Sampling equipment for allergen specific IgE antibody based on chemiluminescence immunoassay

Abstract

The invention relates to the technical field of antibody sampling, in particular to sampling equipment for allergen specificity IgE antibodies based on chemiluminescence immunoassay, which comprises a sampling cabin, a placing table in the sampling cabin, a driving device arranged above the placing table and a tube replacing mechanism arranged on the left side of the placing table, the sampling cabin comprises an upper lifting frame, and an insertion hole is formed in the inner wall of the top end of the sampling cabin. According to the sampling equipment for the allergen-specific IgE antibody based on the chemiluminescence immunoassay, through linkage design of the driving device, horizontal movement of the sliding plate is accurately converted into vertical lifting of the blood collection tube, reliable butt joint of the blood collection tube and the collection tube is ensured, and a piston plate is controlled in cooperation with a first electric push rod; the quantitative extraction of serum and the precise instillation to the microwell plate are automatically completed in the closed sampling cabin, and the process is isolated from environmental interference, so that the sample pollution is effectively prevented, and the IgE antibody detection accuracy based on chemiluminescence immunoassay is remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of antibody sampling, and more specifically, to a sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay. Background Art

[0002] A sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay is a key tool for allergy diagnosis. This device can accurately collect samples such as blood, ensuring the integrity and representativeness of the samples. Through sampling, it provides a reliable basis for subsequent chemiluminescence immunoassay, helps to quickly and accurately detect the level of allergen-specific IgE antibodies, and assists doctors in accurately diagnosing allergic symptoms.

[0003] The patent with the application number CN201820450447.2 discloses a sampling device including a positioning mechanism, a punching mechanism, a frame, and a feeding mechanism. The positioning mechanism includes at least one fixed fixture for fixedly installing a blood spot card. The fixed fixture is fixedly installed at the punching station, and the first punching hole and the second punching hole are coaxially opened on the upper and lower sides of the blood spot card by the fixed fixture; the punching mechanism is in punching cooperation with the blood spot card to obtain a sample to be tested; the fixed fixture is slidably installed on the frame, and the blood spot card of the sampling device is installed on the push-pull member of the feeding mechanism and is transported to the punching station through the push-pull member, so that the sampling device can perform punching operations on the blood spot card to obtain a sample to be tested, which can improve the working efficiency of the sampling device.

[0004] However, the existing devices mainly rely on manual or semi-automatic operations during the serum transfer process, resulting in the samples being exposed to an open environment, being easily contaminated, and having low docking accuracy, which affects the detection accuracy. Secondly, after each row of sample processing is completed, it is necessary to manually replace the collection tube, adjust the position of the microplate, and clean the waste, frequently interrupting the operation, so that the device cannot operate continuously and efficiently. The number of serum samples that can be transferred per unit time is too small to meet the demand for processing a large number of samples, and the continuous operation ability is poor.

[0005] In view of this, we propose a sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay. Summary of the Invention

[0006] The purpose of the present invention is to provide a sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay, and by combining the linkage design of the driving device and the tube-changing mechanism with the end stroke of the transverse movement frame to solve the problems raised in the above background art.

[0007] To achieve the above purpose, the present invention provides the following technical solutions: Sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay, comprising a sampling chamber, a placement table inside it, a driving device arranged above the placement table, and a tube-changing mechanism on the left side of the placement table. The sampling chamber includes a lifting frame, and a jack is provided on the inner wall of its top end; The placement table includes a transverse moving plate, and a number of dial grooves are provided on the bottom surface of the transverse moving plate; The driving device includes a sliding plate, a transverse moving frame sleeved outside it, and a docking frame parallel to the sliding plate. An outward convex rod is provided on the outer wall of the sliding plate, and a plug rod is provided on the right wall of the docking frame. A frame surface inclined groove for the outward convex rod to slide is provided on its rear wall.

[0008] When the above-mentioned transverse moving frame is moved to the rightmost side, the plug rod is inserted into the jack. At this time, the sliding plate moves leftward, the outward convex rod abuts against the frame surface inclined groove, and the plug rod immediately drives the lifting frame to rise; The tube-changing mechanism includes a movable sealing plate and an outward pushing part. The outward pushing part includes an outward pushing frame and an upward inserting block whose top end extends into the dial groove. A guiding groove is provided on the top surface of the outward pushing frame, and a downward convex rod that slides inside the guiding groove is provided on the bottom surface of the movable sealing plate.

