Insemination device for artificial insemination of Chinese and western bees

Abstract

The invention relates to the technical field of insemination devices, and discloses an insemination device used for artificial insemination of Chinese and western bees, the insemination device comprises a spiral injector body, a cooling mechanism and a heating mechanism, the inside of the spiral injector body is fixedly connected with a storage tube, and the outer side of the spiral injector body is fixedly connected with a sleeve; a temperature sensor is arranged in the sleeve, the storage tube is located in the sleeve, a switching mechanism is arranged at the bottom of the sleeve, a needle tip is installed at the bottom of the switching mechanism, the cooling mechanism comprises a liquid carbon dioxide storage tank, and the liquid carbon dioxide storage tank is located outside the spiral injector body. And the outer side of the liquid carbon dioxide storage tank is fixedly connected with a conveying pipe. The temperature rising or cooling mechanism is flexibly started according to the room temperature, the temperature in the storage pipe is continuously adjusted to be kept in a constant-temperature state suitable for sperm preservation, a good preservation environment is provided for sperms, and therefore the insemination effect is improved.

Description

Technical Field

[0001] The invention relates to the technical field of insemination devices, in particular to an insemination device used for artificial insemination of Chinese and Western honey bees. Background Art

[0002] During the artificial insemination of the queen bee, the first thing to do is to collect semen from sexually mature drones, which can be as little as tens of microliters or as much as hundreds of microliters, and then carry out the insemination process. In this way, it is necessary to prepare multiple sets of syringes in advance. The drone semen collection devices currently used use 100l medical injectors or 5ml syringes for semen collection and insemination. The performance of these two syringes can meet the needs of drone semen collection and queen bee insemination, and have been used for many years at home and abroad.

[0003] However, after many actual operations, it was found that both the medical injector and the syringe are alternatives with shortcomings. They are more suitable for teaching or the need of insemination of a small number of queen bees. For units engaged in professional bee scientific research or with the need of artificial insemination of large quantities of queen bees, especially when the amount of drone semen collected on the same day is large and the time is long, or the insemination process cannot be completed in a short time, the semen will be affected by the external temperature after leaving the bee body, thus changing the sperm quality.

[0004] With the progress of the times and the rapid development of beekeeping, the demand for bee species selection and breeding and the preservation of fine varieties is also on the rise. Therefore, only by improving the quality and technology of artificial insemination of queen bees can the pace of modernization and scientific development be met. During the artificial insemination of queen bees, when the temperature difference between the outside and the indoor temperature is large, the collection of spare drone semen needs to withstand the impact of high and low temperatures for a long time, which will cause great damage to sperm. At this time, it is injected into the body of the queen bee, which also has a great impact on the quality of the queen bee after insemination. The queen bee's egg-laying ability is reduced, the area of the brood spleen is reduced, it is impossible to reproduce into a strong group, and the collection ability is reduced. In addition, for the honey bee seed preservation, it is easy to have the phenomenon of decreased sperm motility, gene loss, species degeneration and accelerated inbreeding, which seriously affects the development of the honey bee industry and the honey bee artificial insemination technology. Therefore, the present invention provides a kind of insemination device used for artificial insemination of Chinese and Western honey bees to solve the shortcomings in the prior art. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides an insemination device for artificial insemination of Chinese and Western honey bees, which solves the problem that the semen in the honey bee insemination device in the prior art is easily affected by the external temperature, resulting in poor insemination effect.

[0006] To achieve the above object, the present invention is realized through the following technical solutions: An insemination device for artificial insemination of Chinese and Western honeybees includes a spiral syringe body, a cooling mechanism, and a heating mechanism. A storage tube is fixedly connected inside the spiral syringe body. A sleeve is fixedly connected to the outside of the spiral syringe body. A temperature sensor is arranged inside the sleeve. The storage tube is located inside the sleeve. A transfer mechanism is arranged at the bottom of the sleeve. A needle tip is installed at the bottom of the transfer mechanism. The cooling mechanism includes a liquid carbon dioxide storage tank. The liquid carbon dioxide storage tank is located outside the spiral syringe body. A delivery pipe is fixedly connected to the outside of the liquid carbon dioxide storage tank. One end of the delivery pipe is fixedly connected to the outside of the sleeve. A plurality of exhaust holes are opened on the outside of the sleeve, and a one-way valve is arranged inside the exhaust holes.

