Feed triticale seed treatment method

Abstract

The invention relates to a feed triticale seed treatment method, and belongs to the technical field of agricultural planting. By using a compound medicament of uniconazole, humic acid chelated zinc and betaine, the germination rate, the lodging resistance and the tiller number of the feed triticale in a saline-alkali environment are remarkably improved. Experiments show that under the stress of 0.5% salt and alkali, the germination rate of an all-component optimized group reaches 83.3%, the proline content reaches 32.7 mu mol / g, the chlorophyll SPAD value is 36.5, the sodium-potassium ratio (Na < + > / K < + >) is 0.43, the tiller number is 5.5 per plant, and the breaking strength of stalks is 38.5 N. According to the method, the optimal proportion of uniconazole 30 mg / L, humic acid chelated zinc 0.03% and betaine 0.05% is determined, and the obvious synergistic interaction effect is achieved.

Description

TECHNICAL FIELD

[0001] The application relates to a triticale seed processing method and belongs to the technical field of agricultural planting. BACKGROUND

[0002] Uniconazole, as a plant growth regulator, can affect plant growth by regulating the biosynthesis of gibberellins. When triticale is planted in saline-alkali soil, using uniconazole dry seed treatment and other treatment methods can promote root development, increase the number of roots, root activity, root dry weight, total root length and other indicators, and thus improve the salt tolerance and yield of triticale. Liu Huimin et al. of the Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences found that uniconazole 75 mg / kg seed dressing can effectively improve the salt tolerance of wheat, and the plant height, fresh weight and dry weight are 9.5%, 37.5% and 46.5% higher than those of the control, respectively. The Na + and Cl - contents of the aboveground part and the root are 16.2%, 18.7% and 15.9%, 15.0% lower than those of the control, respectively, thereby alleviating the harm of salt stress.

[0003] Humic acid in humic acid chelated zinc has the effects of improving soil structure, regulating soil pH, increasing soil water and fertilizer retention capacity, and is helpful to improve the soil environment of saline-alkali soil and create more favorable conditions for the growth of triticale. At the same time, zinc is one of the trace elements necessary for plant growth, and humic acid chelated zinc can provide zinc nutrition for triticale, promote its growth and development, and improve the quality and yield of crops. Studies have shown that when using humic acid chelated zinc and other formulations to improve different concentrations of saline-alkali soil, the total salt content and alkalization degree of the soil decrease, the soil available potassium, available nitrogen and slow-release potassium content increase, the soil bulk density decreases, the soil porosity increases, and the crop yield also increases.

[0004] Betaine has physiological functions such as regulating plant cell osmotic pressure, stabilizing biological membrane system and participating in signal transduction, and can enhance the stress resistance of triticale, so that it can better maintain the stability and integrity of cells in the saline-alkali environment and reduce the damage of salt stress to plant cells. Exogenous spraying of betaine can increase the chlorophyll content, free proline content in plant leaves, enhance the activity of protective enzymes such as superoxide dismutase and peroxidase, and increase the soluble sugar content, thereby alleviating the damage of salt stress to plants, improving the photosynthetic rate and water use efficiency of plants, and promoting plant growth.

[0005] Although the effects of uniconazole, humic acid chelated zinc and betaine on improving the salt tolerance of triticale are known to some extent, the specific mechanism of their interaction and how to synergistically regulate the growth and development process of triticale in saline-alkali soil still need further research.

[0006] Different degrees of soil salinity, climate conditions, and triticale varieties can affect the use of uniconazole, humic acid chelated zinc, and betaine. However, a best combination use scheme suitable for various conditions, including their use concentration, use time, and application method, has not yet been determined. For example, different amounts and use frequencies may be needed to achieve the best planting effect in light and heavy saline-alkali soils. SUMMARY

[0007] A method for treating forage triticale seeds suitable for planting in saline-alkali soil, characterized in that it comprises the following steps: using a compound agent of uniconazole, humic acid chelated zinc, and betaine to treat forage triticale seeds.

[0008] The concentrations of the components in the agent are: uniconazole 20-40 mg / L, humic acid chelated zinc 0.01%-0.04%, and betaine 0.01%-0.1%. The concentrations of the components in the agent are: uniconazole 30 mg / L, humic acid chelated zinc 0.03%, and betaine 0.05%.

[0009] The treatment method further comprises seed disinfection and drying steps.

[0010] A compound agent for treating forage triticale seeds suitable for planting in saline-alkali soil, characterized in that it is composed of uniconazole, humic acid chelated zinc, and betaine, and an adjuvant.

[0011] The concentrations of the components in the agent are: uniconazole 20-40 mg / L, humic acid chelated zinc 0.01%-0.04%, and betaine 0.01%-0.1%.

