Stem cell freezer with full-automatic storage function

Abstract

The invention relates to the technical field of stem cell freezers, in particular to a full-automatic storage stem cell freezer which comprises a freezer body, a support is fixed to the inner bottom wall of the freezer body, a conveying belt roller is rotatably mounted on the support through a bearing, a conveying belt is in transmission connection with the conveying belt roller, and a main shaft and an auxiliary shaft are rotatably mounted on the freezer body. The main shaft and the auxiliary shaft are in transmission connection through a first transmission mechanism, the auxiliary shaft and the conveying belt roller are in transmission connection through a second transmission mechanism, a piston cylinder and a liquid nitrogen tank are fixed to the freezer body and communicated through a liquid inlet pipe, a spray head is fixed to the piston cylinder, and the piston cylinder and the spray head are communicated through a liquid outlet pipe. According to the device, the embryonic stem cells can be cooled more uniformly during cryopreservation, meanwhile, liquid nitrogen can be intermittently conveyed into the freezer main body, and the cryopreservation effect of the embryonic stem cells is guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell freezing banks, and in particular to a fully automatic storage stem cell freezing bank. Background Art

[0002] Embryonic stem cells (ES) are cells isolated from early embryos or primordial gonads. They possess the properties of unlimited proliferation, self-renewal, and multidirectional differentiation in vitro. They can be induced to differentiate into nearly all cell types in the body, both in vitro and in vivo. ES cells differ significantly from standard cells, possessing specific growth characteristics and markers. These include very high alkaline phosphatase activity and the presence of embryonic-stage-specific surface antigens. Human ES cells also harbor high-molecular-weight glycoproteins such as TRA1-60 and TRA-1-81, which are markers that allow for the identification of ES cells. Furthermore, ES cells can be permanently passaged in vitro while maintaining a normal karyotype. When cultured in vitro with differentiation inhibitors such as leukemia inhibitory factor or on mouse embryonic fibroblast feeder layers, ES cells can exhibit clonal proliferation, maintaining a normal and stable karyotype for long periods. Their undifferentiated nature is not affected by freezing and thawing.

[0003] When preserving embryonic stem cells, they are usually first placed in a freezer at minus 80 degrees Celsius for storage, and then placed in a preservation box filled with liquid nitrogen for long-term storage. However, the existing freezers on the market often have many different problems when used.

[0004] For example, in existing freezers, liquid nitrogen is delivered from a fixed point to the interior of the freezer for short-term frozen embryonic stem cell storage. This causes uneven distribution of cold within the freezer, resulting in uneven cooling of the embryonic stem cells, affecting their preservation quality. To address this issue, we provide a fully automated stem cell freezer. Summary of the Invention

[0005] The object of the present invention is to provide a fully automatic stem cell cryopreservation bank to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A fully automatic storage stem cell freezing bank comprises a freezing bank main body, a bracket is fixed to the bottom wall of the freezing bank main body, a conveyor belt roller is rotatably installed on the bracket through a bearing, a conveyor belt roller is transmission-connected to a conveyor belt, a main shaft and a secondary shaft are rotatably installed on the freezing bank main body, the main shaft and the secondary shaft are transmission-connected by a first transmission mechanism, the secondary shaft and the conveyor belt roller are transmission-connected by a second transmission mechanism, a piston cylinder and a liquid nitrogen tank are fixed on the freezing bank main body, the piston cylinder and the liquid nitrogen tank are connected by a liquid inlet pipe, a nozzle is fixed on the piston cylinder, the piston cylinder and the nozzle are connected by a liquid outlet pipe, a rotating rod is rotatably installed on the piston cylinder, the rotating rod and the main shaft are transmission-connected by a third transmission mechanism, a piston is movably clamped in the piston cylinder, the rotating rod and the piston are transmission-connected by a fourth transmission mechanism, and the rotating rod drives the piston to move back and forth horizontally when it rotates.

[0008] In the fully automatic stem cell freezing bank as described above, an inspection door is hingedly mounted on the front of the freezing bank body.

[0009] In the fully automatic stem cell freezing bank as described above, a motor is fixedly mounted on the freezing bank body, and the main shaft is fixedly mounted on the output end of the motor.

[0010] In the fully automatic stem cell freezing bank as described above, the first transmission mechanism includes a worm fixed on the main shaft and a worm wheel fixed on the secondary shaft, and the worm is cooperatively connected with the worm wheel.

