Plant extract with triple effects of inhibiting bacteria, resisting oxidation and relieving itching, composition as well as preparation method and application of plant extract

Abstract

The invention provides a plant extract with triple effects of inhibiting bacteria, resisting oxidation and relieving itching, a composition and a preparation method and application of the plant extract and the composition, and relates to the technical field of daily chemical products. A comprises a myrobalan fruit extract; b is a plum fruit extract, or at least one of a rose flower extract, a Chinese rose flower extract or a tribulus terrestris fruit extract and the plum fruit extract; when b is the plum fruit extract, the weight ratio of a to b is (2-4): 1; when b is at least one of the rose extract, the China rose extract or the tribulus terrestris fruit extract and the plum fruit extract, the weight ratio of a to b is 1: 1. The composition can play good antibacterial, antioxidant and antipruritic roles and realize a relatively strong synergistic effect, so that a better effect can be achieved when the composition is applied to a skin care product. The technical problem that in the prior art, a plant extract capable of simultaneously achieving the effects of inhibiting bacteria, resisting oxidation and relieving itching is lacked is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and particularly to a plant extract, a composition having antibacterial, antioxidant and antipruritic triple functions, and a preparation method and application thereof. Background Art

[0002] Pityriasis capitis, commonly known as dandruff, can cause skin itching, flaking, and is accompanied by a mild inflammatory reaction. The occurrence of dandruff is closely related to the disorder of the scalp barrier function. According to the main functions of the scalp barrier, it can be divided into a penetration barrier, an antimicrobial barrier, an antioxidant barrier, an immune response barrier, and a light protection barrier. When these functional barriers are abnormal, they will interfere with the normal physiological state of the scalp. If not repaired in time, it may trigger scalp problems or diseases. Especially the antimicrobial barrier and the antioxidant barrier, which play a key role in resisting the invasion of pathogens and reducing scalp cell damage (slowing down scalp aging). Therefore, these two barriers are particularly crucial in the prevention and treatment of dandruff. In addition, Malassezia secretes lipase to decompose triglycerides and saturated fatty acids in sebum into unsaturated fatty acids, which destroys the stratum corneum barrier and causes inflammation and itching. In addition, acne, athlete's foot, tinea, dermatitis, psoriasis, etc. are all related to the damage of the antimicrobial barrier, the damage of the antioxidant barrier, and itching.

[0003] Common chemical anti-dandruff agents on the market include salicylic acid, selenium disulfide, zinc pyrithione (ZPT), octopirox ethanolamine salt (OCT), clotrimazole, etc. Salicylic acid is a stratum corneum exfoliant, without obvious antifungal efficacy, with poor anti-dandruff effect, large irritation, and easy to exfoliate the skin and cause damage; selenium disulfide is a cell growth inhibitor, with strong irritation, and it will feel dry after use, and long-term retention will cause hair loss and odor; zinc pyrithione (ZPT, has been banned in the EU), octopirox ethanolamine salt (OCT), clotrimazole, ketoconazole, etc. all belong to potent fungicides, and all have the disadvantages of large irritation and being unfriendly to the environment. Moreover, long-term use of chemical reagents will cause drug resistance and harm to the immune system.

[0004] In recent years, plant-derived products for treating dandruff, acne, etc. have attracted much attention due to their small side effects and low drug resistance. However, the efficacy of the currently used plant extracts is limited and it is difficult to meet the complex and changeable scalp needs.

[0005] In view of this, the present invention is specifically proposed. Summary of the Invention

[0006] One of the purposes of the present invention is to provide a plant extract having antibacterial, antioxidant and antipruritic triple functions, so as to solve the technical problem in the prior art that there is a lack of a plant extract that can simultaneously have antibacterial, antioxidant and antipruritic triple functions.

[0007] The second object of the present invention is to provide the preparation method of the above-mentioned plant extract.

[0008] The third object of the present invention is to provide a composition having triple functions of antibacterial, antioxidant and antipruritic.

[0009] The fourth object of the present invention is to provide the preparation method of the above-mentioned composition.

[0010] The fifth object of the present invention is to provide the application of the above-mentioned plant extract, the plant extract prepared by the above-mentioned preparation method, the above-mentioned composition or the composition prepared by the above-mentioned preparation method in the preparation of scalp care products or hair care products.

