Composition for controlling oil and maintaining scalp micro-ecology, application and cosmetic

Abstract

The invention belongs to the field of daily chemicals, and discloses a composition for controlling oil and maintaining scalp micro-ecology, which comprises a solution containing sericin peptide, a prebiotic composition and polylysine, the weight ratio of the solution containing the sericin peptide to the prebiotic composition to the polylysine is (3-20): (5-10): (0.1-1). According to the composition, polylysine and prebiotics (inulin and fructo-oligosaccharide) are combined with sericin protein peptide (containing lysozyme), better oil control performance is achieved, meanwhile, the functions of moisturizing, barrier repairing and the like of the sericin protein peptide are utilized, a complete skin barrier is established while harmful bacteria are controlled, and micro-ecology repairing and improving are achieved. Meanwhile, the invention further provides application and cosmetics of the composition.

Description

Technical Field

[0001] The present invention relates to the daily chemical field, and particularly to a composition for controlling oil and maintaining scalp microecology. Background Art

[0002] Lysozyme and polylysine are two relatively classic antibacterial agents in cosmetics, and they are widely used for antibacterial purposes, as shown in the following literature: The publication number is CN118453445A, and the theme is a prebiotic oral care antibacterial agent and its preparation method. It discloses a prebiotic oral care antibacterial agent, which contains water, fructooligosaccharide, polydextrose, inulin, stachyose, tea polyphenols, ε-polylysine hydrochloride, lysozyme, and flavoring agent. It further points out that the antibacterial components use ε-polylysine hydrochloride, lysozyme, and tea polyphenols. By adding a prebiotic solution prepared by mixing "inulin, fructooligosaccharide, polydextrose, stachyose" and pure water, the prebiotic can be utilized by beneficial bacteria such as Streptococcus salivarius in the oral cavity, enabling the proliferation of this bacterium into the dominant bacterium, thereby inhibiting the activity of harmful bacteria and improving the antibacterial property of the oral care antibacterial agent.

[0003] In the field of skin and hair care, it can be seen that the publication number is CN110917074A, and the theme is a composition with antibacterial effect and its application in additive-free skin care products. It discloses a composition with antibacterial effect. Through the appropriate selection of nisin, polylysine, and cordycepin, and the appropriate selection of the dosage, the prepared composition has good antibacterial and bacteriostatic effects, especially very good antibacterial and bacteriostatic effects on thermotolerant coliforms, Pseudomonas aeruginosa, Streptococcus albus, Aspergillus niger, and yeasts.

[0004] The purpose of this case is to develop a composition that can act on the maintenance and improvement of scalp microecology. Based on the related functions of lysozyme, polylysine, and prebiotics (inulin, fructooligosaccharide), we further develop on this basis. Summary of the Invention

[0005] The purpose of the present invention is to provide a composition for controlling oil and maintaining scalp microecology. The composition combines polylysine and prebiotics (inulin, fructooligosaccharide) with sericin protein peptide (containing lysozyme), achieving better oil control performance. At the same time, by utilizing the functions of sericin protein peptide such as moisturizing and barrier repair, while controlling harmful bacteria, a complete skin barrier is established, realizing the repair and improvement of microecology.

[0006] At the same time, the present invention also provides the application of the composition and cosmetics.

[0007] To achieve the above purpose, the present application discloses a composition for controlling oil and maintaining scalp microecology, including a solution containing sericin protein peptide, a prebiotic composition, and polylysine; The weight ratio of the sericin protein peptide-containing solution, prebiotic composition, and polylysine is 3 to 20:5 to 10:0.1 to 1; The content of sericin protein peptide in the sericin protein peptide-containing solution is 5 wt%. The product used in the present invention is a repair-type hydrolyzed sericin protein peptide product named InnoSeriCARE provided by MyoSource (Shanghai) Biotechnology Research Co., Ltd.

[0008] In the above composition, the prebiotic composition is fructooligosaccharide and / or inulin.

[0009] In the above composition, the prebiotic composition is a combination of fructooligosaccharide and inulin; the weight ratio of inulin to fructooligosaccharide is 1:10 to 1:1.

