B in a homogenizer containing a solvent, add the emulsifier, antifreeze agent, defoamer to the homogenizer containing the above - mentioned solution, make up the balance with deionized water, then mix and homogenize strongly. Finally, the microemulsion product of the present invention with clear and transparent appearance can be obtained. See Table 8 for details.

[0083] Table 8 Components and Contents of Examples 38 - 43

[0084]

[0085] By interchanging metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar - S - methyl in the table, new preparations can be obtained.

[0086] Examples 44 - 47 Emulsifiable Concentrates

[0087] Mix the pyrimidine nucleoside antibiotics, active ingredient B, solvent, and emulsifier together to dissolve them into a homogeneous oil phase; mix deionized water, antifreeze, thickener, and defoamer together to form a homogeneous aqueous phase. Under high-speed stirring, add the aqueous phase to the oil phase to obtain the aqueous emulsion product of the present invention. See Table 9 for details.

[0088] Table 9 Components and Contents of Examples 44 - 47

[0089]

[0090]

[0091] By interchanging metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar - S - methyl in the table, new preparations can be obtained.

[0092] Microcapsule Suspensions of Examples 48 - 51

[0093] Mix the pyrimidine nucleoside antibiotics, active ingredient B, high - molecular capsule wall material, and solvent together to dissolve them into a homogeneous oil phase. Under shear conditions, add the oil phase to the aqueous solution containing emulsifier, pH regulator, dispersant, and defoamer, and make up the balance with deionized water. The two materials react at the oil - water interface to form a high - molecular capsule wall, obtaining a microcapsule suspension product with good dispersion of the composition of the present invention. See Table 10 for details.

[0094] Table 10 Components and Contents of Examples 48 - 51

[0095]

[0096] By interchanging metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar - S - methyl in the table, new preparations can be obtained.

[0097] Soluble Liquid Agents of Examples 52 - 58

[0098] Dissolve the pyrimidine nucleoside antibiotics, metalaxyl and its salts or propamocarb and its salts in a homogenizer equipped with a cosolvent. Add the emulsifier and solvent to the homogenizer containing the above solution, make up the balance with solvent, and then mix and homogenize strongly. Finally, obtain the clear and transparent soluble liquid agent product of the present invention. See Table 11 for details.

[0099] Table 11 Components and Contents of Examples 52 - 58

[0100]

[0101] By interchanging metalaxyl and its salts, propamocarb and its salts in the table, new preparations can be obtained.

[0102] The embodiments of the present invention adopt a method combining indoor toxicity determination and field trials. First, through indoor toxicity determination, the synergistic ratio (SR) after the two agents are compounded in a certain proportion is determined. When SR < 0.5, it is an antagonistic effect; when 0.5 ≤ SR ≤ 1.5, it is an additive effect; when SR > 1.5, it is a synergistic effect. On this basis, field trials are then carried out.

[0103] Test method: After determining the effective inhibitory concentration range of each agent through preliminary tests, 5 dosage treatments are set for each agent according to the active ingredient content, and a clear water control is set. Referring to the "Pesticide Indoor Bioassay Test Guidelines Fungicides", the mycelial growth rate method is used to determine the toxicity of the agents to crop pathogens. After 72 hours, the colony diameter is measured by the cross method, and the net growth amount and mycelial growth inhibition rate of each treatment are calculated.

[0104] Net growth amount (mm) = measured colony diameter - 5

[0105]

[0106] The mycelial growth inhibition rate is converted into probit value (y), and the liquid medicine concentration (μg / mL) is converted into logarithm value (x). The toxicity regression equation (y = a + bx) is obtained by the least square method, and the EC50 value of each agent is calculated therefrom. At the same time, the combined synergistic ratio (SR) of different ratios of the two agents is calculated according to the Wadley method. When SR < 0.5, it is an antagonistic effect; when 0.5 ≤ SR ≤ 1.5, it is an additive effect; when SR > 1.5, it is a synergistic effect. The calculation formula is as follows:

[0107]

[0108] Where: a and b are the proportions of active ingredient A and active ingredient B in the combination respectively;

[0109] A is a pyrimidine nucleoside antibiotic;

[0110] B is selected from one of metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar - S - methyl.

[0111] Application Example 2

[0112] Tested disease: Grape downy mildew.

[0113] All the tested agents are provided by Shaanxi Welch Crop Protection Co., Ltd.

[0114] Test design: After preliminary tests, the effective inhibitory concentration ranges of agro - antibiotic 120, metalaxyl and its salts technical, and their mixed agents with different ratios are determined.

