A cell culture device facilitating replacement of culture medium

Abstract

The application provides a cell culture device convenient for replacing culture solution, and relates to the technical field of cell culture. The device comprises a main thermostat box, an upper communication cover, a middle liquid supply cover plate and a drying box. The inside of the main thermostat box is provided with the upper communication cover. The upper part of the upper communication cover is provided with the middle liquid supply cover plate in a circumferential rotating mode. The outside of the main thermostat box is fixedly provided with the drying box. The lower part of the middle liquid supply cover plate is provided with a lower air guide inclined surface in a central shrinkage conical structure. The lower air guide inclined surface is provided with a lower air guide plate in an annular array mode. The bottom of the lower air guide inclined surface is provided with a first liquid supply pipe and a second liquid supply pipe in a penetrating mode. When dry air flows through the lower culture dish, the evaporation of excess moisture in the culture solution is accelerated, and the problem of nutrient solution dilution caused by moisture accumulation is avoided. The problem that the culture microorganisms are simultaneously extracted when the excess water is mechanically extracted, causing additional loss of the microorganisms, is solved.

Description

Technical Field

[0001] This invention relates to the field of cell culture equipment technology, and in particular to a cell culture device that facilitates the replacement of culture medium. Background Technology

[0002] Cell culture devices provide a temperature-controlled environment for the survival and culture of microorganisms (such as viruses, bacteria, and fungi), helping to isolate and amplify pathogens from clinical samples. For example, by culturing bacteria in a patient's sputum using a cell culture device, we can screen for infection types and test drug sensitivity for diagnosis, enabling analysis of the condition and targeted treatment.

[0003] Microorganisms absorb nutrients through the culture medium in petri dishes. The culture medium for microorganisms needs to be added regularly. The culture medium is composed of various nutrients and water. Existing cell culture devices require multiple additions of culture medium after the nutrients are consumed. The water in the nutrient medium is consumed relatively slowly, resulting in a large accumulation of water. The accumulation of water dilutes the subsequently added nutrient medium. When the excess water is mechanically extracted, the cultured microorganisms are also extracted at the same time, causing additional loss of microorganisms. Summary of the Invention

[0004] This disclosure relates to a cell culture device that facilitates the replacement of culture medium. It employs a dual-drying-box series structure, utilizing the physical adsorption of a desiccant to dry the air. The dried air accelerates the evaporation of excess water in the lower culture dish. As the dried air flows through the lower culture dish, it further accelerates the evaporation of excess water in the culture medium, avoiding the problem of nutrient solution dilution caused by water accumulation. Simultaneously, this design avoids the microbial loss caused by mechanical water extraction in traditional methods. Tests have shown that cell viability can be increased by 15%–20%, and the culture medium replacement cycle can be extended by more than 30%. The first and second supply tubes rotate circumferentially to expand the range of the nutrient solution, ensuring uniform infusion of nutrient solution into the lower culture dish and promoting uniform distribution of the nutrient solution. This ensures a uniform supply of nutrients for viruses, bacteria, and fungi in the lower culture dish, and improves the uniformity of nutrient solution concentration within the culture dish to over 98%.

[0005] In a first aspect, this disclosure provides a cell culture device that facilitates the replacement of culture medium, specifically comprising: a main thermostat, an upper connecting cover, a middle liquid supply cover, and a drying chamber. The upper connecting cover is disposed inside the main thermostat, and the middle liquid supply cover is rotatably disposed on the upper part of the upper connecting cover. The drying chamber is fixedly disposed on the outer side of the main thermostat. A movable door is rotatably disposed in the middle of the main thermostat via a hinge. A ventilation fan is fixedly disposed in the middle of the main thermostat. A liquid delivery pump and a liquid storage tank are fixedly disposed on the top of the main thermostat, and the liquid delivery pump and the liquid storage tank are connected to each other.

[0006] In at least some embodiments, an air inlet is connected to one side of the upper connecting cover, an air outlet is connected to the other side of the upper connecting cover, an upper drive motor is fixedly installed on the top of the upper connecting cover, a lower connecting slide is fixedly installed at the bottom of both the air inlet and the air outlet, and a lower threaded ring is rotatably provided on the lower part of the upper connecting cover, with the lower part of the lower threaded ring threadedly connected to the lower culture dish.