[0011] In the fully automatic stem cell cryopreservation bank described above, the second transmission mechanism includes a first driving pulley fixed on the secondary shaft and a first driven pulley fixed on the conveyor belt roller, and the first driving pulley and the first driven pulley are connected via a first belt transmission.

[0012] In the fully automatic stem cell freezing bank as described above, one-way valves are respectively installed on the liquid inlet pipe and the liquid outlet pipe.

[0013] In the fully automatic stem cell cryopreservation bank described above, the third transmission mechanism includes a second driving pulley fixed on the main shaft and a second driven pulley fixed on the rotating rod, and the second driving pulley and the second driven pulley are connected via a second belt transmission.

[0014] In the fully automatic stem cell cryopreservation bank as described above, the fourth transmission mechanism includes a movable rod fixed on the rotating rod, a connecting rod is provided between the movable rod and the piston, and both ends of the connecting rod are hinged to the movable rod and the connecting rod respectively.

[0015] Compared with the prior art, the present invention has the following advantages: when embryonic stem cells are frozen for short-term storage, the embryonic stem cells are first placed in a container and placed on a conveyor belt. The motor is started to drive the main shaft to rotate forward and reverse, which drives the secondary shaft to rotate, and then drives the conveyor roller to rotate, thereby driving the conveyor belt to rotate. After the container storing the embryonic stem cells is placed on the conveyor belt, it can move back and forth within the freezer body to change its position. In addition, the rotation of the main shaft drives the rotating rod to rotate, which drives the piston to move back and forth within the piston cylinder, thereby continuously drawing liquid nitrogen from the liquid nitrogen tank into the piston cylinder and pushing it to the nozzle for spraying. In conjunction with the back and forth movement of the embryonic stem cells on the conveyor belt below the nozzle, the cooling position of the embryonic stem cells can be continuously changed, thereby avoiding the situation where the liquid nitrogen is supplied from a fixed point, resulting in uneven cooling of the embryonic stem cells and affecting the preservation quality.

[0016] Therefore, the present invention can make the embryonic stem cells cooled more evenly during cryopreservation, and can intermittently transport liquid nitrogen into the interior of the freezer body, thereby ensuring the cryopreservation effect of the embryonic stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of the overall structure of a fully automatic storage stem cell cryopreservation bank.

[0018] Figure 2 Schematic diagram of the overall structure of a fully automatic storage stem cell cryopreservation bank.

[0019] Figure 3 This is a schematic diagram of the structure of the first transmission mechanism of a fully automatic storage stem cell freezing bank.

[0020] Figure 4 This is a schematic diagram of the conveyor rollers and conveyor belt structure of a fully automatic storage stem cell freezing bank.

[0021] Figure 5 A fully automated stem cell cryopreservation system Figure 2 Schematic diagram of the partially enlarged structure.

[0022] In the figure: 1. Freezer body; 2. Bracket; 3. Conveyor roller; 4. Conveyor belt; 5. Motor; 6. Main shaft; 7. Countershaft; 8. Worm; 9. Worm gear; 10. First driving pulley; 11. First driven pulley; 12. First belt; 13. Piston cylinder; 14. Liquid nitrogen tank; 15. Liquid inlet pipe; 16. Rotating rod; 17. Second driving pulley; 18. Second driven pulley; 19. Second belt; 20. Piston; 21. Movable rod; 22. Connecting rod; 23. Liquid outlet pipe; 24. Nozzle. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0024] See also Figures 1 to 5 As an embodiment of the present invention, a fully automatic storage stem cell freezing bank includes a freezing bank body 1, a bracket 2 is fixed to the bottom wall of the freezing bank body 1, a conveyor roller 3 is rotatably mounted on the bracket 2 through a bearing, a conveyor belt 4 is connected to the conveyor belt roller 3, a main shaft 6 and a secondary shaft 7 are rotatably mounted on the freezing bank body 1, the main shaft 6 and the secondary shaft 7 are connected by a first transmission mechanism, and the secondary shaft 7 and the conveyor belt roller 3 are connected by a second transmission mechanism. A piston cylinder 1 is fixed on the freezing bank body 1. 3 and the liquid nitrogen tank 14, the piston cylinder 13 and the liquid nitrogen tank 14 are connected through a liquid inlet pipe 15, a nozzle 24 is fixed on the piston cylinder 13, and the piston cylinder 13 and the nozzle 24 are connected through a liquid outlet pipe 23, a rotating rod 16 is rotatably mounted on the piston cylinder 13, and the rotating rod 16 and the main shaft 6 are connected by a third transmission mechanism, a piston 20 is movably clamped in the piston cylinder 13, and the rotating rod 16 and the piston 20 are connected by a fourth transmission mechanism, and the rotating rod 16 drives the piston 20 to move back and forth horizontally when it rotates.

