Dandruff-removing, itching-relieving and hair-loss-preventing composite extract as well as preparation method and application thereof

Abstract

The invention relates to the technical field of daily chemical products, and particularly discloses a preparation method of a dandruff-removing, itching-relieving and hair-loss-preventing compound extract, which is characterized in that active ingredients in cacumen biotae, eclipta alba, radix tinosporae, radix scrophulariae, baked ginger and camellia seeds are extracted to prepare the dandruff-removing, itching-relieving and hair-loss-preventing compound extract. The invention also discloses the anti-dandruff, anti-itching and anti-alopecia composite extract and application thereof. The preparation method disclosed by the invention also has the advantages of high extract purity, strong activity and simple and easy-to-control whole process. In addition, the compound extract disclosed by the invention has the effects of removing dandruff, relieving itching, preventing alopecia and fixing hair, and is mild in action and high in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, in particular to an anti-dandruff, anti-itching and anti-hair loss composite extract, a preparation method and an application thereof. Background Art

[0002] The increasingly fast pace of modern life means that most people experience irregular sleep patterns, high levels of stress, and frequent insomnia. These issues can lead to increased oiliness on the scalp, dandruff, and, in severe cases, hair loss. Furthermore, people are increasingly exposed to various chemicals in their daily lives, further compromising the health of their hair. Without targeted treatment, these issues can become cyclical, recurring, or persistent. In severe cases, they can damage hair follicles and cause permanent hair loss.

[0003] Traditional shampoos mostly use chemically synthesized anti-dandruff and anti-hair loss agents, such as zinc pyrithione, piroctone olamine, ketoconazole, and selenium sulfide, and anti-hair loss agents like minoxidil, unoxidil, and diaminopyrimidine oxide. While these chemical ingredients can effectively remove dandruff and prevent hair loss, they are also highly irritating to the scalp, and long-term use is detrimental to scalp health.

[0004] To address these issues, a number of botanical anti-dandruff and hair loss prevention products are now available on the market. However, most of these products offer a single function: either a botanical anti-dandruff product or a botanical hair loss prevention product. Furthermore, these products are not as effective in either area as chemical shampoos. To balance these two benefits, there are also shampoos on the market that use a blend of botanical and chemical ingredients. However, these products are generally ineffective and can be irritating.

[0005] In response to the aforementioned related technologies, the inventors believe that existing chemical shampoos guarantee efficacy but suffer from high irritation, while botanical shampoos guarantee mildness but lack efficacy. Furthermore, shampoos with mixed botanical and chemical ingredients suffer from a poor balance between efficacy and irritation. Therefore, there is a need to develop a shampoo product that combines effective dandruff and hair loss prevention with gentle scalp treatments. This would address the technical bottleneck of existing shampoos, which struggle to achieve a balance between efficacy and irritation, and meet consumer demand for a safe and effective shampoo. Summary of the Invention

[0006] In order to solve the defects of the prior art, the present application provides an anti-dandruff, anti-itching and anti-hair loss composite extract, a preparation method and an application thereof.

[0007] In the first aspect, the present application provides a method for preparing a composite extract for anti-dandruff, anti-itching and anti-hair loss, using the following technical solution: A method for preparing a composite extract for removing dandruff, relieving itching and preventing hair loss comprises the following steps: S1: Mix and grind the plant raw materials, add water and boil them for 2-3 times, combine the decoctions, filter and remove the residue, and concentrate the decoctions to obtain a concentrate; The plant raw material is a compound of Platycladus orientalis leaves, Eclipta prostrata, Herba Lycopodii, Scrophulariaceae, Zingiber officinale and Camellia seeds; the mass ratio of Platycladus orientalis leaves, Eclipta prostrata, Herba Lycopodii, Scrophulariaceae, Zingiber officinale and Camellia seeds in the plant raw material is (4-6): (2.5-4.5): (1-2.5): (2-3.5): (0.5-1): (1-2); S2: adding ethanol to the concentrated solution, stirring evenly, sealing the solution, and allowing it to stand to allow impurities to settle; S3: Centrifuge the liquid after standing, take the supernatant and filter it to remove residual impurities; S4: removing the ethanol in the supernatant by distillation to obtain the anti-dandruff, anti-itching and anti-hair loss composite extract, which is then sterilized and sealed for storage.

[0008] In the above technical scheme, the present application uses water decoction to efficiently extract the active ingredients of six raw materials, purifies them by alcohol precipitation to remove impurities, ensures clarification by centrifugal filtration, distills off ethanol and sterilizes, maximizes the retention of active ingredients, and the obtained extract has high purity and strong activity. The process is simple and easy to control, and is suitable for industrial production.

[0009] Preferably, the mixed pulverization in step S1 is pulverization until the mixture passes through a 40-200 mesh sieve; the amount of water added for the decoction is 4-10 times the mass of the plant raw material, and the amount of water added decreases gradually according to the number of water additions; the density of the concentrated solution at no more than 60° C. is 1.1-1.2 g / mL.