[0009] This setting drives the outward pushing part to move backward when the downward convex rod contacts the groove wall of the guiding groove during the leftward movement of the movable sealing plate. During this process, the upward inserting block abuts against the dial groove on the bottom surface of the transverse moving plate, and drives the transverse moving plate to move backward at a fixed distance, so that the next row of sampling holes on the microplate are accurately aligned with the axis of the transverse moving frame, realizing the hole position switching of the sampling holes.

[0010] In the technical solution of the present invention, the sampling chamber further includes a cabin with an open rear end, two sliding rods clamped and fixed to the cabin walls at the left and right ends of the cabin, a collection box slidably connected to the bottom of the cabin, and a cabin door hinged to the open rear end of the cabin.

[0011] In the technical solution of the present invention, a chute for the lifting frame to move up and down is provided on the right side wall of the cabin, a cavity for placing the tube-changing mechanism is provided inside the cabin, and a round hole is provided on the bottom transverse plate of the lifting frame.

[0012] The above setting is to complete the smooth extraction of serum, and a lifting frame that can be driven by the driving device to rise is provided, so that the devices inside the placement table can move up together with it.

[0013] In the technical solution of the present invention, the placement table further includes a microplate placed on the top surface of the transverse moving plate, blood collection tubes and collection tubes arranged in an array on its left and right sides. The transverse moving plate is slidably connected to the inside of the cabin. The distance between several dial grooves on the bottom surface of the transverse moving plate is the same as the distance between adjacent sampling holes on the microplate. A round rod for restricting the position of the microplate is integrally formed on the top surface of the transverse moving plate. The blood collection tubes and collection tubes are both slidably connected to the holes on the transverse plates on both sides of the transverse moving plate. When the lifting frame rises, the corresponding blood collection tubes are driven to rise together.

[0014] The above settings are for realizing the automatic transfer and dispensing of serum samples. By opening regularly distributed dial grooves on the bottom surface of the transverse moving platen and cooperating with the tube changing mechanism, the transverse moving platen can achieve fixed-distance movement.

[0015] In the technical solution of the present invention, the driving device further includes a motor fixedly connected to the outer wall of the cabin by screws, a lead screw coaxially connected to the output shaft of the motor, a first electric push rod fixedly connected to the top surface of the transverse moving frame by screws, a piston plate clamped to the telescopic end of the first electric push rod, and a sleeve sleeved outside the piston plate. The sleeve is clamped and fixed to the bottom surface of the transverse moving frame. The sleeve is used for clamping the collection tube. After the blood collection tube rises with the lifting frame, the needle port at the bottom end of the collection tube is inserted into the serum inside the blood collection tube, and the first electric push rod drives the piston plate to move upward to complete the serum extraction.

[0016] In the technical solution of the present invention, the sliding plate is threadedly connected to the lead screw, the convex rod is integrally formed with the sliding plate, a partition is integrally formed inside the transverse moving frame, and a limiting groove for restricting the moving range of the convex rod is opened on the partition. The transverse moving frame is slidably connected to the outside of two sliding rods, and the docking frame is slidably connected to the inside of the transverse moving frame.

[0017] This setting drives the sliding plate to move left and right through the forward and reverse rotation of the motor, and utilizes the constraint relationship between the convex rod and the inclined groove on the frame surface to realize the two-way precise displacement control of the docking frame. When moving to the right, it forces the docking frame to descend to make way for the transverse moving frame. When moving to the left, it forces the docking frame to rise. Through the linkage of the insertion rod and the insertion hole, the blood collection tube and the collection tube are synchronously lifted and precisely docked, providing a core mechanical action basis for serum extraction.

[0018] In the technical solution of the present invention, the tube changing mechanism further includes four telescopic rods clamped and fixed at the corners of the left side wall of the movable sealing plate, a first spring sleeved outside the telescopic rods, a second electric push rod fixedly connected to the outer wall of the movable sealing plate by screws, and a plugging frame clamped and fixed to the telescopic end of the second electric push rod.

[0019] In the technical solution of the present invention, a plate surface chute is opened on the outer wall of the movable sealing plate for the up and down displacement of the plugging frame to penetrate left and right, and the other end of the telescopic rod is clamped and fixed to the inner wall of the cabin.

[0020] This setting is for completing the automatic installation of the collection tube and the recycling of waste tubes. By controlling the contraction of the telescopic rod of the second electric push rod, the plugging frame is driven to move smoothly, so that the collection tube in the placement table can be sleeved outside the sleeve, and the collection tube outside the sleeve can be pushed into the lower collection box.

[0021] In the technical solution of the present invention, the outer pushing part further includes a plurality of second springs welded to the bottom surface of the upper insertion block and an external connecting frame clamped and fixed to the outer wall of the upper insertion block. The external connecting frame is slidably connected to the inside of the through groove.