[0010] In the above composition, the weight ratio of inulin to fructooligosaccharide is 1:4 to 2:5.

[0011] Meanwhile, the present invention also discloses the use of the composition as described in any one of the above for preparing cosmetics.

[0012] Meanwhile, the present invention also discloses the use of the composition as described in any one of the above for preparing an antibacterial agent.

[0013] Finally, the present invention also discloses a cosmetic containing the composition as described in any one of the above.

[0014] In the above cosmetic, the cosmetic is shampoo, body wash or hair conditioner.

[0015] In the above cosmetic, the cosmetic contains 0.1% to 10% of the composition.

[0016] The beneficial effects of this application are: 1. By combining sericin protein peptide, prebiotic composition, and polylysine, through detection, it is found that when the three are combined, they have very excellent oil control effects; 2. The samples of the present invention were tested for antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Malassezia furfur. The test results confirmed that its antibacterial effect against Malassezia furfur is extremely strong, its antibacterial effect against Staphylococcus aureus is relatively strong, and it has no antibacterial effect against Staphylococcus epidermidis; this indicates that the composition of the present invention has good antibacterial effects and scalp microecological repair ability; 3. The samples of the present invention were tested for safety, proving that it is a mild product and can be used with confidence. Description of the Drawings

[0017] Figure 1 It is the cell activity test result of Example 1 of the present invention; Figure 2Cell viability test results of Example 2 of the present invention; Figure 3 Cell viability test results of Example 3 of the present invention; Figure 4 Cell viability test results of Comparative Example 1 of the present invention; Figure 5 Cell viability test results of Comparative Example 2 of the present invention; Figure 6 Cell viability test results of Comparative Example 3 of the present invention; Figure 7 Nile red staining fluorescence image of Example 1 of the present invention; Figure 8 Nile red staining fluorescence image of Example 2 of the present invention; Figure 9 Nile red staining fluorescence image of Example 3 of the present invention; Figure 10 Nile red staining fluorescence image of Comparative Example 1 of the present invention; Figure 11 Nile red staining fluorescence image of Comparative Example 2 of the present invention; Figure 12 Nile red staining fluorescence image of Comparative Example 3 of the present invention; Figure 13 Staphylococcus aureus antibacterial image of Example 2 of the present invention; Figure 14 Staphylococcus epidermidis antibacterial image of Example 2 of the present invention; Figure 15 Malassezia furfur antibacterial image of Example 1 of the present invention; Figure 16 Malassezia furfur antibacterial image of Example 2 of the present invention; Figure 17 Chorioallantoic membrane test image of Example 1 of the present invention; Figure 18 Chorioallantoic membrane test image of Example 2 of the present invention. Detailed implementation manners

[0018] The present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that for those conditions not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments whose manufacturers are not specified, they are all conventional products that can be obtained through commercial purchase.

[0019] Repair sericin protein peptide: Provided by Muscle Origin Collection (Shanghai) Biotechnology Research Co., Ltd., with the trade name InnoSeriCARE. The related preparation technology refers to CN112194715B Anti-inflammatory Sericin Protein Peptide and Its Application. The active ingredient of this product is sericin protein peptide, and the content of the active ingredient is 5%.

[0020] Polylysine, fructooligosaccharide, inulin: Provided by Guangzhou Hemiao Biotechnology Co., Ltd., and the effective content is 100% for all Part 1 Examples and Comparative Examples Mix polylysine, fructooligosaccharide, inulin, and hydrolyzed sericin protein and disperse them in water to form a homogeneous aqueous phase. The weight of each active ingredient in the aqueous phase refers to Table 1, and finally add water to make the total weight of the sample 100 g.

