Method for simultaneously extracting tea saponin and flavone

Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for simultaneously extracting tea saponin and flavone. The method comprises the following steps: soaking tea seed meal in an ethanol solution, extracting in an ultrasonic extraction instrument after soaking, and filtering to obtain a crude extracting solution; drying the crude extract to obtain crude extract powder, and dissolving the crude extract powder in an ethanol solution to obtain a crude extract solution; soaking pretreated AB-8 macroporous resin in the crude extract solution, and performing ultrasonic treatment, filtration and drying to obtain tea saponin; soaking the soaked AB-8 macroporous resin in an ethanol solution, and performing ultrasonic treatment, filtration and drying to obtain flavone; the method adopts an ultrasonic-assisted ethanol extraction technology, and has the characteristics of economy, greenness, high efficiency, completeness and the like; the tea saponin and flavone with the purity greater than 95% are obtained by adopting AB-8 macroporous resin in a two-step manner, and the method has the characteristics of high yield, simplicity in operation, complete separation and the like.

Description

Technical Field

[0001] This application belongs to the technical field of plant extraction, and specifically relates to a method for simultaneously extracting tea saponin and flavonoids. Background Art

[0002] Tea saponin has broad application prospects, but its extraction and purification process faces many challenges. Traditional extraction methods, such as water extraction method, although simple in operation, due to the complex leaching components, it increases the difficulty of subsequent separation and purification, and has high energy consumption. On the other hand, although the organic solvent extraction method can improve the extraction efficiency, there are safety and cost problems. Especially the methanol extraction method is not suitable for large-scale production due to the high toxicity of methanol. These factors limit the efficient purification of tea saponin and its wider industrial application. Summary of the Invention

[0003] The purpose of the present invention is to solve the deficiencies of the prior art and provide a method for simultaneously extracting tea saponin and flavonoids. The specific technical solutions are as follows: In the first aspect, the present invention provides a method for simultaneously extracting tea saponin and flavonoids, comprising the following steps: S1. Soak tea meal in an ethanol solution, and after the soaking is completed, place it in an ultrasonic extractor for extraction, and then filter to obtain a crude extract; S2. Dry the crude extract to obtain a crude extract powder, and dissolve it in an ethanol solution to obtain a crude extract solution; the concentration of the ethanol solution is 5% - 20%; S3. Soak the pretreated AB-8 macroporous resin in the crude extract solution, perform ultrasonic treatment, filter, and dry to obtain tea saponin; S4. Soak the AB-8 macroporous resin after soaking in S3 in an ethanol solution, perform ultrasonic treatment, filter, and dry to obtain flavonoids; The concentration of the ethanol solution is 34% - 67%.

[0004] In view of the defects of the current extraction and purification technologies, such as being discontinuous, complex purification operations, and low yields, the present invention provides a comprehensive extraction and purification method for simultaneously obtaining tea saponin and flavonoids with a purity greater than 95% from extraction. The present invention extracts tea saponin and flavonoids by adopting ultrasonic combined with macroporous resin adsorption technology. Among them, on the one hand, based on the cavitation effect, mechanical effect, and thermal effect generated by ultrasonic waves, it can accelerate the release, diffusion, and dissolution of substances inside cells, thus significantly improving the extraction efficiency. Compared with traditional extraction methods, ultrasonic extraction not only increases the extraction rate but also can be carried out at a lower temperature, reducing the damage to heat-sensitive components. On the other hand, through the physical adsorption mechanism of macroporous resin, it binds to target molecules relying on van der Waals forces or hydrogen bonds. Since the resin has pore structures of different sizes inside, it can effectively screen out compounds within the required molecular weight range. In the present invention, the AB-8 macroporous resin is immersed in the crude extract solution to be extracted, and the solution passes through a column containing the macroporous resin, and the target component (flavonoid) therein is selectively adsorbed, while non-target impurities flow out with the mobile phase. Then, by changing the elution conditions (the concentration of the ethanol solution), the adsorbed target component can be desorbed to achieve the purpose of separation and purification.

[0005] As a further preferred embodiment, the dosage ratio of the tea meal to the ethanol solution in S1 is 1:5 to 1:15.

