Multifunctional hand-care hydrogel based on chitosan / hyaluronic acid skeleton network and preparation method of multifunctional hand-care hydrogel

Abstract

The invention discloses multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network and a preparation method of the multifunctional hand care hydrogel. The multifunctional hand care hydrogel is prepared from a chitosan / hyaluronic acid skeleton network material and a multifunctional solution, the multifunctional solution is prepared from the following raw materials in parts by weight: 0.5 to 1 part of sodium hyaluronate, 0.001 to 0.005 part of essential oil, 0.05 to 0.5 part of xanthan gum, 0.1 to 0.5 part of gelatin, 0.0005 to 0.002 part of benzalkonium chloride, 0.05 to 0.2 part of salidroside and 100 to 150 parts of water; the multifunctional hand-care hydrogel provided by the invention can quickly form a film, and has the functions of inhibiting bacteria, preserving moisture and resisting oxidation; the preparation method is mild in preparation condition, low in cost, low in equipment requirement, green and pollution-free, and has huge development potential.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and particularly relates to a multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network and a preparation method thereof. Background Art

[0002] With the increasing requirements for hand antibacterial and protection, in recent years, there have been more and more hand skin care or antibacterial products, such as hand creams, hand sanitizers, disinfectants, hand gels, etc.

[0003] Hand creams mainly focus on the function of moisturizing, with a single function; hand sanitizers need to be washed with water after use, which is inconvenient to use; disinfectants mainly focus on the function of antibacterial, with a single function; hand gels have a refreshing gel texture, showing good biocompatibility, hydrophilicity, skin-friendliness, moisturizing property, antioxidant property and antibacterial property, etc. The comprehensive performance is significantly higher than that of traditional hand creams, and has gradually become a new choice for people's hand care.

[0004] Chinese Patent CN118415951A discloses a hand gel and a preparation method thereof. According to mass percentage, it at least includes the following raw materials: Component A: 0.1-3% thickener, 1-5% moisturizer A, water; the moisturizer includes glycerin and panthenol; Component B: 3-8% emollient, 0.5-3% antioxidant, 0.01-0.1% Salicornia herbacea extract; Component C: 0.5-3% hyaluronic acid mixture; Component D: 1-5% moisturizer B; Component E: 0.005-0.02% Lithothamnion calcareum extract; Component F: 0-1% preservative; Component G: 0.01-0.1% Vanilla planifolia fruit extract. However, the components of the hand gel in this patent are complex, containing easily sensitizing components such as synthetic preservatives and fragrances, with a high cost, and lack of safety. Moreover, it only has the functions of moisturizing and anti-wrinkle, and the functions are relatively single. Summary of the Invention

[0005] To solve the above technical problems, the present invention provides a multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network and a preparation method thereof, which can quickly form a film, and has both antibacterial, moisturizing and antioxidant functions. Its preparation conditions are mild, the cost is low, the equipment requirements are not high, and it is green and pollution-free, having great development potential.

[0006] The technical solution adopted by the present invention is as follows:

[0007] A multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network, wherein the multifunctional hand care hydrogel is composed of a chitosan / hyaluronic acid skeleton network material and a multifunctional solution;

[0008] The multifunctional solution comprises the following raw materials in parts by weight: 0.5 - 1 part of sodium hyaluronate, 0.001 - 0.005 part of essential oil, 0.05 - 0.5 part of xanthan gum, 0.1 - 0.5 part of gelatin, 0.0005 - 0.002 part of benzalkonium chloride, 0.05 - 0.2 part of salidroside, and 100 - 150 parts of water.

[0009] Furthermore, the mass ratio of the chitosan / hyaluronic acid skeleton network material to the multifunctional solution is 1:10 - 50.

