Preparation process of blue-green algae organic fertilizer

Abstract

The preparation process comprises the following steps: S100, mixing humic acid, monoammonium phosphate and potassium sulfate to obtain a first mixture; s200, mixing the first mixture with the blue-green algae extract to obtain a second mixture; s300, mixing the second mixture, sodium alginate, chelated zinc, chelated iron and chelated magnesium to obtain a third mixture; s400, mixing peroxidized humic acid, magnesium sulfate and monopotassium phosphate to obtain a fourth mixture; and S500, adding the fourth mixture into the third mixture to obtain the blue-green algae organic fertilizer. The cyanobacteria water-soluble fertilizer prepared by the process is good in absorption, obvious in fertilization effect and small in fertilization amount, and manpower and time used for fertilization can be reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of organic fertilizer preparation, and more particularly, to a preparation process of cyanobacteria organic fertilizer. Background Art

[0002] Cyanobacteria are algal organisms, also known as blue-green algae. Among all algal organisms, cyanobacteria are the simplest and most primitive ones. Approximately 3.5 - 3.3 billion years ago, cyanobacteria already appeared on the earth. Now about 2,000 species of cyanobacteria are known, and about 900 species have been recorded in China. They are widely distributed all over the world, but most are freshwater products and a few are marine products.

[0003] Cyanobacteria often multiply in large numbers from June to September in summer and form a layer of blue-green and foul-smelling floating foam on the water surface, which is called "water bloom". A large-scale cyanobacteria outbreak is called "green tide". The massive reproduction of cyanobacteria leads to a decrease in the oxygen content and a lack of light in the water, thus deteriorating the living environment of fish in the water and causing the death of filamentous algae and planktonic algae in the water because they cannot synthesize the nutrients they need. Due to hypoxia or even anoxia and the deterioration of water quality, microcystis in cyanobacteria will also produce toxins. These toxins not only directly poison fish, humans and livestock, but are also strong carcinogens, and these toxins are heat-resistant and not easily decomposed by boiling water.

[0004] Since cyanobacteria are easy to grow and can easily have an adverse impact on water quality, how to utilize cyanobacteria is a problem that needs to be solved. In the prior art, cyanobacteria have been used to prepare organic fertilizers, but the cyanobacteria organic fertilizers prepared by the current methods have poor absorption, require a large amount of fertilization, and are time-consuming and laborious. Therefore, there is an urgent need to prepare a cyanobacteria organic fertilizer with good absorption, less fertilization amount, and time-saving and labor-saving. Summary of the Invention

[0005] The present invention provides a preparation process of cyanobacteria organic fertilizer. The cyanobacteria water-soluble fertilizer is prepared by this process. The organic fertilizer has good absorption, obvious fertilization effect, and less fertilization amount, and can reduce the labor and time used for fertilization.

[0006] The technical solution adopted by the present invention to solve the above technical problems is: a preparation process of cyanobacteria organic fertilizer, comprising: S100: Mix humic acid, monoammonium phosphate, and potassium sulfate to obtain a first mixture; S200: Mix the first mixture and cyanobacteria extract to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate to obtain a fourth mixture; S500: Add the fourth mixture to the third mixture to obtain cyanobacteria organic fertilizer.

[0007] Further, S100 specifically includes the following steps: Add humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of (8 - 12):(4 - 8):(5 - 10) to a stirrer, and then stir at a speed of 180 r / min - 240 r / min for 20 min - 40 min to obtain the first mixture.

[0008] Further, S200 specifically includes the following steps: Add the first mixture and cyanobacteria extract with a mass ratio of (15 - 30):(15 - 20) to a stirrer, and then stir at a speed of 200 r / min - 300 r / min for 40 min - 80 min to obtain the second mixture.

[0009] Further, S300 specifically includes the following steps: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of (30 - 50):(1 - 2):(0.6 - 0.8):(0.2 - 0.4):(0.7 - 0.9), and then stir at a speed of 100 r / min - 150 r / min for 15 min - 25 min to obtain the third mixture.

