Corn growth regulator and application thereof in promoting corn growth and improving salt tolerance of corn

Abstract

The invention provides a corn growth regulator and application thereof in promoting corn growth and improving salt tolerance of corn, and belongs to the technical field of plant growth regulation.The corn growth regulator provided by the invention takes 3-(2-aminoethyl) indole as an active ingredient, and after the corn growth regulator provided by the invention is mixed and applied to soil for treatment, the salt tolerance of the corn can be improved, so that the corn growth regulator has good application prospects. The plant height, the biomass and the IAA content in leaves of the corn land are obviously increased; in a salt stress environment, the biomass of corn plants in spraying treatment of the 3-(2-aminoethyl) indole is obviously higher than that of a control group. The corn growth regulator provided by the invention takes 3-(2-aminoethyl) indole as an active ingredient, is simple, convenient, green and economical in use method, can be applied to agricultural production and related research fields, and ensures corn crop production in a salinization environment.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulation, and particularly relates to a corn growth regulator and its application in promoting corn growth and improving the salt tolerance of corn. Background Art

[0002] As an important global food, feed, and industrial raw material crop, corn plays a crucial role in ensuring food security, promoting the development of animal husbandry, and supporting industrial production. However, the growth and yield of corn are often restricted by various factors, among which abiotic stress, especially salt stress, has become one of the important environmental factors limiting the expansion of corn planting area and the increase in yield.

[0003] On the other hand, during the growth process of corn, sufficient and appropriate growth regulation is extremely crucial for aspects such as plant morphology construction, nutrient accumulation, and reproductive growth. The use of plant growth regulators can improve crop growth and the salt tolerance level under saline or salt stress environments. It is the most effective and direct method for strengthening seedlings, and can break through environmental limitations to achieve direct agricultural utilization, with both ecological and economic benefits. Currently, the known types of plant growth regulators are limited. Therefore, developing a growth regulator with dual functions of promoting growth and resisting salt has great practical significance for promoting the sustainable development of the corn industry against the backdrop of the increasing salinized arable land. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a corn growth regulator and its application in promoting corn growth and improving the salt tolerance of corn; the corn growth regulator provided by the present invention uses 3-(2-aminoethyl)indole as the active ingredient. After being treated with the corn growth regulator provided by the present invention, the above-ground plant height, biomass, and IAA content in the leaves of corn increase significantly; under salt stress conditions, the biomass of corn plants in the 3-(2-aminoethyl)indole spraying treatment is significantly higher than that of the control group.

[0005] The present invention provides a corn growth regulator, which includes 3-(2-aminoethyl)indole.

[0006] Preferably, the 3-(2-aminoethyl)indole is applied in the form of a 3-(2-aminoethyl)indole solution.

[0007] Preferably, the concentration of the 3-(2-aminoethyl)indole solution is 0.01 - 1 mg / mL.

[0008] The present invention provides the application of the described corn growth regulator in promoting the growth of corn seedlings.

[0009] Preferably, the corn growth regulator is used by mixing with soil before planting corn; the dosage of 3-(2-aminoethyl)indole is 0.001 - 0.030 mg / kg of soil.

[0010] Preferably, promoting the growth of corn seedlings includes increasing the biomass of corn seedlings, increasing the plant height of corn seedlings and / or increasing the auxin content in corn leaves.

[0011] The present invention provides the application of the described corn growth regulator in improving the salt tolerance of corn.

[0012] Preferably, the using method of the corn growth regulator is to spray the above-ground part of the corn plant, the corn plant is a corn plant planted for 20 - 40 days, the spraying time is from 9:00 to 11:00 in the morning; the spraying is carried out continuously for 5 - 10 days.

[0013] Preferably, the concentration of the corn growth regulator is 10 - 30 mg / L.

[0014] Compared with the prior art, the present invention has the following beneficial effects: The corn growth regulator provided by the present invention uses 3-(2-aminoethyl)indole as the active ingredient. After treating the soil with the corn growth regulator provided by the present invention, the above-ground plant height, biomass and IAA content in the leaves of the planted corn are significantly increased, and the promoting effect on corn can be achieved with a low-concentration treatment.