[0025] In this embodiment, when embryonic stem cells are frozen for short-term storage, the embryonic stem cells are first placed in a container and placed on the conveyor belt 4. The motor 5 is started to drive the main shaft 6 to rotate forward and reverse, which drives the secondary shaft 7 to rotate, and then drives the conveyor roller 3 to rotate, thereby driving the conveyor belt 4 to rotate. Then, after the container storing the embryonic stem cells is placed on the conveyor belt 4, it can move back and forth within the freezer body 1 to change its position. In addition, the rotation of the main shaft 6 drives the rotating rod 16 to rotate, thereby driving the piston 20 to move back and forth within the piston cylinder 13, thereby continuously drawing liquid nitrogen from the liquid nitrogen tank 14 into the interior of the piston cylinder 13 and pushing it to the nozzle 24 for spraying. In conjunction with the back and forth movement of the embryonic stem cells on the conveyor belt 4 below the nozzle 24, the cooling position of the embryonic stem cells can be continuously changed.

[0026] As a further solution of the present invention, an inspection door is hingedly installed on the front of the freezer body 1.

[0027] In this embodiment, it is convenient to perform later inspection and maintenance on the interior of the freezer body 1 .

[0028] As a further solution of the present invention, a motor 5 is fixedly mounted on the freezer body 1 , and a main shaft 6 is fixedly mounted on an output end of the motor 5 .

[0029] In this embodiment, the starting motor 5 can drive the main shaft 6 to rotate clockwise or counterclockwise, thereby driving the conveyor belt 4 to rotate clockwise or counterclockwise, thereby driving the embryonic stem cells on the conveyor belt 4 to move back and forth and change their positions.

[0030] As a further solution of the present invention, the first transmission mechanism includes a worm 8 fixed on the main shaft 6 and a worm wheel 9 fixed on the secondary shaft 7, and the worm 8 and the worm wheel 9 are cooperatively connected.

[0031] In this embodiment, when the main shaft 6 rotates, it drives the worm 8 to rotate. The worm 8 is connected with the worm wheel 9 to drive the worm wheel 9 to rotate, thereby driving the secondary shaft 7 to rotate.

[0032] As a further solution of the present invention, the second transmission mechanism includes a first driving pulley 10 fixed on the secondary shaft 7 and a first driven pulley 11 fixed on the conveyor belt roller 3. The first driving pulley 10 and the first driven pulley 11 are connected by a first belt 12.

[0033] In this embodiment, when the secondary shaft 7 rotates, it drives the first driving pulley 10 to rotate. The first driving pulley 10 and the first driven pulley 11 are connected through the first belt 12 to drive the first driven pulley 11 to rotate, thereby driving the conveyor belt roller 3 to rotate.

[0034] As a further solution of the present invention, one-way valves are respectively installed on the liquid inlet pipe 15 and the liquid outlet pipe 23.

[0035] In this embodiment, it is ensured that the liquid nitrogen in the liquid nitrogen tank 14 is unidirectionally extracted into the interior of the piston cylinder 13 and unidirectionally transported to the interior of the nozzle 24 through the liquid outlet pipe 23 .

[0036] As a further solution of the present invention, the third transmission mechanism includes a second driving pulley 17 fixed on the main shaft 6 and a second driven pulley 18 fixed on the rotating rod 16. The second driving pulley 17 and the second driven pulley 18 are connected by a second belt 19.

[0037] In this embodiment, when the main shaft 6 rotates, it will drive the second driving pulley 17 to rotate. The second driving pulley 17 and the second driven pulley 18 are connected through the second belt 19 to drive the second driven pulley 18 to rotate, thereby driving the rotating rod 16 to rotate.

[0038] As a further solution of the present invention, the fourth transmission mechanism includes a movable rod 21 fixed on the rotating rod 16, a connecting rod 22 is provided between the movable rod 21 and the piston 20, and both ends of the connecting rod 22 are hinged to the movable rod 21 and the connecting rod 22 respectively.

