High-activity synbiotics nourishment as well as preparation method and application thereof

Abstract

The invention provides a high-activity synbiotics nourishment as well as a preparation method and application thereof, and relates to the technical field of activity excitation and protection of probiotics, prebiotics and metabiotics. The preparation method provided by the invention comprises the following steps: freeze-drying and crushing pomelo peel white capsules to obtain pomelo peel powder; stirring and dispersing the shaddock peel powder in ethanol, separating and drying to obtain modified powder; mixing the modified powder with a ferroferric oxide nano suspension, and separating to obtain a magnetic carrier; mixing and loading the magnetic carrier and the probiotic liquid in an alternating magnetic field, and separating to obtain a probiotic carrier; mixing and adsorbing the probiotic carrier in a sodium alginate solution, and separating to obtain a gel precursor; performing ultrasonic dispersion on the gel precursor in a calcium ion solution, and performing gel shaping to obtain the high-activity synbiotic nourishment. According to the invention, not only can the shaddock peel be subjected to resource utilization, but also the damage of gastrointestinal tracts to the probiotics can be reduced, the release time of the probiotics can be delayed, and the viable bacteria quantity of the probiotics reaching the colon can be increased, so that the physiological activity of the probiotics and the bioavailability of a human body can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of activating and protecting the activities of probiotics, prebiotics, and postbiotics, and particularly relates to a highly active synbiotic nutritional product, a preparation method thereof, and an application thereof. Background Art

[0002] Probiotics are a class of active microorganisms that can colonize the human intestine. Through mechanisms such as regulating the balance of the intestinal flora, enhancing the intestinal barrier function, and inhibiting the proliferation of pathogenic bacteria, they can improve human health in multiple dimensions. However, when probiotics are directly introduced, they are easily inactivated in the gastric juice environment. Therefore, in order to improve the bioavailability of probiotics, probiotics are usually activated and encapsulated to form a nutritional system to form a physical / chemical protection barrier for probiotics, which can effectively reduce the loss of probiotics caused by external environmental factors and effectively improve the stability of probiotics, thereby achieving the purpose of slow release in the human intestine. However, the existing nutritional systems are difficult to synergistically improve the release rate and continuous release time of probiotics, prebiotics, and postbiotics, and it is difficult to controllably transport a sufficient amount of probiotics, prebiotics, and postbiotics to the distal intestine. Therefore, there is an urgent need to provide a solution to improve the above problems. Summary of the Invention

[0003] The purpose of the present invention is to provide a highly active synbiotic nutritional product, a preparation method thereof, and an application thereof, which can reduce the damage of the gastrointestinal tract to probiotics, delay the release time of probiotics, increase the viable count of probiotics reaching the colon, and thus improve the physiological activity and human bioavailability of probiotics.

[0004] In a first aspect, a preparation method of a highly active synbiotic nutritional product provided by the present invention includes: freeze-drying and pulverizing the white pith of pomelo to obtain pomelo peel powder; stirring and dispersing the pomelo peel powder in ethanol and then separating and drying to obtain a modified powder; mixing the modified powder with a magnetic iron oxide nanosuspension and then separating to obtain a magnetic carrier; mixing the magnetic carrier with a probiotic bacterial solution in an alternating magnetic field for loading and then separating to obtain a probiotic carrier; mixing and adsorbing the probiotic carrier in a sodium alginate solution and then separating to obtain a gel precursor; ultrasonically dispersing the gel precursor in a calcium ion solution and gel-curing to obtain the highly active synbiotic nutritional product.

[0005] Optionally, the source of the white pith of pomelo includes one of Shatian pomelo, Wentan pomelo, red-fleshed honey pomelo, tangerine pomelo, Jinxiang pomelo, Pingshan pomelo, Jinlan pomelo, Anjiang fragrant pomelo, Liangping pomelo, and late white pomelo.

[0006] Optionally, the probiotics in the probiotic bacterial solution include one of Lactobacillus plantarum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium bifidum, Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus paracasei, Lactococcus lactis, and Streptococcus thermophilus.

[0007] Optionally, the pummelo albedo is freeze-dried, crushed, and sieved through a 40-mesh to 100-mesh sieve to obtain pummelo peel powder.