[0015] To clarify whether 3-(2-aminoethyl)indole improves the salt tolerance of corn, the present invention conducts NaCl irrigation treatment on corn planted for 30 days. The treatment group starts spraying 3-(2-aminoethyl)indole spray on the 3rd day after salt treatment, and the control group starts spraying clear water at the same time, and the spraying is carried out continuously for 7 days. The present invention finds that the biomass of the corn plants in the 3-(2-aminoethyl)indole spraying treatment is significantly higher than that of the control group.

[0016] The corn growth regulator provided by the present invention uses 3-(2-aminoethyl)indole as the active ingredient, and the using method is simple, green and economical. It can be applied to the fields of agricultural production and related research to ensure the production of corn crops in saline-alkali environments. Description of the Drawings

[0017] Figure 1 It is the diagram of the growth situation of corn treated with 0.0025 mg / kg, 0.01 mg / kg and 0.025 mg / kg of 3-(2-aminoethyl)indole by mixing at 10 and 20 days of planting.

[0018] Figure 2 It is the influence of 3-(2-aminoethyl)indole applied by mixing at 0.0025 mg / kg, 0.01 mg / kg and 0.025 mg / kg on the plant height of corn at 10 and 20 days of planting.

[0019] Figure 3 The effects of applying 3-(2-aminoethyl)indole at 0.0025 mg / kg, 0.01 mg / kg, and 0.025 mg / kg on the above-ground and underground dry weights of 20-day-old maize.

[0020] Figure 4 The effects of applying 3-(2-aminoethyl)indole at 0.0025 mg / kg, 0.01 mg / kg, and 0.025 mg / kg on the hormone content in the third leaf of maize.

[0021] Figure 5 The effects of spraying 3-(2-aminoethyl)indole at 20 mg / L on the above-ground and underground dry weights of maize under salt stress treatment. Detailed implementation manners

[0022] The present invention provides a maize growth regulator, comprising 3-(2-aminoethyl)indole. The maize growth regulator provided by the present invention uses 3-(2-aminoethyl)indole as an active ingredient and is preferably applied in the form of a 3-(2-aminoethyl)indole solution; the solvent of the 3-(2-aminoethyl)indole solution is preferably water or other agriculturally acceptable solvents. In the present invention, the concentration of the 3-(2-aminoethyl)indole solution is preferably 0.01 - 1 mg / ml.

[0023] The maize growth regulator provided by the present invention has two aspects of applications. On the one hand, it can promote the growth of maize seedlings. Preferably, before planting maize, mixing the soil with the maize growth regulator can significantly increase the above-ground plant height, biomass, and the IAA content in the leaves of the planted maize. On the other hand, the maize growth regulator can improve the salt tolerance of maize. Under salt stress conditions, spraying the maize growth regulator can significantly increase the biomass of maize plants.

[0024] The present invention provides the application of the described maize growth regulator in promoting the growth of maize seedlings.

[0025] In the present invention, the maize growth regulator is preferably used by mixing with soil before planting maize; the application amount of 3-(2-aminoethyl)indole is preferably 0.001 - 0.030 mg / kg of soil. Further, after diluting the 3-(2-aminoethyl)indole solution with water, it is poured onto the soil and stirred evenly; then maize seeds that have germinated are sown and cultivated.

[0026] In the present invention, promoting the growth of maize seedlings includes increasing the biomass of maize seedlings, increasing the plant height of maize seedlings, and / or increasing the auxin content in maize leaves. In the present invention, the auxin is IAA.

[0027] The present invention also provides the application of the described corn growth regulator in improving the salt tolerance of corn.

[0028] In the present invention, the method of using the corn growth regulator is to spray the above-ground part of the corn plant. The corn plant is a corn plant planted for 20 to 40 days. The timing of spraying is from 9:00 to 11:00 in the morning. The spraying state is based on the leaf surface being wet and forming water droplets without falling; the spraying time is continuously spraying for 5 to 10 days, preferably 6 to 8 days, and more preferably 7 days.

[0029] In the present invention, the concentration of the corn growth regulator is preferably 10 to 30 mg / L, further preferably 15 to 25 mg / L, and even more preferably 20 mg / L.