[0011] In order to achieve the above objects of the present invention, the following technical solutions are specifically adopted: In the first aspect, the present invention provides a plant extract having triple functions of antibacterial, antioxidant and antipruritic, and the plant extract includes a and b; a includes Terminalia chebula Retz. fruit extract; b is Prunus mume fruit extract, or at least one of Rosa rugosa Thunb. extract, Rosa chinensis Jacq. extract or Tribulus terrestris L. fruit extract and Prunus mume fruit extract; When b is Prunus mume fruit extract, the weight ratio of a to b is 2-4:1; When b is at least one of Rosa rugosa Thunb. extract, Rosa chinensis Jacq. extract or Tribulus terrestris L. fruit extract and Prunus mume fruit extract, the weight ratio of a to b is 1:1.

[0012] Further, b is Prunus mume fruit extract.

[0013] Further, b is Prunus mume fruit extract and flower extract; The weight ratio of the Prunus mume fruit extract to the flower extract is 1:1; the flower extract is Rosa rugosa Thunb. extract or Rosa chinensis Jacq. extract.

[0014] Further, b is Prunus mume fruit extract, flower extract and Tribulus terrestris L. fruit extract; The weight ratio of the Prunus mume fruit extract, the flower extract and the Tribulus terrestris L. fruit extract is 10:7-9:1-3; the flower extract includes Rosa rugosa Thunb. extract or Rosa chinensis Jacq. extract.

[0015] In the second aspect, the present invention provides the preparation method of the above-mentioned plant extract, including mixing and soaking the plant raw materials of each component with an extractant according to a volume ratio of 1:8-12, ultrasonic treatment, taking the filtrate for concentration, centrifuging to obtain the supernatant to obtain a single plant extract, and mixing according to the formula to obtain the plant extract; Preferably, the extractant includes 50%-80% ethanol, 30%-80% butylene glycol or water; Preferably, the ultrasonic treatment time is 30-90 min; Preferably, the soaking time is 0.5 - 2.5 h.

[0016] In a third aspect, the present invention provides a composition having triple functions of antibacterial, antioxidant and antipruritic, comprising the above-mentioned plant extract or the plant extract prepared by the above-mentioned preparation method, and hinokitiol, wherein the weight ratio of the plant extract to hinokitiol is 1 - 30:1.

[0017] Furthermore, the weight ratio of the plant extract to hinokitiol is 1 - 3:1.

[0018] In a fourth aspect, the present invention provides a preparation method of the above-mentioned composition, comprising mixing a hinokitiol alcohol solution with a plant extract according to the formula.

[0019] Furthermore, the concentration of hinokitiol in the hinokitiol alcohol solution is 1 - 200 mg / mL, preferably 50 mg / mL.

[0020] In a fifth aspect, the present invention provides the application of the above-mentioned plant extract, the plant extract prepared by the above-mentioned preparation method, the above-mentioned composition or the composition prepared by the above-mentioned preparation method in the preparation of scalp care products or hair care products.

[0021] A plant extract having triple functions of antibacterial, antioxidant and antipruritic provided by the present invention, when the Terminalia chebula fruit extract is compounded with the Prunus mume fruit extract, the Rosa rugosa flower extract, the Rosa chinensis flower extract or the Tribulus terrestris fruit extract within a certain weight ratio range, they can cooperate with each other to exert good antibacterial, antioxidant and antipruritic effects, achieving a strong synergistic effect, so that better effects can be achieved when it is applied to skin care products. To solve the technical problem in the prior art that there is a lack of a plant extract that can simultaneously have triple functions of antibacterial, antioxidant and antipruritic. On the other hand, a composition having triple functions of antibacterial, antioxidant and antipruritic provided, when the above-mentioned plant extract is compounded with hinokitiol in a certain weight ratio, each component acts on skin problems, especially scalp problems, through multiple targets and multiple dimensions, can significantly improve the antibacterial, antioxidant and antipruritic effects of the composition, thereby protecting the overall health of the skin. And it shows better antibacterial, antioxidant and antipruritic effects than the chemical anti-dandruff agent OCT and hinokitiol used alone under the defined ratio. Specific Embodiments

[0022] Unless otherwise defined herein, scientific and technical terms used in conjunction with the present invention shall have the meanings commonly understood by those of ordinary skill in the art. The meanings and scopes of the terms should be clear. However, in any case of potential ambiguity, the definitions provided herein shall prevail over any dictionary or extrinsic definition. In this application, unless otherwise specified, the use of "or" means "and / or". In addition, the use of the term "comprising" and other forms is non-restrictive.