[0010] The preparation method of the chitosan / hyaluronic acid skeleton network material comprises the following steps:

[0011] (1) Heat and stir sodium hyaluronate powder in water until it is completely dissolved, and then add chitosan powder and stir to make it evenly dispersed;

[0012] (2) Dropwise add glacial acetic acid solution to step (1) until the chitosan is completely dissolved, and continue to stir until it is evenly mixed;

[0013] (3) Let the reaction solution obtained in step (2) stand at room temperature for 2 - 24 h;

[0014] (4) Separate and obtain the precipitate in step (3);

[0015] (5) Add deionized water to the precipitate obtained in step (4), stir to make it evenly dispersed, stand at room temperature for 2 - 24 h, and obtain a precipitate by layering;

[0016] (6) Repeat the washing process of step (5) twice for the precipitate obtained in step (5), separate the precipitate, and obtain the chitosan / hyaluronic acid skeleton network material.

[0017] Furthermore, in step (1), the mass ratio of sodium hyaluronate to chitosan is 1 - 3:2; the mass concentration of sodium hyaluronate in water is 0.05 - 2%.

[0018] In step (2), the mass concentration of glacial acetic acid is 0.5 - 5%; when dropping the glacial acetic acid solution, the temperature of the system is 40 - 50 °C. The volume of the added glacial acetic acid solution is 20 - 50% of the volume of water; the continuous stirring time is 5 - 10 min.

[0019] In step (5), the mass ratio of water to the precipitate is 1 - 10:1.

[0020] The chitosan / hyaluronic acid skeleton network material prepared by the above method has excellent tensile properties (190% - 350%), antibacterial properties (50% - 70%), and adjustable structural properties, etc.

[0021] The preparation method of the multifunctional solution is as follows: Heat and stir sodium hyaluronate in water until it is completely dissolved, then add essential oil and stir evenly; add xanthan gum and stir until it is completely dissolved; add gelatin solution and stir evenly; finally, add benzalkonium chloride solution and salidroside solution and stir evenly to form the multifunctional solution.

[0022] The temperature of the heat and stir is 45-55 °C.

[0023] The present invention also provides a preparation method of the multifunctional hand-care hydrogel based on the chitosan / hyaluronic acid skeleton network. The preparation method includes the following steps: Disperse the chitosan / hyaluronic acid skeleton network material into the multifunctional solution, stir and mix at 35-45 °C for at least 30 min, perform ultrasonic treatment for 20-60 min, stand still at 60 °C for 30 min respectively, then place it at 4 °C and stand still for 90 min, and alternate and circulate 3 times.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The multifunctional hand-care hydrogel based on the chitosan / hyaluronic acid skeleton network provided by the present invention uses the chitosan / sodium hyaluronate skeleton network material as the mechanical property support, endowing the product with fast film-forming property; uses the multifunctional solution as the performance support, endowing the product with excellent antibacterial property, antioxidant property, moisturizing property, etc. Salidroside in its raw materials can not only increase the antioxidant property of the functional solution, but also contains numerous hydroxyl groups, which can be used as a physical cross-linking agent to form a hydrogen bond network with biological macromolecules such as hyaluronic acid in the functional solution, greatly improving the viscosity of the functional solution.

[0026] In the preparation method of the multifunctional hand-care hydrogel based on the chitosan / hyaluronic acid skeleton network provided by the present invention, during the process that the macromolecules contained in the functional solution penetrate into the chitosan / hyaluronic acid skeleton network through ultrasonic treatment and alternate hot and cold treatment, rich chain entanglements are formed, further improving the viscosity of the hand-care hydrogel and enhancing its film-forming property. The formed hydrogel blocks the direct contact between the skin and the outside world, improving the moisturizing property of the skin. The preparation method of the multifunctional hand-care hydrogel based on the chitosan / hyaluronic acid skeleton network provided by the present invention has mild preparation conditions, low cost, low equipment requirements, and is green and pollution-free, having great development potential. Description of the Drawings

[0027] Figure 1 It is the test result of the antibacterial experiment;

[0028] Figure 2 It is the test result of the film-forming property experiment;

[0029] Figure 3 It is the test result of the moisturizing property experiment;

[0030] Figure 4 This is the test result of water solubility;

[0031] Figure 5 This is the test result of rheological properties;

[0032] Figure 6 This is the test result of antioxidant property. Specific implementation manners

[0033] The present invention will be described in detail below in conjunction with embodiments.

