Preparation method and application of multi-effect antrodia camphorata composite drop granules

Abstract

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method and application of multi-effect Antrodia camphorata composite drop granules. The preparation method comprises the following steps: (1) preparation of Antrodia camphorata extract; (2) preparation of mixed extract of Aspidistra elatior and Schisandra chinensis; (3) preparation of mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos; (4) mixing of the Antrodia camphorata extract, the mixed extract of Aspidistra elatior and Schisandra chinensis, the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain a mixed powder; (5) heating of polyethylene glycol to be completely melted into a liquid, addition of the mixed powder, uniform mixing, injection into a spherical mold, cooling into a pill, demolding, drying, and obtaining of the multi-effect Antrodia camphorata composite drop granules. The Antrodia camphorata extract prepared in the application has a high total triterpene content, the multi-effect Antrodia camphorata composite drop granules have a quick sobering effect, and simultaneously have a liver protection effect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a preparation method and application of multi-effect Antrodia cinnamomea composite drop granules. BACKGROUND

[0002] Antrodia cinnamomea, also known as Antrodia camphorata, red Antrodia or fragrant coniferous fungus, grows on camphor trees. This fungus has attracted widespread attention due to its significant medicinal value, especially in Asian regions, where it is considered one of the rare medicinal materials. Antrodia cinnamomea contains various active ingredients, such as triterpenoids, polysaccharides, superoxide dismutase, etc., which endow it with multiple biological activities such as antioxidant, anti-inflammatory, immune regulation, and liver protection. Traditionally, Antrodia cinnamomea has been used to treat various diseases, including liver disease, cancer adjuvant therapy, and improving blood circulation, etc.

[0003] Antrodia cinnamomea is believed to have certain alcoholism-relieving effects, but the existing alcoholism-relieving drugs containing Antrodia cinnamomea on the market have some problems in actual application, resulting in unsatisfactory alcoholism-relieving effects. The existing Antrodia cinnamomea products generally have the problems of slow alcoholism-relieving speed and insignificant medicinal effect, which are difficult to meet the actual demand. In view of the damage caused by harmful substances produced in the process of alcohol metabolism to the liver, it is necessary to simultaneously protect the liver during alcoholism relief, which not only helps to alleviate the symptoms of acute alcoholism, but also prevents or reduces chronic liver damage that may be caused by long-term drinking. However, the liver protection effect of the existing Antrodia cinnamomea products is also unsatisfactory. SUMMARY

[0004] The purpose of the present application is to provide a preparation method and application of multi-effect Antrodia cinnamomea composite drop granules.

[0005] In order to achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0006] A preparation method of multi-effect Antrodia cinnamomea composite drop granules, comprising the following steps:

[0007] (1) Preparation of Antrodia cinnamomea extract: inoculate Antrodia cinnamomea strain seed liquid into fermentation medium, shake culture, obtain fermentation broth, filter, collect mycelium and fermentation broth respectively, dry the mycelium, mix the dried mycelium with ethanol, heat to reflux extraction, concentrate and dry to obtain Antrodia cinnamomea extract;

[0008] (2) Preparation of mixed extract of Rhizoma Bletillae and Schisandra chinensis: mix Rhizoma Bletillae and Schisandra chinensis with a weight ratio of 1:(2-5), crush, add water, add Lactobacillus plantarum, ferment, sterilize, filter and dry to obtain the mixed extract of Rhizoma Bletillae and Schisandra chinensis;

[0009] (3) Preparation of the mixed extract of dandelion, kudzu vine and tuckahoe: the dandelion, kudzu vine and tuckahoe are mixed in a weight ratio of (3-4):1:(1.5-2), then ground, added with water, added with neutral protease, enzymolysed, inactivated, filtered, dried, and the mixed extract of dandelion, kudzu vine and tuckahoe is obtained;

[0010] (4) The mixed powder is obtained by mixing the extract of Antrodia cinnamomea, the mixed extract of Gynura japonica, Fatsia japonica and Schisandra chinensis, the mixed extract of dandelion, kudzu vine and tuckahoe;

[0011] (5) The polyethylene glycol is heated to be completely melted into liquid, the mixed powder is added, mixed, injected into a spherical mold, cooled into pills, demolded, dried, and the multi-effect Antrodia cinnamomea compound dropping pills are obtained.