[0022] In the technical solution of the present invention, the extrapolation frame is slidably connected to a cavity opened inside the cabin body. A groove is provided on the top surface of the horizontal plate at the right end of the extrapolation frame. The bottom end of the second spring is welded and fixed to the bottom surface of the groove wall of the groove. The top end cross-section of the upper insertion block is in the shape of a right trapezoid, and the inclined end faces forward.

[0023] When the extrapolation part moves backward in this setting, the upper insertion block at its upper end abuts against the groove wall of the dial groove on the bottom surface of the horizontal movement table board. The thrust generated by this abutment precisely dials the entire horizontal movement table board to move backward by a fixed distance, so that the next row of sampling holes on the microplate that have not been sampled automatically move to the working position and are precisely aligned with the movement axis of the horizontal movement frame, preparing for the next round of operation.

[0024] Compared with the prior art, the beneficial effects of the present invention are: 1. The sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay, through the linkage design of the driving device, precisely converts the horizontal movement of the sliding plate into the vertical lifting of the blood collection tube, ensures its reliable docking with the collection tube, and cooperates with the first electric push rod to control the piston plate to automatically complete the quantitative extraction of serum and the precise dripping into the microplate in the airtight sampling cabin. This process isolates environmental interference, effectively prevents sample contamination, and significantly improves the detection accuracy of IgE antibodies based on chemiluminescence immunoassay.

[0025] 2. The sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay, the tube changing mechanism cleverly combines with the end stroke of the horizontal movement frame. When moving left, it pushes the movable sealing plate to trigger the second electric push rod to discard the waste collection tube into the collection box. At the same time, the lower convex rod of the movable sealing plate forcibly drives the extrapolation part to move backward through the guide groove. The upper insertion block thereof abuts against the dial groove to push the horizontal movement table board to move backward by a fixed distance, automatically positioning the next row of sampling holes precisely on the working axis. This mechanism seamlessly connects the waste tube recycling and plate row switching, and can continuously process multiple rows of samples without manual intervention, greatly improving the equipment throughput and operation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is one of the schematic sectional views of the overall structure of the present invention; Figure 3 is another schematic sectional view of the overall structure of the present invention; Figure 4 is one of the schematic sectional views of the structure of the sampling cabin in the present invention; Figure 5 is another schematic sectional view of the structure of the sampling cabin in the present invention; Figure 6 is a schematic diagram of the structure of the lifting frame in the present invention; Figure 7 It is a schematic structural diagram of the placement table in the present invention; Figure 8 It is a schematic structural diagram of the driving device in the present invention; Figure 9 It is one of the schematic sectional views of a part of the structure of the driving device in the present invention; Figure 10 It is a schematic diagram of a part of the structure of the driving device in the present invention; Figure 11 It is the second of the schematic sectional views of a part of the structure of the driving device in the present invention; Figure 12 It is a schematic sectional view of the tube changing mechanism in the present invention; Figure 13 It is a schematic exploded view of the structure of the outer pushing part in the present invention; Explanation of reference numerals: 100, sampling chamber; 110, chamber body; 111, chute; 112, through groove; 120, sliding rod; 130, collection box; 140, lifting frame; 141, round hole; 142, insertion hole; 150, hatch door; 200, placement table; 210, transverse moving table board; 211, dialing groove; 220, microplate; 230, blood collection tube; 240, collection tube; 300, driving device; 310, motor; 320, lead screw; 330, sliding plate; 331, convex rod; 340, transverse moving frame; 341, limiting groove; 350, docking frame; 351, insertion rod; 352, inclined groove on the frame surface; 360, first electric push rod; 370, piston plate; 380, sleeve; 400, tube changing mechanism; 410, movable sealing plate; 411, lower convex rod; 412, chute on the plate surface; 420, telescopic rod; 430, first spring; 440, second electric push rod; 450, insertion frame; 460, outer pushing part; 461, outer pushing frame; 4610, guiding groove; 4611, groove; 462, upper insertion block; 463, second spring; 464, external connecting frame. Detailed implementation manners

[0027] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present invention without creative efforts shall fall within the protection scope of the present invention.

[0028] Please refer to Figures 1-6 as shown, the technical solution provided in this embodiment is as follows: Sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay, comprising a sampling chamber 100, a placement table 200 inside it, a driving device 300 arranged above the placement table 200, and a tube-changing mechanism 400 on the left side of the placement table 200.

[0029] In this embodiment, the sampling chamber 100 includes a lifting frame 140, and a jack 142 is provided on the inner wall of the top end thereof.

