Needle-free electrostatic spinning preparation method and application of probiotic mask

Abstract

The invention belongs to the technical field of skin care, and particularly relates to a needleless electrostatic spinning preparation method and application of a probiotic mask, the probiotic mask prepared by the needleless electrostatic spinning preparation method of the probiotic mask provided by the invention realizes instant dissolution in water and release of probiotics, can better meet modern skin care requirements, and has good application prospects. The skin care effect of the probiotics is exerted; meanwhile, the skin care effect of the probiotic mask is further improved by improving the variety of water-soluble polymers, the variety of probiotics, the volume ratio of a water-soluble polymer solution (basic spinning solution) to a probiotic solution and the like; therefore, the technical problem that in the prior art, it is difficult for probiotics to effectively exert the skin care effect is solved.

Description

Technical Field

[0001] This application belongs to the technical field of skin care, and particularly relates to a needleless electrospinning preparation method and application of a probiotic mask. Background Art

[0002] In recent years, with the rise of the concept of "microecological skin care", more and more people have begun to pay attention to the microecological balance of the skin; the so-called microecological environment refers to the living environment of the microbial colonies on the skin surface. In fact, not only inside the human body, but also on the epidermal skin of the human body, there are microbial colonies. The microbial environment on the skin surface is composed of harmful bacteria and beneficial bacteria. They maintain the stability of the microbial community environment through mutual restraint, and the skin condition and defense ability can be maintained at a normal level. The normal microbial community environment can inhibit the occurrence of skin inflammation and defend against the damage caused by potential pathogenic bacteria to the skin. Therefore, it has an important impact on the skin.

[0003] In recent years, some literature reports that some probiotics have skin care effects. For example, Patent CN115404189A discloses that Lactobacillus coryniformis SEUNEU-116 isolated from fermented perilla leaves of the Korean ethnic group has the functions of maintaining and repairing the skin barrier, anti-inflammatory, inhibiting skin pathogens, and treating acne. There is also Patent CN115501143A that discloses an anti-allergic and soothing composition containing snow lotus cultures, in which probiotics are used to stimulate other various extracts to produce more active ingredients and make them smaller molecules, so that the active ingredients can better penetrate into the dermis of the skin, promote the growth of keratinocytes, accelerate the renewal of the stratum corneum, promote the proliferation of skin beneficial bacteria, improve the living environment of the defensive skin flora, enhance the skin moisturizing ability and barrier ability, and make the skin feel smoother and finer.

[0004] At present, the main way to preserve probiotics is to make them into freeze-dried powders through freeze-drying technology, and dissolve the probiotic freeze-dried powders in water when in use; however, when using probiotics for skin care, this way of using probiotic freeze-dried powders is too complicated and does not meet the needs of modern skin care, and the skin care efficacy of probiotics is difficult to fully exert. Summary of the Invention

[0005] In view of this, this application provides a needleless electrospinning preparation method and application of a probiotic mask to solve the technical problem that probiotics are difficult to effectively exert their skin care efficacy in the prior art.

[0006] The first aspect of this application provides a needleless electrospinning preparation method of a probiotic mask, including the following steps:

[0007] Steps of preparing a basic spinning solution, dissolving a water-soluble polymer in sterile ionized water to obtain a basic spinning solution;

[0008] Steps for preparing a probiotic solution: Mix the freeze-dried probiotic powder and sterile ionic water evenly to obtain a probiotic solution;

[0009] Steps for preparing a probiotic spinning solution: Mix the probiotic solution with a basic spinning solution to obtain a probiotic spinning solution;

[0010] Steps for preparing a probiotic facial mask: Add the probiotic spinning solution into the injection pump of a needleless electrospinning device, and start the needleless electrospinning device to perform electrospinning to obtain a probiotic facial mask.

[0011] Preferably, in the steps for preparing the basic spinning solution, the water-soluble polymer is selected from at least one of polyethylene oxide, polyvinyl alcohol, and polyvinylpyrrolidone.

[0012] Preferably, the water-soluble polymer is selected from polyvinyl alcohol with an alcoholysis degree of 87%-89%.

[0013] Preferably, the water-soluble polymer is selected from polyethylene oxide with a molecular weight of 300,000 to 500,000.