[0034] Sodium hyaluronate powder: with a purity of 95%, and the manufacturer is Xinjiang Fufeng Biotechnology Co., Ltd.;

[0035] Preparation of gelatin solution: Dissolve gelatin in distilled water and heat it in a water bath at 50 °C for 3 h until it is completely dissolved.

[0036] Unless otherwise specified, other raw materials are commercially available.

[0037] Embodiment 1

[0038] A multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network, which is composed of a chitosan / hyaluronic acid skeleton network material and a multifunctional solution in a mass ratio of 1:10, and the total system is 150 m.

[0039] The multifunctional solution includes the following raw materials in parts by weight: 1 part of sodium hyaluronate, 0.0025 part of essential oil, 0.2 part of xanthan gum, 0.3 part of gelatin, 0.0006 part of benzalkonium chloride, 0.15 part of salidroside, and 131 parts of water.

[0040] The preparation method of the chitosan / hyaluronic acid skeleton network material includes the following steps:

[0041] (1) Place 1.12 g of sodium hyaluronate powder, a natural biopolymer with an average molecular weight of 370,000 Da, in an enzyme-catalyzed parallel reactor containing 105 mL of deionized water, set the temperature to 45 °C, and the rotation speed to 500 rpm for dissolution. After complete dissolution, add 1.68 g of chitosan powder with an average molecular weight of 700,000 Da, and stir for 5 min to uniformly disperse the chitosan;

[0042] (2) Add 35 mL of 4% acetic acid solution by mass to step (1), and stir for 10 min. The resulting solution is defined as the precursor solution of chitosan / sodium hyaluronate hydrogel;

[0043] (3) Let the reaction solution obtained in step (2) stand at room temperature for 24 h;

[0044] (4) Separate the suspension and the precipitate in step (3), and retain the precipitate;

[0045] Take 30 mL of the precipitate obtained in step (4), add 300 mL of deionized water thereto, place it on a thermostatic heating magnetic stirrer, stir at 45 °C and 500 rpm for 10 min to make it evenly dispersed, let it stand at room temperature for 24 h, and obtain a precipitate by layering.

[0046] (6) Repeat the washing of the precipitate obtained in step (5) twice according to step (5), separate the precipitate, and obtain the chitosan / hyaluronic acid skeleton network material.

[0047] The preparation method of the multifunctional solution comprises the following steps: Dissolve 1.0 g of sodium hyaluronate powder, a natural biopolymer with an average molecular weight of 370,000 Da, in 100 mL of water by stirring in a water bath at 50 °C for 90 min until it is completely dissolved, then add 0.25 mL of lavender essential oil with a concentration of 1%, and stir evenly; add 0.2 g of xanthan gum and stir until it is completely dissolved; add 30 mL of a gelatin solution with a mass concentration of 1%, and stir for 30 min; finally, add 0.1 mL of a benzalkonium chloride solution with a mass concentration of 0.6% and 1 mL of a salidroside solution with a mass concentration of 15%, and stir for 50 min to form a multifunctional solution.

[0048] The preparation method of the multifunctional hand care hydrogel based on the chitosan / hyaluronic acid skeleton network is as follows: Disperse the chitosan / hyaluronic acid skeleton network material into the multifunctional solution, stir and mix at 40 °C for 30 min, sonicate for 40 min, let it stand at 60 °C for 30 min respectively, and then let it stand at 4 °C for 90 min, and alternate and cycle 3 times.