[0012] The concentrations of the components in the agent are: uniconazole 30 mg / L, humic acid chelated zinc 0.03%, and betaine 0.05%.

[0013] The compound agent can be used alone or prepared into a suitable dosage form.

[0014] The suitable dosage forms are seed treatment microcapsule suspensions and suspension seed coatings.

[0015] The seed treatment microcapsule suspension agent mainly comprises effective components, capsule core solvent, capsule wall material (oily), capsule wall material (aqueous), emulsifier, dispersant, antifreezing agent, thickening agent, dyeing agent, film forming agent, deionized water. The capsule core solvent mainly comprises toluene, xylene, solvent oil S-150, solvent oil S-200, ethanol, isopropyl alcohol, n-butyl alcohol, n-octyl alcohol, n-amyl alcohol, acetone, cyclohexanone, hexane, dimethylformamide, N-methyl pyrrolidone, acetonitrile, dichloromethane, ethyl acetate, methyl oleate, dibutyl phthalate, etc. The capsule wall material (oily) mainly comprises diphenyl methane diisocyanate, hexamethylene diisocyanate, 4, 4'-dicyclohexyl methane diisocyanate, etc. The capsule wall material (aqueous) mainly comprises ethylene glycol, glycerol, ethylenediamine, propylenediamine, propylenetriamine, water, etc. containing polyhydroxy or polyamine groups or compounds generated by reacting with oily monomers containing the above two groups. The emulsifier mainly comprises agricultural emulsifier 33#, agricultural emulsifier 34#, agricultural emulsifier 500#, agricultural emulsifier 600#, agricultural emulsifier 700#, agricultural emulsifier 1601#, agricultural emulsifier 1602#, T60, S80, TX-10, OP-10, NP-10, etc. The dispersant mainly comprises dispersant NNO, sodium lignosulfonate, calcium lignosulfonate, dispersant 2700, polycarboxylate dispersant, phosphate dispersant, etc. The antifreezing agent mainly comprises ethylene glycol, propylene glycol, glycerol, urea, sodium chloride, etc. The thickening agent is xanthan gum, polyvinyl alcohol, magnesium aluminum silicate, gum arabic, etc. The film forming agent mainly comprises polysaccharide polymer compounds, cellulose derivatives, alginate, agar, rosin, paraffin, gelatin, pectin, polyvinyl alcohol, polyethylene glycol, polyvinyl acetate, polyacrylamide, polyvinylpyrrolidone and polyol polymer water-soluble synthetic products, etc. The defoaming agent mainly comprises organosilicon and higher alcohols, etc. The dyeing agent mainly comprises rose red, magenta, scarlet yellow, blue, purple, etc.

[0016] For the flowable concentrate, the adjuvants that can be used include one or more of the following: a binder such as a polysaccharide polymer compound (soluble starch, polypropylene graft copolymer, xanthan gum, microbial mucilage), a cellulose derivative (hydroxypropyl methyl cellulose, sodium carboxymethyl cellulose, ethyl cellulose), a seaweed such as sodium alginate, agar, rosin, paraffin, gelatin, pectin, polyvinyl alcohol, polyethylene glycol, polyvinyl acetate, polyacrylamide, polyvinylpyrrolidone, and a water-soluble synthetic polyol polymer, an inorganic binder (magnesium aluminum silicate, clay, water glass, gypsum); a dispersing agent such as polycarboxylate, lignin sulfonate, alkyl naphthalene sulfonate, TERSPERSE 2020 (a product of Huntsman Corporation, USA, alkyl naphthalene sulfonate); an emulsifying agent such as AGRIMUL 700# (common name: alkyl phenol formaldehyde resin polyoxyethylene ether), AGRIMUL 2201, SPAN-60# (common name: sorbitan monostearate), EMULSIFIER T-60 (common name: sorbitan monostearate polyoxyethylene ether), AGRIMUL 1601# (common name: phenylethyl phenol polyoxyethylene polyoxypropylene ether), TERSPERSE 4894 (a product of Huntsman Corporation, USA); a wetting agent such as alkyl phenol polyoxyethylene ether formaldehyde condensate sulfate, alkyl phenol polyoxyethylene ether phosphate, phenylethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate, TERSPERSE 2500 (a product of Huntsman Corporation, USA); a thickening agent such as xanthan gum, polyvinyl alcohol, bentonite; a preservative such as formaldehyde, benzoic acid, sodium benzoate; an antifoaming agent such as silicone antifoaming agent; an antifreeze agent such as ethylene glycol, propylene glycol, glycerol, urea, an inorganic salt such as sodium chloride; a warning colorant such as one of the following colors: rose, magenta, scarlet yellow, blue, violet; and water is deionized water.

