Refreshing and anti-fatigue rhizoma polygonati and coffee compound beverage and preparation process thereof

Abstract

The invention relates to the technical field of beverage preparation, in particular to a refreshing and anti-fatigue rhizoma polygonati and coffee compound beverage and a preparation technology thereof. The preparation process comprises the following steps: preparing polygonatum polysaccharide; preparing gardenia pectic polysaccharide; preparing rhizoma polygonati extract gel microspheres; the refreshing and anti-fatigue rhizoma polygonati and coffee compound beverage is prepared. According to the invention, the gardenia pectic polysaccharides and the lecithin are combined and then used for coating the polygonatum saponin, and after the gardenia pectic polysaccharides and the lecithin are compounded, the substances coated in the gel can be effectively prevented from being gathered, precipitated or separated, the coated active substances can be better protected, and the influence of external environmental factors on the chemical properties of the gel is reduced; the sealwort coffee is kept in a stable state in the storage and transportation processes, and the taste enjoying period and the quality guarantee period of the sealwort coffee are prolonged.

Description

Technical Field

[0001] The invention relates to the technical field of beverage preparation, and in particular to a refreshing and anti-fatigue polygonatum coffee compound beverage and a preparation process thereof. Background Art

[0002] Polygonatum sibiricum, the dried rhizome of Polygonatum jingianum (Coll. et Hemsl.), a plant of the Liliaceae family, is a traditional Chinese medicinal herb used both as a medicine and a food. It is sweet, neutral, and non-toxic, and enters the lung, spleen, and kidney meridians. Polygonatum sibiricum contains a variety of active ingredients, such as polygonatum polysaccharides, saponins, and flavonoids, which have the effects of tonifying qi and strengthening the spleen, moistening the lungs and nourishing yin, and nourishing the kidneys and replenishing essence. In 2002, it was included in the "List of Items that are Both Food and Medicine," making it a dual-use food resource with diverse functional ingredients. It contains numerous beneficial active ingredients, such as polygonatum polysaccharides, saponins, and flavonoids, which have the potential to regulate blood sugar and lipids, protect the cardiovascular system, and provide mental clarity, fatigue relief, and antioxidant benefits. Therefore, Polygonatum sibiricum can be incorporated into beverages as a functional food, enhancing their health benefits.

[0003] Polygonatum sibiricum saponin has good antioxidant capacity, can scavenge free radicals in the body, reduce oxidative stress damage, and thus delay cell aging. However, the chemical properties of polygonatum sibiricum saponin are relatively unstable and are easily affected by external environmental factors, such as temperature, light, pH, etc. Directly adding polygonatum sibiricum saponin is very challenging for the subsequent storage and transportation of the beverage, and the stability of polygonatum sibiricum saponin cannot be guaranteed. Moreover, polygonatum sibiricum saponin itself contains bitterness, and direct addition will affect the flavor of the beverage. Therefore, the present invention coats polygonatum sibiricum saponin and makes it into gel microbeads. The coating provides a protective barrier for polygonatum, reduces the unstable factors that may occur in the storage and transportation links, and increases the shelf life of polygonatum sibiricum saponin. Moreover, the bitterness of these polygonatum sibiricum saponins can be concealed by this coating shell, so that the taste and flavor of the beverage are more in line with consumer preferences. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the present invention aims to provide a refreshing and anti-fatigue polygonatum coffee compound beverage and a preparation process thereof.

[0005] A preparation process of a refreshing and anti-fatigue polygonatum coffee compound beverage comprises the following steps:

[0006] S1: Preparation of Polygonatum sibiricum Polysaccharide

[0007] S1.1: Weigh 5-6 parts by weight of dried Polygonatum sibiricum slices, add 10-15 parts by weight of distilled water as the extraction solvent, beat in a pulper for 5-10 minutes, remove from the pulp, and high-speed shear at a speed of 10,000-12,000 rpm for 5-10 minutes. Then, add 1-5% of the system weight of a biological enzyme, extract at a pH of 6-7 and a temperature of 50-60°C for 20-30 minutes, then inactivate the enzyme in a boiling water bath for 10-15 minutes, cool to 0-4°C, and centrifuge at 5,000-5,500 rpm for 10-15 minutes to obtain a supernatant. Concentrate the supernatant to one-fifth of the original volume to obtain a Polygonatum sibiricum polysaccharide extract;

[0008] S1.2: The polysaccharide extract of polygonatum sibiricum was added to petroleum ether-acetone solvent at a material-liquid ratio of 1g: (4-5)mL and refluxed for degreasing twice, each time for 2-2.5h. After the solvent was evaporated, anhydrous ethanol 4 times the volume of the system was added and allowed to stand at 0-4℃ for 12-13h. The precipitate was collected after centrifugation and dissolved in an equal volume of distilled water. Then, 30% of the volume of the system Sevage reagent was added for deproteinization. The supernatant was collected and the above operation was repeated until there was no obvious protein between the polysaccharide phase and the lower chloroform-n-butanol phase. The defatted and deproteinized solution was dialyzed, the polysaccharide solution was collected and freeze-dried to obtain polygonatum sibiricum polysaccharide.