[0010] Further, the cyanobacteria extract is prepared by the following method: S610: Dry and grind cyanobacteria to obtain cyanobacteria powder; S620: Take the cyanobacteria powder for one - time water extraction to obtain the first filtrate and the first filter residue; S630: Perform secondary water extraction on the first filtrate to obtain the second filtrate and the second filter residue; S640: Add the first filter residue and the second filter residue to water, and then add cellulase to it. After enzymatic hydrolysis, obtain cyanobacteria enzymatic hydrolysate; S650: Add magnesium sulfate and sodium chloride to the cyanobacteria enzymatic hydrolysate, and let it stand for 5 - 10 days to obtain the fermentation material; S660: Mix the fermentation material and the second filtrate to obtain the cyanobacteria extract.

[0011] Further, in S620, mix cyanobacteria powder, citric acid, and water with a mass ratio of 1:(2 - 3):(15 - 20), and then perform water extraction at a temperature of 60°C - 70°C for 2 h - 4 h. After filtration, obtain the first filtrate and the second filtrate.

[0012] Further, in S630, the primary filtrate and sodium hydroxide with a mass ratio of (1 - 1.5):(0.02 - 0.1) are mixed, and then water extraction is carried out at a temperature of 60°C - 70°C for 1 h - 2 h. After filtration, a secondary filtrate and secondary filter residue are obtained.

[0013] Further, in S640, the primary filter residue, secondary filter residue, cellulase, and water with a mass ratio of (1 - 1.1):(0.01 - 0.03):(0.02 - 0.05):(5 - 8) are mixed, and then heated to 50°C - 60°C for enzymatic hydrolysis for 4 h - 5 h to obtain a cyanobacteria enzymatic hydrolysate.

[0014] Further, S400 specifically includes the following steps: The peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of (1 - 2):(5 - 10):(4 - 8) are mixed, and then left to stand for 40 min - 60 min to obtain a fourth mixture.

[0015] Further, S500 specifically includes the following steps: The third mixture and the fourth mixture with a mass ratio of (30 - 55):(1 - 2) are mixed, and then stirred at a speed of 60 r / min - 100 r / min for 5 min - 15 min to obtain a cyanobacteria organic fertilizer.

[0016] Compared with the prior art, the advantages of the present invention are as follows: The cyanobacteria organic fertilizer prepared by the method of the present invention is water-soluble. Compared with the existing cyanobacteria organic fertilizers, this organic fertilizer has good absorption, obvious fertilization effect, and less fertilization amount, which can reduce the labor and time used for fertilization. Moreover, the cyanobacteria extract used in the present invention not only contains rich biological polysaccharides but also contains a certain amount of photosynthetic bacteria, which can maintain the fertility of the soil and promote the growth of crops. Specific Embodiments

[0017] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following detailed description of the specific embodiments of the present invention is provided.

[0018] The present invention provides a preparation process for a cyanobacteria organic fertilizer, including: S100: Mix humic acid, monoammonium phosphate, and potassium sulfate to obtain a first mixture; S200: Mix the first mixture and a cyanobacteria extract to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate to obtain a fourth mixture; S500: Add the fourth mixture to the third mixture to obtain the cyanobacteria organic fertilizer.

[0019] Among them, the cyanobacteria extract contains biological polysaccharides and photosynthetic bacteria. For the convenience of understanding, the present invention also provides a preparation method of the cyanobacteria extract: S610: Dry and grind the cyanobacteria to obtain cyanobacteria powder; S620: Take the cyanobacteria powder for the first water extraction to obtain the first filtrate and the first filter residue; S630: Perform a second water extraction on the first filtrate to obtain the second filtrate and the second filter residue; S640: Add the first filter residue and the second filter residue to water, and then add cellulase thereto. After enzymatic hydrolysis, obtain the cyanobacteria enzymatic hydrolysate; S650: Add magnesium sulfate and sodium chloride to the cyanobacteria enzymatic hydrolysate, and let it stand for 5 - 10 days to obtain the fermentation material; S660: Mix the fermentation material and the second filtrate to obtain the cyanobacteria extract.

[0020] Specifically, in S610, air-dry the cyanobacteria at a temperature of 60 °C, and then put it into a grinder to grind it into cyanobacteria powder with a particle size of 0.1 mm - 0.5 mm.