[0023] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0024] On the one hand, the present invention provides a plant extract having triple functions of antibacterial, antioxidant and antipruritic. The plant extract includes a and b; a includes Terminalia chebula fruit extract; b is Prunus mume fruit extract, or at least one of Rosa rugosa flower extract, Rosa chinensis flower extract or Tribulus terrestris fruit extract and Prunus mume fruit extract; When b is Prunus mume fruit extract, the weight ratio of a to b is 2-4:1; When b is at least one of Rosa rugosa flower extract, Rosa chinensis flower extract or Tribulus terrestris fruit extract and Prunus mume fruit extract, the weight ratio of a to b is 1:1.

[0025] When Terminalia chebula fruit extract is compounded with Prunus mume fruit extract, Rosa rugosa flower extract, Rosa chinensis flower extract or Tribulus terrestris fruit extract within a certain weight ratio range and they cooperate with each other, they can exert good antibacterial, antioxidant and antipruritic effects, achieving a strong synergistic effect. Therefore, when applied to skin care products, better effects can be achieved. This solves the technical problem in the prior art that there is a lack of plant extracts that can simultaneously have triple functions of antibacterial, antioxidant and antipruritic.

[0026] In some specific embodiments, b is Prunus mume fruit extract.

[0027] In some specific embodiments, b is Prunus mume fruit extract and flower extract, and the weight ratio of Prunus mume fruit extract to flower extract is 1:1. In some specific embodiments, the flower extract is one of Rosa rugosa flower extract or Rosa chinensis flower extract.

[0028] In some specific embodiments, b is Prunus mume fruit extract, flower extract and Tribulus terrestris fruit extract, and the weight ratio of Prunus mume fruit extract, flower extract and Tribulus terrestris fruit extract is 10:7-9:1-3.

[0029] Among them, the weight ratio of Prunus mume fruit extract, flower extract and Tribulus terrestris fruit extract can be but is not limited to 10:7:1, 10:7:2, 10:7:3, 10:8:1, 10:8:2, 10:8:3, 10:9:1 or 10:9:3, and can also be any value between 10:7-9:1-3.

[0030] In some specific embodiments, the flower extract is one of rose flower extract or Chinese rose flower extract.

[0031] According to another aspect of the present invention, there is also provided a method for preparing the above-mentioned plant extract, which includes mixing and soaking the plant raw materials of each component with an extractant in a volume ratio of 1:8 to 12, performing ultrasonic treatment, taking the filtrate for concentration, centrifuging to obtain the supernatant to obtain a single plant extract, and mixing according to the formula to obtain the plant extract.

[0032] In some specific embodiments, the extractant includes 50% - 80% ethanol, 30% - 80% butanediol or water.

[0033] Among them, the concentration of the ethanol can be, but is not limited to, 50%, 55%, 60%, 65%, 70%, 75% or 80%, and can also be any value between 50% and 80%.

[0034] The concentration of the butanediol can be, but is not limited to, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75% or 80%, and can also be any value between 30% and 80%.

[0035] In some specific embodiments, the time of the ultrasonic treatment is 30 - 90 min; in some specific embodiments, the time of the soaking is 0.5 - 2.5 h.

[0036] According to another aspect of the present invention, there is also provided a composition having triple functions of bacteriostasis, antioxidant and antipruritic, which includes the above-mentioned plant extract or the plant extract prepared by the above-mentioned preparation method, and hinokitiol, and the weight ratio of the plant extract to hinokitiol is 1 - 30:1.

[0037] When the above-mentioned plant extract and hinokitiol are compounded in a certain weight ratio, each component acts on skin problems, especially scalp problems, through multiple targets and multiple dimensions, can significantly improve the bacteriostasis, antioxidant and antipruritic effects of the composition, thereby protecting the overall health of the skin. And it shows better bacteriostasis, antioxidant and antipruritic effects than the chemical anti-dandruff agent OCT and hinokitiol used alone under the defined ratio.

