Microfluidic detection device and use method thereof

Abstract

The invention discloses a microfluidic detection device and a use method thereof, and relates to the technical field of microfluidic detection.The microfluidic detection device comprises a detection device body, a movable plate is movably connected to the side face of the detection device body, a microfluidic chip is arranged on the inner side of the movable plate, a movable frame is rotationally connected to the upper portion of the detection device body, and an adjusting mechanism is arranged on the movable frame; the adjusting mechanism comprises a first servo motor, a second servo motor and an L-shaped plate, the first servo motor and the second servo motor are installed on the movable frame, the L-shaped plate is movably connected to the outer side of the movable frame, an opening groove is formed in the outer side of the L-shaped plate, and a control system is arranged on the movable frame. And then the adjusting mechanism is controlled to drive the L-shaped plate to move, so that the L-shaped plate is aligned with the injection port required by a user, the pipette can be ensured to be more accurately aligned with the micro-fluidic injection port, and the problems of slow alignment, liquid splashing, dripping position deviation and the like due to manual operation are avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic detection, and particularly to a microfluidic detection device and a method for using the same. Background Art

[0002] A microfluidic detection device is a detection tool based on microfluidic technology. It uses tiny channels or chambers to manipulate and measure fluid flow rates, and is typically used for precise fluid control and measurement within a space ranging from micrometers to nanometers. A microfluidic detection device can achieve efficient, sensitive, and accurate biomolecule detection and analysis. It utilizes the property that fluorescently labeled biomolecules emit fluorescence under the irradiation of excitation light for detection. Then, by designing a specific microchannel structure, a sample to be tested is introduced into the microfluidic chip and combined with the fluorescently labeled probe molecules. Under the irradiation of excitation light, the change in the fluorescence signal is observed, thereby achieving qualitative and quantitative analysis of biomolecules.

[0003] When a user introduces a sample to be tested into the injection port of a microfluidic chip through a pipette, the user needs to observe the position of the injection port with their eyes and then manually align the tip of the pipette above the injection port. Since the injection port is small, the alignment process takes time and is prone to liquid splashing and dripping position deviation due to unstable operation. Therefore, a microfluidic detection device and a method for using the same are proposed. Summary of the Invention

[0004] The purpose of the present invention is to solve the drawbacks in the prior art that the user observes the position of the injection port with their eyes and then manually aligns the tip of the pipette above the injection port. Since the injection port is small, the alignment process takes time and is prone to liquid splashing and dripping position deviation due to unstable operation, and to propose a microfluidic detection device and a method for using the same.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A microfluidic detection device includes a detection device. A movable plate is movably connected to the side of the detection device. A microfluidic chip is disposed inside the movable plate. A movable frame is rotatably connected to the upper part of the detection device. An adjustment mechanism is provided on the movable frame. The adjustment mechanism includes a first servo motor and a second servo motor installed on the movable frame, and an L-shaped plate movably connected to the outside of the movable frame. An opening groove is formed on the outside of the L-shaped plate. A control system is provided on the movable frame. The control system includes:

[0007] An image acquisition module for capturing images of the microfluidic chip and its injection port and transmitting them to the image processing module;

[0008] An image processing module, which is used to process the images collected by the image acquisition module, extract the feature information of the injection ports, and identify the positions and features of all injection ports on the microfluidic chip;

[0009] An image recognition module, which matches the feature information extracted by the image processing module with a preset injection port feature library through a feature matching algorithm to determine the identity of each injection port;

[0010] A position feedback and adjustment module, which is used to feedback the position information of the injection port selected by the user to the first servo motor and the second servo motor, and control the first servo motor and the second servo motor to adjust the position of the L-shaped plate through a path planning algorithm;

[0011] A human-computer interaction module, which is used to provide a user operation interface and allow the user to select a target injection port;

[0012] After the microfluidic chip is placed in the movable plate, the image acquisition module captures the images of the microfluidic chip and its injection ports and transmits them to the image processing module. The image processing module preprocesses the images and performs target detection, identifies the positions and features of all injection ports and transmits the feature information to the image recognition module. The image recognition module confirms the identity of the injection ports recognized by the image processing module and determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module. The position feedback and adjustment module feedbacks the user's selection to the first servo motor and the second servo motor. The first servo motor and the second servo motor are started to drive the L-shaped plate to move in the horizontal position until the opening groove corresponds to the position of the injection port, and then the pipette is inserted into the opening groove for guiding and dropping liquid into the microfluidic chip, which can ensure that the pipette is more accurately aligned with the injection port of the microfluidic chip, avoid slow positioning caused by manual operation, and also help to reduce problems such as liquid splashing and dripping position deviation caused by unstable operation.