[0008] Optionally, the pummelo albedo is freeze-dried at -15°C to -20°C.

[0009] Optionally, the pummelo peel powder is freeze-dried for 1 h - 2 h.

[0010] Optionally, the pummelo peel powder is stirred and dispersed in ethanol and then subjected to ultrasonic treatment.

[0011] Optionally, the pummelo peel powder is stirred and dispersed in ethanol at 30°C - 50°C.

[0012] Optionally, the particle size of the iron oxide in the iron oxide nanosuspension is 10 nm - 50 nm.

[0013] Optionally, the modified powder and the iron oxide nanosuspension are mixed and then subjected to ultrasonic treatment.

[0014] Optionally, the mass ratio of the modified powder to the iron oxide in the iron oxide nanosuspension is 1:(0.1 - 0.2).

[0015] Optionally, the modified powder and the iron oxide nanosuspension are mixed for 10 h - 16 h.

[0016] Optionally, the magnetic carrier and the probiotic bacterial liquid are mixed in an alternating magnetic field of 0.3 mT - 0.4 mT.

[0017] Optionally, the mixing and loading in the alternating magnetic field is carried out for 6 h - 10 h.

[0018] Optionally, the probiotic carrier is obtained by magnetic separation after mixing and loading.

[0019] Optionally, the probiotic carrier is adsorbed in a sodium alginate solution with a concentration of 1% - 3%.

[0020] Optionally, the magnetic carrier is obtained by drying in vacuum at 60°C - 80°C for 2 h - 5 h after separation.

[0021] Optionally, the pummelo peel powder is stirred and dispersed in ethanol for 8 h - 15 h.

[0022] Optionally, the modified powder is obtained by drying in vacuum at 50°C - 70°C after separation.

[0023] Optionally, the calcium ion solution includes calcium chloride solution.

[0024] Optionally, ultrasonic dispersion is carried out in the calcium ion solution for 15 min - 30 min.

[0025] Optionally, when the magnetic carrier is mixed with the probiotic bacterial liquid in an alternating magnetic field, the probiotic bacterial powder is pre-mixed and incubated with the nano-ferroferric oxide physiological saline mixture, and after magnetic separation and removal of the supernatant, it is resuspended with physiological saline to obtain the probiotic bacterial liquid.

[0026] In a second aspect, the present invention also provides a highly active synbiotic nutritional product prepared by any of the above optional preparation methods.

[0027] Optionally, the loading amount of probiotics in the nutritional product is 1×10 9 CFUs - 2×10 9 CFUs.

[0028] Optionally, the release rate of the nutritional product is 1×10 8 CFUs / h - 2×10 8 CFUs / h.

[0029] Optionally, the release time of the nutritional product for probiotics is 6h - 10h.

[0030] In a third aspect, the present invention also provides an application of a highly active synbiotic nutritional product. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a flowchart of a preparation method of a highly active synbiotic nutritional product provided by the present invention;

[0032] Figure 2 is a comparison chart of the loading amount of Lactobacillus plantarum in the nutritional products prepared in Example 1, Example 2 and Comparative Example 1 of the present invention;

[0033] Figure 3 is a release curve diagram of Lactobacillus plantarum in the nutritional products prepared in Example 1, Example 2 and Comparative Example 1 of the present invention during simulated gastrointestinal digestion;

[0034] Figure 4 is a pH change curve diagram of the nutritional products prepared in Example 1, Example 2 and Comparative Example 1 of the present invention during human flora fermentation;

[0035] Figure 5 is a comparison chart of the change in the total short-chain fatty acid content of the nutritional products prepared in Example 1, Example 2 and Comparative Example 1 of the present invention during human flora fermentation. DETAILED DESCRIPTION OF THE INVENTION

[0036] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein shall have the ordinary meanings as understood by those of ordinary skill in the art to which the present invention pertains.

