Method for promoting rapid germination of Shatian pomelo seeds

Abstract

The invention discloses a method for promoting rapid germination of Shatian pomelo seeds. According to the method, fresh Shatian pomelo seeds and nutrient soil with the moisture content of 65% serve as a matrix, the seeds are sown at the position 0.5-1.5 cm below the surface layer of the nutrient soil, a potassium nitrate solution with the concentration of 1 g / L is sprayed to the surface of the nutrient soil, a seedling raising container is sealed and covered with a porous preservative film, then cultivation is conducted under the dark condition for 5-10 days, and seedlings with the germinated Shatian pomelo seeds are obtained. By the adoption of the method, the germination rate of the Shatian pomelo seeds can be remarkably increased to 90%, and the time needed by germination is shortened to 7 days; efficient germination of the Shatian pomelo seeds can be realized on the premise of not depending on hormone application and illumination equipment, and mildew and necrosis of the fresh seeds in the germination process cannot be caused. The method has the advantages of being easy and convenient to operate, low in cost and high in practicability, and is suitable for large-scale Shatian pomelo seedling breeding, popularization and application.

Description

Technical Field

[0001] The invention belongs to the technical field of fruit plant breeding and seed treatment, and particularly relates to a method for promoting rapid germination of pomelo seeds. Background Art

[0002] Pomelo Citrus maxima 'Shatian Yu' is a renowned high-quality local pomelo variety in my country, primarily grown in Shatian Village, Rong County, Guangxi Zhuang Autonomous Region, hence its name. It is also known as "Rongxian Shatian Pomelo," "White Heart Pomelo," and "Southern Honey Pomelo." The Shatian Yu boasts a well-shaped fruit with a smooth golden peel and a light yellow, transparent, tender, juicy flesh. Its flavor is sweet, slightly sour, and disintegrates easily, making it a highly sought-after high-quality pomelo variety in my country and even Southeast Asia. Rich in vitamin C, potassium, calcium, dietary fiber, and various natural antioxidants, Shatian Yu boasts health benefits such as boosting immunity, lowering blood lipids, promoting digestion, and clearing heat and reducing phlegm. The flesh can be eaten fresh or processed into a variety of foods, including juice, preserved fruit, and jam. The peel of the Shatian Yu has significant medicinal value. In Traditional Chinese Medicine, it is often used to regulate qi and relieve coughs, strengthen the spleen, and stimulate appetite. Essential oils can also be extracted for use in cosmetics and the flavor and fragrance industry. The seeds, peel, and even leaves of the Shatian Yu are also being used to develop plant extracts and feed additives, demonstrating significant potential for development. The elegant appearance of the pomelo tree and its fragrant flowers also offer considerable ornamental and ecological value. In recent years, the pomelo, a representative specialty agricultural product of the Guangxi Zhuang Autonomous Region, has become a major export product, possessing exceptional economic, nutritional, medicinal, and ecological value.

[0003] The Shatian pomelo is a subtropical evergreen fruit tree that prefers a warm, humid climate, ideally growing in areas with an average annual temperature of 20°C to 25°C and annual precipitation of at least 1,000 mm. It is moderately drought-tolerant but not waterlogged, requiring well-drained, slightly acidic sandy loam. Shatian pomelo prefers ample sunlight; insufficient sunlight can lead to reduced fruit quality. Its shallow root system necessitates shallow planting and careful water and fertilizer management. Water and nutrient requirements are particularly high during the flowering and fruiting phases, making it suitable for cultivation in low hilly areas or river valleys in southern China.

[0004] As one of the most important high-quality pomelo varieties in southern my country, Shatian pomelo possesses high economic value and promising prospects for widespread adoption. However, under conventional conditions, its seeds currently suffer from slow germination, uneven seedling emergence, and low emergence rates, severely hindering the rapid propagation and industrialization of seedlings. Therefore, exploring and establishing a scientific and efficient method for rapid seed germination of Shatian pomelo is of great research significance. On the one hand, this method could accelerate seedling production, improve efficiency and seedling quality, and meet the needs of large-scale planting and promotion. On the other hand, it would facilitate fruit tree breeding, resource selection, and the stable supply of subsequent experimental materials, providing a solid foundation for the selection and regionalization of Shatian pomelo varieties, and positively promoting the development of the local fruit industry and the revitalization of the rural economy. Summary of the Invention