[0007] Preferably, the heating mechanism is arranged inside the sleeve. The heating mechanism includes a spiral heating coil. The spiral heating coil is sleeved on the outer wall of the storage tube. One end of the spiral heating coil is electrically connected to a wire. One end of the wire is electrically connected to a heating switch.

[0008] Preferably, the transfer mechanism includes an assembly component and a sealing component. The assembly component includes a combined block. Installation grooves are opened at the top and bottom of the combined block. The bottom end of the storage tube is clamped inside the top installation groove. The top end of the needle tip is clamped inside the bottom installation groove.

[0009] Preferably, a sealing ring is sleeved on the outer periphery of the storage tube. The bottom of the sealing ring fits with the top of the combined block. The top of the sealing ring fits with the bottom of the sleeve.

[0010] Preferably, two connecting blocks are fixedly connected to the outside of the combined block. A limiting groove is opened on the outside of the connecting block. Two limiting blocks are fixedly connected to the outside of the sleeve. The outside of the limiting block is clamped inside the limiting groove.

[0011] Preferably, the sealing component includes a turntable. The turntable is rotatably connected inside the combined block. A communication hole is opened inside the turntable. A hole is opened between the inner walls of the two installation grooves. The communication hole is communicated with the hole.

[0012] Preferably, a threaded rod is arranged inside the spiral syringe body. One end of the threaded rod is fixedly connected to a handle. One end of the handle is fixedly connected to a piston. The outside of the piston is slidably connected to the inner wall of the storage tube.

[0013] A heat preservation method for an insemination device used for artificial insemination of Chinese and Western honeybees, which is applied to the above-mentioned insemination device for artificial insemination of Chinese and Western honeybees, includes the following steps:

[0014] When the room temperature is greater than 14°C, first, the flow switch is used to make the liquid carbon dioxide inside the liquid carbon dioxide storage tank enter the inside of the sleeve along the delivery pipe to cool the storage pipe, making the temperature approach 14°C. When the temperature is lower than 14°C, the heating switch is activated to make the spiral electric heating coil heat up and control the temperature to 14°C.

[0015] When the room temperature is less than 14°C, first, the heating switch is activated to make the spiral electric heating coil heat the inside of the storage pipe. When the temperature exceeds 14°C, the temperature sensor will open the valve in the delivery pipe to let the liquid carbon dioxide cool the storage pipe, making the temperature approach 14°C.

[0016] The present invention provides an insemination device for artificial insemination of Chinese and Western honeybees. It has the following beneficial effects:

[0017] 1. The present invention can flexibly activate the heating or cooling mechanism according to the room temperature. When the room temperature is low, it heats up first. It is heated by the spiral electric heating coil and regulated by the temperature sensor to avoid the influence of too low temperature on sperm motility. When the room temperature is high, it cools down first. It uses the vaporization heat absorption of liquid carbon dioxide to cool down, and the one-way valve exhausts to ensure stable pressure. Through continuous adjustment, the temperature inside the storage pipe is maintained at a constant temperature suitable for sperm preservation, providing a good preservation environment for sperm, thereby improving the insemination effect.

[0018] 2. When inseminating, the turntable is rotated to connect the storage pipe and the needle tip for operation. When it is closed, the constant temperature inside the pipe is maintained. After the operation is completed, the combined block is rotated to separate the limiting structure, and the combined block and the needle tip can be easily removed. This detachable design is convenient for thorough cleaning of each part of the device, effectively preventing semen residue, ensuring that subsequent use is not affected, and improving the practicality and hygiene level of the syringe. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a perspective view of the present invention;

[0020] Figure 2 is a schematic structural diagram of the sleeve of the present invention;

[0021] Figure 3 is a schematic internal structure diagram of the sleeve of the present invention;

[0022] Figure 4 is a schematic structural diagram of the needle tip of the present invention;

[0023] Figure 5 is Figure 4 an enlarged view of part A in

[0024] Among them, 1. Spiral syringe body; 2. Liquid carbon dioxide storage tank; 3. Delivery pipe; 4. Flow switch; 5. Storage pipe; 6. Sleeve; 7. Exhaust hole; 8. Needle tip; 9. Spiral electric heating coil; 10. Electric wire; 11. Heating switch; 12. Threaded rod; 13. Handle; 14. Combined block; 15. Installation groove; 16. Turntable; 17. Communication hole; 18. Sealing ring; 19. Connection block; 20. Limiting groove; 21. Limiting block. Detailed implementation manner