[0014] Preferably, in the steps for preparing the probiotic solution, the probiotics in the freeze-dried probiotic powder are selected from at least one of freeze-dried powder of Lactobacillus delbrueckii, freeze-dried powder of Lactobacillus rhamnosus, and freeze-dried powder of Lactobacillus paracasei.

[0015] Preferably, in the steps for preparing the probiotic spinning solution, calculated by mass, the probiotic spinning solution includes 1 to 5 parts by mass of probiotics, 4 to 10 parts by mass of water-soluble polymer, and 85 to 95 parts by mass of sterile ionic water.

[0016] Preferably, in the steps for preparing the probiotic spinning solution, calculated by volume, the volume ratio of the probiotic solution to the basic spinning solution in the probiotic spinning solution is 1 to 9:10.

[0017] Preferably, the probiotic solution is a probiotic solution of 100 to 1000 CFU / ml;

[0018] The basic spinning solution is a basic spinning solution of 5 to 20 w / v.

[0019] Preferably, in the steps for preparing the probiotic facial mask, the liquid supply speed of the injection pump in the needleless electrospinning device is 100 to 1000 ml / h.

[0020] Preferably, in the steps for preparing the probiotic facial mask, the positive voltage / current of the electrospinning is 50.34 kV / 2.24 mA, and the negative voltage / negative current is .

[0021] Preferably, in the steps of preparing the probiotic mask, the coating speed of the electrospinning is 60-100 mm / s, the collection distance is 15-25 cm, the winding speed is 0.1-0.5 m / min, and the conveyor belt speed is 0.1-0.5 m / min.

[0022] Preferably, in the steps of preparing the probiotic mask, the atmosphere of the electrospinning is a nitrogen atmosphere, the spinning temperature is 10-20 °C, and the ambient humidity is 10%RH.

[0023] The second aspect of the present application provides an application of a probiotic mask prepared by the needleless electrospinning preparation method described in the first aspect in the field of skin care.

[0024] Compared with the prior art, the needleless electrospinning preparation method of a probiotic mask provided by the present application has at least the following beneficial effects:

[0025] 1. The needleless electrospinning preparation method of a probiotic mask provided by the present application uses the needleless electrospinning process to avoid the situation that the volume of the strains in the probiotic spinning solution is too large and easily blocks the needle during the preparation process, realizing the batch preparation of probiotic masks. At the same time, the probiotic mask has a high porosity and a large specific surface area. On the one hand, it can effectively load probiotics, and on the other hand, it can better contact with media such as water. Coupled with the fact that the material of the spinning fibers in the probiotic mask is a water-soluble polymer, the effect of quickly dissolving in water and releasing probiotics is better, which can better meet the modern skin care needs and exert the probiotic skin care effect.

[0026] 2. The needleless electrospinning preparation method of a probiotic mask provided by the present application improves the skin care effect of the probiotic mask by improving the water-soluble polymer and the types of probiotics.

[0027] 3. The needleless electrospinning preparation method of a probiotic mask provided by the present application improves the skin care effect of the probiotic mask by improving the volume ratio of the water-soluble polymer solution (basic spinning solution) to the probiotic solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0029] Figure 1 It is a schematic structural diagram of a needleless electrospinning device in the needleless electrospinning preparation method of a probiotic mask provided in Embodiments 1-8 of the present application;

[0030] Figure 2 The needleless electrospinning preparation method of the probiotic mask provided in Embodiment 4 of the present application, and the diameter characterization results of the nanofibers in the prepared probiotic mask;

[0031] Figure 3 The needleless electrospinning preparation method of the probiotic mask provided in Embodiment 5 of the present application, and the diameter characterization results of the nanofibers in the prepared probiotic mask;

[0032] Figure 4 The needleless electrospinning preparation method of the probiotic mask provided in Embodiment 6 of the present application, and the diameter characterization results of the nanofibers in the prepared probiotic mask;

[0033] Figure 5 The needleless electrospinning preparation method of the probiotic mask provided in Embodiment 7 of the present application, and the diameter characterization results of the nanofibers in the prepared probiotic mask;

[0034] Figure 6 The needleless electrospinning preparation method of the probiotic mask provided in Embodiment 8 of the present application, and the diameter characterization results of the nanofibers in the prepared probiotic mask;