[0013] The above technical solution further includes:

[0014] The inner sides of the movable frame are respectively rotatably connected with a first threaded rod and a guide rod. The first threaded rod is installed at the end of the output shaft of the first servo motor. An movable rod is slidably arranged on the outer side of the guide rod. The movable rod is threadedly connected with the first threaded rod. When the first threaded rod rotates, it drives the movable rod to move along the guide rod.

[0015] Square grooves and sliding grooves are respectively formed at the bottom of the movable rod. The second servo motor is installed inside the square groove. A sliding block is slidably arranged inside the sliding groove, and the sliding block moves along the sliding groove.

[0016] One end of the second servo motor extending to the inner side of the sliding groove is equipped with a second threaded rod, which is threadedly connected to the sliding block. The sliding block is fixedly connected to the L-shaped plate. Rotation of the second threaded rod can drive the sliding block to move along the sliding groove.

[0017] The size of the opening of the sliding groove is adapted to the size of the sliding block. The cross-section of the sliding groove and the sliding block is convex to ensure the stability of the sliding of the sliding block.

[0018] The image acquisition module includes an image capture unit and a data transmission unit. The image capture unit captures images of the microfluidic chip and its injection ports through a camera and converts them into digital signals. The data transmission unit transmits the captured image data to the image processing module in real time.

[0019] The image processing module includes an image preprocessing unit, a feature extraction unit, and a target detection unit. The image preprocessing unit performs preprocessing operations of denoising and enhancement on the captured images. The feature extraction unit extracts the feature information of the injection ports from the preprocessed images. The target detection unit identifies the positions and features of all injection ports on the microfluidic chip.

[0020] The image recognition module includes a feature matching unit and a position accuracy correction unit. The feature matching unit matches the extracted feature information with a preset injection port feature library through a feature matching algorithm to determine the identity of each injection port. The position accuracy correction unit performs accuracy correction on the positions of the matched injection ports to improve the positioning accuracy;

[0021] The feature matching algorithm is the Euclidean distance, which is used to measure the similarity between two feature points. The smaller the Euclidean distance, the more similar the two feature points are;

[0022] The calculation formula of the Euclidean distance is:

[0023]

[0024] where f1 and f2 are the descriptors of two feature points, f 1i and f 2i are the i-th eigenvalue, and n is the dimension of the feature vector.

[0025] The position feedback and adjustment module includes a path planning unit, a control unit, and a position monitoring unit. The path planning unit calculates the movement path of the L-shaped plate according to the position information of the target injection port through a path planning algorithm. The control unit controls the operation of the first servo motor and the second servo motor. The position monitoring unit uses a position sensor to monitor the actual position of the L-shaped plate in real time. The path planning algorithm is the A* algorithm, which is used to find the shortest path from the starting point to the target point in a graphical environment. Its algorithm formula is:

[0026] Total path cost F(n): F(n) = G(n) + H(n);

[0027] G(n): The actual cost from the starting point to the current node n;

[0028] H(n): The estimated cost from the current node n to the target point.

[0029] A method used in a microfluidic detection device, which adopts a microfluidic detection device, and includes the following steps:

[0030] Step 1: First, pull the movable plate to move the movable plate to the outside of the detection device, then place the microfluidic chip into the groove of the movable plate, and then rotate the movable frame until the movable frame is parallel to the movable plate;

[0031] Step 2: The image acquisition module captures images of the microfluidic chip and its injection ports, then the image processing module identifies the positions and features of all injection ports, and then the image recognition module determines the accurate positions of the target injection ports. The user selects the target injection port through the human-machine interaction module according to the needs, and the position feedback and adjustment module feeds back the injection port position information selected by the user to the first servo motor and the second servo motor;

[0032] Step 3: After receiving the signal, the first servo motor and the second servo motor are started to drive the L-shaped plate to move in the horizontal position until the opening groove corresponds to the position of the injection port. Then, the staff inserts the pipette into the opening groove for guidance and drops the liquid into the microfluidic chip. After the liquid dropping is completed, the movable plate is closed, and the detection is carried out through the detection device.