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0026] Please refer to the attached Figure 1 - attached Figure 5, an embodiment of the present invention provides an insemination device for artificial insemination of Chinese and Western honeybees, which includes a spiral syringe body 1, a cooling mechanism and a heating mechanism. The spiral syringe body 1 is the basic framework of the whole device, and a storage tube 5 is fixedly connected inside it for storing drone semen. A threaded rod 12 is arranged inside the spiral syringe body 1. One end of the threaded rod 12 is fixedly connected to a handle 13, and one end of the handle 13 is fixedly connected to a piston. The outer side of the piston is slidably connected to the inner wall of the storage tube 5. A sleeve 6 is fixedly connected to the outer side of the spiral syringe body 1. A temperature sensor is arranged inside the sleeve 6, which can monitor the temperature inside the sleeve 6 in real time. The storage tube 5 is located inside the sleeve 6, and the sleeve 6 provides a relatively stable temperature environment for the storage tube 5. A transfer mechanism is arranged at the bottom of the sleeve 6, and a needle tip 8 is installed at the bottom of the transfer mechanism for injecting the semen in the storage tube 5 into the queen bee body. The cooling mechanism includes a liquid carbon dioxide storage tank 2, which is located outside the spiral syringe body 1 for storing liquid carbon dioxide. A delivery pipe 3 is fixedly connected to the outer side of the liquid carbon dioxide storage tank 2. One end of the delivery pipe 3 is fixedly connected to the outer side of the sleeve 6. A plurality of exhaust holes 7 are opened on the outer side of the sleeve 6, and a one-way valve is arranged inside the exhaust holes 7. When the cooling mechanism is started, the valve inside the delivery pipe 3 is opened by the cooling inductor, so that carbon dioxide gas enters the inner space of the sleeve 6 through the delivery pipe 3. Since the vaporization of liquid carbon dioxide will absorb a large amount of heat, the temperature inside the sleeve 6 is reduced. At the same time, the hot air inside the sleeve 6 and the carbon dioxide gas flow are discharged through the exhaust holes 7. The one-way valve ensures that the gas can only be discharged outward, preventing external air from entering. In this way, it can not only ensure that the pressure inside the sleeve 6 will not be unbalanced, but also be conducive to rapid cooling. Since the cooling speed of liquid carbon dioxide gas is relatively slow compared with liquid nitrogen, it takes a long time to output gas to achieve the cooling effect, so the cooling process is relatively long, but it can keep the constant temperature state of the storage tube 5 for a long time. The heating mechanism is arranged inside the sleeve 6, including a spiral heating coil 9, an electric wire 10 and a heating switch 11. The spiral heating coil 9 is sleeved on the outer wall of the storage tube 5. When heating is required, the spiral heating coil 9 is started through the heating switch 11. The spiral heating coil 9 generates heat when powered on to heat the storage tube 5. The electric wire 10, as a conductive component connecting the spiral heating coil 9 and the heating switch 11, transmits electrical energy to the spiral heating coil 9.

[0027] The transfer mechanism includes an assembly component and a sealing component. The assembly component includes a combined block 14. Installation grooves 15 are provided at both the top and bottom of the combined block 14. The bottom end of the storage tube 5 is snapped inside the top installation groove 15, and the top end of the needle tip 8 is snapped inside the bottom installation groove 15, realizing the preliminary connection of the storage tube 5 and the needle tip 8. A sealing ring 18 is sleeved on the outer periphery of the storage tube 5. The bottom of the sealing ring 18 is attached to the top of the combined block 14, and the top of the sealing ring 18 is attached to the bottom of the sleeve 6, playing a sealing role to prevent semen leakage and the entry of external air. Two connecting blocks 19 are fixedly connected to the outside of the combined block 14. A limiting groove 20 is provided on the outside of the connecting block 19. Two limiting blocks 21 are fixedly connected to the outside of the sleeve 6. The outside of the limiting block 21 is snapped inside the limiting groove 20, further strengthening the connection stability between the combined block 14 and the sleeve 6. The sealing component includes a turntable 16. The turntable 16 is rotatably connected inside the combined block 14. A communication hole 17 is provided inside the turntable 16. A hole is provided between the inner walls of the two installation grooves 15. The communication hole 17 is communicated with the hole. Rotating the turntable 16 can make the communication hole 17 communicate with or close the storage tube 5 and the needle tip 8. When it is communicated, insemination operations can be carried out, and when it is closed, the temperature inside the storage tube 5 can be kept constant.

