Preparation method of collagen peptide liposome with stable sodium alginate and photoprotection activity

Abstract

The invention belongs to the technical field of liposome modification, and particularly relates to a method for modifying stable light protection active collagen peptide liposome by adopting sodium alginate. Comprising the following steps: (1) adding a photoprotection active collagen peptide solution into a lipid film for hydration, and homogenizing to obtain a liposome suspension; and (2) dropwise adding a sodium alginate aqueous solution into the liposome suspension obtained in the step (1), mixing and stirring, and standing to obtain the photo-protection active collagen peptide liposome. The liposome prepared by the invention has excellent slow release performance, storage stability and gastrointestinal tract digestion stability, can stably release light in vivo for a long time to protect active collagen peptide, and can play a role in light protection for a long time; meanwhile, the preparation method is green and environment-friendly, the stability and bioavailability of the photoprotection active collagen peptide are greatly improved, and a brand new technical direction is opened up for research and development of cosmetics and medical products in the field of photoprotection.

Description

Technical Field

[0001] The invention belongs to the technical field of liposome modification, and particularly relates to a method for modifying and stabilizing photoprotective active collagen peptide liposomes by using sodium alginate. Background Art

[0002] According to the source of the influencing factors, skin aging can be divided into natural aging and photoaging. Natural aging is irreversible, so photoaging is the current focus of the prevention and treatment of skin aging, and there are corresponding physical and chemical prevention and treatment methods. However, traditional photoprotective agents such as chemical sunscreens have potential skin irritation and environmental toxicity problems, while physical sun protection methods such as parasols and sun hats have limited protective effects and are inconvenient to use. Photoprotective active collagen peptides have become a research hotspot in the field of photoprotection due to their high-efficiency antioxidant, anti-photodamage and cell repair properties. However, photoprotective active collagen peptides themselves have poor stability, are easily degraded by enzymes, and have low bioavailability in the body. It is difficult to act accurately and efficiently on the parts damaged by light, which greatly limits their actual application effect.

[0003] Liposomes, as widely used drug carriers, possess a unique phospholipid bilayer structure that can effectively encapsulate both hydrophilic and hydrophobic drugs, providing physical protection and preventing premature degradation. By regulating the particle size, surface charge, and composition of liposomes, targeted drug delivery can be achieved, increasing drug concentration at the site of disease and reducing toxic side effects on normal tissues. However, conventional liposomes have drawbacks in photoprotection, including a short blood circulation half-life and easy clearance, a single surface property, and poor targeting, requiring modification and optimization to meet the requirements.

[0004] Sodium alginate is a natural polysaccharide that is biocompatible, degradable, and low in immunogenicity. Its carboxyl groups can form complexes with cations, crosslink metal ions to create sustained-release formulations, and regulate drug release. Based on these properties, its use in modifying photoprotective collagen peptide liposomes offers significant advantages, including enhanced stability, reduced aggregation, prolonged circulation, and unique surface properties, improving targeting and affinity for photodamaged tissues. This provides new insights into the innovative development of photoprotection technologies. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for preparing sodium alginate-stabilized photoprotective active collagen peptide liposomes.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for preparing sodium alginate-stabilized photoprotective active collagen peptide liposomes, comprising the following steps:

[0008] (1) adding a photoprotective active collagen peptide solution to a lipid film for hydration and homogenization to obtain a liposome suspension;

[0009] (2) adding the sodium alginate aqueous solution dropwise to the liposome suspension obtained in step (1), mixing and stirring, and allowing to stand to obtain photoprotective active collagen peptide liposomes;

[0010] The photoprotective polypeptide is any one or more of the following: GPPSGGFG, FPGYPF, FPPQFP, WVGF, FFPNDP, GPPSGGF, GPLGPP, SGGFDW, FGGGY, TFGF, FAVF, LGGFP, FGYP, GPPGPP, GPPGPGP, FDGL, FGPY, LAGF, GPPGGP, GYSW, QPPGP, FQGF, FDDF, LFGGP, FTDF, SPLFA, FGSF, FLGGP, FLNF, FAGPP, SPLF, PGPP, PFDGL, SPLGF, GPKLGF, NPPGP, FGYL, GFGF, SGGFG, GYDF, VGPF, FGGF, SGGF, SPAFP, FSGL, FRDF, AGFG, FGGP, GPGPP, FADF, SPFP (as shown in SEQ ID NOs 1 to 51).