[0021] Table 1 Formulation Table (unit: g) Repair sericin protein peptide Inulin Fructooligosaccharide Polylysine Example 1 3 1 4 1 Example 2 8 2 5 0.5 Example 3 20 4 6 0.1 Comparative Example 1 20 0 0 0 Comparative Example 2 0 1 4 0 Comparative Example 3 0 0 0 1

[0022] Performance Detection I. Oil Control Performance Test Send the samples of Examples 1 - 3 and Comparative Examples 1 - 3 to Guangzhou Huamiao Biotechnology Research Institute Co., Ltd. for testing, and the test report numbers are 2504170 - 01A to 2504170 - 06A; Test Purpose and Principle SZ95 cells are an immortalized human facial sebaceous gland cell line. The excessive secretion of lipids by human sebaceous gland cells is a prerequisite for the occurrence of acne, and acne patients generally have strong facial sebaceous gland secretion. Inducing human sebaceous gland cells to secrete excessive lipids by linoleic acid can be used as a cell model for studying the oil control ability of cosmetics. After human sebaceous gland cells are induced by linoleic acid and administered with the test substance, the intracellular lipid deposition is observed under a microscope by Nile red staining, and the change in intracellular fluorescence intensity after the negative control and the test substance are administered is compared to evaluate whether the test sample has an effect in terms of oil control.

[0023] This test refers to the laboratory method. By inducing an increase in the intracellular lipid content of human sebaceous gland cells by linoleic acid, and comparing the change in the fluorescence intensity of the intracellular fluorescent dye after the negative control and the test sample are administered, the intracellular lipid synthesis inhibition rate is calculated. If the intracellular lipid content of the test sample is lower than that of the negative control and has a significant difference (P < 0.05), it can be considered that the test sample has an oil control effect.

[0024] Test Materials and Methods Instrument and Equipment: MF52 - N Inverted Fluorescence Microscope; Cells used in the experiment: Human sebaceous gland cells (SZ95 cells); Test Method (1) Treatment of Test Samples Sample group: Dilute the sample with pure water to a 4% solution, then filter it through a 0.22 μm filter, and collect the filtrate as the sample mother liquor.

[0025] (2) Test grouping Negative control group (NC): Cells (+) Linoleic acid (+) Nile red (+) Sample (-); Positive control group (PC): Cells (+) Linoleic acid (+) Nile red (+) Isotretinoin (+); Sample group (S): Cells (+) Linoleic acid (+) Nile red (+) Sample (+); (3) Test operation steps Cell viability assay: Seed human sebaceous gland cells in a 96-well plate. After 24 h, aspirate the culture medium, add the basal medium containing different concentrations of the test sample. After 24 h, detect the OD490nm by the MTT method, and analyze the effect of the test sample on the viability of human sebaceous gland cells by t-test.

[0026] Determination of intracellular lipid synthesis inhibition rate: Except for the blank group (BC) cell wells, add the culture medium containing a certain concentration of linoleic acid to the remaining wells, and place them in a CO2 incubator for induction for 6 h. After the test grouping and drug administration, place the plate in a CO2 incubator for 24 h. After incubation, discard the supernatant, add the prepared Nile red staining solution to the wells for cell staining, and take pictures under an inverted fluorescence microscope using the FITC channel, excitation wavelength 552 nm, objective lens 20 times, and set the same exposure time.

[0027] Calculation formula Intracellular lipid synthesis inhibition rate (%) = (1 - T / C) * 100%; Where: T - Average fluorescence intensity of the sample group / positive control; C - Average fluorescence intensity of the negative control group.

[0028] Test results Cell viability assay Before determining the intracellular lipid synthesis inhibition rate of the test sample, first perform cell viability screening to obtain the optimal test concentration; The cell viability test results of Examples 1 to 3 and Comparative Examples 1 to 3 can be referred to Figures 1 to 6 ; Nile red staining fluorescence images The Nile red staining fluorescence images of Examples 1 to 3 and Comparative Examples 1 to 3 can be referred to Figures 7 to 12 ; Oil control test results The experimental results are shown in Table 2; Table 2 Oil control test results Positive control group (PC) Sample group (0.125%) Sample group (0.25%) Example 1 59.90±1.15 14.13±2.51 11.87±1.30 Example 2 59.90±1.15 13.38±2.37 23.81±1.22 Example 3 59.60±1.84 4.40±1.85 25.28±1.32 Comparative Example 1 58.48±2.08 -3.30±1.52 5.68±2.77 Comparative Example 2 59.60±1.84 0.83±2.83 -0.45±1.76 Comparative Example 3 58.48±2.08 -9.36±1.51 -8.35±1.33

