AGEs decomposer

Abstract

The present invention relates to a novel AGEs-decomposing agent, which is characterized by containing a compound selected from the group consisting of a rose cv.Plena extract, an Acetylneuraminic acid (NANA, Acetylneuraminic acid), a Camellia japonica flower extract, a Crocus sativus L. extract, a Citrus aurantium L. powder, a nicotinamide mononucleotide (NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN, NMN The invention relates to a compound which is prepared from at least one of the following raw materials: an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae, an extract of flos carnosae rugosae and an extract of radix

Description

Technical Field

[0001] The present invention relates to a new AGEs degrading agent, a cosmetic having an anti-glycation effect and / or a food having an anti-glycation effect containing the new AGEs degrading agent. Background Art

[0002] It is known that oxidation reactions and glycation reactions in the body have an adverse effect on cells and tissues. In particular, proteins affected by oxidation reactions or glycation reactions, that is, oxidized proteins or glycated proteins, the generation and accumulation of such proteins cause diseases such as diabetic complications, Alzheimer's disease, cataracts, arteriosclerosis, etc., as well as the aging and functional decline of tissues such as the skin. Therefore, the above-mentioned oxidized proteins and the above-mentioned glycated proteins are called harmful proteins. In particular, the accumulation of the final product AGEs (advanced glycation end products) generated by the non-enzymatic addition reaction of sugar to proteins is regarded as a problem.

[0003] Generally, these harmful proteins are decomposed and removed by proteases and proteolytic enzymes such as oxidized protein hydrolase (hereinafter referred to as "OPH"). However, the activities of these enzymes decrease with age, so it is expected to treat and prevent the above-mentioned diseases, and treat and prevent the aging of the above-mentioned tissues and the decline of tissue function by enhancing the activities of the above-mentioned enzymes.

[0004] Substances that activate the above-mentioned protease are disclosed in the prior art as soyasaponins, kale extracts, silybum marianum, silane compounds, and acorus calamus extracts, etc. (Patent Documents 1 to 5). In addition, the above-mentioned OPH is a kind of serine protease, and substances that activate it are known as roman chamomile, houttuynia cordata, european hawthorn, and grapes, etc. (Patent Document 6). However, in recent years, there is a need for a new degrading agent that can efficiently decompose AGEs.

[0005] Prior Art Documents

[0006] Patent Documents

[0007] Patent Document 1 Japanese Patent Laid-Open No. 2002-179592

[0008] Patent Document 2 Japanese Patent No. 4255432

[0009] Patent Document 3 Japanese Patent Laid-Open No. 2008-308505

[0010] Patent Document 4 Japanese Patent Laid-Open No. 2004-91398

[0011] Patent Document 5 Japanese Patent Laid-Open No. 2007-99650

[0012] Patent Document 6 WO2011 / 004733 Summary of the Invention

[0013] Technical Problem to be Solved by the Invention

[0014] One object of the present invention is to provide a new AGEs degrading agent, and another object is to provide a cosmetic having an anti-glycation effect and / or a food having an anti-glycation effect containing the new AGEs degrading agent.

[0015] Means for Solving the Problem

[0016] In order to find a new AGEs degrading agent having an AGEs degrading effect, the present inventors conducted a large number of experiments and found that at least one selected from rose (Rose rugosa cv. Plena) extract, acetylneuraminic acid (NANA), tea tree (Camellia sinensis (L.) O. Kuntze) flower extract, saffron (Crocus sativus L.) extract, bitter orange (Citrus aurantium L.) powder, nicotinamide mononucleotide (NMN), dendrobium officinale Kimura et Migo flower extract, yeast fermentation lysate filtrate, carboxycarnosine, camellia japonica seed extract, bupleurum falcatum extract, iris florentina L. root extract has an AGEs degrading effect, can act as an AGEs degrading agent, and can therefore be used in cosmetics and / or foods having an anti-glycation effect, and thus the present invention was completed.

[0017] The present invention provides the following solutions:

[0018] Scheme 1. An AGEs decomposing agent, characterized by containing at least one selected from rose (Rose rugosacv.Plena) extract, acetylneuraminic acid (NANA), tea tree (Camellia sinensis (L.) O.Kuntze) flower extract, saffron (Crocus sativus L.) extract, bitter orange (Citrus aurantium L.) powder, nicotinamide mononucleotide (NMN), dendrobium officinale Kimuraet Migo flower extract, yeast ferment lysate filtrate, carboxycarnosine, camellia japonica seed extract, bupleurum falcatum extract, and iris florentina L. root extract.