[0006] As a further preferred embodiment, the ultrasonic time in S1 is 0.5 h to 1.5 h; the ultrasonic power is 1000 w to 1600 w.

[0007] As a further preferred embodiment, the drying in S2 is at least one of spray drying, vacuum freeze drying, and rotary evaporation drying.

[0008] As a further preferred embodiment, the concentration of the crude extract in the crude extract solution is 10 g / L to 70 g / L.

[0009] As a further preferred embodiment, the volume ratio of the crude extract solution to the AB-8 macroporous resin in S3 is 1:3 to 1:20; the ultrasonic power in S3 is 200 w.

[0010] As a further preferred embodiment, the adsorption time of the AB-8 macroporous resin immersed in the crude extract solution is 2 h to 24 h.

[0011] As a further preferred embodiment, the pretreatment steps of the AB-8 macroporous resin include: first, immersing the AB-8 macroporous resin in absolute ethanol for activation, and then placing it in water for wetting to obtain the pretreated AB-8 macroporous resin.

[0012] As a further preferred embodiment, the volume ratio of the ethanol solution to the AB-8 macroporous resin in S4 is 1:3 to 1:20.

[0013] As a further preferred embodiment, the desorption of the AB-8 macroporous resin in S4 is 2 h to 24 h; the power of the ultrasound in S4 should be 200 w.

[0014] The beneficial effects of the present invention are as follows: (1) The present invention adopts the ultrasonic-assisted ethanol extraction technology, which relies on the cavitation effect, mechanical effect, and thermal effect to quickly and effectively promote the dissolution of natural products in the extraction solution. By setting a specific ethanol concentration, impurities in the extraction solution can also be reduced, and it has the characteristics of economy, greenness, high efficiency, and completeness; (2) The AB-8 macroporous resin adopted in the present invention has different adsorption forces for flavonoids and tea saponins. By adjusting the ethanol concentration in the aqueous solution of the crude extract, the adsorption force of the macroporous resin for flavonoids is strengthened, and tea saponins and flavonoids are separated more simply, completely, and greenly; (3) The purification and separation operation involved in the present invention only has two steps: the macroporous resin adsorbs flavonoids to obtain tea saponins with a purity greater than 95%, and the adsorbed flavonoids with a purity greater than 95% are eluted with an ethanol-aqueous solution. The lower-frequency ultrasound can vibrate the macroporous resin, increasing the contact area with the solution. Compared with other published technologies, the step of eluting tea saponins from the macroporous resin is reduced, greatly simplifying the process; (4) In the method provided by the present invention, the crude extract is first spray-dried into powder and then redissolved with ethanol-water. The contents of tea saponins and flavonoids in the solution are much higher than those in the crude extract. The content of flavonoids in the crude extract is relatively low, while the adsorption capacity of the macroporous resin is relatively large. Therefore, a certain volume of macroporous resin can process and separate more high-purity tea saponins and flavonoids, and the yield is very considerable. Description of the Drawings

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the following-described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0016] Figure 1 Shown is the process flow chart.

[0017] Figure 2 Shown are the liquid phase signal diagrams of crude tea saponins, high-purity tea saponins, and high-purity flavonoids before and after purification. Detailed Embodiments

[0018] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present application without creative efforts shall fall within the protection scope of the present application.

