A composition with anti-wrinkle and firming effects and its application

Abstract

This invention provides a composition with anti-wrinkle and firming effects and its application. The composition includes sterols, niacinamide, and arginine / lysine peptides; the sterols include PEG-10 soybean sterol. This invention creatively combines niacinamide, arginine / lysine peptides, and sterols into a novel composition. These three components exhibit excellent anti-wrinkle and firming effects at low concentrations and have potential synergistic effects. In particular, PEG-10 soybean sterol can enhance the synergistic anti-wrinkle and firming effects of niacinamide and arginine / lysine peptides. All three components improve skin elasticity and firmness by promoting type I collagen gene expression, elastin expression, enhancing antioxidant capacity, and increasing elastase inhibition rate.

Description

Technical Field

[0001] This invention belongs to the field of daily cosmetics technology, specifically relating to a composition with anti-wrinkle and firming effects and its application. Background Technology

[0002] The global anti-aging market reached $266.2 billion in 2024 and is projected to surge to nearly $500 billion by 2032, with a compound annual growth rate of 8%. In 2023, the Chinese anti-aging skincare market exceeded 150 billion yuan, accounting for 62% of the industry share. Skincare products hold a dominant 58% share of the anti-aging market. However, consumers' understanding of anti-aging has long surpassed the ordinary needs of "anti-wrinkle" and "firming," shifting towards a deeper integration of cosmetics and medical aesthetics, as well as the superposition of innovative technologies. For example, non-invasive cosmetic effects and innovative methods such as nano-transdermal technology can significantly improve the absorption rate of active ingredients, which are issues that consumers value highly.

[0003] CN119280102A discloses a skincare composition with anti-aging effects, its preparation method, and its application, belonging to the field of beauty and skincare technology. In terms of ingredient combination, retinol, polypeptide complex, ceramide, astaxanthin, and resveratrol work synergistically to comprehensively combat aging. Each ingredient individually promotes collagen production, addresses muscle contraction wrinkles, enhances barrier function, scavenges free radicals, and inhibits melanin formation. In terms of preparation method, advanced technologies such as precise temperature control, suitable stirring methods, and high-precision mixers ensure uniform mixing of ingredients. Inert gas protection and a PID temperature controller preserve the activity and efficacy of the ingredients. In terms of application, it can be rationally added to face creams, lotions, serums, and masks to meet diverse needs, providing deep nourishment, refreshing care, efficient penetration, and immediate effects, respectively. In short, it provides consumers with an efficient, safe, and comprehensive anti-aging solution, improving signs of aging, with broad prospects and high value.

[0004] CN116763683A discloses a skin anti-aging composition and its application. The main raw materials include: solvent, moisturizer, oxidized tannic acid, oxidized linoleic acid, skin conditioning agent, antioxidant, thickener, solubilizer, and fragrance. This invention optimizes the formulation of the skin anti-aging composition by precisely combining multiple active ingredients to synergistically combat aging, fully leveraging the skincare effects of each component. The addition of multiple effective ingredients enhances the anti-aging effect, achieving a significant increase in skincare efficacy. This invention introduces oxidized tannic acid and oxidized linoleic acid into the preparation of the skin anti-aging composition, allowing them to interact with another component, collagen, to form a stable network, thereby improving the composition's antioxidant activity and emulsification stability.

[0005] While the anti-aging products with different mechanisms of action mentioned above can improve skin laxity and other issues to some extent, their active ingredients generally suffer from poor stability, poor permeability, and poor absorption. Therefore, it is essential and there is a huge market demand for a product with better permeability of active ingredients and more significant efficacy. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the present invention provides a composition with anti-wrinkle and firming effects and its application.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a composition having anti-wrinkle and firming effects, the composition comprising sterols, niacinamide and arginine / lysine peptides;

[0009] The sterols include PEG-10 wild soybean sterol.

[0010] The PEG-10 soybean sterol in the composition of this invention is a nonionic surfactant with a unique amphiphilic structure. It not only improves the stability of the formulation but also promotes the penetration of active ingredients. This ingredient not only has excellent skin conditioning effects, but its unique amphiphilic structure can achieve the best penetration effect of water-soluble nicotinamide and small molecule peptides. Because intercellular lipids and cell membranes are oil-soluble components, while soybean sterol is a sterol substance, its basic structure includes a tetracyclic sterol core and some side chains. These structures have strong lipophilicity and can penetrate better than simple fully water-soluble amino acid sequences.

