Extract containing aloe polysaccharide
By extracting high-purity aloe polysaccharide from the whole leaves, gel or leaf skin, drugs for the treatment of constipation and colorectal damage were prepared, which solved the side effects of existing drugs and achieved safe and effective therapeutic effects.
Patent Information
- Application Number
- CN202510638603.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-19
- Publication Date
- 2025-08-01
AI Technical Summary
Existing constipation drugs often cause abdominal cramps and diarrhea. Long-term use will cause dependence and will not improve colorectal damage caused by constipation.
Aloe vera polysaccharide extract is used to extract high-purity aloe polysaccharide from the whole leaves, gel or leaf skin through hot water extraction, alcohol precipitation and dialysis. It is used to prepare drugs for the treatment of constipation and colorectal damage.
Effectively treat constipation, significantly improve colorectal damage, avoid the side effects of conventional drugs, and improve the therapeutic effect and safety.
Smart Images
Figure CN120392900A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to an extract containing aloe polysaccharide, belonging to the field of medicine. Background Art
[0002] Constipation is a common and persistent gastrointestinal disorder. The global combined prevalence rate is about 15%, with a higher prevalence in women and the elderly. Constipation is characterized by difficult defecation, small stool volume, a feeling of incomplete defecation or excessive defecation time, the need for assisted defecation, abdominal distension, and dry and sticky stools. Although constipation is not fatal, the persistence of its symptoms can cause great pain to patients and seriously affect their quality of life.
[0003] Current constipation medications mainly include "laxative" and "moistening" types of prokinetic drugs, motilin receptor agonists, etc. Such drugs often cause abdominal colic and explosive diarrhea, and long-term use can lead to dependence and habitual constipation.
[0004] Moreover, long-term constipation is accompanied by pathological changes in the colorectum. Long-term use of related laxatives usually exacerbates the relevant situation, and even the use of laxative drugs cannot improve it.
[0005] Aloe is a perennial evergreen herb of the genus Aloe in the family Asphodelaceae and is also a plant with both medicinal and edible uses. Aloe mainly contains various types of chemical components such as anthraquinones, anthrones, chromones, pyranones, and polysaccharides. Although aloe has the effect of treating constipation, it is generally considered that the small molecule compounds - anthraquinone components contained in it play a role in treating constipation. There are no relevant reports on the use of other parts and components of aloe for the treatment of constipation. Summary of the Invention
[0006] In order to solve the above technical problems, the present invention provides an extract containing aloe polysaccharide, and the extract is from one or more mixtures of whole aloe leaves, aloe gel, or aloe leaf skin.
[0007] Further, in the above extract containing aloe polysaccharide, the extract is preferably from whole aloe leaves.
[0008] Further, in the above extract containing aloe polysaccharide, the extract is prepared by the following method:
[0009] Aloe gel, whole aloe leaves or leaf skin are subjected to hot water extraction, the extraction liquids are combined and filtered, the filtrate is subjected to ethanol precipitation under reduced pressure, allowed to stand for precipitation, filtered, the precipitate is redissolved and then dialyzed, and the supernatant is obtained after centrifugation of the dialysate and dried to obtain the extract.
[0010] Further, in the above extract containing aloe polysaccharide, the solution for ethanol precipitation is ethanol, preferably a high-concentration ethanol aqueous solution. The high-concentration ethanol solution is an ethanol aqueous solution with a concentration of 70 - 98%.
[0011] The precipitation is preferably redissolved in water.
[0012] Furthermore, in the above-mentioned extract containing aloe polysaccharide, the dialysis specification is 2000 - 4000 Da. Generally, it is judged according to the molecular weight of the crude polysaccharide. Generally, polysaccharides have a molecular weight higher than 2000 Da. In order to remove impurities as much as possible, a dialysis specification of 2500 - 3500 Da is preferred, and a uniform component is easily obtained.
[0013] Furthermore, the present invention provides an application of the above-mentioned extract of aloe polysaccharide in the preparation of a medicament for treating constipation.