[0030] Specifically, the sampling chamber 100 further includes a cabin body 110 with an open rear end, two slide rods 120 clamped and fixed to the left and right end walls of the cabin body 110, a collection box 130 slidably connected to the bottom of the cabin body 110, and a cabin door 150 hinged to the open rear end of the cabin body 110.

[0031] Furthermore, a chute 111 for the lifting frame 140 to move up and down is provided on the right side wall of the cabin body 110, a cavity for placing the tube-changing mechanism 400 is provided inside the cabin body 110, and a round hole 141 is provided on the bottom horizontal plate of the lifting frame 140.

[0032] Furthermore, the cabin body 110 cooperates with the cabin door 150 to ensure the sealing of the internal environment of the sampling chamber 100 during operation. The slide rods 120 are used to limit the movement range of the internal structure of the driving device 300. The collection box 130 is used to recycle the internal devices of the placement table 200. This setting is for smoothly completing serum extraction, and a lifting frame 140 that can be driven by the driving device 300 to rise is provided, so that the internal devices of the placement table 200 can move up together with it.

[0033] Please refer to Figure 7 As shown, in this embodiment, the placement table 200 includes a transverse moving table board 210, and a number of dial grooves 211 are provided on the bottom surface of the transverse moving table board 210.

[0034] Specifically, the placement table 200 further includes a microplate 220 placed on the top surface of the transverse moving table board 210, and blood collection tubes 230 and collection tubes 240 arranged in an array on its left and right sides. The transverse moving table board 210 is slidably connected to the inside of the cabin body 110. The distance between a number of dial grooves 211 on the bottom surface of the transverse moving table board 210 is the same as the distance between adjacent sampling holes on the microplate 220. A round rod for restricting the position of the microplate 220 is integrally formed on the top surface of the transverse moving table board 210. The blood collection tubes 230 and the collection tubes 240 are both slidably connected to the holes on the transverse boards on both sides of the transverse moving table board 210.

[0035] Furthermore, the transverse moving platen 210 is used to provide a placement platform for the microplate 220, the blood collection tube 230, and the collection tube 240. The blood collection tube 230 is used to place the centrifuged blood sample. When the lifting frame 140 rises, it drives the corresponding blood collection tube 230 to rise simultaneously, which is a prerequisite for completing serum extraction. This setting is to achieve the automatic transfer and dispensing of serum samples. By providing regularly distributed dialing grooves 211 on the bottom surface of the transverse moving platen 210 and cooperating with the tube changing mechanism 400, the transverse moving platen 210 can achieve fixed-distance movement.

[0036] Please refer to Figures 8-11 As shown, in this embodiment, the driving device 300 includes a sliding plate 330, a transverse moving frame 340 sleeved outside it, and a docking frame 350 parallel to the sliding plate 330. An outward protruding rod 331 is provided on the outer wall of the sliding plate 330, and an insertion rod 351 is provided on the right wall of the docking frame 350. A frame surface inclined groove 352 for the outward protruding rod 331 to slide is provided on the rear wall thereof. When the transverse moving frame 340 moves to the rightmost side, the insertion rod 351 is inserted into the insertion hole 142. At this time, the sliding plate 330 moves leftward, the outward protruding rod 331 abuts against the frame surface inclined groove 352, and the insertion rod 351 immediately drives the lifting frame 140 to rise.

[0037] Specifically, the driving device 300 further includes a motor 310 fixedly connected to the outer wall of the cabin body 110 by screws, a lead screw 320 coaxially connected to the output shaft of the motor 310, a first electric push rod 360 fixedly connected to the top surface of the transverse moving frame 340 by screws, a piston plate 370 clamped to the telescopic end of the first electric push rod 360, and a sleeve 380 sleeved outside the piston plate 370. The sleeve 380 is clamped and fixed to the bottom surface of the transverse moving frame 340. The sleeve 380 is used to clamp the collection tube 240. After the blood collection tube 230 rises with the lifting frame 140, the needle port at the bottom end of the collection tube 240 is inserted into the serum inside the blood collection tube 230, and the first electric push rod 360 drives the piston plate 370 to move upward to complete serum extraction.

[0038] Furthermore, the sliding plate 330 is threadedly connected to the lead screw 320. The outward protruding rod 331 is integrally formed with the sliding plate 330. A partition is integrally formed inside the transverse moving frame 340, and a limiting groove 341 for restricting the moving range of the outward protruding rod 331 is provided on the partition. The transverse moving frame 340 is slidably connected to the outside of the two sliding rods 120, and the docking frame 350 is slidably connected to the inside of the transverse moving frame 340.