[0033] The present invention has the following beneficial effects:

[0034] 1. In the present invention, by setting up a control system, the position of the injection port of the microfluidic chip can be recognized in real time, and then the control adjustment mechanism is controlled to drive the L-shaped plate to move, so that the L-shaped plate is aligned with the position of the injection port required by the user, which can ensure that the pipette is more accurately aligned with the injection port of the microfluidic, avoiding slow alignment during manual operation, and also helping to reduce problems such as liquid splashing and dripping position deviation caused by unstable operation. Description of the Drawings

[0035] Figure 1 It is a schematic diagram of the overall structure of a microfluidic detection device and its use method proposed by the present invention;

[0036] Figure 2 It is a schematic diagram of the structure of the movable frame in the present invention;

[0037] Figure 3 It is a schematic diagram of the control system in the present invention;

[0038] Figure 4 For Figure 1 the enlarged schematic diagram of the structure at position A in

[0039] Figure 5 For Figure 2 the enlarged schematic diagram of the structure at position B in

[0040] Figure 6 For Figure 2 the enlarged schematic diagram of the structure at position C in

[0041] In the figure: 1. Detection device; 2. Movable plate; 3. Microfluidic chip; 4. Movable frame; 5. First servo motor; 6. First threaded rod; 7. Guide rod; 8. Movable rod; 9. Second servo motor; 10. Sliding groove; 11. Second threaded rod; 12. Sliding block; 13. L-shaped plate; 14. Open slot; 15. Square slot; 16. Image acquisition module; 17. Image processing module; 18. Image recognition module; 19. Position feedback and adjustment module; 20. Human-computer interaction module. Specific embodiments

[0042] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0043] As Figure 1 - Figure 6 shown, a microfluidic detection device proposed by the present invention includes a detection device 1. A movable plate 2 is movably connected to the side of the detection device 1. A microfluidic chip 3 is arranged inside the movable plate 2. A movable frame 4 is rotatably connected to the upper part of the detection device 1. An adjustment mechanism is arranged on the movable frame 4. The adjustment mechanism includes a first servo motor 5 and a second servo motor 9 installed on the movable frame 4, and an L-shaped plate 13 movably connected to the outside of the movable frame 4. An open slot 14 is opened on the outside of the L-shaped plate 13. A control system is arranged on the movable frame 4. The control system includes:

[0044] An image acquisition module 16 for capturing images of the microfluidic chip 3 and its injection ports and transmitting them to the image processing module 17;

[0045] An image processing module 17 for processing the images collected by the image acquisition module 16, extracting the characteristic information of the injection ports, and identifying the positions and characteristics of all the injection ports on the microfluidic chip 3;

[0046] The image recognition module 18 matches the feature information extracted by the image processing module 17 with the preset injection port feature library through a feature matching algorithm to determine the identity of each injection port;

[0047] The position feedback and adjustment module 19 is used to feedback the position information of the injection port selected by the user to the first servo motor 5 and the second servo motor 9, and controls the first servo motor 5 and the second servo motor 9 to adjust the position of the L-shaped plate 13 through a path planning algorithm;

[0048] The human-computer interaction module 20 is used to provide a user operation interface and allow the user to select a target injection port;

[0049] After the microfluidic chip 3 is placed in the movable plate 2, the image acquisition module 16 captures the images of the microfluidic chip 3 and its injection ports and transmits them to the image processing module 17. The image processing module 17 preprocesses the images and performs target detection, identifies the positions and features of all injection ports and transmits the feature information to the image recognition module 18. The image recognition module 18 confirms the identity of the injection ports recognized by the image processing module 17 and determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module 20. The position feedback and adjustment module 19 feeds back the user's selection to the first servo motor 5 and the second servo motor 9. The first servo motor 5 and the second servo motor 9 are started to drive the L-shaped plate 13 to move in the horizontal position until the opening slot 14 corresponds to the position of the injection port, and then the pipette is inserted into the opening slot 14 for guiding and dropping liquid into the microfluidic chip 3, which can ensure that the pipette is more accurately aligned with the injection port of the microfluidic chip 3, avoiding slow positioning caused by manual operation, and also helping to reduce problems such as liquid splashing and dripping position deviation caused by unstable operation.