[0035] Figure 7 The needleless electrospinning preparation method of the probiotic mask provided in Embodiments 4-8 of the present application, and the results of the average diameter and standard deviation of the nanofibers in the prepared probiotic mask;

[0036] Figure 8 The needleless electrospinning preparation method of the probiotic mask provided in Embodiments 4-8 of the present application, and the test results of the instant solubility effect of the prepared probiotic mask;

[0037] Figure 9 The needleless electrospinning preparation method of the probiotic mask provided in Embodiments 4-8 of the present application, and the test results of the skin care effect of the prepared probiotic mask. Detailed implementation manners

[0038] The present application provides a needleless electrospinning preparation method and application of a probiotic mask, which are used to solve the technical problem that probiotics are difficult to effectively exert skin care effects in the prior art.

[0039] Next, the technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative efforts shall fall within the protection scope of the present application.

[0040] In view of the fact that the current usage form of probiotic freeze-dried powder does not meet the requirements of modern skin care and has the defect of being difficult to effectively exert skin care effects; the present technical solution provides a needleless electrospinning preparation method for a probiotic mask; the preparation method includes: dissolving a water-soluble polymer in sterile ionized water to obtain a basic spinning solution; mixing the probiotic freeze-dried powder and sterile ionized water evenly to obtain a probiotic solution; mixing the probiotic solution with the basic spinning solution to obtain a probiotic spinning solution; adding the probiotic spinning solution into the injection pump of a needleless electrospinning device, and starting the needleless electrospinning device to perform electrospinning to obtain a probiotic mask.

[0041] The needleless electrospinning preparation method for the probiotic mask provided by the present application makes a probiotic spinning solution from probiotics and a water-soluble polymer and performs electrospinning to obtain a probiotic mask. The spinning fibers in the probiotic mask prepared by the electrospinning technology have characteristics such as high porosity and large specific surface area, providing sufficient space for probiotics, being beneficial to the adhesion of probiotics, enabling the effective attachment of probiotics. At the same time, the electrospinning process used in the present application is a needleless electrospinning process, avoiding the situation that when using a needle electrospinning process, the volume of the strains in the probiotic spinning solution is too large, prone to clogging the needle, and there will also be electric field interference between multiple groups of needles, resulting in a low spinning efficiency and the inability to mass-produce probiotic masks; in addition, in the probiotic mask prepared by the needleless electrospinning preparation method provided by the present application, the material of the spinning fibers is a water-soluble polymer, which enables the probiotic mask to quickly dissolve and release probiotics when encountering water. When performing skin care, the probiotic mask can be applied to the face, and then a spray bottle filled with deionized water is used to spray the mask, so that the probiotic mask quickly dissolves and releases probiotics to care for the skin. Making the probiotics into the form of a mask meets the requirements of modern skin care, and the ability to quickly dissolve in water can also effectively exert the skin care effects of probiotics; thus overcoming the defect that the traditional usage form of probiotic freeze-dried powder does not meet the requirements of modern skin care and is difficult to effectively exert skin care effects.

[0042] As a preferred technical solution, in the process of preparing the probiotic facial mask provided by the present application, the water-soluble polymer used can be selected from polyethylene oxide, polyvinyl alcohol, polyvinyl pyrrolidone etc., and probiotics can be selected from probiotics such as lactobacillus delbrueckii freeze-dried powder, lactobacillus rhamnosus freeze-dried powder, and lactobacillus paracasei; These water-soluble polymers have good biocompatibility, and are soluble in water and release probiotics for skin care; Further, water-soluble polymers are selected from polyvinyl alcohol with 87%-89% alcoholysis degree, and probiotics select lactobacillus paracasei, and when this probiotic facial mask is applied to the face, it is possible to achieve a good moisturizing and oil-removing skin care effect, and the moisturizing and oil-removing effect is improved along with the content of probiotics. Of course, probiotics can also select lactobacillus delbrueckii and lactobacillus rhamnosus as composite strains, and the two bacteria can cooperate with each other, act together on the skin surface, remove skin surface free radicals, optimize skin barrier, greatly improve the probiotic facial mask moisturizing and hydrating, anti-allergic and anti-aging abilities, and skin care effect is improved.