[0005] The object of the present invention is to solve the problems of long germination cycle, uneven germination, low emergence rate, and weak growth of seedlings of existing Shatian pomelo seeds under natural conditions, and to provide a method that is simple to operate, efficient, highly repeatable, and suitable for the rapid germination of Shatian pomelo seeds. In existing methods, the germination of Shatian pomelo seeds is affected by various factors, such as seed freshness, uneven moisture in the germination substrate, improper light conditions, unreasonable covering methods, improper treatment with exogenous substances, etc., resulting in low germination rate and too long seedling raising cycle, making it difficult to meet the requirements of efficient seedling raising and large-scale production. Therefore, it is urgent to establish a scientific, systematic, and highly controllable germination scheme to improve the germination rate and uniformity of Shatian pomelo seeds, and provide technical support for subsequent seedling raising and popularization and planting.

[0006] The present invention starts from key factors such as seed type, substrate type, covering method, and application of exogenous substances. Through experiments, the optimal treatment in each factor is screened out in turn, and then combined to obtain a germination method that can shorten the germination time of Shatian pomelo seeds, improve the germination rate and the quality of initial growth.

[0007] A method for promoting the rapid germination of Shatian pomelo seeds of the present invention includes the following steps: S1. Remove the seed coat of fresh Shatian pomelo (Citrus maxima 'Shatian Yu') seeds, wash them clean with water, then soak them in water for 1 day, leave the viable seeds to continue soaking for 2 days, and then take out the seeds for standby; S2. Prepare nutrient soil with a moisture content of 65%, fill the nutrient soil into a seedling raising container, evenly bury the seeds prepared in step S1 under the surface of the nutrient soil to a depth of 0.5 - 1.5 cm, spray a 1 g / L potassium nitrate solution on the surface of the nutrient soil, seal and cover the seedling raising container with a perforated plastic film, and then cultivate in the dark for 5 - 10 days to obtain seedlings of germinated Shatian pomelo seeds.

[0008] Preferably, the viable seeds left are the seeds that sink to the bottom after removing the floating seeds.

[0009] Preferably, during the soaking, change the water once a day.

[0010] Preferably, the seedling raising container is a disposable plastic lunch box made of PP polypropylene with a specification of 5 cm in height, 10 cm in width, and 20 cm in length.

[0011] Preferably, the nutrient soil is the Substrate nutrient soil product of the PINDSTRUP brand.

[0012] Preferably, the perforated fresh-keeping film is prepared by punching the fresh-keeping film with a bamboo stick. The diameter of the bamboo stick is 0.2 cm, and the punching density is 20 - 25 holes per 10 cm².

[0013] Preferably, the application amount of the 1 g / L potassium nitrate solution sprayed is 125 mL of the 1 g / L potassium nitrate solution per square meter (i.e., 2.5 mL is sprayed on an area of 10 cm × 20 cm).

[0014] Preferably, the cultivation under dark conditions is carried out for 7 days under dark conditions.

[0015] Advantages of the present invention: By using the method of the present invention, the germination rate of Shatian pomelo seeds can be significantly increased to 90%, and the time required for germination can be shortened to 7 days. At the same time, the present invention can achieve efficient germination of Shatian pomelo seeds without relying on hormone application and lighting equipment, and will not cause mildew and necrosis of fresh seeds during germination. The method of the present invention has the characteristics of simple operation, low cost, and strong practicability, and is suitable for large-scale breeding and popularization of Shatian pomelo seedlings. Description of the drawings

[0016] Figure 1 It is a result diagram of the germination of fresh Shatian pomelo seeds in Guangxi Zhuang Autonomous Region for 10 days with different substrate types (selecting 5 seeds with the middle root length in each group for display); among them, A: nutrient soil (water content 65%); B: sphagnum moss (water content 80%); C: quartz sand (water content 10%); D: filter paper (adding 25 mL of water); scale = 2 cm.

[0017] Figure 2 It is a result diagram of the germination of fresh Shatian pomelo seeds in Guangxi Zhuang Autonomous Region for 7 days with different covers (selecting 5 seeds with the middle root length in each group for display); among them, A: perforated film (at least 20 holes are poked with a bamboo stick per 10 cm²); B: lunch box lid; C: non-perforated film; D: no lid; scale = 2 cm.

[0018] Figure 3 It is a result diagram of the germination of fresh Shatian pomelo seeds in Guangxi Zhuang Autonomous Region for 7 days under different light conditions (selecting 5 seeds with the middle root length in each group for display); among them, A: dark; B: light (light intensity 1800 lux, 12 hours per day); scale = 2 cm).

