A composite microbial enzyme synergistic fertilizer composition and a preparation method thereof

Abstract

The application relates to the technical field of biological fertilizer, in particular to a compound microbial enzyme synergistic fertilizer composition and a preparation method thereof. The preparation steps of the compound microbial enzyme synergistic fertilizer composition comprise the following steps: step 1, mixing poultry manure and mushroom residues, adding corn straw fragments to adjust the carbon-nitrogen ratio, and obtaining fermentation raw materials; step 2, adding laccase to the fermentation raw materials and inoculating a compound microbial agent, and performing aerobic fermentation to obtain primary compost; step 3, adding a compound enzyme agent to the primary compost and inoculating clostridium butyricum, and performing anaerobic fermentation to obtain final compost; and step 4, mixing the final compost and an adsorption carrier, adding a compound stabilizing liquid, uniformly stirring, low-temperature drying, and granulating to obtain the compound microbial enzyme synergistic fertilizer composition. The prepared fertilizer composition can significantly improve key growth indexes such as the plant height, stem diameter and disc diameter of sunflowers, effectively reduces the empty shell rate, greatly improves the single disc weight and the yield per mu, and significantly enhances the fertilizer utilization rate.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological fertilizer, more particularly, it relates to a compound microbial enzyme synergistic fertilizer composition and a preparation method thereof. BACKGROUND

[0002] The global soil microbial diversity is facing a severe situation of rapid decline. Long-term and large-scale use of chemical fertilizers has led to a significant decrease in the abundance of beneficial bacteria (such as nitrogen-fixing bacteria and phosphorus-solubilizing bacteria) in the soil, the structure of the microbial community has been destroyed, and the activity of soil enzymes (such as urease and sucrose) has also been greatly reduced. Traditional microbial fertilizers can partially supplement functional bacteria, but single strains have a low colonization rate in the soil and are easily inactivated by factors such as soil environment and heavy metals. In existing enzyme fertilizers, the half-life of enzymes such as cellulase and protease in the soil is short, and the nutrients need to be further converted into absorbable nutrients by microorganisms, resulting in a lag in nutrient release.

[0003] However, existing microbial enzyme compound fertilizers generally have functional antagonism problems. Organic acids (such as lactic acid) produced by microbial metabolism can reduce the activity of enzymes, and free amino acids released during enzyme hydrolysis can inhibit the proliferation of certain functional bacteria. In addition, the action timing of microbial enzymes does not match, resulting in low synergistic efficiency, limited fertilizer efficiency improvement, and other problems. Based on the above status quo, the present application provides a compound microbial enzyme synergistic fertilizer composition and a preparation method thereof, which is used to improve soil fertility, promote plant growth, reduce the amount of chemical fertilizers used, and achieve sustainable development of agriculture. SUMMARY

[0004] In order to solve the technical problems mentioned in the background art, the present application provides a compound microbial enzyme synergistic fertilizer composition and a preparation method thereof.

[0005] A preparation method of a compound microbial enzyme synergistic fertilizer composition, comprising the following steps:

[0006] Step 1, mixing poultry manure and mushroom residue to obtain a mixture, adding corn straw to the mixture to adjust the carbon-nitrogen ratio to 25-30:1, controlling the pH to 6.5-8.0, and the water content to 60-70% to obtain fermentation raw materials;

[0007] Step 2, adding laccase to the fermentation raw materials and inoculating a compound microbial inoculant, performing aerobic fermentation at 25-37℃, controlling the aeration amount per kilogram of fermentation raw materials to be 0.4-0.6 L / min, and continuously fermenting for 1-2 days to obtain primary compost;

[0008] Step 3, adding the primary compost to a sealed fermentation tank, adding a compound enzyme agent and inoculating Clostridium butyricum, performing anaerobic fermentation, controlling the fermentation temperature to be 25-32℃, and fermenting for 5-7 days to obtain final compost;

[0009] Step 4, mixing the final stage of compost and the adsorption carrier, adding the composite stabilizing solution, stirring uniformly, low-temperature drying, and granulating to obtain the composite microbial enzyme synergistic fertilizer composition.

[0010] Preferably, the mass ratio of poultry manure and mushroom residue in step 1 is 3-5:1-2.

[0011] Preferably, the amount of laccase added in step 2 is 0.5-1% of the fermentation raw material.

[0012] Preferably, the inoculation amount of the composite microbial agent in step 2 is 3-8% of the fermentation raw material.