[0011] Preferably, the method for preparing the lipid film in step (1) is: mixing cholesterol, lecithin, Tween and anhydrous ethanol, stirring, and evaporating to dryness to obtain the lipid film.

[0012] Preferably, the volume ratio of cholesterol, lecithin, Tween and anhydrous ethanol is: 2.5-20:1:1:50-100.

[0013] Preferably, the concentration of the photoprotective active collagen peptide solution in step (1) is 0.5 to 1.5 mg / mL.

[0014] Preferably, the mass fraction of the sodium alginate aqueous solution in step (2) is 0.10% to 0.90%.

[0015] Preferably, the dripping speed in step (2) is 15 to 25 drops / min; and the volume ratio of the sodium alginate aqueous solution to the liposome suspension is 1:1.

[0016] Preferably, the stirring speed in step (2) is 70-90 rpm, and the stirring time is 50-70 min; the ambient temperature of the standing state is 3-5° C., and the stirring time is 1.5-2.5 h.

[0017] The present invention also provides sodium alginate-stabilized light-protection active collagen peptide liposomes prepared by the preparation method.

[0018] Preferably, the particle size of the sodium alginate-stabilized photoprotective active collagen peptide liposome is 118.14 to 243.44 nm.

[0019] The present invention also provides the use of the liposome in preparing health-care food or cosmetics for light protection or light damage repair.

[0020] The present invention has the following beneficial effects:

[0021] (1) Sodium alginate is a natural polysaccharide with negative charge and good biocompatibility. It can interact with the surface of liposomes through electrostatic interaction, preventing the aggregation of liposomes due to van der Waals forces, thereby effectively improving the stability of liposomes. Specifically, after storage at 4°C for 28 days, the embedding rate of liposomes modified with sodium alginate was above 60.56±3.28%, which was only 10.38% lower than that on the first day, and the particle size was stable at 186.06±4.83nm. The embedding rate of liposomes not modified with sodium alginate dropped to 52.55±1.03% after 28 days, and the particle size expanded to 239.69±12.40nm. After storage at 25°C for 28 days, the entrapment efficiency of the liposomes decreased to 37.08±1.25% and the particle size expanded to 351.72±7.53, while the entrapment efficiency of the alginate-modified liposomes remained stable at 47.01±1.81% and the particle size was 279.59±8.78%.

[0022] (2) The peptide described in the present invention is a hydrophobic peptide with a strong bitter taste. Using liposomes to embed the peptide can reduce its bitterness and enhance its dispersibility in aqueous systems, allowing the photoprotective active collagen peptide to play a role in a variety of application scenarios.

[0023] (3) The Sa-Lip-Pep provided by the present invention has excellent sustained-release storage and gastrointestinal digestion stability. After 24 hours of in vitro release testing, the release rate of Sa-Lip-Pep was only 63.32%, which was 14.41% lower than the 77.73% release rate of liposomes. After gastrointestinal digestion, the release rate of sodium alginate was 61.88%, while the release rate of liposomes reached 81.64%. After storage at 25°C and 4°C for 28 days, Sa-Lip-Pep's entrapment efficiency, particle size, and Zeta potential were all better than those of liposomes, and it had good stability.