[0009] Furthermore, the biological enzyme is one or more combinations of papain, cellulase, and hemicellulase.

[0010] Furthermore, the volume ratio of petroleum ether to acetone in the petroleum ether-acetone solvent is 1:1.

[0011] S2: Preparation of Gardenia jasminoides pectin polysaccharide

[0012] S2.1: Dry fresh gardenia flowers in the sun, grind them into powder using a high-speed multifunctional grinder, and pass them through a 40-mesh sieve for later use. Take 10-12 parts by weight of pollen and stir them with 100-110 parts by weight of 85% ethanol using an electric stirrer overnight. Filter the mixture, soak the residue again in anhydrous ethanol overnight, filter it, and then dry it to obtain the gardenia pollen.

[0013] S2.2: Accurately weigh 3-5 parts by mass of gardenia pollen, add 120-130 parts by mass of distilled water, and extract for 2-3 hours at a temperature of 60-100° C. to obtain gardenia pectin polysaccharide.

[0014] S3: Preparation of Polygonatum sibiricum Extract Gel Microspheres

[0015] S3.1: Grind dried polygonatum slices and pass them through an 80-mesh sieve to obtain polygonatum powder. Soak the polygonatum powder in 60-70 wt% ethanol for 12-13 hours at a solid-liquid ratio of 1 g polygonatum powder to ethanol of (10-12) mL. Centrifuge for 10-15 minutes, collect the supernatant, and evaporate the ethanol solvent to obtain a polygonatum saponin extract. Mix and dissolve the polygonatum saponin extract with distilled water at a solid-liquid ratio of 1 g to (20-25) mL to obtain a polygonatum saponin extract solution.

[0016] S3.2: To 10-12 parts by volume of a 4-5 wt% solution of Gardenia jasminoides pectin polysaccharide, add 0.5 mol / L NaOH solution and reduce the ester content for 30-35 minutes. Then, add 15-18 parts by volume of 4-8 wt% lecithin and disperse it. Then, add 10-15 parts by volume of a polygonatum saponin extract and sonicate until a uniform suspension is obtained.

[0017] S3.3: The pH value of the calcium chloride solution is then adjusted to 2-3 with 1 mol / L HCl, and 100 parts by volume of the calcium chloride solution is dripped into the suspension through a syringe to form gel balls. The mixture is stirred for 50-60 minutes for cross-linking reaction, filtered, washed, and dried at 50-60°C for 24-25 hours to obtain polygonatum extract gel microspheres.

[0018] Furthermore, the concentration of the calcium chloride solution is 4-5 wt %.

[0019] Furthermore, the syringe needle has a diameter of 0.8 mm and a needle height of 2 cm.

[0020] S4: Preparation of refreshing and anti-fatigue Huangjing coffee compound beverage

[0021] S4.1: Mix polygonatum polysaccharide with distilled water at a material-liquid ratio of 1 g:(5-6) mL to obtain a polygonatum polysaccharide soak solution. Soak polygonatum extract gel microspheres in the polygonatum polysaccharide soak solution for 1-2 hours, then freeze-dry at -15°C for 3-4 hours, crush, and pass through an 80-mesh sieve to obtain a polygonatum additive.

[0022] S4.2: Mix 20-25 parts by weight of coffee, 2-5 parts by weight of polygonatum additive, and 2-8 parts by weight of non-dairy creamer, and stir evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage.

[0023] A refreshing and anti-fatigue polygonatum coffee compound beverage is prepared by the above-mentioned refreshing and anti-fatigue polygonatum coffee compound beverage preparation process.