[0012] Preferably, 10 parts by weight of the extract of Antrodia cinnamomea, 1.5-4.5 parts by weight of the mixed extract of Gynura japonica, Fatsia japonica and Schisandra chinensis, and 4-7 parts by weight of the mixed extract of dandelion, kudzu vine and tuckahoe are mixed to obtain the mixed powder.

[0013] Preferably, the fermentation medium comprises: deionized water as medium, 10-15 g / L of glucose, 15-20 g / L of sucrose, 2-6 g / L of beef extract, 0.8-1.2 g / L of fatty acid, 12-17 g / L of gluten, 8-13 g / L of corn flour, 0.5-1.5 g / L of potassium dihydrogen phosphate, 0.5-1.5 g / L of magnesium sulfate heptahydrate, and 1-2 g / L of calcium sulfate, sterilized at 121℃ for 20 min.

[0014] Preferably, the fatty acid comprises oleic acid, linoleic acid and stearic acid in a weight ratio of 1:(1.2-1.5):(0.3-0.5).

[0015] Preferably, the amount of Lactobacillus plantarum is 10 7 -10 8 CFU / mL of water.

[0016] Preferably, the fermentation condition is 30-35℃ for 15-20 h.

[0017] Preferably, the amount of neutral protease is 150-200 U / mL of water.

[0018] Preferably, the enzymolysis condition is 40-45℃ for 3-5 h.

[0019] Preferably, the amount of polyethylene glycol is 1.5-2 times the weight of the mixed powder.

[0020] The present application provides the multi-effect Antrodia cinnamomea compound dropping pills prepared according to the preparation method.

[0021] The application provides application of the multi-effect Antrodia camphorata compound drop granules in preparation of alcoholism relieving and liver protecting drugs.

[0022] Compared with the prior art, the application has the following advantages and beneficial effects:

[0023] 1. By adding a specific content of fatty acid in the fermentation medium of the Antrodia camphorata strain, the content of total triterpenes in the Antrodia camphorata extract can be increased, and the alcoholism relieving speed and liver protecting effect of the multi-effect Antrodia camphorata compound drop granules can be improved.

[0024] 2. By mixing the separately prepared Gynura japonica and Schisandra chinensis mixed extract, Taraxacum mongolicum, Pueraria lobata and Poria cocos mixed extract, and the Antrodia camphorata extract, the active components in the extract can assist the total triterpenes in the Antrodia camphorata extract to produce a synergistic effect, and the alcoholism relieving speed and liver protecting effect of the multi-effect Antrodia camphorata compound drop granules can be improved. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the application.

[0026] The raw materials used in the following embodiments of the application are all commercially available goods:

[0027] The Antrodia camphorata strain is available from Beijing Baoebow Biotechnology Co., Ltd. with the item number ATCC200183.

[0028] The preparation method of the seed liquid of the Antrodia camphorata strain comprises the following steps: 1cm 2 (colony area) of the Antrodia camphorata strain is inoculated into PDA culture medium under sterile conditions, and the activated strain is obtained by culturing at 28 DEG C for 7 days; 10mL of the activated strain and 90mL of the seed culture medium are mixed, and the mixture is cultured in a shaking bed under the condition of 120r / min and 28 DEG C for 5 days.

[0029] The PDA slant culture medium is available from Shanghai Yuanye Biotechnology Co., Ltd. with the item number R30024.

[0030] The seed culture medium comprises water as a medium, 28g / L of glucose, 3g / L of protein peptone, 1.2g / L of potassium dihydrogen phosphate and 1.3g / L of magnesium sulfate heptahydrate, and is sterilized at 121 DEG C for 20min.

[0031] The Lactobacillus plantarum is available from Ningbo Tesuotuo Biotechnology Co., Ltd. with the item number TS278112.

[0032] Lactobacillus delbrueckii, product number TS287480, Ningbo Tesco Biotechnology Co., Ltd.

[0033] Neutral protease, product number S10013, Shanghai Yuanye Biotechnology Co., Ltd.