[0024] (4) The present invention uses natural phospholipids, cholesterol and sodium alginate as key raw materials, among which the modification of sodium alginate is a major innovative highlight. The present invention first constructs the basic structure of liposomes, and then uses sodium alginate to modify their surface. This modification not only optimizes the particle size and membrane structure of the liposomes, so that the liposomes have excellent sustained-release performance, storage and gastrointestinal digestion stability, and can release photoprotective active collagen peptides in the body for a long time and stably, exerting a lasting photoprotective effect; and the good biocompatibility of sodium alginate further enhances the overall safety. At the same time, this preparation method is green and environmentally friendly, greatly improving the stability and bioavailability of photoprotective active collagen peptides, and opening up a new technical direction for the research and development of cosmetics and pharmaceutical products in the field of photoprotection. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to make the objectives, technical solutions and advantages of the embodiments of the present invention easier to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0026] Figure 1 The effect of the mass ratio of lecithin to cholesterol on the encapsulation efficiency and particle size of Lip-Pep;

[0027] Figure 2 The effect of the mass ratio of lecithin and Tween on the encapsulation efficiency and particle size of Lip-Pep;

[0028] Figure 3 is the effect of peptide concentration on Lip-Pep entrapment efficiency and particle size;

[0029] Figure 4 Fourier transform infrared spectra of peptide (Pep), liposome (Lip), liposome-embedded peptide (Lip-Pep), sodium alginate (Sa), and sodium alginate-modified liposome-embedded peptide (Sa-Lip-Pep);

[0030] Figure 5 The changes in the entrapment efficiency of Lip-Pep and Sa-Lip-Pep stored at 4°C and 25°C for 28 days;

[0031] Figure 6 The particle size changes of Lip-Pep and Sa-Lip-Pep after storage at 4°C and 25°C for 28 days;

[0032] Figure 7 The zeta potential changes of Lip-Pep and Sa-Lip-Pep after storage at 4°C and 25°C for 28 days;

[0033] Figure 8 The changes in peptide release rates during gastrointestinal digestion of Lip-Pep and Sa-Lip-Pep;

[0034] Figure 9 Particle size changes of Lip-Pep and Sa-Lip-Pep during gastrointestinal digestion

[0035] Figure 10 The sustained release performance of Pep, Lip-Pep and Sa-Lip-Pep. Specific implementation methods

[0036] The following description sets forth numerous specific details to facilitate a thorough understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein, and those skilled in the art may make similar generalizations without departing from the scope of the present invention. In the following examples, unless otherwise noted, the experimental methods used are conventional methods, and the materials and reagents used can be purchased from biological or chemical reagent companies.

[0037] Example 1

[0038] The present invention intends to prepare photoprotective active collagen peptide liposomes using photoprotective active collagen peptide as raw material through a thin film dispersion method. After single-factor experiments, the mass ratio of lecithin to cholesterol, the mass ratio of lecithin to Tween, and the concentration of photoprotective active collagen peptide have a great influence on the encapsulation efficiency and are identified as design variables. With the encapsulation efficiency and particle size as response values, the response surface analysis method is used to determine the optimal process for preparing photoprotective active collagen peptide liposomes.

[0039] (1) Preparation of photoprotective collagen peptide liposomes: lecithin, cholesterol, Tween, and photoprotective collagen peptide (GPPSGGFG) were accurately weighed in a certain mass ratio, dissolved in 10 mL of anhydrous ethanol by ultrasonication, and then rotary evaporated at 45°C and 0.05 MPa for 30 min. The mixture was then placed in a desiccating dish overnight.

[0040] (2) preparing a sodium alginate solution;

[0041] The sodium alginate aqueous solution was added dropwise to the collagen peptide liposome suspension at a rate of 20 drops per minute. The two liquids were mixed and stirred at 80 rpm at room temperature in a volume ratio of 1:1 for 1 hour, and then allowed to stand at 4°C for 2 hours to obtain sodium alginate-stabilized photoprotective active collagen peptide liposomes.

[0042] (3) Single factor experiments were conducted using the following four factors to study their effects on liposome encapsulation efficiency, particle size, PDI, and loading capacity. Specific examples are as follows:

[0043] The mass ratio of lecithin to cholesterol was 2.5:1, and other factors were fixed: the mass concentration of Tween was 2 mg / mL, and the mass concentration of photoprotective active collagen peptide was 1 mg / mL.

[0044] Example 2

[0045] The difference from Example 1 is that the mass ratio of lecithin to cholesterol is 5:1.

[0046] Example 3

[0047] The difference from Example 1 is that the mass ratio of lecithin to cholesterol is 10:1.