[0021] Specifically, in S620, mix the cyanobacteria powder, citric acid, and water with a mass ratio of 1:(2 - 3):(15 - 20), and then perform water extraction at a temperature of 60 °C - 70 °C for 2 h - 4 h. After filtration, obtain the first filtrate and the second filtrate. Preferably, mix the cyanobacteria powder, citric acid, and water with a mass ratio of 1:2.3:18, and then perform water extraction at a temperature of 65 °C for 3 h.

[0022] Specifically, in S630, mix the first filtrate and sodium hydroxide with a mass ratio of (1 - 1.5):(0.02 - 0.1), and then perform water extraction at a temperature of 60 °C - 70 °C for 1 h - 2 h. After filtration, obtain the second filtrate and the second filter residue. Preferably, mix the first filtrate and sodium hydroxide with a mass ratio of 1.2:0.05, and then perform water extraction at a temperature of 65 °C for 1.5 h.

[0023] Specifically, in S640, mix the first filter residue, the second filter residue, cellulase, and water with a mass ratio of (1 - 1.1):(0.01 - 0.03):(0.02 - 0.05):(5 - 8), and then heat it to 50 °C - 60 °C and perform enzymatic hydrolysis for 4 h - 5 h to obtain the cyanobacteria enzymatic hydrolysate. Preferably, mix the first filter residue, the second filter residue, cellulase, and water with a mass ratio of 1.03:0.02:0.03:7, and then heat it to 55 °C and perform enzymatic hydrolysis for 4.5 h.

[0024] Specifically, in S650, the blue-green algae enzymatic hydrolysate, magnesium sulfate, and sodium chloride with a mass ratio of 8:0.07:0.1 are mixed, and then left standing for 7 days to obtain the fermentation material.

[0025] Specifically, in S660, the fermentation material and the secondary filtrate with a mass ratio of 1:1 are mixed to obtain the blue-green algae extract.

[0026] For the convenience of operation and understanding, the preparation process of the blue-green algae organic fertilizer is described in detail below.

[0027] Among them, S100 specifically includes the following steps: Humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of (8 - 12):(4 - 8):(5 - 10) are added to a stirrer, and then stirred at a speed of 180 r / min - 240 r / min for 20 min - 40 min to obtain the first mixture.

[0028] Preferably, humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of 10:6:8 are added to the stirrer.

[0029] Preferably, it is stirred at a speed of 200 r / min for 30 min to obtain the first mixture.

[0030] Among them, S200 specifically includes the following steps: The first mixture and the blue-green algae extract with a mass ratio of (15 - 30):(15 - 20) are added to a stirrer, and then stirred at a speed of 200 r / min - 300 r / min for 40 min - 80 min to obtain the second mixture.

[0031] Preferably, the first mixture and the blue-green algae extract with a mass ratio of 24:17 are added to the stirrer.

[0032] Preferably, it is stirred at a speed of 260 r / min for 60 min to obtain the second mixture.

[0033] Among them, S300 specifically includes the following steps: The second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of (30 - 50):(1 - 2):(0.6 - 0.8):(0.2 - 0.4):(0.7 - 0.9) are mixed, and then stirred at a speed of 100 r / min - 150 r / min for 15 min - 25 min to obtain the third mixture.

[0034] Preferably, the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of 41:1.3:0.7:0.3:0.8 are mixed.

[0035] Preferably, it is stirred at a speed of 120 r / min for 20 min to obtain the third mixture.

[0036] Among them, S400 specifically includes the following steps: Mix humic acid peroxide, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of (1 - 2):(5 - 10):(4 - 8), and then let it stand for 40 min - 60 min to obtain a fourth mixture.

[0037] Preferably, mix humic acid peroxide, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of 1.5:8:6.

[0038] Preferably, let it stand for 50 min to obtain a fourth mixture.

[0039] Among them, S500 specifically includes the following steps: Mix the third mixture and the fourth mixture with a mass ratio of (30 - 55):(1 - 2), and then stir at a speed of 60 r / min - 100 r / min for 5 min - 15 min to obtain cyanobacteria organic fertilizer.

[0040] Preferably, mix the third mixture and the fourth mixture with a mass ratio of 44:1.8.