[0010] In the above technical solution, the present application grinds the raw materials until they pass through a 40-200 mesh sieve to make the material particle size uniform and the specific surface area increased, thereby significantly improving the dissolution rate and extraction rate of effective ingredients such as Platycladus orientalis leaf flavonoids and Eclipta prostrata saponins, and avoiding component residues caused by excessively large particles; at the same time, adding enough water for the first time can ensure that the medicinal materials are fully infiltrated, break through the water absorption barrier, and promote the rapid dissolution of effective ingredients. The subsequent decreasing addition of water utilizes the concentration gradient to continuously extract residual components and improve the total extraction rate; by limiting the density of the concentrated liquid, it can prevent insufficient concentration from affecting subsequent process steps, and prevent excessive concentration from losing active substances.

[0011] Preferably, the ethanol in step S2 is 95% by volume, and the stirring is performed until the ethanol volume concentration of the concentrated solution reaches 60%-70%.

[0012] In the above technical solution, the present application uses the polarity regulation effect of medium-to-high concentrations of ethanol to precisely precipitate macromolecular impurities such as proteins and tannins, while maximally retaining medium-to-low polarity active ingredients such as Platycladus orientalis leaf flavonoids, Trichosanthes alkaloids, and Eclipta prostrata saponins. The 60%-70% ethanol concentration range avoids the problem of incomplete impurity removal with low-concentration ethanol and prevents excessive dissolution and loss of active ingredients due to high ethanol concentrations, ensuring efficient enrichment of key ingredients that inhibit bacteria and inflammation and nourish hair follicles, significantly improving the purity and biological activity of the composite extract.

[0013] Preferably, the centrifugation in step S3 is performed at a speed of 4000-5000 rpm and 4° C. for 15-20 minutes, and the filtration is performed sequentially through a pre-filtration membrane and a polyethersulfone microporous filter membrane.

[0014] In the above technical solution, this application uses centrifugation at 4000-5000 rpm and 4°C for 15-20 minutes to efficiently separate fine impurities after alcohol precipitation and protect the active ingredients from degradation; it is filtered through a pre-filter membrane and a polyethersulfone filter membrane in sequence to grade and remove large and small particle impurities, significantly improving the clarity and purity of the extract, preventing impurities from irritating the scalp or affecting the stability of the dosage form, and ensuring reliable and safe efficacy.

[0015] Preferably, the distillation in step S4 is reduced pressure distillation.

[0016] In the above technical scheme, the present invention reduces the boiling point of ethanol by vacuum distillation, quickly removes ethanol at low temperature, avoids high temperature destruction of heat-sensitive components such as Platycladus orientalis leaf flavonoids, and efficiently retains activity; at the same time, shortens the distillation time, and the obtained extract is clear and stable, taking into account both component protection and production efficiency.

[0017] In a second aspect, the present application provides a composite extract for removing dandruff, relieving itching and preventing hair loss, which adopts the following technical solution: The anti-dandruff, anti-itching and anti-hair loss composite extract is prepared by the above preparation method.

[0018] In a third aspect, the present invention applies to protect the use of the above-mentioned anti-dandruff, anti-itching and anti-hair loss composite extract in the preparation of hair care products.

[0019] Preferably, the hair care product is shampoo, conditioner, or hair mask.

[0020] In the above technical solution, the present application applies the composite extract to shampoo, conditioner, hair mask and other hair care products. Its antibacterial and anti-inflammatory ingredients can quickly remove dandruff and relieve itching, and its nourishing ingredients can improve the health of hair follicles and enhance the anchorage of hair roots. It is compatible with different dosage form matrices, has few impurities, high activity, is gentle and safe, and is suitable for a variety of scalp types.

[0021] In a fourth aspect, the present application provides a shampoo, which adopts the following technical solution: The invention comprises the above-mentioned anti-dandruff, anti-itching and anti-shedding composite extract and auxiliary materials.

[0022] In the above technical solution, the present application can flexibly adjust the formula of auxiliary materials according to actual conditions to adapt to diverse needs.

[0023] Preferably, the auxiliary materials include surfactants, conditioners, moisturizers, pH regulators, preservatives, thickeners, and solvents.

[0024] In the above technical solution, the excipients of the present application serve as compatible carriers of the extracts, which can stably carry and promote the efficient release of active ingredients to the scalp and hair shafts; at the same time, they support flexible matching and personalized adaptation for different groups of people, while ensuring the stability of the system and maximizing the anti-dandruff, anti-itching, anti-hair loss and hair strengthening effects.

[0025] In summary, this application has the following beneficial effects: 1. Through the scientific combination of raw materials such as Platycladus orientalis leaves, Eclipta prostrata, Trichosanthes kirilowii, Scrophularia ningpoensis, Zingiber officinale and Camellia japonica seeds, it can inhibit dandruff-causing fungi and relieve scalp itching while improving the nutritional supply of hair follicles and strengthening the anchorage of hair roots. The active ingredients are all plant extracts with mild medicinal properties and little irritation to the scalp.