[0038] Among them, the weight ratio of the plant extract to hinokitiol can be, but is not limited to, 1:1, 1.3:1, 1.5:1, 1.8:1, 2:1, 2.3:1, 2.5:1, 2.8:1, 3:1, 5:1, 10:1, 15:1, 20:1, 25:1 or 30:1, and can also be any value between 1 and 30:1, preferably 1 - 3:1.

[0039] According to another aspect of the present invention, there is also provided a method for preparing the above composition, which includes mixing the hinokitiol solution and the plant extract according to the formula.

[0040] In some specific embodiments, the concentration of hinokitiol in the hinokitiol solution in the above preparation method is 1 - 200 mg / mL, preferably 50 mg / mL.

[0041] According to another aspect of the present invention, there is also provided the use of the above plant extract, the plant extract prepared by the above preparation method, the above composition or the composition prepared by the above preparation method in the preparation of scalp care products or hair care products.

[0042] Specifically, the product has at least one of the functions of antibacterial, antipruritic or antioxidant.

[0043] The present invention will be further illustrated by the following examples. Unless otherwise specified, the materials in the examples are prepared according to the existing methods or directly purchased from the market.

[0044] Example 1 Plant Extract with Antibacterial, Antipruritic and Antioxidant Triple Effects According to the components of the plant extract, plant extracts with different component combinations are provided, and the weight ratios of the specific combined components are shown in Table 1.

[0045] Table 1 Proportion of Combined Components of Plant Extract

[0046] The above plant extract is specifically prepared according to the following steps: (1) Weigh 15 g of the plant medicinal materials of each component respectively, add 10 times the amount of 70% ethanol and soak for 1 h, extract by ultrasound 2 times, each time for 20 min, combine the two filtrates and concentrate to 1 g / mL, centrifuge to obtain the plant supernatant of each component, which is a single plant extract, and store it at 4°C for later use; (2) Dilute the total concentration of the above obtained plant supernatant to 200 mg / mL, without changing the total concentration, adjust the proportion of each component according to Table 1 to obtain the plant extract.

[0047] Example 2 A Composition with Antibacterial, Antipruritic and Antioxidant Triple Effects 1. A composition composed of plant extract and hinokitiol According to the components of the composition, compositions with different component combinations are provided, and the weight ratios of the composition formulas are shown in Table 2.

[0048] Table 2 Proportion of Combined Components of Plant Extract and Hinokitiol

[0049] The compositions in Table 2 were prepared specifically according to the following steps: (1) Dissolve hinokitiol in ethanol or butanediol (0.2% Tween is used to assist dissolution), and prepare a hinokitiol alcohol solution with a concentration of 50 mg / mL as the hinokitiol stock solution; seal the nozzle of the stock solution with a sealing film, and store it in a 4°C refrigerator in the dark for later use; (2) Mix the plant extract and the hinokitiol stock solution so that the total concentration of the combined test formula of the plant extract and hinokitiol is 50 mg / mL. Without changing the total concentration, adjust the proportion of each component according to Table 2 to prepare the compositions in Table 2.

[0050] Test 1 Antibacterial Effect Test In this test, the minimum inhibitory concentration (MIC) of single plant extracts, hinokitiol, each plant extract prepared in Example 1, each composition prepared in Example 2, and OCT was determined by the microbroth dilution method to evaluate the inhibitory effect of each test drug on Malassezia; the synergistic antibacterial effect of each composition was evaluated by exploring the combination index (CI). The specific method is as follows.

[0051] 1. Test Materials Malassezia furfur (M. furfur, ATCC44344) was purchased from the Shanghai Culture Collection of Microorganisms, piroctone olamine (OCT) was purchased from Tongmeiyuan (Guangdong) Biotechnology Co., Ltd., Terminalia chebula fruits, Prunus mume fruits, Rosa rugosa flowers, Rosa chinensis flowers, and Tribulus terrestris fruits were purchased from Guangzhou Renshen Pharmaceutical Chain Co., Ltd.