[0019] Example 1 A method for simultaneously extracting tea saponin and flavonoids specifically includes the following steps: (1) Extraction: Mix the crushed tea seed cake with a 75% ethanol solution and then put it into an ultrasonic extraction instrument for extraction (the mixing ratio of tea seed cake to ethanol solution is 1:5, w / v), the ultrasonic frequency is 1000 w, the ultrasonic time is 0.5 h, then filter, collect the crude extract, and discard the filter residue; (2) Spray drying: Use a spray dryer to make the crude extract obtained in step (1) into a crude extract powder; (3) Extracting tea saponin: Mix the crude extract powder in step (2) with an ethanol solution (the mass concentration of the crude extract solution is 10 g / L, and the concentration of ethanol in the ethanol solution is 5%) to obtain a crude extract solution; then soak AB-8 macroporous resin in absolute ethanol (the mass ratio of absolute ethanol to AB-8 macroporous resin is 2:1) for activation for 24 h, recover the absolute ethanol, then soak it in water (the mass ratio of water to AB-8 macroporous resin is 2:1) for 2 h, and then discard the water. The obtained wet macroporous resin is reserved; Soak the pretreated AB-8 macroporous resin in the crude extract solution for adsorption (the volume ratio of AB-8 macroporous resin to the crude extract solution is 1:4), the adsorption time is 2 h, ultrasonic-assisted oscillation at 200 w, filter and collect the filtrate, and the tea saponin is obtained after drying the filtrate; (4) Extracting flavonoids: Soak the AB-8 macroporous resin intercepted in step (3) in an ethanol solution (the concentration of ethanol in the ethanol solution is 34%), the volume ratio of AB-8 macroporous resin to the ethanol solution is 1:4, the desorption time is 2 h, ultrasonic-assisted oscillation at 200 w, filter and collect the filtrate, and the flavonoids are obtained after drying the filtrate.

[0020] Example 2 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this embodiment is similar to that of Example 1, and the only difference is that "the mixing ratio of tea seed cake to ethanol solution is 1:5" in step (1) is replaced by "the mixing ratio of tea seed cake to ethanol solution is 1:10".

[0021] Example 3 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the mixing ratio of tea meal to ethanol solution is 1:5" in step (1) is replaced with "the mixing ratio of tea meal to ethanol solution is 1:15".

[0022] Example 4 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the ultrasonic frequency is 1000 w" in step (1) is replaced with "the ultrasonic frequency is 1200 w".

[0023] Example 5 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the ultrasonic frequency is 1000 w" in step (1) is replaced with "the ultrasonic frequency is 1500 w".

[0024] Example 6 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the ultrasonic time is 0.5 h" in step (1) is replaced with "the ultrasonic time is 1 h".

[0025] Example 7 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the ultrasonic time is 0.5 h" in step (1) is replaced with "the ultrasonic time is 1.5 h".

[0026] Example 8 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the concentration of ethanol in the ethanol solution is 5%" in step (3) is replaced with "the concentration of ethanol in the ethanol solution is 10%". Example 9 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the concentration of ethanol in the ethanol solution is 5%" in step (3) is replaced with "the concentration of ethanol in the ethanol solution is 20%". Example 10 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the mass concentration of the crude extract solution is 10 g / L" in step (3) is replaced with "the mass concentration of the crude extract solution is 30 g / L".

[0027] Example 11 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the mass concentration of the crude extract solution is 10 g / L" in step (3) is replaced with "the mass concentration of the crude extract solution is 50 g / L".

[0028] Example 12 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the volume ratio of AB-8 macroporous resin to the crude extract solution is 1:4" in step (3) is replaced with "the volume ratio of AB-8 macroporous resin to the crude extract solution is 1:8".

[0029] Example 13 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the volume ratio of AB-8 macroporous resin to the crude extract solution is 1:4" in step (3) is replaced with "the volume ratio of AB-8 macroporous resin to the crude extract solution is 1:20".

[0030] Example 14 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the adsorption time is 2 h" in step (3) is replaced with "the adsorption time is 8 h".

[0031] Example 15 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the adsorption time is 2 h" in step (3) is replaced with "the adsorption time is 24 h".

[0032] Example 16 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the concentration of ethanol in the ethanol solution is 34%" in step (4) is replaced with "the concentration of ethanol in the ethanol solution is 50%".

[0033] Example 17 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the concentration of ethanol in the ethanol solution is 34%" in step (4) is replaced with "the concentration of ethanol in the ethanol solution is 65%".

[0034] Example 18 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that "the volume ratio of AB-8 macroporous resin to the ethanol solution is 1:4" in step (4) is replaced with "the volume ratio of AB-8 macroporous resin to the ethanol solution is 1:8".

[0035] Example 19 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that in step (4), "the volume ratio of AB-8 macroporous resin to ethanol solution is 1:4" is replaced with "the volume ratio of AB-8 macroporous resin to ethanol solution is 1:20".