[0024] Compared with the prior art, the present invention has at least the following beneficial effects:

[0025] 1. The present invention combines gardenia pectin polysaccharide and lecithin to coat polygonatum saponin. Lecithin has good emulsification and dispersibility, and can make the components in the gel system evenly distributed. Gardenia pectin polysaccharide can form a three-dimensional network structure, enhancing the physical stability of the gel. After the two are combined, this synergistic effect can effectively prevent the substances wrapped in the gel from aggregating, precipitating or separating. In addition, the chemical structure of gardenia pectin polysaccharide makes it relatively stable under different pH values and temperature conditions. Lecithin also has a certain chemical stability and is not easily oxidized and decomposed. When they are used in combination, they can better Protect the encapsulated active substances, reduce the impact of external environmental factors on their chemical properties, keep them in a stable state during storage and transportation, and extend the appreciation period and shelf life of Polygonatum coffee. At the same time, Gardenia pectin polysaccharide can slow down the release rate of active ingredients, and the liposome structure formed by lecithin can also play a sustained release role. After the two are compounded, the release rate can be controlled by adjusting the ratio of pectin polysaccharide and lecithin and the preparation conditions of the gel, so that the encapsulated substances can be slowly released in the expected manner and speed, thereby increasing the flavor retention time in food and improving the antioxidant capacity brought by Polygonatum coffee.

[0026] 2. The present invention soaks the polygonatum extract gel microspheres in polygonatum polysaccharide. The polygonatum extract gel microspheres form a stable and smooth inclusion surface through the inclusion ability of lecithin, and polygonatum polysaccharide can increase the viscosity and water retention of food. By soaking the polygonatum extract gel microspheres in polygonatum polysaccharide, the taste and texture of the polygonatum extract gel microspheres can be improved, making them more delicate and soft. In addition, polygonatum polysaccharide will contain a small amount of sweet monosaccharides and glucose during the preparation process, so that the polygonatum polysaccharide has a certain sweetness and reduces the bitterness of polygonatum coffee. At the same time, polygonatum polysaccharide also has an antioxidant effect. The gel microspheres soaked in polygonatum polysaccharide can better protect the active ingredients in the food and prevent its oxidation and degradation, thereby extending the shelf life of the food, improving the stability of the food, and bringing considerable antioxidant capacity to the polygonatum coffee. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and to enable one skilled in the relevant art to make and use the present disclosure.

[0028] Figure 1 This is a flow chart of the preparation process of a refreshing and anti-fatigue polygonatum coffee compound beverage adopted in an embodiment of the present invention. DETAILED DESCRIPTION

[0029] The following, combined with the accompanying drawings and specific examples, describes in detail the refreshing and anti-fatigue Polygonatum coffee compound beverage and its preparation process provided by the present invention. It is also noted that, for the sake of completeness, the following examples are best and preferred embodiments, and those skilled in the art may employ alternative implementations for known techniques. Furthermore, the accompanying drawings are intended only to provide a more detailed description of the embodiments and are not intended to limit the present invention.

[0030] Example 1:

[0031] A preparation process of a refreshing and anti-fatigue polygonatum coffee compound beverage, such as Figure 1 As shown, the following steps are included:

[0032] S1: Preparation of Polygonatum sibiricum Polysaccharide

[0033] S1.1: Weigh 5 parts by weight of dried Polygonatum sibiricum slices, add 10 parts by weight of distilled water as the extraction solvent, beat in a pulper for 5 minutes, remove from the pulp, and high-speed shear at 10,000 rpm for 5 minutes. Then, add 1% by weight of papain, extract at pH 6 and 50°C for 20 minutes, then inactivate the enzyme in a boiling water bath for 10 minutes, cool to 0°C, and centrifuge at 5,000 rpm for 10 minutes to obtain a supernatant. Concentrate the supernatant to one-fifth of its original volume to obtain a Polygonatum sibiricum polysaccharide extract;

[0034] S1.2: The Polygonatum sibiricum polysaccharide extract was added to petroleum ether-acetone solvent at a material-liquid ratio of 1g:4mL, and refluxed for degreasing twice. The volume ratio of petroleum ether and acetone in the petroleum ether-acetone solvent was 1:1, and each time was 2h. After evaporating the solvent, anhydrous ethanol 4 times the volume of the system was added, and the mixture was allowed to stand at 0℃ for 12h. After centrifugation, the precipitate was collected and dissolved in an equal volume of distilled water. Then, 30% of the volume of the system Sevage reagent was added for deproteinization. The supernatant was collected and the above operation was repeated until there was no obvious protein between the polysaccharide phase and the lower chloroform-n-butanol phase. The defatted and deproteinized solution was dialyzed, the polysaccharide solution was collected and freeze-dried to obtain Polygonatum sibiricum polysaccharide.