[0013] Preferably, the composite microbial agent in step 2 is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 8-12:1-3:2-4.

[0014] Preferably, the amount of composite enzyme agent added in step 3 is 0.5-2% of the primary compost.

[0015] Preferably, the composite enzyme agent in step 3 is composed of xylanase, papain, and phytase with a mass ratio of 20-30:5-12:3-8.

[0016] Preferably, the inoculation amount of Clostridium butyricum in step 3 is 3-5% of the primary compost.

[0017] Preferably, the mass ratio of the final stage of compost, the adsorption carrier, and the composite stabilizing solution in step 4 is 10-12:15-35:20-35.

[0018] Preferably, the adsorption carrier in step 4 is one or more of zeolite powder, hulled rice chaff, diatomite, and vermiculite.

[0019] Preferably, the composite stabilizing solution in step 4 is composed of sodium lignosulfonate, pullulan, polyglutamic acid, and deionized water with a mass ratio of 1-3:1-2:2-5:80-120.

[0020] A composite microbial enzyme synergistic fertilizer composition is prepared by the above method.

[0021] In summary, the present application has the following beneficial effects:

[0022] In the preparation of fermentation raw material, poultry manure and mushroom residue are mixed in a certain proportion, and corn straw is used to adjust the carbon-nitrogen ratio. The manure provides nitrogen and organic matter, and the mushroom residue contains residual mycelium, which can be used as a microbial nutrient source. The addition of mushroom residue and corn straw can increase the porosity, improve the aeration, reduce the risk of pile adhesion, and facilitate the colonization of aerobic microorganisms.

[0023] The application adds laccase and compound microbial agent to fermentation raw materials, and performs aerobic fermentation treatment. First, laccase specifically decomposes lignin to release cellulose and hemicellulose, improves organic matter utilization rate, and shortens composting time. Laccase can also catalyze the oxidation of phenolic substances to generate quinone compounds, which have high reactivity and can inhibit the growth of pathogenic bacteria (such as Escherichia coli and Salmonella) and reduce ammonia gas volatilization. If added in the early stage of fermentation, it can not only shorten the composting time but also inhibit the generation of pathogenic bacteria and promote the colonization of compound microbial species. The use of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum for compound fermentation can not only quickly decompose proteins and starch to produce small molecule peptides and sugars, but also inhibit the growth of spoilage bacteria by the antibacterial substances produced by Bacillus subtilis. In addition, Trichoderma harzianum can parasitize pathogenic fungi to induce plant disease resistance, forming a "bacteria inhibition-disease prevention" synergistic system with Bacillus subtilis and Lactobacillus pentosus.

[0024] The application uses the directional decomposition of compound enzyme agent to degrade hemicellulose in straw into monosaccharides with xylanase, increase the soluble carbon source to provide carbon source for Clostridium butyricum, decompose uncomposted proteins into amino acids with papain to increase the available nature of fertilizer, activate the insoluble phosphate in soil with phytase to mineralize organic phosphorus into a form available to plants, and improve the phosphorus content. The compound enzyme agent precisely degrades the complex organic matter remaining in the primary compost. The butyric acid produced by Clostridium butyricum and the lactic acid produced by Lactobacillus pentosus fermentation play a synergistic effect, which can regulate soil microbial community, promote plant root development, improve the available nature of fertilizer, and enhance the stress resistance of crops. The application first uses laccase and compound microbial agent to convert solid organic matter into soluble small molecules (such as reducing sugar and amino acid) in an aerobic environment, providing high-quality carbon and nitrogen sources for anaerobic fermentation. Then, through the combination of anaerobic environment and anaerobic bacteria, the directional conversion of nutrients (such as the conversion of organic phosphorus into inorganic phosphorus) is realized, and bioactive metabolites (such as butyric acid) are accumulated. This phased synergistic strategy makes the fertilizer have the functions of supplying available nutrients, maintaining long-acting fertility, and improving soil health. The ultimate compost and adsorption carrier are mixed to increase porosity, improve water retention and air permeability of the fertilizer, avoid hardening, prolong the fertilizer effect, and the compound stabilizing liquid is compounded by lignin sulfonate sodium, pullulan and polyglutamic acid. The three form a stable network structure to realize long-acting slow release of nutrients and microbial protection. DETAILED DESCRIPTION

[0025] The application will be further described in detail below in combination with examples.