[0019] Figure 4It is a result graph of the first batch of different exogenous chemicals on the germination of fresh Shatian pomelo seeds in Guangxi Zhuang Autonomous Region for 7 days (selecting 5 seeds with the median root length in each group for display); among them, A: control group (without applying exogenous chemicals); B: activated carbon (2 g / L, applying 1.6 g); C: hydrogen peroxide (50 mmol / L, applying 2.5 mL); D: potassium nitrate (1 g / L, applying 2.5 mL); scale bar = 2 cm.

[0020] Figure 5 It is a result graph of the second batch of different exogenous chemicals on the germination of fresh Shatian pomelo seeds in Guangxi Zhuang Autonomous Region for 7 days (selecting 5 seeds with the median root length in each group for display); among them, A: control group (without applying exogenous chemicals); B: filter paper (adding 25 mL of water); C: lanthanum chloride (800 μmol / L, applying 2.5 mL); D: cobalt chloride (800 μmol / L, applying 2.5 mL); E: GA3 (0.01 μmol / L, applying 2.5 mL); F: IAA (0.1 μmol / L, applying 2.5 mL); G: potassium nitrate (1 g / L, applying 2.5 mL); scale bar = 2 cm.

[0021] Figure 6 It is a result graph of the hydroponics of Shatian pomelo seedlings with the optimal germination combination and the worst germination combination for 10 days (selecting 5 plants with the median plant height in each group for display); among them, A: potassium nitrate (1 g / L potassium nitrate in the germination stage, applying 2.5 mL); B: filter paper (adding 25 mL of water to the filter paper in the germination stage); scale bar = 2 cm. Specific implementation manners

[0022] The following embodiments are further descriptions of the present invention, rather than limitations on the present invention.

[0023] The experimental route is designed as follows: In the first stage, select dry Shatian pomelo seeds in Guangxi Zhuang Autonomous Region as materials. After removing the hard outer seed coat, wash them clean with deionized water, and then soak them in deionized water for 1 day. Select the viable seeds that sink to the bottom and soak them for another 2 days, changing the water once a day during this period, and then the treatment materials for the germination experiment can be obtained. Subsequently, the seeds after soaking for 3 days are respectively inoculated into 200 mm diameter petri dishes filled with nutrient soil (water content 65%), yellow sand (water content 10%) and filter paper (adding 25 mL of water), and uniformly germinated under the condition of a temperature of 25°C. The experiment in the first stage only sets one variable of the substrate type.

[0024] In the second stage, fresh seeds of Shatian pomelo from Guangxi Zhuang Autonomous Region were selected. After removing the hard outer seed coat, they were washed clean with deionized water, then soaked in deionized water for 1 day. The floating seeds were removed, and the viable seeds that sank to the bottom were left to soak for another 2 days. The total soaking time was 3 days, with the water changed once a day, and the treatment materials for the germination experiment could be obtained. Subsequently, the seeds after soaking for 3 days were inoculated into disposable plastic lunch boxes with a specification of 5 cm (height) × 10 cm (width) × 20 cm (length) filled with substrate, and different experimental conditions were applied. They were uniformly germinated in a constant temperature environment at 25°C. In the experiment, with "root length greater than 0.5 cm" as the germination determination criterion, the germination rate of each group of seeds was statistically analyzed. Four variables were set in the second-stage experiment, namely: substrate type, covering method, light treatment, and addition of exogenous substances. Single-factor comparative experiments were carried out in sequence to screen out the representative optimized combinations that promote the rapid germination of fresh Shatian pomelo seeds.

[0025] Among them, the disposable plastic lunch boxes used were those with a specification of 5 cm in height, 10 cm in width, and 20 cm in length, made of PP polypropylene. The nutrient soil used was the Substrate nutrient soil product of the PINDSTRUP brand. The potassium nitrate used was an analytical reagent provided by Guangzhou Chemical Reagent Factory. The plastic wrap used was ordinary household food-grade PE plastic wrap.

[0026] Example 1 Effect of different substrate types on the germination of dry Shatian pomelo seeds from Guangxi Zhuang Autonomous Region To explore the effect of different substrate types on the germination of dry Shatian pomelo seeds from Guangxi Zhuang Autonomous Region, three treatments were set in this experiment: nutrient soil (water content 65%, seeds evenly buried 0.5 - 1.5 cm below the surface of the nutrient soil), yellow sand (water content 10%, seeds evenly buried 0.5 - 1.5 cm below the surface of the yellow sand), and filter paper (25 mL of water added, seeds evenly placed on the filter paper). Each treatment group was inoculated with dry Shatian pomelo seeds (70 seeds after shelling, seed viability screening, and soaking for 3 days) as raw materials. Each group was uniformly covered with perforated plastic wrap and cultured in the dark at 25°C for 30 days. Water was replenished once every 10 days, and the germination rate was statistically analyzed once. This was repeated 3 times.