[0028] Please refer to the attached Figure 1 - attached Figure 5 The embodiment of the present invention also provides a heat preservation method for an insemination device used in the artificial insemination of Chinese and Western honeybees, including the following steps: When the room temperature is greater than 14°C, first, through the flow switch 4, the liquid carbon dioxide inside the liquid carbon dioxide storage tank 2 enters the inside of the sleeve 6 along the delivery pipe 3 to cool the storage tube 5, making the temperature approach 14°C. When the temperature is lower than 14°C, the heating switch 11 is activated to make the spiral heating coil 9 heat, and the temperature is controlled to 14°C; when the room temperature is less than 14°C, first, the heating switch 11 is activated to let the spiral heating coil 9 heat the inside of the storage tube 5. When the temperature exceeds 14°C, the temperature sensor will open the valve in the delivery pipe 3 to let the liquid carbon dioxide cool the storage tube 5, making the temperature approach 14°C.

[0029] Specifically, when the room temperature is lower than 14°C, the environmental temperature is relatively low at this time, which is not conducive to the preservation of drone semen. Therefore, it is necessary to first increase the temperature inside the storage tube 5 through the temperature increase mechanism. The specific operation is to press the heating switch 11 to activate the spiral electric heating coil 9. The spiral electric heating coil 9 is sleeved on the outer wall of the storage tube 5 and will generate heat after being powered on, starting to heat the storage tube 5. During this process, the temperature sensor plays a key role. It can monitor the temperature inside the sleeve 6 in real time, so as to ensure that the temperature can be controlled between 12 and 16°C. When the temperature is lower than 12°C, it indicates that the current temperature is too low and is not conducive to the survival of drone sperm. At this time, the heating function will be automatically activated to continuously supply heat to the storage tube 5. When the temperature is heated to 14°C, this is the optimal temperature for short-term preservation of drone sperm. At this temperature, it can not only reduce the large amount of activities of sperm, reduce energy consumption, but also effectively preserve the vitality of sperm. Therefore, the heating function will stop at this time to avoid damage to sperm caused by too high temperature. As time goes by, the temperature inside the storage tube 5 will gradually increase. When the temperature gradually rises to 16°C, after the temperature sensor detects this situation, it will trigger the temperature decrease mechanism to start automatically. The temperature decrease sensor will open the valve inside the delivery pipe 3, so that the carbon dioxide gas in the liquid carbon dioxide storage tank 2 passes through the delivery pipe 3 and enters the internal space of the sleeve 6. The liquid carbon dioxide will absorb a large amount of heat during the vaporization process, thereby reducing the temperature inside the sleeve 6. At the same time, the hot air and carbon dioxide gas flow inside the sleeve 6 will be discharged through the exhaust hole 7. The one-way valve inside the exhaust hole 7 ensures that the gas can only be discharged outward, preventing external air from entering. This can not only ensure that the pressure inside the sleeve 6 will not be unbalanced and prevent the semen inside the storage tube 5 from being affected by the pressure change, but also is conducive to rapid cooling. However, since the cooling speed of the liquid carbon dioxide gas is not as fast as that of liquid nitrogen, it needs to output gas for a long time to achieve the ideal cooling effect. Therefore, the cooling time is relatively long. But this slow cooling process also has advantages. It can maintain the constant temperature state of the storage tube 5 for a long time, which is more conducive to the drone semen being in the optimal temperature for a long time. When the temperature drops to 14°C, the liquid carbon dioxide control valve will reduce the flow rate, but there will still be a small amount of gas continuing to be output. This small amount of gas output can maintain the temperature stability inside the tube, so that the temperature inside the tube will not drop rapidly and is basically in a long-term constant temperature state until the temperature inside the tube is lower than 14°C, the valve in the delivery pipe 3 will be completely closed. After the cooling process is completely stopped, due to the heat preservation performance of the sleeve 6 and the storage tube 5 itself, the temperature of the storage tube 5 will still maintain a stable state for a long time. Only after being strongly interfered by the external temperature will there be rapid high and low temperature changes. When the temperature is lower than 12°C again, the temperature increase mechanism will be started again, and so on in a cycle until the work of collecting drone semen and inseminating the queen bee is completed. When the room temperature is higher than 14°C, the environmental temperature is relatively high at this time, which is also not conducive to the preservation of drone semen. Therefore, the temperature decrease mechanism is first started. After the temperature decrease mechanism is started, the temperature is adjusted according to the above temperature decrease process when the room temperature is higher than 16°C.The temperature is monitored in real time through a temperature sensor, and through the coordinated operation of the heating and cooling mechanisms, the temperature in the storage tube 5 is maintained between 12 - 16 °C, providing a suitable preservation environment for drone semen. During the insemination operation, the turntable 16 is rotated so that the communication hole 17 inside the turntable 16 is connected to the storage tube 5 and the needle tip 8. In this way, the drone semen in the storage tube 5 can be smoothly injected into the queen bee's body through the communication hole 17 and the needle tip 8 to complete the insemination operation. When the storage tube 5 and the needle tip 8 are in a closed state, it can effectively reduce the influence of the external environment on the internal temperature of the storage tube 5, ensuring that the internal temperature of the storage tube 5 remains constant and providing stable preservation conditions for drone semen. After the insemination operation is completed, the device needs to be cleaned to avoid semen residue on the inner wall, which may affect the next use. The specific operation is to rotate the combined block 14 so that the limiting block 21 on the outer side of the sleeve 6 is separated from the limiting groove 20 on the connecting block 19 on the outer side of the combined block 14. In this way, the combined block 14 can be easily removed, and then the needle tip 8 is withdrawn from the installation groove 15. This detachable design makes the cleaning of the device more thorough, can effectively avoid semen residue on the inner wall, and ensures the hygiene of the device and the effect of the next use.,

