Detection image acquisition device and method

Abstract

In the detection image acquisition device and method, an image sensor module comprises one or two or more than two image sensors; the control module is in electric signal connection with the image sensor module; the control module is in electric signal connection with the light source module; the image sensor module and the light source module are oppositely arranged above or below the sample accommodating module; a sample accommodating cavity in the sample accommodating module is used for bearing a sample; the light source module is used for emitting light rays, and the light rays penetrate through the sample accommodating module and project image information of the visible components onto the image sensor module; the sensor image includes a visible component image. The image acquisition module is used for acquiring images of the antigen-antibody agglomerated microparticles and projecting image information of the agglomerated microparticles onto the image sensor module; the image sensor module obtains a sensor image; the sensor image comprises an agglomerated microparticle image. The microscopic camera shoots the sample accommodating module to obtain a microscopic image; the microscopic image comprises an agglomerated microparticle image.

Description

Technical Field

[0001] This application belongs to the technical field related to the analysis of formed components, and specifically relates to an image acquisition device and method for the analysis of formed components. Background Art

[0002] In the body fluids, excreta or secretions of organisms, such as blood, feces, and urine detection, are routine detection items. Through detection, the morphological and compositional information of various substances contained therein can be obtained, which is helpful for the diagnosis of disease types and courses. The most common fecal detection is to distinguish and identify the components therein with the aid of a microscope, and microscopic examination is carried out using the microscope.

[0003] The applicant has proposed a series of Chinese patents, such as

[0004] 1. CN2020112669290, "Cell Analysis Method and System and Quantitative Method and System";

[0005] 2. CN2020112669182, "Imaging Method and System for Cell Suspension Samples and Kit";

[0006] 3. CN2022104799126, "Fast Focusing Method for Microscopic Image Acquisition Device and Microscopic Image Acquisition Method";

[0007] 4. CN2023105340140, "Fecal Component Antigen-Antibody Particle Detection Method and System and Turbidimetry Dilution Card and Method";

[0008] 5. CN2023113529188, "Fecal Suspension Formed Component Antigen-Antibody Particle Detection Method and Device";

[0009] 6. CN2023103592077, "Quantitative Analysis and Control Method and System for Fecal Formed Components Based on Microscopic Images";

[0010] Use a brand-new technical solution to measure the content of target substances in various body fluids such as blood, urine, and feces, and open up a brand-new technical route to detect the formed components in the suspension.

[0011] Regardless of the image analysis of the formed components in which body fluid, image acquisition is the key. How to efficiently obtain high-quality pictures is the key. For some components that are difficult to observe with the naked eye, how to use technical means to display them so that the image analysis tool can play a role is also a technical problem to be solved. Summary of the Invention

[0012] In this application, the inventor proposes an image acquisition device and method, which combines an image sensor module, a sample accommodation module, a light source module, and a control module in one device to directly acquire images, avoiding the need for complex optical structures and the process of adjusting the focal length or phase in the prior art when obtaining images using a microscope. By adding detection particles to the sample, the surface of the detection particles includes antigens or antibodies; they can bind to the analytes in the sample through antigens or antibodies to form particle conjugates; the particle conjugates aggregate to form aggregated particulate bodies; the size range of the aggregated particulate bodies is comparable to the size of other formed components in the sample. Therefore, the corresponding components can be analyzed and detected by means of image acquisition and analysis of the formed components, expanding the detectable components.

[0013] The technical solution for this application to solve the above technical problems is a detection image acquisition device for the detection of formed components; it includes an image sensor module, a sample accommodation module, a light source module, and a control module; the image sensor module includes one, two, or more than two image sensors; the control module is electrically connected to the image sensor module; the control module is electrically connected to the light source module; the image sensor module and the light source module are relatively arranged above or below the sample accommodation module; the sample accommodation cavity in the sample accommodation module is used to carry the sample; the light source module is used to emit light, and the light passes through the sample accommodation module to project the image information of the formed components onto the image sensor module; the image sensor module obtains a sensor image; the sensor image includes the image of the formed components.

[0014] The pixel size of the image sensor in the above image sensor module is less than 3 microns.

[0015] The above detection image acquisition device further includes a lens module, which includes two or more lenses. The lenses correspond to the image sensors, and the lens module is located between the image sensor module and the sample accommodation module.