[0052] 2. Test Methods (1) Preparation of modified Dxion (mDixon) medium and modified Dxion (mDixon) agar medium: Accurately weigh the medium granules, agar powder, and deionized water according to the instructions, place them in a beaker, stir with a glass rod until the granules are completely dissolved, adjust the pH value to 6.1 ± 0.1, dispense into conical flasks, seal, and sterilize at 121°C for 30 min. After cooling, store in a 4°C refrigerator for later use; (2) Preparation of bacterial suspension: Take out the glycerol tube of the test bacterium stored in a -20°C refrigerator, use an inoculation loop to take 20 μL of the bacterial solution, inoculate it on the agar slope by the streaking method, place it in a 32°C constant temperature incubator for 2 d, and rinse the slope with physiological saline to obtain a high-concentration bacterial suspension; take another test tube, pour in an appropriate amount of physiological saline, add an appropriate amount of the high-concentration bacterial suspension to make its turbidity consistent with the McFarland turbidity standard tube, that is, obtain a bacterial suspension with a final concentration of 1×10 6 CFU / mL; (3) Determination of MIC value: ① Culture method: Add 100 μL of modified Dixon (mDixon) liquid medium to each of the 2nd to 11th columns of a 96-well plate, and add 200 μL of modified Dixon (mDixon) liquid medium to the 12th column. Add 200 μL of each test drug to the 1st column, take 100 μL and dilute it two-fold horizontally to the 10th column (discard the extra 100 μL of the drug solution). Subsequently, add the bacterial suspension prepared in step (2) to the 1st to 11th columns, 100 μL for each well; at this time, the final volume of each well is 200 μL. The 11th column is the growth control well, and the 12th column is the blank control well. Place the 96-well plate in a constant temperature incubator at 32 °C for 1 - 2 days; ② Evaluation criteria: Observed with the naked eye, the concentration of the drug solution in the well without bacterial growth is the minimum inhibitory concentration (Minimum inhibitory concentration, MIC); (4) Synergistic antibacterial test and evaluation: ① The Chou-Talalay model is a commonly used pharmacological model for evaluating the synergistic and antagonistic effects of compound combinations during the onset process. Measure the MIC of each example according to the method described in (3), and calculate the synergistic effect index (CI) through the Chou-Talalay formula to determine whether there is synergy. The formula is as follows: ; ② Evaluation criteria: C a 、C b 、C c 、C d are the concentrations corresponding to the antibacterial effects when components A, B, C, and D act alone; C A 、C B 、C C 、C D represent the inhibitory effects observed at the corresponding concentrations when each component is formulated into a composition. The result of C A-D = the experimental data of the MIC of the composition × the proportion of the C A-D component in the composition; CI < 1 indicates the existence of a synergistic effect; CI = 1 indicates the absence of interaction; CI > 1 indicates the existence of an antagonistic effect.

[0053] 3. Test results (1) Table 3 shows the antibacterial results of single drugs.

[0054] Table 3 Antibacterial results of single drugs

[0055] As can be seen from Table 3, hinokitiol has excellent antibacterial effects, significantly better than the chemical anti-dandruff agent OCT, and its MIC is 1 / 2 of that of OCT; the plant extracts have good antibacterial effects, and their MICs are all < 50 mg / mL, but none of them are comparable to OCT.

[0056] (2) Tables 4 and 5 show the results of the synergistic antibacterial test of the plant extracts in Example 1.

[0057] Table 4 Synergistic Test Results of Plant Extracts I (mg / mL)

[0058] As can be seen from Table 4, when the weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract is 2 - 4:1, CI < 1, showing a synergistic antibacterial effect, and the MIC is 0.78125 mg / mL for both. The compounding of various plant extracts was carried out at a weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract of 2:1.

[0059] Table 5 Synergistic Test Results of Plant Extracts II (mg / mL)

[0060] As can be seen from Table 5, for Plant Extracts 5 - 10, it shows that as the content of Rosa rugosa extract increases, the MIC of the composition becomes smaller and the synergistic index CI becomes smaller, gradually changing from an antagonistic effect to a synergistic effect. The weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract is 2:1, and the weight ratio of Terminalia chebula fruit extract, Prunus mume fruit extract, Rosa rugosa extract and Tribulus terrestris fruit extract is 20:10:7 - 9:1 - 3, with CI < 1, showing a synergistic antibacterial effect.

[0061] For Plant Extracts 11 - 17, it shows that as the content of Rosa chinensis extract increases, the MIC of the composition becomes smaller and the synergistic index CI becomes smaller, gradually changing from an antagonistic effect to a synergistic effect. The synergistic effect of Plant Extract 17 is the strongest. The weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract is 2:1, and when the weight ratio of Terminalia chebula fruit extract, Prunus mume fruit extract, Rosa chinensis extract and Tribulus terrestris fruit extract is 20:10:7 - 9:1 - 3, CI < 1, showing a synergistic antibacterial effect.

