Method for improving properties of poplar

The GLR3.3a and GLR3.3b genes of poplar trees were knocked out through the CRISPR/Cas9 system to regulate the traits of poplar trees, solve the problems of the developed root system and plant height of poplar trees, and improve its stability and wood quality in desert or soft soil.

CN120400155APending Publication Date: 2025-08-01ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Application Number
CN202510558412.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2022-09-19
Filing Date
2023-05-23
Publication Date
2025-08-01

AI Technical Summary

Technical Problem

The prior art lacks effective methods to increase the degree of root development of poplar trees and reduce plant height, affecting their stability and wood quality in desert or soft soil.

Method used

The GLR3.3a and GLR3.3b genes in poplar trees were knocked out through the CRISPR/Cas9 system, combined with genetic engineering vectors and recombinant Agrobacterium, and the expression of related genes in poplar trees was regulated, thereby increasing the width of xylem, cellulose content and root development.

Benefits of technology

The number, length and cross-sectional area of poplar trees have been significantly increased, the plant height has been reduced, the fixed soil and wind resistance of the root system has been improved, and the strength and tensile properties of the wood are enhanced.

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Abstract

The invention discloses a method for improving the character of a poplar, which is characterized in that a poplar GLR3.3a gene and a poplar GLR3.3b gene are knocked out through a CRISPR / Cas9 system to obtain a gene knockout poplar. Compared with a wild type poplar, the widths of a gelatinous layer, xylem in a stem, a cambium and a cortex are increased in a stem cell wall of the gene knockout poplar, and the cellulose content is remarkably improved, so that wood has higher strength and tensile strength, and the mechanical property of the poplar is improved.
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