[0026] The Bacillus subtilis (product number: B98052), Trichoderma harzianum (product number: B81740), Lactobacillus pentosus (product number: B81252) and Clostridium butyricum (product number: BMZ109220) used in the examples and comparative examples of the present application are all purchased from Ningbo Mingzhou Biotechnology Co., Ltd.; the laccase (enzyme activity: 10,000) is purchased from Jiangsu Enming Biological Engineering Technology Co., Ltd.; the xylanase (enzyme activity: 100,000) is purchased from Hubei Haijia Biological Technology Co., Ltd.; the papain (enzyme activity: 100,000) is purchased from Shandong Fangchang Biological Technology Co., Ltd.; the phytase (enzyme activity: 10,000) is purchased from Hebei Jiuyu Biological Technology Co., Ltd.; the sodium lignosulfonate is purchased from Shandong Jinrong Chemical Technology Co., Ltd.; the pullulan is purchased from Nanjing Baiju Technology Co., Ltd.; the polyglutamic acid (article number: GF110) is purchased from Jinan Guofeng Chemical Co., Ltd.

[0027] Examples 1-3 and Comparative Examples 1-7 provide a composite microbial enzyme synergistic fertilizer composition and a preparation method thereof.

[0028] Example 1

[0029] A preparation method of a composite microbial enzyme synergistic fertilizer composition comprises the following preparation steps:

[0030] Step 1, mixing cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture, adding corn straw fragments into the mixture to adjust the carbon-nitrogen ratio to 25:1, controlling the pH to be 6.5, and controlling the water content to be 60% to obtain fermentation raw materials;

[0031] Step 2, adding laccase and inoculating a composite microbial inoculum into the fermentation raw materials, performing aerobic fermentation at 25°C, controlling the aeration amount to be 0.4 L / min per kilogram of fermentation raw materials, and continuously fermenting for 1 day to obtain primary matured materials, wherein the addition amount of laccase is 0.5% of the fermentation raw materials, the inoculation amount of the composite microbial inoculum is 3% of the fermentation raw materials, and the composite microbial inoculum is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable bacterial number ratio of 8:1:2;

[0032] Step 3, adding the primary matured materials into a sealed fermentation tank, adding a composite enzyme agent and inoculating Clostridium butyricum, performing anaerobic fermentation, controlling the fermentation temperature to be 25°C, and fermenting for 5 days to obtain final matured materials, wherein the addition amount of the composite enzyme agent is 0.5% of the primary matured materials, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary matured materials;

[0033] Step 4, the final stage of compost and rice chaff are mixed, and the composite stabilizing solution is added, stirred at a speed of 100 rpm for 20 min, and then dried at a low temperature of 40℃ for 12 h after uniform stirring, and granulated, to obtain the composite bacteria enzyme synergistic fertilizer composition, wherein the mass ratio of the final stage of compost, rice chaff and the composite stabilizing solution is 10:15:20, and the composite stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0034] Example 2

[0035] A preparation method of a composite bacteria enzyme synergistic fertilizer composition comprises the following preparation steps:

[0036] Step 1, cow dung and mushroom residues are mixed according to a mass ratio of 4:1.5 to obtain a mixture, corn straw is added to the mixture to adjust the carbon-nitrogen ratio to 28:1, the pH is controlled to be 7.2, and the water content is 650%, to obtain fermentation raw materials;

[0037] Step 2, laccase is added to the fermentation raw materials, and a composite inoculant is inoculated, aerobic fermentation is carried out at 32℃, the aeration amount is controlled to be 0.5 L / min per kilogram of fermentation raw materials, and the fermentation is continuously carried out for 1.5 days, to obtain primary compost, wherein the addition amount of laccase is 0.8% of the fermentation raw materials, and the inoculation amount of the composite inoculant is 5% of the fermentation raw materials; the composite inoculant is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable bacterial number ratio of 10:2:3;

[0038] Step 3, the primary compost is added to a sealed fermentation tank, a composite enzyme agent is added, and Clostridium butyricum is inoculated, anaerobic fermentation is carried out, the fermentation temperature is controlled to be 28℃, and the fermentation is carried out for 6 days, to obtain final compost, wherein the addition amount of the composite enzyme agent is 1% of the primary compost, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 25:8:5, and the inoculation amount of Clostridium butyricum is 4% of the primary compost;

[0039] Step 4, the final compost and rice chaff are mixed, and the composite stabilizing solution is added, stirred at a speed of 150 rpm for 25 min, and then dried at a low temperature of 45℃ for 18 h after uniform stirring, and granulated, to obtain the composite bacteria enzyme synergistic fertilizer composition, wherein the mass ratio of the final compost, rice chaff and the composite stabilizing solution is 11:25:28, and the composite stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 2:1.5:4:100.