[0050] The inner sides of the movable frame 4 are respectively rotatably connected with a first threaded rod 6 and a guide rod 7. The first threaded rod 6 is installed at the end of the output shaft of the first servo motor 5. An activity rod 8 is slidably arranged on the outer side of the guide rod 7. The activity rod 8 is threadedly connected with the first threaded rod 6. When the first threaded rod 6 rotates, it drives the activity rod 8 to move along the guide rod 7.

[0051] Square grooves 15 and sliding grooves 10 are respectively formed at the bottom of the activity rod 8. The second servo motor 9 is installed inside the square groove 15. A sliding block 12 is slidably arranged inside the sliding groove 10, and the sliding block 12 moves along the sliding groove 10.

[0052] One end of the second servo motor 9 extending to the inside of the sliding groove 10 is provided with a second threaded rod 11. The second threaded rod 11 is threadedly connected with the sliding block 12. The sliding block 12 is fixedly connected with the L-shaped plate 13. When the second threaded rod 11 rotates, it can drive the sliding block 12 to move along the sliding groove 10.

[0053] The size of the opening of the sliding groove 10 is adapted to the size of the sliding block 12. The cross-sections of the sliding groove 10 and the sliding block 12 are convex to ensure the stability of the sliding of the sliding block 12.

[0054] The image acquisition module 16 includes an image capture unit and a data transmission unit. The image capture unit captures images of the microfluidic chip 3 and its injection ports through a camera and converts them into digital signals. The data transmission unit transmits the captured image data to the image processing module 17 in real time.

[0055] The image processing module 17 includes an image preprocessing unit, a feature extraction unit, and a target detection unit. The image preprocessing unit performs preprocessing operations of denoising and enhancement on the captured images. The feature extraction unit extracts the feature information of the injection ports from the preprocessed images. The target detection unit identifies the positions and features of all injection ports on the microfluidic chip 3.

[0056] The image recognition module 18 includes a feature matching unit and a position accuracy correction unit. The feature matching unit matches the extracted feature information with a preset injection port feature library through a feature matching algorithm to determine the identity of each injection port. The position accuracy correction unit performs accuracy correction on the positions of the matched injection ports to improve the positioning accuracy;

[0057] The feature matching algorithm is the Euclidean distance. The Euclidean distance is used to measure the similarity between two feature points. The smaller the Euclidean distance, the more similar the two feature points are;

[0058] The calculation formula of the Euclidean distance is:

[0059]

[0060] where f1 and f2 are the descriptors of two feature points, f 1i and f 2i are the i-th eigenvalue, and n is the dimension of the feature vector.

[0061] The position feedback and adjustment module 19 includes a path planning unit, a control unit, and a position monitoring unit. The path planning unit calculates the movement path of the L-shaped plate 13 according to the position information of the target injection port through a path planning algorithm. The control unit controls the operation of the first servo motor 5 and the second servo motor 9. The position monitoring unit uses a position sensor to monitor the actual position of the L-shaped plate 13 in real time. The path planning algorithm is the A* algorithm, which is used to find the shortest path from the starting point to the target point in a graphical environment. Its algorithm formula is:

[0062] Total path cost F(n): F(n) = G(n) + H(n);

[0063] G(n): The actual cost of reaching the current node n from the starting point;

[0064] H(n): The estimated cost from the current node n to the target point.