[0043] As a preferred technical solution, in the process of preparing the probiotic mask provided by the present application, it is necessary to control the amount of probiotic spinning solution and basic spinning solution. Taking the basic spinning solution and probiotic solution with concentrations of 10w / v and 500CFU / ml as an example, when the ratio of the two is different, it will affect the formation of nanofibers in the probiotic mask. When the ratio of the probiotic solution is 10~40% of the basic spinning solution, as the proportion of the probiotic solution increases, The viscosity of the spinning solution gradually increases, which makes the fiber less likely to be stretched during the forming process, resulting in a gradual increase in the fiber diameter. As the proportion of the probiotic solution increases to 40-90%, the intermolecular interaction is insufficient to maintain the continuity of the fiber, resulting in the fiber being unable to form normally and the fiber diameter being unevenly distributed. Considering that the probiotic mask provided herein needs to be quickly dissolved to release the probiotics to exert its skin care effect, a probiotic spinning solution with a probiotic solution ratio of 10% of the basic spinning solution should be selected, so that the prepared probiotic mask fiber has a smaller diameter, is evenly distributed, has a stronger ability to quickly dissolve to release the probiotics to exert its skin care effect, and the skin care effect is improved.

[0044] Correspondingly, the present application also provides the application of the above-mentioned probiotic facial mask in the field of skin care. The probiotic facial mask is a dry film before use, which is dry and waterless, flexible and tough, and easy to store. When using, only a small amount of small molecule water (lotion, toner, spray, essence, etc.) needs to be sprayed to achieve the effect of fiber dissolving in water, allowing the face to absorb it, and it will disappear by itself after use, achieving the effect of fiber dissolving in water, which is conducive to the rapid release of strains and the restoration of activity when exposed to water, thereby exerting skin care effects.

[0045] The probiotic facial mask provided by this application will be described in detail below with reference to embodiments and experimental examples.

[0046] Example 1

[0047] An embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask, and the preparation method includes the steps of preparing a probiotic spinning solution and needleless electrospinning.

[0048] Among them, the step of preparing the probiotic spinning solution includes: dissolving a water-soluble polymer in sterile deionized water and stirring at a high speed and low temperature of 600 r / min for 3-4 hours to obtain a basic spinning solution; adding the lyophilized powder of live strains to sterile deionized water and stirring at a high speed and constant temperature for a short time (the stirring speed is 500-800 r / min) to obtain a probiotic bacterial solution, and the environmental temperature should be kept at , and then adding the bacterial solution to the basic spinning solution and stirring at a low speed for a short time to obtain a probiotic spinning solution; stirring at a low speed for a short time can make the probiotics evenly present in the spinning solution.

[0049] The step of needleless electrospinning includes: under a nitrogen atmosphere to make the environment anaerobic or hypoxic during the electrospinning process, adding the probiotic spinning solution to the injection pump of the needleless electrospinning device, setting the operating parameters and environmental parameters of the solution electrospinning device, and setting the spinning temperature to , the environmental humidity is 10%RH, the positive voltage / current of the spinning voltage is 50.34 kV / 2.24 mA, and the negative voltage / negative current is , the liquid supply speed is 200 ml / h for electrospinning, and the coating speed of the electrospinning is 80 mm / s, the collection distance is 19 cm, the winding and unwinding speed is 0.3 m / min, the conveyor belt speed is 0.2 m / min, and a staggered and dense probiotic mask is spun on the receiving roller (the roller rotation speed is 80 r / min).

[0050] In Example 1, the ratio of the probiotic spinning solution is shown in Table 1:

[0051]

[0052] Table 1

[0053] Example 2

[0054] An embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask, and the difference between the preparation method and Example 1 is that the ratio of the probiotic spinning solution is different, and the ratio is shown in Table 2.

[0055]

[0056] Table 2

[0057] Example 3

[0058] The embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask. The difference between the preparation method and that of Example 1 lies in the different proportions of the probiotic spinning solution, as shown in Table 3.

[0059]

[0060] Table 3

[0061] Example 4

[0062] The embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask. The preparation method includes the steps of preparing a probiotic spinning solution and needleless electrospinning.