[0007] In at least some embodiments, the lower connecting slide and the main constant temperature chamber are vertically slidably connected, the upper connecting cover and the lower culture dish are both located in the closed space formed by the main constant temperature chamber and the movable door, the air outlet and the ventilation fan hose are connected, the air outlet is connected to the ventilation fan in one direction through a one-way valve, the air inside the ventilation fan cannot flow towards the air outlet, the ventilation fan is connected to the air outlet to draw air, and the dried air inside the drying chamber enters the upper connecting cover through the air inlet.

[0008] In at least some embodiments, the lower part of the liquid supply cover is provided with a lower air guide slope with a conical structure that tapers towards the center. The lower air guide slope is provided with lower air guide plates in a ring array. The bottom of the lower air guide slope is provided with a first liquid supply pipe and a second liquid supply pipe. The upper ends of the first liquid supply pipe and the second liquid supply pipe are connected to the upper connecting branch pipe hose. The upper end of the upper connecting branch pipe and the upper connecting main pipe rotate circumferentially and remain connected.

[0009] In at least some embodiments, the lower air guide slope, lower air guide plate, first liquid supply pipe, and second liquid supply pipe are located inside the upper connecting cover and above the lower culture dish. The lower air guide slope and lower air guide plate are located between the air inlet and the air outlet. The upper drive motor spindle meshes with the middle liquid supply cover plate through gears. The gear meshing of the upper drive motor spindle drives the middle liquid supply cover plate to rotate. The first liquid supply pipe and the second liquid supply pipe rotate circumferentially with the middle liquid supply cover plate.

[0010] In at least some embodiments, the first liquid supply pipe is located in the middle of the lower air guide slope, the second liquid supply pipe is located outside the first liquid supply pipe, the upper connecting pipe is fixed to the top of the main constant temperature chamber, the upper end of the upper connecting pipe is connected to the liquid delivery pump, the first liquid supply pipe and the second liquid supply pipe rotate circumferentially with the middle liquid supply cover, the first liquid supply pipe and the second liquid supply pipe realize circumferential ring liquid supply operation, the circumferential rotation of the first liquid supply pipe and the second liquid supply pipe expands the range of nutrient solution, thereby promoting the uniform distribution of nutrient solution in the lower culture dish.

[0011] In at least some embodiments, an air inlet pipe and an air outlet pipe are respectively connected to both sides of the drying chamber, a middle baffle is fixedly installed on the inner side of the drying chamber, and a desiccant is arranged around the outer side of the middle baffle. Air enters the drying chamber through the air inlet pipe, and the arrangement of the middle baffle and the desiccant causes the air to flow in a U-shaped path to both sides.

[0012] In at least some embodiments, the middle baffle and desiccant are located between the air inlet pipe and the air outlet pipe. The air outlet pipe is connected to the air inlet hose, and the air inlet pipe is connected to the air outlet hose of another drying chamber. The air inlet pipe is equipped with a one-way air inlet valve and is connected to the outside. Through the series connection of the two drying chambers, the moisture content in the air is further reduced, and the dryness of the air is further improved. The dried air inside the drying chamber enters the upper connecting cover through the air inlet. The dried air accelerates the evaporation of excess water in the lower petri dish.

[0013] This invention provides a cell culture device that facilitates the replacement of culture medium, and has the following beneficial effects:

[0014] Nutrient solution enters the lower culture dish through the first and second supply tubes. Simultaneously, the first and second supply tubes rotate circumferentially with the middle supply cover plate. This circumferential rotation expands the range of the nutrient solution, ensuring uniform infusion and distribution of the nutrient solution into the lower culture dish. This guarantees a uniform supply of nutrients for the cultivation of microorganisms such as viruses, bacteria, and fungi, increasing the uniformity of nutrient solution concentration within the culture dish to over 98%. This provides a uniform nutritional environment for these microorganisms, significantly improving cultivation efficiency and cell viability.

[0015] By using a dual-drying-box tandem structure, the air is dried through the physical adsorption of a desiccant. The dried air accelerates the evaporation of excess water in the lower culture dish. As the dried air flows through the lower culture dish, it further accelerates the evaporation of excess water in the culture medium, avoiding the problem of nutrient solution dilution caused by water accumulation. At the same time, this design avoids the microbial loss caused by mechanical water extraction in traditional methods. Tests have shown that cell survival rate can be increased by 15% to 20%, the culture medium replacement cycle can be extended by more than 30%, and the culture cost can be effectively reduced.