[0065] In this embodiment, when detection work needs to be carried out, the microfluidic chip 3 is placed in the groove of the movable plate 2 at this time, and then the movable frame 4 is rotated until the movable frame 4 is parallel to the movable plate 2. At this time, the image acquisition module 16 can capture the image of the microfluidic chip 3 and its injection ports, and at the same time transmit the captured image to the image processing module 17. The image processing module 17 identifies the positions and features of all injection ports and transmits the feature information to the image recognition module 18. Then the image recognition module 18 confirms the identity of the injection ports identified by the image processing module 17 and determines the accurate position of the target injection port. At this time, the user can select the injection port that needs to be dripped at this time through the human-computer interaction module 20 according to the needs. The human-computer interaction module 20 transmits the selection information to the position feedback and adjustment module 19. The position feedback and adjustment module 19 then transmits the information of the injection port selected by the user to the first servo motor 5 and the second servo motor 9 and controls the first servo motor 5 and the second servo motor 9 to start;

[0066] After the first servo motor 5 starts, it can drive the first threaded rod 6 to rotate. The force generated when the first threaded rod 6 rotates drives the movable rod 8 to move along the guide rod 7, and at the same time drives the L-shaped plate 13 to move. After the second servo motor 9 starts, it can drive the second threaded rod 11 to rotate. The force generated when the second threaded rod 11 rotates can drive the slider 12 to move along the sliding groove 10, thereby driving the L-shaped plate 13 to move. In this way, the L-shaped plate 13 can be driven to move horizontally until the opening groove 14 opened on the L-shaped plate 13 corresponds to the position of the injection port selected by the user. At this time, the user can insert the pipette into the opening groove 14. Through the guidance of the opening groove 14, the muzzle of the pipette can be aligned with the injection port of the microfluidic chip 3, and then the dripping work can be carried out. After the dripping work is completed, at this time, the movable plate 2 can be pushed to push the movable plate 2 to the inside of the detection device 1, and then the detection work can be carried out through the detection device 1.

[0067] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microfluidic detection device, comprising a detection device (1), characterized in that, A movable plate (2) is movably connected to the side of the detection device (1). A microfluidic chip (3) is arranged inside the movable plate (2). A movable frame (4) is rotatably connected to the upper part of the detection device (1). An adjustment mechanism is arranged on the movable frame (4). The adjustment mechanism includes a first servo motor (5) and a second servo motor (9) installed on the movable frame (4), and an L-shaped plate (13) movably connected to the outside of the movable frame (4). An opening groove (14) is formed on the outside of the L-shaped plate (13). A control system is arranged on the movable frame (4). The control system includes: An image acquisition module (16) for capturing images of the microfluidic chip (3) and its injection ports and transmitting them to an image processing module (17); An image processing module (17) for processing the images acquired by the image acquisition module (16), extracting the characteristic information of the injection ports, and identifying the positions and characteristics of all injection ports on the microfluidic chip (3); An image recognition module (18) for matching the characteristic information extracted by the image processing module (17) with a preset injection port characteristic library through a feature matching algorithm to determine the identity of each injection port; A position feedback and adjustment module (19) for feeding back the position information of the injection port selected by the user to the first servo motor (5) and the second servo motor (9), and controlling the first servo motor (5) and the second servo motor (9) to adjust the position of the L-shaped plate (13) through a path planning algorithm; A human-computer interaction module (20) for providing a user operation interface and allowing the user to select a target injection port; After the microfluidic chip (3) is placed in the movable plate (2), the image acquisition module (16) captures images of the microfluidic chip (3) and its injection ports and transmits them to the image processing module (17). The image processing module (17) preprocesses and detects the target of the images, identifies the positions and characteristics of all injection ports and transmits the characteristic information to the image recognition module (18). The image recognition module (18) confirms the identity of the injection ports recognized by the image processing module (17) and determines the accurate position of the target injection port. The user selects the target injection port through the human-computer interaction module (20). The position feedback and adjustment module (19) feeds back the user's selection to the first servo motor (5) and the second servo motor (9) and starts them, driving the L-shaped plate (13) to move horizontally until the position of the opening groove (14) corresponds to that of the injection port, and then inserting a pipette into the opening groove (14) for guiding and dropping liquid into the microfluidic chip (3).

2. The microfluidic detection device according to claim 1, wherein A first threaded rod (6) and a guide rod (7) are respectively rotatably connected to the inside of the movable frame (4). The first threaded rod (6) is installed at the end of the output shaft of the first servo motor (5). A movable rod (8) is slidably arranged on the outside of the guide rod (7). The movable rod (8) is threadedly connected to the first threaded rod (6).