[0041] Preferably, stir at a speed of 80 r / min for 10 min to obtain cyanobacteria organic fertilizer. Example

[0042] This example provides a preparation process of cyanobacteria organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of 10:6:8 to a stirrer, and stir at a speed of 200 r / min for 30 min to obtain a first mixture; S200: Add the first mixture and cyanobacteria extract with a mass ratio of 24:17 to a stirrer, and stir at a speed of 260 r / min for 60 min to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain a third mixture; S400: Mix humic acid peroxide, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of 1.5:8:6, and let it stand for 50 min to obtain a fourth mixture; S500: Mix the third mixture and the fourth mixture with a mass ratio of 44:1.8, and stir at a speed of 80 r / min for 10 min to obtain cyanobacteria organic fertilizer.

[0043] Among them, the cyanobacteria extract is prepared by the following method: S610: Air-dry cyanobacteria at a temperature of 60 °C, and then put it into a grinder to grind it into cyanobacteria powder; S620: Mix the blue-green algae powder, citric acid, and water in a mass ratio of 1:2.3:18, then perform water extraction at 65°C for 3 hours, and filter to obtain a first filtrate and a second filtrate; S630: Mix the first filtrate and sodium hydroxide in a mass ratio of 1.2:0.05, then perform water extraction at 65°C for 1.5 hours, and filter to obtain a second filtrate and second filter residue; S640: Mix the first filter residue, second filter residue, cellulase, and water in a mass ratio of 1.03:0.02:0.03:7, then heat to 55°C and perform enzymatic hydrolysis for 4.5 hours to obtain a blue-green algae enzymatic hydrolysate; S650: Mix the blue-green algae enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, then let it stand for 7 days to obtain a fermentation material; S660: Mix the fermentation material and the second filtrate in a mass ratio of 1:1 to obtain a blue-green algae extract. Example

[0044] This example provides a preparation process for a blue-green algae organic fertilizer, including: S100: Add humic acid and potassium sulfate in a mass ratio of 10:14 to a stirrer, stir at a speed of 200 r / min for 30 minutes to obtain a first mixture; S200: Add the first mixture and the blue-green algae extract in a mass ratio of 24:17 to a stirrer, stir at a speed of 260 r / min for 60 minutes to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium in a mass ratio of 41:1.3:0.7:0.3:0.8, stir at a speed of 120 r / min for 20 minutes to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1.5:8:6, let it stand for 50 minutes to obtain a fourth mixture; S500: Mix the third mixture and the fourth mixture in a mass ratio of 44:1.8, stir at a speed of 80 r / min for 10 minutes to obtain a blue-green algae organic fertilizer.

[0045] Among them, the blue-green algae extract is prepared by the following method: S610: Air-dry the blue-green algae at 60°C, then put it into a grinder and grind it into blue-green algae powder; S620: Mix the blue-green algae powder, citric acid, and water in a mass ratio of 1:2.3:18, then perform water extraction at 65°C for 3 hours, and filter to obtain a first filtrate and a second filtrate; S630: Mix the first filtrate and sodium hydroxide in a mass ratio of 1.2:0.05, then perform water extraction at 65°C for 1.5 h, and obtain a second filtrate and second filter residue after filtration; S640: Mix the first filter residue, second filter residue, cellulase, and water in a mass ratio of 1.03:0.02:0.03:7, then heat to 55°C and perform enzymatic hydrolysis for 4.5 h to obtain a blue-green algae enzymatic hydrolysate; S650: Mix the blue-green algae enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, then let it stand for 7 days to obtain a fermentation material; S660: Mix the fermentation material and the second filtrate in a mass ratio of 1:1 to obtain a blue-green algae extract. Example

[0046] This example provides a preparation process for a blue-green algae organic fertilizer, including: S100: Add humic acid and monoammonium phosphate in a mass ratio of 10:14 to a stirrer, and stir at a speed of 200 r / min for 30 min to obtain a first mixture; S200: Add the first mixture and the blue-green algae extract in a mass ratio of 24:17 to a stirrer, and stir at a speed of 260 r / min for 60 min to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium in a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1.5:8:6, and let it stand for 50 min to obtain a fourth mixture; S500: Mix the third mixture and the fourth mixture in a mass ratio of 44:1.8, and stir at a speed of 80 r / min for 10 min to obtain a blue-green algae organic fertilizer.