[0039] Further, the motor 310 drives the skateboard 330 to start moving rightward within the track of the transverse movement frame 340. During this process, the convex rod 331 fixed on the skateboard 330 slides along the limit groove 341 on the transverse movement frame 340. When the convex rod 331 contacts the groove wall of the frame surface inclined groove 352 on the side of the docking frame 350, as the skateboard 330 continues to move rightward, the contact position of the convex rod 331 within the frame surface inclined groove 352 changes, and under geometric constraints, it forces the docking frame 350 to generate a downward displacement. After the downward movement of the docking frame 350 is completed, the skateboard 330 immediately pulls the entire transverse movement frame 340 to translate rightward synchronously.

[0040] Further, the transverse movement frame 340 continues to move rightward until it reaches directly above the array of blood collection tubes 230. The insertion rod 351 fixed on the docking frame 350 precisely inserts into the insertion hole 142 opened at the top end of the lifting frame 140. Then, the motor 310 is controlled to reverse. The reverse rotation of the motor 310 drives the skateboard 330 to start moving leftward within the transverse movement frame 340. The convex rod 331 on the skateboard 330 interacts with the groove wall of the frame surface inclined groove 352 of the docking frame 350 again during the leftward movement. Due to the specific angle design of the frame surface inclined groove 352, the movement of the convex rod 331 forces the docking frame 350 to generate an upward displacement. Since the insertion rod 351 has been inserted into the insertion hole 142 of the lifting frame 140, the upward movement of the docking frame 350 directly pulls the lifting frame 140 and the blood collection tube 230 fixed below it to rise together against gravity. The rising blood collection tube 230 precisely inserts outside the needle hole interface at the bottom end of the collection tube 240.

[0041] Further, the telescopic end of the first electric push rod 360 is started to retract, pulling the piston plate 370 connected to it to move upward within the collection tube 240, forming a negative pressure, thereby safely and quantitatively sucking the serum sample in the blood collection tube 230 into the cavity inside the collection tube 240.

[0042] This setting drives the skateboard 330 to move left and right through the forward and reverse rotation of the motor 310, and utilizes the constraint relationship between the convex rod 331 and the frame surface inclined groove 352 to achieve two-way precise displacement control of the docking frame 350. When moving rightward, it forces the docking frame 350 to descend to make way for the transverse movement frame 340. When moving leftward, it forces the docking frame 350 to rise. Through the linkage between the insertion rod 351 and the insertion hole 142, the blood collection tube 230 and the collection tube 240 are synchronously lifted for precise docking, providing the core mechanical action basis for serum extraction.

[0043] Please refer to Figure 12 As shown, in this embodiment, the tube changing mechanism 400 includes a movable sealing plate 410 and an outer pushing part 460.

[0044] Specifically, the tube replacement mechanism 400 further includes four telescopic rods 420 clamped and fixed at the corners of the left side wall of the movable sealing plate 410, a first spring 430 sleeved outside the telescopic rods 420, a second electric push rod 440 fixedly connected to the outer wall of the movable sealing plate 410 by screws, and a plug-in frame 450 clamped and fixed at the telescopic end of the second electric push rod 440.

[0045] Further, a plate surface chute 412 is formed on the outer wall of the movable sealing plate 410 and penetrates through from left to right to allow the plug-in frame 450 to move up and down. The other end of the telescopic rod 420 is clamped and fixed to the inner wall of the cabin body 110.

[0046] Further, the elastic force provided by the first spring 430 can push the movable sealing plate 410 to automatically reset during the rightward movement of the transverse movement frame 340, and the telescopic rod 420 is used to limit the contraction range of the first spring 430. This setting is to complete the automatic installation of the collection tube 240 and the recycling of the waste tube. By controlling the contraction of the telescopic rod of the second electric push rod 440, the plug-in frame 450 is driven to move smoothly, so that the collection tube 240 in the placement table 200 can be sleeved outside the sleeve tube 380, and the collection tube 240 outside the sleeve tube 380 can be pushed into the lower collection box 130.

[0047] Please refer to Figures 12-13 As shown, in this embodiment, the outer pushing part 460 includes an outer pushing frame 461 and an upper inserting block 462 whose top end extends into the dialing groove 211. A guiding groove 4610 is formed on the top surface of the outer pushing frame 461, and a lower convex rod 411 that slides inside the guiding groove 4610 is provided on the bottom surface of the movable sealing plate 410.

[0048] Specifically, the outer pushing part 460 further includes a plurality of second springs 463 welded to the bottom surface of the upper inserting block 462 and an outer connecting frame 464 clamped and fixed to the outer wall of the upper inserting block 462. The outer connecting frame 464 is slidably connected to the inside of the through groove 112.