[0115] Toxicity determination results

[0116] Table 12 Analysis table of the toxicity determination results of the compounding of agro - antibiotic 120 and metalaxyl and its salts against grape downy mildew

[0117]

[0118] As can be seen from Table 12, when the ratio of Nongkang 120 to metalaxyl and its salts is in the range of 1:80 to 60:1, the synergistic ratio SR for grape downy mildew is greater than 1.5, indicating that the two show a synergistic effect when mixed within the range of 1:80 to 60:1. When the ratio of Nongkang 120 to metalaxyl and its salts is in the range of 1:30 to 5:1, the synergistic effect is more prominent, and the synergistic ratios are all above 2.20. Especially when the weight ratio of Nongkang 120 to metalaxyl and its salts is 1:6, the synergistic ratio is the largest and the synergistic effect is the most obvious. Through the applicant's experiments, it is found that when the ratios of Nongkang 120 to metalaxyl and its salts are 5:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:20, 1:25, 1:30, the compound of Nongkang 120 with metalaxyl and its salts has obvious synergistic effects on the control of Alternaria mali, Phytophthora infestans, Erysiphe cichoracearum, Plasmopara viticola, Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum, Puccinia, Phytophthora capsici, Phytophthora infestans, Pseudomonas solanacearum, Pythium aphanidermatum, Venturia inaequalis, Alternaria alternata apple pathotype, Magnaporthe oryzae, Botrytis cinerea, and the synergistic ratios are all above 1.50.

[0119] Application Example Three

[0120] Tested disease: Tomato late blight.

[0121] All the test agents were provided by Shaanxi Welch Crop Protection Co., Ltd.

[0122] Test design: Through preliminary tests, the effective inhibitory concentration range of Nongkang 120, propamocarb and its salt technicals and their mixed agents with different ratios was determined.

[0123] Toxicity determination results

[0124] Table 13 Analysis table of the toxicity determination results of the compound of Nongkang 120 with propamocarb and its salt against tomato late blight

[0125]

[0126] As can be seen from Table 13, when the ratio of Agricamycin 120 to propamocarb and its salt is between 1:80 and 60:1, the synergistic ratio SR for the control of late blight of tomato is greater than 1.5, indicating that the mixture of the two shows a synergistic effect within the range of 1:80 to 60:1. When the ratio of Agricamycin 120 to propamocarb and its salt is between 1:35 and 5:1, the synergistic effect is more obvious and prominent, and the synergistic ratio is above 2.20. Especially when the weight ratio of Agricamycin 120 to propamocarb and its salt is 1:7, the synergistic ratio is the largest and the synergistic effect is the most obvious. Through the applicant's experiments, it is found that when the ratio of Agricamycin 120 to propamocarb and its salt is 5:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:20, 1:25, 1:30, 1:35, the compound of Agricamycin 120 and propamocarb and its salt has obvious synergistic effects on the control of Alternaria leaf spot, Phytophthora blight, Powdery mildew, Downy mildew, Anthracnose, Sheath blight, Fusarium wilt, Rust, Phytophthora blight, Late blight, Black shank, Damping-off, Scab, Alternaria blotch, Rice blast, Gray mold, and the synergistic ratio is above 1.50.

[0127] Application Example Four

[0128] Test disease: Alternaria blotch of apple.

[0129] All test agents were provided by Shaanxi Welch Crop Protection Co., Ltd.

[0130] Test design: Through preliminary tests, determine the effective inhibitory concentration range of the mixture of Agricamycin 120 and fluazinam technical and their different ratios.

[0131] Results of toxicity determination

[0132] Table 14 Analysis table of the results of the toxicity determination of the compound of Agricamycin 120 and fluazinam against Alternaria blotch of apple

[0133]

[0134]

[0135] As can be seen from Table 14, when the ratio of Nongkang 120 to fluazinam is between 1:80 and 60:1, the synergistic ratio SR for apple marssonina leaf blotch is greater than 1.5, indicating that the two show a synergistic effect when mixed within the range of 1:80 to 60:1. When the ratio of Nongkang 120 to fluazinam is between 1:15 and 10:1, the synergistic effect is more obvious, and the synergistic ratio is above 2.20. In particular, when the weight ratio of Nongkang 120 to fluazinam is 2:3, the synergistic ratio is the largest and the synergistic effect is the most obvious. Through the applicant's experiments, it is found that when the ratio of Nongkang 120 to fluazinam is 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 2:3, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:15, the compounding of Nongkang 120 and fluazinam has an obvious synergistic effect on the control of black spot, phytophthora blight, powdery mildew, downy mildew, anthracnose, sheath blight, fusarium wilt, rust, phytophthora blight, late blight, black shank, damping-off, scab, marssonina leaf blotch, rice blast, gray mold, and the synergistic ratio is above 1.50.