[0036] Example 20 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that in step (4), "the elution time is 2 h" is replaced with "the elution time is 12 h".

[0037] Example 21 A method for simultaneously extracting tea saponin and flavonoids. The specific process of this example is similar to that of Example 1, and the only difference is that in step (4), "the elution time is 2 h" is replaced with "the elution time is 24 h".

[0038] Example 22 In this example, Examples 1 - 3 were used as experimental objects to explore the effect of the mixing ratio of tea seed meal to ethanol solution during extraction on the yield of tea saponin. The specific process is as follows: Experimental method: The liquid chromatography method with an ELSD detector was used, and the peak area method was used to calculate the yield of tea saponin. The mobile phase was 0.1% formic acid water and acetonitrile. It was 10% acetonitrile from 0 to 3 min and 40% acetonitrile from 3 min to 7 min; the single injection volume was 10 μL; the column temperature was 30°C; the nebulizer temperature was 75°C; the drift tube temperature was 80°C; the nitrogen flow rate was 1.6 L / min; standard tea saponin solutions with mass concentrations of 0.3 mg / mL, 0.5 mg / mL, 0.7 mg / mL, 1.0 mg / mL, 1.3 mg / mL, 1.5 mg / mL, 1.7 mg / mL, and 2.0 mg / mL were prepared, a mass concentration - peak area graph was made, and the standard curve formula was calculated.

[0039] When the mixing ratios of Examples 1 - 3 were 1:5, 1:10, and 1:15, the yields of tea saponin were 16%, 21%, and 21% in sequence.

[0040] Example 23 In this example, Examples 1, 4, and 5 were used as experimental objects to explore the effect of ultrasonic frequency during extraction on the yield of tea saponin. The process is as follows: Experimental method: The liquid chromatography method with an ELSD detector was used, and the peak area method was used to calculate the yield of tea saponin.

[0041] When the ultrasonic frequencies of Examples 1, 4, and 5 are 1000 w, 1200 w, and 1500 w respectively, the yields of tea saponin are 18%, 21%, and 21% in sequence.

[0042] Example 24 In this example, Examples 1, 6, and 7 were used as experimental objects to explore the influence of ultrasonic time during extraction on the yield of tea saponin. The process is as follows: Experimental method: The liquid chromatography method with an ELSD detector was used, and the peak area method was adopted to calculate the yield of tea saponin.

[0043] When the ultrasonic times of Examples 1, 6, and 7 are 0.5 h, 1 h, and 1.5 h respectively, the yields of tea saponin are 15%, 19%, and 21% in sequence.

[0044] Example 25 In this example, Examples 1, 8, and 9 were used as experimental objects to explore the influence of ethanol concentration during adsorption on the purity of tea saponin. The process is as follows: Experimental method: The liquid chromatography method with an ELSD detector was used, and the peak area method was adopted to calculate the yield of tea saponin. The mobile phase was 0.1% formic acid water and acetonitrile. It was 10% acetonitrile from 0 to 3 min and 40% acetonitrile from 3 min to 7 min; the single injection volume was 10 μL; the column temperature was 30°C; the nebulizer temperature was 75°C; the drift tube temperature was 80°C; the nitrogen flow rate was 1.6 L / min; standard tea saponin solutions with mass concentrations of 0.3 mg / mL, 0.5 mg / mL, 0.7 mg / mL, 1.0 mg / mL, 1.3 mg / mL, 1.5 mg / mL, 1.7 mg / mL, and 2.0 mg / mL were prepared, and a mass concentration - peak area graph was made and the standard curve formula was calculated.

[0045] When the ethanol concentrations of Examples 1, 8, and 9 are 5%, 10%, and 20% respectively, the purities of tea saponin are 76%, 78%, and 73% in sequence.

[0046] Example 26 In this example, Examples 1, 10, and 11 were used as experimental objects to explore the influence of the mass concentration of the crude extract solution during adsorption on the purity of tea saponin. The process is as follows: Experimental method: The liquid chromatography method with an ELSD detector was used, and the peak area method was adopted to calculate the purity of tea saponin.