[0040] Example 3

[0041] A preparation method of a composite bacteria enzyme synergistic fertilizer composition comprises the following preparation steps:

[0042] Step 1, mixing cow dung and mushroom residue according to the mass ratio of 5:2 to obtain a mixture, adding corn straw to the mixture to adjust the carbon-nitrogen ratio to 30:1, controlling the pH to be 8.0, and the water content to be 70% to obtain fermentation raw materials;

[0043] Step 2, adding laccase and inoculating a composite microbial inoculum to the fermentation raw materials, performing aerobic fermentation at 37°C, controlling the aeration amount to be 0.6 L / min per kilogram of fermentation raw materials, and continuously fermenting for 2 days to obtain primary humus, wherein the addition amount of laccase is 1% of the fermentation raw materials, and the inoculation amount of the composite microbial inoculum is 8% of the fermentation raw materials; the composite microbial inoculum is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable bacterial ratio of 12:3:4;

[0044] Step 3, adding a composite enzyme agent and inoculating Clostridium butyricum to the primary humus in a sealed fermentation tank to perform anaerobic fermentation, controlling the fermentation temperature to be 32°C, and fermenting for 7 days to obtain final humus, wherein the addition amount of the composite enzyme agent is 2% of the primary humus, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 30:12:8, and the inoculation amount of Clostridium butyricum is 5% of the primary humus;

[0045] Step 4, mixing the final humus and rice chaff, adding a composite stabilizing liquid, stirring at a speed of 200 rpm for 30 min, and then low-temperature drying at 50°C for 24 h to obtain a composite microbial enzyme synergistic fertilizer composition, wherein the mass ratio of the final humus, the rice chaff and the composite stabilizing liquid is 12:35:35, and the composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 3:2:5:120.

[0046] Comparative Example 1

[0047] A preparation method of a composite microbial enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0048] Step 1, mixing cow dung and mushroom residue according to the mass ratio of 3:1 to obtain a mixture, adding corn straw to the mixture to adjust the carbon-nitrogen ratio to 25:1, controlling the pH to be 6.5, and the water content to be 60% to obtain fermentation raw materials;

[0049] Step 2, inoculating a composite microbial inoculum to the fermentation raw materials, performing aerobic fermentation at 25°C, controlling the aeration amount to be 0.4 L / min per kilogram of fermentation raw materials, and continuously fermenting for 1 day to obtain primary humus, wherein the inoculation amount of the composite microbial inoculum is 3% of the fermentation raw materials; the composite microbial inoculum is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable bacterial ratio of 8:1:2;

[0050] Step 3, add the primary mature material into a sealed fermentation tank, add a composite enzyme agent and inoculate Clostridium butyricum to perform anaerobic fermentation, control the fermentation temperature to be 25℃, and ferment for 5 days to obtain the final mature material, wherein the adding amount of the composite enzyme agent is 0.5% of the primary mature material, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary mature material;

[0051] Step 4, mix the final mature material and rice chaff, add a composite stabilizing liquid, stir at a speed of 100 rpm for 20 min, and then dry at a low temperature of 40℃ for 12 h after uniform stirring to obtain the composite bacteria enzyme synergistic fertilizer composition, wherein the mass ratio of the final mature material, the rice chaff and the composite stabilizing liquid is 10:15:20, and the composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0052] Comparative Example 2

[0053] A preparation method of a composite bacteria enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0054] Step 1, mix cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture, add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to be 6.5, and control the water content to be 60% to obtain fermentation raw materials;

[0055] Step 2, add laccase to the fermentation raw materials and inoculate a composite microbial agent, perform aerobic fermentation at 25℃, control the aeration amount per kilogram of fermentation raw materials to be 0.4 L / min, and continuously ferment for 1 day to obtain primary mature material, wherein the adding amount of laccase is 0.5% of the fermentation raw materials, the inoculation amount of the composite microbial agent is 3% of the fermentation raw materials, and the composite microbial agent is composed of Bacillus subtilis and Trichoderma harzianum with an effective viable bacterial number ratio of 8:2;