[0049] Example 2

[0050] A multifunctional hand care hydrogel based on the chitosan / hyaluronic acid skeleton network is composed of the chitosan / hyaluronic acid skeleton network material and the multifunctional solution according to a mass ratio of 1:50;

[0051] The preparation methods of the chitosan / hyaluronic acid skeleton network material, the multifunctional solution, and the multifunctional hand care hydrogel based on the chitosan / hyaluronic acid skeleton network are the same as those in Example 1.

[0052] Example 3

[0053] A multifunctional hand care hydrogel based on the chitosan / hyaluronic acid skeleton network is composed of the chitosan / hyaluronic acid skeleton network material and the multifunctional solution according to a mass ratio of 1:5;

[0054] The preparation methods of the chitosan / hyaluronic acid skeleton network material, the multifunctional solution, and the multifunctional hand care hydrogel based on the chitosan / hyaluronic acid skeleton network are the same as those in Example 1.

[0055] Comparative Example 1

[0056] Other conditions are the same as in Example 1, except that the multifunctional solution is replaced with a sodium hyaluronate solution with a mass concentration of 1%.

[0057] Comparative Example 2

[0058] Other conditions are the same as in Example 1, except that on the basis of Comparative Example 1, an equal amount of salidroside as in Example 1 is added.

[0059] Comparative Example 3

[0060] Other conditions are the same as in Example 1, except that the multifunctional solution is replaced with deionized water.

[0061] Test Example

[0062] Test the antibacterial performance, film-forming property, moisture retention property, solubility and viscosity of the hand care hydrogels in the above-mentioned examples and comparative examples.

[0063] I. Antibacterial Performance Detection

[0064] Use LB liquid medium to culture Escherichia coli and Staphylococcus aureus to the logarithmic growth phase respectively. After incubating 0.1 g of the sample with 3 mL of the bacterial liquid in the logarithmic growth phase in an incubator at 37 °C for 3 h, dilute 0.1 mL of the co-incubated bacterial liquid with physiological saline to 10 -6 , and use 0.1 mL of the co-incubated culture solution to perform coating operation on LB solid medium and culture at 37 °C for 16 h, and finally compare the number of colonies.

[0065] The measurement results are as Figure 1 shown. The antibacterial rates of the hand care hydrogels in Example 1, Example 2, and Example 3 are the highest, reaching 100%. The antibacterial rates of Comparative Example 1 against Escherichia coli and Staphylococcus aureus are 49% and 57% respectively, and the antibacterial performance is the worst; the antibacterial performance of Comparative Example 2 and Comparative Example 3 is in the middle. Among them, the antibacterial performance of Comparative Example 2 is higher than that of Comparative Example 3, indicating that both the network skeleton and salidroside have certain antibacterial effects.

[0066] II. Film-Forming Property Detection

[0067] Place strips of the same size on human skin, then take 0.2 g of the hand care hydrogel sample in Example 1 and apply it on the strip and the surface of human skin, dry it in natural conditions, slowly uncover it from the free end of the strip, observe the pulling situation of the strip on human skin, and the size of the film area at the edge of the strip, and qualitatively analyze the relative size of the film-forming property of the above samples. Test Example 1, Example 2, Comparative Example 1, and Comparative Example 2 respectively according to the same method.

[0068] The measurement results are as Figure 2As shown, the hand care hydrogels in Example 1 and Example 3 have obvious film-forming properties. While the film-forming properties of Example 2 and Comparative Example 3 are the worst. This indicates that the chitosan / hyaluronic acid network skeleton alone has weak film-forming properties, and its synergistic effect with the functional solution significantly improves the film-forming properties of the hydrogel, further demonstrating that during the penetration process of the network skeleton and the functional solution, a macromolecular entanglement domain is formed, promoting film formation. The differences in film-forming properties among Comparative Example 2, Comparative Example 1, and Comparative Example 3 also prove the entanglement effect of macromolecules in the functional solution. However, when the proportion of the functional solution increases significantly and the number of network skeletons decreases (such as in Example 2), its film-forming properties also decrease significantly, indicating that in the skeleton network and functional solution system, maintaining a certain skeleton proportion is crucial for maintaining film-forming properties.