[0011] Niacinamide is a water-soluble vitamin that can participate in cellular energy metabolism, regulate intracellular redox states, thereby reducing the production of free radicals, stimulate collagen synthesis in skin fibroblasts, inhibit the activity of MMPs, and reduce elastin degradation, thus maintaining skin elasticity and achieving anti-wrinkle and firming effects.

[0012] The arginine / lysine peptide is mainly composed of arginine and lysine, two basic amino acids. It can target and block voltage-dependent sodium ion channels, interacting closely with the target site to effectively block neuromuscular electrical conduction, achieving immediate relaxation of facial skin and restoring skin elasticity. The arginine / lysine peptide works by regulating the interaction between neuromuscular systems, specifically targeting and blocking the NAV1.4 channel in muscles. This blocks neuromuscular electrical conduction, relaxes muscles, and instantly reduces wrinkles, resulting in firmer skin. A relatively high concentration is required for its effectiveness in the human body.

[0013] This invention creatively combines nicotinamide, arginine / lysine peptides, and sterols into a novel composition. The three components exhibit excellent anti-wrinkle and firming effects at low concentrations and have potential synergistic effects.

[0014] Preferably, the composition having anti-wrinkle and firming effects comprises, by weight, 0.01-10 parts of sterols, 0.1-25 parts of nicotinamide, and 0.01-15 parts of arginine / lysine peptides.

[0015] In the anti-wrinkle and firming composition of the present invention, the amount of sterols added can be 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, or 9 parts, etc.

[0016] The amount of nicotinamide added can be 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, or 24 parts, etc.

[0017] The amount of arginine / lysine polypeptide added can be 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, or 14 parts, etc.

[0018] Preferably, the composition having anti-wrinkle and firming effects comprises, by weight, 0.5-5 parts of sterols, 1-20 parts of nicotinamide, and 0.1-10 parts of arginine / lysine peptides.

[0019] Preferably, the sterols also include cholesterol, brassosterols, and phytosterol oleate.

[0020] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0021] In a second aspect, the present invention provides the use of the composition with anti-wrinkle and firming effects according to the first aspect in the preparation of cosmetics.

[0022] Preferably, the cosmetic includes any one of serum, face mask, cream, lotion, or skin care water.

[0023] Preferably, the composition with anti-wrinkle and firming effects has a mass percentage of 1-15% in the cosmetic, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc.

[0024] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0025] Thirdly, the present invention provides an anti-wrinkle and firming serum, wherein the components of the anti-wrinkle and firming serum include the composition with anti-wrinkle and firming effects described in the first aspect, a moisturizer, a thickener, an antioxidant, a skin emollient, and water.

[0026] Preferably, the components of the anti-wrinkle and firming serum, by weight, include 1-10 parts of the anti-wrinkle and firming composition described in the first aspect, 10-25 parts of a moisturizer, 0.1-5 parts of a thickener, 1-5 parts of an antioxidant, 1-10 parts of a skin moisturizer, and 45-90 parts of water.

[0027] The amount of the anti-wrinkle and firming composition added to the essence of the present invention can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.

[0028] The amount of the humectant added can be 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, or 24 parts, etc.

[0029] The amount of the thickener added can be 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc.;

[0030] The amount of antioxidant added can be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc.;

[0031] The amount of the emollient added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, or 9 parts, etc.

[0032] The amount of water added can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, or 85 parts, etc.

[0033] Preferably, the humectant comprises any one or a combination of at least two of glycerin, butylene glycol, dipropylene glycol, or glyceryl polyether-26.

[0034] Preferably, the thickener comprises any one or a combination of at least two of xanthan gum, hydroxyethyl cellulose, Sphingomonas fermentation product extract, carbomer, or acrylate / C10-30 alkanol acrylate crosspolymer.

[0035] Preferably, the antioxidant includes any one or a combination of two of p-hydroxyacetophenone, tocopherol, or tocopheryl acetate.

[0036] Preferably, the emollient comprises any one or a combination of at least two of the following: caprylic / capric triglyceride, shea butter, coconut oil-caprylate / capric acid ester, polydimethylsiloxane, isononyl isononanoate, or hydrogenated polyisobutylene.