[0029] Result analysis: 1. As can be seen from Comparative Example 1 to Comparative Example 3, when each of the above components is used alone, there is no obvious oil control performance; 2. As can be seen from Example 1 to Example 3, when the amount of polylysine is very small (Example 3) and diluted to 0.125%, its oil control performance is significantly weaker than that of Example 1 and Example 2, indicating that when reducing the proportion of polylysine, the total formula concentration should be appropriately increased to ensure its full effectiveness; 3. As can be seen from Example 1 to Example 3, the combination of the present invention has a very good oil control effect.

[0030] II. Antibacterial test against Staphylococcus aureus Taking Example 2 as the sample, according to the evaluation method of antibacterial and bacteriostatic effects of daily chemical products QB / T 2738-2023, the bacteriostatic rate of the sample was tested, and the test report number was 2504111-02AR1; The test results show that: the blank control is <10 CFU / mL, the Staphylococcus aureus in the control sample group is 3.9*10 4 CFU / mL, the system is effective. When the sample is diluted into a 1% aqueous solution, the Staphylococcus aureus is 1.3*10 4 CFU / mL, and the inhibition rate is 66.67%, indicating that the product has antibacterial effect.

[0031] The test results can be seen in the appendix Figure 13 .

[0032] III. Antibacterial test against Staphylococcus epidermidis Taking Example 2 as the sample, according to the evaluation method of antibacterial and bacteriostatic effects of daily chemical products QB / T 2738-2023, the bacteriostatic rate of the sample was tested, and the test report number was 2504111-02BR1; The test results show that: the blank control is <10 CFU / mL, the Staphylococcus epidermidis in the control sample group is 2.0*10 4 CFU / mL, the system is effective. When the sample is diluted into a 1% aqueous solution, the Staphylococcus epidermidis is 1.1*10 4 CFU / mL, and the inhibition rate is 45.00%, indicating that the product has no antibacterial effect.

[0033] The test results can be seen in the appendix Figure 14 .

[0034] IV. Antibacterial test against Malassezia furfur Take Example 1 and Example 2 as samples for testing; the test report numbers are 2504111-02AR1 and 2504168-02AR1.

[0035] Purpose and Principle of the Experiment The problem of dandruff is a phenomenon of excessive exfoliation caused by the hyperplasia of scalp keratinocytes. Among them, Malassezia is one of the important factors causing dandruff. The increase in dandruff is a mild manifestation of scalp seborrheic dermatitis. Due to the proliferation of Malassezia, it leads to oily scalp and severe dandruff, and such a situation in turn promotes the proliferation of Malassezia. Therefore, adding an anti-dandruff agent to inhibit or kill such microorganisms has a positive effect on inhibiting the growth of dandruff; using Malassezia as the indicator bacterium, the anti-dandruff effect of shampoo products is tested and evaluated. This test method is an in vitro method and is applicable to the evaluation method for assessing the efficacy of claimed anti-dandruff cosmetics.

[0036] Experimental Method (1) Sample Treatment Sample: Dilute it with sterile standard hard water to a sample concentration of 0.5%; Blank Control: Phosphate buffer solution; Control Sample Group: Malassezia suspension + Phosphate buffer solution.

[0037] (2) Test Steps Take the Malassezia suspension, add it to each test sample solution and control sample solution respectively and mix evenly. Start timing. When the specified action time is reached, make appropriate dilutions. Take 2-3 dilution degrees, and respectively pipette the test sample solution and control sample solution into two petri dishes, pour the Pityrosporum medium and mix evenly. After it solidifies, turn the petri dish over and place it in the incubator for 5-10 days for viable count.

[0038] (3) Calculation Formula The inhibitory rate of Malassezia furfur = (A - B) / A * 100% Where: A - Average colony count of the control sample; B - Average colony count of the test sample.