[0037] See Figure 1 , the present invention provides a method for preparing a highly active synbiotic nutrient, comprising the following steps:

[0038] S1. Freeze-dry and pulverize the white pith of pomelo peel to obtain pomelo peel powder;

[0039] S2. Stir and disperse the pomelo peel powder in ethanol, then separate and dry it to obtain modified powder;

[0040] S3. Mix the modified powder with a magnetic iron oxide nanoparticle suspension, then separate to obtain a magnetic carrier;

[0041] S4. Mix the magnetic carrier with probiotic bacteria liquid in an alternating magnetic field for loading, then separate to obtain a probiotic carrier;

[0042] S5. Mix and adsorb the probiotic carrier in a sodium alginate solution, then separate to obtain a gel precursor;

[0043] S6. Ultrasonically disperse the gel precursor in a calcium ion solution and gel it to obtain a highly active synbiotic nutrient.

[0044] In fact, pomelo peel, as an agricultural by-product, is usually directly discarded or dried and used as traditional Chinese medicine, without more ways of resource utilization. By modifying the white pith of pomelo peel to increase surface pores and surface activity, it is beneficial to improve the adsorption of magnetic iron oxide nanoparticles, thereby increasing the loading amount of probiotics and forming gel beads as gel nuclei. It can not only utilize pomelo peel resources, but also reduce the damage of the gastrointestinal tract to probiotics, delay the release time of probiotics, increase the viable count of probiotics reaching the colon, and thus improve the physiological activity and human bioavailability of probiotics.

[0045] In some embodiments, the source of the white pith of pomelo peel used in performing step S1 includes one of Shatian pomelo, Wentan pomelo, red-fleshed honey pomelo, tangerine pomelo, Jinxiang pomelo, Pingshan pomelo, Jinlan pomelo, Anjiang fragrant pomelo, Liangping pomelo, and late white pomelo. In fact, when using the white pith of pomelo peel, the surface of the pomelo peel with fresh pomelo flesh removed can be removed, and the white pith part can be sliced for freeze-drying and pulverization.

[0046] In some embodiments, when performing step S1, the white pith of pomelo peel can be freeze-dried at -15°C to -20°C for 1h - 2h, which can effectively increase the porous pores on the surface of the white pith and reduce the water content, facilitating further improvement of the specific surface area of the pomelo peel powder, and then increasing the loading amount of probiotics and magnetic iron tetroxide. Specifically, after the white pith of pomelo peel is completed with freeze-drying treatment, it can be ground and pulverized and passed through a 40-mesh to 100-mesh sieve to obtain micron-sized pomelo peel powder.

[0047] In some embodiments, when performing step S2, the pomelo peel powder can be stirred and dispersed in ethanol at 30°C - 50°C and then ultrasonic-treated. This is beneficial to improving the surface modification efficiency of ethanol on the pomelo peel powder. At the same time, through ultrasonic treatment, it can promote the uniform dispersion of the pomelo peel powder in ethanol, which can not only prevent the sedimentation of the pomelo peel powder, but also utilize the ultrasonic cavitation effect to remove the tiny bubbles on the surface, further improving the surface modification efficiency and uniformity.

[0048] In some embodiments, the particle size of iron tetroxide in the iron tetroxide nano-suspension used when performing step S3 is 10nm - 50nm. In fact, by blending the modified powder with the iron tetroxide nano-suspension, it is beneficial to adsorb iron tetroxide nano-particles inside the modified powder, thereby magnetically modifying the modified powder. Specifically, in step S3, the mass ratio of the modified powder to iron tetroxide in the iron tetroxide nano-suspension is 1:(0.1 - 0.2), and ultrasonic treatment can be performed after mixing. In some embodiments, the modified powder and the iron tetroxide nano-suspension are mixed for 10h - 16h in step S3.

[0049] In some embodiments, when performing step S4, the magnetic carrier and the probiotic bacterial liquid are mixed and loaded in an alternating magnetic field of 0.3mT - 0.4mT for 6h - 10h, and the probiotic carrier is obtained by magnetic separation after mixing and loading. Specifically, when the magnetic carrier and the probiotic bacterial liquid are mixed in the alternating magnetic field, the probiotic bacterial powder is pre-mixed and incubated with the nano-iron tetroxide normal saline mixture, and after magnetic separation and removal of the supernatant, it is resuspended with normal saline to obtain the probiotic bacterial liquid.

[0050] In some embodiments, when performing step S5, the probiotic carrier is adsorbed in a sodium alginate solution with a concentration of 1% - 3%. In step S6, the calcium ion solution includes calcium chloride solution, and it is ultrasonically dispersed in the calcium ion solution for 15min - 30min.