[0037] Preferably, the emollient is a combination of caprylic / capric triglyceride, coconut oil alcohol-caprylic / capric ester, isononyl isononanoate and hydrogenated polyisobutylene.

[0038] The emollient of this invention, through the synergistic effect of caprylic / capric triglyceride, coconut oil alcohol-caprylic / capric ester, isononyl isononanoate and hydrogenated polyisobutylene, can enhance the skin barrier function, improve the skin's resistance to the external environment, reduce the damage of external stimuli to the skin, and thus improve the skin's anti-wrinkle and firming effect.

[0039] Preferably, the mass ratio of the caprylic / capric triglyceride, coconut oil alcohol-caprylic / capric ester, isononyl isononyl isononyl isononanoate and hydrogenated polyisobutylene is (0.5-2):(1-4):(2-4):(1-3).

[0040] "0.5-2" can be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.

[0041] "1-4" can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, or 3.8, etc.

[0042] "2-4" can be 2.1, 2.3, 2.5, 2.8, 3, 3.2, 3.5, 3.7, or 3.9, etc.;

[0043] “1-3” can be 1.1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7 or 2.9, etc.

[0044] Preferably, the serum with anti-wrinkle and firming effects also includes skin conditioning agents.

[0045] Preferably, the essence with anti-wrinkle and firming effects further includes 0.1-5 parts of skin conditioning agent by weight, such as 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.

[0046] Preferably, the skin conditioning composition comprises any one or a combination of at least two of dipotassium glycyrrhizate, allantoin, hyaluronic acid, or sodium hyaluronate.

[0047] Preferably, the skin conditioning agent is a combination of dipotassium glycyrrhizate, allantoin, and hyaluronic acid.

[0048] In this invention, the hyaluronic acid provides deep hydration and increases collagen synthesis, allantoin repairs the epidermal barrier and accelerates cell renewal, and dipotassium glycyrrhizate reduces the activity of collagen-degrading enzymes, thereby inhibiting oxidative damage and scavenging free radicals. Together, these three components construct a multi-layered protective network from the epidermis to the dermis, which can better promote the anti-wrinkle and firming effects of the serum.

[0049] Preferably, the mass ratio of dipotassium glycyrrhizate, allantoin, and hyaluronic acid is (0.1-2):(0.1-1.5):(1-3).

[0050] "0.1-2" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.

[0051] "0.1-1.5" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, or 1.4, etc.

[0052] “1-3” can be 1.1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7 or 2.9, etc.

[0053] Preferably, the serum with anti-wrinkle and firming effects further includes any one or a combination of at least two of the following: preservatives, pH adjusters, or fragrances.

[0054] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0055] Fourthly, the present invention provides a method for preparing an anti-wrinkle and firming serum according to the third aspect, the method comprising:

[0056] (1) Mix the humectant, thickener, emollient and water to obtain a mixture;

[0057] (2) The mixture obtained in step (1), the composition with anti-wrinkle and firming effects and the antioxidant are mixed and homogenized to obtain the essence with anti-wrinkle and firming effects.

[0058] Preferably, the mixing temperature in step (1) is 60-70℃ (e.g., 61℃, 63℃, 65℃, 67℃ or 69℃, etc.), and the mixing time is 5-10 min (e.g., 6 min, 7 min, 8 min or 9 min, etc.).

[0059] Preferably, the mixing in step (1) further includes the addition of a skin conditioning agent.

[0060] Preferably, the mixing and homogenization temperature in step (2) is 35-45℃ (e.g., 36℃, 38℃, 40℃, 42℃ or 44℃, etc.), and the mixing time is 20-40min (e.g., 22min, 25min, 28min, 30min, 32min, 35min or 38min, etc.).

[0061] Preferably, step (2) of mixing and homogenizing further includes adding any one or a combination of at least two of the following: preservatives, pH adjusters, or fragrances.

[0062] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0063] Compared with the prior art, the present invention has the following beneficial effects:

[0064] (1) This invention creatively combines nicotinamide, arginine / lysine peptides and sterols into a novel composition. The three components exhibit excellent anti-wrinkle and firming effects at low concentrations and have potential synergistic effects. In particular, PEG-10 soybean sterol can promote the synergistic anti-wrinkle and firming effects of nicotinamide and arginine / lysine peptides. The three components enhance skin elasticity and firmness by promoting the expression of type I collagen genes, enhancing antioxidant capacity and promoting the inhibition rate of elastase.