[0035] S2: Preparation of Gardenia jasminoides pectin polysaccharide

[0036] S2.1: Dry fresh gardenia flowers in the sun, grind them into powder using a high-speed multifunctional grinder, and pass them through a 40-mesh sieve for later use. Take 10 parts by weight of pollen and stir it overnight with 100 parts by weight of 85% ethanol using an electric stirrer. Filter the mixture, soak the residue again in anhydrous ethanol overnight, filter it again, and dry it to obtain the gardenia pollen.

[0037] S2.2: Accurately weigh 3 parts by mass of gardenia pollen, add 120 parts by mass of distilled water, and soak for 2 hours at a soaking temperature of 60° C. to obtain gardenia pectin polysaccharide.

[0038] S3: Preparation of Polygonatum sibiricum Extract Gel Microspheres

[0039] S3.1: Grind dried polygonatum slices and pass them through an 80-mesh sieve to obtain polygonatum powder. Soak the polygonatum powder in 60 wt% ethanol for 12 hours at a solid-liquid ratio of 1 g polygonatum powder to 10 mL ethanol. Centrifuge for 10 minutes, collect the supernatant, and evaporate the ethanol solvent to obtain a polygonatum saponin extract. Mix and dissolve the polygonatum saponin extract with distilled water at a solid-liquid ratio of 1 g to 20 mL to obtain a polygonatum saponin extract solution.

[0040] S3.2: To 10 parts by volume of a 4 wt% solution of Gardenia jasminoides pectin polysaccharide, 0.5 mol / L NaOH solution was added for 30 minutes to reduce the ester content. Then, 15 parts by volume of 4 wt% lecithin was added and dispersed. Then, 10 parts by volume of a polygonatum saponin extract was added and sonicated until a homogeneous suspension was obtained.

[0041] S3.3: The pH value of the 4wt% calcium chloride solution was adjusted to 2 with 1mol / L HCl, and 100 parts by volume of the calcium chloride solution was dripped into the suspension through a syringe with a needle diameter of 0.8mm and a needle height of 2cm to form gel balls. The mixture was stirred for 50min to carry out a cross-linking reaction, filtered, washed, and dried at 50°C for 24h to obtain polygonatum extract gel microspheres.

[0042] S4: Preparation of refreshing and anti-fatigue Huangjing coffee compound beverage

[0043] S4.1: Mix polygonatum polysaccharide with distilled water at a material-liquid ratio of 1 g:5 mL to obtain a polygonatum polysaccharide soaking solution. Soak polygonatum extract gel microspheres in the polygonatum polysaccharide soaking solution for 1 hour, then freeze-dry at -15°C for 3 hours, crush, and pass through an 80-mesh sieve to obtain a polygonatum additive.

[0044] S4.2: Mix 20 parts by mass of coffee, 2 parts by mass of polygonatum additive, and 2 parts by mass of non-dairy creamer, and stir evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage.

[0045] Example 2:

[0046] A preparation process of a refreshing and anti-fatigue polygonatum coffee compound beverage, such as Figure 1 As shown, the following steps are included:

[0047] S1: Preparation of Polygonatum sibiricum Polysaccharide

[0048] S1.1: Weigh 6 parts by weight of dried Polygonatum sibiricum slices, add 15 parts by weight of distilled water as the extraction solvent, beat in a pulper for 5 minutes, remove from the pulp, and high-speed shear at 10,000 rpm for 5 minutes. Then, add 1% by weight of papain, extract at pH 6 and 50°C for 20 minutes, then inactivate the enzyme in a boiling water bath for 10 minutes, cool to 0°C, and centrifuge at 5,000 rpm for 10 minutes to obtain a supernatant. Concentrate the supernatant to one-fifth of its original volume to obtain a Polygonatum sibiricum polysaccharide extract;

[0049] S1.2: The Polygonatum sibiricum polysaccharide extract was added to petroleum ether-acetone solvent at a material-liquid ratio of 1g:5mL, and refluxed for degreasing twice. The volume ratio of petroleum ether and acetone in the petroleum ether-acetone solvent was 1:1, and each time was 2 hours. After evaporating the solvent, anhydrous ethanol 4 times the volume of the system was added, and the mixture was allowed to stand at 0℃ for 12 hours. After centrifugation, the precipitate was collected and dissolved in an equal volume of distilled water. Then, 30% of the volume of the system Sevage reagent was added for deproteinization. The supernatant was collected and the above operation was repeated until there was no obvious protein between the polysaccharide phase and the lower chloroform-n-butanol phase. The defatted and deproteinized solution was dialyzed, the polysaccharide solution was collected and freeze-dried to obtain Polygonatum sibiricum polysaccharide.