[0056] Step 3, add the primary mature material into a sealed fermentation tank, add a composite enzyme agent and inoculate Clostridium butyricum to perform anaerobic fermentation, control the fermentation temperature to be 25℃, and ferment for 5 days to obtain the final mature material, wherein the adding amount of the composite enzyme agent is 0.5% of the primary mature material, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary mature material;

[0057] Step 4, the final stage of the mixture of rotten material and rice chaff, add composite stabilizing liquid, stirring speed is 100 rpm, stirring 20 min, after stirring evenly, 40 ℃ low temperature drying 12 h, granulation, namely the composite bacteria enzyme synergistic fertilizer composition, wherein, the final stage of the mixture of rotten material, rice chaff and composite stabilizing liquid is 10:15:20, the composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0058] Comparative example 3

[0059] A preparation method of a composite bacteria enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0060] Step 1, the mixture of cattle manure and mushroom residue is mixed according to the mass ratio of 3:1, the mixture is added to corn straw to adjust the carbon-nitrogen ratio to 25:1, the pH is controlled to 6.5, and the water content is 60%, thereby obtaining fermentation raw materials;

[0061] Step 2, laccase is added to the fermentation raw materials and inoculated with a composite bacterial agent, aerobic fermentation is carried out at 25℃, the aeration amount is controlled to be 0.4 L / min per kilogram of fermentation raw materials, and the fermentation is continuously carried out for 1 day, thereby obtaining primary rotten material, wherein the addition amount of laccase is 0.5% of the fermentation raw materials, and the inoculation amount of the composite bacterial agent is 3% of the fermentation raw materials; the composite bacterial agent is composed of bacillus subtilis and lactobacillus pentosus with an effective viable bacterial number ratio of 8:1;

[0062] Step 3, the primary rotten material is added to a sealed fermentation tank, a composite enzyme agent is added and inoculated with butyric acid clostridium, anaerobic fermentation is carried out, the fermentation temperature is controlled to be 25℃, and the fermentation is carried out for 5 days, thereby obtaining final rotten material, wherein the addition amount of the composite enzyme agent is 0.5% of the primary rotten material, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of butyric acid clostridium is 3% of the primary rotten material;

[0063] Step 4, the final rotten material and rice chaff are mixed, a composite stabilizing liquid is added, stirring speed is 100 rpm, stirring 20 min, after stirring evenly, 40 ℃ low temperature drying 12 h, granulation, namely the composite bacteria enzyme synergistic fertilizer composition, wherein, the final stage of the mixture of rotten material, rice chaff and composite stabilizing liquid is 10:15:20, the composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0064] Comparative example 4

[0065] A preparation method of a composite bacteria enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0066] Step 1, mixing cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture, adding corn straw fragments into the mixture to adjust a carbon-nitrogen ratio to 25:1, controlling pH to be 6.5, and controlling water content to be 60% to obtain fermentation raw materials;

[0067] Step 2, adding laccase into the fermentation raw materials and inoculating a composite microbial inoculum, performing aerobic fermentation at 25℃, controlling aeration amount to be 0.4 L / min per kilogram of fermentation raw materials, and continuously fermenting for 1 day to obtain primary humus, wherein the adding amount of laccase is 0.5% of the fermentation raw materials, the inoculating amount of the composite microbial inoculum is 3% of the fermentation raw materials, and the composite microbial inoculum is composed of bacillus subtilis, lactobacillus pentosus and trichoderma harzianum with an effective viable bacterial ratio of 8:1:2;

[0068] Step 3, adding the primary humus into a sealed fermentation tank and adding a composite enzyme agent to perform anaerobic fermentation, controlling fermentation temperature to be 25℃, and fermenting for 5 days to obtain final humus, wherein the adding amount of the composite enzyme agent is 0.5% of the primary humus, and the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3;

[0069] Step 4, mixing the final humus and rice chaff, adding a composite stabilizing liquid, stirring at a rotating speed of 100 rpm for 20 min, low-temperature drying at 40℃ for 12 h after uniform stirring, and granulating to obtain the composite microbial enzyme synergistic fertilizer composition.