[0049] Further, the outer pushing frame 461 is slidably connected to a cavity formed inside the cabin body 110. A groove 4611 is formed on the top surface of the horizontal plate at the right end of the outer pushing frame 461. The bottom end of the second spring 463 is welded and fixed to the bottom surface of the groove wall of the groove 4611. The top end cross section of the upper inserting block 462 is in the shape of a right trapezoid and the inclined end faces forward.

[0050] Further, when the movable sealing plate 410 moves leftward, the lower convex rod 411 contacts and moves along the groove wall of the guiding groove 4610. When driving the outer pushing part 460 to move backward, the upper inserting block 462 abuts against the dialing groove 211 on the bottom surface of the transverse movement table plate 210, and drives the transverse movement table plate 210 to move backward at a fixed distance, so that the next row of sampling holes of the microplate 220 are accurately aligned with the axis of the transverse movement frame 340, realizing the hole position switching of the sampling holes.

[0051] Further, the elastic force provided by the second spring 463 is such that when the upper insertion block 462 moves forward with the outer push frame 461, after coming into contact with the dial groove 211, it can contract into the interior of the groove 4611, and when it moves to below the next dial groove 211, it pops out under the elastic force of the second spring 463, preparing for the next fixed-distance backward movement of the transverse moving platen 210.

[0052] Finally, it should be noted that the motor 310, the first electric push rod 360, and the second electric push rod 440 involved in the present invention are all general standard parts or components known to those skilled in the art. Their structures and principles can all be learned by those skilled in the art through technical manuals or obtained through conventional experimental methods. At the idle places of the present device, the motor 310, the first electric push rod 360, and the second electric push rod 440 are connected to an external power supply through wires. The specific connection means should refer to the working principle of the present invention, and the electrical components are electrically connected in accordance with the sequence of their successive operations. Their detailed connection means are all well-known technologies in the art.