[0039] The test result of Example 1 is: the blank control is <10 CFU / mL, the Malassezia furfur in the control sample group is 4.5 * 10 4 CFU / mL, the system is effective. When the sample is diluted into a 0.5% aqueous solution, the Malassezia furfur is 1.8 * 10 3 CFU / mL, and the inhibitory rate is 96.30%, indicating that the sample has a certain anti-dandruff efficacy. The test result graph of Example 1 can be seen Figure 15 .

[0040] The test results of Example 2 are as follows: the blank control is <10 CFU / mL, and the Malassezia furfur in the control sample group is 4.5*10 4 CFU / mL, the system is effective. When the sample is diluted into a 1% aqueous solution, the Malassezia furfur is 1.9*10 2 CFU / mL, and the inhibition rate is 99.58%, indicating that the sample has a certain anti-dandruff effect. The test result graph of Example 2 can be seen Figure 16 .

[0041] V. Irritation experiment The samples of Example 1 and Example 2 were subjected to the chicken embryo chorioallantoic membrane test, and the test report numbers were 2504168-01BR1 and 2504168-02BR1; The test results of Example 1 are as follows: Using the endpoint evaluation method, the negative control (0.9% normal saline) ES = 0.00, indicating that the sample is non-irritating and meets the standard of the negative control sample; the positive control (1% SDS) ES = 18.00, indicating that the sample is strongly irritating / corrosive and meets the standard of the positive control sample; when the sample is diluted to a 0.5% aqueous solution, ES = 2.33, indicating that this sample is non-irritating, indicating that the sample passes the mild non-irritating test.

[0042] The test results of Example 2 are as follows: Using the endpoint evaluation method, the negative control (0.9% normal saline) ES = 0.00, indicating that the sample is non-irritating and meets the standard of the negative control sample; the positive control (1% SDS) ES = 18.00, indicating that the sample is strongly irritating / corrosive and meets the standard of the positive control sample; when the sample is diluted into a 1% aqueous solution, ES = 2.00, indicating that this sample is non-irritating, indicating that the sample passes the mild non-irritating test.

[0043] Summary: 1. The formulation of the present invention shows through the oil control test that there is an obvious oil control synergy among the components of the formulation of the present invention.

[0044] 2. The samples of the present invention were subjected to antibacterial tests for Staphylococcus aureus, Staphylococcus epidermidis, and Malassezia furfur. The test results confirmed that its antibacterial effect on Malassezia furfur is extremely strong, its antibacterial effect on Staphylococcus aureus is relatively strong, and it has no antibacterial effect on Staphylococcus epidermidis; it shows that the composition of the present invention has good antibacterial effects and scalp microecological repair ability.

[0045] 3. The samples of the present invention were subjected to safety tests, proving that it is a mild product and can be used with confidence.

Claims

1. A composition for controlling oil and maintaining scalp microecology, characterized in that, Comprising a solution containing sericin protein peptides, a prebiotic composition, and polylysine; The weight ratio of the solution containing sericin protein peptides, the prebiotic composition, and polylysine is 3 to 20:5 to 10:0.1 to 1; The content of sericin protein peptides in the solution containing sericin protein peptides is 5 wt%.

2. The composition according to claim 1, wherein The prebiotic composition is fructooligosaccharide and / or inulin.

3. The composition according to claim 1, wherein The prebiotic composition is a combination of fructooligosaccharide and inulin; the weight ratio of inulin to fructooligosaccharide is 1:10 to 1:

1.

4. The composition according to claim 3, wherein The weight ratio of inulin to fructooligosaccharide is 1:4 to 2:

5.

5. Use of the composition according to any one of claims 1 to 4 for preparing a cosmetic.

6. Use of the composition according to any one of claims 1 to 4 for preparing an antibacterial agent.

7. A cosmetic, characterized in that, Containing the composition according to any one of claims 1 to 4.

8. The cosmetic according to claim 7, characterized in that, The cosmetic is shampoo, body wash or hair conditioner.

9. The cosmetic according to claim 7, characterized in that, The cosmetic contains 0.1% to 10% of the composition.

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