[0034] Example 1

[0035] The present embodiment provides a preparation method of multi-effect Antrodia camphorata compound granules, comprising the following steps:

[0036] (1) Preparation of Antrodia camphorata extract: inoculate the seed liquid of Antrodia camphorata strain into the fermentation medium, the volume ratio of the seed liquid of Antrodia camphorata strain and the fermentation medium is 1:9, cultivate in a shaking bed under the condition of 120 r / min and 28℃ for 7 days to obtain a fermentation broth, filter the fermentation broth with 4 layers of gauze, collect the mycelium and the fermentation broth respectively, dry the mycelium, mix the mycelium and ethanol according to the weight ratio of 1:9, heat to reflux for 80 min, concentrate and dry to obtain the Antrodia camphorata extract;

[0037] The fermentation medium: deionized water as medium, glucose 12 g / L, sucrose 18 g / L, beef extract 3 g / L, fatty acid 1 g / L, gluten powder 15 g / L, corn flour 10 g / L, potassium dihydrogen phosphate 1 g / L, magnesium sulfate heptahydrate 1 g / L, calcium sulfate 1.6 g / L, sterilized at 121℃ for 20 min. The fatty acid includes oleic acid, linoleic acid and stearic acid in a weight ratio of 1:1.4:0.4.

[0038] (2) Preparation of mixed extract of Asparagus cochleatus and Schisandra chinensis: mix Asparagus cochleatus and Schisandra chinensis in a weight ratio of 1:3, crush to less than 100 mesh, add 10 times the weight of water of the total weight of Asparagus cochleatus and Schisandra chinensis, add Lactobacillus plantarum, the amount of Lactobacillus plantarum is 10 8 CFU / mL water, ferment at 32℃ for 18 h, sterilize, filter and dry to obtain the mixed extract of Asparagus cochleatus and Schisandra chinensis;

[0039] (3) Preparation of mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos: mix Taraxacum mongolicum, Pueraria lobata and Poria cocos in a weight ratio of 3.5:1:1.9, crush to less than 100 mesh, add 10 times the weight of water of the total weight of Taraxacum mongolicum, Pueraria lobata and Poria cocos, add neutral protease, the amount of neutral protease is 180 U / mL water, enzyme hydrolysis at 42℃ for 4 h, inactivate the enzyme, filter and dry to obtain the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos;

[0040] (4) Mix 10 parts by weight of Antrodia camphorata extract, 3 parts by weight of mixed extract of Asparagus cochleatus and Schisandra chinensis, and 5 parts by weight of mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain a mixed powder;

[0041] (5) Take 1.5 times the weight of the mixed powder of polyethylene glycol 6000 heated to melt into a liquid, add the mixed powder, mix evenly, and inject into the spherical mold at 80℃ with a quantitative pump, cool to 2℃ to form a pill, demold, and dry at 20℃ for 100 min to obtain multi-effect Antrodia camphorata composite drop pills with a single grain weight of 60 mg.

[0042] Example 2

[0043] The present embodiment provides a preparation method of multi-effect Antrodia camphorata composite drop pills, comprising the following steps:

[0044] (1) Preparation of Antrodia camphorata extract: inoculate the Antrodia camphorata strain seed liquid into the fermentation medium, the volume ratio of the Antrodia camphorata strain seed liquid to the fermentation medium is 1:9, and the seed liquid is cultured in a shaking bed at 120 r / min and 28℃ for 7 days to obtain a fermentation liquid. The fermentation liquid is filtered with 4 layers of gauze, and the mycelium and the fermentation liquid are collected respectively. The mycelium is dried, mixed with ethanol according to a weight ratio of 1:10, heated to reflux for 90 min, concentrated and dried to obtain the Antrodia camphorata extract;

[0045] The fermentation medium: deionized water as medium, glucose 10 g / L, sucrose 20 g / L, beef extract 2 g / L, fatty acid 1.2 g / L, gluten powder 12 g / L, corn flour 13 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 1.5 g / L, calcium sulfate 1 g / L, sterilized at 121℃ for 20 min. The fatty acid includes oleic acid, linoleic acid and stearic acid in a weight ratio of 1:1.2:0.5.