[0047] Among them, the blue-green algae extract is prepared by the following method: S610: Air-dry blue-green algae at 60°C, and then put it into a grinder to grind it into blue-green algae powder; S620: Mix the blue-green algae powder, citric acid, and water in a mass ratio of 1:2.3:18, then perform water extraction at 65°C for 3 h, and obtain a first filtrate and a second filtrate after filtration; S630: Mix the first filtrate and sodium hydroxide in a mass ratio of 1.2:0.05, then perform water extraction at 65°C for 1.5 h, and obtain a second filtrate and second filter residue after filtration; S640: Mix the primary filter residue, secondary filter residue, cellulase, and water in a mass ratio of 1.03:0.02:0.03:7, then heat to 55 °C and enzymatically hydrolyze for 4.5 h to obtain a blue-green algae enzymatic hydrolysate; S650: Mix the blue-green algae enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, then let it stand for 7 days to obtain a fermentation material; S660: Mix the fermentation material and the secondary filtrate in a mass ratio of 1:1 to obtain a blue-green algae extract. Example

[0048] This example provides a preparation process for a blue-green algae organic fertilizer, including: S100: Add monoammonium phosphate and potassium sulfate in a mass ratio of 12:16 to a stirrer and stir at a speed of 200 r / min for 30 min to obtain a first mixture; S200: Add the first mixture and the blue-green algae extract in a mass ratio of 24:17 to a stirrer and stir at a speed of 260 r / min for 60 min to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium in a mass ratio of 41:1.3:0.7:0.3:0.8 and stir at a speed of 120 r / min for 20 min to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1.5:8:6, let it stand for 50 min to obtain a fourth mixture; S500: Mix the third mixture and the fourth mixture in a mass ratio of 44:1.8 and stir at a speed of 80 r / min for 10 min to obtain a blue-green algae organic fertilizer.

[0049] Among them, the blue-green algae extract is prepared by the following method: S610: Air-dry blue-green algae at a temperature of 60 °C, then put it into a grinder and grind it into blue-green algae powder; S620: Mix the blue-green algae powder, citric acid, and water in a mass ratio of 1:2.3:18, then perform water extraction at a temperature of 65 °C for 3 h, and filter to obtain a first filtrate and a second filtrate; S630: Mix the first filtrate and sodium hydroxide in a mass ratio of 1.2:0.05, then perform water extraction at a temperature of 65 °C for 1.5 h, and filter to obtain a second filtrate and a secondary filter residue; S640: Mix the primary filter residue, secondary filter residue, cellulase, and water in a mass ratio of 1.03:0.02:0.03:7, then heat to 55 °C and enzymatically hydrolyze for 4.5 h to obtain a blue-green algae enzymatic hydrolysate; S650: Mix the cyanobacteria enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, and then let it stand for 7 days to obtain the fermentation material. S660: Mix the fermentation material and the secondary filtrate in a mass ratio of 1:1 to obtain the cyanobacteria extract. Example

[0050] This example provides a preparation process for cyanobacteria organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate in a mass ratio of 10:6:8 to a stirrer, and stir at a speed of 200 r / min for 30 min to obtain the first mixture. S200: Add the first mixture and cyanobacteria powder in a mass ratio of 24:17 to a stirrer, and stir at a speed of 260 r / min for 60 min to obtain the second mixture. S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium in a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain the third mixture. S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1.5:8:6, and let it stand for 50 min to obtain the fourth mixture. S500: Mix the third mixture and the fourth mixture in a mass ratio of 44:1.8, and stir at a speed of 80 r / min for 10 min to obtain the cyanobacteria organic fertilizer.

[0051] Among them, the cyanobacteria powder is prepared by the following method: Air-dry the cyanobacteria at a temperature of 60 °C, and then put it into a grinder to grind it into cyanobacteria powder. Example

[0052] This example provides a preparation process for cyanobacteria organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate in a mass ratio of 10:6:8 to a stirrer, and stir at a speed of 200 r / min for 30 min to obtain the first mixture. S200: Add the first mixture and cyanobacteria extract in a mass ratio of 24:17 to a stirrer, and stir at a speed of 260 r / min for 60 min to obtain the second mixture. S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium in a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain the third mixture. S400: Mix humic acid peroxide, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of 1.5:8:6, and let it stand for 50 min to obtain the fourth mixture. S500: Mix the third mixture and the fourth mixture with a mass ratio of 44:1.8, and stir at a speed of 80 r / min for 10 min to obtain the blue-green algae organic fertilizer.