[0053] When the sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay of the present invention is in use, the operator first opens the hatch door 150 of the sampling chamber 100, accurately places a number of centrifuged blood collection tubes 230 in the designated openings on the right side plate surface of the transverse moving platen 210, then, successively places the corresponding collection tubes 240 in the openings on the left side plate surface of the transverse moving platen 210, and finally, steadily places the microplate 220 for holding the serum sample at the central position on the top surface of the transverse moving platen 210; After the placement of the samples and consumables is completed, the operator presses down the external connection frame 464 of the outer push part 460, so that the upper insertion block 462 smoothly disengages from the dial groove 211 opened on the bottom surface of the transverse moving platen 210. Subsequently, the entire transverse moving platen 210 carrying the blood collection tubes 230, the collection tubes 240, and the microplate 220 is pushed deep into the interior of the cabin body 110 of the sampling chamber 100. After confirming that the position is correct, the hatch door 150 is firmly closed to ensure that the internal environment of the cabin body 110 reaches a completely sealed state, providing stable conditions for subsequent automated operations; Subsequently, the motor 310 located inside the cabin is started. The motor 310 drives the slide plate 330 to start moving to the right within the track of the transverse moving frame 340. During this process, the convex rod 331 fixed on the slide plate 330 slides along the limit groove 341 on the transverse moving frame 340. When the convex rod 331 contacts the groove wall of the frame surface inclined groove 352 on the side surface of the docking frame 350, as the slide plate 330 continues to move to the right, the contact position of the convex rod 331 within the frame surface inclined groove 352 changes, and under geometric constraints, it forces the docking frame 350 to generate a downward displacement. After the downward movement of the docking frame 350 is completed, the slide plate 330 immediately pulls the entire transverse moving frame 340 to move synchronously to the right; When the transverse movement frame 340 is accurately moved to a predetermined position directly above the array of collection tubes 240 under the drive of the motor 310, the motor 310 is turned off. Immediately afterwards, the second electric push rod 440 is started. The telescopic rod of the second electric push rod 440 extends upwards, driving the plug-in frame 450 at its top and the collection tube 240 carried inside the plug-in frame 450 to be lifted upwards together. The collection tube 240 is lifted to a predetermined height, and its tube opening just inserts outside the sleeve 380 fixed above, preparing for subsequent serum collection; Subsequently, the motor 310 is started again to drive the transverse movement frame 340 to continue moving to the right. After the transverse movement frame 340 moves the plug-in frame 450 and the collection tube 240 away from their original positions, the second electric push rod 440 is controlled to retract, driving the plug-in frame 450 to descend smoothly and reset. The transverse movement frame 340 continues to move to the right until it reaches directly above the array of blood collection tubes 230. During this precise positioning process, the insertion rod 351 fixed on the docking frame 350 accurately inserts into the jack 142 opened at the top end of the lifting frame 140; Then, the motor 310 is controlled to reverse. The reverse drive of the motor 310 causes the slide plate 330 to start moving to the left inside the transverse movement frame 340. The convex rod 331 on the slide plate 330 interacts with the wall of the frame surface inclined groove 352 of the docking frame 350 again during the leftward movement. Due to the specific angle design of the frame surface inclined groove 352, the movement of the convex rod 331 forces the docking frame 350 to generate an upward displacement. Since the insertion rod 351 has been inserted into the jack 142 of the lifting frame 140, the upward movement of the docking frame 350 directly pulls the lifting frame 140 and the blood collection tube 230 fixed below it to rise together against gravity. The rising blood collection tube 230 accurately inserts outside the needle hole interface at the bottom end of the collection tube 240; At the same time, the telescopic end of the first electric push rod 360 is retracted, pulling the piston plate 370 connected to it to move upwards inside the collection tube 240, forming a negative pressure, thereby safely and quantitatively sucking the serum sample in the blood collection tube 230 into the cavity inside the collection tube 240; After the serum extraction is completed, the motor 310 is controlled to continue driving the slide plate 330 and the transverse movement frame 340 as a whole to move to the left. When the insertion rod 351 completely exits the jack 142 of the lifting frame 140 as the transverse movement frame 340 moves to the left, the lifting frame 140 and the blood collection tube 230 below it that have lost the connection automatically descend smoothly under their own gravity and return to the initial position; Under the precise control of the motor 310, the lateral frame 340 carrying the collection tube 240 from which the serum has been extracted begins to intermittently move to the left. Whenever the lateral frame 340 moves to the top of a sampling hole to be filled on the microplate 220, the motor 310 will pause to make the lateral frame 340 stay for a short time. During each stop, the first electric push rod 360 pushes the piston plate 370 downward to apply a slight pressure to the serum in the collection tube 240, so that a certain amount of serum droplets drip from the outlet of the collection tube 240 and drip accurately into the corresponding sampling hole of the microplate 220 below, completing the sample dispensing. After completing the dripping of the serum in the current row, the transverse frame 340 continues to move to the left driven by the motor 310. During this process, the left side of the transverse frame 340 contacts the movable sealing plate 410 and pushes the movable sealing plate 410 to move to the left together. Finally, the transverse frame 340 moves to the top of the collection box 130 located at a specific position inside the cabin 110. At this time, the second electric push rod 440 is controlled to drive the plug-in frame 450 to move downward. When the plug-in frame 450 moves down to the lowest point, the used collection tube 240 inside it is released and falls into the collection box 130 below under the action of gravity or slight pushing, completing the recovery of the waste tube; It is particularly critical that, when the movable sealing plate 410 is pushed to the left, the lower protruding rod 411 fixed thereon contacts and slides relatively with the guide groove 4610 designed on the push-out frame 461 of the push-out portion 460, and this sliding contact forces the entire push-out portion 460 assembly to move backward by a certain distance; When the pusher 460 moves backward, the upper insert block 462 at its upper end comes into conflict with the wall of the shifting groove 211 on the bottom surface of the transverse moving platen 210. The thrust generated by the conflict accurately shifts the entire transverse moving platen 210 backward by a fixed distance, so that the next row of sampling holes on the microplate 220 that have not yet been sampled automatically moves to the working position and is accurately aligned with the moving axis of the transverse moving frame 340, so as to prepare for the next round of operation. Subsequent operations completely repeat the above steps. With the coordinated cooperation of the motor 310, the first electric push rod 360 and the second electric push rod 440, the system automatically completes the transfer of serum samples in the blood collection tubes 230 placed on the transverse platform 210, the injection into the microplate 220 and the waste tube recovery until all samples are processed.

[0054] The foregoing description of specific exemplary embodiments of the invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that, according to the above teaching, many changes and variations are possible. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention, as well as various different selections and changes. The scope of the invention is intended to be defined by the specification and its equivalents.