[0019] Scheme 2: For the AGEs decomposer described in Scheme 1, in terms of mass%, the active ingredient concentrations are as follows: when the extract of Rose rugosa cv. Plena is used as the active ingredient, its concentration is 0.005 - 1.0%; when Acetylneuraminic acid (NANA) is used as the active ingredient, its concentration is 0.1 - 1.0%; when the extract of Camellia sinensis (L.) O.Kuntze flowers is used as the active ingredient, its concentration is 0.005 - 1.0%; when the extract of Crocus sativus L. is used as the active ingredient, its concentration is 0.005 - 1.0%; when the powder of Citrus aurantium L. is used as the active ingredient, its concentration is 0.005 - 1.0%; when Nicotinamide mononucleotide (NMN) is used as the active ingredient, its concentration is 0.1 - 1.0%; when the extract of Dendrobium officinale Kimura et Migo flowers is used as the active ingredient, its concentration is 0.005 - 1.0%; when the filtrate of yeast fermentation lysate is used as the active ingredient, its concentration is 0.01 - 1.0%; when Carboxycarnosine is used as the active ingredient, its concentration is 0.5 - 1.0%; when the extract of Camellia japonica seeds is used as the active ingredient, its concentration is 0.005 - 1.0%; when the extract of Bupleurum falcatum is used as the active ingredient, its concentration is 0.2 - 1.0%; when the extract of Iris Florentina L. roots is used as the active ingredient, its concentration is 0.1 - 1.0%.

[0020] Scheme 3: The use of the AGEs decomposer described in Scheme 1 for preparing a cosmetic or food having an anti - glycation effect.

[0021] Scheme 4: Use of at least one selected from extracts of Rose rugosa cv. Plena, acetylneuraminic acid (NANA), extracts of flowers of Camellia sinensis (L.) O. Kuntze, extracts of Crocus sativus L., powder of Citrus aurantium L., nicotinamide mononucleotide, extracts of flowers of Dendrobium officinale Kimura et Migo, filtrate of yeast fermentation lysate, carboxycarnosine, extracts of seeds of Camellia japonica, extracts of Bupleurum falcatum, and extracts of roots of Iris Florentina L. as an AGEs degrading agent.

[0022] Scheme 5: Use of at least one selected from extracts of Rose rugosa cv. Plena, acetylneuraminic acid (NANA), extracts of flowers of Camellia sinensis (L.) O. Kuntze, extracts of Crocus sativus L., powder of Citrus aurantium L., nicotinamide mononucleotide, extracts of flowers of Dendrobium officinale Kimura et Migo, filtrate of yeast fermentation lysate, carboxycarnosine, extracts of seeds of Camellia japonica, extracts of Bupleurum falcatum, and extracts of roots of Iris Florentina L. as an AGEs degrading agent in the preparation of a cosmetic or food having an anti-glycation effect.

[0023] Scheme 6: A cosmetic having an anti-glycation effect, characterized by containing the AGEs degrading agent described in Scheme 1 or 2.

[0024] Scheme 7: A food having an anti-glycation effect, characterized by containing the AGEs degrading agent described in Scheme 1 or 2.

[0025] Effects of the Invention

[0026] The present invention first discovers that at least one selected from the extracts of Rose rugosa cv. Plena, acetylneuraminic acid (NANA), the flower extract of Camellia sinensis (L.) O. Kuntze, the extract of Crocus sativus L., the powder of Citrus aurantium L., nicotinamide mononucleotide (NMN), the flower extract of Dendrobium officinale Kimura et Migo, the filtrate of yeast fermentation lysate, carboxycarnosine, the seed extract of Camellia japonica, the extract of Bupleurum falcatum, and the root extract of Iris Florentina L. can decompose AGEs and can be used as an AGEs decomposing agent. Furthermore, the present invention can provide new anti-glycation cosmetics and / or foods containing at least one selected from the extracts of Rose rugosa cv. Plena, acetylneuraminic acid (NANA), the flower extract of Camellia sinensis (L.) O. Kuntze, the extract of Crocus sativus L., the powder of Citrus aurantium L., nicotinamide mononucleotide (NMN), the flower extract of Dendrobium officinale Kimura et Migo, the filtrate of yeast fermentation lysate, carboxycarnosine, the seed extract of Camellia japonica, the extract of Bupleurum falcatum, and the root extract of Iris Florentina L. as an active ingredient. Detailed implementation manners

[0027] The AGEs decomposing agent of the present invention is characterized by containing at least one selected from the group consisting of extracts of Rose rugosa cv.Plena, acetylneuraminic acid (NANA), extracts of flowers of Camellia sinensis (L.) O.Kuntze, extracts of Crocus sativus L., powder of Citrus aurantium L., nicotinamide mononucleotide (NMN), extracts of flowers of Dendrobium officinale Kimura et Migo, filtrate of yeast fermentation lysate, carboxycarnosine, extracts of seeds of Camellia japonica, extracts of Bupleurum falcatum, and extracts of roots of Iris Florentina L.

[0028] The extract of Rose rugosa cv.Plena described in the present invention refers to the extract of rose flowers (double red roses). The main extracts of dried flower buds are rose extraction powder, and the main distilled extracts are rose extraction liquid, rose essential oil, etc. The extract of double red roses is processed by crushing, boiling water extraction, filtration, concentration, blending with or without maltodextrin, spray drying, packaging and other processes using double red roses (Rose rugosa cv.Plena) as raw materials. The rose extract described in the present invention can also use commercially available rose spray-dried instant powder, rose freeze-dried powder and other rose extract products.