[0027] Table 1 Effect of different substrate types on the germination rate and seedling root length of dry Shatian pomelo seeds (germination for 30 days) The results (Table 1) showed that when using dry seeds as raw materials and germinating them in different substrates, the germination rate was extremely low within 30 days, and the highest germination rate was only 1.4% (in the nutrient soil group). At the same time, the experiment found that when using dry seeds as raw materials, after 20 days of germination, the Shatian pomelo seeds in the yellow sand group and the filter paper group began to rot and stink, indicating that some seeds had started to die. Therefore, in subsequent experiments, we chose fresh Shatian pomelo seeds as raw materials to conduct the experiments.

[0028] Example 2: Effects of Different Substrate Types on the Germination of Fresh Shatian Pomelo Seeds from Guangxi Zhuang Autonomous Region To explore the effects of different substrate types on the germination of fresh Shatian pomelo seeds from Guangxi Zhuang Autonomous Region, this experiment set up 4 treatments: nutrient soil (with a water content of 65%, seeds evenly buried 0.5 - 1.5 cm deep under the surface of the nutrient soil), sphagnum moss (with a water content of 80%, seeds evenly buried 0.5 - 1.5 cm deep under the surface of the sphagnum moss), quartz sand (with a water content of 10%, seeds evenly buried 0.5 - 1.5 cm deep under the surface of the quartz sand), and filter paper (added with 25 mL of water, seeds evenly placed on the filter paper). Each treatment group was inoculated with fresh Shatian pomelo seeds (21 seeds after shelling, seed vigor screening, and soaking in water for 3 days) as raw materials. Each group was uniformly covered with perforated plastic wrap and cultured under dark conditions at 25°C for 10 days, and then the seed germination rate and the average root length of the seedlings were measured. The experiment was repeated 3 times.

[0029] Table 2: Effects of Different Substrate Types on the Germination Rate and Seedling Root Length of Fresh Shatian Pomelo Seeds (Germination for 10 Days) Note: Different letters after the data indicate significant differences between the data (p < 0.05).

[0030] As can be seen from Table 2 and Figure 1 It can be known that the germination rate of the nutrient soil group was 100%, and the average root length of the seedlings reached 4.26 cm, which was significantly higher than that of other substrate groups (p < 0.05), showing the best germination effect among all treatments. The germination rates of the sphagnum moss and quartz sand groups were both about 80%, and the root lengths were slightly lower; the germination rate of the filter paper group was the lowest, only 10%, and the root length was significantly the shortest, only 0.59 cm. After comprehensive comparison, nutrient soil showed the best performance in promoting the germination of fresh Shatian pomelo seeds and was the optimal germination substrate. Since the effect of the nutrient soil group was extremely good after 10 days of germination in this experiment, with a germination rate as high as 100%, all subsequent experiments were carried out with nutrient soil as the substrate, and at the same time, the germination cycle was shortened to 7 days.

[0031] Example 3: Effects of Different Covers on the Germination of Fresh Shatian Pomelo Seeds from Guangxi Zhuang Autonomous Region After determining that the nutrient soil was the most suitable substrate, to further explore the effect of covering methods on the germination of fresh Shatian pomelo seeds, four covering treatments were set up: punctured plastic wrap (perforated film: 20 - 25 holes were punctured in every 10 cm² of plastic wrap with a bamboo stick (diameter 0.2 cm)), lunch box lid (lunch box cover: the lid matching the germination container), non - perforated plastic wrap, and no lid. Each treatment group uniformly used nutrient soil (water content 65%) as the substrate, inoculated with fresh Shatian pomelo seeds (21 seeds after shelling, seed vigor screening, and soaking in water for 3 days) as raw materials. Under dark conditions at 25°C, after 7 days of cultivation, the germination rate of the seeds, the root length, fresh weight, and dry weight of the seedlings were measured. This was repeated 3 times.

[0032] Table 3 Effects of different covers on the germination rate, seedling root length, fresh weight, and dry weight of fresh Shatian pomelo seeds (germination for 7 days) Note: Different letters after the data indicate significant differences between the data (p < 0.05).