[0026] 2. Optimized preparation processes, from raw material crushing to concentration and alcohol precipitation, ensure ingredient extraction efficiency and system stability throughout the entire process. The extract is compatible with a variety of cleaning and care product matrices, and the production process is simple and controllable, making it suitable for industrial mass production.

[0027] 3. Build an adjustable system with surfactants, conditioners and other auxiliary materials, flexibly adjust the ratio for different scalp types, and achieve personalized nourishment and repair on the basis of cleansing to meet diverse market needs. DETAILED DESCRIPTION

[0028] The present application is further described in detail below with reference to the embodiments.

[0029] The main components of Platycladus orientalis leaves are flavonoids and volatile terpenes. Flavonoids can inhibit 5α-reductase, reduce hair follicle damage, promote dermal papilla cell proliferation, and improve androgenic alopecia. Volatile oils and tannins synergistically inhibit Malassezia fungus, regulate scalp oil secretion, and relieve dandruff and itching caused by seborrheic dermatitis.

[0030] The main ingredients of Houttuynia cordata are saponins, polysaccharides and flavonoids. Saponins and polysaccharides can nourish the liver and kidneys, regulate hair loss and premature graying caused by insufficient essence and blood; flavonoids are antioxidants, delaying the aging of hair follicles, and polysaccharides enhance scalp hydration and repair dry and damaged hair barriers.

[0031] The main ingredients of Golden Fruit Olive include alkaloids such as palmatine and berberine. These ingredients have a strong inhibitory effect on Malassezia and Epidermophyton by destroying fungal cell membranes and inhibiting DNA synthesis. They can relieve inflammatory reactions such as scalp redness, swelling, and pustules, reduce scalp irritation caused by fungal metabolites, and inhibit abnormal keratinocyte shedding from the source.

[0032] The main components of Scrophularia are iridoid glycosides and phytosterols. Iridoid glycosides inhibit the androgen receptors of sebaceous gland cells, reduce oil synthesis, and improve the oily scalp environment; phytosterols have anti-inflammatory and repair effects, reduce inflammation around hair follicles, and promote keratinocyte regeneration.

[0033] The main components of roasted ginger are gingerol derivatives and a small amount of volatile oil. Gingerol dilates scalp capillaries, improves microcirculation, and accelerates nutrient delivery.

[0034] The main ingredients of camellia seeds are camellia saponins, tea polyphenols and fatty acids. Camellia saponins gently cleanse scalp oil, leaving delicate foam without irritation, making it suitable for sensitive scalps; tea polyphenols and fatty acids synergistically fight bacteria, inhibiting the growth of Malassezia and bacteria, and maintaining the balance of the microecology; unsaturated fatty acids such as oleic acid penetrate the hair, repair hair scales, form a protective film, improve dryness, frizziness and split ends, and give the hair a soft and shiny texture.

[0035] The present invention utilizes the synergistic effects of the above raw materials, so that the composite extract prepared from these six raw materials has excellent anti-dandruff, anti-itching and anti-hair loss functions. In addition, the composite extract has a mild effect and is non-irritating to the scalp, and is suitable for most people.

[0036] The present invention provides a preparation method of a composite extract for removing dandruff, relieving itching and preventing hair loss, which is specifically as follows: S1: Decoction and concentration of raw materials Combine Platycladus orientalis leaves, Eclipta prostrata, Trichosanthes kirilowii, Scrophularia ningpoensis, Zingiber officinale, and Camellia japonica seeds according to the formulated ratio and grind until it passes through a 40-200 mesh sieve. Place in an extraction tank, add 10 times the amount of deionized water, stir evenly, and heat to boiling. Reduce heat to a low boil and simmer twice, each for 1.5 hours. During the second decoction, add 6 times the amount of deionized water. Combine the two decoctions and filter under pressure through a 300-mesh filter cloth while hot to remove residue and impurities. Transfer the filtrate to a vacuum concentrator and concentrate under vacuum conditions of ≥-0.08 MPa and ≤60°C to a density of 1.1-1.2 g / mL, forming a translucent, viscous aqueous extract concentrate. Cool to room temperature and set aside.

[0037] Among the plant raw materials, the mass ratio of Platycladus orientalis leaves, Eclipta prostrata, Olea europaea, Scrophularia ningpoensis, Zingiber officinale and Camellia seeds is (4-6): (2.5-4.5): (1-2.5): (2-3.5): (0.5-1): (1-2); According to the actual quality of the raw materials, the number of decoctions can be increased to 3 times, and 4 times the amount of deionized water can be added for the third decoction.

[0038] S2: Alcohol precipitation and impurity removal and standing While stirring at 200-300 rpm, slowly add 95% ethanol to the concentrate, monitoring the ethanol concentration as you add until the final ethanol concentration reaches 60%-70% by volume. Continue stirring for 30 minutes to ensure uniform mixing. Seal the container and transfer it to a 4°C refrigerator for 12 hours to allow macromolecular impurities such as proteins, polysaccharides, and tannins to fully precipitate, forming a layered system consisting of a supernatant and a flocculent precipitate at the bottom.