[0062] Combined with the analysis of the above results, it can be seen that when the weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract is 2:1, and the weight ratio of Terminalia chebula fruit extract, Prunus mume fruit extract, flower extract (Rosa rugosa extract or Rosa chinensis extract) and Tribulus terrestris fruit extract is 20:10:7 - 9:1 - 3, CI < 1, showing a synergistic antibacterial effect.

[0063] Among them, the antibacterial effects of plant extract 9, plant extract 10, and plant extract 17 are better than those of the corresponding single plant extracts in Table 3; the rose extract is better than the Chinese rose extract in enhancing the synergistic antibacterial effect. Other tests and subsequent formulations with hinokitiol were carried out in the ratio of Terminalia chebula fruit extract: Prunus mume fruit extract: rose extract: Tribulus terrestris fruit extract = 20:10:7:3 (plant extract 8) and 20:10:10:0 (plant extract 10).

[0064] Table 6 shows the results of the synergistic antibacterial test of the composition of the plant extract and hinokitiol in Example 2.

[0065] Table 6 Synergistic test results of plant extract and hinokitiol (mg / mL)

[0066] As can be seen from Table 6, when the ratio of plant extract 8 to hinokitiol is 1 - 30:1, CI < 1, and the composition has a synergistic antibacterial effect. Moreover, when the ratio of plant extract 8 to hinokitiol is 1 - 3:1, the MIC of the composition is less than the MIC of the chemical antidandruff agent OCT.

[0067] When the ratio of plant extract 10 to hinokitiol is 1 - 30:1, CI < 1, and the composition has a synergistic antibacterial effect. Moreover, when the ratio of plant extract 10 to hinokitiol is 1 - 3:1, the MIC of the composition is less than the MIC of the chemical antidandruff agent OCT.

[0068] That is, when the ratio of the plant extract to hinokitiol is 1 - 30:1, it has a synergistic antibacterial effect. When the ratio of the plant extract to hinokitiol is 1 - 3:1, the MIC of the composition is less than the MIC of the chemical antidandruff agent OCT.

[0069] Test 2 Determination of the scavenging ability of DPPH free radicals This test evaluates the ability of each raw material to resist oxidative damage by measuring the scavenging ability of DPPH free radicals.

[0070] 1. Test materials: 1,1 - diphenyl - 2 - picrylhydrazyl (abbreviated as DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd.

[0071] 2. Test methods The samples and VC were prepared at concentrations of 20 mg / ml, 2 mg / ml, 200 μg / mL, 20 μg / mL, and 2 μg / mL. Using a pipette, 500 μL of the sample solutions at different concentrations were respectively taken and placed into plastic centrifuge tubes. Then, 500 μL of DPPH (0.2 mmol / L, prepared with ethanol) solution was added. After shaking well, it was left in the dark for 30 min. With ethanol as the blank control and VC as the positive control, the absorbance was measured at 517 nm, and three parallel experiments were conducted. According to the results of the preliminary experiment, the antioxidant activities of each raw material were compared at concentrations of 2 mg / ml and 200 μg / mL. The specific calculation formula is as follows: DPPH scavenging rate = (1 - D1 / D2) × 100%; Where: D1 is the absorbance of the test sample or positive control; D2 is the absorbance of the blank control.

[0072] 3. Test results (1) Table 7 shows the test results of the scavenging ability of some raw materials in Example 1 and Example 2 on DPPH free radicals.

[0073] Table 7 Test results of the scavenging ability of each raw material on DPPH free radicals

[0074] As can be seen from Table 7, the scavenging ability of hinokitiol alone on DPPH free radicals at 2 mg / ml and 200 μg / mL is less than that of OCT, and the antioxidant property of OCT is also relatively small; Plant extract 8 has excellent antioxidant properties, and its scavenging ability on DPPH free radicals at both 2 mg / ml and 200 μg / mL is comparable to that of VC, showing a highly significant difference from OCT; When the plant extract is compounded with hinokitiol, the antioxidant property of the composition can be significantly enhanced. The scavenging ability of Composition 3 on DPPH free radicals is significantly better than that of hinokitiol alone, and there is also a highly significant difference from OCT.