[0033] The results (Table 3, Figure 2 ) showed that the perforated film group and the lunch box lid group performed better in terms of germination rate, root length, and fresh weight. The germination rates were both about 70%, the root lengths were 2.32 cm and 2.34 cm respectively, with no significant difference (p > 0.05), and the fresh weight and dry weight were both at a relatively high level. The non - perforated film group was second, with the germination rate dropping to 45% and the root length significantly shortened. The no - lid group performed the worst, with a germination rate of 0% and all seeds having dried up. Considering the air permeability and mildew - proof effect of the covering materials comprehensively, under the same sealing efficiency, the perforated film group was finally selected as the optimal covering method during the germination stage of Shatian pomelo seeds.

[0034] Example 4 Effects of different light conditions on the germination of fresh Shatian pomelo seeds from Guangxi Zhuang Autonomous Region On the basis of the experimental screening in Examples 2 and 3, where "nutrient soil + perforated film" was selected as the better combination, to further explore the effect of light conditions on the germination of Shatian pomelo seeds, two treatment conditions were set up: dark cultivation and light cultivation. In the dark group, there was no light throughout the process; in the light group, the light cycle was 12 hours per day and the light intensity was 1800 lux. Each treatment group uniformly used nutrient soil (water content 65%) as the substrate, inoculated with fresh Shatian pomelo seeds (21 seeds after shelling, seed vigor screening, and soaking in water for 3 days) as raw materials, covered with perforated plastic wrap, and under the condition of 25°C, after 7 days of cultivation, the germination rate of the seeds, the root length, fresh weight, and dry weight of the seedlings were measured. This was repeated 3 times.

[0035] Table 4 Effects of different light conditions on the germination rate, seedling root length, fresh weight, and dry weight of fresh Shatian pomelo seeds (germination for 7 days) Note: The asterisk behind the data indicates a highly significant difference between the data (** p<0.01).

[0036] The results (Table 4, Figure 3 ) showed that light conditions were conducive to improving the germination rate and root length of Shatian pomelo seeds. Among them, the germination rate of the light treatment was 85%, higher than 70% of the dark treatment, and the root length of the seedlings was also better. However, the fresh weight and dry weight of the seedlings under dark conditions were significantly higher than those of the light group, indicating that appropriate shading was beneficial to initial nutrient accumulation. At the same time, considering the limited light resources in the actual seedling raising environment and the fact that the dark treatment could also achieve a relatively high germination rate and good growth performance, the dark condition was preferentially used as the treatment method for Shatian pomelo seed germination in the subsequent experiments of the present invention.

[0037] Example 5 Effects of Different Exogenous Chemical Substances on the Germination of Fresh Shatian Pomelo Seeds in Guangxi Zhuang Autonomous Region On the premise that "nutrient soil + darkness + perforated film" had been determined as the basic culture conditions in Examples 2-4, in order to explore the effects of different exogenous chemical substances on the germination of fresh Shatian pomelo seeds, the following 7 treatments were set up and divided into two batches of experiments. The first batch: 1 g / L potassium nitrate (2.5 mL), 50 mmol / L hydrogen peroxide (2.5 mL), 2 g / L activated carbon (1.6 g solid); the second batch: 800 μmol / L lanthanum chloride (2.5 mL), 800 μmol / L cobalt chloride (2.5 mL), 0.01 μmol / L GA3 (2.5 mL), 0.1 μmol / L IAA (2.5 mL). All were applied separately. A control group without applying exogenous chemical substances was set up in each batch of experiments. At the same time, in the second batch of experiments, a group with the highest germination rate in the first batch of experiments and a filter paper group with the lowest germination rate among fresh seeds were added for comparison. Except for the filter paper group, each of the other groups uniformly used nutrient soil (water content 65%) as the substrate. Each group was inoculated with 21 fresh Shatian pomelo seeds (after shelling, seed vigor screening and soaking in water for 3 days) as raw materials. The above-mentioned treatment solutions were evenly sprayed on the surface of the nutrient soil, covered with a perforated plastic film, and cultured under dark conditions at 25°C for 7 days, and then the relevant indicators were measured. No additional liquid was added during the treatment period. Repeat 3 times.

[0038] Table 5 Effects of the First Batch of Exogenous Chemical Substances on the Germination Rate and Seedling Root Length of Fresh Shatian Pomelo Seeds (Germination for 7 Days) Note: Different letters behind the data indicate a significant difference between the data (p<0.05).