[0136] Application Example Five:

[0137] Test disease: Wheat rust.

[0138] All test agents were provided by Shaanxi Welch Crop Protection Co., Ltd.

[0139] Test design: Through preliminary tests, determine the effective inhibitory concentration range of Nongkang 120, activator ester technical, and their mixed agents with different ratios.

[0140] Virulence determination results

[0141] Table 15 Analysis table of virulence determination results of the compounding of Nongkang 120 and activator ester against wheat rust

[0142]

[0143] As can be seen from Table 15, when the ratio of agricultural antibiotic 120 to activin is in the range of 1:80 to 60:1, the synergistic ratio SR for wheat rust is greater than 1.5, indicating that the mixture of the two shows a synergistic effect within the range of 1:80 to 60:1. When the ratio of agricultural antibiotic 120 to activin is in the range of 1:5 to 20:1, the synergistic effect is more obvious, and the synergistic ratio is above 2.10. Especially when the weight ratio of agricultural antibiotic 120 to activin is 4:1, the synergistic ratio is the largest and the synergistic effect is the most obvious. Through the applicant's experiments, it is found that when the ratio of agricultural antibiotic 120 to activin is 20:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, the compound of agricultural antibiotic 120 and activin has obvious synergistic effects on the control of alternaria leaf spot, phytophthora blight, powdery mildew, downy mildew, anthracnose, sheath blight, fusarium wilt, rust, phytophthora blight, late blight, black shank, damping-off, scab, apple blotch leaf spot, rice blast, gray mold, and the synergistic ratio is above 1.50.

[0144] Application Example Six

[0145] Pharmacodynamic Experiment on the Control of Pear Scab by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0146] This experiment was arranged in Xianyang City, Shaanxi Province. The test agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 4% pyrimidine nucleoside antibiotic aqueous solution (purchased on the market), 25% metalaxyl wettable powder (purchased on the market), 35% propamocarb aqueous solution (purchased on the market), 500 g / L fluazinam suspension (purchased on the market), and 24% activin suspension (self-prepared).

[0147] The disease condition of pear scab was investigated before applying the medicine. The first application was carried out at the initial stage of the disease condition, and the medicine was applied once every 7 days for a total of 2 times. The disease index was investigated 7 days, 14 days, and 30 days after the second application, and the control effect was calculated. The experimental results are as follows:

[0148] Table 16 Pharmacodynamic Experiment on the Control of Pear Scab by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0149]

[0150] As can be seen from Table 16, the compound of pyrimidine nucleoside antibiotics and active ingredient B can effectively control pear scab, and the control effect is better than that of single agents, and the control effect period is long. There is no adverse effect on the target crops within the test dosage range.

[0151] Application Example Seven

[0152] Pharmacodynamic Experiment on the Control of Wheat Rust by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0153] This experiment was arranged in Taierzhuang, Zaozhuang City, Shandong Province. The experimental pesticides were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents included 4% pyrimidine nucleoside antibiotic aqueous solution (commercially available), 25% metalaxyl wettable powder (commercially available), 35% propamocarb aqueous solution (commercially available), 500g / L fluazifop suspension (commercially available), and 24% activated ester suspension (self-prepared).

[0154] Before applying the medicine, investigate the wheat rust disease. Apply the medicine for the first time at the early stage of the disease, and apply the medicine once every 7 days, for a total of 2 times. 7 days, 14 days, and 30 days after the second application, investigate the disease index and calculate the prevention effect. The experimental results are as follows:

[0155] Table 17 Pyrimidine nucleoside antibiotics and active ingredient B and their combination for controlling wheat rust

[0156]

[0157] It can be seen from Table 17 that the combination of pyrimidine nucleoside antibiotics and active ingredient B can effectively control wheat rust, and the control effect is better than that of a single agent, and the control period is long. There is no adverse effect on the target crops within the test range.

[0158] Application Example 8

[0159] Experimental study on the efficacy of pyrimidine nucleoside antibiotics, active ingredient B and their combination in controlling apple leaf spot disease

[0160] This experiment was arranged in Xianyang City, Shaanxi Province. The experimental agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd., and the control agents included 4% pyrimidine nucleoside antibiotic aqueous solution (commercially available), 25% metalaxyl wettable powder (commercially available), 35% propanedionyl aqueous solution (commercially available), 500g / L fluazifop suspension (commercially available), and 24% activated ester suspension (self-prepared).