[0039] S3: Centrifugal separation and precision filtration The alcohol-precipitated mixture is transferred to a centrifuge and centrifuged at 4000-5000 rpm and 4°C for 15-20 minutes to separate the upper clear liquid and the lower precipitate. The supernatant is transferred via a flow tube to a filtration device, where it passes through a 10μm pre-filter and a 0.45μm polyethersulfone microporous filter to remove any remaining fine particles and colloids, resulting in a clear, transparent alcohol-extracted mixture.

[0040] S4: Ethanol Recovery and Liquid Refining The filtrate was transferred to a rotary evaporator and distilled under reduced pressure at a vacuum degree of ≤-0.09 MPa and a water bath temperature of ≤50°C. The ethanol was recovered until the distillate had no alcohol smell. The remaining liquid was the composite extract, which was sealed and stored after sterilization.

[0041] The composite extracts of Examples 1-3 were prepared according to the formulations in the following table.

[0042] Table 1 Recipe Components Platycladus orientalis leaves Eclipta prostrata Golden Olive Scrophularia roasted ginger Camellia seeds Example 1 4 4.5 1 3.5 0.5 2 Example 2 5 2 1.5 2.5 0.5 1 Example 3 6 3 2.5 2 1 1.5 In the preparation of the composite extract of Example 1, the plant raw materials were crushed to pass through a 40-mesh sieve; Example 2: When preparing the composite extract, the plant material was crushed to pass through a 100-mesh sieve; Example 3 During the preparation of the composite extract, the plant raw materials were crushed to pass through a 200-mesh sieve.

[0043] In order to test the efficacy of the composite extract in practical applications, the composite extracts of Examples 1-3 were prepared into corresponding shampoos, corresponding to Application Examples 1-3, respectively.

[0044] The specific formulas of the shampoos of Application Examples 1-3 are shown in Table 2 below.

[0045] Table 2 Shampoo formula The preparation method of the shampoo is as follows: (1) Add most of the deionized water to the reactor (reserve some for replenishment), heat to 70–80°C, and stir evenly.

[0046] (2) Sodium laureth sulfate (AES) and cocamidopropyl betaine (CAB) were added in sequence and stirred continuously until they were completely dissolved to form a uniform system.

[0047] (3) Lower the system temperature to about 50°C, add the conditioning agent polyquaternium-10, the moisturizing agents glycerin and propylene glycol, and stir until fully mixed.

[0048] (4) Add phenoxyethanol as a preservative and carbomer as a thickener, and stir until the carbomer is evenly dispersed.

[0049] (5) Use citric acid or sodium hydroxide to adjust the pH of the system to 5.5-6.5.

[0050] (6) Add the remaining deionized water to make the total weight part 100, stir evenly, cool to room temperature, test the viscosity, pH and other indicators, and obtain the product if it meets the requirements.

[0051] The viscosity index is 8000-12000 mPa·s; the pH index is 5.5-6.5.

[0052] Comparative Example 1 The formula of the shampoo in Comparative Example 1 is basically the same as the formula of the shampoo in Application Examples 1-3, except that 2% by mass of pyrithione zinc is used instead of the composite extract, and the missing part is supplemented by deionized water. The preparation method of the shampoo in Comparative Example 1 is the same as the preparation method of Application Examples 1-3.

[0053] Comparative Example 2 The formula of the shampoo in Comparative Example 2 is basically the same as the formula of the shampoo in Application Examples 1-3, except that 0.2% by mass of benzylchlorophenol is used instead of the composite extract, and the missing part is supplemented by deionized water. The preparation method of the shampoo in Comparative Example 2 is the same as the preparation method of the shampoo in Application Examples 1-3.

[0054] Comparative Example 3 The formula of the shampoo in Comparative Example 3 is basically the same as that of the shampoo in Application Examples 1-3, except that 10% by mass of plant extract is used instead of the composite extract. The preparation method of the shampoo in Comparative Example 3 is the same as that of the shampoo in Application Examples 1-3.

[0055] The preparation method of the plant extract in Comparative Example 3 is consistent with the preparation method of the composite extract in Example 3 of the present invention, except that the raw materials of the plant extract in Comparative Example 3 are composed of 6 parts of ginger, 5 parts of sapodilla, 5 parts of arborvitae leaves, 3 parts of ginseng roots, 3 parts of Polygonum multiflorum, 3 parts of angelica, 2 parts of ginkgo, 1 part of Stemona, 1 part of Cnidium monnieri, 1 part of Dictamni and 1 part of chrysanthemum leaves, in parts by mass.

[0056] Comparative Example 4 The formula of the shampoo in Comparative Example 4 is basically the same as that of the shampoo in Application Examples 1-3, except that 2% by mass of a plant extract and 4% by mass of a sophorolipid are used instead of the composite extract, and the missing part is filled with deionized water. The preparation method of the shampoo in Comparative Example 4 is the same as that of the shampoo in Application Examples 1-3.