[0070] Comparative Example 5

[0071] A preparation method of a composite microbial enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0072] Step 1, mixing cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture, adding corn straw fragments into the mixture to adjust a carbon-nitrogen ratio to 25:1, controlling pH to be 6.5, and controlling water content to be 60% to obtain fermentation raw materials;

[0073] Step 2, adding laccase into the fermentation raw materials and inoculating a composite microbial inoculum, performing aerobic fermentation at 25℃, controlling aeration amount to be 0.4 L / min per kilogram of fermentation raw materials, and continuously fermenting for 1 day to obtain primary humus, wherein the adding amount of laccase is 0.5% of the fermentation raw materials, the inoculating amount of the composite microbial inoculum is 3% of the fermentation raw materials, and the composite microbial inoculum is composed of bacillus subtilis, lactobacillus pentosus and trichoderma harzianum with an effective viable bacterial ratio of 8:1:2;

[0074] Step 3, add the primary mature material into a sealed fermentation tank, add a composite enzyme agent and inoculate Clostridium butyricum, perform anaerobic fermentation, control the fermentation temperature to be 25℃, and ferment for 5 days to obtain the final mature material, wherein the adding amount of the composite enzyme agent is 0.5% of the primary mature material, the composite enzyme agent is composed of xylanase and phytase at a mass ratio of 20:3, and the inoculation amount of Clostridium butyricum is 3% of the primary mature material;

[0075] Step 4, mix the final mature material and rice chaff, add a composite stabilizing liquid, stir at a speed of 100 rpm for 20 min, and then dry at a low temperature of 40℃ for 12 h to obtain the composite microbial enzyme synergistic fertilizer composition, wherein the mass ratio of the final mature material, the rice chaff and the composite stabilizing liquid is 10:15:20, and the composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water at a mass ratio of 1:1:2:80.

[0076] Comparative Example 6

[0077] A preparation method of a composite microbial enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0078] Step 1, mix cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture, add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to be 6.5, and control the water content to be 60% to obtain fermentation raw materials;

[0079] Step 2, add laccase to the fermentation raw materials and inoculate a composite microbial agent, perform aerobic fermentation at 25℃, control the aeration amount per kilogram of fermentation raw materials to be 0.4 L / min, and continuously ferment for 1 day to obtain primary mature material, wherein the adding amount of laccase is 0.5% of the fermentation raw materials, the inoculation amount of the composite microbial agent is 3% of the fermentation raw materials, and the composite microbial agent is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum at an effective viable bacterial number ratio of 8:1:2;

[0080] Step 3, add the primary mature material into a sealed fermentation tank, add a composite enzyme agent and inoculate Clostridium butyricum, perform anaerobic fermentation, control the fermentation temperature to be 25℃, and ferment for 5 days to obtain the final mature material, wherein the adding amount of the composite enzyme agent is 0.5% of the primary mature material, the composite enzyme agent is composed of xylanase and phytase at a mass ratio of 20:3, and the inoculation amount of Clostridium butyricum is 3% of the primary mature material;

[0081] Step 4, the final stage of compost and rice chaff are mixed, and the composite stabilizing solution is added, stirred at 100 rpm for 20 min, and then dried at 40℃ for 12 h after uniform stirring, and granulated to obtain the composite bacteria enzyme synergistic fertilizer composition, wherein the mass ratio of the final stage of compost, rice chaff and the composite stabilizing solution is 10:15:20, and the composite stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0082] Comparative Example 7

[0083] A preparation method of a composite bacteria enzyme synergistic fertilizer composition, comprising the following preparation steps:

[0084] Step 1, cow dung and mushroom residues are mixed according to a mass ratio of 3:1 to obtain a mixture, corn straw is added to the mixture to adjust the carbon-nitrogen ratio to 25:1, the pH is controlled to be 6.5, and the water content is 60% to obtain fermentation raw materials;

[0085] Step 2, the composite bacteria agent is inoculated into the fermentation raw materials, aerobic fermentation is carried out at 25℃, the aeration amount is controlled to be 0.4 L / min per kilogram of fermentation raw materials, and the fermentation is continued for 1 day to obtain primary compost, wherein the inoculation amount of the composite bacteria agent is 3% of the fermentation raw materials; the composite bacteria agent is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable bacterial number ratio of 8:1:2;

[0086] Step 3, the primary compost is added into a sealed fermentation tank, the composite enzyme agent is added and Clostridium butyricum is inoculated, anaerobic fermentation is carried out, the fermentation temperature is controlled to be 25℃, and the fermentation is continued for 5 days to obtain final compost, wherein the addition amount of the composite enzyme agent is 1% of the primary compost, the composite enzyme agent is composed of laccase, xylanase, papain and phytase with a mass ratio of 28:20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary compost;