[0014] Furthermore, the present invention provides an application of the above-mentioned extract of aloe polysaccharide in the preparation of a medicament for treating colorectal injury caused by constipation.
[0015] The ethanol solution described herein is an aqueous ethanol solution.
[0016] This article has the following technical advantages:
[0017] It provides an extract containing aloe polysaccharide, which is simple to prepare, and the contained saccharide components are stable. The whole aloe leaf is preferably used, which can solve the problem that conventional aloe extracts cannot treat constipation. More significantly, it can effectively solve the colorectal injury caused by constipation that cannot be solved by conventional aloe extracts. Description of the Drawings
[0018] Figure 1 : H&E staining of the colorectum of loperamide-induced constipated rats by aloe polysaccharide (×100); where Ⅰ: Group C, Ⅱ: Group M, Ⅲ: Crude aloe polysaccharide group. Detailed Embodiments
[0019] Example 1: Take 20 kg of whole aloe leaves, add 20 times distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, it is precipitated with high-concentration ethanol (95% ethanol), and the final volume fraction of the precipitation is 80%. Let it stand at 4 °C for 12 h, filter by vacuum suction. After the precipitation is redissolved, it is dialyzed with a 3500 Da dialysis bag for 72 h. The dialysis solution is centrifuged to take the supernatant, and freeze-dried to obtain 90 g of whole leaf crude polysaccharide (AWPs). It is verified by polysaccharide content determination experiments such as the phenol-sulfuric acid method that the obtained product is crude aloe polysaccharide.
[0020] Example 2: Take 10 kg of aloe vera gel, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), with the final volume fraction of the precipitate being 80%. Let it stand at 4 °C for 12 h, filter by vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate and take the supernatant, then freeze-dry to obtain 38 g of crude polysaccharide from whole aloe leaves (AWPs).
[0021] Example 3: Take 20 kg of aloe vera leaf peel, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), with the final volume fraction of the precipitate being 80%. Let it stand at 4 °C for 12 h, filter by vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate and take the supernatant, then freeze-dry to obtain 74 g of crude polysaccharide from whole aloe leaves (AWPs).
[0022] Example 4: Take 20 kg of whole aloe vera leaves, add 10 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), with the final volume fraction of the precipitate being 75%. Let it stand at 4 °C for 12 h, filter by vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate and take the supernatant, then freeze-dry to obtain 71 g of crude polysaccharide from whole aloe leaves (AWPs).
[0023] Example 5: Take 20 kg of whole aloe vera leaves, add 30 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), with the final volume fraction of the precipitate being 80%. Let it stand at 4 °C for 12 h, filter by vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate and take the supernatant, then freeze-dry to obtain 88 g of crude polysaccharide from whole aloe leaves (AWPs).
[0024] Example 6: Take 20 kg of whole aloe vera leaves, add 10 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), with the final volume fraction of the precipitate being 85%. Let it stand at 4 °C for 12 h, filter by vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate and take the supernatant, then freeze-dry to obtain 68 g of crude polysaccharide from whole aloe leaves (AWPs).
[0025] Example 7: Take 20 kg of whole aloe leaves, add 30 times the amount of distilled water, and perform hot water extraction (80 - 90 °C) for 2 h. Extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, use high-concentration ethanol (95% ethanol) for alcohol precipitation. The final volume fraction of alcohol precipitation is 70%. Let it stand at 4 °C for 12 h, then perform vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 80 g of crude polysaccharide from whole leaves (AWPs).
[0026] Example 8: Take 10 kg of aloe gel, add 20 times the amount of distilled water, and perform hot water extraction (80 - 90 °C) for 2 h. Extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, use high-concentration ethanol (90% ethanol) for alcohol precipitation. The final volume fraction of alcohol precipitation is 70%. Let it stand at 4 °C for 12 h, then perform vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 35 g of crude polysaccharide from whole leaves (AWPs).