[0065] (2) In this invention, the hyaluronic acid can provide deep moisturization and increase collagen synthesis, allantoin repairs the epidermal barrier and accelerates cell renewal, and reduces the activity of collagen degradation enzymes. Dipotassium glycyrrhizate can inhibit oxidative damage and remove free radicals. The three together construct a multi-layered protective network from the epidermis to the dermis, which can better promote the anti-wrinkle and firming effect of the essence. Detailed Implementation

[0066] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0067] The sources of some raw materials in the embodiments or comparative examples of this invention are shown in Table 1 below.

[0068] Table 1

[0069]

[0070] All other raw materials can be used as long as they are purchased from authorized distributors.

[0071] Example 1

[0072] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of nicotinamide and 5 parts of arginine / lysine peptides;

[0073] The sterols are a combination of PEG-10 soybean sterol, cholesterol, brassosterols and phytosterol oleate in a mass ratio of 1:0.8:0.5:0.3.

[0074] Example 2

[0075] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 1 part of sterols, 16 parts of nicotinamide and 1 part of arginine / lysine peptides;

[0076] The sterols are a combination of PEG-10 soybean sterol, cholesterol, brassosterols and phytosterol oleate in a mass ratio of 1:0.5:0.3:0.5.

[0077] Example 3

[0078] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 5 parts of sterols, 5 parts of nicotinamide and 8 parts of arginine / lysine peptides;

[0079] The sterols are a combination of PEG-10 soybean sterol, cholesterol, brassosterols and phytosterol oleate in a mass ratio of 1:0.3:0.8:0.2.

[0080] Example 4

[0081] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of nicotinamide and 5 parts of arginine / lysine peptides;

[0082] The sterol is PEG-10 wild soybean sterol.

[0083] Example 5

[0084] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of nicotinamide and 5 parts of arginine / lysine peptides;

[0085] The sterols are a combination of PEG-10 soybean sterol, cholesterol, and brassosterol in a mass ratio of 1:0.8:0.5.

[0086] Example 6

[0087] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of nicotinamide and 5 parts of arginine / lysine peptides;

[0088] The sterols are a combination of PEG-10 soybean sterol, cholesterol, and phytosterol oleate in a mass ratio of 1:0.8:0.3.

[0089] Example 7

[0090] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of nicotinamide and 5 parts of arginine / lysine peptides;

[0091] The sterols are a combination of PEG-10 soybean sterol, rapeseed sterols and phytosterol oleate in a mass ratio of 1:0.5:0.3.

[0092] Comparative Example 1

[0093] This comparative example provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises 10 parts by weight of nicotinamide and 5 parts by weight of arginine / lysine peptide.

[0094] Comparative Example 2

[0095] This comparative example provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises 3 parts by weight of sterols and 5 parts by weight of arginine / lysine peptides.

[0096] The sterols are a combination of PEG-10 soybean sterol, cholesterol, brassosterols and phytosterol oleate in a mass ratio of 1:0.8:0.5:0.3.

[0097] Comparative Example 3

[0098] This comparative example provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises 3 parts by weight of sterols and 10 parts by weight of nicotinamide.

[0099] The sterols are a combination of PEG-10 soybean sterol, cholesterol, brassosterols and phytosterol oleate in a mass ratio of 1:0.8:0.5:0.3.

[0100] Application Examples 1-3

[0101] This application example provides three serums with anti-wrinkle and firming effects. The components of these serums are shown in Table 2 below, and the amounts of each component in the table are parts per serving.

[0102] Table 2

[0103]

[0104] The preparation method of the serum with anti-wrinkle and firming effects is as follows:

[0105] (1) Mix the moisturizer, thickener, emollient, skin conditioner and deionized water at 65°C for 8 min to obtain a mixture;

[0106] (2) The mixture obtained in step (1), the composition with anti-wrinkle and firming effects and the antioxidant are mixed and homogenized at 40°C for 30 min to obtain the essence with anti-wrinkle and firming effects.