[0029] When the AGEs decomposing agent of the present invention is the extract of Rose rugosa cv.Plena, its concentration is 0.005 to 1.0% by mass. This concentration is the effective concentration for verifying the AGEs decomposing activity of the rose extract, preferably 0.01 to 1.0% by mass, more preferably 0.02 to 1.0% by mass. That is, when the extract of Rose rugosacv.Plena of the AGEs decomposing agent of the present invention is applied to cosmetics or foods and expected to exert the AGEs decomposing effect, the active concentration to be achieved in cosmetics or foods is also within the above range.

[0030] The acetylneuraminic acid (NANA, Acetylneuraminic acid) described in the present invention, also known as sialic acid; N-acetylneuraminic acid; muraminic acid; N-acetylsialic acid; O-sialic acid; N-acetyl-neuraminic acid, etc., is a sialic acid monosaccharide commonly present in glycoproteins of cell membranes and glycolipids of mammalian cell gangliosides, and is known to play biological roles in neurotransmission, leukocyte extravasation, viral or bacterial infections. The inventor of the present invention first discovered that acetylneuraminic acid has an AGEs decomposition effect and can be used as an AGEs decomposing agent. Acetylneuraminic acid can use various commercially available products.

[0031] When the AGEs decomposing agent of the present invention is acetylneuraminic acid (NANA, Acetylneuraminic acid), its concentration is 0.1 to 1.0% by mass. This concentration is the effective concentration for verifying that acetylneuraminic acid has AGEs decomposition activity, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the AGEs decomposing agent acetylneuraminic acid of the present invention to cosmetics or foods and expecting it to exert an AGEs decomposition effect, the active concentration to be achieved in cosmetics or foods is also within the above range.

[0032] The tea tree (Camellia sinensis (L.) O.Kuntze) flower extract described in the present invention refers to an extract with the flowers of the tea tree as the extraction part. The tea tree refers to the tea tree (Camellia sinensis (L.) O.Kuntze) of the Camellia family, and its flowers are edible parts. The extraction method is to obtain the tea tree flower extract through processes such as pulverizing the tea tree flowers, extracting with water or ethanol, concentrating, filtering, drying, adding or not adding resistant dextrin, and packaging. Among them, a pure water extract is preferred, and the product is obtained through centrifugal separation, membrane separation, concentration, sterilization, freeze-drying, and metal detection followed by packaging. The tea tree flower extract in the present invention can be any extract from the flower part of the tea tree, and various commercially available products can be used. For example, the freeze-dried instant powder of tea tree flowers from Huayuanlv, product number GFTB01, can be cited.

[0033] When the AGEs decomposing agent of the present invention is the tea tree (Camellia sinensis (L.) O.Kuntze) flower extract, its concentration is 0.005 to 1.0% by mass. This concentration is the effective concentration for verifying that the tea tree flower extract has AGEs decomposition activity, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the AGEs decomposing agent tea tree flower extract of the present invention to cosmetics or foods and expecting it to exert an AGEs decomposition effect, the active concentration to be achieved in cosmetics or foods is also within the above range.

[0034] In the present invention, the saffron (Crocus sativus L.) extract refers to a product extracted from the upper part of the style and stigma of the saffron Crocus sativus L. of the Iridaceae family, and is a product obtained through processing techniques such as pulverizing saffron, extracting with water or ethanol, concentrating, filtering, drying, adding or not adding resistant dextrin, and packaging. The saffron extract in the present invention can use various commercially available products. For example, the saffron extract produced by Shanghai Kaida Biotechnology Co., Ltd. can be cited. It is known that saffron extract is a potent scavenger of hydroxyl radicals and superoxide radicals, can antioxidize, improve mitochondrial function, inhibit apoptosis of optic nerve cells, and also has the effects of improving microcirculation, enhancing immunity, anti-atherosclerosis, antioxidation, reducing blood lipid, promoting bile secretion and protecting the liver, preventing and treating osteoporosis, and protecting the kidneys. The inventor of the present invention first discovered that saffron extract has an AGEs decomposing effect and can be used as an AGEs decomposing agent.

[0035] When the AGEs decomposing agent of the present invention is a saffron (Crocus sativus L.) extract, its concentration is 0.005 - 1.0% by mass. This concentration is the effective concentration for verifying the AGEs decomposing activity of the saffron (Crocus sativus L.) extract, preferably 0.25 - 1.0% by mass, and more preferably 0.5 - 1.0% by mass. When applying the saffron (Crocus sativus L.) extract, which is the AGEs decomposing agent of the present invention, to cosmetics or foods and expecting it to exert an AGEs decomposing effect, the active concentration to be achieved in cosmetics or foods is also within the above range.