[0087] Step 4, the final compost and rice chaff are mixed, and the composite stabilizing solution is added, stirred at 100 rpm for 20 min, and then dried at 40℃ for 12 h after uniform stirring, and granulated to obtain the composite bacteria enzyme synergistic fertilizer composition, wherein the mass ratio of the final stage of compost, rice chaff and the composite stabilizing solution is 10:15:20, and the composite stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0088] Performance test

[0089] The comprehensive performance of the composite bacteria enzyme synergistic fertilizer composition (bacteria enzyme composite fertilizer) prepared in Examples 1-3 and Comparative Examples 1-7 of the present application is detected respectively, and the details are as follows:

[0090] 1. Basic index detection of the fertilizer: The test results are shown in Table 1, which is determined according to the industry standard NY / T 525-2021 "Organic Fertilizer" of the Ministry of Agriculture and Rural Affairs;

[0091] Table 1 Comprehensive performance of the bacteria-enzyme compound fertilizer prepared in Examples 1-3 and Comparative Examples 1-7

[0092] Test performance Organic matter content (%) Total nutrient (%) Seed germination rate (%) pH Odor grade Example 1 56.76 9.84 96.4 5.83 Grade 1 Example 2 53.36 9.43 94.5 6.28 Grade 1 Example 3 51.54 8.94 95.1 6.54 Grade 1 Comparative Example 1 41.46 5.37 80.4 6.35 Grade 3 Comparative Example 2 48.35 6.32 88.5 7.46 Grade 3 Comparative Example 3 47.29 6.13 87.2 6.34 Grade 2 Comparative Example 4 45.89 5.98 85.4 7.58 Grade 3 Comparative Example 5 40.63 5.22 82.1 6.74 Grade 2 Comparative Example 6 38.68 4.63 73.7 6.53 Grade 2 Comparative Example 7 43.75 5.75 84.8 6.62 Grade 3

[0093] 2. Yield-increasing test

[0094] Test site: Yaqian Village, Laishan District, Yantai City, Shandong Province, the test site is set as 12 test points, each test point has an area of 10 mu, and is respectively recorded as Example Groups 1-3, Comparative Example Groups 1-7, Test Control Group and Blank Control Group;

[0095] Test variety: sunflower SH361;

[0096] Test time: April 15, 2023-September 25, 2023;

[0097] Test design: the bacteria-enzyme compound fertilizers prepared in Examples 1-3 are respectively applied as base fertilizer at 30 kg / mu in Example Groups 1-3; the bacteria-enzyme compound fertilizers prepared in Comparative Examples 1-7 are respectively applied as base fertilizer at 30 kg / mu in Comparative Example Groups 1-7, conventional base fertilizer (conventional base fertilizer: monoammonium phosphate 30 kg / mu) is applied in Test Control Group, and no base fertilizer is applied in Blank Control Group, topdressing of 15 kg / mu of monoammonium phosphate, 12 kg / mu of urea and 5 kg / mu of potassium chloride is applied in each test point, and each test point is managed to achieve "five unifications", i.e., unified sunflower variety, unified sowing time, unified fertilization amount, unified sowing density and unified field management;

[0098] Test method: the test site is deeply applied and covered with soil in sowing row on April 15, covered with mulch and applied with base fertilizer, watered on April 20, sunflower seeds SH361 are sowed by point on the side of the fertilization ditch on May 1, with large row spacing of 90 cm, small row spacing of 40 cm and plant spacing of 60 cm, 1700 plants per mu are left, topdressing is performed once on June 28, and 10 plants are randomly taken on September 25 for determination of single plant performance and yield per mu;

[0099] The test results are shown in Table 2.

[0100] Table 2 Traits and yield per mu of sunflower treated with different fertilizers

[0101] Test performance Plant height (cm) Stem thickness (cm) Disc diameter (cm) Husk rate (%) Single disc weight (kg) Yield per mu (kg) Example group 1 217.4 4.13 25.36 13.62 1.256 256.32 Example group 2 215.7 4.15 25.67 14.28 1.198 248.53 Example group 3 220.5 4.29 25.94 12.47 1.389 267.75 Comparative example group 1 203.5 3.47 22.25 18.35 1.039 228.17 Comparative example group 2 208.5 3.75 23.16 17.79 1.093 232.36 Comparative example group 3 210.3 3.88 23.65 16.82 1.177 238.10 Comparative example group 4 208.1 3.69 22.94 17.73 1.114 230.86 Comparative example group 5 204.8 3.52 22.71 18.13 1.057 229.34 Comparative example group 6 198.7 3.26 21.93 19.36 1.043 217.89 Comparative example group 7 207.4 3.58 22.68 17.67 1.103 229.24 Test control group 195.5 3.11 21.35 17.12 0.993 215.64 Blank control group 189.3 2.67 19.37 21.25 0.678 179.43