[0069] III. Moisture Retention Detection

[0070] Cut fresh pigskin into pieces of 2 cm × 2 cm. Evenly apply 0.2 g of the test sample on the pigskin, weigh and record it as M0. Place the pigskin in a constant temperature and humidity box at 37 °C, take it out every 2 minutes to measure the total weight, and record it as M t . Weigh it and record it as M when the hydrogel is completely dry s . Then calculate the water retention rate according to formula (1).

[0071]

[0072] The measurement results are as Figure 3 shown. The order of water retention from high to low is: Example 1, Example 3, Comparative Example 2, Comparative Example 1, Example 2, Comparative Example 3. This is almost consistent with the changing trend of film-forming properties. It indicates the correlation between water retention and film-forming properties, and also shows that a hydrogel with good film-forming properties can quickly isolate the internal and external environments, reduce water volatilization, and thus improve the water retention performance of the material. The water retention of Comparative Example 2 is higher than that of Comparative Example 1, proving that salidroside promotes macromolecular cross-linking, improves film-forming properties, and enhances water retention.

[0073] IV. Water Solubility Test

[0074] Weigh 0.2 g of the test sample after it is completely dried and record it as M0. Place the completely dried sample in deionized water to swell for 4 hours, take it out, and then dry it to a constant weight, and record it as M s . Calculate the water solubility according to formula (2).

[0075]

[0076] The measurement results are as Figure 4As shown. The order of water solubility from high to low is Example 2, Comparative Example 1, Comparative Example 2, Example 1, Example 3, Comparative Example 3. This indicates that the higher the proportion of the functional solution, the higher the solubility of the hydrogel. The solubility of Comparative Example 1 is higher than that of Comparative Example 2, again indicating that salidroside promotes molecular cross-linking, forms a macromolecular aggregation domain, and reduces water solubility.

[0077] V. Rheological properties

[0078] The rheological properties of samples with different contents were measured using a rheometer (Fluiddi CAM Rheo, France) at 25 °C in the shear rate range of 500 to 1000 s -1 .

[0079] The measurement results are as Figure 5 shown. The order of the viscosity of the hydrogel from high to low is Example 3, Example 1, Comparative Example 2, Comparative Example 1, Example 2 (the rheological characteristics of Comparative Example 3 could not be accurately measured because it presented a dispersed flocculent inhomogeneous system). This viscosity change is basically consistent with the change trend of film-forming property. On the one hand, it indicates that there is a positive correlation between viscosity and film-forming property; on the other hand, it once again proves the existence of the synergistic mechanism of the interpenetration of the network skeleton and the functional solution to form chain entanglement. To a certain extent, the multifunctional solution reduces the viscosity of the sample, reflecting that the multifunctional solution plays a role in balancing the film-forming property and multifunctionality of the present invention. The viscosity of Comparative Example 2 is higher than that of Comparative Example 1, once again proving the existence of the promoting molecular cross-linking effect of salidroside.

[0080] It shows that the hand care hydrogel prepared by the present invention belongs to a typical pseudoplastic fluid, and the shear thinning behavior conforms to the power-law model (η = Kγ˙ n-1 , where n < 1). The shear thinning characteristic of this solution matches the thixotropy required for hand cream - high viscosity at rest (to prevent leakage) and low viscosity during application (easy to spread). Stronger shear thinning at low concentrations may bring a lighter skin feel, which is suitable for the design of hand cream pursuing "quick absorption". When this product is applied on the skin surface, water is volatilized through body temperature, and the concentration is increased to above the specific concentration threshold of the hydrogel to form a gel (such as the critical cross-linking concentration). Combined with the support of the network skeleton, the film-forming property of this product can be further improved.

