Functional additive and feed for preventing and treating mycoplasma synoviae leg disease of broiler breeders

Abstract

The invention discloses a functional additive and feed for preventing and treating mycoplasma synoviae leg disease of broiler breeders. The functional additive is prepared from the following components in percentage by weight: 4-8% of cicada slough immunoactive peptide; 4%-8% of curcumin; 2%-4% of bacillus subtilis; 2%-6% of xylan compound enzyme; 14%-16% of chelated organic trace elements; 1.5%-2% of 25 hydroxy vitamin D; 10%-16% of sodium butyrate; the total percentage is 100%. When the functional additive for preventing and treating the mycoplasma synoviae leg disease of the broiler breeders is added into feed, the MS infection rate can be effectively prevented and reduced to 5% or below.

Description

Technical Field

[0001] The present invention relates to the technical field of feed, in particular to a functional additive and feed for preventing and treating mycoplasma synoviae leg disease in broiler breeders. Background Art

[0002] Mycoplasma synoviae leg disease is a common disease in broiler breeders, causing lameness, joint swelling, and decreased egg production, resulting in severe economic losses. Mycoplasma synoviae infection (MS) is caused by the parasitic bacterium Mycoplasma synoviae, with bursitis as the typical lesion. This disease leads to joint lesions in poultry, resulting in decreased egg production, and is vertically transmitted. Bursitis is the typical lesion that causes leg joint disease in poultry, characterized by swelling of the tarsal and wing joints, exudation of a yellow, viscous fluid, and later caseation. Mycoplasma synoviae is the primary pathogen of poultry joint disease, known as Mycoplasma synoviae leg disease. Mycoplasma synoviae (MS) belongs to the class Mollicutes, lacks a cell wall, and can pass through bacterial filters. It is an obligate parasite, primarily infecting the joints, synovium, and respiratory systems of chickens and turkeys, making it a significant pathogen in the poultry industry. Traditional prevention and treatment relies on antibiotics (such as tylosin), but these can easily lead to drug resistance, residues, and food safety issues. Existing functional additives often focus on a single effect (such as immune enhancement or anti-inflammatory effects), lacking comprehensive solutions that synergistically regulate MS pathogens and host immunity.

[0003] (I) Research progress on Mycoplasma synoviae leg disease: 1. Core characteristics of Mycoplasma synoviae disease (MS): (1) Typical lesion sites: Bursitis: The tarsal joints and wing joints are swollen, exuding yellow viscous fluid, which becomes caseous in the later stage.

[0004] Respiratory symptoms: cough, air sac inflammation.

[0005] (2) Vertical transmission characteristics: MS can be transmitted vertically through eggs.

[0006] 2. Clinical symptoms and diagnostic points: (1) Typical leg / joint symptoms: Acute phase: The tarsal joints and toe joints are swollen (in the shape of a steamed bun), with a sense of fluctuation on palpation, the joint cavity is filled with yellow sticky or purulent exudates, and the sick chickens are lame, paralyzed, or have one leg curled up.

[0007] Chronic stage: The exudate gradually becomes caseous (white or grayish yellow), the articular cartilage erodes, and the sternal synovial bursa cyst (causing "keel cyst") is formed. In severe cases, it is difficult to stand, and the elimination rate can reach 15%-30%.

[0008] (2) Egg-laying related symptoms: The egg production rate of breeder hens decreases by 5%-20%, the eggshell surface becomes rough (gravel-like protrusions), and the embryo death rate increases in the late incubation period (due to vertical transmission).

[0009] (3) Other systemic symptoms: Respiratory symptoms: mild cough and runny nose (easily confused with bronchitis), but no severe air sac inflammation (different from MG infection).

[0010] Systemic wasting: The comb becomes pale and shrunken, and the weight is reduced (the uniformity is reduced by more than 20%).

[0011] 3. Laboratory diagnostic methods: Pathogen detection: Joint fluid PCR (targeting the vlhA gene of MS) or culture (requires special culture medium and is time-consuming).

[0012] Serological testing: ELISA to detect antibodies (the positive rate increases significantly 2-3 weeks after infection).

[0013] 4. Causes and transmission routes: (1) Core pathogens: Mycoplasma Synoviae (MS): It has no cell wall, is sensitive to antibiotics but easily resistant, and can lurk in chickens for a long time.

[0014] (2) Transmission routes: Vertical transmission: The infection rate of hatching eggs can reach 30%-70%, and chicks can become sick at one week of age.