[0063] Among them, the step of preparing the probiotic spinning solution includes: dissolving polyvinyl alcohol with an alcoholysis degree of 89% in sterile deionized water, and stirring at a high speed and low temperature of 600 r / min for 3 - 4 hours to obtain a basic spinning solution with a concentration of 10% w / v; adding the freeze-dried powder of Lactobacillus paracasei into sterile deionized water and stirring at a high speed and constant temperature for a short time (stirring speed is 500 - 800 r / min) to obtain a Lactobacillus paracasei bacterial solution with a concentration of 500 CFU / ml. During the stirring process, the ambient temperature should be maintained at , and then mixing the Lactobacillus paracasei bacterial solution and the basic spinning solution in a ratio of 1:10, and stirring at a low speed for a short time to obtain a probiotic spinning solution; stirring at a low speed for a short time can make the probiotics evenly present in the spinning solution.

[0064] The step of needleless electrospinning includes: under a nitrogen atmosphere to make the environment anaerobic or hypoxic during the electrospinning process, adding the probiotic spinning solution into the injection pump of the needleless electrospinning device, setting the operating parameters and environmental parameters of the solution electrospinning device, and setting the electrospinning temperature to , the environmental humidity is 10% RH, the positive voltage / current of the electrospinning voltage is 50.34 kV / 2.24 mA, and the negative voltage / negative current is , the liquid supply speed is 200 ml / h to carry out electrospinning, and the coating speed of electrospinning is 80 mm / s, the collection distance is 19 cm, the winding and unwinding speed is 0.3 m / min, the conveyor belt speed is 0.2 m / min, and a staggered and dense probiotic mask (Lactobacillus paracasei / PVA = 0.1) is spun on the receiving roller (roller rotation speed is 80 r / min).

[0065] Example 5

[0066] The embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask. The difference between the preparation method and that of Example 4 lies in the different mixing ratios of the Lactobacillus paracasei bacterial solution and the basic spinning solution in the probiotic spinning solution.

[0067] In this embodiment, in the step of preparing the probiotic spinning solution, the Lactobacillus paracasei liquid and the base spinning solution are mixed in a ratio of 2:10; the prepared probiotic mask (Lactobacillus paracasei / PVA = 0.2).

[0068] Example 6

[0069] The embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask. The difference between the preparation method and Example 4 lies in that in the probiotic spinning solution, the mixing ratio of the Lactobacillus paracasei liquid and the base spinning solution is different.

[0070] In this embodiment, in the step of preparing the probiotic spinning solution, the Lactobacillus paracasei liquid and the base spinning solution are mixed in a ratio of 4:10; the prepared probiotic mask (Lactobacillus paracasei / PVA = 0.4).

[0071] Example 7

[0072] The embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask. The difference between the preparation method and Example 4 lies in that in the probiotic spinning solution, the mixing ratio of the Lactobacillus paracasei liquid and the base spinning solution is different.

[0073] In this embodiment, in the step of preparing the probiotic spinning solution, the Lactobacillus paracasei liquid and the base spinning solution are mixed in a ratio of 7:10; the prepared probiotic mask (Lactobacillus paracasei / PVA = 0.7).

[0074] Example 8

[0075] The embodiment of the present application provides a needleless electrospinning preparation method for a probiotic mask. The difference between the preparation method and Example 4 lies in that in the probiotic spinning solution, the mixing ratio of the Lactobacillus paracasei liquid and the base spinning solution is different.

[0076] In this embodiment, in the step of preparing the probiotic spinning solution, the Lactobacillus paracasei liquid and the base spinning solution are mixed in a ratio of 9:10; the prepared probiotic mask (Lactobacillus paracasei / PVA = 0.9).

[0077] Experimental Example 1

[0078] The experimental example of the present application performs structural characterization and performance testing on the probiotic masks provided in Examples 4-8.

[0079] Among them, the structural characterization test process of the probiotic mask provided in Examples 4-8 is as follows: the probiotic mask is cut and sampled in a targeted manner, and then gold sprayed using a plasma thin film sputtering device, and then detected using an electron microscope; in order to prevent the probiotic mask from falling off during the vacuum extraction process, a probiotic mask sample of appropriate size needs to be fixed on a metal sample table, and the fiber morphology magnified 1500 times is observed using an accelerating voltage of 5 kV; after shooting, 50 fibers are randomly selected for measurement using ImageJ software (ImageJ 1.54g), and the Origin software (Origin2021) is used to statistically analyze the diameter distribution of the spun fibers in the probiotic mask.