[0039] In this embodiment, when the rotating rod 16 rotates, it drives the movable rod 21 to rotate. When the movable rod 21 rotates, it drives the connecting rod 22 to swing, thereby driving the piston 20 to move back and forth inside the piston cylinder 13.

[0040] The working principle of the present invention is as follows: when embryonic stem cells are frozen for short-term storage, the embryonic stem cells are first placed in a container and placed on the conveyor belt 4. The motor 5 is started to drive the main shaft 6 to rotate forward and reverse, which can drive the secondary shaft 7 to rotate and then drive the conveyor roller 3 to rotate, thereby driving the conveyor belt 4 to rotate. Then, after the container storing the embryonic stem cells is placed on the conveyor belt 4, it can move back and forth inside the freezer body 1 to change its position. In addition, when the main shaft 6 rotates, it drives the rotating rod 16 to rotate, thereby driving the piston 20 to move back and forth inside the piston cylinder 13, thereby continuously extracting liquid nitrogen from the liquid nitrogen tank 14 into the piston cylinder 13 and pushing it to the nozzle 24 for spraying. In conjunction with the back and forth movement of the embryonic stem cells on the conveyor belt 4 below the nozzle 24, the cooling position of the embryonic stem cells can be continuously changed, thereby avoiding the situation where the liquid nitrogen is supplied from a fixed point, resulting in uneven cooling of the embryonic stem cells and affecting the preservation quality.

[0041] The above embodiments are exemplary rather than restrictive, so any technical solution that can be implemented in other specific forms without departing from the spirit or basic features of the present invention is included in the present invention.

Claims

1. A fully automatic storage stem cell cryopreservation bank, comprising a cryopreservation body (1), characterized in that: A bracket (2) is fixed to the bottom wall of the interior of the freezer main body (1), a conveyor roller (3) is rotatably mounted on the bracket (2) via a bearing, a conveyor belt (4) is transmission-connected to the conveyor belt roller (3), a main shaft (6) and a secondary shaft (7) are rotatably mounted on the freezer main body (1), the main shaft (6) and the secondary shaft (7) are transmission-connected via a first transmission mechanism, the secondary shaft (7) and the conveyor belt roller (3) are transmission-connected via a second transmission mechanism, a piston cylinder (13) and a liquid nitrogen tank (14) are fixed to the freezer main body (1), the piston cylinder (13) and the liquid nitrogen tank are transmission-connected to each other. (14) are connected via a liquid inlet pipe (15), a nozzle (24) is fixed on the piston cylinder (13), and the piston cylinder (13) and the nozzle (24) are connected via a liquid outlet pipe (23). A rotating rod (16) is rotatably mounted on the piston cylinder (13), and the rotating rod (16) and the main shaft (6) are connected via a third transmission mechanism. A piston (20) is movably engaged in the piston cylinder (13), and the rotating rod (16) and the piston (20) are connected via a fourth transmission mechanism. When the rotating rod (16) rotates, it drives the piston (20) to move back and forth horizontally.

2. A fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: An inspection door is hingedly mounted on the front of the freezer main body (1) via a hinge.

3. The fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: A motor (5) is fixedly mounted on the freezer main body (1), and the main shaft (6) is fixedly mounted on the output end of the motor (5).

4. The fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: The first transmission mechanism comprises a worm (8) fixed on the main shaft (6) and a worm wheel (9) fixed on the secondary shaft (7), and the worm (8) and the worm wheel (9) are cooperatively connected.

5. The fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: The second transmission mechanism comprises a first driving pulley (10) fixed on the secondary shaft (7) and a first driven pulley (11) fixed on the conveyor belt roller (3); the first driving pulley (10) and the first driven pulley (11) are connected to each other through a first belt (12).

6. The fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: One-way valves are respectively installed on the liquid inlet pipe (15) and the liquid outlet pipe (23).

7. The fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: The third transmission mechanism comprises a second driving pulley (17) fixed on the main shaft (6) and a second driven pulley (18) fixed on the rotating rod (16); the second driving pulley (17) and the second driven pulley (18) are connected to each other through a second belt (19).

8. The fully automatic stem cell cryopreservation bank according to claim 1, characterized in that: The fourth transmission mechanism comprises a movable rod (21) fixed on the rotating rod (16), a connecting rod (22) is provided between the movable rod (21) and the piston (20), and two ends of the connecting rod (22) are respectively hinged to the movable rod (21) and the connecting rod (22).

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