[0036] The powder extract of bitter orange flower (Citrus aurantium L.) described in the present invention is an extract obtained from the flower buds of the bitter orange, a dicotyledonous medicinal plant of the Rutaceae family, especially an aqueous extract. The aliases of bitter orange flower also include trifoliate orange flower, sour orange flower, and daidai flower, etc. Commercially available bitter orange flower extracts of various specifications such as 50:1, 20:1, and 10:1 are all available for use. For example, the bitter orange flower powder produced by Qingyunshan Pharmaceutical can be cited. It is known that the whole herb of bitter orange flower contains various components of cardiac glycosides and non-cardiac glycosides and is used in medicine and food in many countries. It is called Adonis amurensis Regel et Radde in traditional Chinese medicine. It has the functions of strengthening the heart, diuresis, sedation, and slowing down the heart rate, can reduce the excitability of the nervous system and the hyperactivity of spinal cord reflex function, and is used for acute diseases and chronic cardiac insufficiency. It is mainly used to treat congestive heart failure, cardiac edema, and atrial fibrillation, and the combination with silver bromide can enhance the therapeutic effect on epilepsy. The inventor of the present invention first discovered that the powder of bitter orange flower has an AGEs decomposing effect and can be used as an AGEs decomposing agent.

[0037] The AGEs decomposing agent of the present invention, when it is powdered bitter orange (Citrus aurantium L.), has a concentration of 0.005 to 1.0% by mass. This concentration is the effective concentration for verifying the AGEs decomposing activity of the powdered bitter orange (Citrus aurantium L.) extract, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the powdered bitter orange (Citrus aurantium L.) extract of the AGEs decomposing agent of the present invention to cosmetics or foods and expecting it to exert an AGEs decomposing effect, the active concentration to be achieved in the cosmetics or foods is also within the above range.

[0038] Nicotinamide mononucleotide (NMN) described in the present invention, whose full name is β-nicotinamide mononucleotide, has the chemical formula C 11 H 15 N2O8P and is a naturally occurring bioactive nucleotide. Structurally, this molecule is composed of a nicotinamide group, a ribose, and a phosphate group. It is known that NMN is an inherent substance in the human body and is also rich in some fruits and vegetables. Since nicotinamide belongs to vitamin B3, NMN belongs to the category of vitamin B derivatives and is widely involved in many biochemical reactions in the human body, being closely related to immunity and metabolism. The inventor of the present invention first discovered that nicotinamide mononucleotide (NMN) has an AGEs decomposing effect and can be used as an AGEs decomposing agent. The nicotinamide mononucleotide (NMN) in the present invention can use various commercially available products.

[0039] The AGEs decomposing agent of the present invention, when it is nicotinamide mononucleotide (NMN), has a concentration of 0.1 to 1.0% by mass. This concentration is the effective concentration for verifying the AGEs decomposing activity of nicotinamide mononucleotide, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the nicotinamide mononucleotide of the AGEs decomposing agent of the present invention to cosmetics or foods and expecting it to exert an AGEs decomposing effect, the active concentration to be achieved in the cosmetics or foods is also within the above range.

[0040] The extract of Dendrobium officinale Kimura et Migo flowers in the present invention refers to the extract obtained by subjecting Dendrobium officinale Kimura et Migo flowers to processes such as pulverization, extraction with water or ethanol, concentration, filtration, drying, with or without addition of resistant dextrin, and packaging. A water extract is preferred. The extract of Dendrobium officinale Kimura et Migo flowers in the present invention can use various commercially available products. For example, Dendrobium officinale Kimura et Migo flower pollen produced by Qingyunshan Pharmaceutical Industry can be cited. In the prior art, it has been reported that Dendrobium officinale has the effects of nourishing yin and promoting the production of body fluids, anti-tumor and anti-mutation, lowering blood sugar, enhancing the body's immunity and antioxidant effects. The inventor of the present invention has for the first time discovered that the extract of Dendrobium officinale Kimura et Migo flowers has an AGEs-decomposing effect and can be used as an AGEs decomposer.

[0041] When the AGEs decomposer of the present invention is the extract of Dendrobium officinale Kimura et Migo flowers, its concentration is 1.0% by mass. This concentration is the effective concentration for verifying the AGEs-decomposing activity of the extract of Dendrobium officinale Kimura et Migo flowers, preferably 0.25 - 1.0% by mass, and more preferably 0.5 - 1.0% by mass. When applying the extract of Dendrobium officinale Kimura et Migo flowers, which is the AGEs decomposer of the present invention, to cosmetics or foods and expecting it to exert an AGEs-decomposing effect, the active concentration to be achieved in cosmetics or foods is also within the above range.

[0042] The filtrate of yeast fermentation lysate product described in the present invention refers to the liquid obtained by subjecting the lysate product produced by yeast fermentation to microfiltration treatment. The filtrate of yeast fermentation lysate product in the present invention can use various commercially available products. For example, it can be cited that the filtrate of yeast fermentation lysate product produced by AC Dermal Respiratory Factor ECM JP, and the whole ingredient composition: filtrate of yeast fermentation lysate product, phenoxyethanol, water. This liquid has various bioactive components, such as polysaccharides, proteins, nucleic acids, etc. It has been reported in the prior art that in the medical field, the filtrate of yeast fermentation lysate product can be used for immunomodulation, anti-tumor, anti-virus and other aspects. In the food field, the filtrate of yeast fermentation lysate product can be used as a natural immune enhancer to enhance human immunity and prevent diseases. It can also be used as a natural antioxidant to reduce the generation of free radicals and protect cells from oxidative damage. In the cosmetic field, it can be used as a natural moisturizer to increase the water content of the skin and improve problems such as dry and rough skin. In addition, it can also be used as a natural antibacterial agent to reduce the reproduction of skin bacteria and prevent skin infections. The inventor of the present invention first discovered that the filtrate of yeast fermentation lysate product has an AGEs decomposition effect and can be used as an AGEs decomposing agent.