[0015] Horizontal transmission: Transmission through respiratory secretions, feces, and cross-contamination of personnel / equipment. Poor ventilation in chicken houses (ammonia concentration > 20 ppm) significantly aggravates the infection.

[0016] (3) Predisposing factors: Environmental stress: sudden temperature changes, high-density breeding, and frequent immunizations (such as Newcastle disease live vaccine irritating the respiratory mucosa).

[0017] Secondary infection: The mortality rate increases when mixed with Escherichia coli and viral arthritis (REO).

[0018] 5. Incidence: (1) Impact of breeding management: In well-managed flocks, the incidence rate is usually 5%-10% and the symptoms are mild.

[0019] Poor management or mixed infection: The incidence rate can reach 30%-50%, especially when sanitary conditions are poor or immunosuppressive diseases (such as infectious bursal disease) are present.

[0020] (2) Age and breed differences: Stage differences: The incidence rate is relatively high in broilers at 3 - 6 weeks of age, laying hens at 12 - 20 weeks of age, and breeding hens during the laying period.

[0021] Breed differences: Fast - growing broilers (such as white - feather broilers) have a significantly higher incidence rate than local breeds due to their fast growth rate and weak stress - resistance ability.

[0022] (3)Impact of co - infection: When co - infected with Newcastle disease virus, infectious bronchitis virus, etc., the incidence rate and the severity of the disease increase significantly.

[0023] 6. Comprehensive prevention and control measures: (1)Purification of breeding stock and biosecurity: Purification of breeding hen flocks: Regular detection (randomly sampling 5% of sera / throat swabs per batch), culling positive breeding hens; before hatching, disinfect the breeding eggs by medicated bath with tylosin (500 ppm) for 30 minutes.

[0024] All - in - all - out management: Thoroughly disinfect the empty houses during the empty - house period (alternately using 2% caustic soda and peracetic acid) to block cross - infection between batches.

[0025] (2)Vaccination strategies: Live attenuated vaccines: Australian MS - H strain live vaccine (spray immunization, first immunization at 7 days of age, protection rate 80% - 90%), avoid using it simultaneously with NDV live vaccine.

[0026] Inactivated vaccines: MS inactivated vaccines developed by some domestic enterprises (such as Repbio) (immunized twice before laying, antibody persists for 6 months).

[0027] (3)Precise medication regimens, sensitive drugs: Tylosin (drinking water: 50 mg / L, used for 5 consecutive days); Doxycycline (mixing in feed: 20 mg / kg, used for 7 consecutive days); Tilmicosin (drinking water: 75 mg / L, used for 3 consecutive days, long withdrawal period).

[0028] [[ID=http: / / www.xxx.com]]Drug - resistance management: Conduct drug sensitivity tests every quarter, avoid continuous use of the same class of drugs (such as alternating macrolides and tetracyclines).

[0029] (4)Optimization of feeding management: Environmental control: Ammonia concentration < 10 ppm, humidity 60% - 65%, density ≤ 8 birds / ㎡ (breeding meat chickens).

[0030] Nutritional support: Add vitamin A (10,000 IU / kg), vitamin E (50 mg / kg), and organic selenium (0.3 ppm) to promote joint repair Latest research progress (2023 - 2024) (1) Development of new vaccines: Genetic engineering subunit vaccines: The team from the Chinese Academy of Agricultural Sciences developed a recombinant protein vaccine based on the OppD protein (adhesion factor) of MS. Tests showed that the challenge protection rate reached 85%.

[0031] Nucleic acid vaccines: The DNA vaccine (targeting the p80 gene of MS) developed by American scholars can induce mucosal immunity and reduce respiratory tract colonization.

[0032] (2) Drug resistance mechanisms and alternative therapies: Discovery of drug resistance genes: The resistance of MS to macrolides is related to the mutation of 23S rRNA gene (A2058G locus). Combined drug use (such as tylosin + lincomycin) is recommended.

[0033] Phage therapy: Israeli research screened out MS-specific phages (such as Myo1), and in vitro tests can reduce the pathogen load by 90%.

[0034] (3) Innovation in diagnostic techniques: CRISPR rapid detection: The fluorescence test strip based on CRISPR-Cas12a can detect MS within 15 minutes (sensitivity 102 CFU / mL), which is suitable for on-site screening.

[0035] Metabolomics analysis: Distinguish MS infection from viral arthritis through joint fluid metabolic markers (such as lactic acid, creatinine).

