Pesticide composition as well as preparation method and application thereof

Abstract

The present invention relates to the technical field of pesticides, and provides a sterilization composition, the sterilization composition contains active components of pyrazol-ethyl carbamate and amino-oligosaccharin, and the weight percentage of the pyrazol-ethyl carbamate to the amino-oligosaccharin is 1: 10-30: 1. The invention further relates to application of the bactericidal composition to prevention and treatment of cucumber downy mildew and the like.

Description

[0001] This application is a divisional application of a patent for invention with the application number CN202310859413.4, the application date of July 13, 2023, and the invention title of a bactericidal composition containing picarbutrazox. Technical Field

[0002] The present invention belongs to the technical field of pesticides and relates to a bactericidal composition containing picarbutrazox and oligosaccharins, which is used for preventing and controlling cucumber downy mildew and the like. Background Art

[0003] Picarbutrazox is a carbamate fungicide developed by Nihon Soda Co., Ltd. Currently, the mechanism of action of picarbutrazox is not clear, and the Fungicide Resistance Action Committee (FRAC) classifies it as an independent class U17. Picarbutrazox has osmotic and therapeutic effects and is effective against oomycete diseases, such as Bremia, Pythium, Pseudoperonospora, and Phytophthora; it has a wide range of application crops, including soybeans, corn, rice, cucumbers, melons, watermelons, tomatoes, lettuce, broccoli, cabbages, Chinese cabbages, onions, radishes, spinach, potatoes, gourds, and lawns.

[0004] Oligosaccharins are a kind of low-toxic fungicides with disease-resistant effects extracted from microbial metabolism. Oligosaccharins can inhibit the growth of some pathogens, affect the germination of fungal spores, induce morphological variation of hyphae, and biochemical changes in spores. It can stimulate genes in plants to produce chitinase, glucanase, phytoalexin, and PR proteins with disease-resistant effects, and has a cell activation effect, which helps the recovery of damaged plants, promotes root growth and strong seedlings, enhances the stress resistance of crops, and promotes plant growth and development. Oligosaccharin solutions can be widely used to prevent and control diseases caused by viruses, bacteria, and fungi in fruit trees, vegetables, underground rhizomes, tobacco, Chinese medicinal materials, and grain and cotton crops.

[0005] There is no relevant report on the combined use of picarbutrazox and oligosaccharins in the prior art. Summary of the Invention

[0006] The purpose of the present invention is to provide a bactericidal composition with obvious synergistic effects, which can delay resistance and reduce the dosage.

[0007] To solve the above problems, the technical solution adopted by the present invention is:

[0008] A fungicidal composition comprising tetrazolium pyridine and amino oligosaccharide, wherein the active ingredients comprise tetrazolium pyridine and amino oligosaccharide, and the mass ratio of tetrazolium pyridine to amino oligosaccharide is 1:10 to 30:1. Preferably, the mass ratio of tetrazolium pyridine to amino oligosaccharide is 1:1 to 30:1. More preferably, the mass ratio of tetrazolium pyridine to amino oligosaccharide is 20:1.

[0009] The weight percentage of the active ingredient in the bactericidal composition is 5 to 80%, preferably 10 to 50%.

[0010] The bactericidal composition has a significant effect on preventing and controlling cucumber downy mildew, and is significantly better than the effect of a single agent.

[0011] The advantages of the present invention are that the combination of the two exhibits a significant synergistic effect within a certain range, can reduce the dosage and lower the cost; can delay resistance; is highly safe, and is environmentally friendly. Implementation Method

[0013] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific examples, but the present invention is by no means limited thereto. The percentages in the examples are all by weight.

[0014] Application Example 1: Indoor Toxicity Test of Tetrazolylpyramid and Oligosaccharin Complex against Cucumber Downy Mildew

[0015] Test materials:

[0016] Cucumber downy mildew (Pseudoperonospora cubensis); collected and identified in the field, and preserved in living culture indoors.

[0017] Test method:

[0018] Refer to the fungicide control test for cucumber downy mildew (pot culture method) specified in the "Guidelines for Indoor Pesticide Testing of Fungicides" (NY / T 1156.7-2006). When the cucumbers have 8 to 10 true leaves, select healthy, disease-free potted cucumber seedlings with similar growth patterns for the test. Sporulate a fresh sporangium suspension evenly onto the undersides of the cucumber seedling leaves. The seedlings are then incubated in an artificial climate chamber at 20 ± 2°C, a light-dark ratio of 12:12, and 90% relative humidity. After 24 hours, spray the stems and leaves with the fungicide (the concentration gradient is determined based on preliminary experiments). Once the blanks have fully developed disease, investigate the disease.

[0019] Grading standards:

[0020] Level 0: no lesions;

[0021] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0022] Level 3: The lesion area accounts for 6 - 10% of the entire leaf area;

[0023] Level 5: The lesion area accounts for 11 - 25% of the entire leaf area;

[0024] Level 7: The lesion area accounts for 26 - 50% of the entire leaf area;

[0025] Level 9: The lesion area accounts for more than 50% of the entire leaf area.

[0026] Disease index = ∑(number of diseased leaves at each level × relative level index) / (total number of leaves surveyed × highest level value) × 100

[0027] Control effect (%) = [(control disease index - treated disease index) / control disease index] × 100

[0028] Use DPS data processing software to calculate the test results, and respectively obtain the virulence regression equations, EC50 and EC90 of the single agent of the test agent and the mixed agents with different ratios, compare the synergistic effects, and calculate the co-toxicity coefficient (CTC) of the compound agent mixture according to the Sun Yunpei method. The calculation formula of the co-toxicity coefficient (CTC) is as follows:

[0029] Actual toxicity index (ATI) = (EC50 of standard agent / EC50 of test agent) × 100

[0030] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage content of agent A in the mixture + toxicity index of agent B × percentage content of agent B in the mixture

[0031] Co-toxicity coefficient (CTC) = (ATI / TTI) × 100

[0032] According to the classification standard of the co-toxicity coefficient (CTC), CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect.

[0033] When tetraazolopyrimidinate and oligosaccharins are compounded in the range of 1:l0 - 30:1, the co-toxicity coefficient (CTC) is greater than 120, showing a synergistic effect on cucumber downy mildew. Especially when the mass ratio of tetraazolopyrimidinate to oligosaccharins is 20:1, the compounding effect of the two is the best.

[0034] In summary, the compound bactericidal composition of tetraazolopyrimidinate and oligosaccharins described in the present invention has a significant synergistic effect on cucumber downy mildew, expands the bactericidal spectrum, can delay resistance, is safe for crops, can reduce the dosage of pesticides, and is safe for the environment.

Claims

1. A preparation method of a pesticide composition, characterized in that, Mix tebuconazole and oligosaccharins uniformly by dissolving them in a solvent.

2. The preparation method according to claim 1, characterized in that, The mass ratio of tebuconazole to oligosaccharins is 1:10 to 30:

1.

3. The preparation method according to claim 1, wherein, The mass ratio of tebuconazole to oligosaccharins is 20:

1.

4. The preparation method according to claim 1, characterized in that, Tebuconazole and oligosaccharins account for 5 to 80% of the total mass.

5. The preparation method according to claim 1, characterized in that, Tebuconazole and oligosaccharins account for 10 to 50% of the total mass.

6. A pesticide composition, characterized in that, Prepared by the preparation method according to any one of claims 1-5.

7. Use of the pesticide composition according to claim 6 in the preparation of a drug for controlling cucumber downy mildew.