[0027] Example 9: Take 10 kg of aloe gel, add 20 times the amount of distilled water, and perform hot water extraction (80 - 90 °C) for 2 h. Extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, use high-concentration ethanol (90% ethanol) for alcohol precipitation. The final volume fraction of alcohol precipitation is 75%. Let it stand at 4 °C for 12 h, then perform vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 37 g of crude polysaccharide from whole leaves (AWPs).
[0028] Example 10: Take 20 kg of aloe leaf skin, add 20 times the amount of distilled water, and perform hot water extraction (80 - 90 °C) for 2 h. Extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, use high-concentration ethanol (90% ethanol) for alcohol precipitation. The final volume fraction of alcohol precipitation is 70%. Let it stand at 4 °C for 12 h, then perform vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 70 g of crude polysaccharide from whole leaves (AWPs).
[0029] Example 11: Take 20 kg of aloe leaf skin, add 20 times the amount of distilled water, and perform hot water extraction (80 - 90 °C) for 2 h. Extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, use high-concentration ethanol (95% ethanol) for alcohol precipitation. The final volume fraction of alcohol precipitation is 85%. Let it stand at 4 °C for 12 h, then perform vacuum filtration. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 69 g of crude polysaccharide from whole leaves (AWPs).
[0030] Example 12: Take 20 kg of whole aloe leaves, add 10 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), and the final volume fraction of precipitation is 85%. Let it stand at 4 °C for 6 h, filter by vacuum suction. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 60 g of crude polysaccharides from whole leaves (AWPs).
[0031] Example 13: Take 20 kg of whole aloe leaves, add 30 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), and the final volume fraction of precipitation is 70%. Let it stand at 4 °C for 24 h, filter by vacuum suction. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 85 g of crude polysaccharides from whole leaves (AWPs).
[0032] Example 14: Take 20 kg of aloe leaf skin, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), and the final volume fraction of precipitation is 85%. Let it stand at 4 °C for 6 h, filter by vacuum suction. After the precipitate is redissolved, dialyze it with a 3500 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 59 g of crude polysaccharides from whole leaves (AWPs).
[0033] Example 15: Take 20 kg of whole aloe leaves, add 10 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (95% ethanol), and the final volume fraction of precipitation is 85%. Let it stand at 4 °C for 6 h, filter by vacuum suction. After the precipitate is redissolved, dialyze it with a 4000 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 73 g of crude polysaccharides from whole leaves (AWPs).
[0034] Example 16: Take 10 kg of aloe gel, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate it with high-concentration ethanol (90% ethanol), and the final volume fraction of precipitation is 70%. Let it stand at 4 °C for 12 h, filter by vacuum suction. After the precipitate is redissolved, dialyze it with a 2000 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry it to obtain 35 g of crude polysaccharides from whole leaves (AWPs).
[0035] Example 17: Take 10 kg of aloe vera gel, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate with high-concentration ethanol (90% ethanol), and the final volume fraction of precipitation is 70%. Let it stand at 4 °C for 12 h, then filter by vacuum. After the precipitate is redissolved, dialyze with a 3000 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry to obtain 30 g of crude polysaccharide from whole aloe leaves (AWPs).
[0036] Example 18: Take 20 kg of aloe vera leaf peel, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate with high-concentration ethanol (95% ethanol), and the final volume fraction of precipitation is 80%. Let it stand at 4 °C for 12 h, then filter by vacuum. After the precipitate is redissolved, dialyze with a 3000 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry to obtain 71 g of crude polysaccharide from whole aloe leaves (AWPs).
[0037] Example 19: Take 20 kg of aloe vera leaf peel, add 20 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate with high-concentration ethanol (95% ethanol), and the final volume fraction of precipitation is 80%. Let it stand at 4 °C for 12 h, then filter by vacuum. After the precipitate is redissolved, dialyze with a 4000 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry to obtain 58 g of crude polysaccharide from whole aloe leaves (AWPs).