[0107] Application Example 4-7

[0108] This application example provides four serums with anti-wrinkle and firming effects. The only difference between this example and application example 1 is that the anti-wrinkle and firming composition provided in example 1 is replaced in equal amounts with the anti-wrinkle and firming compositions provided in examples 4-7. The remaining components and amounts are the same as in application example 1, and the preparation method is the same as in application example 1.

[0109] Application Example 8

[0110] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the emollient does not include caprylic / capric triglyceride. The reduced amount is allocated to coconut oil alcohol-caprylic / capric triglyceride, isononyl isononanoate, and hydrogenated polyisobutylene in proportion to the amount of the other components. The remaining components and their amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0111] Application Example 9

[0112] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the emollient does not include coconut oil alcohol-caprylate / capric acid ester, which is reduced and allocated in proportion to caprylic / capric acid triglyceride, isononyl isononanoate and hydrogenated polyisobutylene. The remaining components and their amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0113] Application Example 10

[0114] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the emollient does not include isononyl isononanoate. The reduced amount is allocated in proportion to caprylic / capric triglyceride, coconut oil alcohol-caprylate / capric acid ester and hydrogenated polyisobutylene. The remaining components and their amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0115] Application Example 11

[0116] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the emollient does not include hydrogenated polyisobutylene. The reduced amount is allocated in parts to caprylic / capric triglyceride, coconut oil alcohol-caprylate / capric acid ester and isononyl isononanoate. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0117] Application Example 12

[0118] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the serum does not contain skin conditioning agents. The reduction is made up by deionized water. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0119] Application Example 13

[0120] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the skin conditioning agent does not include dipotassium glycyrrhizate. The reduced amount is allocated to allantoin and hyaluronic acid in proportion to the amount of glycyrrhizate. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0121] Application Example 14

[0122] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the skin conditioning agent does not contain allantoin. The reduced amount is allocated to dipotassium glycyrrhizate and hyaluronic acid in proportion to the amount of allantoin. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0123] Application Example 15

[0124] This application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the skin conditioning agent does not contain hyaluronic acid. The reduced amount is allocated to dipotassium glycyrrhizate and allantoin in proportion to the amount of hyaluronic acid. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0125] Comparative Application Examples 1-3

[0126] This comparative application example provides three serums with anti-wrinkle and firming effects. The only difference between these and Application Example 1 is that the anti-wrinkle and firming composition provided in Example 1 is replaced in equal amounts with the anti-wrinkle and firming compositions provided in Comparative Examples 1-3. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0127] Comparative Application Example 4

[0128] This comparative application example provides an anti-wrinkle and firming serum, which differs from Application Example 1 only in that the serum does not include an anti-wrinkle and firming composition. The reduction is made up by deionized water. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0129] Test Example 1: Antioxidant Efficacy Study – DPPH Free Radical Scavenging Experiment

[0130] Test samples: Samples provided in Application Examples 1-15 and Comparative Application Examples 1-4;

[0131] Test method:

[0132] Accurately weigh 47.7 mg of DPPH reagent and dilute to a 100 ml amber volumetric flask. Take 5 ml of this flask and dilute to a 50 ml amber volumetric flask with anhydrous ethanol to obtain the DPPH test solution (47.7 mg / L). Transfer 2 mL of the DPPH test solution to each of the following samples: 0.1 mL of the samples provided in Application Examples 1-15 and Comparative Application Examples 1-4, as shown in Table 3. Add an appropriate amount of anhydrous ethanol to ensure equal total mass. After thorough mixing, allow to stand in the dark for 30 min. Using anhydrous ethanol as a blank control, measure the absorbance at 517 nm. Vitamin C is used as a control. Calculate the DPPH scavenging rate of each extract using the following formula:

[0133] DPPH clearance rate (%) = [1 - (As - Ar) / A0] × 100%

[0134] Where: As—absorbance of the reaction system of extract and DPPH; Ar—absorbance of extract-anhydrous ethanol; A0—absorbance of blank control anhydrous ethanol.

[0135] Table 3

[0136]

[0137] The DPPH free radical scavenging rate reflects an antioxidant capacity to some extent; a higher scavenging rate indicates better antioxidant activity and thus a better anti-aging effect. Therefore, the anti-aging effect of a serum can be judged by studying its ability to scavenge DPPH free radicals. Vitamin C is a recognized high-efficiency antioxidant in the cosmetics industry. This experiment used Vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 4.