Claims

1. Sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay, characterized in that: It includes a sampling chamber, a placement table inside it, a driving device arranged above the placement table, and a tube-changing mechanism on the left side of the placement table. The sampling chamber includes a lifting frame, and a jack is provided on the inner wall of its top end; The placement table includes a transverse moving table board, and a number of dial grooves are provided on the bottom surface of the transverse moving table board; The driving device includes a sliding plate, a transverse moving frame sleeved outside it, and a docking frame parallel to the sliding plate. An outer convex rod is provided on the outer wall of the sliding plate, and an insertion rod is provided on the right wall of the docking frame. A frame surface inclined groove for the outer convex rod to slide is provided on its rear wall. When the transverse moving frame moves to the rightmost side, the insertion rod is inserted into the jack. At this time, the sliding plate moves leftward, the outer convex rod abuts against the frame surface inclined groove, and the insertion rod immediately drives the lifting frame to rise; The tube-changing mechanism includes a movable sealing plate and an outer pushing part. The outer pushing part includes an outer pushing frame and an upper insertion block whose top end extends into the dial groove. A guiding groove is provided on the top surface of the outer pushing frame. A lower convex rod that slides inside the guiding groove is provided on the bottom surface of the movable sealing plate. When the movable sealing plate moves leftward, the lower convex rod contacts and moves along the groove wall of the guiding groove. When driving the outer pushing part to move backward, the upper insertion block abuts against the dial groove on the bottom surface of the transverse moving table board, and drives the transverse moving table board to move backward at a fixed distance, so that the next row of sampling holes of the microplate is accurately aligned with the axis of the transverse moving frame, realizing the hole position switching of the sampling holes.

2. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 1, characterized in that: The sampling chamber further includes a cabin body with an open rear end, two sliding rods clamped and fixed on the cabin walls at the left and right ends of the cabin body, a collection box slidably connected to the bottom of the cabin body, and a cabin door hinged at the open rear end of the cabin body.

3. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 2, characterized in that: A sliding groove for the lifting frame to move up and down is provided on the right side wall of the cabin body, a cavity for placing the tube-changing mechanism is provided inside the cabin body, and a round hole is provided on the bottom transverse board of the lifting frame.

4. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 1, characterized in that: The placement table further includes a microplate placed on the top surface of the transverse moving table board, and blood collection tubes and collection tubes arranged in an array on its left and right sides. The transverse moving table board is slidably connected to the inside of the cabin body. The distance between several dial grooves on the bottom surface of the transverse moving table board is the same as the distance between adjacent sampling holes on the microplate. A round rod for restricting the position of the microplate is integrally formed on the top surface of the transverse moving table board. The blood collection tubes and collection tubes are both slidably connected to the holes on the transverse boards on both sides of the transverse moving table board. When the lifting frame rises, the corresponding blood collection tubes are driven to rise together.

5. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 1, characterized in that: The driving device further includes a motor fixedly connected to the outer wall of the cabin body by screws, a lead screw coaxially connected to the output shaft of the motor, a first electric push rod fixedly connected to the top surface of the transverse moving frame by screws, a piston plate clamped to the telescopic end of the first electric push rod, and a sleeve sleeved outside the piston plate. The sleeve is clamped and fixed to the bottom surface of the transverse moving frame. The sleeve is used to clamp the collection tube. After the blood collection tube rises with the lifting frame, the needle port at the bottom end of the collection tube is inserted into the serum inside the blood collection tube, and the first electric push rod drives the piston plate to move upward to complete the serum extraction.

6. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 5, characterized in that: The sliding plate is threadedly connected to the lead screw. The outer convex rod is integrally formed with the sliding plate. A partition is integrally formed inside the transverse moving frame, and a limiting groove for restricting the moving range of the outer convex rod is provided on the partition. The transverse moving frame is slidably connected to the outside of the two sliding rods, and the docking frame is slidably connected to the inside of the transverse moving frame.

7. The sampling device for allergen-specific IgE antibodies based on chemiluminescence immunoassay according to claim 1, characterized in that: The tube replacement mechanism further includes four telescopic rods clamped and fixed at the corners of the left side wall of the movable sealing plate, a first spring sleeved outside the telescopic rods, a second electric push rod fixedly connected to the outer wall of the movable sealing plate by screws, and a plug-in frame clamped and fixed at the telescopic end of the second electric push rod.

8. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 7, characterized in that: A plate surface chute penetrating left and right for the up and down displacement of the plug-in frame is formed on the outer wall of the movable sealing plate, and the other end of the telescopic rod is clamped and fixed on the inner wall of the cabin.

9. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 1, characterized in that: The outer pushing part further includes a plurality of second springs welded to the bottom surface of the upper plug block and an external connection frame clamped and fixed to the outer wall of the upper plug block, and the external connection frame is slidably connected to the inside of the through groove.

10. The sampling device for allergen-specific IgE antibody based on chemiluminescence immunoassay according to claim 9, characterized in that: The outer pushing frame is slidably connected to a cavity formed inside the cabin. A groove is formed on the top surface of the horizontal plate at the right end of the outer pushing frame, and the bottom end of the second spring is welded and fixed to the bottom surface of the groove wall of the groove. The top cross section of the upper plug block is in the shape of a right trapezoid, and the inclined end faces forward.

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