[0053] Among them, the blue-green algae extract is prepared by the following method: S610: Air-dry the blue-green algae at a temperature of 60 °C, and then put it into a grinder to grind it into blue-green algae powder. S620: Mix the blue-green algae powder, citric acid, and water with a mass ratio of 1:2.3:18, and then perform water extraction at a temperature of 65 °C for 3 h. After filtration, obtain the first filtrate and the second filtrate. S630: Mix the first filtrate and sodium hydroxide with a mass ratio of 1.2:0.05, and then perform water extraction at a temperature of 65 °C for 1.5 h. After filtration, obtain the second filtrate and the second filter residue. Among them, the second filtrate is the blue-green algae extract. Example

[0054] This example provides a preparation process for blue-green algae organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of 1:6:8 to a stirrer, and stir at a speed of 200 r / min for 30 min to obtain the first mixture. S200: Add the first mixture and the blue-green algae extract with a mass ratio of 24:17 to a stirrer, and stir at a speed of 260 r / min for 60 min to obtain the second mixture. S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain the third mixture. S400: Mix humic acid peroxide, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of 1.5:8:6, and let it stand for 50 min to obtain the fourth mixture. S500: Mix the third mixture and the fourth mixture with a mass ratio of 44:1.8, and stir at a speed of 80 r / min for 10 min to obtain the blue-green algae organic fertilizer.

[0055] Among them, the blue-green algae extract is prepared by the following method: S610: Air-dry the blue-green algae at a temperature of 60 °C, and then put it into a grinder to grind it into blue-green algae powder. S620: Mix the cyanobacteria powder, cellulase, and water in a mass ratio of 1.05:0.03:7, then heat to 55 °C and enzymatically hydrolyze for 4.5 h to obtain a cyanobacteria enzymatic hydrolysate; S630: Mix the cyanobacteria enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, then let it stand for 7 days to obtain a cyanobacteria extract. Example

[0056] This example provides a preparation process for cyanobacteria organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate in a mass ratio of 10:6:8 to a stirrer and stir at a speed of 200 r / min for 30 min to obtain a first mixture; S200: Add the first mixture and the cyanobacteria extract in a mass ratio of 24:17 to a stirrer and stir at a speed of 260 r / min for 60 min to obtain a second mixture; S300: Mix the second mixture and sodium alginate in a mass ratio of 41:1.3 and stir at a speed of 120 r / min for 20 min to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1.5:8:6, let it stand for 50 min to obtain a fourth mixture; S500: Mix the third mixture and the fourth mixture in a mass ratio of 44:1.8 and stir at a speed of 80 r / min for 10 min to obtain cyanobacteria organic fertilizer.

[0057] Among them, the cyanobacteria extract is prepared by the following method: S610: Air-dry the cyanobacteria at a temperature of 60 °C, then put it into a grinder and grind it into cyanobacteria powder; S620: Mix the cyanobacteria powder, citric acid, and water in a mass ratio of 1:2.3:18, then perform water extraction at a temperature of 65 °C for 3 h, and filter to obtain a first filtrate and a second filtrate; S630: Mix the first filtrate and sodium hydroxide in a mass ratio of 1.2:0.05, then perform water extraction at a temperature of 65 °C for 1.5 h, and filter to obtain a second filtrate and a second filter residue; S640: Mix the first filter residue, the second filter residue, cellulase, and water in a mass ratio of 1.03:0.02:0.03:7, then heat to 55 °C and enzymatically hydrolyze for 4.5 h to obtain a cyanobacteria enzymatic hydrolysate; S650: Mix the cyanobacteria enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, then let it stand for 7 days to obtain a fermentation material; S660: Mix the fermented material and the secondary filtrate in a mass ratio of 1:1 to obtain a blue-green algae extract. Example

[0058] This example provides a preparation process for a blue-green algae organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate in a mass ratio of 10:6:8 to a stirrer, and stir at a speed of 200 r / min for 30 min to obtain a first mixture; S200: Add the first mixture and the blue-green algae extract in a mass ratio of 24:17 to a stirrer, and stir at a speed of 260 r / min for 60 min to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium in a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain a third mixture; S400: Mix humic acid, magnesium sulfate, and potassium dihydrogen phosphate in a mass ratio of 1.5:8:6, and let it stand for 50 min to obtain a fourth mixture; S500: Mix the third mixture and the fourth mixture in a mass ratio of 44:1.8, and stir at a speed of 80 r / min for 10 min to obtain the blue-green algae organic fertilizer.