[0138] Table 4

[0139]

[0140] As shown in Table 4, compared with Comparative Application Example 4, the samples in Application Examples 1-3 all showed better removal effects on DPPH, with removal rates all above 60%.

[0141] As can be seen from Application Examples 4-7, the anti-wrinkle and firming composition of the present invention can better improve the antioxidant effect of the product by adding cholesterol, brassicosterols and phytosterol oleate, and the three have a synergistic effect.

[0142] The reduced DPPH removal effect in Application Examples 12-15 indicates that adding skin conditioning agents to the serum can improve the product's antioxidant effect, and the synergistic effect of dipotassium glycyrrhizate, allantoin, and hyaluronic acid can further enhance the product's antioxidant effect.

[0143] The decrease in DPPH removal effect observed in Comparative Application Examples 1-3 indicates that the sterols, nicotinamide, and arginine / lysine peptides in the anti-wrinkle and firming composition of the present invention mutually promote each other in terms of antioxidant effects, resulting in a synergistic effect.

[0144] Test Example 2: Anti-wrinkle and skin-firming test

[0145] The skin-firming effect of the serums prepared in corresponding use cases 1-15 and comparative application examples 1-4 was evaluated:

[0146] 133 participants aged 20-45 were randomly selected and divided into 19 groups of 7. Each group applied the serum prepared in Examples 1-15 and the control examples 1-4 to the left and right sides of their faces respectively (once in the morning and once in the evening) for 28 days. Participants were prohibited from using any other skin-maintaining products or cosmetics that might affect the results during the trial period, and their original dietary habits remained unchanged.

[0147] Skin condition was tested on day 0 and day 28 using a Cutometer MPA580 skin elastometer. The test site was the cheekbone area. Measurements were taken three times on the same area, and the average value was recorded. The test parameter was R² (the ratio of skin rebound Ua without negative pressure to the maximum stretch Uf under negative pressure). The closer R² is to 1, the better the skin elasticity. The average change in R² = R² value on day 28 - R² value on day 0, and the average rate of change in R² = average change in R² / R² value on day 0. The specific skin elasticity test results are shown in Table 5.

[0148] Table 5

[0149]

[0150] As shown in Table 5, compared with Comparative Application Example 4, the serums involved in Examples 1-3 of the present invention have better skin-tightening effects, indicating that the composition with anti-wrinkle and firming effects provided by the present invention can significantly improve the skin-tightening effect of the product. Furthermore, as can be seen from Comparative Application Examples 1-3, the three components of sterols, niacinamide, and arginine / lysine peptides promote each other in this effect and have an unexpected synergistic effect.

[0151] Test Example 3: Anti-wrinkle and firming test

[0152] The anti-wrinkle and firming effects of samples prepared according to use cases 1-15 and comparative application examples 1-5 were evaluated:

[0153] The specific testing method is as follows:

[0154] (1) Upregulation rate of type I collagen (COL1AI) and elastin (ELN) expression in human dermal fibroblasts:

[0155] Test Principle: Fibroblasts synthesize and secrete collagen and elastin, forming collagen fibers, reticular fibers, and elastic fibers. They also synthesize and secrete matrix components such as glycosaminoglycans and glycoproteins. Type I and II collagen are among the main components of the dermal extracellular matrix. In infant and adolescent skin, type I collagen accounts for approximately 70% of the content; the appearance of skin wrinkles is closely related to the normal synthesis and expression of collagen. Elastin, present in the extracellular matrix, plays a crucial role in maintaining skin elasticity and is a major cause of aging phenomena such as skin laxity and sagging. Ultraviolet radiation leads to rough, thickened, and inelastic skin, resulting in wrinkles. Skin fragility is attributed to changes in hemidesmosomes and the downregulation of various collagen proteins (such as Collagen I) expression at the dermal-epidermal junction. This experiment utilizes human dermal fibroblasts. After applying the sample to the cells, the relative expression levels of the COL1AI and ELN genes are detected to evaluate whether the sample has anti-wrinkle and firming effects.