[0030] Working principle: First, the cooling mechanism and the heating mechanism can be started successively according to the room temperature. When the room temperature is lower than 14°C, the spiral electric heating coil 9 is first started through the heating switch 11 to heat the storage tube 5. The temperature can be controlled between 12°C and 16°C. When the temperature is lower than 12°C, the heating function is started and stops heating when the temperature reaches 14°C. At this time, the temperature inside the storage tube 5 increases and gradually rises to 16°C. The temperature sensor will cause the cooling mechanism to start automatically. The internal valve of the delivery pipe 3 is opened by the cooling inductor, so that carbon dioxide gas enters the internal space of the sleeve 6 through the delivery pipe 3, and the hot air and carbon dioxide gas flow in the sleeve 6 are discharged through the exhaust hole 7. In this way, the pressure inside the sleeve 6 will not be unbalanced, which is also conducive to rapid cooling. Since the liquid carbon dioxide gas does not cool down as fast as liquid nitrogen, it takes a long time to output gas to achieve the cooling effect, so the cooling time also needs to be longer. In this way, the constant temperature state of the storage tube 5 can be maintained for a long time, which is more conducive to the drone semen being in the best temperature for a long time. Experiments have proved that after the drone sperm leave the bee body, it is best stored at a temperature of about 14°C in the short term, which can reduce the large amount of sperm activity and preserve the sperm vitality. When the temperature drops to 14°C, the flow rate of the liquid carbon dioxide control valve will be reduced, but a small amount of gas will still be output. At this time, the temperature inside the tube will not drop rapidly and is basically in a long-term constant temperature state until the temperature inside the tube is lower than 14°C, and the valve in the delivery pipe 3 will be completely closed. After the cooling process is completely stopped, the temperature of the storage tube 5 will still maintain a stable state for a long time. Only after being strongly interfered by the external temperature will there be rapid high and low temperature changes. When the temperature is lower than 12°C, the heating mechanism is started again and cycles in turn until the work of collecting drone semen and inseminating the queen bee is completed. When the room temperature is higher than 14°C, the cooling mechanism is started first; in addition, rotating the turntable 16 can connect the communication hole 17 with the storage tube 5 and the needle tip 8 for insemination operation. When the storage tube 5 and the needle tip 8 are closed, the temperature inside the storage tube 5 can be kept constant. After the insemination operation is completed, the combined block 14 can be rotated to separate the limit block 21 from the limit groove 20, and then the combined block 14 can be easily removed, and the needle tip 8 can be pulled out from the installation groove 15. In this way, the device can be cleaned more thoroughly and the semen residue on the inner wall can be avoided.