[0036] The prevention and control of Mycoplasma synoviae leg disease should take "purification as the basis, immunization as the supplement, precise medication, disease-resistant nutrition and biosecurity" as the core: (1) Breeder farms: Prioritize the implementation of the MS purification plan, and combine PCR detection to eliminate carrier chickens; (2) Commercial farms: Use live attenuated vaccines for immunization during the brooding period, and cooperate with environmental disinfection to reduce the risk of horizontal transmission; (3) Future directions: Accelerate the listing of domestic genetic engineering vaccines, promote the CRISPR rapid detection technology, and establish a regional MS drug resistance database.

[0037] (II) Technical progress in the prevention and control of Mycoplasma synoviae leg disease by functional additive compositions: 1. Background of Mycoplasma synoviae leg disease: Mycoplasma synoviae (MS) infection is a common disease in meat breeder chickens, mainly causing synovitis, lameness, and reduced growth performance. In severe cases, it affects the quality of breeding eggs and hatching rate. Traditional prevention and control rely on antibiotics, but the problem of drug resistance is prominent. Therefore, functional feed additive combinations have become a research hotspot.

[0038] 2. Prevention and control mechanisms of functional additive combinations: (1)Immune regulation: Enhance innate immunity and mucosal barrier function.

[0039] (2)Anti-inflammatory and antioxidant: Inhibit inflammatory factors (such as IL-6, TNF-α) and oxidative stress.

[0040] (3)Pathogen inhibition: Directly inhibit the adhesion and proliferation of MS.

[0041] (4)Improve intestinal health: Indirectly enhance disease resistance by regulating the intestinal flora.

[0042] 3. As shown in Table 1, the core additive combination and its functions: Table 1 Core additive combination and its functions

[0043] 4. Latest technology research: (1)Phage therapy: Phages specific to MS (such as those in the Myoviridae family) have entered the experimental stage, which can precisely eliminate pathogens and reduce the impact on the host flora.

[0044] (2)Yeast cell wall polysaccharides: The combined use of β-glucan and mannan oligosaccharide enhances the phagocytic ability of macrophages by activating the TLR pathway and reduces the MS load in joint synovial fluid.

[0045] (3)Assisted by gene editing technology: CRISPR-based gene editing has screened out candidate genes resistant to MS infection (such as MHC-II molecules), and combined with nutritional regulation to enhance genetic resistance.

[0046] (4)Nano-encapsulation technology: Curcumin nano-liposomes or oregano oil micro-encapsulation technology to improve bioavailability and extend the action time. Summary of the Invention

[0047] The technical solution of the present invention to solve the above technical problems is to provide a functional additive for preventing and treating synovitis mycoplasma leg disease in meat breeding chickens, which is composed of the following components in weight ratio: Immune active peptide from cicada slough 4%-8%; Curcumin 4%-8%; Bacillus subtilis 2%-4%; Xylanase complex 2%-6%; Chelated organic trace elements 14%-16%; 25-hydroxyvitamin D3 (0.05% content) 1.5%-2%; Sodium butyrate 10%-16%; Carrier The balance to 100%.

[0048] Furthermore, the chelated organic trace elements include manganese, zinc and selenium.

[0049] Furthermore, the carrier is puffed corn flour.

[0050] Furthermore, the viable cell count of the Bacillus subtilis is ≥ 2×10 11 CFU / g.

[0051] In order to solve the above technical problems, the present invention also provides a feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders, comprising the functional additive composition as described above, which is composed of the following components in the following weight ratios: Corn 60%-68%; Soybean meal 16%-28%; Stone powder 1.5%-6.5%; Calcium hydrogen phosphate 1.4%-1.9%; Lysine sulfate 0.2%-0.3%; Methionine 0.2%; Threonine 0.1%; Sodium bicarbonate 0.2%-0.25%; Choline chloride 0.08%-0.15%; Sodium chloride 0.25%; Premix 0.9%-1%; Functional additive composition 0.4%-0.6%.

[0052] Furthermore, the feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders further comprises: 4% by weight of puffed soybean meal (33% crude protein).

[0053] Furthermore, the feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders further comprises: 35 kg of wheat bran with a weight component of 3.5% and rice bran meal with a weight component of 3.5%.

[0054] Furthermore, the feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders further comprises: 1%-1.5% soybean oil by weight.