[0051] The present invention also provides a highly active synbiotic nutritional product prepared by using any of the above optional preparation methods. The loading amount of probiotics in the nutritional product is 1×10 9 CFUs - 2×10 9 CFUs, and the release rate is 1×10 8 CFUs / h - 2×108 CFUs / h, and the release time of the probiotics is 6 h - 10 h.

[0052] Example 1

[0053] Example 1 provides a method for preparing a highly active synbiotic nutrient, comprising the following steps:

[0054] S1. After removing the epidermis of the pomelo peel of Guangfeng Pomelo (red-fleshed pomelo) to obtain the white sac, freeze-dry it at -18 °C for 2 h and then crush it through a 100-mesh sieve to obtain pomelo peel powder;

[0055] S2. Add the pomelo peel powder to anhydrous ethanol in a 40 °C water bath at a solid-liquid ratio of 0.05 g / mL, ultrasonically treat it for 20 min, stir and react at 200 rpm for 12 h, perform suction filtration and separation, and then dry it to constant weight in a 60 °C vacuum environment to obtain modified powder;

[0056] S3. Add the modified powder to a 20 nm iron oxide nanosuspension (anhydrous ethanol), ultrasonically mix it for 12 h, and then perform magnetic separation using a magnet to obtain a magnetic carrier; the mass ratio of the modified powder to iron oxide is 1:0.2;

[0057] S4. Add the Lactobacillus plantarum powder and 20 nm magnetic iron oxide at a mixing ratio of 1:0.2 to physiological saline for incubation. Then, fix a magnet on the side of the container to cause the magnetic iron oxide to migrate towards the Lactobacillus plantarum like a magnet. After removing the supernatant, wash the precipitate and resuspend it in physiological saline to obtain a probiotic bacterial solution. Add the magnetic carrier to the probiotic bacterial solution, mix and load it in an alternating magnetic field of 0.3 mT - 0.4 mT (both the magnetic magnitude and direction change periodically) for 8 h, and then perform magnetic separation to obtain a probiotic carrier;

[0058] S5. Adsorb the probiotic carrier in a 2% sodium alginate solution and then separate it to obtain a gel precursor;

[0059] S6. Add the gel precursor to a 2% calcium chloride solution, ultrasonically disperse it for 20 min, stir and mix it for 30 min to form a gel, and then obtain a highly active synbiotic nutrient.

[0060] Example 2

[0061] Example 2 provides a method for preparing a highly active synbiotic nutrient, which is different from Example 1 in that in step S4, the magnetic carrier is directly added to the Lactobacillus plantarum bacterial solution and mixed and loaded in an alternating magnetic field of 0.3 mT - 0.4 mT (both the magnetic magnitude and direction change periodically).

[0062] Comparative Example 1

[0063] Comparative Example 1 provides a method for preparing a highly active synbiotic nutrient. The difference from Example 2 is that step S3 is not carried out, and the modified powder is directly added to the Lactobacillus plantarum bacterial liquid for mixing and loading in step S4.

[0064] Performance detection

[0065] The content of Lactobacillus plantarum loaded in the highly active synbiotic nutrients prepared in Example 1, Example 2 and Comparative Example 1 was measured. After the same gradient dilution was carried out respectively and then cultured in a conventional culture environment using MRS medium, the loading amount of Lactobacillus plantarum was detected by the standard plate counting method. The results are as follows Figure 2 shown. From Figure 2 it can be seen that by combining Example 1 and Example 2, after the Lactobacillus plantarum and the magnetic carrier are jointly given in Example 1, certain magnetic properties can be given to the Lactobacillus plantarum, so that the loading amount of probiotics can be increased when loading in an alternating magnetic field. However, in Comparative Example 1, when the modified powder is not magnetically treated, it is difficult to be uniformly compounded with the Lactobacillus plantarum in an alternating magnetic field environment, resulting in a decrease in the loading amount.