[0043] When the AGEs decomposing agent of the present invention is the filtrate of yeast fermentation lysate product, its concentration is 0.01 - 1.0% by mass. This concentration is the effective concentration for verifying the AGEs decomposition activity of the filtrate of yeast fermentation lysate product, preferably 0.25 - 1.0% by mass, and more preferably 0.5 - 1.0% by mass. When expecting the filtrate of yeast fermentation lysate product, the AGEs decomposing agent of the present invention, to exert an AGEs decomposition effect when applied to cosmetics or foods, the active concentration to be achieved in cosmetics or foods is also within the above range.

[0044] The carboxycarnosine described in the present invention, with the scientific name β-alanylhistamine, is an imidazole dipeptide composed of β-alanine and L-histidine. In 1975, carboxycarnosine was first discovered in crustaceans and later also found in the hearts of some mammals. This component is similar in structure to carnosine and has antioxidant and anti-glycation functions. Because it will not be recognized by enzymes in the body and lose its activity, it has better stability. It is known that carboxycarnosine has the function of capturing hydroxyl radicals, singlet oxygen and hydrogen peroxide radicals, can inhibit lipid oxidation caused by non-metal ions, and can stably protect cell membranes. The inventor of the present invention first discovered that carboxycarnosine has an AGEs decomposition effect and can be used as an AGEs decomposing agent. The carboxycarnosine in the present invention can use various commercially available products.

[0045] When the AGEs decomposing agent of the present invention is carboxycarnosine, its concentration is 0.01 to 1.0% by mass. This concentration is the effective concentration for verifying that carboxycarnosine has AGEs decomposing activity, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the AGEs decomposing agent carboxycarnosine of the present invention to cosmetics or foods and expecting it to exert an AGEs decomposing effect, the active concentration to be achieved in the cosmetics or foods is also within the above range.

[0046] The Camellia japonica seed extract in the present invention refers to the Camellia japonica seed extract in the "List of Cosmetic Raw Materials in Use", and various commercially available products can be used. For example, the Camellia japonica seed extract produced by NOF Corporation, CAMELLIA SEED EXTRACT BG-S, can be cited, and the whole ingredient composition is: Camellia japonica seed extract, butylene glycol, water. It is known that the Camellia japonica seed extract has various skin care effects such as anti-aging, anti-pollution, anti-allergic and anti-inflammatory, whitening and moisturizing, and inhibiting sebum secretion. The present inventor first discovered that the Camellia japonica seed extract has an AGEs decomposing effect and can be used as an AGEs decomposing agent.

[0047] When the AGEs decomposing agent of the present invention is the Camellia japonica seed extract, its concentration is 1.0% by mass. This concentration is the effective concentration for verifying that the Camellia japonica seed extract has AGEs decomposing activity, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the AGEs decomposing agent Camellia japonica seed extract of the present invention to cosmetics or foods and expecting it to exert an AGEs decomposing effect, the active concentration to be achieved in the cosmetics or foods is also within the above range.

[0048] The Bupleurum falcatum extract in the present invention refers to the root extract of Bupleurum falcatum. It is known that the root extract of Bupleurum falcatum contains various substances such as volatile oil, saikol, oleic acid, linolenic acid, palmitic acid, stearic acid, lignoceric acid, glucose, and saponins, and has anti-inflammatory and anti-pathogen effects. It is a plant-based natural extract. The main active ingredient, saikosaponin, can enhance the immune system of human cells, improve the skin's metabolic function, increase the permeability of capillaries, and promote the proliferation of cortical cells. Tranexamic acid, glycyrrhizic acid, and urea can all enhance the efficacy of saikosaponin, and their synergy has effects such as wound healing, smoothing rough skin, and preventing skin aging. Saikosaponin can absorb ultraviolet rays, and in vitro tests also show that it has an inhibitory effect on melanin formation. Saikosaponin has good percutaneous permeability and can accelerate the percutaneous penetration of other components. It has strong antioxidant properties, as well as anti-inflammatory and antiviral effects. The uridine it contains can accelerate the metabolism of skin cells and the regeneration of the skin cutin layer. The inventor of the present invention first discovered that the Bupleurum falcatum extract has an AGEs-decomposing effect and can be used as an AGEs decomposer. The Bupleurum falcatum extract in the present invention can use various commercially available products. For example, the Bupleurum falcatum extract produced by Maruzen Pharmaceutical Co., Ltd. can be cited, and its full ingredient composition is: Bupleurum falcatum root extract, butylene glycol, water.

[0049] When the AGEs decomposer of the present invention is the Bupleurum falcatum extract, its concentration is 0.2 to 1.0% by mass. This concentration is the effective concentration for verifying the AGEs-decomposing activity of the Bupleurum falcatum extract, preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When expecting the AGEs-decomposing effect to be exerted by applying the Bupleurum falcatum extract, which is the AGEs decomposer of the present invention, to cosmetics or foods, the active concentration to be achieved in the cosmetics or foods is also within the above range.