[0016] The above image acquisition device is used for antigen-antibody detection; it further includes detection particles; the surface of the detection particles includes antigens or antibodies; the detection particles are used to be added to the sample; the detection particles are used to bind to the analytes in the sample to form particle conjugates; the particle conjugates aggregate to form aggregated particulate bodies; the light source module is used to emit light, and the light passes through the sample accommodation module to project the image information of the aggregated particulate bodies onto the image sensor module; the image sensor module obtains a sensor image; the sensor image includes the image of the aggregated particulate bodies.

[0017] The above formed components include any one or a combination of more than one of: red blood cells, white blood cells, platelets, bacteria, worms, and eggs.

[0018] The technical solution of the present application for solving the above technical problems may also be a detection image acquisition device for acquiring antigen-antibody agglomerated microsome images, and the above image acquisition device is used for antigen-antibody detection; it includes a microscopic camera, a sample accommodation module, a light source module, and a control module; the control module is electrically connected to the microscopic camera; the control module is electrically connected to the light source module; the sample accommodation cavity in the above sample accommodation module is used to carry the sample; it also includes detection microparticles; the surface of the detection microparticles includes antigens or antibodies; the detection microparticles are used to be added to the sample; the detection microparticles are used to bind to the analyte in the sample to form microparticle conjugates; the microparticle conjugates aggregate to form agglomerated microsomes; the microscopic camera photographs the sample accommodation module to obtain a microscopic image; the above microscopic image includes an agglomerated microsome image.

[0019] The above detection image acquisition device includes a distance adjustment device; the distance adjustment device is used to adjust the distance between the microscopic camera and the sample accommodation module; the distance adjustment device is electrically connected to the control module; the magnification of the above microscopic camera is equal to the set value.

[0020] The above detection image acquisition device includes an image calculation module; the image calculation module is used to analyze the above microscopic image or sensor image, identify the above agglomerated microsome image, and identify agglomerated microsomes with a number less than the set value of the number of aggregated microparticles.

[0021] The above set value of the number of aggregated microparticles is greater than 2 and less than 10.

[0022] The above detection image acquisition device includes any one of the following technical features: TA1: The above image calculation module is used to calculate and obtain the total area of the aggregated microparticles, and the total area of the aggregated microparticles does not include agglomerated microsomes with a number less than the set value of the number of aggregated microparticles; TA2: The above image calculation module is used to calculate and obtain the total area of the aggregated microparticles, and the total area of the aggregated microparticles includes agglomerated microsomes with a number greater than the set value of the number of aggregated microparticles.

[0023] The technical solution of the present application for solving the above technical problems may also be a detection image acquisition method, including the following steps: adding detection microparticles to the sample, and the surface of the detection microparticles includes antigens or antibodies; adding the sample to the sample accommodation cavity in the sample accommodation module; the detection microparticles bind to the analyte in the sample to form microparticle conjugates; the microparticle conjugates aggregate to form agglomerated microsomes; light passes through the above sample accommodation module; it also includes any one of the following technical features: TB1: The image information of the formed components is projected onto the above image sensor module; the image sensor module obtains a sensor image; the above sensor image includes a formed component image; TB2: The image information of the agglomerated microsomes is projected onto the above image sensor module; the image sensor module obtains a sensor image; the above sensor image includes an agglomerated microsome image; TB3: The microscopic camera photographs the sample accommodation module to obtain a microscopic image; the above microscopic image includes an agglomerated microsome image.

[0024] The technical effects of the above technical solution include: the image sensor is sheet-shaped, arranged closely to the sample accommodation module, directly obtains images, has a simple optical path, and a simple structure is achieved.

[0025] The technical effects of the above technical solution include: the pixel size of the image sensor is less than 3 microns, which can ensure the accuracy of image acquisition, is suitable for the detection and analysis of formed elements, including images of red blood cells, white blood cells, and various aggregated microparticles with a size in the micron range.

[0026] The technical effects of the above technical solution include: the lens module can converge light to ensure sufficient exposure energy for each image sensor.

[0027] The technical effects of the above technical solution include: based on the images of aggregated microparticles, it can be used for the image analysis and detection of antigen-antibody, bringing immunological detection into a new technical implementation system.