[0046] (2) Preparation of mixed extract of Asparagus adscendens and Schisandra chinensis: mix Asparagus adscendens and Schisandra chinensis in a weight ratio of 1:2, crush to less than 100 mesh, add 10 times the weight of water of the total weight of Asparagus adscendens and Schisandra chinensis, add Lactobacillus plantarum, the amount of Lactobacillus plantarum is 10 7 CFU / mL water, ferment at 35℃ for 15h, sterilize, filter and dry to obtain the mixed extract of Asparagus adscendens and Schisandra chinensis;

[0047] (3) Preparation of mixed extract of dandelion, kudzu root and Poria cocos: mix dandelion, kudzu root and Poria cocos in a weight ratio of 3:1:2, crush to less than 100 mesh, add 10 times the weight of water of the total weight of dandelion, kudzu root and Poria cocos, add neutral protease, the amount of neutral protease is 200 U / mL water, enzyme hydrolysis at 40℃ for 5h, inactivate the enzyme, filter and dry to obtain the mixed extract of dandelion, kudzu root and Poria cocos;

[0048] (4) Mix 10 parts by weight of Antrodia camphorata extract, 1.5 parts by weight of mixed extract of Asparagus adscendens and Schisandra chinensis, and 7 parts by weight of mixed extract of dandelion, kudzu root and Poria cocos to obtain a mixed powder;

[0049] (5) Take 1.5 times the weight of the mixed powder of polyethylene glycol 6000 heated to completely melt into a liquid, add the mixed powder, mix evenly, and inject into the spherical mold at 80°C with a quantitative pump, cool to 2°C to form pills, demold, and dry at 20°C for 100 min to obtain multi-effect Antrodia camphorata composite drop pills with a single pill weight of 60 mg.

[0050] Comparative Example 1

[0051] The difference between this comparative example and Example 1 is that the fatty acid is replaced by linolenic acid.

[0052] Comparative Example 2

[0053] The difference between this comparative example and Example 1 is that the fatty acid includes oleic acid, linoleic acid, and stearic acid in a weight ratio of 1.2:0.5:1.

[0054] Comparative Example 3

[0055] The difference between this comparative example and Example 1 is that the fatty acid includes linoleic acid, linolenic acid, and oleic acid in a weight ratio of 1:1.2:0.5.

[0056] Comparative Example 4

[0057] The difference between this comparative example and Example 1 is that the Lactobacillus plantarum is replaced by Lactobacillus delbrueckii.

[0058] Comparative Example 5

[0059] The difference between this comparative example and Example 1 is that a multi-effect Antrodia camphorata composite drop pill preparation method includes the following steps:

[0060] (1) Preparation of Antrodia camphorata extract: inoculate the Antrodia camphorata strain seed liquid into the fermentation medium, the volume ratio of the Antrodia camphorata strain seed liquid to the fermentation medium is 1:9, and the seed liquid is cultured in a shaking bed at 120 r / min and 28°C for 7 days to obtain a fermentation liquid. The fermentation liquid is filtered with 4 layers of gauze, and the mycelium and fermentation liquid are collected separately. The mycelium is dried, mixed with ethanol according to a weight ratio of 1:10, heated to reflux for 90 min, concentrated, and dried to obtain the Antrodia camphorata extract.

[0061] The fermentation medium: deionized water as medium, glucose 10 g / L, sucrose 20 g / L, beef extract 2 g / L, fatty acid 1.2 g / L, gluten powder 12 g / L, corn flour 13 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 1.5 g / L, calcium sulfate 1 g / L, and sterilized at 121°C for 20 min. The fatty acid includes oleic acid, linoleic acid, and stearic acid in a weight ratio of 1:1.2:0.5.

[0062] (2) mix the stone lotus and schisandra fruit in a weight ratio of 1:2, and pulverize to less than 100 mesh to obtain powder A; mix the dandelion, kudzu vine and poria cocos in a weight ratio of 3:1:2, and pulverize to less than 100 mesh to obtain powder B, mix 1.5 parts by weight of powder A and 7 parts by weight of powder B, add water in an amount of 10 times the total weight of the powders, and add lactobacillus in an amount of 10 7 CFU / mL water, and ferment at 35°C for 15 hours, sterilize, filter, and dry to obtain a mixed extract;

[0063] (3) mix 10 parts by weight of the antrodia cinnamomea extract and 8.5 parts by weight of the mixed extract to obtain a mixed powder;

[0064] (4) take 1.5 times the weight of the mixed powder, heat to melt into a liquid, add the mixed powder, mix well, inject into a spherical mold at 80°C using a quantitative pump, cool at 2°C to form a pill, demold, and dry at 20°C for 100 minutes to obtain multi-effect antrodia cinnamomea composite drop pills with a single pill weight of 60 mg.