[0016] The lower air guide slope and lower air guide plate guide the airflow into the middle liquid supply cover plate, promote the contact between the airflow and the lower culture dish, extend the airflow path, increase the gas-liquid contact area and time, and improve the evaporation efficiency of excess water in the lower culture dish. The water evaporation rate can be increased by 45% compared with traditional culture devices. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings of the embodiments will be briefly described below.

[0018] The accompanying drawings described below are only related to some embodiments of the invention and are not intended to limit the invention.

[0019] In the attached diagram:

[0020] Figure 1 A schematic diagram of the overall structure of this application is shown;

[0021] Figure 2 A schematic diagram of the upper connecting cover structure of this application is shown;

[0022] Figure 3 This paper shows a schematic diagram of the structure of the upper connecting cover in half section.

[0023] Figure 4 A schematic diagram of the structure of the liquid supply cover plate of this application is shown;

[0024] Figure 5 A schematic diagram of the structure of the lower culture dish of this application is shown;

[0025] Figure 6 A structural schematic diagram of the cross-section of the drying oven of this application is shown;

[0026] Figure 7 This invention illustrates a schematic diagram of the structure of two drying ovens connected in series.

[0027] Figure 8 The diagram shows the main thermostatic chamber, the upper connecting cover, and the middle liquid supply cover of this application in their separated states.

[0028] List of reference numerals

[0029] 1. Main constant temperature chamber; 101. Movable chamber door; 102. Ventilation fan; 103. Liquid delivery pump; 104. Liquid storage tank;

[0030] 2. Upper connecting cover; 201. Air inlet; 202. Air outlet; 203. Upper drive motor; 204. Lower connecting slide; 205. Lower culture dish; 206. Lower threaded ring;

[0031] 3. Central liquid supply cover plate; 301. Lower air guide slope; 302. Lower air guide plate; 303. First liquid supply pipe; 304. Second liquid supply pipe; 305. Upper connecting branch pipe; 306. Upper connecting main pipe;

[0032] 4. Drying oven; 401. Air inlet pipe; 402. Air outlet pipe; 403. Middle baffle; 404. Desiccant. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the described embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0034] Example 1: Please refer to Figures 1 to 8:

[0035] This invention proposes a cell culture device that facilitates the replacement of culture medium, comprising: a main thermostat 1, an upper connecting cover 2, a middle liquid supply cover 3, and a drying oven 4. A movable door 101 is rotatably mounted in the middle of the main thermostat 1 via a hinge. A ventilation fan 102 is fixedly mounted in the middle of the main thermostat 1. A liquid delivery pump 103 and a liquid storage tank 104 are fixedly mounted on the top of the main thermostat 1, and the liquid delivery pump 103 and the liquid storage tank 104 are connected. The upper connecting cover 2 is located inside the main thermostat 1. An air inlet 201 is connected to one side of the upper connecting cover 2, and an air outlet 202 is connected to the other side of the upper connecting cover 2. An upper drive motor 203 is fixedly mounted on the top of the upper connecting cover 2. The air inlet 201 and the air outlet 202... The bottom is fixedly equipped with a lower connecting slide column 204. The lower part of the upper connecting cover 2 is circumferentially rotatably equipped with a lower threaded ring 206. The lower part of the lower threaded ring 206 is threadedly connected to the lower culture dish 205. The lower connecting slide column 204 is vertically slidably connected to the main constant temperature chamber 1. The upper connecting cover 2 and the lower culture dish 205 are both in the closed space formed by the main constant temperature chamber 1 and the closed movable chamber door 101. The air outlet 202 is connected to the ventilation fan 102 by a hose. The air outlet 202 is connected to the ventilation fan 102 in one direction through a one-way valve. The air inside the ventilation fan 102 cannot flow towards the air outlet 202. The ventilation fan 102 is connected to the air outlet 202 to draw air. The dried air inside the drying chamber 4 enters the upper connecting cover 2 through the air inlet 201.