[0075] Therefore, the plant extract and composition provided by the present invention have significant antioxidant effects, can significantly enhance the antioxidant ability of hinokitiol, and are significantly better than OCT.

[0076] Test 3 Itching relief effect test This test evaluated the itching relief effects of each raw material through an itching mouse model.

[0077] 1. Test animals and reagents: SPF-grade, male BALB / c mice, weighing 18 - 22 g, animal certificate number: SCXK(Guangdong)2021 - 0041; all were purchased from the Experimental Animal Management Center of Southern Medical University. C48 / 80 was purchased from Shanghai Macklin Biochemical Co., Ltd.

[0078] 2. Test method: (1)Compound 48 / 80 is the pruritogen in this experimental itching model, and the subcutaneous injection dose for BALB / c mice on the back of the neck is 100 μg per mouse.

[0079] (2)Hair removal: Use a razor to remove the hair on the back of each BALB / c mouse, and the hair removal area is about 2 * 2 cm².

[0080] (3)Drug administration: Number the BALB / c mice, and subcutaneously inject a 100 μg / 50 μl compound C48 / 80 saline solution at the back of the neck. The injection dose is 50 μl. Immediately apply the corresponding raw materials to the hair-removed area on the back of the mice. Observe the number of times the mice lick their backs. The observation time is 20 min, and record the number of times the mice lick their backs within 20 min.

[0081] Blank control: Apply physiological saline to the hair-removed area on the back of the mice, and observe the number of times the mice lick their backs within 20 min.

[0082] (4)Statistical method The experiment uses a timer and a counter to simultaneously observe and record the number of times BALB / c mice lick their backs within 20 min after drug administration in each modeling group. The licking-back reaction is calculated as one lick when the BALB / c mouse turns its head to touch the hair-removed area and then turns back. When the licking duration exceeds 1 s, it is counted as two licks.

[0083] 3. Test results: (1)Table 8 shows the test results of the antipruritic effects of some raw materials in Example 1 and Example 2.

[0084] Table 8 Test results of the antipruritic effects of each raw material

[0085] As can be seen from Table 8, compared with the single use of OCT and hinokitiol, both plant extract 8 and composition 3 have better antipruritic effects.

[0086] Application Example 1 This application provides an anti-dandruff shampoo formula with antibacterial, antioxidant, and antipruritic triple effects. The antibacterial (anti-dandruff and antipruritic ingredients) is composition 3, and composition 3 is named Baicuisu Jing.

[0087] (1)Baicuisu Jing shampoo formula The weight parts of each ingredient of the shampoo are shown in Table 9, and its preparation method includes the following steps: S1. Add the A1-phase water to the main pot, add the remaining raw materials of the A1 phase under homogenization, disperse completely, add the A2-phase raw materials, stir well, heat to 80 - 85 °C, and add the A3-phase raw materials and stir until completely dissolved; S2. Add the B1 phase, stir until completely dissolved, and then add the B2 phase and stir until completely dissolved; S3 cool to 45`50 ℃, sequentially add phase C raw materials, stir until transparent; S4. Sequentially add the D phase raw materials, stir and cool to room temperature; S5. Take samples for testing, and discharge the materials if they pass the test.

[0088] Table 9: Formula of Baicui Clean Shampoo

[0089] (2) Volunteer test of Biorefreshing Shampoo Thirty male and female volunteers aged 18 to 50 were recruited (eligible volunteers were healthy individuals with oily hair, dandruff, or itchy scalp). They used the shampoo every other day (every other day) for four weeks and were evaluated on three aspects: hair volume, dandruff removal, and itching relief. Each aspect was scored on a scale of 0 to 7 (7 = extremely satisfied, 6 = very satisfied, 5 = somewhat satisfied, 4 = average, 3 = somewhat satisfied, 2 = very dissatisfied, and 1 = extremely dissatisfied). Higher levels of approval were associated with higher scores. Overall satisfaction included those who scored 5 to 7. Approval = overall satisfaction divided by total number of volunteers. The volunteer test results are shown in Table 10.

[0090] Table 10 Test results of Baicuisu pure shampoo

[0091] As shown in Table 10, Baicui Sujing Shampoo has good hair volume, dandruff removal and itching relief effects.