[0059] Among them, the blue-green algae extract is prepared by the following method: S610: Air-dry the blue-green algae at a temperature of 60 °C, and then put it into a grinder to grind it into blue-green algae powder; S620: Mix the blue-green algae powder, citric acid, and water in a mass ratio of 1:2.3:18, and then perform water extraction at a temperature of 65 °C for 3 h. After filtration, obtain a first filtrate and a second filtrate; S630: Mix the first filtrate and sodium hydroxide in a mass ratio of 1.2:0.05, and then perform water extraction at a temperature of 65 °C for 1.5 h. After filtration, obtain a secondary filtrate and secondary filter residue; S640: Mix the first filter residue, secondary filter residue, cellulase, and water in a mass ratio of 1.03:0.02:0.03:7, and then heat to 55 °C and perform enzymatic hydrolysis for 4.5 h to obtain a blue-green algae enzymatic hydrolysate; S650: Mix the blue-green algae enzymatic hydrolysate, magnesium sulfate, and sodium chloride in a mass ratio of 8:0.07:0.1, and then let it stand for 7 days to obtain a fermented material; S660: Mix the fermented material and the secondary filtrate in a mass ratio of 1:1 to obtain a blue-green algae extract. Example

[0060] This embodiment provides a preparation process for cyanobacteria organic fertilizer, including: S100: Add humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of 10:6:8 into a stirrer, and stir at a speed of 200 r / min for 30 min to obtain a first mixture; S200: Add the first mixture and cyanobacteria extract with a mass ratio of 24:17 into a stirrer, and stir at a speed of 260 r / min for 60 min to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of 41:1.3:0.7:0.3:0.8, and stir at a speed of 120 r / min for 20 min to obtain cyanobacteria organic fertilizer.

[0061] Among them, the cyanobacteria extract is prepared by the following method: S610: Air-dry cyanobacteria at a temperature of 60 °C, and then put them into a grinder to grind into cyanobacteria powder; S620: Mix cyanobacteria powder, citric acid, and water with a mass ratio of 1:2.3:18, and then perform water extraction at a temperature of 65 °C for 3 h. After filtration, obtain a first filtrate and a second filtrate; S630: Mix the first filtrate and sodium hydroxide with a mass ratio of 1.2:0.05, and then perform water extraction at a temperature of 65 °C for 1.5 h. After filtration, obtain a second filtrate and a second filter residue; S640: Mix the first filter residue, the second filter residue, cellulase, and water with a mass ratio of 1.03:0.02:0.03:7, and then heat to 55 °C and perform enzymatic hydrolysis for 4.5 h to obtain a cyanobacteria enzymatic hydrolysate; S650: Mix the cyanobacteria enzymatic hydrolysate, magnesium sulfate, and sodium chloride with a mass ratio of 8:0.07:0.1, and then let it stand for 7 days to obtain a fermentation material; S660: Mix the fermentation material and the second filtrate with a mass ratio of 1:1 to obtain a cyanobacteria extract.

[0062] Comparative example 1: It is the commercially available Xin Hongqiao medium element water-soluble fertilizer.

[0063] Comparative example 2: Water group Performance test Prepare 12 test fields, all planted with Chinese cabbages, and apply the cyanobacteria organic fertilizers prepared in Examples 1 to 10 of the present invention, as well as the commercially available water-soluble fertilizer in Comparative Example 1 and the water in Comparative Example 2. The application amount per test field is 500 kg / hm. After one month, harvest the Chinese cabbages.

[0064] For the Chinese cabbages harvested from each experimental plot, fresh sample quality index analysis was carried out. Among them, the vitamin C content was determined by the 2,6-dichlorophenol indophenol titration method, the nitrate content was determined by the salicylic acid colorimetric method, the soluble sugar content was determined by the anthrone colorimetric method, and the soluble protein content was determined by the Coomassie brilliant blue staining method. The test results are shown in Table 1.