[0048] Example 4

[0049] The difference from Example 1 is that the mass ratio of lecithin to cholesterol is 15:1.

[0050] Example 5

[0051] The difference from Example 1 is that the mass ratio of lecithin to cholesterol is 20:1.

[0052] The entrapment rate refers to the ratio of the amount of drug embedded in the liposome to the total amount of drug in the liposome (including embedded and unentrapped drugs). It is an important indicator for evaluating the quality of liposomes.

[0053] The sodium alginate-modified peptide liposomes prepared in Examples 1-5 were collected and the particle size, PDI, and zeta potential were measured using a nano-laser particle size analyzer. Three replicates were performed for each sample, and the three replicates were averaged. The entrapment efficiency was then determined using the following calculation method:

[0054]

[0055] The embedding rate and particle size of the liposomes obtained in Examples 1-5 were obtained. Figure 1 ,Depend on Figure 1 It can be seen that with the increase of the mass ratio of lecithin to cholesterol, the encapsulation rate of the photoprotective active collagen peptide liposomes first increases and then decreases, and the particle size first decreases and then increases. When the mass ratio of lecithin to cholesterol is 10:1, the encapsulation rate reaches the maximum, which is 74.99±2.93%, and the particle size reaches the minimum, which is 101.84±0.92nm.

[0056] Example 6

[0057] Based on the preparation method of Example 1, the mass ratio of lecithin to Tween was 2.5:1, and other factors were kept constant: the mass concentration of cholesterol was 2 mg / mL, and the mass concentration of photoprotective active collagen peptide was 1 mg / mL.

[0058] Example 7

[0059] The difference from Example 6 is that the mass ratio of lecithin to Tween is 5:1.

[0060] Example 8

[0061] The difference from Example 6 is that the mass ratio of lecithin to Tween is 10:1.

[0062] Example 9

[0063] The difference from Example 6 is that the mass ratio of lecithin to Tween is 15:1.

[0064] Example 10

[0065] The difference from Example 6 is that the mass ratio of lecithin to Tween is 20:1.

[0066] The embedding rate and particle size of the liposomes obtained in Examples 6-10 were obtained. Figure 2 ,Depend on Figure 2 It can be seen that when the mass ratio of lecithin to Tween is 10:1, the liposome encapsulation efficiency reaches the maximum and the particle size is the lowest.

[0067] Example 11

[0068] Based on the preparation method of Example 1, the mass concentration of the photoprotective active collagen peptide was 0.5 mg / mL, and other factors were fixed: the mass ratio of lecithin to Tween was 10:1, the mass concentration of cholesterol was 2 mg / mL, and the mass concentration of Tween was 2 mg / mL.

[0069] Example 12

[0070] The difference from Example 11 is that the mass concentration of the photoprotective active collagen peptide is 0.75 mg / mL.

[0071] Example 13

[0072] The difference from Example 11 is that the mass concentration of the photoprotective active collagen peptide is 1.00 mg / mL.

[0073] Example 14

[0074] The difference from Example 11 is that the mass concentration of the photoprotective active collagen peptide is 1.25 mg / mL.

[0075] Example 15

[0076] The difference from Example 11 is that the mass concentration of the photoprotective active collagen peptide is 1.50 mg / mL.

[0077] The embedding rate and particle size of the liposomes obtained in Examples 11-15 were obtained. Figure 3 ,Depend on Figure 3 It can be seen that when the mass concentration of the photoprotective active collagen peptide is 1.00 mg / mL, the liposome encapsulation rate reaches the maximum and the particle size is the lowest.

[0078] According to the optimization results of DesignExpert software, the optimal process conditions for the preparation of photoprotective active collagen peptide liposomes were predicted by response surface optimization experiments as follows:

[0079] The predicted optimal conditions for preparing photoprotective collagen peptide liposomes were 10.24:1 (mass ratio of lecithin to cholesterol, 9.85:1 (mass ratio of lecithin to Tween), and 0.93 mg / mL (peptide concentration). The particle size was 114.36 ± 8.87 nm, with an error of 4.11% from the predicted value, which was close to the model. This indicates that the response surface fit results were good.