[0065] Comparative Example 6

[0066] The difference between this comparative example and Example 1 is that the preparation method of the multi-effect antrodia cinnamomea composite drop pill comprises the following steps:

[0067] (1) Preparation of the antrodia cinnamomea extract: inoculate the antrodia cinnamomea strain seed liquid into a fermentation medium, the volume ratio of the antrodia cinnamomea strain seed liquid to the fermentation medium is 1:9, and cultivate in a shaking bed at 120 r / min and 28°C for 7 days to obtain a fermentation liquid, filter the fermentation liquid with 4 layers of gauze, collect the mycelium and the fermentation liquid respectively, dry the mycelium, mix the mycelium and ethanol according to a weight ratio of 1:10, heat to reflux for 90 minutes, concentrate and dry to obtain the antrodia cinnamomea extract;

[0068] The fermentation medium: deionized water as medium, glucose 10 g / L, sucrose 20 g / L, beef extract 2 g / L, fatty acid 1.2 g / L, gluten powder 12 g / L, corn flour 13 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 1.5 g / L, calcium sulfate 1 g / L, sterilized at 121°C for 20 minutes. The fatty acid includes oleic acid, linoleic acid and stearic acid in a weight ratio of 1:1.2:0.5.

[0069] (2) mixed the stone orchid and schisandra chinensis in a weight ratio of 1:2, and pulverized to less than 100 mesh to obtain powder A; mixed the dandelion, kudzu root and poria cocos in a weight ratio of 3:1:2, and pulverized to less than 100 mesh to obtain powder B, mixed 1.5 parts by weight of powder A and 7 parts by weight of powder B, added water in an amount of 10 times the total weight of the powders, added neutral protease, the amount of neutral protease was 200 U / mL of water based on the amount of water added, enzymolysis at 40°C for 5h, inactivated the enzyme, filtered, dried to obtain a mixed extract;

[0070] (3) mixed 10 parts by weight of the tiger lactarius extract and 8.5 parts by weight of the mixed extract to obtain a mixed powder;

[0071] (4) heated 1.5 times the weight of the mixed powder of polyethylene glycol 6000 to be completely melted into a liquid, added the mixed powder, mixed uniformly, and injected into a spherical mold at 80°C using a quantitative pump, cooled to 2°C to form pills, demolded, and dried at 20°C for 100 min to obtain multi-effect tiger lactarius composite drop pills with a single pill weight of 60 mg.

[0072] Comparative Example 7

[0073] The difference between this comparative example and Example 1 is that 10 parts by weight of the tiger lactarius extract, 6 parts by weight of the mixed extract of stone orchid and schisandra chinensis, and 2 parts by weight of the mixed extract of dandelion, kudzu root and poria cocos were mixed to obtain a mixed powder.

[0074] Performance test

[0075] The total triterpene yield was determined by the vanillin-glacial acetic acid method. Reference: Measurement method of Hong Wenlong et al. Optimization of Ganoderma lucidum fruiting body triterpene extraction process by response surface method. The specific method is to use oleanolic acid as a standard to draw a standard curve. 0.25 g of mycelium obtained in Examples 1-2 and Comparative Examples 1-3 was mixed with ethanol in a weight ratio of 1:9, heated to reflux for 80 min, concentrated and dried to obtain a tiger lactarius extract. The tiger lactarius extract was added to 0.15 mL of 5% vanillin glacial acetic acid solution, mixed with 0.5 mL of perchloric acid, and then placed in a 70°C water bath for 20 min. Then it was quickly placed in ice water to cool, added glacial acetic acid to 5 mL, quickly mixed the solution, and measured the absorbance at 550 nm. The total triterpene mass concentration was calculated according to the standard curve, and the total triterpene yield calculation formula was: W = (c × V × f) / m × 100%, where c is the total triterpene mass concentration calculated according to the standard curve, μg / mL; V is the volume of the extract, mL; f is the dilution factor; and m is the mass of the mycelium, g.