[0080] The results of characterization are as follows Figure 2-6 As shown, from Figure 2-6 It can be seen that when the proportion of the probiotic solution is 10-40% of the basic spinning solution, as the proportion of the probiotic solution increases, the diameter of the spun fiber in the probiotic mask gradually increases, and when the proportion of the probiotic solution increases from 40% to 90%, the fiber diameter gradually begins to decrease, but the diameter decreases slightly, and it is easy for the fiber to fail to form normally and the fiber diameter distribution is uneven; that is, when the ratio of the probiotic solution to the basic spinning solution is 4:10, the diameter of the spun fiber in the probiotic mask prepared is the largest, and when the ratio of the probiotic solution to the basic spinning solution is 9:10 , the diameter of the spun fibers in the prepared probiotic facial mask is large and unevenly distributed, while when the ratio of the probiotic solution to the basic spinning solution is 1:10, the spun fibers in the prepared probiotic facial mask are the smallest, and the distribution is concentrated with a small standard deviation; when the probiotic facial mask provided by the present application is used for skin care, considering that the spun fibers in the probiotic facial mask need to be dissolved before the probiotics can be effectively released to exert the skin care effect, smaller spun fibers may be easier to dissolve. Therefore, when preparing the probiotic facial mask, the ratio of the probiotic solution to the basic spinning solution should be selected as 1:10 to configure the probiotic spinning solution.

[0081] Furthermore, the present application also conducted a quick-dissolution test on the probiotic facial mask provided in Examples 4-8. The test process was as follows: the probiotic facial mask was cut into a rectangle, and then the probiotic facial mask sample was picked up with tweezers and placed in deionized water. In order to record the dissolution process of the probiotic facial mask, a camera was used to take pictures.

[0082] The instant dissolution effect test of the probiotic mask provided in Examples 4-8 is as follows Figure 8 As shown, from Figure 8It can be seen that when the proportion of the probiotic solution increases from 10% to 90%, the dissolution time will also be extended, which may be due to the influence of the diameter and distribution of the spun fibers in the probiotic mask; overall, however, the dissolution time of the probiotic mask provided in this application is not long, showing a quick dissolution effect; further considering that when consumers hope for the quick dissolution of the probiotic mask to rapidly release probiotics for skin care effects, when upstream cosmetics manufacturers prepare the probiotic mask, the ratio of the probiotic solution to the basic spinning solution should be 1:10 to prepare the probiotic spinning solution, but when consumers hope for the slow release of probiotics in the probiotic mask, when upstream cosmetics manufacturers prepare the probiotic mask, the ratio of the probiotic solution to the basic spinning solution should be 9:10 to prepare the probiotic spinning solution.

[0083] Furthermore, this application also conducted skin care effect tests on the probiotic masks provided in Examples 4-8. The test process includes:

[0084] (1) Measurement of the initial skin state: First, expose the target skin area to the air at room temperature ( ) for 10 minutes to ensure that the skin is in a natural state; then use an SK-I digital skin moisture detector to measure the water content and oil content of the skin in this area and record the initial data.

[0085] (2) Mask moisturizing performance test: First, apply the probiotic masks provided in Examples 4-8 and the mask provided in Comparative Example 1 to the skin test sites, then use a spray bottle filled with deionized water to spray the masks to quickly melt them and be absorbed by the skin, then gently massage the skin area to promote the absorption of the mask ingredients. After 10 minutes, use the instrument to measure the water content and oil content of the skin in this area again and record the data. Before each experiment, wait for a period of time to ensure that the skin returns to the initial state before conducting the next measurement.

[0086] (3) Control experiment: While performing step 2, select an adjacent skin area as a control: Spray deionized water on this area and gently massage it. After 10 minutes, use the instrument to measure the water content and oil content of the skin in this control area and record the data.