[0050] The root extract of Iris Florentina L. described in the present invention is an extract obtained from the roots of Iris Florentina L. as the extraction site. Iris Florentina L. is an ornamental plant with white flowers, mainly distributed in temperate zones. Its roots are rich in isoflavones, which can stimulate skin metabolism, prevent the degradation of skin structural proteins (collagen, elastin), have antioxidant effects, improve the skin's water-locking ability, restore elasticity, and brighten the skin tone. The inventor of the present invention first discovered that the root extract of Iris Florentina L. has an AGEs-decomposing effect and can be used as an AGEs decomposer. The root extract of Iris Florentina L. in the present invention can use various commercially available products. For example, the root extract of Iris Florentina L. produced by Koka Kogyo Co., Ltd. can be cited, and the whole ingredient composition is: the root extract of Iris Florentina L., butylene glycol, water.

[0051] When the AGEs decomposer of the present invention is the root extract of Iris Florentina L., its concentration is 0.1 to 1.0% by mass. This concentration is the effective concentration for verifying the AGEs-decomposing activity of the root extract of Iris Florentina L., preferably 0.25 to 1.0% by mass, and more preferably 0.5 to 1.0% by mass. When applying the root extract of Iris Florentina L., the AGEs decomposer of the present invention, to cosmetics or foods and expecting it to exert an AGEs-decomposing effect, the active concentration to be achieved in the cosmetics or foods is also within the above range.

[0052] The AGEs decomposer of the present invention can contain one of the above active ingredients or can be used in combination of two or more.

[0053] In the present invention, the above extract can be obtained, for example, by extracting from a part or the whole of the above plants. The extraction method is not particularly limited. For example, known methods such as solvent extraction, pressing method, enzyme extraction, microwave-assisted extraction, and ultrasonic-assisted extraction can be cited.

[0054] The solvents used in the above solvent extraction method are not particularly limited, and examples include aqueous solvents, organic solvents, etc. The above aqueous solvents are not particularly limited, and examples include water such as purified water, ion-exchanged water, tap water, etc. The above organic solvents are not particularly limited, and examples include, for example, lower alcohols, polyhydric alcohols, ketones, esters, ethers, nitriles, aromatic compounds, alkyl chlorides, etc. As the above lower alcohols, examples include methanol, ethanol, absolute ethanol, etc. As the above polyhydric alcohols, examples include propylene glycol, 1,3-butanediol, 1,4-butanediol, pentanediol, hexanediol, etc. As the above ketones, examples include acetone, methyl ethyl ketone, etc. As the above esters, examples include ethyl acetate, etc. As the above ethers, examples include methyl ethyl ether, diethyl ether, etc. As the above nitriles, examples include acetonitrile, etc. As aromatic compounds, examples include benzene, toluene, xylene, etc. As the above alkyl chlorides, examples include chloroform, etc.

[0055] One kind of the above extraction solvent can be used, or two or more kinds can be used in combination. In addition, the above solvent can also be a mixed solvent of an aqueous solvent and an organic solvent. The above mixed solvent is not particularly limited, and examples include lower alcohol aqueous solutions such as ethanol aqueous solution. The proportion of the organic solvent in the above mixed solvent is not particularly limited. For example, it can be 1 to 99% by volume.

[0056] Regarding the above solvent extraction method, for example, the extraction part or the whole plant can be used as the raw material, and it can be impregnated in the above extraction solvent for implementation. The above raw material, for example, can also be subjected to treatments such as washing, drying, and pulverization before the above impregnation. When there are two or more kinds of the above raw materials, each raw material can be extracted separately, or a mixture of two raw materials can be subjected to extraction treatment. In the case of extracting each raw material separately, for example, the obtained extracts can also be mixed to prepare a mixed extract. The mixture of the extracts in the above mixed extract is not particularly limited and can be equal amounts (by weight). In the case of extracting after mixing the raw materials, the proportion of each of the above raw materials is not particularly limited. For example, it can be equal amounts (by weight).

[0057] The ratio of the above raw material for extraction to the above extraction solvent is not particularly limited. For example, relative to 100 g (dry weight) of the above raw material, the above extraction solvent can be 1 to 1000 L. Preferably 1 to 100 L. The impregnation time can be appropriately set according to the types and amounts of the above raw material and the above extraction solvent, and there is no particular limitation. Specifically, when 100 g of the above raw material is impregnated in 10 L of the above extraction solvent, the impregnation time is, for example, 0.5 hours or more, preferably 0.5 to 24 hours.

[0058] In addition, the temperature of the extraction solvent during the above extraction can be room temperature, or above or below room temperature, without any particular limitation. When the above extraction solvent is an aqueous solvent, the above extraction treatment is preferably hot water extraction. When using hot water extraction, the temperature of the above aqueous solvent is not particularly limited, for example, it is 30°C or higher, preferably 50 - 100°C. In addition, the treatment time of the above hot water extraction is not particularly limited and can be appropriately set according to the type and dosage of the above raw materials, as well as the dosage of the water solvent, etc., without any particular limitation. Specifically, when 100 g (dry weight) of the above raw materials is immersed in 10 L of the above aqueous solvent, the immersion time is, for example, 0.5 hours or more, preferably 0.5 - 24 hours.