[0156] Experimental steps:

[0157] (1) Seed cells in 6-well plates (3x10⁻¹²) 5 (each well) was incubated at 37°C with 5% CO2 for 24 hours.

[0158] (2) After incubation, remove the culture medium, wash with D-Hanks 1-2 times, and then subject the sample group and the model control group to UVA irradiation (9J / cm²).

[0159] (3) After the modeling was completed, the normal control group and the model control group were replaced with fresh culture medium. Fresh culture medium containing the sample was added to the sample wells and incubated at 37°C and 5% CO2 for 24 h.

[0160] (4) Total RNA was extracted from each experimental group, cDNA was synthesized, and the gene expression of β-actin and the target gene was detected by q-PCR.

[0161] (5) Using β-actin as an internal reference for gene expression, calculate the relative RNA expression level of the target gene.

[0162] relative RNA expression level = 2 ΔΔC(t) ;

[0163] Where, ΔΔC(t) = ΔC(t) 模型对照组 -ΔC(t) 样品组 ;

[0164] ΔC(t) = C(t) 目的基因 -C(t) β-actin ;

[0165] Upward adjustment rate (%) = x100%

[0166] In the formula, P represents the relative expression levels of the COL1AI and ELN genes.

[0167] Statistical results are expressed as mean ± SD. The independent samples t-test was used for statistical analysis between the two groups. The statistical results were defined with α = 0.05 as the test cutoff, and p < 0.05 (compared to the model control group) was considered statistically significant. Compared with the model control group, the relative expression levels of COL1AI and ELN genes in the sample group were significantly upregulated, indicating that the sample has anti-wrinkle and firming effects.

[0168] (2) Determination of the anti-wrinkle and firming effects of elastase activity inhibition

[0169] Test Principle: Elastic fibers, composed of elastin and microfibrils, are distributed in the dermis and subcutaneous tissue, giving the skin its elasticity. Environmental factors such as ultraviolet radiation, stress, and pollution can promote the production of elastase in the body. Elastase, a member of the chymotrypsin family, degrades elastin, causing the loss of connective tissue in the epidermis, thus leading to skin aging, wrinkles, and photoaging.

[0170] Through experimental design, the results of elastase inhibition rate tests were compared between the sample group and the blank control group. If the inhibition rate of the sample group was better than that of the blank control group and the statistical difference P value was <0.05, the sample group could be considered to have certain anti-wrinkle and firming effects.

[0171] Test method:

[0172] (1) Weighing the sample;

[0173] (2) Pre-treat the sample group to prepare a mixed solution;

[0174] (3) Incubate at 37°C for a period of time; then add a certain amount of elastase and continue incubation;

[0175] (4) Test the elastase inhibition rate (OD value) on the machine;

[0176] (5) Record the data and calculate the inhibition rate and statistical difference P-value;

[0177] The specific test results are shown in Table 6 below:

[0178] Table 6

[0179]

[0180] As shown in Table 6, compared with Comparative Application Example 4, the serums of Application Examples 1-3 of the present invention showed significant improvements in the upregulation rate of type I collagen (COL1AI) expression (over 80%), the upregulation rate of elastin (ELN gene) expression (over 58%), and the inhibition rate of elastase (over 28%). This indicates that the composition with anti-wrinkle and firming effects provided by the present invention has significant anti-aging effects.

[0181] As can be seen from the comparison of application examples 4-7, the anti-wrinkle and firming composition of the present invention can better enhance the anti-aging effect of the product by adding cholesterol, brassicasterols and phytosterol oleate, and the three have a synergistic effect.

[0182] As can be seen from the comparison of application examples 8-11, the choice of the emollient also affects the expression of type I collagen (COL1AI), the expression of elastin (ELN gene), and the inhibitory effect of elastase.

[0183] As can be seen from Application Examples 1 and 12-15, adding a skin conditioning agent to the serum of the present invention can enhance the anti-aging effect of the product, and the formulation of the skin conditioning agent also affects the anti-aging effect of the serum.

[0184] As can be seen from the comparison between Application Example 1 and Comparative Application Examples 1-3, the sterols, nicotinamide and arginine / lysine peptides have an unexpected synergistic effect in anti-aging efficacy.