[0087] The test results are as Figure 9 shown, Figure 9 which shows the moisturizing performance test results of directly spraying ionic water on the skin and the probiotic masks provided in Examples 4-8; as Figure 9 can be seen, compared with directly spraying ionic water on the skin, applying the probiotic masks of Examples 4-8 of this application on the skin can significantly increase the water content of the skin and reduce the oil content of the skin, indicating that the probiotic masks provided in this application have better skin care effects of moisturizing and removing oil.

[0088] Furthermore, as Figure 9It can also be seen that there are differences in the skin care effects of the probiotic masks provided in Examples 4-8 of this application. The probiotic masks provided in Examples 6-7 have better moisturizing and oil-removing effects. After the probiotic mask provided in Example 6 is applied to the skin, the water content of the skin can be increased by 74% and the oil content can be reduced by 12%, showing better moisturizing and oil-removing performance. The probiotic mask provided in Example 7 can also show similar effects. Therefore, when upstream cosmetics manufacturers prepare probiotic masks and hope to achieve stronger moisturizing and oil-removing effects of the probiotic masks, the ratio of the probiotic solution to the basic spinning solution should be selected as 4:10 or 7:10 to prepare the probiotic spinning solution.

[0089] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A needleless electrospinning preparation method for a probiotic facial mask, characterized in that, It includes the following steps: The step of preparing a basic spinning solution, dissolving a water-soluble polymer in sterile ionized water to obtain a basic spinning solution; The step of preparing a probiotic solution, mixing probiotic freeze-dried powder and sterile ionized water evenly to obtain a probiotic solution; The step of preparing a probiotic spinning solution, mixing the probiotic solution with the basic spinning solution to obtain a probiotic spinning solution; The step of preparing a probiotic facial mask, adding the probiotic spinning solution into the injection pump of a needleless electrospinning device, starting the needleless electrospinning device to perform electrospinning to obtain a probiotic facial mask.

2. The needleless electrospinning preparation method of a probiotic mask according to claim 1, characterized in that, In the step of preparing the basic spinning solution, the water-soluble polymer is selected from at least one of polyethylene oxide, polyvinyl alcohol, and polyvinylpyrrolidone.

3. The needleless electrospinning preparation method of a probiotic facial mask according to claim 2, characterized in that, The water-soluble polymer is selected from polyvinyl alcohol with an alcoholysis degree of 87%-89%.

4. The needleless electrospinning preparation method of a probiotic facial mask according to claim 1, wherein In the step of preparing the probiotic solution, the probiotic freeze-dried powder is selected from at least one of Lactobacillus delbrueckii freeze-dried powder, Lactobacillus rhamnosus freeze-dried powder, and Lactobacillus paracasei freeze-dried powder.

5. The needleless electrospinning preparation method of a probiotic mask according to claim 1, characterized in that, In the step of preparing the probiotic spinning solution, calculated by mass, the probiotic spinning solution includes 1-5 parts by mass of probiotics, 4-10 parts by mass of water-soluble polymer, and 85-95 parts by mass of sterile ionized water.

6. The needleless electrospinning preparation method of a probiotic facial mask according to claim 1, characterized in that, In the step of preparing the probiotic spinning solution, calculated by volume, the volume ratio of the probiotic solution to the basic spinning solution in the probiotic spinning solution is: 1-9:10; The probiotic solution is selected from a probiotic solution of 100-1000 CFU / ml; The basic spinning solution is selected from a basic spinning solution of 5-20 w / v.

7. A needleless electrospinning preparation method of a probiotic facial mask according to claim 1, characterized in that In the step of preparing the probiotic facial mask, the liquid supply speed of the injection pump in the needleless electrospinning device is 100-1000 ml / h.

8. The needleless electrospinning preparation method of a probiotic facial mask according to claim 1, characterized in that, In the steps of preparing the probiotic facial mask, the positive voltage / current of the electrospinning is 50.34 kV / 2.24 mA, and the negative voltage / negative current is .

9. The needleless electrospinning preparation method of a probiotic facial mask according to claim 1, characterized in that In the step of preparing the probiotic facial mask, the coating speed of the electrospinning is 60-100 mm / s, the collection distance is 15-25 cm, the winding and unwinding speed is 0.1-0.5 m / min, and the conveyor belt speed is 0.1-0.5 m / min.

10. Application of a probiotic facial mask prepared by the needleless electrospinning preparation method according to any one of claims 1-9 in the field of skin care.

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