[0059] The above extract can also be subjected to, for example, purification treatment, etc. after extraction. The above purification treatment is not particularly limited, and known methods such as distillation treatment, filtration treatment, chromatography treatment, drying treatment, etc. can be cited.

[0060] The form of the above extract is not particularly limited, and for example, liquid form, paste form, powder form, etc. can be cited, and it can be appropriately selected according to the form of the AGEs decomposing agent.

[0061] The above AGEs decomposing agent can contain only the above extract, or can contain a combination of the above extract and other components. The above other components can also include various additives such as the above OPH activity enhancer, excipient, binder, lubricant, disintegrant, absorption promoter, emulsifier, stabilizer, and preservative. The mixing ratio of various additives in the above AGEs decomposing agent is not particularly limited and can be appropriately set.

[0062] The form of the above AGEs decomposing agent is not particularly limited. For example, solid form, gel form, and liquid form can be cited. As specific examples of the above forms, for example, powder, fine granule, granule, tablet, film-coated tablet, capsule, buccal tablet, eye drop, liquid, patch, lotion, and ointment, etc. can be cited.

[0063] The AGEs decomposing agent of the present invention can also be added to cosmetics or foods for preparing cosmetics or foods having an anti-glycation effect.

[0064] Examples

[0065] Examples are listed below to illustrate the present invention in more detail, but the present invention is not limited to these examples.

[0066] Example 1. Degradation of AGEs - Protein Crosslinking Detection Experiment

[0067] (A) Test Principle

[0068] Advanced glycation end products (AGEs) can form cross-links with collagen, affecting the normal physiological functions of proteins, thereby leading to skin aging, decreased elasticity and gloss. Horseradish peroxidase (HRP) is an enzyme commonly used in enzyme-linked immunosorbent assay technology. 3,3',5,5'-Tetramethylbenzidine (TMB) has higher sensitivity and no carcinogenicity compared to other chromogens in the color reaction system of HRP and is widely used.

[0069] After AGEs are labeled with HPR, AGEs-HPR can react with collagen to form a collagen-AGEs-HPR cross-link. Based on the color reaction of HRP and TMB, TMB can react with the collagen-AGEs-HPR cross-link to produce a dark blue substance. After adding different AGEs cross-link breakers, the collagen-AGEs-HPR cross-link in the system can be decomposed into collagen and AGEs-HRP. Since collagen is coated on the bottom of the 96-well plate, through the plate washing operation, the free AGEs-HRP will be washed away, and only the collagen-AGEs-HPR bound to collagen exists in the system. Therefore, adding AGEs cross-link breakers will cause changes in the quantity of HRP, so there will be further color differences between TMB and HPR. By observing the color depth of TMB and HPR, the absorbance at 450 nm is measured for judgment, and by setting a control group, the breaking ability of different AGEs cross-link breakers can be quantified through the change in the percentage of absorbance.

[0070] Cross-link breaking ability (%) = (OD value of blank control group - OD value of sample group) / OD value of blank control group * 100%

[0071] (B) Test samples:

[0072] Control

[0073] Blank control: Use water

[0074] Test samples: Refer to the samples in Table 1 below, with sample concentration of 0.5 - 1% by mass

[0075] (C) Operating procedure

[0076] 1. The purified AGEs are labeled with HRP according to the technical manual (GLu-BSA system ELISA kit), and the operation steps of the technical manual are as follows:

[0077] (1) Add 100 μl of washing buffer and a sample solution containing 50 - 200 μg of AGEs to the filter tube.

[0078] (2) After centrifuging at 8,000 - 10,000 g for 10 minutes, add 100 μl of washing buffer and centrifuge again.

[0079] (3) Add 10 μl of reaction buffer to the NH2-reactive peroxidase and dissolve it by pipetting up and down.

[0080] (4) Transfer the solution containing NH2-reactive peroxidase to the membrane of the filter tube where AGEs are concentrated.

[0081] (5) Pipette the solution to aspirate and blow it evenly across the entire membrane surface, then place the filter tube in an incubator and incubate at 37 °C for 2 hours.

[0082] (6) Add 100 μl of washing buffer to the filter tube. If the volume of the filtrate exceeds 300 μl, discard the filtrate before proceeding to step 7.

[0083] (7) Centrifuge at 8,000 - 10,000 g for 10 minutes.

[0084] (8) Add 200 μl of Storage buffer and pipette up and down 10 to 15 times to recover the labeled product. Transfer the solution to a 0.5 ml test tube and store it at 0 - 5 °C.