[0057] The sophorolipids in this comparative example are secondary metabolites produced by fermenting glucose and rapeseed oil with ATCC 22214 Candida species, and the fermentation method is a conventional fermentation tank fermentation method.

[0058] The raw materials of the plant extract in this comparative example are composed of 9 parts of rosemary leaves and 1 part of Platycladus orientalis leaves in parts by mass. The preparation method of the plant extract is basically the same as the preparation method of the composite extract described in Example 3 of the present invention, except that the amount of water added in step S1 is 5 times.

[0059] Comparative Example 5 The formula of the shampoo in Comparative Example 5 is basically the same as the formula of the shampoo in Application Examples 1-3, except that 9% by mass of plant extract and 1% by mass of pyrithione zinc are used instead of the composite extract. The preparation method of the shampoo in Comparative Example 5 is the same as the preparation method of the shampoo in Application Examples 1-3.

[0060] The preparation method of the plant extract in Comparative Example 5 is consistent with the preparation method of the composite extract in Example 3 of the present invention, except that the raw materials of the plant extract in Comparative Example 5 are composed of 6 parts of moutan bark, 7 parts of kudingcha, 3 parts of salvia miltiorrhiza, 5 parts of turmeric, 3 parts of angelica, 5 parts of glossy privet, 3 parts of lavender, 6 parts of eclipta, 4 parts of arborvitae leaves, 3 parts of cnidium monnieri and 5 parts of lilac flowers, in parts by mass.

[0061] Comparative Example 6 The formula of the shampoo in Comparative Example 6 is basically the same as the formula of the shampoo in Application Examples 1-3, except that 9% by mass of plant extract and 1% by mass of pyrithione zinc are used instead of the composite extract. The preparation method of the shampoo in Comparative Example 6 is the same as the preparation method of the shampoo in Application Examples 1-3.

[0062] The preparation method of the plant extract in Comparative Example 6 is consistent with the preparation method of the composite extract in Example 3 of the present invention, except that the raw materials of the plant extract in Comparative Example 6 are 6 parts of ginkgo leaves, 6 parts of angelica sinensis and 6 parts of Composed of ginseng root.

[0063] Comparative Example 7 The formula of the shampoo in Comparative Example 7 is basically the same as that of the shampoo in Application Examples 1-3, except that 2% by mass of minoxidil is used instead of the composite extract, and the missing portion is filled with deionized water. The preparation method of the shampoo in Comparative Example 7 is the same as that of the shampoo in Application Examples 1-3.

[0064] Comparative Example 8 The formula of the shampoo in Comparative Example 8 is basically the same as that of the shampoo in Application Examples 1-3, except that 10% by mass of plant extract is used instead of the composite extract. The preparation method of the shampoo in Comparative Example 8 is the same as that of the shampoo in Application Examples 1-3.

[0065] The preparation method of the plant extract in Comparative Example 8 is consistent with the preparation method of the composite extract in Example 3 of the present invention, except that the raw materials of the plant extract in Comparative Example 8 are composed of 20 parts of Platycladus orientalis leaves, 7 parts of Polygonum multiflorum, 5 parts of Cuscuta seeds, 10 parts of Kaempferia galanga, 10 parts of Chuanxiong, 2 parts of Licorice, 20 parts of Artemisia argyi, 4 parts of Sophora flavescens, 6 parts of Ligustrum lucidum, 3 parts of Zanthoxylum bungeanum and 3 parts of dried tangerine peel, in parts by mass.

[0066] Comparative Example 9 The formula of the shampoo in Comparative Example 9 is basically the same as that of the shampoo in Application Examples 1-3, except that 8.5% by mass of plant extract and 1.5% by mass of diaminopyrimidine oxide are used instead of the composite extract. The preparation method of the shampoo in Comparative Example 8 is the same as that of the shampoo in Application Examples 1-3.

[0067] The preparation method of the plant extract in Comparative Example 9 is consistent with the preparation method of the composite extract in Example 3 of the present invention, except that the raw materials of the plant extract in Comparative Example 8 are 4 parts of ginger, 6 parts of Platycladus orientalis leaves, and 4 parts of It is composed of ginseng root, 4 parts of Polygonum multiflorum, 3 parts of Sophora flavescens root and 3 parts of Camellia seed.

[0068] Among them, Comparative Examples 1-6 are anti-dandruff shampoos, and Comparative Examples 7-9 are anti-hair loss shampoos. Specifically, Comparative Examples 1-2 are chemical anti-dandruff shampoos, Comparative Examples 3-4 are plant-based anti-dandruff shampoos, and Comparative Examples 5-6 are mixed-ingredient anti-dandruff shampoos. Comparative Example 7 is a chemical anti-hair loss shampoo, Comparative Example 8 is a plant-based anti-hair loss shampoo, and Comparative Example 9 is a mixed-ingredient anti-hair loss shampoo.