[0039] Table 6 Effects of the Second Batch of Exogenous Chemical Substances on the Germination Rate and Seedling Root Length of Fresh Shatian Pomelo Seeds (Germination for 7 Days) Note: Different letters after the data indicate significant differences between the data (p < 0.05).

[0040] In the first batch of exogenous substance treatments, the germination rate of the potassium nitrate treatment group reached 90% (Table 5, Figure 4 ), which was the highest among all groups. The root length of the seedlings was also significantly better than that of the activated carbon, hydrogen peroxide, and control groups, and the overall performance was the best. In the second batch of treatments, the germination rate and seedling root length data of the potassium nitrate treatment group were still the highest (Table 6, Figure 5 ). The germination rates of the IAA, GA3, lanthanum chloride, and cobalt chloride groups were all lower than that of the control group. Based on the combined results of the two batches of experiments, it can be seen that an appropriate amount of potassium nitrate is the most significant in improving the germination rate and root growth of Shatian pomelo seeds, and it is the best-performing exogenous promoter.

[0041] Example 6 Effects of the optimal germination treatment combination and the worst combination on the hydroponic growth of Shatian pomelo seedlings To further verify the performance differences between the optimal germination treatment combination and the worst combination selected from Examples 2-5 using fresh Shatian pomelo seeds as raw materials in the subsequent seedling raising stage, we transferred the seedlings of the potassium nitrate group and the filter paper group after 7 days of germination in the second batch of experiments in Example 5 into black buckets and hydroponically cultured them for 10 days under the condition of not adding nutrient solution, and measured the plant height. The results are shown in Table 7 and Figure 6 as follows.

[0042] Table 7 Effects of the optimal germination treatment combination and the worst combination on the subsequent growth of Shatian pomelo seedlings Note: The asterisks after the data indicate extremely significant differences between the data (** p < 0.01).

[0043] The results show that the plant height of Shatian pomelo seedlings treated with 1 g / L potassium nitrate during the germination stage was significantly better than that of the filter paper group during the hydroponic stage, with strong growth potential and significant differences. In contrast, the data of the filter paper group were already low in terms of germination rate and seedling root length, and the subsequent growth was also inhibited. This result further verifies that the treatment method during the germination stage has a continuous impact on subsequent growth. Appropriate exogenous stimulation (such as applying potassium nitrate) can significantly improve seed vigor and promote seedling growth, providing an effective means for rapid seedling raising.

[0044] In summary, the present invention recommends using the combined scheme of "dark condition + nutrient soil with a water content of 65% + applying 2.5 mL of 1 g / L potassium nitrate solution + covering with a plastic wrap punctured with holes". This scheme has the characteristics of simple operation, low cost, and high efficiency, and is suitable for the actual popularization and application of Shatian pomelo seedling raising.

Claims

1. A method for promoting the rapid germination of Shatian pomelo seeds, characterized in that, Comprising the following steps: S1. Remove the seed coats of the fresh 'Shatian Yu' ( Citrus maxima 'Shatian Yu') seeds, wash them clean with water, then soak them in water for 1 day, leave the viable seeds and continue to soak them for another 2 days, change the water once a day during the soaking period, and then take out the seeds for standby; S2. Prepare nutrient soil with a moisture content of 65%, fill the nutrient soil into a seedling container, evenly bury the seeds prepared in step S1 at a depth of 0.5 - 1.5 cm below the surface of the nutrient soil, spray a 1 g / L potassium nitrate solution on the surface of the nutrient soil, seal and cover the seedling container with a perforated plastic film, and then cultivate it under dark conditions for 5 - 10 days to obtain seedlings germinated from Shatian pomelo seeds.

2. The method according to claim 1, characterized in that, The viable seeds left are the seeds that sink to the bottom after removing the floating seeds.

3. The method according to claim 1, wherein The seedling container is a disposable plastic lunch box made of PP polypropylene with a specification of 5 cm in height, 10 cm in width, and 20 cm in length.

4. The method according to claim 1, wherein The nutrient soil is the Substrate nutrient soil product of the PINDSTRUP brand.

5. The method according to claim 1, characterized in that The perforated plastic film is prepared by punching holes in the plastic film with a bamboo stick. The diameter of the bamboo stick is 0.2 cm, and the punching density is 20 - 25 holes per 10 cm².

6. The method according to claim 1, characterized in that, The application amount of the sprayed 1 g / L potassium nitrate solution is 125 mL of 1 g / L potassium nitrate solution per square meter.

7. The method according to claim 1, characterized in that, During the soaking, change the water once a day.

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