[0047] When the mass concentrations of the crude extract solutions of Examples 1, 10, and 11 are 10 g / L, 30 g / L, and 50 g / L respectively, the purities of tea saponin are 84%, 83%, and 82% in sequence.

[0048] Example 27 In this example, Examples 1, 12, and 13 were used as experimental objects to explore the effect of the ratio of macroporous resin to solution on the purity of saponins during adsorption. The process is as follows: Experimental method: High performance liquid chromatography with an evaporative light scattering detector (ELSD) was used, and the peak area method was adopted to calculate the purity of saponins.

[0049] When the ratios of Examples 1, 12, and 13 were 1:4, 1:8, and 1:20 respectively, the purities of saponins were 91%, 83%, and 75% in sequence.

[0050] Example 28 In this example, Examples 1, 14, and 15 were used as experimental objects to explore the effect of adsorption time on the purity of saponins. The process is as follows: Experimental method: High performance liquid chromatography with an evaporative light scattering detector (ELSD) was used, and the peak area method was adopted to calculate the purity of saponins.

[0051] When the adsorption times of Examples 1, 14, and 15 were 2 h, 8 h, and 24 h respectively, the purities of saponins were 84%, 95%, and 97% in sequence.

[0052] Example 29 In this example, Examples 1, 16, and 17 were used as experimental objects to explore the effect of ethanol concentration on the purity of flavonoids during desorption. The process is as follows: Experimental method: High performance liquid chromatography with an evaporative light scattering detector (ELSD) was used, and the peak area method was adopted to calculate the purity of flavonoids. The mobile phase was 0.1% formic acid in water and acetonitrile. The acetonitrile concentration was 10% from 0 to 3 min and 40% from 3 to 7 min; the injection volume was 10 μL; the column temperature was 30°C; the nebulizer temperature was 75°C; the drift tube temperature was 80°C; the nitrogen flow rate was 1.6 L / min; rutin standard solutions with mass concentrations of 0.3 mg / mL, 0.5 mg / mL, 0.7 mg / mL, 1.0 mg / mL, 1.3 mg / mL, 1.5 mg / mL, 1.7 mg / mL, and 2.0 mg / mL were prepared, and a mass concentration - peak area graph was made to calculate the standard curve formula.

[0053] When the ethanol concentrations of Examples 1, 16, and 17 were 34%, 50%, and 65% respectively, the purities of flavonoids were 79%, 94%, and 92% in sequence.

[0054] Example 30 In this example, Examples 1, 18, and 19 were used as experimental objects to explore the effect of the ratio of macroporous resin to solution on the purity of flavonoids during desorption. The process is as follows: Experimental method: High performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) was used, and the purity of flavonoids was calculated by peak area method.

[0055] When the ratios of Example 1, Example 18 and Example 19 were 1:4, 1:8, 1:20, the purities of flavonoids were 94%, 95%, 95% in sequence.

[0056] Example 31 In this example, Example 1, Example 20 and Example 21 were taken as experimental objects to explore the influence of analysis time on the purity of flavonoids. The process is as follows: Experimental method: High performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) was used, and the purity of flavonoids was calculated by peak area method.

[0057] When the analysis times of Example 1, Example 20 and Example 21 were 2 h, 12 h, 24 h, the purities of flavonoids were 43%, 91%, 96% in sequence.