[0038] Example 20: Take 20 kg of whole aloe vera leaves, add 10 times the amount of distilled water, extract with hot water (80 - 90 °C) for 2 h, extract twice, combine the extracts and filter. After the filtrate is concentrated under reduced pressure to a certain volume, precipitate with high-concentration ethanol (90% ethanol), and the final volume fraction of precipitation is 80%. Let it stand at 4 °C for 6 h, then filter by vacuum. After the precipitate is redissolved, dialyze with a 3000 Da dialysis bag for 72 h. Centrifuge the dialysate to obtain the supernatant, and freeze-dry to obtain 55 g of crude polysaccharide from whole aloe leaves (AWPs).
[0039] Example 21
[0040] Experiment on the anti-constipation effect of crude polysaccharide from whole aloe vera leaves
[0041] 1 Materials and animal grouping
[0042] 1.1 Experimental animals
[0043] Male Sprague-Dawley (SD) rats, SPF grade, weighing 180 ± 20 g, were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China), and the license number was: SYXK (Jing) 2023-0008. All SD rats were housed in the Animal Experiment Center of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences. The experimental protocol has been approved by the Animal Experiment Ethics Committee of the Institute of Medicinal Plant Development, Peking Union Medical College. The SD rats were allowed free access to food and water and were housed in an environment with a room temperature of 20-25°C, humidity of 50 ± 10%, and a light period of 8:00-18:00. The experiment began after 7 days of adaptation.
[0044] 1.2 Experimental Instruments and Reagents
[0045] AE100 electronic balance (Mettler-Toledo Instruments Shanghai Co., Ltd.); T520 electronic balance (Xiangyi, Changsha, Hunan); Loperamide was purchased from Sigma-Aldrich Company, USA; Arabic gum was purchased from Beijing Solarbio Science & Technology Co., Ltd.; 4% paraformaldehyde general tissue fixative was purchased from Beijing Labgic Technology Co., Ltd.; ELISA kits for rat 5-hydroxytryptamine (5-HT), motilin (MTL), somatostatin (SST), and vasoactive intestinal peptide (VIP) were all purchased from Wuhan Elabscience Co., Ltd.; 3500 Da dialysis bag (MD44-5M) was purchased from MYM Company, USA; reagents such as absolute ethanol, eosin Y (water-soluble), hydrochloric acid, and neutral gum were all purchased from Sinopharm Chemical Reagent Co., Ltd.
[0046] 1.3 Experimental Samples
[0047] See Examples 1 to 20.
[0048] 1.4 Sample Concentration Setting
[0049] The concentration of crude aloe polysaccharide was 75 mg / mL.
[0050] 1.5 Data Processing and Statistical Analysis
[0051] Graphpad Prism 5 (San Diego, USA) software was used to compare the significant differences between groups. The statistical analysis results were expressed as mean ± standard deviation (Mean ± SD). One-way ANOVA was used for comparison among multiple groups of data, and p < 0.05 indicated that the difference was statistically significant.
[0052] 2 Experimental Methods
[0053] The rats in the constipation model group (Group M) were intragastrically administered with 3 mg / kg of loperamide solution twice a day (at 7:00 and 19:00) for 14 consecutive days to establish the model. The dosage volume of loperamide was calculated based on 0.3 mL / 100 g of rat body weight; the rats in the blank control group (Group C) were intragastrically administered with an equal volume of water. After 14 days of modeling, administration began. Normal rats were given distilled water, and constipated rats were intragastrically administered with 300 mg / kg (polysaccharide / body weight) of crude aloe polysaccharide solution for 14 consecutive days. During the treatment period, the model rats continued to be given loperamide. After the experiment, the small intestine transit rate was measured by intragastric administration of activated carbon, and samples such as rat plasma / serum, colon-rectum, and feces were taken.