[0161] Before applying the medicine, the condition of apple leaf spot disease was investigated. The first application was made at the early stage of the disease, and the medicine was applied once every 7 days, for a total of 3 applications. The disease index was investigated 7 days, 14 days, and 30 days after the third application, and the control effect was calculated. The experimental results are shown below:

[0162] Table 18 Pyrimidine nucleoside antibiotics and active ingredient B and their combination for the prevention and treatment of apple leaf spot disease

[0163]

[0164] It can be seen from Table 18 that the combination of pyrimidine nucleoside antibiotics and active ingredient B can effectively control apple leaf spot disease, and the control effect is better than that of a single agent, and the control period is long. There is no adverse effect on the target crops within the test range.

[0165] Application Example 9

[0166] Pharmacodynamic Experiment on the Control of Sheath Blight of Rice by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0167] This experiment was arranged in Hanzhong City, Shaanxi Province. The experimental agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 4% pyrimidine nucleoside antibiotic aqueous solution (purchased on the market), 25% metalaxyl wettable powder (purchased on the market), 35% propamocarb aqueous solution (purchased on the market), 500 g / L fluazinam suspension concentrate (purchased on the market), and 24% acibenzolar-s-methyl suspension concentrate (self-prepared).

[0168] The sheath blight of rice was investigated before application. The first application was carried out at the initial stage of the disease, and the application was carried out once every 7 days for a total of 2 times. The disease index was investigated and the control effect was calculated 7 days, 14 days, and 30 days after the second application. The experimental results are as follows:

[0169] Table 19 Pharmacodynamic Experiment on the Control of Sheath Blight of Rice by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0170]

[0171] It can be seen from Table 19 that the mixture of pyrimidine nucleoside antibiotics and active ingredient B can effectively control sheath blight of rice. The control effects are better than those of single agents, and the control effect period is long. There is no adverse effect on the target crops within the scope of experimental application.

[0172] Application Example Ten

[0173] Pharmacodynamic Experiment on the Control of Downy Mildew of Cucumber by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0174] This experiment was arranged in Jingyang County, Shaanxi Province. The experimental agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 4% pyrimidine nucleoside antibiotic aqueous solution (purchased on the market), 25% metalaxyl wettable powder (purchased on the market), 35% propamocarb aqueous solution (purchased on the market), 500 g / L fluazinam suspension concentrate (purchased on the market), and 24% acibenzolar-s-methyl suspension concentrate (self-prepared).

[0175] The downy mildew of cucumber was investigated before application. The first application was carried out at the initial stage of the disease, and the application was carried out once every 7 days for a total of 2 times. The disease index was investigated and the control effect was calculated 7 days, 14 days, and 30 days after the second application. The experimental results are as follows:

[0176] Table 20 Pharmacodynamic Experiment on the Control of Downy Mildew of Cucumber by Pyrimidine Nucleoside Antibiotics, Active Ingredient B and Their Mixtures

[0177]

[0178] As can be seen from Table 20, the compounding of pyrimidine nucleoside antibiotics and active ingredient B can effectively control cucumber downy mildew. The control effects are all better than those of single agents, and the control effect period is long. There is no adverse effect on the target crops within the test dosage range.

[0179] Application Example XI

[0180] Pharmacodynamic experiment on the control of Botrytis cinerea of grapes by pyrimidine nucleoside antibiotics, active ingredient B and their compound

[0181] This experiment was arranged in the suburbs of Weinan City, Shaanxi Province. The test agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 4% pyrimidine nucleoside antibiotic aqueous solution (purchased on the market), 25% metalaxyl wettable powder (purchased on the market), 35% propamocarb aqueous solution (purchased on the market), 500g / L fluazinam suspension (purchased on the market), 24% acibenzolar-s-methyl suspension (self-prepared).

[0182] The disease situation of Botrytis cinerea of grapes was investigated before application. The first application was carried out at the initial stage of the disease, and the application was carried out once every 7 days for a total of 2 times. The disease index was investigated and the control effect was calculated 7 days, 14 days and 30 days after the second application. The experimental results are as follows:

[0183] Table 21 Pharmacodynamic experiment on the control of Botrytis cinerea of grapes by pyrimidine nucleoside antibiotics, active ingredient B and their compound

[0184]

[0185] As can be seen from Table 21, the compounding of pyrimidine nucleoside antibiotics and active ingredient B can effectively control Botrytis cinerea of grapes. The control effects are all better than those of single agents, and the control effect period is long. There is no adverse effect on the target crops within the test dosage range.