[0050] S2: Preparation of Gardenia jasminoides pectin polysaccharide

[0051] S2.1: Dry fresh gardenia flowers in the sun, grind them into powder using a high-speed multifunctional grinder, and pass them through a 40-mesh sieve for later use. Take 12 parts by weight of pollen and stir it overnight with 110 parts by weight of 85% ethanol using an electric stirrer. Filter the mixture, soak the residue again in anhydrous ethanol overnight, filter it again, and dry it to obtain the gardenia pollen.

[0052] S2.2: Accurately weigh 5 parts by mass of gardenia pollen, add 130 parts by mass of distilled water, and soak for 2 hours at a soaking temperature of 60° C. to obtain gardenia pectin polysaccharide.

[0053] S3: Preparation of Polygonatum sibiricum Extract Gel Microspheres

[0054] S3.1: Grind dried polygonatum slices and pass them through an 80-mesh sieve to obtain polygonatum powder. Soak the polygonatum powder in 60 wt% ethanol for 12 hours at a solid-liquid ratio of 1 g polygonatum powder to ethanol of 12 mL. Centrifuge for 10 minutes, collect the supernatant, and evaporate the ethanol solvent to obtain a polygonatum saponin extract. Mix and dissolve the polygonatum saponin extract with distilled water at a solid-liquid ratio of 1 g to 25 mL to obtain a polygonatum saponin extract solution.

[0055] S3.2: To 12 parts by volume of a 4 wt% solution of Gardenia jasminoides pectin polysaccharide, 0.5 mol / L NaOH solution was added for 30 minutes to reduce the ester content. Then, 18 parts by volume of 4 wt% lecithin was added and dispersed. Then, 15 parts by volume of a polygonatum saponin extract was added and sonicated until a homogeneous suspension was obtained.

[0056] S3.3: The pH value of the 4wt% calcium chloride solution was adjusted to 2 with 1mol / L HCl, and 100 parts by volume of the calcium chloride solution was dripped into the suspension through a syringe with a needle diameter of 0.8mm and a needle height of 2cm to form gel balls. The mixture was stirred for 50min to carry out a cross-linking reaction, filtered, washed, and dried at 50°C for 24h to obtain polygonatum extract gel microspheres.

[0057] S4: Preparation of refreshing and anti-fatigue Huangjing coffee compound beverage

[0058] S4.1: Mix polygonatum polysaccharide with distilled water at a material-liquid ratio of 1 g:6 mL to obtain a polygonatum polysaccharide soaking solution. Soak polygonatum extract gel microspheres in the polygonatum polysaccharide soaking solution for 1 hour, then freeze-dry at -15°C for 3 hours, crush, and pass through an 80-mesh sieve to obtain a polygonatum additive.

[0059] S4.2: Mix 25 parts by weight of coffee, 5 parts by weight of polygonatum additive, and 8 parts by weight of non-dairy creamer, and stir evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage.

[0060] Example 3:

[0061] A preparation process of a refreshing and anti-fatigue polygonatum coffee compound beverage, such as Figure 1 As shown, the following steps are included:

[0062] S1: Preparation of Polygonatum sibiricum Polysaccharide

[0063] S1.1: Weigh 5 parts by weight of dried Polygonatum sibiricum slices, add 10 parts by weight of distilled water as the extraction solvent, beat in a pulper for 10 minutes, remove from the pulp, and high-speed shear at 12,000 rpm for 10 minutes. Then, add 5% by weight of papain, extract at pH 7 and 60°C for 30 minutes, then inactivate the enzyme in a boiling water bath for 15 minutes, cool to 4°C, and centrifuge at 5,500 rpm for 15 minutes to obtain a supernatant. Concentrate the supernatant to one-fifth of its original volume to obtain a Polygonatum sibiricum polysaccharide extract;

[0064] S1.2: The polysaccharide extract of polygonatum sibiricum was added to petroleum ether-acetone solvent at a material-liquid ratio of 1g:4mL, and the mixture was refluxed for degreasing twice. The volume ratio of petroleum ether and acetone in the petroleum ether-acetone solvent was 1:1, and the reaction was repeated for 2.5 hours each time. After the solvent was evaporated, anhydrous ethanol 4 times the volume of the system was added, and the mixture was allowed to stand at 0℃ for 13 hours. After centrifugation, the precipitate was collected and dissolved in an equal volume of distilled water. Then, 30% of the volume of the system Sevage reagent was added for deproteinization. The supernatant was collected and the above operation was repeated until there was no obvious protein between the polysaccharide phase and the lower chloroform-n-butanol phase. The defatted and deproteinized solution was dialyzed, the polysaccharide solution was collected and freeze-dried to obtain polygonatum sibiricum polysaccharide.