[0055] Furthermore, the feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders further comprises: sodium humate in an amount of 0.1%-0.2% by weight.

[0056] Furthermore, the feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders further comprises: 1% by weight of bentonite.

[0057] The functional additive designed by the present invention for preventing and treating Mycoplasma synoviae leg disease in broiler breeders can be added to feed to effectively prevent and treat MS infection rate and reduce it to below 5% (control group ≥5%). DETAILED DESCRIPTION

[0058] The present invention provides a functional additive and feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders, and aims to design a functional additive and feed for preventing and treating Mycoplasma synoviae leg disease in broiler breeders.

[0059] The functional additive and feed for preventing and treating synoviae leg disease in broiler breeders proposed by the present invention will be described in the following specific examples: A functional additive for preventing and treating Mycoplasma synoviae leg disease in broiler breeders, comprising the following components in the following weight ratios: Cicada slough immune active peptide 4%-8%; Curcumin 4%-8%; Bacillus subtilis 2%-4%; Xylanase 2%-6%; Chelated organic trace elements 14%-16%; 25-hydroxyvitamin D3 (0.05% content) 1.5%-2%; Sodium butyrate 10%-16%; The carrier balance was adjusted to 100%.

[0060] Furthermore, the chelated organic trace elements include manganese, zinc, and selenium, wherein the manganese is 60,000-90,000 mg / kg, the zinc is 60,000-90,000 mg / kg, and the selenium is 200-600 mg / kg.

[0061] Furthermore, the carrier is puffed corn flour.

[0062] Furthermore, the viable cell count of the Bacillus subtilis is ≥ 2×10 11 CFU / g. Example 1: A functional additive for preventing and treating Mycoplasma synoviae leg disease in broiler breeders, comprising the following components in the following weight ratios:

[0063] Cicada slough immune active peptide 4%; Curcumin 4%; Bacillus subtilis 2%; Xylanase 2%; Chelated organic trace elements 14%; 25-hydroxyvitamin D3 (0.05% content) 1.5%; Sodium butyrate 10%; Carrier 62.5%.

[0064] Further, the chelated organic trace elements include manganese, zinc, and selenium, wherein manganese is 60,000 - 90,000 mg / kg, zinc is 60,000 - 90,000 mg / kg, and selenium is 200 - 600 mg / kg.

[0065] Further, the carrier is expanded corn flour.

[0066] Further, the viable count of the Bacillus subtilis ≥ 2×10 11 CFU / g. Example 2: A functional additive for preventing and treating synovitis mycoplasma leg disease in meat breeding chickens, which is composed of the following components in weight ratio:

[0067] Immune active peptide of cicada slough 8%; Curcumin 8%; Bacillus subtilis 4%; Xylan complex enzyme 6%; Chelated organic trace elements 16%; 25-hydroxyvitamin D3 (0.05% content) 2%; Sodium butyrate 16%; Carrier 40%.

[0068] Further, the chelated organic trace elements include manganese, zinc, and selenium, wherein manganese is 60,000 - 90,000 mg / kg, zinc is 60,000 - 90,000 mg / kg, and selenium is 200 - 600 mg / kg.

[0069] Further, the carrier is expanded corn flour.

[0070] Further, the viable count of the Bacillus subtilis ≥ 2×10 11 CFU / g. Example 3: A feed for preventing and treating synovitis mycoplasma leg disease in meat breeding chickens, which is composed of the following components in weight ratio:

[0071] Corn 61.02%; Soybean meal 28%; Limestone powder (100 mesh) 1.5%; Calcium hydrogen phosphate 1.85%; L-lysine sulfate (70%) 0.25%; DL-methionine (99%) 0.2%; L-threonine 0.1%; Sodium bicarbonate 0.25%; Choline chloride (60%) 0.08%; Sodium chloride 0.25%; Premix 0.9%; Functional additive composition: 0.5%; Extruded soybean meal (crude protein 33%): 4%; Sodium humate: 0.1%; Bentonite: 1%.

[0072] For 1000 kg of feed: The metabolic energy of the feed is 2800 Kcal / Kg; crude protein ≥ 19.0%; crude fiber ≤ 6.0%; crude ash ≤ 9.0%; calcium 0.50 - 2.50%; total phosphorus ≥ 0.40%; sodium chloride 0.1 - 1.0%; methionine ≥ 0.35 - 0.90%; moisture ≤ 14.0%.