[0069] Performance Testing 1. Security testing Refer to the human skin patch test in the 2022 edition of the "Safety Technical Specifications for Cosmetics" to test whether the above-mentioned example compositions cause skin irritation or allergic reactions. The specific experimental steps are as follows: (1) 90 people aged 18-60 were selected and divided into 3 groups, corresponding to application examples 1-3, with 15 males and 15 females in each group, all of whom had no severe skin diseases, allergies or immune diseases; (2) The selected area shall not exceed 50mm 2 , a qualified patch test device with a depth of approximately 1mm. 0.02mL of the shampoo from Application Examples 1-3 was placed into the patch test chamber. The control well served as a blank control (no substance was placed). The patch tester containing the finished product was applied to the healthy skin on both sides of the spine on the back of the subject using hypoallergenic tape. Gently press the patch evenly with the palm of your hand for 24 hours. Skin reactions were observed 30 minutes after removal of the finished product (after the indentation disappears), 24 hours, and 48 hours later, and the results were recorded. The results are shown in Table 3 below, and the specific standards for the results are shown in Table 4 below.

[0070] Table 3 Human skin patch test results serial number 30min 24h 48h Application Example 1 Level 0, 30 people Level 0, 30 people Level 0, 30 people Application Example 2 Level 0, 30 people Level 0, 30 people Level 0, 30 people Application Example 3 Level 0, 30 people Level 0, 30 people Level 0, 30 people Table 4 Specific standards for reaction results Reaction level Symptom description 0 No response 1+ Erythema, infiltration, papules 2+ Erythema, infiltration, papules, blisters 3+ Severe erythema, bullae, and ulcers As shown in Tables 2 and 3, all test subjects had a grade 0 reaction, i.e., no allergic reaction occurred. This shows that the shampoo prepared from the composite extract of the present invention has low irritation to the skin, mild effect, and good safety.

[0071] 2. Zein irritation test: The shampoos of Application Examples 1-3 and Comparative Examples 1-9 were each prepared into test solutions with an active ingredient mass fraction of 10%. 50 mL of the test solution was added with 2 g of zein. Stir at 25°C for 2 hours, observing the solution every 10 minutes and adding zein as needed to ensure a sufficient amount of undissolved zein. The solution was then allowed to stand and filtered, and the nitrogen mass fraction (A) in the filtrate was measured using the micro-Kjeldahl method. Another 50 mL was taken and a blank experiment was performed without the addition of zein to determine the nitrogen mass fraction (B) in the detergent solution without zein.

[0072] Zein value = AB, Zein value is expressed by the number of grams of nitrogen contained in the solution, N g / L.

[0073] Judgment basis: Zein>4 means strong irritation; 2<Zein<4 means low irritation; Zein<2 means no irritation.

[0074] The test results are shown in Table 5 below: Table 5 Zein irritation test results sample Zein value (g / L) Application Example 1 Shampoo 0.01 Application Example 2: Shampoo 0.01 Application Example 3 Shampoo 0.01 Comparative Example 1 Shampoo 3.28 Comparative Example 2 Shampoo 5.62 Comparative Example 3 Shampoo 0.01 Comparative Example 4 Shampoo 0.01 Comparative Example 5 Shampoo 1.87 Comparative Example 6 Shampoo 1.45 Comparative Example 7 Shampoo 2.51 Comparative Example 8 Shampoo 0.01 Comparative Example 9 Shampoo 1.17 As can be seen from Table 5, the shampoos of Examples 1-3 of the present invention are mildly irritating and appear non-irritating, while the chemical anti-dandruff shampoos of Comparative Examples 1-2 are highly irritating, with Zein values greater than 2. Comparative Example 1 exhibits low irritation, while Comparative Example 2 exhibits strong irritation. The plant-based anti-dandruff shampoos of Comparative Examples 3-4 are mildly irritating. While the mixed-ingredient anti-dandruff shampoos of Comparative Examples 5-6 appear non-irritating, their Zein values are closer to 2, making them more irritating than the shampoos of Examples 1-3 of the present invention. Furthermore, among the anti-hair loss shampoos, Comparative Example 7 exhibits low irritation, while Comparative Examples 8 and 9 exhibit non-irritation, reflecting the greater irritation of chemical ingredients relative to plant ingredients.

[0075] 3. Malassezia furfur antibacterial test The generation of dandruff is relevant with the excessive breeding of Malassezia furfur to a great extent.When Malassezia furfur breeds in large quantities, it can decompose oil and produce fatty acids. These fatty acids irritate the scalp, causing skin cells to fall off faster and form dandruff.At the same time, Malassezia furfur can also cause scalp inflammation, aggravating the dandruff problem.In the present embodiment, the mode of adopting inhibition zone experiment is tested the effect that the present invention suppresses Malassezia furfur.

[0076] The principle of the inhibition zone experiment is to use the antibacterial agent to dissolve in the culture medium and continuously diffuse to form different concentration gradients to show its antibacterial effect. The size of the inhibition zone is used to judge whether it has antibacterial ability.

