Biomolecular aggregate-mediated electrochemical detection method, kit and detection system
By modifying the modified PSP formed by phosphate groups on the polypeptide PS, the specific recognition and dephosphorylation reaction of the target protein is used to form a biomolecular condensed electrochemical indicator, solving the sensitivity and stability problems in homogeneous electrochemical detection methods, and achieving high sensitivity and high stability quantitative detection of the target protein.
CN120334332APending Publication Date: 2025-07-18SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Patent Information
- Application Number
- CN202510351159.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-24
- Publication Date
- 2025-07-18
AI Technical Summary
Technical Problem
The existing homogeneous electrochemical detection methods are insufficient in trace analysis, and electroactive molecules are easily oxidized or degraded, resulting in poor repeatability.
Method used
After the modified phosphate groups are modified on the polypeptide PS, the PSP is used to form a biological molecular condensate to encapsulate an electrochemical indicator, and the high-sensitive quantitative detection of the target protein is achieved through electrochemical signal analysis.
Benefits of technology
It improves the sensitivity and stability of electrochemical detection, and achieves simple and highly sensitive quantitative detection of the target protein, with a detection range of 1ng/mL-100μg/mL, which is easy to operate and good repeatability.
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Figure CN120334332A_ABST
Abstract
The invention discloses a biomolecular condensate mediated electrochemical detection method, a kit and a detection system.The method comprises the steps that a phosphate group is modified on polypeptide PS with the liquid-liquid phase separation characteristic, and modified PSP with the liquid-liquid phase separation characteristic inhibited is obtained; the modified PSP and a to-be-detected sample are subjected to a mixed reaction, an electrochemical indicator is added into a product obtained after the reaction, when target protein exists, the modified PSP specifically recognizes the target protein, phosphate groups can be removed under the action of the target protein to restore the liquid-liquid phase separation characteristic, the liquid-liquid phase separation process is started to form a biomolecule condensate, and the biomolecule condensate is used for detecting the content of the target protein. The biomolecular condensate wraps the electrochemical indicator to change the electrochemical signal, and finally, the target protein in the sample to be detected is identified by analyzing the electrochemical signal. The method is simple, and high-sensitivity quantitative detection of the target protein can be realized.
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