[0081] VI. Antioxidant property

[0082] The antioxidant property of the material was determined by DPPH and ABTS free radical capture. The specific measurement methods are as follows:

[0083] 1. DPPH

[0084] (1) Preparation of DPPH reagent: Weigh 5.0 mg of DPPH, dissolve it in an appropriate amount of absolute ethanol, and ultrasonicate it in the dark until it is fully dissolved. Then, make up the volume to 100 mL with absolute ethanol to prepare a 50 μg / mL DPPH solution (this solution should be prepared and used immediately).

[0085] (2) Preparation of the mother liquor of the sample to be tested: Weigh 10 mg of the sample, dissolve it in distilled water and make up the volume to 1 mL to prepare a 10 mg / mL mother liquor.

[0086] (3) Detection steps: Take 3 test tubes and label them as No. 1, No. 2, and No. 3. Add 3 mL of DPPH solution and 1 mL of the mother liquor of the sample to be tested to the first test tube as the experimental group (A s ); add 1 mL of the mother liquor of the sample to be tested and 3 mL of absolute ethanol solution to the second test tube as the control group (A c ); add 3.0 mL of DPPH solution and 1.0 mL of distilled water to the third test tube as the blank group (A b ). Mix them well respectively. After reacting in the dark at room temperature for 30 min, under the condition of a wavelength of 517 nm, use a UV-visible spectrophotometer to zero and calibrate with the sample solvent to measure the absorbance value. Calculate the antioxidant property according to formula (3).

[0087]

[0088] 2. ABTS

[0089] (1) Preparation of ABTS reagent: Weigh 200 mg of ABTS and 34.4 mg of potassium persulfate, dissolve them in 50 mL of distilled water, shake well, and let it stand in the dark at room temperature for 24 h to obtain the ABTS mother liquor. After overnight, take 0.5 mL of the ABTS mother liquor and dilute it to an absorbance value in the range of 0.70 ± 0.02 with 50 mL of 95% ethanol to obtain the ABTS test solution (this solution should be prepared and used immediately).

[0090] (2) Preparation of the mother liquor of the sample to be tested: Weigh 10 mg of the sample, dissolve it in distilled water and make up the volume to 1 mL to prepare a 10 mg / mL mother liquor.

[0091] (3) Detection steps: Take 2 test tubes and label them as No. 1 and No. 2. Add 3.6 mL of ABTS solution and 0.4 mL of the mother liquor of the sample to be tested to the first test tube as the experimental group (A s ); add 3.6 mL of ABTS solution and 0.4 mL of the sample solvent solution to the second test tube as the blank group (A b ). Mix them well. After reacting in the dark at room temperature for 5 min, under the condition of a wavelength of 734 nm, use a UV-visible spectrophotometer to zero and calibrate with the sample solvent to measure the absorbance value. Calculate the antioxidant property according to formula (4).

[0092]

[0093] The measurement results are as follows Figure 6 shown. The changing trends of the antioxidant properties of the hydrogels measured by the two methods are basically the same. The order from high to low is Example 2, Comparative Example 2, Example 1, Example 3, Comparative Example 1, and Comparative Example 3. This changing order is basically the same as the changing trend of the salidroside concentration, fully proving the excellent antioxidant properties of salidroside. It should be noted that: the salidroside concentrations in Comparative Example 2 and Example 1 are the same, but the antioxidant property of Comparative Example 2 is significantly better than that of Example 1, indicating that the degree of manifestation of the antioxidant property of salidroside is closely related to the synergistic effect of other components. This also indirectly verifies that the antioxidant groups of salidroside are cross-linked with other molecules, thereby weakening its own antioxidant property.