[0028] The technical effects of the above technical solution include: simultaneously performing the detection of formed elements and antigen-antibody.

[0029] The technical effects of the above technical solution include: the images of aggregated microparticles can also be obtained based on a microscopic camera, expanding more acquisition methods.

[0030] The technical effects of the above technical solution include: the distance adjustment device can ensure the clarity of the acquired images.

[0031] The technical effects of the above technical solution include: identifying aggregated microparticles with a number less than the set value of the aggregated particle number, which is used for subsequent identification and calculation to avoid calculation errors introduced by accidentally occurring small numbers of microparticles.

[0032] The technical effects of the above technical solution include: the method for acquiring detection images is simpler and more efficient, and can simultaneously obtain images of formed elements and images of aggregated microparticles. Description of the Drawings

[0033] Figure 1 is a schematic block diagram of the detection image acquisition device Figure 1 ;

[0034] Figure 2 is a schematic block diagram of the detection image acquisition device Figure 2 ;

[0035] Figure 3 is a schematic diagram of the disassembled state of the detection image acquisition device Figure 1 ;

[0036] Figure 4 is a schematic diagram of the disassembled state of the detection image acquisition device Figure 2 ;

[0037] Figure 5 is a schematic partial exploded view of an inspection image acquisition device Figure 1 ;

[0038] Figure 6 is a schematic exploded view of the light source module 1100 in the inspection image acquisition device;

[0039] Figure 7 is a schematic partial exploded view of an inspection image acquisition device Figure 2 ;

[0040] Figure 8 is a top view of the sample accommodation module 1200 in the inspection image acquisition device;

[0041] Figure 9 is a schematic exploded view of an inspection image acquisition device Figure 3 ;

[0042] Figure 10 is a schematic exploded view of an inspection image acquisition device Figure 4 ;

[0043] Figure 11 is a schematic frame of an inspection image acquisition device Figure 3 ;

[0044] Figure 12 is a schematic exploded view of an inspection image acquisition device Figure 5 ;

[0045] Figure 13 is one of the images acquired by the inspection image acquisition device;

[0046] Figure 14 is a partial screenshot of the image acquired by the inspection image acquisition device;

[0047] Figure 15 is a screenshot of various formed components in the image acquired by the inspection image acquisition device. Detailed implementation manners

[0048] The following further details the content of this application in conjunction with each attached drawing. It should be noted that the following is a description of the preferred embodiments of the present invention and does not constitute any limitation to the present invention. The description of the preferred embodiments of the present invention is only for the illustration of the general principles of the present invention. The numbers such as "first", "second", "A", and "B" involved in the present invention are only for the convenience of description and do not represent the order relationship in time or space. The combinations of letters and numbers "TA", "TB", and "H" involved in the present invention are only for the convenience of description, and the specific meanings are determined by the specific words they represent.

[0049] Such as Figures 1 to 2, in an embodiment of an image acquisition device for detection, which is used for formed element detection; it includes an image sensor module, a sample accommodation module, a light source module, and a control module; the control module is electrically connected to the image sensor module; the control module is electrically connected to the light source module. The image sensor module and the light source module are arranged opposite to each other.

[0050] As Figure 1 , in an embodiment of an image acquisition device for detection, the image sensor module and the light source module are arranged opposite to each other; the light source module is arranged above, and the image sensor module is arranged below; the sample accommodation module is arranged between the image sensor module and the light source module.

[0051] As Figure 2 , in an embodiment of an image acquisition device for detection, the others are Figure 1 the same, that is, the light source module is arranged below and the image sensor module is arranged above.

[0052] As Figure 3 , in an embodiment of an image acquisition device for detection, the sample accommodation cavity in the sample accommodation module 1200 is used to carry the sample; the sheet-shaped image sensor 1300 is closely attached to one side of the sample accommodation module 1200, the light source module 1100 emits light, the light passes through the above sample accommodation module 1200, and projects the image information of the formed elements onto the above image sensor module 1300.

[0053] As Figures 3 to 4 , in an embodiment of an image acquisition device for detection, the image sensor module includes one or two or more than two image sensors 1300; Figure 3 The image sensor 1300 in

[0054] As Figures 3 to 5 , in an embodiment of an image acquisition device for detection, the light source module 1100 is located above the image sensor 1300 and the sample accommodation module 1200.