[0031] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An insemination device for artificial insemination of Chinese and Western honeybees, comprising a spiral syringe body (1), a cooling mechanism and a heating mechanism, characterized in that, Inside the spiral syringe body (1), a storage tube (5) is fixedly connected. On the outside of the spiral syringe body (1), a sleeve (6) is fixedly connected. A temperature sensor is arranged inside the sleeve (6). The storage tube (5) is located inside the sleeve (6). At the bottom of the sleeve (6), a transfer mechanism is provided. At the bottom of the transfer mechanism, a needle tip (8) is installed. The cooling mechanism includes a liquid carbon dioxide storage tank (2). The liquid carbon dioxide storage tank (2) is located outside the spiral syringe body (1). On the outside of the liquid carbon dioxide storage tank (2), a delivery pipe (3) is fixedly connected. One end of the delivery pipe (3) is fixedly connected to the outside of the sleeve (6). A plurality of exhaust holes (7) are opened on the outside of the sleeve (6), and a one-way valve is arranged inside the exhaust holes (7).

2. The insemination device for artificial insemination of Chinese and Western honeybees according to claim 1, characterized in that, The heating mechanism is arranged inside the sleeve (6). The heating mechanism includes a spiral heating coil (9). The spiral heating coil (9) is sleeved on the outer wall of the storage tube (5). One end of the spiral heating coil (9) is electrically connected to a wire (10). One end of the wire (10) is electrically connected to a heating switch (11).

3. The insemination device for artificial insemination of Chinese and Western honeybees according to claim 1, characterized in that, The transfer mechanism includes an assembly component and a sealing component. The assembly component includes a combined block (14). Installation grooves (15) are opened at the top and bottom of the combined block (14). The bottom end of the storage tube (5) is clamped inside the top installation groove (15). The top end of the needle tip (8) is clamped inside the bottom installation groove (15).

4. The insemination device for artificial insemination of Chinese bees and Western bees according to claim 3, characterized in that, A sealing ring (18) is sleeved on the outer circumference of the storage tube (5). The bottom of the sealing ring (18) is attached to the top of the combined block (14). The top of the sealing ring (18) is attached to the bottom of the sleeve (6).

5. The insemination device for artificial insemination of Chinese and Western honeybees according to claim 4, characterized in that, Two connecting blocks (19) are fixedly connected to the outside of the combined block (14). A limiting groove (20) is opened on the outside of the connecting block (19). Two limiting blocks (21) are fixedly connected to the outside of the sleeve (6). The outside of the limiting block (21) is clamped inside the limiting groove (20).

6. The insemination device for artificial insemination of Chinese bees and Western bees according to claim 5, characterized in that, The sealing component includes a turntable (16). The turntable (16) is rotatably connected inside the combined block (14). A communication hole (17) is opened inside the turntable (16). A hole is opened between the inner walls of the two installation grooves (15). The communication hole (17) is communicated with the hole.

7. The insemination device for artificial insemination of Chinese and Western honeybees according to claim 1, characterized in that, A threaded rod (12) is arranged inside the spiral syringe body (1). One end of the threaded rod (12) is fixedly connected to a handle (13). One end of the handle (13) is fixedly connected to a piston, and the outside of the piston is slidably connected to the inner wall of the storage tube (5).

8. A heat preservation method for an insemination device used in artificial insemination of Chinese and Western honeybees, which is applied to the insemination device used in artificial insemination of Chinese and Western honeybees according to any one of claims 1-7, characterized in that, Including the following steps: When the room temperature is greater than 14 °C, first, through the flow switch (4), the liquid carbon dioxide inside the liquid carbon dioxide storage tank (2) enters the inside of the sleeve (6) along the delivery pipe (3) to cool the storage tube (5) so that the temperature approaches 14 °C. When the temperature is lower than 14 °C, the heating switch (11) is started to make the spiral heating coil (9) heat up and control the temperature to 14 °C; When the room temperature is less than 14 °C, first turn on the heating switch (11) to let the spiral electric heating coil (9) heat the inside of the storage tube (5). When the temperature exceeds 14 °C, the temperature sensor will open the valve in the delivery pipe (3) to let the liquid carbon dioxide cool the storage tube (5) so that the temperature approaches 14 °C.

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