[0073] Among them, the premix includes 350 g of compound vitamins for breeding hens (purchased from Jilin Xinfangyuan Animal Husbandry Technology Co., Ltd., production license number: Jisi Zheng (2018) 01023); 400 g of phytase (10000 U / g); 300 g of compound enzymes for broiler chickens; 200 g of Bacillus subtilis (1.0×106 CFU / g); 400 g of tannic acid (≥50%); 1000 g of compound mold inhibitor (propionate salts); 500 g of betaine (30%); 150 g of antioxidant (ethoxyquinoline); 5700 g of zeolite; total 9 kg. Example 4: A feed for preventing and treating synovial bursa mycoplasma leg disease in breeding hens, which is composed of the following components in weight ratio:

[0074] Corn: 66.95%; Soybean meal: 19%; Limestone powder (40 mesh): 1.7%; Calcium hydrogen phosphate: 1.6%; L-lysine sulfate (70%): 0.3%; DL-methionine (99%): 0.2%; Threonine: 0.1%; Sodium bicarbonate: 0.2%; Choline chloride (60%): 0.1%; Sodium chloride: 0.25%; Premix: 0.9%; Functional additive composition: 0.5%; Wheat bran: 3.5% Rice bran meal: 3.5% Soybean oil: 0.1% Sodium humate: 0.2%.

[0075] 1000 kg of feed: The metabolizable energy of the feed is 2700 Kcal / Kg; crude protein ≥ 15.0%; crude fiber ≤ 7.0%; crude ash ≤ 10.0%; calcium 0.50 - 2.50%; total phosphorus ≥ 0.40%; sodium chloride 0.1 - 1.0%; methionine ≥ 0.35 - 0.90%; moisture ≤ 14.0%.

[0076] Among them, the premix includes 350 g of compound vitamins for breeding hens (purchased from Jilin Xinfangyuan Animal Husbandry Technology Co., Ltd., production license number: Jisi Zheng (2018) 01023); 400 g of phytase (10000 U / g); 200 g of compound enzymes for broiler chickens; 200 g of Bacillus subtilis (1.0×10 6 CFU / g); 300 g of tannic acid (≥50%); 1000 g of compound mold inhibitor (propionate); 450 g of betaine (30%); 150 g of antioxidant (ethoxyquinoline); 5950 g of zeolite; with a total of 9 kg. Example 5: A feed for preventing and treating synovitis mycoplasma leg disease in breeding hens, which is composed of the following components in weight ratio:

[0077] Corn 66.3%; Soybean meal 16.5%; Limestone powder 6.5% (15 mesh) + 1.5% (30 mesh); Calcium hydrogen phosphate 1.4%; L-lysine sulfate (70%) 0.3%; Methionine (99%) 0.2%; Threonine 0.1%; Sodium bicarbonate 0.2%; Choline chloride (60%) 0.15%; Sodium chloride 0.25%; Premix 0.9%; Functional additive composition 0.5%; Extruded soybean powder (crude protein 33%) 4%; Soybean oil 0.15%.

[0078] 1000 kg of feed: The metabolizable energy of the feed is 2750 Kcal / Kg; crude protein ≥ 16.0%; crude fiber ≤ 8.0%; crude ash ≤ 15.0%; calcium 2 - 6%; total phosphorus ≥ 0.50%; sodium chloride 0.1 - 1.0%; methionine ≥ 0.35 - 0.90%; moisture ≤ 14.0%.

[0079] Among them, the premix includes 400 g of compound vitamins for breeding chickens (purchased from Jilin Xinfangyuan Animal Husbandry Technology Co., Ltd., production license number: Jisi Zheng (2018) 01023); 400 g of phytase (10,000 U / g); 300 g of compound enzymes for broiler chickens; 200 g of Bacillus subtilis (1.0×10 6 CFU / g); 300 g of tannic acid (≥50%); 1000 g of compound mold inhibitor (propionate); 500 g of betaine (30%); 150 g of antioxidant (ethoxyquinoline); 6750 g of zeolite; with a total of 9 kg.