[0055] As Figure 6 , in an embodiment of an image acquisition device for detection, the light source module 1100 includes a sheet-shaped light source main body 1120 and a light homogenizer 1180, and a plurality of LED light sources 1122 are distributed in the center of the sheet-shaped light source main body 1120; the light emitted by the plurality of LED light sources 1122 is output after passing through the light homogenizer 1180. Make the output light more uniform and improve the consistency of the acquired image. The light homogenizer includes frosted glass or multiple layers of frosted glass.

[0056] As Figure 7, In an embodiment of an image acquisition device for detection, the sample accommodation cavity 1230 in the sample accommodation module 1200 is used to hold various samples, and the samples contain formed elements; the samples are in the form of a suspension liquid. The samples include various body fluids, such as blood, urine, diluted fecal samples, body cavity fluids, tissue fluids and other samples to be tested. The formed elements in the image include any one or a combination of red blood cells, white blood cells, platelets, bacteria, worms, and eggs.

[0057] Such as Figure 7 And Figure 8 , In an embodiment of an image acquisition device for detection, the sample accommodation module 1200 includes a sample accommodation cavity 1230 for holding various samples, a sample inlet 1210, and an exhaust port 1220; light passes through the sample accommodation cavity 1230 for imaging projection.

[0058] Figure 7 , In the image sensor 1330 of the above image sensor module 1300, the pixel size of the image sensor 1 is less than 3 microns. It can be used to obtain micron-level image information. The image sensor module 1300 obtains a sensor image; the above sensor image includes a formed element image.

[0059] Such as Figure 13 , Detection particles 7100 can be seen in the figure; the surface of the detection particles includes antigens or antibodies; the detection particles are used to be added to the sample; the detection particles are used to bind to the analyte in the sample to form a particle conjugate; the particle conjugates aggregate to form an aggregated particle body 7200. Figure 14 , In it, the label 7300 is also an aggregated particle body. The light source module emits light to irradiate the sample accommodation module, and projects the image information of the aggregated particle body 7200 or the aggregated particle body 7300 onto the above image sensor module; the image sensor module obtains a sensor image; the above sensor image includes an aggregated particle body image.

[0060] Such as Figure 15 , When the sample is urine, the formed elements in the image include any one or more of red blood cells, white blood cells, bacteria, worms, eggs, and lipid droplets, and also include any one or more of cocci, bacilli, and yeasts. It also includes some cells, such as any one or more of epithelial cells, renal tubular epithelial cells, squamous epithelial cells, and sperm. It also includes various crystals, such as any one or more of magnesium ammonium phosphate crystals, calcium oxalate dihydrate crystals, calcium oxalate monohydrate crystals, and calcium phosphate crystals. It also includes any one or more of hyaline casts, granular casts, cellular casts, and waxy casts.

[0061] Such as Figures 9 to 10, In an embodiment of an image acquisition device for detection, it includes an image sensor module 2300, a sample accommodation module 2200, and a light source module 2100; it further includes a lens module 2500, and the lens module 2500 includes two or more lenses 2550. The above lenses 2550 correspond to the above image sensor 2330, and the lens module 2500 is located between the image sensor module 2300 and the sample accommodation module 2200.

[0062] As Figures 9 to 10 , In an embodiment of an image acquisition device for detection, the sample accommodation module 2200 includes a sample accommodation cavity 2230 for carrying various samples, a sample inlet 2210, and an exhaust port 2220; light passes through the sample accommodation cavity 2230 for imaging projection.

[0063] As Figure 11 , In an embodiment of an image acquisition device for detection, the above image acquisition device is used for antigen-antibody detection; it includes a microscope camera, a sample accommodation module, a light source module, and a control module; the control module is electrically connected to the microscope camera; the control module is electrically connected to the light source module; the sample accommodation cavity in the above sample accommodation module is used for carrying samples; it further includes detection particles; the surface of the detection particles includes antigens or antibodies; the detection particles are used to be added to the samples; the detection particles are used to bind to the substances to be detected in the samples to form particle conjugates; the particle conjugates aggregate to form aggregated particulate bodies; the microscope camera photographs the sample accommodation module to obtain a microscopic image; the above microscopic image includes an image of the aggregated particulate bodies.