[0077] In order to further verify the ability of the composite extract of the present invention to inhibit Malassezia furfur in actual products, the plate punch method was used in this embodiment to test the antibacterial ability of the sample. The specific experimental steps are as follows: (1) Use a sterile cotton swab to 4 Spread the Malassezia furfur at cfu / ml evenly on the surface of the nutrient agar plate medium and let it dry at room temperature for 10 minutes.

[0078] (2) Use a sterile circular punch with a diameter of 6 mm to punch holes. Take 2 ml of each of the shampoos from Application Examples 1-3 and Comparative Examples 1-9, diluted 100-fold with normal saline, and add them to the holes until they are full without overflow. Perform three parallel experiments.

[0079] (3) The culture dish was placed in a constant temperature incubator at (37 ± 1) °C for 24 h. The dish was removed for observation and the diameter of the inhibition zone (mm) was measured with a vernier caliper. The outer edge of the inhibition zone where no colonies grew was used as the boundary. The results were determined according to the National Committee for Clinical Trials Standards (NCCLS) standard: an inhibition zone diameter of less than 8 mm was considered insensitive, 8-13 mm was considered lowly sensitive, 13-19 mm was considered moderately sensitive, and greater than 19 mm was considered highly sensitive. The experimental results are shown in Table 6 below.

[0080] Table 6 Malassezia furfur inhibition test results sample Inhibition zone diameter / mm Application Example 1 Shampoo 21.24 Application Example 2: Shampoo 21.88 Application Example 3 Shampoo 22.45 Comparative Example 1 Shampoo 27.24 Comparative Example 2 Shampoo 30.05 Comparative Example 3 Shampoo 18.82 Comparative Example 4 Shampoo 17.35 Comparative Example 5 Shampoo 22.41 Comparative Example 6 Shampoo 19.84 Comparative Example 7 Shampoo 7.74 Comparative Example 8 Shampoo 14.21 Comparative Example 9 Shampoo 15.53 From the experimental results in Table 6, it can be seen that the diameters of the inhibition zones corresponding to the shampoos of Application Examples 1-3 of the present invention are 21.24-22.45 mm, all greater than 19 mm, which are highly sensitive and have strong anti-dandruff ability; the diameters of the inhibition zones of Comparative Examples 1-2 (chemical anti-dandruff shampoo) are 27.24 mm and 30.05 mm, both greater than 19 mm, which are highly sensitive and have stronger anti-dandruff ability; the diameters of the inhibition zones of Comparative Examples 3-4 (plant-based anti-dandruff shampoo) are 18.82 mm and 17.35 mm, which are both greater than 19 mm, which are highly sensitive and have stronger anti-dandruff ability. In the range of 13-19mm, it is moderately sensitive and has an average anti-dandruff ability; the diameters of the inhibition zones of comparative examples 5-6 (mixed anti-dandruff shampoo) are 22.41mm and 19.84mm, both greater than 19mm, and are highly sensitive; the diameters of the inhibition zones of comparative examples 7-9 (anti-hair loss shampoo) are 7.74mm, 14.21mm, and 15.53mm, the former being less than 8mm, indicating insensitivity and weak anti-dandruff ability, while the latter two are in the range of 13-19mm, indicating moderate sensitivity and average anti-dandruff ability. Thus, although the anti-dandruff ability of the shampoo in application examples 1-3 of the present invention is slightly inferior to that of the chemical anti-dandruff shampoo in comparative examples 1-2, it is significantly better than the plant-based anti-dandruff shampoo in comparative examples 3-4 and the anti-hair loss shampoo in comparative examples 7-9, and performs well in anti-dandruff ability, showing a good anti-dandruff effect.

[0081] 4. Anti-dandruff and anti-itching efficacy test method: 240 volunteers with dandruff and oily hair, aged between 15 and 60 years old, were selected and randomly divided into 12 groups, 20 people in each group. They were given the above test samples and shampooed once every morning. After shampooing for four weeks, the oiliness of the hair was observed on the morning of the second day.

[0082] The effect evaluation criteria are divided into four levels: markedly effective, effective, improved, and ineffective. The specific criteria are as follows: Markedly effective: Visible dandruff is greatly reduced to almost invisible, and the patient feels that the itchy scalp symptoms have completely disappeared; Effective: Dandruff is significantly reduced and the patient feels that the itchy scalp symptoms have basically disappeared; Improvement: Visible dandruff is slightly reduced, and the patient feels that the itchy scalp symptoms are alleviated; Ineffective: The amount of dandruff visible to the naked eye is almost unchanged, and the patient feels no improvement in the itchy scalp symptoms.

[0083] The evaluation results are shown in Table 7 below: Table 7 Evaluation results of anti-dandruff and antipruritic efficacy The experimental results in Table 7 show that the shampoos in Application Examples 1-3 of the present invention have good anti-dandruff and anti-itching effects, with a total effective rate of over 90%. This data is slightly weaker than the chemical anti-dandruff shampoo in Comparative Examples 1-2, and is basically the same as the mixed-ingredient anti-dandruff shampoo in Comparative Examples 5-6, and higher than the plant-based shampoo in Comparative Examples 3-4 and the anti-hair loss shampoo in Comparative Examples 7-9. This shows that the composite extract shampoo of the present invention has made significant progress in anti-dandruff and anti-itching efficacy compared to other plant-based anti-dandruff shampoos, and its anti-dandruff and anti-itching ability is close to that of traditional chemical-based shampoos.