[0080] Example 16

[0081] Based on the preparation method of Example 1, a sodium alginate solution with a mass fraction of 0.1% was prepared. The sodium alginate aqueous solution was added dropwise to the collagen peptide liposome suspension at a rate of 20 drops per minute. The two liquids were mixed and stirred at 80 rpm at a volume ratio of 1:1 at room temperature for 1 hour, and then placed at 4°C for 2 hours.

[0082] Example 17

[0083] The difference from Example 16 is that the mass fraction of sodium alginate is 0.3%.

[0084] Example 18

[0085] The difference from Example 16 is that the mass fraction of sodium alginate is 0.5%.

[0086] Example 19

[0087] The difference from Example 16 is that the mass fraction of sodium alginate is 0.7%.

[0088] Example 20

[0089] The difference from Example 16 is that the mass fraction of sodium alginate is 0.9%.

[0090] The embedding efficiency, particle size, zeta potential and PDI corresponding to the liposomes obtained in Examples 16-20 are shown in Table 1. As can be seen from Table 1, the particle size and embedding efficiency of the liposomes continue to increase with the increase of the mass fraction of sodium alginate. When the concentration of sodium alginate increases from 0.1% to 0.9%, the particle size increases from 118.14nm to 243.44nm. This may be because the sodium alginate molecules are adsorbed on the surface of the liposomes, thereby increasing the effective size of the liposomes and causing the particle size to increase. However, only the embedding efficiency of 0.1% and 0.9% showed a significant difference (P < 0.05), which shows that sodium alginate has little effect on the embedding efficiency of photoprotective active collagen peptides. The absolute values of the zeta potential at the five concentrations are all greater than 25, indicating that the formed system has strong stability.

[0091] Overall, the PDI first decreases and then increases with increasing sodium alginate concentration. Within a certain range, sodium alginate makes the interactions between liposomes more uniform, narrowing the liposome size distribution and reducing the PDI. However, excessive alginate concentrations can lead to excessive liposome aggregation, resulting in an uneven particle size distribution and an increase in the PDI. For these reasons, a 0.5% sodium alginate concentration was selected as the outer liposome embedding layer in subsequent experiments.

[0092] Table 1. Sodium alginate with different mass fractions

[0093]

[0094] 1. Liposome stability analysis

[0095] FTIR was used to evaluate the modification effect, and collagenase inhibition rate was used to study the changes in the photoprotective activity of the photoprotective active collagen peptide liposomes before and after modification. The stability of the photoprotective active collagen peptide liposomes before and after modification was then analyzed in terms of storage stability, ionic strength stability, and thermal stability.

[0096] 2. Fourier transform infrared spectroscopy (FTIR) analysis

[0097] The nanoliposomes were dried by freeze drying, ground into powder and mixed evenly with 1% potassium bromide powder, added to the pressed film to make a transparent sample slice, and then the slice was placed in the instrument beam for measurement. Figure 4 , proving that the photoprotective active collagen peptide was successfully encapsulated into the liposomes and sodium alginate was successfully encapsulated in the outer layer of the liposomes. When the empty liposomes were loaded with peptides, the absorption peak vibration (CO expansion vibration) of the liposomes changed from 864 cm -1 Transfer to 858cm -1 , which indicates that the peptide and liposome may have produced hydrogen bonding. - The symmetrical stretching of 1244cm -1 Transfer to 1251cm -1 The loading of peptide resulted in the peak shift from 3321 cm -1 Moves (in empty liposomes) to 3354 cm -1 , which can be attributed to the formation of hydrogen bonds between OH and NH groups. After sodium alginate modified liposomes, the liposomes showed a -1 The absorption peak at 1741.75 (C=O) disappears, indicating that sodium alginate is tightly wrapped on the surface of liposomes. -1 The movement of the liposomes indicated the formation of hydrogen bonds between the liposomes and sodium alginate.