[0066] The highly active synbiotic nutrients prepared in Example 1, Example 2 and Comparative Example 1 were subjected to simulated gastrointestinal fluid release. The highly active synbiotic nutrients were respectively added to 10 mL of simulated gastric fluid (dissolving 9 g of sodium chloride in 1000 mL of distilled water, adding hydrochloric acid to adjust the pH to 2.0, and then adding 3.2 g of pepsin), stirred and digested at 37 °C at 140 rpm for 2 h, and then transferred to simulated intestinal fluid (using 6.8 g / L of KH2PO4 and 45 g / L of bile salts, adding 10 g / L of trypsin and adjusting the pH to 7.0), stirred at 37 °C at 140 rpm, sampled every 1 h, and the released Lactobacillus plantarum was measured using MRS medium and the standard plate counting method. The results are as follows Figure 3 shown. From Figure 3 it can be seen that the highly active synbiotic nutrient prepared in Example 1 has a higher release amount and a more stable release curve, because the structure of the nutrient prepared in Example 1 is more stable and has a higher probiotic loading amount, so that it can improve the protection and release ability of probiotics during gastrointestinal digestion.

[0067] The in vitro fermentation of the highly active synbiotic nutriments prepared in Example 1, Example 2 and Comparative Example 1 includes: pre-digesting the highly active synbiotic nutriments in simulated gastric juice for 2 h, and then placing them in simulated intestinal juice for 4 h to collect the digestive juice; collecting fresh feces (from healthy donors aged 20 - 24 years old who have not received any digestive or antibiotic treatment for at least three months) and adding PBS medium (containing 0.5 g / L of L-cysteine, 1 mg / L of resazurin, pH 7.4, autoclaved at 121 °C) with a mass three times that of the feces, vortexing thoroughly to mix, and filtering through four layers of gauze into a sterilized beaker to obtain a fecal suspension for standby; sucking 1 mL of digestive juice (corresponding to Example 1, Example 2, and Comparative Example 1 respectively) into a 20 mL serum bottle, adding 0.25 mL of 25% fecal suspension and 4.8 mL of BCM medium solution (each digestive juice sample is parallelly experimented three times); using fructooligosaccharide (FOS) as a positive control and using the simulated intestinal juice of the untreated nutriment as a blank control (Blank); after sealing the serum tubes, incubating them in a shaking incubator at 37 °C for 0 h, 3 h, 6 h, 12 h, 24 h, and 48 h, respectively taking 1 mL of the sample and centrifuging at 13,000 rpm for 5 min to separate the supernatant, and measuring the pH change of the supernatant as Figure 4 shown.

[0068] Taking 0.4 mL of the supernatant and adding it to a centrifuge tube, adding ultrapure water and 1 mol / L hydrochloric acid to acidify to pH 2 - 3, then vortexing and intermittently oscillating for 10 min, adding 0.01 mol / L of 2-ethylbutyric acid internal standard solution (final concentration of 1 mmol / L), vortexing and oscillating for 15 s, and then centrifuging at 13,000 rpm for 5 min to separate the upper layer supernatant; using an autosampler to inject 1 μL of the upper layer supernatant of the aliquot sample into a 7890B GC equipped with a capillary column (30 m x 250 μm x 0.25 μm, Agilent 19091F-433, HP-FFAP) (injection port temperature 220 °C; detector temperature 250 °C; FID flame ionization detector; temperature programming: initial column oven temperature 60 °C, lasting for 5 min, heating at 10 °C / min to 160 °C, holding for 2 min, heating at 20 °C / min to 220 °C, holding for 5 min; carrier gas is nitrogen, flow rate is 30 mL / min, split ratio is 10:1, fuel gas is hydrogen, flow rate is 35 mL / min, air flow rate is 400 mL / min) for analysis, calculating the identification and flame ionization detector response factors of acetic acid, propionic acid, and butyric acid relative to the internal standard (2-ethylbutyric acid) by injecting a short-chain fatty acid standard mixture, and measuring the peak areas of acetic acid, propionic acid, and butyric acid relative to 2-ethylbutyric acid to quantify the total short-chain fatty acid content in the sample, and the results are as Figure 5 shown.

[0069] Although the embodiments of the present invention have been described in detail above, it will be apparent to those skilled in the art that various modifications and variations can be made to these embodiments. However, it should be understood that such modifications and variations are all within the scope and spirit of the present invention as described in the claims. Moreover, the present invention as described herein may have other embodiments and can be implemented or realized in various ways.