[0102] As can be seen from Table 1 and Table 2, the composite enzyme synergistic fertilizer composition prepared in the application is not only obviously superior to the industry standard of the Ministry of Agriculture and Rural Affairs for organic fertilizer, and in the application of sunflower planting, the enzyme composite fertilizer can significantly improve the key growth indexes such as the plant height, stem diameter and disc diameter of sunflower, effectively reduce the empty shell rate, greatly improve the single disc weight and yield per mu, significantly enhance the fertilizer utilization rate, and bring significant yield-increasing and efficiency-increasing results for sunflower planting.

[0103] The specific embodiments are only an explanation of the application, and are not a limitation of the application, and those skilled in the art can make modifications to the embodiments without creative contribution according to the needs after reading the specification, but as long as the modifications are within the scope of the claims of the application, they are protected by the Patent Law.

Claims

1. A method for preparing a complex microbial enzyme synergistic fertilizer composition, characterized in that, The preparation method comprises the following steps: Step 1, mixing poultry manure and mushroom residue to obtain a mixture, adding corn straw to the mixture to adjust the carbon-nitrogen ratio to 25-30:1, controlling the pH to be 6.5-8.0, and controlling the water content to be 60-70% to obtain fermentation raw materials; Step 2, adding laccase and inoculating a composite microbial inoculum to the fermentation raw materials, performing aerobic fermentation at 25-37℃, controlling the aeration amount to be 0.4-0.6 L / min per kilogram of fermentation raw materials, and continuously fermenting for 1-2 days to obtain primary compost; Step 3, adding the primary compost to a sealed fermentation tank, adding a composite enzyme agent and inoculating Clostridium butyricum, performing anaerobic fermentation, controlling the fermentation temperature to be 25-32℃, and fermenting for 5-7 days to obtain final compost; Step 4, mixing the final compost and an adsorption carrier, adding a composite stabilizing liquid, uniformly stirring, low-temperature drying, and granulating to obtain the composite microbial enzyme synergistic fertilizer composition; The inoculation amount of the composite microbial inoculum in step 2 is 3-8% of the fermentation raw materials; the composite microbial inoculum is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable bacterial number ratio of 8-12:1-3:2-4; The addition amount of the composite enzyme agent in step 3 is 0.5-2% of the primary compost; the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20-30:5-12:3-8; The composite stabilizing liquid in step 4 is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1-3:1-2:2-5:80-120.

2. The preparation method of the compound bacterial enzyme synergistic fertilizer composition according to claim 1, characterized in that, The mass ratio of poultry manure to mushroom residue in step 1 is 3-5:1-2.

3. The preparation method of the compound bacterial enzyme synergistic fertilizer composition according to claim 1, characterized in that, The addition amount of laccase in step 2 is 0.5-1% of the fermentation raw materials.

4. The method of claim 1, wherein the complex microbial enzyme synergistic fertilizer composition is prepared by the steps of: (a) mixing the microbial enzyme with the inorganic fertilizer; (b) drying the mixture; (c) mixing the dried mixture with the organic fertilizer; and (d) drying the mixture. The inoculation amount of Clostridium butyricum in step 3 is 3-5% of the primary compost.

5. The method of claim 1, wherein the complex microbial enzyme synergistic fertilizer composition is prepared by the steps of: a) mixing the microbial enzyme with the inorganic fertilizer; b) drying the mixture; c) mixing the dried mixture with the organic fertilizer; and d) drying the mixture. The mass ratio of the final compost, the adsorption carrier and the composite stabilizing liquid in step 4 is 10-12:15-35:20-35.

6. The method of claim 1, wherein the complex microbial enzyme synergistic fertilizer composition is prepared by the steps of: a) mixing the microbial enzyme with the inorganic fertilizer; b) drying the mixture; c) mixing the dried mixture with the organic fertilizer; and d) drying the mixture. The adsorption carrier in step 4 is one or more of zeolite powder, hulled rice chaff, diatomite and vermiculite.

7. A composite microbial enzyme synergistic fertilizer composition prepared by the preparation method of any one of claims 1-6.