[0036] A central liquid supply cover plate 3 is rotatably mounted on the upper part of the upper connecting cover 2. A lower air guide slope 301 with a conical structure tapering towards the center is mounted on the lower part of the central liquid supply cover plate 301. Lower air guide plates 302 are arranged in a circular array on the lower air guide slope 301. A first liquid supply pipe 303 and a second liquid supply pipe 304 are threaded through the bottom of the lower air guide slope 301. The upper ends of the first and second liquid supply pipes 303 and 304 are connected to an upper connecting branch pipe 305. The upper end of the upper connecting branch pipe 305 and the upper connecting main pipe 306 rotate circumferentially and remain connected. The lower air guide slope 301, lower air guide plate 302, first liquid supply pipe 303, and second liquid supply pipe 304 are located inside the upper connecting cover 2 and above the lower culture dish 205. The lower air guide slope 301 and lower air guide plate 302 are located at the air inlet 201 and air outlet 202. Between ports 202, the main shaft of the upper drive motor 203 engages with the liquid supply cover plate 3 via gears. The gear engagement of the main shaft of the upper drive motor 203 drives the liquid supply cover plate 3 to rotate. The first liquid supply pipe 303 and the second liquid supply pipe 304 rotate circumferentially along with the liquid supply cover plate 3. A drying chamber 4 is fixedly installed on the outside of the main constant temperature chamber 1. An air inlet pipe 401 and an air outlet pipe 402 are respectively connected to both sides of the drying chamber 4. A middle baffle 403 is fixedly installed on the inside of the drying chamber 4. A desiccant 404 is arranged around the outside of the middle baffle 403. Air enters the drying chamber 4 through the air inlet pipe 401. The arrangement of the middle baffle 403 and the desiccant 404 causes the air to flow in a U-shaped path to both sides, lengthening the air movement path, increasing the contact area and contact time between the air and the desiccant 404, and improving the drying efficiency.

[0037] In this embodiment, the first liquid supply pipe 303 is located in the middle of the lower air guide slope 301, and the second liquid supply pipe 304 is located outside the first liquid supply pipe 303. The upper connecting pipe 306 is fixed to the top of the main constant temperature chamber 1. The upper end of the upper connecting pipe 306 is connected to the liquid delivery pump 103. The first liquid supply pipe 303 and the second liquid supply pipe 304 rotate circumferentially with the middle liquid supply cover plate 3. The first liquid supply pipe 303 and the second liquid supply pipe 304 realize circumferential ring liquid supply operation. The circumferential rotation of the first liquid supply pipe 303 and the second liquid supply pipe 304 expands the range of nutrient solution and promotes the uniform distribution of nutrient solution in the lower culture dish 205.

[0038] In this embodiment, the middle baffle 403 and the desiccant 404 are located between the air inlet pipe 401 and the air outlet pipe 402. The air outlet pipe 402 is connected to the air inlet 201 via a flexible hose. The air inlet pipe 401 is connected to the air outlet pipe 402 of another drying chamber 4 via a flexible hose. The air inlet pipe 401 is equipped with a one-way air inlet valve and is connected to the outside. Through the series connection of the two drying chambers 4, the moisture content in the air is further reduced, and the dryness of the air is further improved. The dried air inside the drying chamber 4 enters the upper connecting cover 2 through the air inlet 201. The dried air accelerates the evaporation of excess water in the lower petri dish 205, eliminating the need for extracting excess water and avoiding microbial loss caused by mechanical water extraction.

[0039] In Example 2, based on Example 1, multiple upper connecting covers 2 can be installed inside the main constant temperature chamber 1 simultaneously. Multiple upper connecting covers 2 can simultaneously perform multiple cell culture operations, improving work efficiency. Simultaneously, an ultraviolet disinfection box is connected to the air outlet of the ventilation fan 102 to disinfect the exhaust air and prevent the spread of viruses and bacteria. The air inlet of the drying chamber 4 is connected to the ultraviolet disinfection box to ensure clean and sterile air entering the drying chamber 4. The air inlet pipe 401 of the drying chamber 4 and the air outlet pipe 402 of the other drying chamber 4 are connected unidirectionally by the ventilation fan 102. The air inlet pipe 401 is equipped with a one-way air inlet valve. The ventilation fan 102 independently controls the air pressure and drying conditions inside the two drying chambers 4, increasing the gas pressure and drying adsorption efficiency inside the drying chambers 4. The two drying chambers 4 achieve dual drying operations, further reducing humidity in the air. When the dry air flows through the lower culture dish 205, it accelerates the evaporation of excess water in the culture medium, avoiding excessive water accumulation in existing cell culture devices and diluting the subsequently added nutrient solution.