[0185] Test Example 4: Safety Evaluation Experiment

[0186] The specific method was as follows: Forty women aged 18-35 years with no history of allergies were selected. Each subject used samples prepared according to Examples 1-15. After cleaning the subject's back, the patch applicator containing the sample was applied to the selected location on the back using non-irritating adhesive tape. After application, the patch was gently pressed with the fingers to ensure even application to the skin, and left for 48 hours. During these 48 hours, the subject kept the patch area dry and avoided strenuous exercise, scratching, and prolonged sun exposure. After 48 hours, the applicator was removed and marked. After 30 minutes, once the pressure marks disappeared, the subjects were assessed under sufficient light.

[0187] The grading criteria for adverse skin reactions are shown in Table 7:

[0188] Table 7

[0189]

[0190] The results are shown in Table 8:

[0191] Table 8

[0192]

[0193] As shown in Table 8, no adverse skin reactions were observed in the volunteers after the spot tester was removed. This indicates that the raw materials used in the essence of this invention are safe and non-irritating, have no adverse reactions on the human body, and are highly safe.

[0194] The applicant declares that the present invention is illustrated by the above embodiments to demonstrate a composition with anti-wrinkle and firming effects and its application, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0195] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0196] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A composition having anti-wrinkle and firming effects, characterized in that, By weight, the composition having anti-wrinkle and firming effects comprises 1-5 parts of sterols, 5-16 parts of nicotinamide, and 1-8 parts of arginine / lysine peptides; The sterols are PEG-10 soybean sterol, cholesterol, brassosterols and phytosterol oleate.

2. The use of the composition with anti-wrinkle and firming effects according to claim 1 in the preparation of cosmetics.

3. The application according to claim 2, characterized in that, The cosmetics include any one of serums, face masks, creams, lotions, or skin care waters; The composition having anti-wrinkle and firming effects has a mass percentage of 1-15% in cosmetics.

4. A serum with anti-wrinkle and firming effects, characterized in that, The components of the serum with anti-wrinkle and firming effects include the composition with anti-wrinkle and firming effects as described in claim 1, a moisturizer, a thickener, an antioxidant, a skin emollient, and water.

5. The serum with anti-wrinkle and firming effects according to claim 4, characterized in that, The components of the serum with anti-wrinkle and firming effects, by weight, include 1-10 parts of the composition with anti-wrinkle and firming effects as described in claim 1, 10-25 parts of moisturizer, 0.1-5 parts of thickener, 1-5 parts of antioxidant, 1-10 parts of emollient, and 45-90 parts of water.

6. The serum with anti-wrinkle and firming effects according to claim 5, characterized in that, The moisturizer includes any one or a combination of at least two of glycerin, butylene glycol, dipropylene glycol, or glyceryl polyether-26; The thickener includes any one or a combination of at least two of xanthan gum, hydroxyethyl cellulose, Sphingomonas fermentation product extract, carbomer, or acrylate / C10-30 alkanol acrylate crosspolymers; The antioxidants include any one or a combination of two of p-hydroxyacetophenone, tocopherol, or tocopheryl acetate. The emollient is a combination of caprylic / capric triglyceride, coconut oil alcohol-caprylic / capric ester, isononyl isononanoate and hydrogenated polyisobutylene.

7. The serum with anti-wrinkle and firming effects according to claim 6, characterized in that, The serum with anti-wrinkle and firming effects also includes 0.1-5 parts of skin conditioning agent by weight; The skin conditioning agent is a combination of dipotassium glycyrrhizate, allantoin, and hyaluronic acid. The serum with anti-wrinkle and firming effects also includes any one or a combination of at least two of the following: preservatives, pH adjusters, or fragrances.

8. A method for preparing an anti-wrinkle and firming serum according to claim 7, characterized in that, The preparation method includes: (1) Mix the moisturizer, thickener, emollient, skin conditioner and water to obtain a mixture; the mixing temperature in step (1) is 60-70℃ and the mixing time is 5-10 min; (2) The mixture obtained in step (1), the composition with anti-wrinkle and firming effects and the antioxidant are mixed and homogenized to obtain the essence with anti-wrinkle and firming effects; The mixing and homogenization temperature in step (2) is 35-45℃, and the mixing and homogenization time is 20-40 min; Step (2) mixing and homogenizing also includes adding any one or a combination of at least two of the following: preservatives, pH adjusters, or fragrances.

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