[0186] Application Example XII

[0187] Pharmacodynamic experiment on the control of tomato blight by pyrimidine nucleoside antibiotics, active ingredient B and their compound

[0188] This experiment was arranged in the suburbs of Xi'an City, Shaanxi Province. The test agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 4% pyrimidine nucleoside antibiotic aqueous solution (purchased on the market), 25% metalaxyl wettable powder (purchased on the market), 35% propamocarb aqueous solution (purchased on the market), 500g / L fluazinam suspension (purchased on the market), 24% acibenzolar-s-methyl suspension (self-prepared).

[0189] The disease situation of tomato blight was investigated before application. The first application was carried out at the initial stage of the disease, and the application was carried out once every 7 days for a total of 2 times. The disease index was investigated and the control effect was calculated 7 days, 14 days and 30 days after the second application. The experimental results are as follows:

[0190] Table 22 Pharmacodynamic experiment on the control of tomato blight by pyrimidine nucleoside antibiotics, active ingredient B and their compound

[0191]

[0192] As can be seen from Table 22, the pyrimidine nucleoside antibiotics can effectively prevent and control tomato blight after being compounded with active ingredient B. The control effects are better than those of single agents, and the control effect period is long. There is no adverse effect on the target crops within the test dosage range.

[0193] Application Example XIII

[0194] Pharmacodynamic experiment on the prevention and control of rose powdery mildew by pyrimidine nucleoside antibiotics and active ingredient B and their compound

[0195] This experiment was arranged in Pingyin County, Jinan City, Shandong Province. The test agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 4% pyrimidine nucleoside antibiotic aqueous solution (purchased on the market), 25% metalaxyl wettable powder (purchased on the market), 35% propamocarb aqueous solution (purchased on the market), 500 g / L fluazinam suspension (purchased on the market), and 24% acibenzolar-S-methyl suspension (self-prepared).

[0196] The disease condition of rose powdery mildew was investigated before applying the medicine. The first application was made at the initial stage of the disease condition, and the medicine was applied once every 7 days for a total of 2 times. The disease index was investigated and the control effect was calculated 7 days, 14 days, and 30 days after the second application. The experimental results are as follows:

[0197] Table 23 Pharmacodynamic experiment on the prevention and control of rose powdery mildew by pyrimidine nucleoside antibiotics and active ingredient B and their compound

[0198]

[0199] As can be seen from Table 23, the pyrimidine nucleoside antibiotics can effectively prevent and control rose powdery mildew after being compounded with active ingredient B. The control effects are better than those of single agents, and the control effect period is long. There is no adverse effect on the target crops within the test dosage range.

[0200] After experiments in different places across the country, it was found that the compound of pyrimidine nucleoside antibiotics with metalaxyl and its salts, propamocarb and its salts, fluazinam, and acibenzolar-S-methyl has a control effect of over 95% on common diseases such as black spot, blight, powdery mildew, downy mildew, anthracnose, sheath blight, fusarium wilt, rust, phytophthora blight, late blight, black shank, damping-off, scab, target leaf spot, rice blast, and gray mold on various crops, which is better than the control effect of single agents and has an obvious synergistic effect.

[0201] Obviously, the above-mentioned embodiments of the present invention are merely examples for clearly illustrating the present invention, rather than limiting the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all implementation manners here. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A bactericidal composition, characterized in that: The effective active ingredients include active ingredient A and active ingredient B, and the weight ratio of active ingredient A to active ingredient B is 1:80 to 60:

1. The active ingredient A is a pyrimidine nucleoside antibiotic, and the active ingredient B is metalaxyl and its salts.

2. The bactericidal composition containing pyrimidine nucleoside antibiotics according to claim 1, characterized in that: The weight ratio of active ingredient A to active ingredient B is 1:40 to 30:

1.

3. The bactericidal composition containing pyrimidine nucleoside antibiotics according to claim 2, characterized in that: The weight ratio of the pyrimidine nucleoside antibiotic to metalaxyl and its salts is 1:30 to 5:1; The composition is made into wettable powder, water dispersible granule, suspension concentrate, aqueous solution, soluble powder, microemulsion, emulsion in water, microcapsule suspension, soluble liquid formulation.

4. Use of the bactericidal composition containing pyrimidine nucleoside antibiotics as claimed in claim 4 in controlling crop diseases.

5. The application according to claim 5, wherein: The diseases include black spot, phytophthora blight, powdery mildew, downy mildew, anthracnose, sheath blight, fusarium wilt, rust, phytophthora rot, late blight, black shank, damping-off, scab, apple blotch leaf spot, rice blast, grey mold.

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