[0065] S2: Preparation of Gardenia jasminoides pectin polysaccharide

[0066] S2.1: Dry fresh gardenia flowers in the sun, grind them into powder using a high-speed multifunctional grinder, and pass them through a 40-mesh sieve for later use. Take 10 parts by weight of pollen and stir it overnight with 100 parts by weight of 85% ethanol using an electric stirrer. Filter the mixture, soak the residue again in anhydrous ethanol overnight, filter it again, and dry it to obtain the gardenia pollen.

[0067] S2.2: Accurately weigh 3 parts by mass of gardenia pollen, add 120 parts by mass of distilled water, and soak for 3 hours at 80° C. to obtain gardenia pectin polysaccharide.

[0068] S3: Preparation of Polygonatum sibiricum Extract Gel Microspheres

[0069] S3.1: Grind dried polygonatum slices and pass them through an 80-mesh sieve to obtain polygonatum powder. Soak the polygonatum powder in 70 wt% ethanol for 13 hours at a solid-liquid ratio of 1 g polygonatum powder to 10 mL ethanol. Centrifuge for 15 minutes, collect the supernatant, and evaporate the ethanol solvent to obtain a polygonatum saponin extract. Mix and dissolve the polygonatum saponin extract with distilled water at a solid-liquid ratio of 1 g to 20 mL to obtain a polygonatum saponin extract solution.

[0070] S3.2: To 10 parts by volume of a 4 wt% solution of Gardenia jasminoides pectin polysaccharide, 0.5 mol / L NaOH solution was added for 35 minutes to reduce the ester content. Then, 15 parts by volume of 4 wt% lecithin was added and dispersed. Then, 10 parts by volume of a polygonatum saponin extract was added and sonicated until a homogeneous suspension was obtained.

[0071] S3.3: The pH value of the 4wt% calcium chloride solution was adjusted to 3 with 1mol / L HCl, and 100 parts by volume of the calcium chloride solution was dripped into the suspension through a syringe with a needle diameter of 0.8mm and a needle height of 2cm. The mixture was stirred for 50min to carry out a cross-linking reaction, filtered, washed, and dried at 60°C for 25h to obtain polygonatum extract gel microspheres.

[0072] S4: Preparation of refreshing and anti-fatigue Huangjing coffee compound beverage

[0073] S4.1: Mix polygonatum polysaccharide with distilled water at a material-liquid ratio of 1 g:5 mL to obtain a polygonatum polysaccharide soaking solution. Soak polygonatum extract gel microspheres in the polygonatum polysaccharide soaking solution for 2 hours, then freeze-dry at -15°C for 4 hours, crush, and pass through an 80-mesh sieve to obtain a polygonatum additive.

[0074] S4.2: Mix 20 parts by mass of coffee, 2 parts by mass of polygonatum additive, and 2 parts by mass of non-dairy creamer, and stir evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage.

[0075] Comparative Example 1:

[0076] Comparative Example 1 is a commercially available coffee beverage.

[0077] Comparative Example 2:

[0078] Compared with Example 1, the difference of Comparative Example 2 is that no gardenia pectin polysaccharide is added in step S3.2. Specifically, "S3.2: add 10 parts by volume of polygonatum saponin extract to 15 parts by volume of 4wt% lecithin, and ultrasonicate until uniform to obtain a suspension", and the other steps remain unchanged. The prepared refreshing and anti-fatigue polygonatum coffee composite beverage is recorded as 2.

[0079] Comparative Example 3:

[0080] Compared with Example 1, the difference of Comparative Example 3 is that lecithin is not added in step S3.2, specifically "S3.2: adding 0.5 mol / LNaOH solution to 10 parts by volume of a 4 wt% gardenia pectin polysaccharide solution to reduce esters for 30 minutes, adding 10 parts by volume of polygonatum saponin extract, and ultrasonicating until uniform to obtain a suspension", and the other steps remain unchanged. The prepared refreshing and anti-fatigue polygonatum coffee composite beverage is recorded as 3.

[0081] Comparative Example 4:

[0082] Compared with Example 1, the difference of Comparative Example 4 is that step S4.1 is not performed, and in step S4.2, polygonatum extract gel microspheres are used instead of polygonatum additives, specifically "S4.2: 20 parts by mass of coffee, 2 parts by mass of polygonatum extract gel microspheres, and 2 parts by mass of non-dairy creamer are mixed and stirred evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage", and the other steps remain unchanged, and the prepared refreshing and anti-fatigue polygonatum coffee compound beverage is recorded as 4.