[0058] Example 32 A method for simultaneously extracting tea saponin and flavonoids specifically comprises the following steps: (1) Extraction: The crushed tea seed meal was mixed with 75% ethanol solution and then put into an ultrasonic extraction instrument for extraction (the mixing ratio of tea seed meal to ethanol solution was 1:10, w / v), the ultrasonic frequency was 1200 w, the ultrasonic time was 1.5 h, and then filtered to collect the crude extract, and the filter residue was discarded (the yield was 21%); (2) Spray drying: The crude extract obtained in step (1) was made into a crude extract powder by a spray dryer; (3) Extracting tea saponin: The crude extract powder in step (2) was mixed with ethanol solution (the mass concentration of the crude extract solution was 10 g / L, and the concentration of ethanol in the ethanol solution was 10%) to obtain a crude extract solution; then AB-8 macroporous resin was soaked in absolute ethanol (the mass ratio of absolute ethanol to AB-8 macroporous resin was 2:1) for activation for 24 h, the absolute ethanol was recovered, and then soaked in water (the mass ratio of water to AB-8 macroporous resin was 2:1) for 2 h, and then the water was discarded to obtain the wet macroporous resin for standby; the pretreated AB-8 macroporous resin was soaked in the crude extract solution for adsorption (the volume ratio of AB-8 macroporous resin to the crude extract solution was 1:4), the adsorption time was 24 h, and ultrasonic assisted oscillation was carried out at 200 w. After filtration, the filtrate was collected, and after drying, tea saponin was obtained (the purity of tea saponin was 97%, as Figure 2 shown); (4) Extracting flavonoids: The AB-8 macroporous resin intercepted in step (3) was soaked in ethanol solution (the concentration of ethanol in the ethanol solution was 50%), the volume ratio of AB-8 macroporous resin to ethanol solution was 1:8, the analysis time was 24 h, and ultrasonic assisted oscillation was carried out at 200 w. After filtration, the filtrate was collected, and after drying, flavonoids were obtained (the purity of flavonoids was 96%, as Figure 2as shown).

[0059] The embodiments of the present application have been described above in conjunction with the accompanying drawings. Specific examples have been used herein to elaborate on the principles and implementation manners of the present application. The description of the above embodiments is only used to help understand the core idea of the present application. However, the present application is not limited to the above specific implementation manners. The above specific implementation manners are merely illustrative and not restrictive. Under the inspiration of the present application, those of ordinary skill in the art can also make many forms without departing from the purpose of the present application and the scope protected by the claims, and all of them fall within the protection scope of the present application.

Claims

1. A method for simultaneously extracting tea saponin and flavonoids, characterized in that, It includes the following steps: S1. Soak tea seed cake in an ethanol solution, and after the soaking is completed, place it in an ultrasonic extractor for extraction, and then filter to obtain a crude extract; S2. Dry the crude extract to obtain a crude extract powder, and dissolve it in an ethanol solution to obtain a crude extract solution; the concentration of the ethanol solution is 5% - 20%; S3. Soak the pretreated AB-8 macroporous resin in the crude extract solution, perform ultrasonic treatment, filter, and dry to obtain tea saponin; S4. Soak the AB-8 macroporous resin after soaking in S3 in an ethanol solution, perform ultrasonic treatment, filter, and dry to obtain flavonoids; The concentration of the ethanol solution is 34% - 67%.

2. The method according to claim 1, wherein The dosage ratio of the tea seed cake to the ethanol solution in S1 is 1:5 - 1:

15.

3. The method according to claim 1, wherein The ultrasonic time in S1 is 0.5 h - 1.5 h; the ultrasonic power is 1000 w - 1600 w.

4. The method according to claim 1, wherein The drying in S2 is at least one of spray drying, vacuum freeze drying, and rotary evaporation drying.

5. The method according to claim 1, characterized in that, The concentration of the crude extract in the crude extract solution in S2 is 10 g / L - 70 g / L.

6. The method according to claim 1, wherein The volume ratio of the crude extract solution to the AB-8 macroporous resin in S3 is 1:3 - 1:20; the ultrasonic power in S3 is 200 w.

7. The method according to claim 6, wherein The adsorption time of the AB-8 macroporous resin soaked in the crude extract solution is 2 h - 24 h.

8. The method according to claim 1, characterized in that, The pretreatment steps of the AB-8 macroporous resin include: First, soak the AB-8 macroporous resin in absolute ethanol for activation, and then place it in water for wetting to obtain the pretreated AB-8 macroporous resin.

9. The method according to claim 8, wherein The volume ratio of the ethanol solution to the AB-8 macroporous resin in S4 is 1:3 - 1:

20.

10. The method according to claim 9, wherein The desorption of the AB-8 macroporous resin in S4 is 2 h - 24 h; the ultrasonic power in S4 should be 200 w.

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