[0085] 2. Use the AGEs-HRP conjugate obtained in step 1 above as reaction substrate S1. Add this reaction substrate S1 to a 96-well plate, add collagen to the reaction substrate S1 as reaction substrate S2, react at 37 °C for 4 hours, then wash to obtain the AGEs-HRP-collagen conjugate. Then add water (as a blank control, BC), various test samples to this conjugate, react at 37 °C for 16 hours, and then wash. Then, add TMB to detect HRP and measure the OD value at 450 nm. The decomposition rate of AGEs-induced collagen crosslinking = (absorbance OD value of BC - absorbance OD value of the sample) / absorbance OD value of BC × 100

[0086] Test results

[0087]

[0088] From the above experimental results, it can be seen that extracts of Rose rugosa cv.Plena, NANA (Acetylneuraminic acid), extracts of Camellia japonica flowers, extracts of Crocus sativus L., powder of Citrus aurantium L., NMN (Nicotinamide mononucleotide), extracts of Dendrobium officinale Kimuraet Migo flowers, filtrate of yeast fermentation lysate, Carboxycarnosine, extracts of Camellia japonica seeds, extracts of Bupleurum falcatum, and extracts of Iris Florentina L. roots all have the effect of decomposing AGEs and can be used as AGEs decomposing agents. Therefore, one or more of them can be used to prepare cosmetics or foods with anti-glycation effects.

[0089] Industrial applicability

[0090] The present inventors first discovered that at least one selected from extracts of Rose rugosa cv.Plena, NANA (Acetylneuraminic acid), extracts of Camellia japonica flowers, extracts of Crocus sativus L., powder of Citrus aurantium L., NMN (Nicotinamide mononucleotide), extracts of Dendrobium officinale Kimuraet Migo flowers, filtrate of yeast fermentation lysate, Carboxycarnosine, extracts of Camellia japonica seeds, extracts of Bupleurum falcatum, and extracts of Iris Florentina L. roots has the effect of decomposing AGEs and can be used as an AGEs decomposing agent, and verified the effect. Therefore, one or more of them can be used to prepare cosmetics or foods with anti-glycation effects.

Claims

1. An AGEs decomposing agent, characterized in that, Comprising at least one selected from the group consisting of extracts of Rose rugosa cv. Plena, N-acetylneuraminic acid, extracts of Camellia japonica flowers, extracts of Crocus sativus L., powder of Citrus aurantium L., nicotinamide mononucleotide, extracts of Dendrobium officinale Kimura et Migo flowers, filtrate of yeast fermented lysate, decarboxylated carnosine, extracts of Camellia japonica seeds, extracts of Bupleurum falcatum, and extracts of Iris Florentina L. roots.

2. The AGEs decomposer according to claim 1, wherein the concentration of the active ingredient is as follows in mass %: when the extract of Rose rugosa cv. Plena is used as the active ingredient, the concentration is 0.005 - 1.0%; when N-acetylneuraminic acid is used as the active ingredient, the concentration is 0.1 - 1.0%; when the extract of Camellia japonica flowers is used as the active ingredient, the concentration is 0.005 - 1.0%; when the extract of Crocus sativus L. is used as the active ingredient, the concentration is 0.005 - 1.0%; when the powder of Citrus aurantium L. is used as the active ingredient, the concentration is 0.005 - 1.0%; when nicotinamide mononucleotide is used as the active ingredient, the concentration is 0.1 - 1.0%; when the extract of Dendrobium officinale Kimura et Migo flowers is used as the active ingredient, the concentration is 0.005 - 1.0%; when the filtrate of yeast fermented lysate is used as the active ingredient, the concentration is 0.01 - 1.0%; when decarboxylated carnosine is used as the active ingredient, the concentration is 0.5 - 1.0%; when the extract of Camellia japonica seeds is used as the active ingredient, the concentration is 0.005 - 1.0%; when the extract of Bupleurum falcatum is used as the active ingredient, the concentration is 0.2 - 1.0%; when the extract of Iris Florentina L. roots is used as the active ingredient, the concentration is 0.1 - 1.0%.

3. The use of the AGEs decomposer according to claim 1 for the preparation of a cosmetic or food having an anti-glycation effect.

5. Use of at least one selected from extracts of Rose rugosa cv. Plena, N-acetylneuraminic acid, extracts of flowers of Camellia japonica, extracts of Crocus sativus L., powder of Citrus aurantium L., nicotinamide mononucleotide, extracts of flowers of Dendrobium officinale Kimura et Migo, filtrate of yeast fermentation lysate, decarboxylated carnosine, extracts of seeds of Camellia japonica, extracts of Bupleurum falcatum, extracts of roots of Iris Florentina L. as an AGEs decomposing agent.

6. Use of at least one selected from extracts of Rose rugosa cv. Plena, N-acetylneuraminic acid, extracts of flowers of Camellia japonica, extracts of Crocus sativus L., powder of Citrus aurantium L., nicotinamide mononucleotide, extracts of flowers of Dendrobium officinale Kimura et Migo, filtrate of yeast fermentation lysate, decarboxylated carnosine, extracts of seeds of Camellia japonica, extracts of Bupleurum falcatum, extracts of roots of Iris Florentina L. as an AGEs decomposing agent in the preparation of a cosmetic or food having an anti-glycation effect.

6. A cosmetic with anti-glycation effect, characterized in that Containing the AGEs decomposing agent according to claim 1 or 2.

7. A food with anti-glycation effect, characterized in that Containing the AGEs decomposing agent according to claim 1 or 2.

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