[0065] Table 1

[0066] It can be seen from the data recorded in Table 1 that the cyanobacteria organic fertilizer of the present invention can effectively improve the nutrient content of Chinese cabbages, and has a better improvement effect compared with the commercially available water-soluble fertilizers.

[0067] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be subject to the scope defined by the claims.

Claims

1. A preparation process of a cyanobacteria organic fertilizer, characterized in that, Including: S100: Mix humic acid, monoammonium phosphate, and potassium sulfate to obtain a first mixture; S200: Mix the first mixture and a blue-green algae extract to obtain a second mixture; S300: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium to obtain a third mixture; S400: Mix peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate to obtain a fourth mixture; S500: Add the fourth mixture to the third mixture to obtain a blue-green algae organic fertilizer.

2. The preparation process according to claim 1, characterized in that, The S100 specifically includes the following steps: Add humic acid, monoammonium phosphate, and potassium sulfate with a mass ratio of (8 - 12):(4 - 8):(5 - 10) to a stirrer, and then stir at a speed of 180 r / min - 240 r / min for 20 min - 40 min to obtain the first mixture.

3. The preparation process according to claim 1, characterized in that, The S200 specifically includes the following steps: Add the first mixture and the blue-green algae extract with a mass ratio of (15 - 30):(15 - 20) to a stirrer, and then stir at a speed of 200 r / min - 300 r / min for 40 min - 80 min to obtain the second mixture.

4. The preparation process according to claim 1, characterized in that, The S300 specifically includes the following steps: Mix the second mixture, sodium alginate, chelated zinc, chelated iron, and chelated magnesium with a mass ratio of (30 - 50):(1 - 2):(0.6 - 0.8):(0.2 - 0.4):(0.7 - 0.9), and then stir at a speed of 100 r / min - 150 r / min for 15 min - 25 min to obtain the third mixture.

5. The preparation process according to claim 4, characterized in that, The blue-green algae extract is prepared by the following method: S610: Dry and grind blue-green algae to obtain blue-green algae powder; S620: Take the blue-green algae powder for a first water extraction to obtain a first filtrate and a first filter residue; S630: Perform a second water extraction on the first filtrate to obtain a second filtrate and a second filter residue; S640: Add the first filter residue and the second filter residue to water, and then add cellulase thereto. After enzymatic hydrolysis, a blue-green algae enzymatic hydrolysate is obtained; S650: Add magnesium sulfate and sodium chloride to the blue-green algae enzymatic hydrolysate, and let it stand for 5 - 10 days to obtain a fermentation material; S660: Mix the fermentation material and the second filtrate to obtain the blue-green algae extract.

6. The preparation process according to claim 5, characterized in that, In the S620, mix the blue-green algae powder, citric acid, and water with a mass ratio of 1:(2 - 3):(15 - 20), and then perform water extraction at a temperature of 60°C - 70°C for 2 h - 4 h. After filtration, the first filtrate and the second filtrate are obtained.

7. The preparation process according to claim 5, characterized in that, In the S630, mix the first filtrate and sodium hydroxide with a mass ratio of (1 - 1.5):(0.02 - 0.1), and then perform water extraction at a temperature of 60°C - 70°C for 1 h - 2 h. After filtration, the second filtrate and the second filter residue are obtained.

8. The preparation process according to claim 5, characterized in that, In the S640, the primary filter residue, the secondary filter residue, cellulase, and water with a mass ratio of (1 - 1.1):(0.01 - 0.03):(0.02 - 0.05):(5 - 8) are mixed, and then heated to 50°C - 60°C and enzymatically hydrolyzed for 4 h - 5 h to obtain the cyanobacteria enzymatic hydrolysate.

9. The preparation process according to claim 1, characterized in that, The S400 specifically includes the following steps: Peroxidized humic acid, magnesium sulfate, and potassium dihydrogen phosphate with a mass ratio of (1 - 2):(5 - 10):(4 - 8) are mixed, and then left standing for 40 min - 60 min to obtain the fourth mixture.

10. The preparation process according to claim 1, characterized in that, The S500 specifically includes the following steps: The third mixture and the fourth mixture with a mass ratio of (30 - 55):(1 - 2) are mixed, and then stirred at a speed of 60 r / min - 100 r / min for 5 min - 15 min to obtain the cyanobacteria organic fertilizer.

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