[0076] Table 1 Total Triterpene Yield Test Results

[0077]

[0078]

[0079] As can be seen from Table 1, the mycelium of Examples 1-2 is fermented using fermentation broth containing specific proportions of linoleic acid, linolenic acid and oleic acid, and the total triterpenoids content in the Antrodia cinnamomea extract prepared by ethanol extraction is high. The high total triterpenoids content in the Antrodia cinnamomea extract can promote the decomposition and excretion of ethanol and its metabolite acetaldehyde in the body, helping to accelerate the body's alcohol elimination speed and thus speed up the process of alcohol elimination. At the same time, triterpenoids have strong antioxidant capacity, which can help reduce oxidative stress caused by alcohol intake, protect cells from free radical damage, indirectly support the liver to quickly recover normal function, and thus may improve the efficiency of alcohol elimination. Triterpenoids have significant anti-inflammatory properties and can regulate the immune system, reduce the inflammatory response caused by alcohol to the liver, and help maintain liver health. Triterpenoids can promote the regeneration of liver cells and help repair damaged liver tissue. This is particularly important for liver damage caused by long-term drinking, as sustained repair mechanisms help prevent the development of more serious liver diseases. At the same time, triterpenoids can also enhance the detoxification function of the liver, enabling the liver to more effectively process and eliminate toxins, including harmful substances such as acetaldehyde produced during alcohol metabolism. Therefore, the higher total triterpenoids content in the Antrodia cinnamomea extract means that it plays an important role in improving the speed of alcohol elimination and improving liver protection effects.

[0080] In Comparative Example 1, linoleic acid was used for fermentation, and the total triterpenoids content in the Antrodia cinnamomea extract was lower than that of Example 1.

[0081] In Comparative Example 2, the fatty acid was replaced by oleic acid, linoleic acid and stearic acid in a weight ratio of 1.2:0.5:1, and the total triterpenoids content in the Antrodia cinnamomea extract was lower than that of Example 1.

[0082] In Comparative Example 3, the fatty acid included linoleic acid, linolenic acid and oleic acid in a weight ratio of 1:1.2:0.5, and the total triterpenoids content in the Antrodia cinnamomea extract was lower than that of Example 1.

[0083] 2. Alcohol elimination effect detection.

[0084] Select 100 rats that have not eaten within 6h, and randomly divide them into 10 groups, with half male and half female in each group; 9 groups correspond to the products prepared in Examples 1-2 and Comparative Examples 1-7, respectively, with a dosage of 0.02g / Kg body weight; 1 group is not administered as a blank group. After 30min of administration, each rat is administered 6g / Kg of 55% edible alcohol; at 0.5h, 1h, 1.5h and 3h after alcohol administration, the rats' blood is taken; gas chromatography is used to determine the change in blood alcohol concentration; the detection results are shown in Table 2 below.

[0085] Table 2 Detection results of blood alcohol concentration change (g / L)

[0086] 0.5h 1h 1.5h 3h Example 1 0.25 0.31 0.17 0.01 Example 2 0.27 0.33 0.19 0.02 Comparative Example 1 0.45 0.58 0.35 0.21 Comparative Example 2 0.43 0.56 0.32 0.18 Comparative Example 3 0.40 0.54 0.30 0.15 Comparative Example 4 0.31 0.43 0.22 0.07 Comparative Example 5 0.36 0.48 0.25 0.11 Comparative Example 6 0.34 0.47 0.24 0.08 Comparative Example 7 0.38 0.50 0.29 0.12 Blank Group 0.89 1.02 0.83 0.57

[0087] From Table 2, it can be seen that the alcoholism relieving effect of Examples 1-2 is faster.

[0088] The alcoholism relieving effect in Comparative Example 1-3 is worse than that in Example 1, which is consistent with the total triterpene content in Table 1.

[0089] In Comparative Example 4, the strain is changed, and the alcoholism relieving effect is worse than that in Example 1, which shows that only the fermentation with a specific strain can better assist the alcoholism relieving effect of the Antrodia cinnamomea extract.

[0090] In Comparative Example 5, only mixed fermentation is performed, and in Comparative Example 6, only mixed enzymolysis is performed, and the alcoholism relieving effect is worse than that in Example 1, which shows that the extract prepared by grouping the raw materials for different extraction methods has more active components that can help relieve alcoholism.