[0092] Application Example 2 This application provides an antipruritic solution with triple effects of antibacterial, antioxidant, and antipruritic. Composition 3 (Baicuisujing) was selected for formulation proofing.

[0093] (1) Formula of Baicuisujing Antipruritic Liquid The weight parts of the components of the antipruritic solution are shown in Table 11, and the preparation method thereof comprises the following steps: S1. Mix and dissolve phase A, stir evenly and set aside; S2. Mix and dissolve phase B, stir evenly, add phase B to phase A, and stir evenly; S3. Take samples to test pH, and discharge only those that pass the test.

[0094] Table 11: Formula of Baicuisujing Antipruritic Liquid

[0095] (2) Volunteer test of Baicuisujing anti-itching liquid Fifteen male and female volunteers aged 18 to 50 were recruited. 0.1% histamine was applied to the arms of the volunteers using a cotton swab. After 15 minutes, symptoms such as wheals, itching, and papules appeared on the volunteers' arms. The effects of the solution were then applied to the volunteers' arms, and the results were recorded over a 20-minute period. The volunteers were scored based on both itching relief and redness (wheal) reduction. Each aspect was scored on a scale of 0 to 7 (7 for extremely satisfied, 6 for very satisfied, 5 for somewhat satisfied, 4 for average, 3 for somewhat dissatisfied, 2 for very dissatisfied, and 1 for extremely dissatisfied). The higher the level of approval, the higher the score. Overall satisfaction includes those who scored 5 to 7, and approval = overall satisfaction divided by total number of participants. The volunteer test results are shown in Table 12.

[0096] Table 12 Test results of Baicuisujing antipruritic liquid

[0097] As shown in Table 12, Baicuisujing Antipruritic Liquid has good recognition in relieving itching and removing redness (wheals).

[0098] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant extract having the triple effects of antibacterial, antioxidant and antipruritic, characterized in that: The plant extract includes a and b; aIncluding Terminalia chebula fruit extract; b is plum fruit extract, or at least one of rose flower extract, rose flower extract or tribulus terrestris fruit extract and plum fruit extract; When b is plum fruit extract, the weight ratio of a to b is 2-4:1; When b is at least one of rose flower extract, rose flower extract or tribulus terrestris fruit extract and plum fruit extract, the weight ratio of a to b is 1:

1.

2. The plant extract according to claim 1, characterized in that The b is plum fruit extract.

3. The plant extract according to claim 1, characterized in that Said b is plum fruit extract and flower extract; The weight ratio of the plum fruit extract to the flower extract is 1:1; the flower extract is rose flower extract or rose flower extract.

4. The plant extract according to claim 1, characterized in that Said b is plum fruit extract, flower extract and tribulus terrestris fruit extract; The weight ratio of the plum fruit extract, the flower extract and the tribulus terrestris fruit extract is 10:7-9:1-3; the flower extract includes rose flower extract or rose flower extract.

5. The method for preparing the plant extract according to any one of claims 1 to 4, characterized in that: The method includes mixing and soaking the plant raw materials of each component and the extractant in a volume ratio of 1:8-12, ultrasonicating, concentrating the filtrate, centrifuging and taking the supernatant to obtain a single plant extract, and mixing according to a formula to obtain the plant extract.

6. A composition having the triple effects of antibacterial, antioxidant and antipruritic, characterized in that: Comprising the plant extract according to any one of claims 1 to 4 or the plant extract prepared by the preparation method according to claim 5, and hinokitiol; The weight ratio of the plant extract to hinokitiol is 1-30:

1.

7. The composition according to claim 6, characterized in that The weight ratio of the plant extract to hinokitiol is 1-3:

1.

8. The method for preparing the composition according to claim 6 or 7, characterized in that: The method comprises mixing a hinokitiol solution with a plant extract according to a recipe.

9. The preparation method according to claim 8, characterized in that The concentration of hinokitiol in the hinokitiol alcohol solution is 1-200 mg / mL.

10. Use of the plant extract according to any one of claims 1 to 4, the plant extract obtained by the preparation method according to claim 5, the composition according to claim 6 or 7, or the composition obtained by the preparation method according to claim 8 or 9 in the preparation of a scalp care product or a hair care product.

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