[0084] 4. Anti-hair loss efficacy test The present invention randomly selected 240 individuals with hair loss aged between 18 and 60, including 201 males and 99 females. The 300 test subjects were then randomly divided into 12 groups of 20 each, each receiving the shampoos of Example 1-3 and Comparative Example 1-9, respectively. The treatment cycle was three months, with a frequency of twice weekly use. The test results were categorized into three levels: markedly effective, effective, and ineffective, with the following specific criteria: Significantly effective: refers to the combing test where the number of hairs lost is reduced by more than 90% compared to before use; Effective: refers to the combing test where the number of hairs lost is reduced by 30% to 60% compared to before use; Ineffective: refers to the combing test where the number of hairs lost is reduced by less than 30% compared to before use.

[0085] The test results of the hair loss group are shown in Table 8: Table 8 Anti-hair loss efficacy test results The test results in Table 7 show that the total effective rate of preventing hair loss using the shampoos in Application Examples 1-3 of the present invention is all above 85%, which is slightly lower than the anti-hair loss shampoos in Comparative Examples 7-9, but significantly higher than the anti-dandruff shampoos in Comparative Examples 1-6. This shows that the shampoos prepared from the composite extracts of the present invention have good anti-hair loss efficacy.

[0086] In summary, the composite extract extracted from plant raw materials in the present invention has an anti-dandruff ability close to that of traditional chemical anti-dandruff agents and an anti-hair loss ability close to that of traditional chemical anti-hair loss agents. While having the effects of anti-dandruff, anti-itching and anti-hair loss, the shampoo prepared from the composite extract of the present invention also retains the advantages of mildness and low irritation of plant ingredients, overcoming the limitations of traditional chemical formula shampoos, plant formula shampoos and mixed formula shampoos, and providing a safe and efficient innovative solution for scalp care.

[0087] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A method for preparing a composite extract for anti-dandruff, anti-itching and anti-shedding, characterized in that: Including the following step: S1: Mix and grind the plant materials, add water and boil them for 2-3 times, combine the decoctions, filter and remove the residue, and concentrate the decoctions to obtain a concentrate; The plant raw material is a compound of Platycladus orientalis leaves, Eclipta prostrata, Trichosanthes kirilowii, Scrophularia ningpoensis, Zingiber officinale and Camellia japonica seeds; the mass ratio of Platycladus orientalis leaves, Eclipta prostrata, Trichosanthes kirilowii, Zingiber officinale and Camellia japonica seeds is (4-6): (2.5-4.5): (1-2.5): (2-3.5): (0.5-1): (1-2); S2: adding ethanol to the concentrated solution, stirring evenly, sealing the solution, and allowing it to stand to allow impurities to settle; S3: Centrifuge the liquid after standing, take the supernatant and filter it to remove residual impurities; S4: removing the ethanol in the supernatant by distillation to obtain the anti-dandruff, anti-itching and anti-hair loss composite extract, which is then sterilized and sealed for storage.

2. A preparation method according to claim 1, characterized in that: The mixed pulverization in step S1 is pulverized until it passes through a 40-200 mesh sieve; the amount of water added for the decoction is 4-10 times the mass of the plant material, and the amount of water added decreases gradually according to the number of times water is added; the density of the concentrated solution at no more than 60° C. is 1.1-1.2 g / mL.

3. A preparation method according to claim 1, characterized in that: The ethanol in step S2 is 95% by volume, and the stirring is performed until the ethanol volume concentration of the concentrated solution reaches 60%-70%.

4. A preparation method according to claim 1, characterized in that: The centrifugation condition in the step S3 is 4000-5000 rpm at 4° C. for 15-20 minutes, and the filtration is performed by filtering through a pre-filtration membrane and a polyethersulfone microporous filter membrane in sequence.

5. A preparation method according to claim 1, characterized in that: The distillation in step S4 is a reduced pressure distillation.

6. A composite extract for anti-dandruff, anti-itching and anti-shedding, characterized in that: The anti-dandruff, anti-itching and anti-shedding The combined extract is prepared by the preparation method according to any one of claims 1 to 5.

7. Use of the anti-dandruff, anti-itching and anti-hair loss composite extract according to claim 6 in the preparation of hair care products.

8. The application according to claim 7, characterized in that: The hair care products include shampoo, conditioner and hair mask.

9. A shampoo, characterized in that: The invention comprises the anti-dandruff, anti-itching and anti-shedding composite extract according to claim 1 and auxiliary materials.

10. The shampoo according to claim 9, characterized in that: The auxiliary materials include surfactants, conditioners, moisturizers, pH regulators, preservatives, thickeners, and solvents.

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