[0094] The above detailed description of a multifunctional hand-care hydrogel based on a chitosan / hyaluronic acid skeleton network and its preparation method with reference to the examples is illustrative rather than restrictive. Several examples can be listed according to the defined scope. Therefore, changes and modifications within the general concept of the present invention should fall within the protection scope of the present invention.

Claims

1. A multifunctional hand-care hydrogel based on a chitosan / hyaluronic acid skeleton network, characterized in that, The multifunctional hand care hydrogel is composed of a chitosan / hyaluronic acid skeleton network material and a multifunctional solution; The multifunctional solution comprises raw materials in the following parts by weight: 0.5 - 1 part of sodium hyaluronate, 0.001 - 0.005 part of essential oil, 0.05 - 0.5 part of xanthan gum, 0.1 - 0.5 part of gelatin, 0.0005 - 0.002 part of benzalkonium chloride, 0.05 - 0.2 part of salidroside, and 100 - 150 parts of water.

2. The multifunctional hand-care hydrogel based on a chitosan / hyaluronic acid backbone network according to claim 1, wherein The mass ratio of the chitosan / hyaluronic acid skeleton network material to the multifunctional solution is 1:10 - 50.

3. The multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network according to claim 1, characterized in that, The preparation method of the chitosan / hyaluronic acid skeleton network material comprises the following steps: (1) Heat and stir sodium hyaluronate powder in water until completely dissolved, and then add chitosan powder and stir to make it evenly dispersed; (2) Dropwise add glacial acetic acid solution to the mixture in step (1) until the chitosan is completely dissolved, and continue to stir until evenly mixed; (3) Let the reaction solution obtained in step (2) stand at room temperature for 2 - 24 h; (4) Separate and obtain the precipitate in step (3); (5) Add deionized water to the precipitate obtained in step (4), stir to make it evenly dispersed, stand at room temperature for 2 - 24 h, and separate to obtain a precipitate; (6) Repeat the washing of the precipitate obtained in step (5) twice according to step (5), separate the precipitate, and obtain the chitosan / hyaluronic acid skeleton network material.

4. The multifunctional hand-care hydrogel based on the chitosan / hyaluronic acid backbone network according to claim 3, characterized in that, In step (1), the mass ratio of sodium hyaluronate to chitosan is 1 - 3:2; the mass concentration of sodium hyaluronate in water is 0.05 - 2%.

5. The multifunctional hand care hydrogel based on the chitosan / hyaluronic acid backbone network according to claim 3, characterized in that In step (2), the mass concentration of glacial acetic acid is 0.5 - 5%; when dropping the glacial acetic acid solution, the volume of the added glacial acetic acid solution is 20 - 50% of the volume of water, and the temperature of the system is 40 - 50°C.

6. The multifunctional hand care hydrogel based on the chitosan / hyaluronic acid framework network according to claim 3, wherein In step (5), the mass ratio of water to the precipitate is 1 - 10:

1.

7. The multifunctional hand care hydrogel based on a chitosan / hyaluronic acid skeleton network according to claim 3, wherein, The preparation method of the multifunctional solution is: Heat and stir sodium hyaluronate in water until completely dissolved, then add essential oil and stir evenly; add xanthan gum and stir until completely dissolved; Add gelatin solution and stir evenly; finally add benzalkonium chloride solution and salidroside solution and stir evenly to form a multifunctional solution.

8. The multifunctional hand care hydrogel based on a chitosan / hyaluronic acid backbone network according to claim 7, wherein The temperature of the heat and stir is 45 - 55°C.

9. The preparation method of the multifunctional hand care hydrogel based on the chitosan / hyaluronic acid skeleton network according to any one of claims 1-8, characterized in that, The preparation method comprises the following steps: Disperse the chitosan / hyaluronic acid skeleton network material into the multifunctional solution, stir and mix at 35 - 45°C for at least 30 min, ultrasonicate for 20 - 60 min, stand at 60°C for 30 min respectively, and then stand at 4°C for 90 min, and alternate and cycle 3 times.

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