3. The microfluidic detection device according to claim 2, characterized in that, The bottom of the movable rod (8) is respectively provided with a square groove (15) and a sliding groove (10). The second servo motor (9) is installed inside the square groove (15), and a sliding block (12) is slidably arranged inside the sliding groove (10).

4. The microfluidic detection device according to claim 3, characterized in that, One end of the second servo motor (9) extending to the inside of the sliding groove (10) is provided with a second threaded rod (11). The second threaded rod (11) is threadedly connected with the sliding block (12), and the sliding block (12) is fixedly connected with the L-shaped plate (13).

5. A microfluidic detection device according to claim 4, wherein The size of the opening of the sliding groove (10) is adapted to the size of the sliding block (12), and the cross-section of the sliding groove (10) and the sliding block (12) is convex.

6. The microfluidic detection device according to claim 1, wherein, The image acquisition module (16) includes an image capture unit and a data transmission unit. The image capture unit captures images of the microfluidic chip (3) and its injection ports through a camera and converts them into digital signals. The data transmission unit transmits the captured image data to the image processing module (17) in real time.

7. A microfluidic detection device according to claim 1, wherein, The image processing module (17) includes an image preprocessing unit, a feature extraction unit, and a target detection unit. The image preprocessing unit performs preprocessing operations of denoising and enhancement on the captured images. The feature extraction unit extracts the feature information of the injection ports from the preprocessed images. The target detection unit identifies the positions and features of all injection ports on the microfluidic chip (3).

8. A microfluidic detection device according to claim 1, wherein, The image recognition module (18) includes a feature matching unit and a position accuracy correction unit. The feature matching unit matches the extracted feature information with a preset injection port feature library through a feature matching algorithm to determine the identity of each injection port. The position accuracy correction unit performs accuracy correction on the positions of the matched injection ports; The feature matching algorithm is the Euclidean distance. The Euclidean distance is used to measure the similarity between two feature points. The smaller the Euclidean distance, the more similar the two feature points are; The calculation formula of the Euclidean distance is: Among them, f1 and f2 are the descriptors of two feature points, f 1i and f 2i are the i-th eigenvalue, and n is the dimension of the eigenvector.

9. A microfluidic detection device according to claim 1, wherein, The position feedback and adjustment module (19) includes a path planning unit, a control unit, and a position monitoring unit. The path planning unit calculates the movement path of the L-shaped plate (13) according to the position information of the target injection port through a path planning algorithm. The control unit controls the operation of the first servo motor (5) and the second servo motor (9). The position monitoring unit uses a position sensor to monitor the actual position of the L-shaped plate (13) in real time. The path planning algorithm is the A* algorithm, which is used to find the shortest path from the starting point to the target point in a graphical environment. Its algorithm formula is: Total path cost F(n): F(n) = G(n) + H(n); G(n): The actual cost of reaching the current node n from the starting point; H(n): The estimated cost from the current node n to the target point.

10. A method used by a microfluidic detection device, which employs the microfluidic detection device described in claim 1, characterized in that, It includes the following steps: Step 1: First, pull the movable plate (2) to move the movable plate (2) to the outside of the detection device (1). Then, place the microfluidic chip (3) into the groove of the movable plate (2). Then, rotate the movable frame (4) until the movable frame (4) is parallel to the movable plate (2); Step 2: The image acquisition module (16) captures images of the microfluidic chip (3) and its injection ports, then the image processing module (17) identifies the positions and features of all injection ports. After that, the image recognition module (18) determines the exact positions of the target injection ports. The user selects the target injection port through the human-machine interaction module (20) according to the requirements, and the position feedback and adjustment module (19) feeds back the position information of the injection port selected by the user to the first servo motor (5) and the second servo motor (9). Step 3: After receiving the signals, the first servo motor (5) and the second servo motor (9) start and drive the L-shaped plate (13) to move horizontally until the opening slot (14) corresponds to the position of the injection port. Then, the staff inserts the pipette into the opening slot (14) for guidance and drops the liquid into the microfluidic chip (3). After the liquid dropping is completed, the movable plate (2) is closed, and the detection is carried out by the detection device (1).

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