[0083] Refreshing and anti-fatigue Polygonatum coffee composite beverages were prepared by the methods of Examples 1-3 and Comparative Examples 1-4, and each method was repeated 5 times under the same environment, for a total of 7 groups of 35 beverages. The DPPH free radical scavenging rate of each Polygonatum coffee composite beverage was tested, and the average value of the 5 data in the same group was calculated. The results are shown in Table 1.

[0084] Table 1

[0085]

[0086] Refreshing and anti-fatigue Polygonatum coffee composite beverages were prepared by the methods of Examples 1-3 and Comparative Examples 1-4, and each method was repeated 5 times under the same environment, for a total of 7 groups of 35 beverages. After being sealed and stored for 3 days, the DPPH radical scavenging rate of each Polygonatum coffee composite beverage was tested, and the average value of the 5 data in the same group was calculated. The results are shown in Table 2.

[0087] Table 2:

[0088]

[0089] As can be seen from Table 1, the DPPH radical scavenging rates of Examples 1-3 are above 83.9%, while the DPPH radical scavenging rate of the commercially available coffee beverage in Comparative Example 1 is only 72.5%. It can be seen that the Polygonatum sibiricum coffee composite beverage of the present invention has better free radical scavenging ability than the commercially available product, that is, the antioxidant capacity of the present invention is better. The free radical scavenging rates of Comparative Examples 2-4 are all lower than those of the examples. It can be seen that the raw materials of the present invention have a composite synergistic effect, and the effect is better when added together than when used alone.

[0090] As can be seen from Table 2, the DPPH radical scavenging rates of the polygonatum coffee composite beverages of Examples 1-3 decreased after 3 days of storage, reaching only 68.7-69.5%, while the scavenging rates of the commercially available products of Comparative Example 1 had decreased to 53.1%, and those of Comparative Examples 2-4 also decreased to 59.8-62.4%, indicating that the antioxidant capacity of the embodiments of the present invention decreased more slowly during storage and had a longer shelf life.

[0091] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A preparation process for a refreshing and anti-fatigue polygonatum coffee compound beverage, characterized in that: The steps include: S1: Preparation of Polygonatum sibiricum Polysaccharide Weighing dry polygonatum slices, adding distilled water for extraction to obtain polygonatum polysaccharide extract, and deproteinizing the polygonatum polysaccharide extract to obtain polygonatum polysaccharide; S2: Preparation of Gardenia jasminoides pectin polysaccharide Fresh gardenia flowers are dried, crushed and sieved to obtain gardenia pollen, which is weighed and extracted with distilled water to obtain gardenia pectin polysaccharide; S3: Preparation of Polygonatum sibiricum Extract Gel Microspheres Dried polygonatum slices are crushed and sieved to obtain polygonatum powder, the polygonatum powder is soaked in ethanol to obtain a polygonatum saponin extract, the polygonatum saponin extract is mixed and dissolved with distilled water to obtain a polygonatum saponin extract solution, lecithin is added to a gardenia pectin polysaccharide solution and dispersed, and then the polygonatum saponin extract solution is added, ultrasonicated to obtain a uniform suspension, and then a calcium chloride solution is added to form gel balls to obtain polygonatum extract gel microspheres; S4: Preparation of refreshing and anti-fatigue Huangjing coffee compound beverage Polygonatum sibiricum polysaccharide is mixed with distilled water to obtain a polygonatum sibiricum polysaccharide soaking liquid, and polygonatum sibiricum extract gel microspheres are soaked in the polygonatum sibiricum polysaccharide soaking liquid to obtain a polygonatum sibiricum additive. Coffee, the polygonatum sibiricum additive and non-dairy creamer are mixed and stirred evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage.

2. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 1, characterized in that: Step S1: preparing polygonatum polysaccharide, comprising the following steps: S1.1: Weigh 5-6 parts by weight of dried Polygonatum sibiricum slices, add 10-15 parts by weight of distilled water as the extraction solvent, beat in a pulper for 5-10 minutes, remove from the pulp, and high-speed shear at a speed of 10,000-12,000 rpm for 5-10 minutes. Then, add 1-5% of the system weight of a biological enzyme, extract at a pH of 6-7 and a temperature of 50-60°C for 20-30 minutes, then inactivate the enzyme in a boiling water bath for 10-15 minutes, cool to 0-4°C, and centrifuge at 5,000-5,500 rpm for 10-15 minutes to obtain a supernatant. Concentrate the supernatant to one-fifth of the original volume to obtain a Polygonatum sibiricum polysaccharide extract; S1.2: The polysaccharide extract of polygonatum sibiricum was added to petroleum ether-acetone solvent at a material-liquid ratio of 1g: (4-5)mL and refluxed for degreasing twice, each time for 2-2.5h. After the solvent was evaporated, anhydrous ethanol 4 times the volume of the system was added and allowed to stand at 0-4℃ for 12-13h. The precipitate was collected after centrifugation and dissolved in an equal volume of distilled water. Then, 30% of the volume of the system Sevage reagent was added for deproteinization. The supernatant was collected and the above operation was repeated until there was no obvious protein between the polysaccharide phase and the lower chloroform-n-butanol phase. The defatted and deproteinized solution was dialyzed, the polysaccharide solution was collected and freeze-dried to obtain polygonatum sibiricum polysaccharide.

3. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 2, characterized in that: Step S2 is to prepare Gardenia jasminoides pectin polysaccharide, comprising the following steps: S2.1: Dry fresh gardenia flowers in the sun, grind them into powder using a high-speed multifunctional grinder, and pass them through a 40-mesh sieve for later use. Take 10-12 parts by weight of pollen and stir them with 100-110 parts by weight of 85% ethanol using an electric stirrer overnight. Filter the mixture, soak the residue again in anhydrous ethanol overnight, filter it, and then dry it to obtain the gardenia pollen. S2.2: Accurately weigh 3-5 parts by mass of gardenia pollen, add 120-130 parts by mass of distilled water, and extract for 2-3 hours at a temperature of 60-100° C. to obtain gardenia pectin polysaccharide.

4. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 3, characterized in that: Step S3 is to prepare the polygonatum extract gel microspheres, comprising the following steps: S3.1: Grind dried polygonatum slices and pass them through an 80-mesh sieve to obtain polygonatum powder. Soak the polygonatum powder in 60-70 wt% ethanol for 12-13 hours at a solid-liquid ratio of 1 g polygonatum powder to ethanol of (10-12) mL. Centrifuge for 10-15 minutes, collect the supernatant, and evaporate the ethanol solvent to obtain a polygonatum saponin extract. Mix and dissolve the polygonatum saponin extract with distilled water at a solid-liquid ratio of 1 g to (20-25) mL to obtain a polygonatum saponin extract solution. S3.2: To 10-12 parts by volume of a 4-5 wt% solution of Gardenia jasminoides pectin polysaccharide, add 0.5 mol / L NaOH solution and reduce the ester content for 30-35 minutes. Then, add 15-18 parts by volume of 4-8 wt% lecithin and disperse it. Then, add 10-15 parts by volume of a polygonatum saponin extract and sonicate until a uniform suspension is obtained. S3.3: The pH value of the calcium chloride solution is then adjusted to 2-3 with 1 mol / L HCl, and 100 parts by volume of the calcium chloride solution is dripped into the suspension through a syringe to form gel balls. The mixture is stirred for 50-60 minutes for cross-linking reaction, filtered, washed, and dried at 50-60°C for 24-25 hours to obtain polygonatum extract gel microspheres.

5. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 4, characterized in that: Step S4 is to prepare a refreshing and anti-fatigue polygonatum coffee composite beverage, comprising the following steps: S4.1: Mix polygonatum polysaccharide with distilled water at a material-liquid ratio of 1 g:(5-6) mL to obtain a polygonatum polysaccharide soak solution. Soak polygonatum extract gel microspheres in the polygonatum polysaccharide soak solution for 1-2 hours, then freeze-dry at -15°C for 3-4 hours, crush, and pass through an 80-mesh sieve to obtain a polygonatum additive. S4.2: Mix 20-25 parts by weight of coffee, 2-5 parts by weight of polygonatum additive, and 2-8 parts by weight of non-dairy creamer, and stir evenly to obtain a refreshing and anti-fatigue polygonatum coffee compound beverage.

6. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 2, characterized in that: The biological enzyme is one or more combinations of papain, cellulase and hemicellulase.

7. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 2, characterized in that: The volume ratio of petroleum ether and acetone in the petroleum ether-acetone solvent is 1:

1.

8. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 4, characterized in that: The concentration of the calcium chloride solution is 4-5 wt%.

9. The preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to claim 4, characterized in that: The syringe needle has a diameter of 0.8 mm and a needle height of 2 cm.

10. A refreshing and anti-fatigue polygonatum coffee compound beverage, characterized in that: The beverage is prepared by the preparation process of the refreshing and anti-fatigue polygonatum coffee compound beverage according to any one of claims 1 to 9.

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