[0064] As Figure 12 , In an embodiment of an image acquisition device for detection, the light source module 3100 includes a light source module main body 3120 and a light homogenizer 3180, the sample accommodation module 3200 includes a sample accommodation cavity 3230 for carrying various samples, a sample inlet 3210, and an exhaust port 3220; the microscope camera includes an objective lens 3700, a lens barrel 3600, and a camera 3500. The light source module 3100 is in a planar form, can be combined with the sample accommodation module 3200 into a whole, and can move relative to the microscope camera. It can be combined with the sample accommodation module 3200 into a whole for movement, or can be fixed in position while the microscope camera moves.

[0065] In an embodiment of an image acquisition device for detection not shown in some drawings, it includes a distance adjustment device; the distance adjustment device is used to adjust the distance between the microscope camera and the sample accommodation module; the distance adjustment device is electrically connected to the control module; the magnification of the above microscope camera is equal to a set value. By adjusting the distance adjustment device, a clear focused microscopic image can be obtained.

[0066] As Figures 1 to 2 , and Figure 12, in an embodiment of a detection image acquisition device, it includes an image calculation module; the image calculation module is used to analyze the above microscopic image or sensor image, identify the above agglomerated particulate images, and identify agglomerated particulate bodies with a number less than the set value of the number of aggregated particulates. The set value of the number of aggregated particulates is greater than 2 and less than 10.

[0067] Figure 13 , in an embodiment of a detection image acquisition device, the above image calculation module is used to calculate the total area of the aggregated particulates, and the total area of the aggregated particulates does not include agglomerated particulate bodies with a number less than the set value of the number of aggregated particulates; if the set value of the number of aggregated particulates is 3, then Figure 13 the 7200 agglomerated particulate bodies therein do not participate in the calculation of the total area of the aggregated particulates. The total area of the aggregated particulates includes agglomerated particulate bodies with a number greater than the set value of the number of aggregated particulates. Figure 14 The 7200 agglomerated particulate bodies therein participate in the calculation of the total area of the aggregated particulates.

[0068] In an embodiment of a detection image acquisition method not shown in some of the drawings, it includes the following steps: adding detection particulates to a sample, where the surface of the detection particulates includes an antigen or an antibody; adding the sample to a sample accommodation cavity in a sample accommodation module; the detection particulates bind to the analyte in the sample to form particulate conjugates; the particulate conjugates aggregate to form agglomerated particulate bodies; light passes through the above sample accommodation module; the image information of the formed components is projected onto the above image sensor module; the image sensor module obtains a sensor image; the above sensor image includes the image of the formed components.

[0069] In an embodiment of a detection image acquisition method not shown in some of the drawings, it includes the following steps: adding detection particulates to a sample, where the surface of the detection particulates includes an antigen or an antibody; adding the sample to a sample accommodation cavity in a sample accommodation module; the detection particulates bind to the analyte in the sample to form particulate conjugates; the particulate conjugates aggregate to form agglomerated particulate bodies; light passes through the above sample accommodation module; projecting the image information of the agglomerated particulate bodies onto the above image sensor module; the image sensor module obtains a sensor image; the above sensor image includes the image of the agglomerated particulate bodies;

[0070] In an embodiment of a detection image acquisition method not shown in some of the drawings, it includes the following steps: adding detection particulates to a sample, where the surface of the detection particulates includes an antigen or an antibody; adding the sample to a sample accommodation cavity in a sample accommodation module; the detection particulates bind to the analyte in the sample to form particulate conjugates; the particulate conjugates aggregate to form agglomerated particulate bodies; light passes through the above sample accommodation module; a microscope camera photographs the sample accommodation module to obtain a microscopic image; the above microscopic image includes the image of the agglomerated particulate bodies.

[0071] Although the present invention has been described and illustrated with reference to preferred embodiments and several alternatives, the invention is not limited to the specific description herein. Other additional alternatives or equivalent components may also be used to practice the present invention.

Claims

1. A detection image acquisition device for formed element detection; characterized in that, it includes an image sensor module, a sample accommodation module, a light source module, and a control module; the image sensor module includes one, two, or more than two image sensors; the control module is electrically connected to the image sensor module; the control module is electrically connected to the light source module; the image sensor module and the light source module are relatively arranged above or below the sample accommodation module; the sample accommodation cavity in the sample accommodation module is used to carry a sample; the light source module is used to emit light, and the light passes through the sample accommodation module to project the image information of the formed elements onto the image sensor module; the image sensor module obtains a sensor image; the sensor image includes the formed element image.