[0098] 3. Liposome stability determination

[0099] (1) Storage stability

[0100] The sodium alginate modified liposomes and liposomes were stored in the dark at 4 and 25 °C for 28 days, and the stability of the liposomes was evaluated by three indicators: entrapment rate, particle size, and potential. Figure 5 、 6 , 7. With the extension of storage time, the entrapment efficiency of both liposomes gradually decreased, the particle size increased, and the system stability decreased, which may be due to rupture and leakage. However, the entrapment efficiency and particle size of the liposomes modified with sodium alginate changed relatively little. After storage at 4°C for 28 days, the entrapment efficiency of the liposomes modified with sodium alginate was above 60.56±3.28%, which was only 10.38% lower than that on the first day, and the particle size was stable at 186.06±4.83nm. In contrast, the entrapment efficiency of the liposomes without sodium alginate modification decreased to 52.55±1.03% after 28 days, and the particle size expanded to 239.69±12.40nm. After 28 days of storage at 25°C, the entrapment efficiency of the liposomes decreased to 37.08±1.25%, and the particle size increased to 351.72±7.53. In contrast, the entrapment efficiency of the alginate-modified liposomes remained stable at 47.01±1.81%, and the particle size was 279.59±8.78%. After 28 days of storage at 4°C and 25°C, the zeta potential of Sa-Lip-Pep increased to -40.40±1.48 mV and -34.42±4.16 mV, respectively, compared to -32.72±1.42 mV and -25.93±1.68 mV for the unmodified liposomes, respectively. The zeta potential of both liposomes increased, but the absolute values remained above 25 mV, indicating that both systems maintained good stability during storage. Furthermore, the absolute values of the zeta potential of Sa-Lip-Pep were higher than those of the liposomes, indicating that alginate modification enhances liposome stability. Comparing the effects of temperature on liposomes, we found that both liposomes were more stable at 4°C than at 25°C. This is likely because higher temperatures increase the likelihood of collision and aggregation between sample particles, thereby disrupting the structure of the encapsulated system. Overall, 4°C is a more suitable storage temperature for liposomes.

[0101] (2) Gastrointestinal digestion stability

[0102] The changes of the two liposomes before and after simulated gastrointestinal digestion were evaluated by peptide release rate and particle size. Figure 7 and Figure 8. After 2 hours of simulated gastric juice digestion, the release rates of Sa-Lip-Pep and Lip-Pep were 33.12±0.65% and 41.76±0.65%, respectively. This phenomenon indicates that the modification of sodium alginate improves the gastric digestion stability of peptide liposomes. In addition, after 2 hours of simulated gastric juice digestion, the particle size of Lip-Pep increased significantly, reaching 331.63±3.17nm, which may be due to the acidic conditions reducing the surface charge of the Lip-Pep system and promoting the aggregation between liposomes. In contrast, the particle size of Sa-Lip-Pep increased to 288.80±5.03nm after 2 hours of gastric digestion. Its better stability is due to the electrostatic interaction between sodium alginate and liposomes and the steric hindrance effect, which reduces the aggregation between liposomes. After 4 hours of simulated intestinal digestion, the release rates of Sa-Lip-Pep and Lip-Pep were 61.88±4.67% and 80.26±4.67%, respectively. The particle sizes also increased to 513.58±6.51nm and 401.65±0.58nm, respectively. This is likely due to the combined effects of micelles, bile salts, and a large number of anions in the simulated intestinal fluid. These findings suggest that sodium alginate modification helps prevent the leakage and degradation of photoprotective active collagen peptides, enhancing the activity and stability of Lip-Pep during gastrointestinal digestion.

[0103] (3) Collagenase inhibitory activity

[0104] Table 2 Collagenase inhibition rate of different photoprotective active collagen peptide liposomes

[0105]

[0106] As shown in Table 2, although the collagenase inhibition rate of the peptide decreased after liposome encapsulation, the overall difference was not significant. Furthermore, further encapsulation of the liposomes with sodium alginate did not significantly affect the collagenase inhibition rate. Therefore, encapsulation with liposomes and sodium alginate can enhance the stability of the peptide while maintaining its photoprotective effect.

