Preparation and application of sixteen-component medicinal and edible homologous prescription for treating Alzheimer's disease and depression

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, and particularly discloses preparation and application of a sixteen-component medicine-food homologous prescription for treating Alzheimer's disease and depression through intelligence development and depression, and the preparation method comprises the following steps: S1, weighing the medicinal materials according to the prescription dosage, cleaning, selecting, drying and crushing to 50-100 meshes; s2, adding water into the medicinal materials, decocting for 1-3 times, decocting for 0.5-2 hours each time, combining filtrates at 60 DEG C, and concentrating until the relative density is 1.10-1.30, wherein the amount of added water is 5-15 times of the weight of the medicinal materials; s3, treating the concentrated solution by an alcohol precipitation method, standing, and recovering ethanol to obtain an extract; s4, mixing the extract with auxiliary materials to prepare one of granules, capsules or tablets; according to the traditional Chinese medicine composition disclosed by the invention, through a multi-target synergistic effect, cognitive dysfunction and mood disorder of patients with senile dementia and depression are remarkably improved. The tonifying medicinal materials such as the astragalus membranaceus and the rhizoma polygonati in the formula can enhance cerebral blood flow, inhibit beta amyloid protein deposition and tau protein phosphorylation and delay neurodegenerative diseases.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to the preparation and application of a medicine and food homologous formula of Yizhi Jieyu Shiliuweito treat senile dementia and depression. Background Art

[0002] Senile dementia is a neurodegenerative disease with progressive cognitive impairment as the core. The core pathological features include senile plaques formed by the deposition of β-amyloid protein, neurofibrillary tangles caused by abnormal phosphorylation of tau protein, as well as neuronal loss and synaptic dysfunction. The clinical manifestations are memory loss, disorientation, impaired language function, etc., and emotional abnormalities and personality changes may occur in the late stage. Depression is an emotional disorder with persistent low mood and loss of interest as the core features, accompanied by cognitive impairment, somatic symptoms and negative thinking. Its pathogenesis involves multidimensional factors such as imbalance of monoamine neurotransmitters, impaired neural plasticity, hyperactivity of the HPA axis and immune inflammatory responses.

[0003] Currently, the therapeutic drugs for AD can only relieve symptoms in the short term, cannot prevent the progression of the disease, and are prone to cause gastrointestinal side effects and drug tolerance when used for a long time. The standardized chemotherapy regimen for depression has problems such as delayed onset, insufficient response in some patients, and high recurrence rate after drug withdrawal. Summary of the Invention

[0004] The purpose of the present invention is to provide the preparation and application of a medicine and food homologous formula of Yizhi Jieyu Shiliuweito treat senile dementia and depression, so as to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solutions:

[0006] A medicine and food homologous formula of Yizhi Jieyu Shiliuweito treat senile dementia and depression, comprising the following components in parts by weight:

[0007] Astragalus membranaceus 15 - 25 g, Polygonatum sibiricum 10 - 20 g, Rehmannia glutinosa 8 - 15 g, Salvia miltiorrhiza 8 - 15 g, Ligusticum chuanxiong 8 - 12 g, Angelica dahurica 3 - 9 g, Polygala tenuifolia 3 - 9 g, Gastrodia elata 8 - 12 g, Poria cocos 10 - 20 g, Ziziphus jujuba var. spinosa 8 - 12 g, Haliotis diversicolor 10 - 15 g, Glycyrrhiza uralensis 3 - 9 g.

[0008] Preferably, it further comprises Fritillaria thunbergii 6 - 12 g, Paeonia lactiflora 5 - 10 g, Atractylodes macrocephala 10 - 15 g, Alisma orientale 8 - 12 g.

[0009] A preparation method of a medicine and food homologous formula of Yizhi Jieyu Shiliuweito treat senile dementia and depression, comprising the following steps:

[0010] S1. Weigh each medicinal material according to the prescription dose, perform cleaning, drying and pulverize it to 50 - 100 meshes;

[0011] S2. Add water to the medicinal materials and decoct them 1 - 3 times. The decoction time for each time is 0.5 - 2 hours, and the water addition amount is 5 - 15 times the weight of the medicinal materials. Combine the filtrates at 60°C and concentrate to a relative density of 1.10 - 1.30.