[0054] 3 Effects of Aloe Polysaccharide on General Signs and Gastrointestinal Transit of Constipated Rats
[0055] As shown in Table 1, compared with the blank control group (C), the fecal water content, small intestine propulsion rate, and number of feces excreted in 12 h of the rats in the model group (M) were significantly decreased. After 14 days of treatment with aloe polysaccharide, the fecal water content, body weight, small intestine propulsion rate, and number of feces excreted in 12 h of the constipated rats showed a significant increasing trend compared with the model group, and the effect of Example 1 was the best.
[0056] Table 1 Effects of Aloe Polysaccharide on General Signs and Gastrointestinal Transit of Constipated Rats (n = 6)
[0057]
[0058]
[0059] Note: Compared with the normal control group, ## P < 0.05, # P < 0.01; compared with the model group, ** P < 0.05, * P < 0.01.
[0060] 4 Effects of Aloe Polysaccharide on Neurotransmitters of Constipated Rats
[0061] As shown in Table 2, compared with the normal control group (C), the excitatory neurotransmitters MTL and 5-HT of the rats in the model group (M) were significantly decreased. After 14 days of treatment with crude aloe polysaccharide, compared with the model group, the levels of MTL and 5-HT in the rats of the aloe polysaccharide group were significantly increased. Compared with the normal group, the levels of the inhibitory neurotransmitters SST and VIP in the model group were significantly increased. After 14 days of treatment with crude aloe polysaccharide, the levels of the inhibitory neurotransmitters SST and VIP in the constipated rats were significantly decreased, and the improvement effect of Example 1 was the most significant.
[0062] Table 2 Effects of Aloe Polysaccharide on Neurotransmitters MTL, 5-HT, SST, VIP of Constipated Rats (n = 6)
[0063]
[0064]
[0065] Note: Compared with the normal control group, ## P < 0.05, # P < 0.01; compared with the model group, ** P < 0.05, * P < 0.01.
[0066] 5 Effect of Aloe Polysaccharide on Colorectal Pathological Morphology in Constipated Rats
[0067] H&E stained sections of colorectal tissues of rats in each group were observed under a microscope. The results showed that the colorectal tissue structure of normal rats (Group C) was clear, the mucosa was intact, the crypts were arranged neatly, and no obvious pathological changes were observed. However, in the model rats (Group M), the colonic mucosal epithelium was severely damaged, the crypts were arranged disorderly, the number of large intestinal glands and goblet cells was significantly reduced, and a large number of inflammatory cells infiltrated. After 14 days of treatment with crude aloe polysaccharide, the colonic lesions of rats in each treatment group were improved to varying degrees. Among them, the improvement of the colorectal tissue lesions in Example 1 was the most significant, as shown in Figure 1 .
Claims
1. An extract containing aloe polysaccharide, characterized in that, The extract is derived from one or more mixtures of whole aloe leaves, aloe gel or aloe leaf rind.
2. The extract of aloe polysaccharide according to claim 1, wherein The extract is preferably derived from whole aloe leaves.
3. The extract of aloe polysaccharide according to claim 1, wherein, The extract is obtained by the following method: Aloe gel, whole aloe leaves or leaf rind are subjected to hot water extraction, the extraction liquids are combined and filtered, the filtrate is subjected to alcohol precipitation under reduced pressure, allowed to stand for precipitation, filtered, redissolved after precipitation, dialyzed, the supernatant is obtained after centrifugation of the dialysate, and dried to obtain the extract.
4. The extract of aloe polysaccharide according to claim 4, characterized in that, The solution for alcohol precipitation is ethanol, preferably an ethanol aqueous solution with a volume concentration of 70-98%.
5. The extract of aloe polysaccharide according to claim 4, wherein The specification of the dialysis is 2000-4000Da, preferably 2500-3500Da.
6. Use of the extract of aloe polysaccharide according to claim 1 in the preparation of a medicament for treating constipation.
7. Use of the extract of aloe polysaccharide according to claim 1 in the preparation of a medicament for treating colorectal injury caused by constipation.