[0040] The working principle of this embodiment is as follows: In the detection of diseases such as pneumonia, samples are taken from locations such as sputum, and then the samples are cultured into cells. After culture, the samples are analyzed to determine the type of bacteria and their drug resistance, enabling targeted diagnosis and treatment. Antibiotics and other drugs are used precisely, reducing the overuse of antibiotics. Samples and nutrient solutions are added to the lower culture dish 205. The middle supply cover 3 is screwed to the lower culture dish 205 through the lower threaded ring 206 to form a closed space. The nutrient solution pump 103 draws the nutrient solution from the storage tank 104 into the upper connecting main pipe 306. The nutrient solution in the upper connecting main pipe 306 flows through the upper connecting branch pipe 305 to the first supply pipe 303 and the second supply pipe 304. The nutrient solution enters the lower culture dish 205 through the lower outlets of the first supply pipe 303 and the second supply pipe 304, realizing the precise use of antibiotics during the cell culture process. The nutrient solution inside the lower culture dish 205 is replenished. Simultaneously, the PLC control module starts the upper drive motor 203 at regular intervals. The gear meshing of the main shaft of the upper drive motor 203 drives the middle liquid supply cover plate 3 to rotate. The first liquid supply pipe 303 and the second liquid supply pipe 304 rotate circumferentially with the middle liquid supply cover plate 3, realizing a circumferential ring liquid supply operation. The circumferential rotation of the first liquid supply pipe 303 and the second liquid supply pipe 304 expands the range of the nutrient solution, realizing the uniform pouring of nutrient solution into the lower culture dish 205, promoting the uniform distribution of nutrient solution in the lower culture dish 205, ensuring the uniform supply of nutrients for viruses, bacteria, and fungi in the lower culture dish 205, and improving the uniformity of nutrient solution concentration in the culture dish to over 98%. This provides a uniform nutritional environment for microorganisms such as viruses, bacteria, and fungi, further improving culture efficiency and cell activity.

[0041] Air enters the drying chamber 4 through the air inlet 401. The middle baffle 403 and desiccant 404 cause the air to flow in a U-shaped path to both sides, lengthening the air movement path, increasing the contact area and contact time between the air and the desiccant 404, and improving drying efficiency. The desiccant 404 adsorbs moisture in the air through physical adsorption, achieving the air drying operation. The two drying chambers 4 are connected in series to further reduce the moisture content in the air and further improve the dryness of the air. At the same time, the PLC control module starts the ventilation fan 102 at regular intervals. The ventilation fan 102 is connected to the air outlet 202 to draw air. The dried air inside the drying chamber 4 enters the upper connecting cover 2 through the air inlet 201. The dried air accelerates the evaporation of excess water in the lower petri dish 205 (for example, if the original relative humidity of the air is 50%, it drops to 5% after drying, and the evaporation rate increases by about 10 times). When the dried air flows through the lower petri dish 205, it accelerates the evaporation of excess water in the culture medium. The evaporation of excess water avoids the accumulation of water in existing cell culture devices after the consumption of nutrients in the culture medium, which dilutes the subsequently added nutrient solution. At the same time, this design avoids the microbial loss caused by mechanical water extraction in traditional methods. Tests have shown that cell survival rate can be increased by 5% to 20%, and the culture medium replacement cycle can be extended by more than 30%, effectively reducing culture costs and culture cycle. The lower air guide slope 301 and lower air guide plate 302 guide the airflow entering the middle liquid supply cover plate 3 from the air inlet 201, guiding the airflow downward, promoting the contact between the airflow and the lower culture dish 205 below, extending the airflow path, increasing the air-liquid contact area and time between the airflow and the liquid inside the lower culture dish 205, and improving the evaporation efficiency of excess water in the lower culture dish 205. Compared with the traditional case where the air inlet 201 and air outlet 202 do not have the lower air guide slope 301 and lower air guide plate 302 in the middle, the water evaporation rate can be increased by 45% compared with the traditional culture device.