[0080] Rearing experiment of meat breeding chickens: 1. Experimental grouping: Select meat breeding chickens (AA+) at 25 weeks of age in 4 chicken houses that have entered the laying period until they are culled (56 - 57 weeks). There are 6000 - 7000 breeding chickens in each chicken house, and each chicken house is set as a group. The meat breeding chickens are divided into 4 groups (conventional feed groups (A, B), experimental feed groups (C, D)). There are 6000 chickens in group A, 6200 chickens in group B, 6700 chickens in group C, and 6000 chickens in group D (conventional feed groups A and B are the feeds disclosed in Example 3, and the feeds do not include the functional additive composition; experimental feed groups C and D are the feeds disclosed in Example 3 and include the functional additive composition, and the functional additive composition is the functional additive composition disclosed in Example 1). The daily feeding management and prevention and control take "purification as the basis, immunization as the supplement, precise medication, disease-resistant nutrition, and biosecurity" as the core, and adopt the four-in-one strategy of "drug purification + vaccine immunization + environmental management + disease-resistant nutrition". Through the functional additive combination, multi-target synergistic effects can be achieved, and it can effectively prevent and control the synovitis mycoplasma leg disease of meat breeding chickens.

[0081] Experimental results: Under the conditions of standardized feeding management, by adding a functional additive composition for preventing and treating synovitis mycoplasma (MS) leg disease of meat breeding chickens to the breeding chicken feed, the incidence rate of synovitis mycoplasma leg disease during the laying period of meat breeding chickens can be significantly reduced (reduced to 0 - 2%), and the average egg production rate can be increased (increased by 8.5%). The incidence rate of the conventional feed groups (A, B) of meat breeding chickens is 6 - 7%, and the average egg production rate is 62 - 66%; the incidence rate of the experimental feed groups (C, D) is 0 - 2%, and the average egg production rate is 72 - 73%. The statistical results of the feeding experiment are shown in Table 2 (the data are from the daily management data and experimental data of the chicken farm).

[0082] Table 2 Feeding experiment results:

[0083] As described above, it is only the preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. A functional additive for preventing and treating Mycoplasma synoviae leg disease in meat breeding chickens, characterized in that, It consists of components with the following weight ratios: Immune-active peptides from cicada slough 4% - 8%; Curcumin 4% - 8%; Bacillus subtilis 2% - 4%; Xylanase complex 2% - 6%; Chelated organic trace elements 14% - 16%; 25-hydroxyvitamin D3 1.5% - 2%; Sodium butyrate 10% - 16%; Carrier, the balance to 100%.

2. A functional additive for preventing and treating synovitis mycoplasma leg disease in meat breeding chickens, characterized in that, The chelated organic trace elements include manganese, zinc, and selenium.

3. A functional additive for preventing and treating Mycoplasma synoviae leg disease in meat breeding chickens, characterized in that, The carrier is expanded corn flour.

4. A functional additive for preventing and treating Mycoplasma synoviae leg disease in meat breeding chickens, characterized in that, The viable count of the Bacillus subtilis ≥ 2×10 11 CFU / g.

5. A feed for preventing and treating synovitis mycoplasma leg disease in meat - type breeding chickens, characterized in that, Containing the functional additive composition described in any one of claims 1 to 4, it consists of components with the following weight ratios: Corn 60% - 68%; Soybean meal 16% - 28%; Limestone powder 1.5% - 6.5%; Calcium hydrogen phosphate 1.4% - 1.9%; L-lysine sulfate 0.2% - 0.3%; Methionine 0.2%; Threonine 0.1%; Sodium bicarbonate 0.2% - 0.25%; Choline chloride 0.08% - 0.15%; Sodium chloride 0.25%; Premix 0.9% - 1%; Functional additive composition 0.4% - 0.6%.

6. The feed for preventing and treating synovitis mycoplasma leg disease of meat - type breeding chickens according to claim 5, wherein, It also includes: Expanded soybean powder with a weight ratio of 4%.

7. The feed for preventing and treating Mycoplasma synoviae leg disease in meat - type breeding chickens according to claim 5, wherein, It also includes: Wheat bran with a weight ratio of 3.5% (35 kg) and rice bran meal with a weight ratio of 3.5%.

8. The feed for preventing and treating Mycoplasma synoviae leg disease in meat - type breeding chickens according to claim 5, wherein, It also includes: Soybean oil with a weight ratio of 1% - 1.5%.

9. The feed for preventing and treating synovitis mycoplasma leg disease of meat breeding chickens according to claim 5, wherein, It also includes: Sodium humate with a weight ratio of 0.1% - 0.2%.

10. The feed for preventing and treating Mycoplasma synoviae leg disease in meat - type breeding chickens according to claim 5, wherein, It also includes: Bentonite with a weight ratio of 1%.

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