[0012] S3. Treat the concentrated solution by alcohol precipitation method, let it stand, and then recover ethanol to obtain an extract.

[0013] S4. Mix the extract with excipients to make one of granules, capsules or tablets.

[0014] Preferably, in step S3, the final concentration of ethanol is 60%, and the standing time is 24 hours.

[0015] Use of the traditional Chinese medicine composition as described above in the preparation of a drug for improving cognitive function, regulating mood and inhibiting neurodegenerative diseases.

[0016] Compared with the prior art, the beneficial effects of the present invention are:

[0017] (1) Through multi - target synergistic effects, significantly improve the cognitive function disorders and mood disorders of patients with Alzheimer's disease and depression. In its formula, tonifying medicinal materials such as Astragalus membranaceus and Polygonatum sibiricum can enhance cerebral blood flow, inhibit the deposition of β - amyloid protein and the phosphorylation of tau protein, and delay neurodegenerative diseases; promoting blood circulation and removing stasis components such as Salvia miltiorrhiza and Ligusticum chuanxiong can reduce neuroinflammation and improve microcirculation through anti - inflammatory and antioxidant mechanisms; tranquilizing drugs such as Polygala tenuifolia and Ziziphus jujuba var. spinosa regulate the levels of 5 - hydroxytryptamine and brain - derived neurotrophic factor, and restore synaptic plasticity.

[0018] (2) This composition is based on medicinal and edible homologous medicinal materials, with high safety and suitable for long - term use. Compared with traditional chemical drugs, its multi - target action mechanism can simultaneously intervene in the comorbid pathology of AD and depression, has a wide range of clinical indications, can be flexibly added or subtracted according to syndromes, and has both precise curative effect and flexible medication. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] Example 1:

[0022] Please refer to Figure 1As shown, the sixteen - flavor prescription for promoting intelligence and relieving depression, which has both medicinal and edible properties for treating senile dementia and depression, comprises the following components in parts by weight:

[0023] Astragalus membranaceus 20g, Polygonatum sibiricum 15g, Rehmannia glutinosa (prepared) 12g, Salvia miltiorrhiza 12g, Ligusticum chuanxiong 10g, Angelica dahurica 6g, Polygala tenuifolia 6g, Gastrodia elata 10g, Poria cocos 15g, Ziziphus jujuba var. spinosa 10g, Haliotis diversicolor 12g, Licorice 6g.

[0024] The preparation method of the sixteen - flavor prescription for promoting intelligence and relieving depression, which has both medicinal and edible properties for treating senile dementia and depression, comprises the following steps:

[0025] S1. Weigh each medicinal material according to the prescription dosage, select and clean, dry and pulverize it to 80 meshes;

[0026] S2. First decoction: Add purified water 10 times the total weight of the medicinal materials, bring to a boil and keep slightly boiling for 1.5 hours, then filter;

[0027] Second decoction: Add 8 times the amount of purified water to the residue, bring to a boil for 1 hour, then filter;

[0028] Combine the two filtrates, concentrate under reduced pressure to a relative density of 1.20 at 60 °C;

[0029] S3. Add ethanol to the concentrated liquid to a final concentration of 60%, let it stand for 24 hours, take the supernatant and recover the ethanol to obtain an extract;

[0030] S4. Mix the extract with excipients to make one of granules, capsules or tablets;

[0031] Mix the extract with microcrystalline cellulose in a ratio of 1:1, granulate and dry, and package into 5 - g bags each.

[0032] Example 2:

[0033] The difference from Example 1 is that Fritillaria thunbergii 9g and Paeonia lactiflora 8g are added to the basic formula of Example 1;

[0034] [[ID=~36]]The preparation method is as follows: Treat the medicinal materials according to the steps of Example 1; The decoction, concentration and ethanol precipitation processes are the same as those in Example 1; Mix the extract with microcrystalline cellulose (excipient) in a weight ratio of 1:1.5, granulate and then fill it into capsule shells, with each capsule containing 3g of crude drug;

[0035] It is applicable to patients with the pattern of internal disturbance of phlegm - heat, 3 times a day, 2 capsules each time.