[0042] The following points should be noted in this article:

[0043] 1. The accompanying drawings of the embodiments disclosed herein only involve structures relevant to the embodiments disclosed herein; other structures may refer to general designs.

[0044] 2. Where there is no conflict, the embodiments of this disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

[0045] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this disclosure should be included within the scope of protection of this disclosure. Therefore, the scope of protection of this disclosure should be determined by the scope of the claims.

Claims

1. A cell culture apparatus that facilitates medium replacement, comprising: The main constant temperature chamber (1), the upper connecting cover (2), the middle liquid supply cover plate (3) and the drying chamber (4) are characterized in that the upper connecting cover (2) is provided inside the main constant temperature chamber (1), the middle liquid supply cover plate (3) is rotatably provided on the upper part of the upper connecting cover (2), the drying chamber (4) is fixedly provided on the outside of the main constant temperature chamber (1), the lower part of the middle liquid supply cover plate (3) is provided with a lower air guide slope (301) with a conical structure that shrinks towards the center, and the lower air guide slope (301) is provided with a lower air guide plate (302) in a ring array. A first liquid supply pipe (303) and a second liquid supply pipe (304) are installed through the bottom of the lower air guide slope (301). The upper ends of the first liquid supply pipe (303) and the second liquid supply pipe (304) are connected to the upper connecting branch pipe (305) with a flexible hose. The upper end of the upper connecting branch pipe (305) and the upper connecting main pipe (306) rotate circumferentially and remain connected. A movable door (101) is installed in the middle of the main constant temperature chamber (1) through a hinge. A ventilation fan (102) is fixedly installed in the middle of the main constant temperature chamber (1). 1) A liquid delivery pump (103) and a liquid storage tank (104) are fixedly installed on the top. The liquid delivery pump (103) and the liquid storage tank (104) are connected. An air inlet (201) is connected to one side of the upper connecting cover (2), and an air outlet (202) is connected to the other side of the upper connecting cover (2). An upper drive motor (203) is fixedly installed on the top of the upper connecting cover (2). A lower connecting slide column (204) is fixedly installed at the bottom of both the air inlet (201) and the air outlet (202). The lower part of the upper connecting cover (2) is circumferentially rotatable. A lower threaded ring (206) is provided, and the lower part of the lower threaded ring (206) is threadedly connected to the lower culture dish (205). The lower air guide slope (301), the lower air guide plate (302), the first liquid supply pipe (303), and the second liquid supply pipe (304) are located inside the upper connecting cover (2) and above the lower culture dish (205). The lower air guide slope (301) and the lower air guide plate (302) are located between the air inlet (201) and the air outlet (202). The main shaft of the upper drive motor (203) is engaged with the liquid supply cover plate (3) through gear meshing.

2. The cell culture device for easy replacement of culture medium according to claim 1, characterized in that, The lower connecting slide (204) and the main constant temperature chamber (1) are vertically slidably connected. The upper connecting cover (2) and the lower culture dish (205) are both located in the closed space formed by the main constant temperature chamber (1) and the movable door (101). The air outlet (202) is connected to the ventilation fan (102) by a hose. The air outlet (202) is unidirectionally connected to the ventilation fan (102) through a one-way valve.

3. The cell culture device for easy replacement of culture medium according to claim 1, characterized in that, The first liquid supply pipe (303) is located in the middle of the lower air guide slope (301), the second liquid supply pipe (304) is located outside the first liquid supply pipe (303), the upper connecting pipe (306) is fixed to the top of the main constant temperature box (1), and the upper end of the upper connecting pipe (306) is connected to the liquid delivery pump (103).

4. The cell culture device for easy replacement of culture medium according to claim 1, characterized in that, The drying chamber (4) is connected to an air inlet pipe (401) and an air outlet pipe (402) on both sides respectively. A middle baffle (403) is fixedly installed on the inner side of the drying chamber (4), and a desiccant (404) is arranged around the outer side of the middle baffle (403).

5. A cell culture device for easy replacement of culture medium according to claim 4, characterized in that, The middle baffle (403) and desiccant (404) are located between the air inlet pipe (401) and the air outlet pipe (402). The air outlet pipe (402) is connected to the air inlet (201) hose, and the air inlet pipe (401) is connected to the air outlet pipe (402) hose of another drying box (4).

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