Claims

1. A preparation method of a highly active synbiotic nutritional product, characterized in that, Comprising: Freeze-drying and pulverizing pomelo peel albedo to obtain pomelo peel powder; stirring and dispersing the pomelo peel powder in ethanol and then separating and drying to obtain modified powder; Mixing the modified powder with magnetic iron oxide nanosuspension and then separating to obtain magnetic carriers; mixing the magnetic carriers with probiotic bacteria solution in an alternating magnetic field for loading and then separating to obtain probiotic carriers; mixing and adsorbing the probiotic carriers in sodium alginate solution and then separating to obtain a gel precursor; ultrasonically dispersing the gel precursor in calcium ion solution and gel-setting to obtain a highly active synbiotic nutritional product.

2. The preparation method according to claim 1, characterized in that, The source of the pomelo peel albedo includes one of Shatian pomelo, Wendan pomelo, red-fleshed pomelo, juhong pomelo, Jinxiang pomelo, Pingshan pomelo, Jinlan pomelo, Anjiangxiang pomelo, Liangping pomelo, and late white pomelo; and / or, the probiotics in the probiotic bacteria solution include one of Lactobacillus plantarum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium bifidum, Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus paracasei, Lactococcus lactis, and Streptococcus thermophilus.

3. The preparation method according to claim 1, characterized in that, After freeze-drying and pulverizing the pomelo peel albedo, passing it through a 40-mesh to 100-mesh sieve to obtain pomelo peel powder; and / or, freeze-drying the pomelo peel albedo at -15°C to -20°C; and / or, freeze-drying the pomelo peel powder for 1 h - 2 h; and / or, stirring and dispersing the pomelo peel powder in ethanol and then performing ultrasonic treatment; and / or, stirring and dispersing the pomelo peel powder in ethanol at 30°C - 50°C.

4. The preparation method according to claim 1, characterized in that, The particle size of the magnetic iron oxide in the magnetic iron oxide nanosuspension is 10 nm - 50 nm; and / or, after mixing the modified powder with the magnetic iron oxide nanosuspension, performing ultrasonic treatment; and / or, the mass ratio of the magnetic iron oxide in the modified powder to the magnetic iron oxide nanosuspension is 1:(0.1 - 0.2); and / or, mixing the modified powder with the magnetic iron oxide nanosuspension for 10 h - 16 h.

5. The preparation method according to claim 1, characterized in that, Mixing the magnetic carriers with the probiotic bacteria solution in an alternating magnetic field of 0.3 mT - 0.4 mT; and / or, mixing and loading in the alternating magnetic field for 6 h - 10 h; and / or, performing magnetic separation after mixing and loading to obtain probiotic carriers; and / or, adsorbing the probiotic carriers in a sodium alginate solution with a concentration of 1% - 3%; and / or, drying the separated product under vacuum at 60°C - 80°C for 2 h - 5 h to obtain magnetic carriers.

6. The preparation method according to claim 1, wherein Stirring and dispersing the pomelo peel powder in ethanol for 8 h - 15 h; and / or, drying the separated product under vacuum at 50°C - 70°C to obtain modified powder; and / or, the calcium ion solution includes calcium chloride solution; and / or, ultrasonically dispersing in the calcium ion solution for 15 min - 30 min.

7. The preparation method according to claim 1, wherein When mixing the magnetic carriers with the probiotic bacteria solution in an alternating magnetic field, first mix and incubate the probiotic bacteria powder with the magnetic iron oxide nanosuspension in normal saline, then perform magnetic separation, remove the supernatant, and resuspend with normal saline to obtain the probiotic bacteria solution.

8. A highly active synbiotic nutritional product prepared by the preparation method according to any one of claims 1 to 7.

9. The highly active synbiotic nutritional product according to claim 8, wherein The loading amount of the probiotic in the nutritional product is 1×10 9 CFUs - 2×10 9 CFUs; and / or, the release rate of the nutritional product is 1×10 8 CFUs / h - 2×10 8 CFUs / h; and / or, the release time of the nutritional product for the probiotic is 6 h - 10 h.

10. An application of a highly active synbiotic nutritional product prepared by the preparation method according to any one of claims 1 to 7 or a highly active synbiotic nutritional product according to any one of claims 8 to 9.

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