[0030] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0031] Example 1

[0032] This example relates to the application of 3-(2-aminoethyl)indole in soil mixing to promote corn growth.

[0033] 1. Agents, corn materials and seed germination methods

[0034] The pure product of 3-(2-aminoethyl)indole (CAS: 61-54-1) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Aladdin, 98%). It was dissolved in milli Q water to prepare a stock solution with a concentration of 1 mg / mL for subsequent use. The test soil was from a test farmland in Hangzhou, Zhejiang. The test corn variety was B73 (Zea mays). After the seeds were soaked overnight, they were germinated in the dark for 2 days in a petri dish lined with wet filter paper.

[0035] 2. 3-(2-aminoethyl)indole soil mixing experiment treatment

[0036] Take 12.5 μL, 50 μL, and 125 μL of the stock solution of 3-(2-aminoethyl)indole (1 mg / mL) with different volumes respectively, dilute with water to 500 mL, sprinkle it onto 4.5 kg of soil and stir evenly to obtain 5 kg of test soil with the final application concentrations of 3-(2-aminoethyl)indole being 0.0025 mg / kg, 0.01 mg / kg, and 0.025 mg / kg. The control group was set as the soil watered with the same volume of milli Q water. Then, evenly divide the soil into cultivation pots (200 g of soil per pot), sow the germinated maize B73 seeds in the cultivation pots, and each maize plant was treated with 0.0005 mg, 0.002 mg, and 0.005 mg of 3-(2-aminoethyl)indole. The plants were placed in a greenhouse at a temperature of 26.5 °C, a humidity of 55%, and a carbon dioxide concentration of 456 ppm and cultured for 20 days. During this period, water was appropriately added every day to ensure the normal growth of maize.

[0037] 3. Index determination

[0038] Divide the replicates of the same treatment into two parts. One part is used to obtain the growth indexes (plant height, biomass) of maize seedlings, and the other part is used to obtain the hormone indexes of maize leaves.

[0039] Measurement of maize plant height: When the maize seedlings were planted in the soil with different concentrations of 3-(2-aminoethyl)indole for 10 days and 20 days, use a meter stick to measure the plant height of the maize.

[0040] Measurement of maize biomass: After the maize seedlings were planted in the soil with different concentrations of 3-(2-aminoethyl)indole for 20 days, collect the above-ground and underground parts of the maize, dry them, and measure the dry weight.

[0041] Measurement of hormones in maize leaves: After the maize seedlings were planted in the soil with different concentrations of 3-(2-aminoethyl)indole for 10 days, collect the third leaf, grind it in liquid nitrogen, collect 0.1 g of maize leaf powder, and extract 3-indoleacetic acid (auxin, IAA) in the maize leaf powder with an ethyl acetate isotope-labeled product (1 ng d5-IAA), and analyze it by UHPLC-MS / MS to obtain the hormone content indexes in maize after the treatment of mixing and applying 3-(2-aminoethyl)indole.

[0042] 4. Experimental results

[0043] As Figures 1 to 2 shown, the plant height of maize planted in the soil after mixing and applying 3-(2-aminoethyl)indole was significantly higher than that of the non-mixed control group, and it promoted the growth of plants at both time points.

[0044] As Figure 3As shown, compared with the control, applying 3-(2-aminoethyl)indole to the soil significantly increased the biomass of maize. The dry weight of the above-ground part of maize increased by 52.6% - 63.5%, and the highest dry weight increase occurred at the application concentration of 0.0025 mg / kg (i.e., the lowest concentration). Compared with the control, the dry weight of the underground part of maize increased by 19.5% - 55.3%, and the highest dry weight increase occurred at the application concentration of 0.01 mg / kg (i.e., the medium concentration). There was a tendency for the root dry weight to increase in the high-concentration application treatment.

[0045] As Figure 4 shown, compared with the control, applying 0.01 and 0.025 mg / kg of 3-(2-aminoethyl)indole to the soil significantly increased the auxin content in maize leaves.