[0091] In Comparative Example 7, the amounts of the mixed extract of Gynura japonica and Schisandra chinensis, the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos are different, and the alcoholism relieving effect of the multi-effect Antrodia cinnamomea compound granules is worse.

[0092] The above is the preferred embodiment of the present application, and it should be noted that for those skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered within the scope of protection of the present application.

Claims

1. A preparation method of multi-effect Antrodia camphorata composite droplets, characterized by, The method comprises the following steps: (1) Preparation of the Antrodia camphorata extract: inoculate the seed liquid of the Antrodia camphorata strain into a fermentation medium, shake and cultivate to obtain a fermentation liquid, filter, collect the mycelium and the fermentation liquid respectively, dry the mycelium, mix the dried mycelium with ethanol, heat and extract, concentrate and dry to obtain the Antrodia camphorata extract; (2) Preparation of the mixed extract of the Asparagus cochleatus and the Schisandra chinensis: mix the Asparagus cochleatus and the Schisandra chinensis at a weight ratio of 1: (2-5), crush, add water, add Lactobacillus plantarum, ferment, sterilize, filter and dry to obtain the mixed extract of the Asparagus cochleatus and the Schisandra chinensis; (3) Preparation of the mixed extract of the Taraxacum mongolicum, the Pueraria lobata and the Poria cocos: mix the Taraxacum mongolicum, the Pueraria lobata and the Poria cocos at a weight ratio of (3-4): 1: (1.5-2), crush, add water, add neutral protease, enzymolysis, inactivate the enzyme, filter and dry to obtain the mixed extract of the Taraxacum mongolicum, the Pueraria lobata and the Poria cocos; (4) Mix the Antrodia camphorata extract, the mixed extract of the Asparagus cochleatus and the Schisandra chinensis and the mixed extract of the Taraxacum mongolicum, the Pueraria lobata and the Poria cocos to obtain a mixed powder; (5) Heat the polyethylene glycol to be completely melted into a liquid, add the mixed powder, mix uniformly, pour into a spherical mold, cool into a pill, demold and dry to obtain the multi-effect Antrodia camphorata compound dropping pill; Mix 10 parts by weight of the Antrodia camphorata extract, 1.5-4.5 parts by weight of the mixed extract of the Asparagus cochleatus and the Schisandra chinensis and 4-7 parts by weight of the mixed extract of the Taraxacum mongolicum, the Pueraria lobata and the Poria cocos to obtain a mixed powder; The fermentation medium comprises deionized water as a medium, 10-15 g / L of glucose, 15-20 g / L of sucrose, 2-6 g / L of beef extract, 0.8-1.2 g / L of fatty acid, 12-17 g / L of gluten, 8-13 g / L of corn flour, 0.5-1.5 g / L of potassium dihydrogen phosphate, 0.5-1.5 g / L of magnesium sulfate heptahydrate and 1-2 g / L of calcium sulfate; The fatty acid comprises oleic acid, linoleic acid and stearic acid at a weight ratio of 1: (1.2-1.5): (0.3-0.5).

2. The preparation method of multi-effect Antrodia camphorata composite droplets according to claim 1, characterized in that, The amount of Lactobacillus plantarum is 10 7 -10 8 CFU / mL water, based on the amount of water added.

3. The method of claim 1, wherein the multi-effect Antrodia cinnamomea compound granules are prepared by the steps of: The fermentation condition is 30-35 °C for 15-20 h.

4. The preparation method of multi-effect Antrodia camphorata composite droplets according to claim 1, characterized in that, The amount of the neutral protease is 150-200 U / mL of water.

5. The method of claim 1, wherein the multi-effect Antrodia cinnamomea compound granules are prepared by the steps of: The enzymolysis condition is 40-45 °C for 3-5 h.

6. The method of claim 1, wherein the multi-effect Antrodia cinnamomea compound granules are prepared by the steps of: The amount of the polyethylene glycol is 1.5-2 times of the weight of the mixed powder.

7. A multi-effect Antrodia camphorata compound dropping pill prepared by the preparation method in any one of claims 1-6.

8. The multi-effect Antrodia camphorata compound dropping pill in claim 7 in the preparation of an alcoholism-relieving and liver-protecting medicine.

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