[0017] A method for improving the salt-alkali resistance of a forage triticale, characterized in that the method comprises treating the seeds of the forage triticale with the compound preparation.

[0018] A method for improving the salt-alkali resistance of a forage triticale, characterized in that the method comprises treating the seeds of the forage triticale with a suitable dosage form prepared from the compound preparation.

[0019] The suitable dosage form for preparation is a seed treatment microcapsule suspension concentrate or a flowable concentrate.

[0020] The treatment method further comprises a seed disinfection and drying step.

[0021] The beneficial effects that can be produced by the present application include:

[0022] The application relates to a forage triticale seed treatment method suitable for saline-alkali land planting, which uses an enzofuracarb, humic acid chelated zinc and betaine compound agent to treat forage triticale seeds.

[0023] The application achieves a germination rate of 83.3% (35.9% higher than that of a saline-alkali control) under 0.5% NaCl stress, a proline accumulation amount of 32.7+2.5 mu mol / g (77.7% higher than that of a single deficiency group), an optimized sodium-potassium ratio of 0.43+0.05 (61.6% reduction), a stem base internode shortening to 3.8+0.3 cm (26.9% reduction), a bending resistance of 38.5+2.1 N (55.9% increase) and a tiller number of 5.5+0.6 per plant (77.4% increase).

[0024] The application relates to a forage triticale seed treatment method suitable for saline-alkali land planting, which can improve the germination rate of forage triticale planted in saline-alkali land, improve biological indexes and is suitable for forage triticale planting in saline-alkali land. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 Forage triticale seed germination treatment diagram

[0026] Figure 2 Forage triticale seed germination treatment diagram

[0027] Figure 3 Forage triticale seed germination treatment diagram

[0028] Figure 4 Forage triticale seed germination treatment diagram DETAILED DESCRIPTION

[0029] The application examples are described in detail below.

[0030] If the specific experimental steps or conditions are not indicated in the examples, the operation or conditions can be carried out according to the conventional experimental steps described in the literature in the field. If the reagents or instruments are not indicated by the manufacturers, they are all conventional reagent products that can be obtained on the market.

[0031] Reagent sources:

[0032] Reagent name Specification / purity Manufacturer Uniconazole Analytical pure ≥ 98% Sigma-Aldrich Humic acid chelated zinc Zinc content ≥ 15% Beijing Zhongnong Ke Lian Biology Betaine Biochemical reagent ≥ 99% Aladdin Biochemical

[0033] I. Experimental scheme

[0034] 1. Experimental objective

[0035] Test the effect of uniconazole + humic acid chelated zinc + betaine complexing agent on lodging resistance, tillering number, germination rate and salt resistance index of forage triticale under saline-alkali stress.

[0036] 2、Experimental grouping

[0037]

[0038] II. Simulation of saline-alkali stress and salt resistance index

[0039] 1. Preparation of saline-alkali soil

[0040] Simulated saline-alkali soil: Mix NaCl (salt) and NaHCO3 (alkali) according to the mass ratio to prepare soil containing 0.3%, 0.5%, and 0.7% salt (pH 8.3-8.5), referring to the parameters of saline-alkali land in the Yellow River Delta.

[0041] 2. Seed treatment

[0042] Jinmicao No. 1 seeds were disinfected with 0.1% HgCl2 for 10 minutes and rinsed with distilled water three times. The seed-to-liquid ratio was 1:3, and the seeds were incubated at 25°C for 12 hours with constant temperature shaking (60 Hz). After draining, the seeds were dried at 40°C with hot air until the moisture content was 15%. The dried seeds were evenly sown in petri dishes (15 cm in diameter) containing simulated saline-alkali soil, with a sowing depth of 2 cm. Each group had three replicates, and 50 seeds were sown in each dish.

[0043] 3. Germination condition control: temperature: 25±1°C constant temperature incubator; humidity: maintain soil relative water content at 70%-75% (weigh daily and add water); light: 12h / 12h light-dark cycle, light intensity 200μmol·m -2 ·s -1 .

[0044] 4. Germination monitoring: record the number of germinated seeds (embryonic root breaking through seed coat ≥2mm is considered germination) daily; calculate the germination rate on the 7th day (GB / T 3543.4-1995 standard).

[0045] Maintain soil moisture content at 60%-65% (avoid leaching in saline-alkali stress groups), and do not add fertilizer during the entire growth period (to exclude nutrient interference). Tillering number was counted at the jointing stage (35 days), and stem bending resistance was measured at the heading stage (60 days).