[0046] Based on the above results, the conclusion is that applying 3-(2-aminoethyl)indole to the soil for maize planting can promote the growth of maize seedlings. Among them, the application at a concentration of 0.0025 mg / kg can promote the growth of the above-ground part to the greatest extent; the application at a concentration of 0.01 mg / kg can promote the growth of the underground part to the greatest extent. In other words, applying 3-(2-aminoethyl)indole at a low concentration can already achieve the effect of promoting the growth of maize seedlings.

[0047] Example 2

[0048] This example relates to the application of spraying 3-(2-aminoethyl)indole to improve the salt tolerance of plants.

[0049] 1. Experimental method and index determination for spraying 3-(2-aminoethyl)indole

[0050] The pure product of 3-(2-aminoethyl)indole, the tested maize seeds and their germination promotion, the tested soil, and the greenhouse conditions refer to Example 1. The germinated maize seedlings were planted in cultivation pots (1 kg of soil per pot). During the 30-day planting period under greenhouse conditions, water was appropriately applied every day.

[0051] Salinization treatment: When the maize was planted for 30 days, the treatment group began to irrigate the soil with 100 mM NaCl solution until saturation, and then an appropriate amount of salt was supplemented to the soil during the planting period to control the soil salinity conductivity at about 1.5 mS / cm; the control group was irrigated and supplemented with clear water.

[0052] 3-(2-Aminoethyl)indole spraying treatment: Dissolve pure 3-(2-aminoethyl)indole in water to prepare a 20 mg / L spray. After irrigating the plants with 100 mM NaCl solution for 3 days, spray the maize at 9:00 - 11:00 am local time in a ventilated place with 3-(2-aminoethyl)indole. The spraying state should be such that the leaves are moist and form water droplets but do not fall off. After the water stains on the leaves dry, move the plants back to the greenhouse for cultivation. This process is carried out continuously for 7 days. The control group sprays with clear water in the upwind direction in the same manner. After 7 days, collect the above-ground and underground parts of the maize plants, dry them, and measure the dry weight.

[0053] 2. Experimental results

[0054] The results are as Figure 5 shown. Spraying with 20 mg / L of 3-(2-aminoethyl)indole can significantly improve the growth of maize and increase the above-ground and underground biomass of maize plants in saline-alkali environments. Compared with the salt stress treatment, the above-ground part increased in weight by approximately 27.8%, and the underground part increased in weight by approximately 44.4%.

[0055] In summary, the 3-(2-aminoethyl)indole spraying treatment improves the salt tolerance of maize, thus ensuring maize growth.

[0056] As can be seen from the above embodiments, the maize growth regulator provided by the present invention can significantly increase the above-ground plant height, biomass, and IAA content in leaves of maize. At the same time, the 3-(2-aminoethyl)indole spraying treatment can significantly increase the biomass of maize plants in a salt stress environment.

[0057] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A corn growth regulator, characterized in that, It includes 3-(2-aminoethyl)indole.

2. The corn growth regulator according to claim 1, wherein The 3-(2-aminoethyl)indole is applied in the form of a 3-(2-aminoethyl)indole solution.

3. The corn growth regulator according to claim 2, wherein The concentration of the 3-(2-aminoethyl)indole solution is 0.01 - 1 mg / mL.

4. Use of the corn growth regulator according to any one of claims 1 to 3 in promoting the growth of corn seedlings.

5. The application according to claim 4, characterized in that The corn growth regulator is used by mixing with soil before planting corn; the usage amount of the 3-(2-aminoethyl)indole is 0.001 - 0.030 mg / kg of soil.

6. The application according to claim 4, characterized in that, The promotion of corn seedling growth includes increasing the biomass of corn seedlings, increasing the plant height of corn seedlings and / or increasing the auxin content in corn leaves.

7. Use of the corn growth regulator according to claim 1 or 2 in improving the salt tolerance of corn.

8. The application according to claim 7, characterized in that The usage method of the corn growth regulator is to spray the above-ground part of the corn plant, and the corn plant is a corn plant planted for 20 - 40 days; the spraying time is from 9:00 to 11:00 in the morning; the spraying is carried out continuously for 5 - 10 days.

9. The application according to claim 7, characterized in that, The concentration of the corn growth regulator is 10 - 30 mg / L.

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