[0107] 4. Liposome sustained-release performance

[0108] In order to effectively deliver the photoprotective active collagen peptides to the skin and the corresponding target sites, the release of the embedded materials should remain constant for a long time in vitro. Therefore, the release behavior of Sa-Lip-Pep and Lip-Pep was evaluated using the dialysis bag diffusion technique. The results of the sustained release assay are shown in Figure 2. Figure 10As shown, the unencapsulated peptide released most rapidly in PBS, reaching a cumulative release rate of 83.56±2.83% after 6 hours. After 10 hours, the cumulative release rates of Sa-Lip-Pep and Lip-Pep reached 59.68±0.38% and 69.57±0.02%, respectively. After 14 hours, the increase in the cumulative release rate of the peptide slowed down. At 24 hours, the encapsulation efficiencies of the unencapsulated peptide, Sa-Lip-Pep, and Lip-Pep were 95.56±2.40%, 63.33±1.23%, and 77.73±0.30%, respectively. Liposome encapsulation effectively enhanced the sustained release of the peptide, which was further enhanced by sodium alginate modification. The excellent sustained release performance of Sa-Lip-Pep may be due to the fact that, in addition to electrostatic and steric effects, the sodium alginate attracts water molecules on the liposome exterior, forming a hydration layer that reduces peptide leakage.

[0109] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A method for preparing sodium alginate-stabilized photoprotective active collagen peptide liposomes, characterized in that: The steps include: (1) adding a photoprotective active collagen peptide solution to a lipid film for hydration and homogenization to obtain a liposome suspension; (2) adding the sodium alginate aqueous solution dropwise to the liposome suspension obtained in step (1), mixing and stirring, and allowing to stand to obtain photoprotective active collagen peptide liposomes; The photoprotective polypeptide is any one or more of the following: GPPSGGFG, FPGYPF, FPPQFP, WVGF, FFPNDP, GPPSGGF, GPLGPP, SGGFDW, FGGGY, TFGF, FAVF, LGGFP, FGYP, GPPGPP, GPPGPGP, FDGL, FGPY, LAGF, GPPGGP, GYSW, QPPGP, FQGF, FDDF, LFGGP, FTDF, SPLFA, FGSF, FLGGP, FLNF, FAGPP, SPLF, PGPP, PFDGL, SPLGF, GPKLGF, NPPGP, FGYL, GFGF, SGGFG, GYDF, VGPF, FGGF, SGGF, SPAFP, FSGL, FRDF, AGFG, FGGP, GPGPP, FADF, SPFP.

2. The preparation method according to claim 1, characterized in that The preparation method of the lipid film in step (1) is as follows: cholesterol, lecithin, Tween and anhydrous ethanol are mixed and stirred, and evaporated to dryness to obtain the lipid film.

3. The preparation method according to claim 2, characterized in that The volume ratio of the cholesterol, lecithin, Tween and anhydrous ethanol is: 2.5-20:1:1:50-100.

4. The preparation method according to claim 1, characterized in that The concentration of the photoprotective active collagen peptide solution in step (1) is 0.5 to 1.5 mg / mL.

5. The preparation method according to claim 1, characterized in that The mass fraction of the sodium alginate aqueous solution in step (2) is 0.10% to 0.90%.

6. The preparation method according to claim 1, characterized in that The dripping speed in step (2) is 15 to 25 drops / min; the volume ratio of the sodium alginate aqueous solution to the liposome suspension is 1:

1.

7. The preparation method according to claim 1, characterized in that The stirring speed in step (2) is 70-90 rpm, and the stirring time is 50-70 min; the ambient temperature of the standing state is 3-5° C., and the stirring time is 1.5-2.5 h.

8. Sodium alginate-stabilized photoprotective active collagen peptide liposomes prepared by the preparation method according to any one of claims 1 to 7.

9. The sodium alginate-stabilized photoprotective active collagen peptide liposome according to claim 8, characterized in that: The particle size of the sodium alginate-stabilized light-protective active collagen peptide liposome is 118.14-243.44 nm.

10. Use of the liposome according to claim 8 or 9 in the preparation of health-care foods or cosmetics for light protection or light damage repair.

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