[0046] 5. Main indicators and detection methods

[0047] Index Detection method Germination rate GB / T 3543.4-1995 Root length / height ratio Vernier caliper measurement Proline content Sulfo-salicylic acid method (μmol / g) Chlorophyll SPAD value Acetone extraction method Sodium-potassium ratio (Na + / K + )]]> Flame photometry determination

[0048] III. Germination rate and salt resistance index under saline-alkali stress

[0049] 1. Experimental data of triticale seed germination rate

[0050] Group Salt concentration % Germination rate Group 1 germination number Group 2 germination number Group 3 germination number Whole component optimization group 0.3 91.33％ 45 47 45 0.5 83.33％ 41 41 43 0.7 78.67％ 39 39 40 Uniconazole deficiency group 0.5 78.00％ 39 40 38 Humic acid chelated zinc deficiency group 0.5 77.33％ 38 38 40 Betaine deficiency group 0.5 69.33％ 34 35 35 Saline-alkali control group 0.3 71.33％ 35 36 36 0.5 61.33％ 31 31 30 0.7 48.00％ 24 22 26 Non-saline-alkali control group 0 96.67％ 48 48 49

[0051] 2. Apparent salt-alkaline resistance index

[0052]

[0053]

[0054] The number of effective tillers in the whole group was 5.5±0.6 per plant (3.1±0.4 per plant in the salt-alkaline control), and the number of effective tillers in the group lacking humic acid chelated zinc was reduced to 3.8±0.5 (P<0.05). The length of the basal internode was shortened to 3.8±0.3 cm (5.2±0.4 cm in the control) by the use of uniconazole, and the bending strength was increased to 38.5±2.1 N (24.7±1.8 N in the control).

[0055] 3. Physiological and biochemical salt-alkaline resistance index

[0056]

[0057] Betaine maintains the stability of the cell membrane, and the accumulation of proline was increased by 77.7% (32.7 vs 18.4 μmol / g) compared with the single deficiency group. The use of humic acid chelated zinc increased the chlorophyll content by 18.3%, and the photosynthetic rate reached 22.4 (18.9 μmol·m-2·s-1 in the group lacking humic acid chelated zinc).

[0058] Uniconazole inhibits gibberellin synthesis, up-regulates SOD activity, and alleviates oxidative damage under salt stress. Humic acid chelated zinc chelates free Na + Betaine acts as an osmotic protectant to improve the germination rate. The present application relates to a seed treatment method for triticale for planting in saline-alkali soil, which can improve the germination rate of triticale planted in saline-alkali soil and improve the biological index of triticale, and is suitable for planting triticale in saline-alkali soil.

[0059] In the present specification, the same or similar parts among the various embodiments are cross-referenced, and each embodiment focuses on the differences from other embodiments. In particular, for the embodiments described later, the description is relatively simple, and the relevant parts are cross-referenced with the part of the foregoing embodiments.

[0060] The above merely illustrates the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of changes or replacements within the technical range disclosed in the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A method for treating forage triticale seeds suitable for planting in saline-alkali land, characterized in that, Includes the following steps: Forage rye seeds were treated with a compound agent consisting of uniconazole, zinc humate chelate, and betaine.

2. The method for treating forage triticale seeds suitable for planting in saline-alkali land according to claim 1, characterized in that, The concentrations of each component in the reagent are as follows: uniconazole 20-40 mg / L, humic acid chelated zinc 0.01%-0.04%, and betaine 0.01%-0.1%.

3. The method for treating forage triticale seeds suitable for planting in saline-alkali land according to claim 2, characterized in that, The concentrations of each component in the reagent are as follows: uniconazole 30 mg / L, humic acid chelated zinc 0.03%, and betaine 0.05%.

4. The method for treating feed rye seeds according to claim 3, characterized in that, The treatment method also includes seed disinfection and drying steps.

5. A compound agent for treating forage rye seeds suitable for planting in saline-alkali land, characterized in that, It is composed of uniconazole, humic acid chelated zinc, betaine, and adjuvants.

6. The compound agent for treating forage triticale seeds suitable for planting in saline-alkali land according to claim 5, characterized in that, The concentrations of each component in the reagent are as follows: uniconazole 20-40 mg / L, humic acid chelated zinc 0.01%-0.04%, and betaine 0.01%-0.1%.

7. The compound agent for treating forage triticale seeds suitable for planting in saline-alkali land according to claim 6, characterized in that, The concentrations of each component in the reagent are as follows: uniconazole 30 mg / L, humic acid chelated zinc 0.03%, and betaine 0.05%.

8. A method for improving the salt and alkali resistance of feed rye, characterized in that, This includes treating feed rye seeds with the compound agents described in claims 5-7.

9. The method for improving the salt and alkali resistance of feed rye according to claim 8, characterized in that, The treatment method also includes seed disinfection and drying steps.

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