2. The detection image acquisition device according to claim 1, characterized in that, the pixel size of the image sensor in the image sensor module is less than 3 microns.

3. The detection image acquisition device according to claim 1, characterized in that, it further includes a lens module, the lens module includes two or more than two lenses, the lenses correspond to the image sensors, and the lens module is located between the image sensor module and the sample accommodation module.

4. The detection image acquisition device according to claim 1, characterized in that, the image acquisition device is used for antigen-antibody detection; it further includes detection microparticles; the surface of the detection microparticles includes an antigen or an antibody; the detection microparticles are used to be added to the sample; the detection microparticles are used to bind to the analyte in the sample to form a microparticle conjugate; the microparticle conjugates aggregate to form agglomerated microparticle bodies; the light source module is used to emit light, and the light passes through the sample accommodation module to project the image information of the agglomerated microparticle bodies onto the image sensor module; the image sensor module obtains a sensor image; the sensor image includes the agglomerated microparticle body image.

5. The detection image acquisition device according to claim 1, characterized in that, the formed elements include any one or a combination of more than one of: red blood cells, white blood cells, platelets, bacteria, worms, and eggs.

6. A detection image acquisition device for acquiring antigen-antibody agglomerated microsome images, characterized in that, The image acquisition device is used for antigen-antibody detection; it includes a microscope camera, a sample accommodation module, a light source module, and a control module; the control module is electrically connected to the microscope camera; the control module is electrically connected to the light source module; the sample accommodation cavity in the sample accommodation module is used to carry a sample; it further includes detection microparticles; the surface of the detection microparticles includes an antigen or an antibody; the detection microparticles are used to be added to the sample; the detection microparticles are used to bind to the analyte in the sample to form a microparticle conjugate; the microparticle conjugates aggregate to form agglomerated microparticle bodies; the microscope camera photographs the sample accommodation module to obtain a microscopic image; the microscopic image includes the agglomerated microparticle body image.

7. The detection image acquisition device according to claim 6, characterized in that, it includes a distance adjustment device; the distance adjustment device is used to adjust the distance between the microscope camera and the sample accommodation module; the distance adjustment device is electrically connected to the control module; the magnification of the microscope camera is equal to a set value.

8. The detection image acquisition device according to any one of claims 1 to 7, characterized in that, It includes an image calculation module; the image calculation module is used to analyze the microscopic image or sensor image, identify the agglomerated particulate image, and identify the agglomerated particulate bodies with a number less than the set value of the number of aggregated particulates.

9. The formed component detection image acquisition device according to claim 8, wherein the set value of the number of aggregated particulates is greater than 2 and less than 10.

10. The detection image acquisition device according to claim 8, wherein it includes any one of the following technical features: TA1: The image calculation module is used to calculate the total area of the aggregated particulates, and the total area of the aggregated particulates does not include the agglomerated particulate bodies with a number less than the set value of the number of aggregated particulates; TA2: The image calculation module is used to calculate the total area of the aggregated particulates, and the total area of the aggregated particulates includes the agglomerated particulate bodies with a number greater than the set value of the number of aggregated particulates.

11. A method for acquiring a detection image, characterized in that, It includes the following steps: Adding detection particulates to the sample, and the surface of the detection particulates includes antigens or antibodies; Adding the sample to the sample accommodation cavity in the sample accommodation module; The detection particulates and the analyte in the sample bind to form particulate conjugates; The particulate conjugates aggregate to form agglomerated particulate bodies; Light passes through the sample accommodation module; It also includes any one of the following technical features: TB1: The image information of the formed component is projected onto the image sensor module; the image sensor module obtains a sensor image; the sensor image includes the image of the formed component; TB2: The image information of the agglomerated particulate bodies is projected onto the image sensor module; the image sensor module obtains a sensor image; the sensor image includes the image of the agglomerated particulate bodies; TB3: The microscopic camera photographs the sample accommodation module to obtain a microscopic image; the microscopic image includes the image of the agglomerated particulate bodies.

Images