[0036] Example 3:

[0037] The difference from Example 1 is that Atractylodes macrocephala 12g and Alisma orientale 10g are added to the basic formula of Example 1;

[0038] The preparation method is as follows: process the medicinal materials according to the steps of Example 1; the decocting, concentrating, and ethanol precipitation processes are the same as those in Example 1; the extract is mixed with starch and magnesium stearate (auxiliary materials) in a weight ratio of 1:1:0.02, and pressed into tablets, with each tablet containing 2.5 g of crude drug content;

[0039] It is applicable to patients with the pattern of spleen deficiency and dampness exuberance, 2 times a day, 2 tablets each time.

[0040] Example 4:

[0041] The difference from Example 1 is that after the medicinal materials are sorted, they are directly decocted twice with water (the amount of water is the same as in Example 1); the filtrates are combined, without concentration or ethanol precipitation, and divided into decoctions, with each dose being 200 mL;

[0042] Take 1 dose per day, warm it and take it twice, in the morning and evening, and take it continuously for 4 weeks.

[0043] Experimental example:

[0044] Select SD rats (n = 10 / group) to receive 4 weeks of CUMS (fasting, swimming in ice water, etc.) to induce depressive-like behaviors; set up a normal group: no stress + normal saline; a model group: CUMS + normal saline; a traditional Chinese medicine group: CUMS + the granule of Example 1 (1 g / kg·d); a fluoxetine group: CUMS + fluoxetine (10 mg / kg·d);

[0045] Conduct behavioral tests, including the forced swimming test (FST) and the sucrose preference test (SPT), record the immobility time and calculate the sucrose consumption rate, and at the same time, use the ELISA method to measure the levels of 5-HT and BDNF in the hippocampus. The specific experimental data are as follows:

[0046]

[0047] As can be seen from the above, the traditional Chinese medicine group significantly reduces the immobility time, increases the sucrose preference and neurotransmitter levels (p < 0.01, *p < 0.001 vs. the model group).

[0048] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A medicated diet formula of Yizhi Jieyu Sixteen Ingredients for treating senile dementia and depression, characterized in that, Comprising the following components in parts by weight: Astragalus membranaceus 15 - 25 g, Polygonatum sibiricum 10 - 20 g, Rehmannia glutinosa 8 - 15 g, Salvia miltiorrhiza 8 - 15 g, Ligusticum chuanxiong 8 - 12 g, Angelica dahurica 3 - 9 g, Polygala tenuifolia 3 - 9 g, Gastrodia elata 8 - 12 g, Poria cocos 10 - 20 g, Ziziphus jujuba var. spinosa 8 - 12 g, Haliotis diversicolor 10 - 15 g, Glycyrrhiza uralensis 3 - 9 g.

2. The sixteen-ingredient prescription for promoting intelligence and relieving depression, which has both medicinal and edible properties and is used to treat Alzheimer's disease and depression, as claimed in claim 1, is characterized in that: Also comprising Fritillaria thunbergii 6 - 12 g, Paeonia lactiflora 5 - 10 g, Atractylodes macrocephala 10 - 15 g, Alisma orientale 8 - 12 g.

3. Preparation method of a medicated diet formula with homologous medicine and food for treating senile dementia and depression by Yizhi Jieyu Sixteen Flavors, characterized in that, Comprising the following steps: S1. Weigh each medicinal material according to the prescription dosage, perform cleaning, drying and pulverize to 50 - 100 meshes; S2. Decoct the medicinal materials with water for 1 - 3 times, each decocting time is 0.5 - 2 hours, the water addition amount is 5 - 15 times the weight of the medicinal materials, combine the filtrates at 60 °C and concentrate to a relative density of 1.10 - 1.30; S3. Treat the concentrated solution by alcohol precipitation method, recover ethanol after standing, to obtain an extract; S4. Mix the extract with excipients to prepare one of granules, capsules or tablets.

4. The preparation method of the sixteen-ingredient medicated diet formula for treating senile dementia and depression with the functions of strengthening the mind and relieving depression according to claim 3, characterized in that: In the said S3, the final concentration of ethanol is 60%, and the standing time is 24 hours.

5. Use of the traditional Chinese medicine composition according to claim 3 in the preparation of a drug for improving cognitive function, regulating mood and inhibiting neurodegenerative diseases.

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